3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G77680 |
| fragaria_vesca | FvH4_2g26510 |
| malus_domestica | MD08G1168200.v1.1 MD15G1353600.v1.1 |
| prunus_persica | Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 |
| pyrus_communis | pycom08g14370 pycom15g31690 pycom15g31710 |
| rosa_chinensis | RchiOBHm_Chr6g0295321 |
| rosa_laevigata | RLG00000011813 |
| rosa_multiflora | Rmu_sc0012530.1_g000002 Rmu_sc0016917.1_g000001 |
| rosa_roxburghii | Rroxscaffold_171G00441480 Rroxscaffold_7G00172580 |
| rosa_rugosa | Rorug06G0250000 |
| rosa_samantha | Rh6AG363200 Rh6BG371300 Rh6CG377800 Rh6DG363900 |
| rosa_wichuraiana | Rw6G031690 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 575 |
| Acc16I | TGCGCA | 1 cut(s) | 1460 |
| Acc36I | ACCTGC | 1 cut(s) | 575 |
| Acc65I | GGTACC | 1 cut(s) | 126 |
| AccB1I | GGYRCC | 4 cut(s) | 126, 691, 1440, 2345 |
| AccI | GTMKAC | 1 cut(s) | 1999 |
| AciI | CCGC | 5 cut(s) | 919, 1051, 1391, 1435, 2319 |
| AclWI | GGATC | 2 cut(s) | 729, 2384 |
| AcoI | YGGCCR | 3 cut(s) | 577, 1048, 1168 |
| AcsI | RAATTY | 6 cut(s) | 625, 810, 1218, 1348, 1472, 2209 |
| AcuI | CTGAAG | 4 cut(s) | 123, 1247, 1416, 2066 |
| AdeI | CACNNNGTG | 1 cut(s) | 2417 |
| AfaI | GTAC | 6 cut(s) | 57, 128, 544, 594, 1703, 2495 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 34, 1024, 2342 |
| AflIII | ACRYGT | 2 cut(s) | 2002, 2031 |
| AjiI | CACGTC | 1 cut(s) | 278 |
| AjnI | CCWGG | 3 cut(s) | 63, 973, 2248 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 797, 829, 1106, 1138 |
| AleI | CACNNNNGTG | 1 cut(s) | 2201 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1677, 1709 |
| AluBI | AGCT | 9 cut(s) | 557, 884, 1267, 1412, 1564, 1990, 2026, 2125, 2309 |
| AluI | AGCT | 9 cut(s) | 557, 884, 1267, 1412, 1564, 1990, 2026, 2125, 2309 |
| Alw21I | GWGCWC | 2 cut(s) | 948, 1566 |
| Alw26I | GTCTC | 4 cut(s) | 276, 276, 979, 1798 |
| AlwI | GGATC | 2 cut(s) | 729, 2384 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2435 |
| AoxI | GGCC | 5 cut(s) | 577, 1048, 1168, 2037, 2093 |
| ApaI | GGGCCC | 1 cut(s) | 2041 |
| ApeKI | GCWGC | 7 cut(s) | 512, 1644, 1763, 1766, 1916, 1919, 1967 |
| ApoI | RAATTY | 6 cut(s) | 625, 810, 1218, 1348, 1472, 2209 |
| AseI | ATTAAT | 1 cut(s) | 633 |
| Asp718I | GGTACC | 1 cut(s) | 126 |
| AspLEI | GCGC | 2 cut(s) | 1461, 2266 |
| AspS9I | GGNCC | 6 cut(s) | 433, 2037, 2038, 2093, 2353, 2468 |
| AsuC2I | CCSGG | 1 cut(s) | 581 |
| AsuHPI | GGTGA | 4 cut(s) | 79, 179, 494, 599 |
| AsuII | TTCGAA | 1 cut(s) | 727 |
| AvaII | GGWCC | 3 cut(s) | 433, 2353, 2468 |
| AxyI | CCTNAGG | 2 cut(s) | 47, 696 |
| BaeGI | GKGCMC | 1 cut(s) | 2041 |
| BalI | TGGCCA | 1 cut(s) | 1170 |
| BanI | GGYRCC | 4 cut(s) | 126, 691, 1440, 2345 |
| BanII | GRGCYC | 5 cut(s) | 40, 1261, 1277, 1566, 2041 |
| BbsI | GAAGAC | 2 cut(s) | 662, 1512 |
| Bbv12I | GWGCWC | 2 cut(s) | 948, 1566 |
| BbvI | GCAGC | 7 cut(s) | 499, 1656, 1750, 1778, 1903, 1931, 1954 |
| BccI | CCATC | 4 cut(s) | 755, 1035, 1247, 2459 |
| BceAI | ACGGC | 4 cut(s) | 150, 1165, 1754, 2333 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 98, 132, 717, 751 |
| BciT130I | CCWGG | 3 cut(s) | 65, 975, 2250 |
| BciVI | GTATCC | 3 cut(s) | 836, 1504, 1743 |
| BclI | TGATCA | 1 cut(s) | 213 |
| BcnI | CCSGG | 1 cut(s) | 581 |
| BcoDI | GTCTC | 4 cut(s) | 276, 276, 979, 1798 |
| BfaI | CTAG | 6 cut(s) | 378, 891, 1407, 1995, 2097, 2315 |
| BfmI | CTRYAG | 3 cut(s) | 1764, 1917, 2436 |
| BfoI | RGCGCY | 1 cut(s) | 2267 |
| BfuAI | ACCTGC | 1 cut(s) | 575 |
| BfuI | GTATCC | 3 cut(s) | 836, 1504, 1743 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1775 |
| BglII | AGATCT | 2 cut(s) | 1196, 1207 |
| BisI | GCNGC | 9 cut(s) | 513, 1051, 1392, 1645, 1764, 1767, 1917, 1920, 1968 |
| BlsI | GCNGC | 9 cut(s) | 514, 1052, 1393, 1646, 1765, 1768, 1918, 1921, 1969 |
| BmcAI | AGTACT | 2 cut(s) | 57, 544 |
| Bme1390I | CCNGG | 4 cut(s) | 65, 581, 975, 2250 |
| Bme18I | GGWCC | 3 cut(s) | 433, 2353, 2468 |
| BmgBI | CACGTC | 1 cut(s) | 278 |
| BmgT120I | GGNCC | 6 cut(s) | 433, 2037, 2038, 2093, 2353, 2468 |
| BmiI | GGNNCC | 7 cut(s) | 128, 693, 1442, 2039, 2094, 2347, 2470 |
| BmrFI | CCNGG | 4 cut(s) | 65, 581, 975, 2250 |
| BmsI | GCATC | 5 cut(s) | 769, 1564, 1759, 2002, 2176 |
| BpiI | GAAGAC | 2 cut(s) | 662, 1512 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 22, 54 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1095 |
| Bpu14I | TTCGAA | 1 cut(s) | 727 |
| BpuEI | CTTGAG | 3 cut(s) | 665, 1289, 1550 |
| BpuMI | CCSGG | 1 cut(s) | 581 |
| Bsa29I | ATCGAT | 1 cut(s) | 108 |
| BsaI | GGTCTC | 1 cut(s) | 276 |
| BsaJI | CCNNGG | 6 cut(s) | 132, 973, 974, 2280, 2349, 2356 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1234, 1264, 2230, 2260 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 34, 1024, 2342 |
| Bse1I | ACTGG | 4 cut(s) | 247, 1861, 2329, 2393 |
| Bse21I | CCTNAGG | 2 cut(s) | 47, 696 |
| Bse3DI | GCAATG | 3 cut(s) | 831, 1541, 1984 |
| BseBI | CCWGG | 3 cut(s) | 65, 975, 2250 |
| BseCI | ATCGAT | 1 cut(s) | 108 |
| BseDI | CCNNGG | 6 cut(s) | 132, 973, 974, 2280, 2349, 2356 |
| BseGI | GGATG | 8 cut(s) | 538, 781, 1033, 1576, 1837, 2017, 2192, 2467 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 34, 1024, 2342 |
| BseMI | GCAATG | 3 cut(s) | 831, 1541, 1984 |
| BseNI | ACTGG | 4 cut(s) | 247, 1861, 2329, 2393 |
| BseRI | GAGGAG | 2 cut(s) | 1376, 1796 |
| BseSI | GKGCMC | 1 cut(s) | 2041 |
| BseXI | GCAGC | 7 cut(s) | 499, 1656, 1750, 1778, 1903, 1931, 1954 |
| BseYI | CCCAGC | 1 cut(s) | 119 |
| BshFI | GGCC | 5 cut(s) | 579, 1050, 1170, 2039, 2095 |
| BshNI | GGYRCC | 4 cut(s) | 126, 691, 1440, 2345 |
| BshVI | ATCGAT | 1 cut(s) | 108 |
| BsiHKAI | GWGCWC | 2 cut(s) | 948, 1566 |
| BsiSI | CCGG | 1 cut(s) | 580 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 34, 1024, 2342 |
| BsmAI | GTCTC | 4 cut(s) | 276, 276, 979, 1798 |
| BsmI | GAATGC | 2 cut(s) | 390, 574 |
| BsnI | GGCC | 5 cut(s) | 579, 1050, 1170, 2039, 2095 |
| Bso31I | GGTCTC | 1 cut(s) | 276 |
| Bsp119I | TTCGAA | 1 cut(s) | 727 |
| Bsp120I | GGGCCC | 1 cut(s) | 2037 |
| Bsp1286I | GDGCHC | 6 cut(s) | 40, 948, 1261, 1277, 1566, 2041 |
| Bsp1407I | TGTACA | 2 cut(s) | 1701, 2493 |
| Bsp143I | GATC | 9 cut(s) | 109, 213, 721, 769, 893, 1064, 1196, 1207, 2389 |
| BspACI | CCGC | 5 cut(s) | 919, 1051, 1391, 1435, 2319 |
| BspANI | GGCC | 5 cut(s) | 579, 1050, 1170, 2039, 2095 |
| BspDI | ATCGAT | 1 cut(s) | 108 |
| BspHI | TCATGA | 1 cut(s) | 652 |
| BspLI | GGNNCC | 7 cut(s) | 128, 693, 1442, 2039, 2094, 2347, 2470 |
| BspMAI | CTGCAG | 2 cut(s) | 1768, 1921 |
| BspMI | ACCTGC | 1 cut(s) | 575 |
| BspPI | GGATC | 2 cut(s) | 729, 2384 |
| BspQI | GCTCTTC | 2 cut(s) | 1266, 1554 |
| BspT104I | TTCGAA | 1 cut(s) | 727 |
| BspT107I | GGYRCC | 4 cut(s) | 126, 691, 1440, 2345 |
| BspTNI | GGTCTC | 1 cut(s) | 276 |
| BsrDI | GCAATG | 3 cut(s) | 831, 1541, 1984 |
| BsrGI | TGTACA | 2 cut(s) | 1701, 2493 |
| BsrI | ACTGG | 4 cut(s) | 247, 1861, 2329, 2393 |
| BssECI | CCNNGG | 6 cut(s) | 132, 973, 974, 2280, 2349, 2356 |
| BssMI | GATC | 9 cut(s) | 109, 213, 721, 769, 893, 1064, 1196, 1207, 2389 |
| BssT1I | CCWWGG | 2 cut(s) | 2280, 2356 |
| Bst2UI | CCWGG | 3 cut(s) | 65, 975, 2250 |
| Bst4CI | ACNGT | 4 cut(s) | 870, 1057, 1809, 2431 |
| Bst6I | CTCTTC | 3 cut(s) | 461, 1266, 1554 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1543, 1913 |
| BstAUI | TGTACA | 2 cut(s) | 1701, 2493 |
| BstBI | TTCGAA | 1 cut(s) | 727 |
| BstC8I | GCNNGC | 5 cut(s) | 22, 1757, 1761, 1992, 2127 |
| BstDSI | CCRYGG | 2 cut(s) | 132, 2349 |
| BstF5I | GGATG | 8 cut(s) | 538, 781, 1033, 1576, 1837, 2017, 2192, 2467 |
| BstH2I | RGCGCY | 1 cut(s) | 2267 |
| BstHHI | GCGC | 2 cut(s) | 1461, 2266 |
| BstKTI | GATC | 9 cut(s) | 112, 216, 724, 772, 896, 1067, 1199, 1210, 2392 |
| BstMAI | GTCTC | 4 cut(s) | 276, 276, 979, 1798 |
| BstMBI | GATC | 9 cut(s) | 109, 213, 721, 769, 893, 1064, 1196, 1207, 2389 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 554, 943, 1534, 1543, 1756, 1775, 1913 |
| BstNI | CCWGG | 3 cut(s) | 65, 975, 2250 |
| BstNSI | RCATGY | 4 cut(s) | 1094, 1327, 2006, 2035 |
| BstSCI | CCNGG | 4 cut(s) | 63, 579, 973, 2248 |
| BstSFI | CTRYAG | 3 cut(s) | 1764, 1917, 2436 |
| BstSLI | GKGCMC | 1 cut(s) | 2041 |
| BstV1I | GCAGC | 7 cut(s) | 499, 1656, 1750, 1778, 1903, 1931, 1954 |
| BstV2I | GAAGAC | 2 cut(s) | 662, 1512 |
| BstX2I | RGATCY | 3 cut(s) | 721, 1196, 1207 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2363 |
| BstYI | RGATCY | 3 cut(s) | 721, 1196, 1207 |
| Bsu15I | ATCGAT | 1 cut(s) | 108 |
| Bsu36I | CCTNAGG | 2 cut(s) | 47, 696 |
| BsuI | GTATCC | 3 cut(s) | 836, 1504, 1743 |
| BsuRI | GGCC | 5 cut(s) | 579, 1050, 1170, 2039, 2095 |
| BsuTUI | ATCGAT | 1 cut(s) | 108 |
| BtgI | CCRYGG | 2 cut(s) | 132, 2349 |
| BtgZI | GCGATG | 1 cut(s) | 1736 |
| BtrI | CACGTC | 1 cut(s) | 278 |
| BtsCI | GGATG | 8 cut(s) | 538, 781, 1033, 1576, 1837, 2017, 2192, 2467 |
| BtsI | GCAGTG | 1 cut(s) | 1721 |
| BtsIMutI | CAGTG | 4 cut(s) | 1053, 1721, 1854, 2400 |
| BveI | ACCTGC | 1 cut(s) | 575 |
| Cac8I | GCNNGC | 5 cut(s) | 22, 1757, 1761, 1992, 2127 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2435 |
| CciI | TCATGA | 1 cut(s) | 652 |
| CfoI | GCGC | 2 cut(s) | 1461, 2266 |
| Cfr13I | GGNCC | 6 cut(s) | 433, 2037, 2038, 2093, 2353, 2468 |
| ClaI | ATCGAT | 1 cut(s) | 108 |
| CseI | GACGC | 2 cut(s) | 1715, 2235 |
| Csp6I | GTAC | 6 cut(s) | 56, 127, 543, 593, 1702, 2494 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 411, 446 |
| CviQI | GTAC | 6 cut(s) | 56, 127, 543, 593, 1702, 2494 |
| DpnI | GATC | 9 cut(s) | 111, 215, 723, 771, 895, 1066, 1198, 1209, 2391 |
| DpnII | GATC | 9 cut(s) | 109, 213, 721, 769, 893, 1064, 1196, 1207, 2389 |
| DraIII | CACNNNGTG | 1 cut(s) | 2417 |
| EaeI | YGGCCR | 3 cut(s) | 577, 1048, 1168 |
| Eam1104I | CTCTTC | 3 cut(s) | 461, 1266, 1554 |
| EarI | CTCTTC | 3 cut(s) | 461, 1266, 1554 |
| EciI | GGCGGA | 1 cut(s) | 2334 |
| Ecl136II | GAGCTC | 1 cut(s) | 1564 |
| Eco130I | CCWWGG | 2 cut(s) | 2280, 2356 |
| Eco24I | GRGCYC | 5 cut(s) | 40, 1261, 1277, 1566, 2041 |
| Eco31I | GGTCTC | 1 cut(s) | 276 |
| Eco47I | GGWCC | 3 cut(s) | 433, 2353, 2468 |
| Eco53kI | GAGCTC | 1 cut(s) | 1564 |
| Eco57I | CTGAAG | 4 cut(s) | 123, 1247, 1416, 2066 |
| Eco81I | CCTNAGG | 2 cut(s) | 47, 696 |
| EcoICRI | GAGCTC | 1 cut(s) | 1564 |
| EcoO109I | RGGNCCY | 2 cut(s) | 2038, 2093 |
| EcoRII | CCWGG | 3 cut(s) | 63, 973, 2248 |
| EcoT14I | CCWWGG | 2 cut(s) | 2280, 2356 |
| EcoT22I | ATGCAT | 2 cut(s) | 370, 740 |
| EcoT38I | GRGCYC | 5 cut(s) | 40, 1261, 1277, 1566, 2041 |
| ErhI | CCWWGG | 2 cut(s) | 2280, 2356 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1197, 1229 |
| FauNDI | CATATG | 1 cut(s) | 1315 |
| FbaI | TGATCA | 1 cut(s) | 213 |
| FblI | GTMKAC | 1 cut(s) | 1999 |
| Fnu4HI | GCNGC | 9 cut(s) | 513, 1051, 1392, 1645, 1764, 1767, 1917, 1920, 1968 |
| FokI | GGATG | 8 cut(s) | 545, 788, 1040, 1583, 1844, 2024, 2179, 2474 |
| FriOI | GRGCYC | 5 cut(s) | 40, 1261, 1277, 1566, 2041 |
| Fsp4HI | GCNGC | 9 cut(s) | 513, 1051, 1392, 1645, 1764, 1767, 1917, 1920, 1968 |
| FspBI | CTAG | 6 cut(s) | 378, 891, 1407, 1995, 2097, 2315 |
| FspI | TGCGCA | 1 cut(s) | 1460 |
| GlaI | GCGC | 2 cut(s) | 1460, 2265 |
| GluI | GCNGC | 9 cut(s) | 513, 1051, 1392, 1645, 1764, 1767, 1917, 1920, 1968 |
| GsaI | CCCAGC | 1 cut(s) | 123 |
| HaeII | RGCGCY | 1 cut(s) | 2267 |
| HaeIII | GGCC | 5 cut(s) | 579, 1050, 1170, 2039, 2095 |
| HapII | CCGG | 1 cut(s) | 580 |
| HgaI | GACGC | 2 cut(s) | 1715, 2235 |
| HhaI | GCGC | 2 cut(s) | 1461, 2266 |
| Hin6I | GCGC | 2 cut(s) | 1459, 2264 |
| HinP1I | GCGC | 2 cut(s) | 1459, 2264 |
| HindIII | AAGCTT | 1 cut(s) | 2123 |
| HinfI | GANTC | 9 cut(s) | 70, 284, 455, 488, 656, 1343, 1551, 1886, 2100 |
| HpaII | CCGG | 1 cut(s) | 580 |
| HphI | GGTGA | 4 cut(s) | 79, 179, 494, 599 |
| Hpy166II | GTNNAC | 2 cut(s) | 1704, 2000 |
| Hpy8I | GTNNAC | 2 cut(s) | 1704, 2000 |
| Hpy99I | CGWCG | 4 cut(s) | 282, 1519, 1731, 2142 |
| HpyAV | CCTTC | 7 cut(s) | 149, 237, 311, 765, 1228, 1333, 2156 |
| HpyCH4III | ACNGT | 4 cut(s) | 870, 1057, 1809, 2431 |
| HpyCH4IV | ACGT | 3 cut(s) | 277, 1514, 2137 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 554, 943, 1534, 1543, 1756, 1775, 1913 |
| HpySE526I | ACGT | 3 cut(s) | 277, 1514, 2137 |
| HspAI | GCGC | 2 cut(s) | 1459, 2264 |
| KpnI | GGTACC | 1 cut(s) | 130 |
| Ksp22I | TGATCA | 1 cut(s) | 213 |
| Kzo9I | GATC | 9 cut(s) | 109, 213, 721, 769, 893, 1064, 1196, 1207, 2389 |
| LguI | GCTCTTC | 2 cut(s) | 1266, 1554 |
| LmnI | GCTCC | 1 cut(s) | 553 |
| Lsp1109I | GCAGC | 7 cut(s) | 499, 1656, 1750, 1778, 1903, 1931, 1954 |
| LweI | GCATC | 5 cut(s) | 769, 1564, 1759, 2002, 2176 |
| MaeI | CTAG | 6 cut(s) | 378, 891, 1407, 1995, 2097, 2315 |
| MaeII | ACGT | 3 cut(s) | 277, 1514, 2137 |
| MaeIII | GTNAC | 3 cut(s) | 380, 912, 1676 |
| MalI | GATC | 9 cut(s) | 111, 215, 723, 771, 895, 1066, 1198, 1209, 2391 |
| MboI | GATC | 9 cut(s) | 109, 213, 721, 769, 893, 1064, 1196, 1207, 2389 |
| MfeI | CAATTG | 1 cut(s) | 89 |
| MflI | RGATCY | 3 cut(s) | 721, 1196, 1207 |
| MhlI | GDGCHC | 6 cut(s) | 40, 948, 1261, 1277, 1566, 2041 |
| MlsI | TGGCCA | 1 cut(s) | 1170 |
| MluNI | TGGCCA | 1 cut(s) | 1170 |
| MlyI | GAGTC | 3 cut(s) | 278, 497, 1337 |
| Mox20I | TGGCCA | 1 cut(s) | 1170 |
| Mph1103I | ATGCAT | 2 cut(s) | 370, 740 |
| MscI | TGGCCA | 1 cut(s) | 1170 |
| MseI | TTAA | 9 cut(s) | 309, 633, 743, 969, 1119, 1203, 1652, 1839, 2168 |
| MslI | CAYNNNNRTG | 2 cut(s) | 395, 2201 |
| Msp20I | TGGCCA | 1 cut(s) | 1170 |
| MspA1I | CMGCKG | 1 cut(s) | 919 |
| MspI | CCGG | 1 cut(s) | 580 |
| MspR9I | CCNGG | 4 cut(s) | 65, 581, 975, 2250 |
| MunI | CAATTG | 1 cut(s) | 89 |
| Mva1269I | GAATGC | 2 cut(s) | 390, 574 |
| MvaI | CCWGG | 3 cut(s) | 65, 975, 2250 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 554, 943, 1534, 1543, 1756, 1775, 1913 |
| NciI | CCSGG | 1 cut(s) | 581 |
| NdeI | CATATG | 1 cut(s) | 1315 |
| NdeII | GATC | 9 cut(s) | 109, 213, 721, 769, 893, 1064, 1196, 1207, 2389 |
| NlaIV | GGNNCC | 7 cut(s) | 128, 693, 1442, 2039, 2094, 2347, 2470 |
| NmuCI | GTSAC | 3 cut(s) | 380, 912, 1676 |
| NsbI | TGCGCA | 1 cut(s) | 1460 |
| NsiI | ATGCAT | 2 cut(s) | 370, 740 |
| NspI | RCATGY | 4 cut(s) | 1094, 1327, 2006, 2035 |
| NspV | TTCGAA | 1 cut(s) | 727 |
| OliI | CACNNNNGTG | 1 cut(s) | 2201 |
| PagI | TCATGA | 1 cut(s) | 652 |
| PaqCI | CACCTGC | 1 cut(s) | 575 |
| PasI | CCCWGGG | 1 cut(s) | 974 |
| PciI | ACATGT | 2 cut(s) | 2002, 2031 |
| PciSI | GCTCTTC | 2 cut(s) | 1266, 1554 |
| PctI | GAATGC | 2 cut(s) | 390, 574 |
| PfeI | GAWTC | 6 cut(s) | 70, 455, 656, 1551, 1886, 2100 |
| PkrI | GCNGC | 9 cut(s) | 514, 1052, 1393, 1646, 1765, 1768, 1918, 1921, 1969 |
| PleI | GAGTC | 3 cut(s) | 278, 496, 1337 |
| PpsI | GAGTC | 3 cut(s) | 278, 496, 1337 |
| PscI | ACATGT | 2 cut(s) | 2002, 2031 |
| PshBI | ATTAAT | 1 cut(s) | 633 |
| Psp124BI | GAGCTC | 1 cut(s) | 1566 |
| Psp6I | CCWGG | 3 cut(s) | 63, 973, 2248 |
| PspFI | CCCAGC | 1 cut(s) | 119 |
| PspGI | CCWGG | 3 cut(s) | 63, 973, 2248 |
| PspN4I | GGNNCC | 7 cut(s) | 128, 693, 1442, 2039, 2094, 2347, 2470 |
| PspOMI | GGGCCC | 1 cut(s) | 2037 |
| PspPI | GGNCC | 6 cut(s) | 433, 2037, 2038, 2093, 2353, 2468 |
| PstI | CTGCAG | 2 cut(s) | 1768, 1921 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2435 |
| PsuI | RGATCY | 3 cut(s) | 721, 1196, 1207 |
| RsaI | GTAC | 6 cut(s) | 57, 128, 544, 594, 1703, 2495 |
| RsaNI | GTAC | 6 cut(s) | 56, 127, 543, 593, 1702, 2494 |
| RseI | CAYNNNNRTG | 2 cut(s) | 395, 2201 |
| SacI | GAGCTC | 1 cut(s) | 1566 |
| SapI | GCTCTTC | 2 cut(s) | 1266, 1554 |
| SaqAI | TTAA | 9 cut(s) | 309, 633, 743, 969, 1119, 1203, 1652, 1839, 2168 |
| SatI | GCNGC | 9 cut(s) | 513, 1051, 1392, 1645, 1764, 1767, 1917, 1920, 1968 |
| Sau3AI | GATC | 9 cut(s) | 109, 213, 721, 769, 893, 1064, 1196, 1207, 2389 |
| Sau96I | GGNCC | 6 cut(s) | 433, 2037, 2038, 2093, 2353, 2468 |
| ScaI | AGTACT | 2 cut(s) | 57, 544 |
| SchI | GAGTC | 3 cut(s) | 278, 497, 1337 |
| ScrFI | CCNGG | 4 cut(s) | 65, 581, 975, 2250 |
| SduI | GDGCHC | 6 cut(s) | 40, 948, 1261, 1277, 1566, 2041 |
| SfaNI | GCATC | 5 cut(s) | 769, 1564, 1759, 2002, 2176 |
| SfcI | CTRYAG | 3 cut(s) | 1764, 1917, 2436 |
| SfuI | TTCGAA | 1 cut(s) | 727 |
| SinI | GGWCC | 3 cut(s) | 433, 2353, 2468 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 395, 2201 |
| SmlI | CTYRAG | 3 cut(s) | 680, 1268, 1565 |
| SmoI | CTYRAG | 3 cut(s) | 680, 1268, 1565 |
| SsiI | CCGC | 5 cut(s) | 919, 1051, 1391, 1435, 2319 |
| SspI | AATATT | 2 cut(s) | 958, 1591 |
| SspMI | CTAG | 6 cut(s) | 378, 891, 1407, 1995, 2097, 2315 |
| SstI | GAGCTC | 1 cut(s) | 1566 |
| StyD4I | CCNGG | 4 cut(s) | 63, 579, 973, 2248 |
| StyI | CCWWGG | 2 cut(s) | 2280, 2356 |
| TaaI | ACNGT | 4 cut(s) | 870, 1057, 1809, 2431 |
| TaiI | ACGT | 3 cut(s) | 280, 1517, 2140 |
| TaqI | TCGA | 6 cut(s) | 108, 280, 495, 727, 750, 2140 |
| TaqII | GACCGA | 1 cut(s) | 1053 |
| TatI | WGTACW | 5 cut(s) | 55, 542, 592, 1701, 2493 |
| TauI | GCSGC | 2 cut(s) | 1053, 1394 |
| TfiI | GAWTC | 6 cut(s) | 70, 455, 656, 1551, 1886, 2100 |
| Tru1I | TTAA | 9 cut(s) | 309, 633, 743, 969, 1119, 1203, 1652, 1839, 2168 |
| Tru9I | TTAA | 9 cut(s) | 309, 633, 743, 969, 1119, 1203, 1652, 1839, 2168 |
| TscAI | CASTG | 4 cut(s) | 1060, 1728, 1861, 2400 |
| TseFI | GTSAC | 3 cut(s) | 380, 912, 1676 |
| TseI | GCWGC | 7 cut(s) | 512, 1644, 1763, 1766, 1916, 1919, 1967 |
| Tsp45I | GTSAC | 3 cut(s) | 380, 912, 1676 |
| TspGWI | ACGGA | 1 cut(s) | 1351 |
| TspRI | CASTG | 4 cut(s) | 1060, 1728, 1861, 2400 |
| VpaK11BI | GGWCC | 3 cut(s) | 433, 2353, 2468 |
| VspI | ATTAAT | 1 cut(s) | 633 |
| XapI | RAATTY | 6 cut(s) | 625, 810, 1218, 1348, 1472, 2209 |
| XbaI | TCTAGA | 2 cut(s) | 890, 1406 |
| XceI | RCATGY | 4 cut(s) | 1094, 1327, 2006, 2035 |
| XmiI | GTMKAC | 1 cut(s) | 1999 |
| XspI | CTAG | 6 cut(s) | 378, 891, 1407, 1995, 2097, 2315 |
| ZrmI | AGTACT | 2 cut(s) | 57, 544 |
| Zsp2I | ATGCAT | 2 cut(s) | 370, 740 |