3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G77680 |
| fragaria_vesca | FvH4_2g26510 |
| malus_domestica | MD08G1168200.v1.1 MD15G1353600.v1.1 |
| prunus_persica | Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 |
| pyrus_communis | pycom08g14370 pycom15g31690 pycom15g31710 |
| rosa_chinensis | RchiOBHm_Chr6g0295321 |
| rosa_laevigata | RLG00000011813 |
| rosa_multiflora | Rmu_sc0012530.1_g000002 Rmu_sc0016917.1_g000001 |
| rosa_roxburghii | Rroxscaffold_171G00441480 Rroxscaffold_7G00172580 |
| rosa_rugosa | Rorug06G0250000 |
| rosa_samantha | Rh6AG363200 Rh6BG371300 Rh6CG377800 Rh6DG363900 |
| rosa_wichuraiana | Rw6G031690 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 780 |
| Acc16I | TGCGCA | 1 cut(s) | 2279 |
| Acc36I | ACCTGC | 1 cut(s) | 965 |
| AccB1I | GGYRCC | 4 cut(s) | 69, 1510, 2075, 2259 |
| AccI | GTMKAC | 2 cut(s) | 2522, 2818 |
| AccII | CGCG | 2 cut(s) | 11, 549 |
| AciI | CCGC | 7 cut(s) | 9, 11, 67, 76, 2210, 2213, 2254 |
| AclI | AACGTT | 2 cut(s) | 334, 1125 |
| AclWI | GGATC | 8 cut(s) | 668, 712, 750, 852, 865, 1548, 1565, 2123 |
| AcoI | YGGCCR | 3 cut(s) | 1867, 1987, 2811 |
| AcsI | RAATTY | 9 cut(s) | 1151, 1463, 1523, 1549, 1615, 2167, 2183, 2291, 2654 |
| AcuI | CTGAAG | 3 cut(s) | 942, 1428, 2066 |
| AcyI | GRCGYC | 1 cut(s) | 70 |
| AfaI | GTAC | 2 cut(s) | 947, 1208 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 81, 402, 854, 1507 |
| AflIII | ACRYGT | 1 cut(s) | 2821 |
| AjnI | CCWGG | 2 cut(s) | 692, 1792 |
| AleI | CACNNNNGTG | 1 cut(s) | 157 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2496, 2528 |
| Alw21I | GWGCWC | 1 cut(s) | 2248 |
| Alw26I | GTCTC | 3 cut(s) | 645, 1502, 2643 |
| Alw44I | GTGCAC | 1 cut(s) | 2244 |
| AlwI | GGATC | 8 cut(s) | 668, 712, 750, 852, 865, 1548, 1565, 2123 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2362 |
| Ama87I | CYCGRG | 1 cut(s) | 1515 |
| AoxI | GGCC | 4 cut(s) | 573, 1867, 1987, 2811 |
| ApaLI | GTGCAC | 1 cut(s) | 2244 |
| ApeKI | GCWGC | 9 cut(s) | 392, 1414, 2440, 2582, 2585, 2699, 2735, 2738, 2786 |
| ApoI | RAATTY | 9 cut(s) | 1151, 1463, 1523, 1549, 1615, 2167, 2183, 2291, 2654 |
| AseI | ATTAAT | 1 cut(s) | 1452 |
| AspLEI | GCGC | 3 cut(s) | 72, 716, 2280 |
| AspS9I | GGNCC | 5 cut(s) | 489, 690, 1252, 1828, 2856 |
| AsuC2I | CCSGG | 1 cut(s) | 411 |
| AsuHPI | GGTGA | 9 cut(s) | 59, 144, 155, 399, 536, 1313, 1364, 1555, 2529 |
| AsuII | TTCGAA | 3 cut(s) | 1440, 1546, 2309 |
| AvaI | CYCGRG | 1 cut(s) | 1515 |
| AvaII | GGWCC | 5 cut(s) | 489, 690, 1252, 1828, 2856 |
| BaeGI | GKGCMC | 2 cut(s) | 2080, 2248 |
| BaeI | ACNNNNGTAYC | 6 cut(s) | 96, 129, 756, 789, 2534, 2567 |
| BalI | TGGCCA | 2 cut(s) | 1989, 2813 |
| BamHI | GGATCC | 1 cut(s) | 857 |
| BanI | GGYRCC | 4 cut(s) | 69, 1510, 2075, 2259 |
| BauI | CACGAG | 1 cut(s) | 1335 |
| BbsI | GAAGAC | 1 cut(s) | 1481 |
| Bbv12I | GWGCWC | 1 cut(s) | 2248 |
| BbvCI | CCTCAGC | 2 cut(s) | 441, 2598 |
| BbvI | GCAGC | 9 cut(s) | 379, 1426, 2452, 2569, 2572, 2686, 2722, 2750, 2773 |
| BccI | CCATC | 8 cut(s) | 38, 352, 584, 1037, 1854, 1922, 2066, 2361 |
| BceAI | ACGGC | 1 cut(s) | 8 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1536, 1570, 2289, 2323 |
| BciT130I | CCWGG | 2 cut(s) | 694, 1794 |
| BciVI | GTATCC | 3 cut(s) | 1655, 2323, 2562 |
| BclI | TGATCA | 1 cut(s) | 928 |
| BcnI | CCSGG | 1 cut(s) | 411 |
| BcoDI | GTCTC | 3 cut(s) | 645, 1502, 2643 |
| BfaI | CTAG | 7 cut(s) | 515, 623, 758, 1533, 1710, 2226, 2579 |
| BfmI | CTRYAG | 4 cut(s) | 600, 768, 2589, 2736 |
| BfoI | RGCGCY | 2 cut(s) | 73, 717 |
| BfuAI | ACCTGC | 1 cut(s) | 965 |
| BfuI | GTATCC | 3 cut(s) | 1655, 2323, 2562 |
| BglII | AGATCT | 1 cut(s) | 2015 |
| BlpI | GCTNAGC | 1 cut(s) | 627 |
| Bme1390I | CCNGG | 3 cut(s) | 411, 694, 1794 |
| Bme18I | GGWCC | 5 cut(s) | 489, 690, 1252, 1828, 2856 |
| BmeT110I | CYCGRG | 1 cut(s) | 1515 |
| BmgT120I | GGNCC | 5 cut(s) | 489, 690, 1252, 1828, 2856 |
| BmiI | GGNNCC | 7 cut(s) | 71, 433, 859, 1512, 2077, 2261, 2858 |
| BmrFI | CCNGG | 3 cut(s) | 411, 694, 1794 |
| BmsI | GCATC | 8 cut(s) | 266, 517, 1093, 1264, 1588, 2578, 2686, 2821 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1971 |
| BpiI | GAAGAC | 1 cut(s) | 1481 |
| BpmI | CTGGAG | 2 cut(s) | 993, 2384 |
| Bpu10I | CCTNAGC | 5 cut(s) | 90, 163, 441, 1914, 2598 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 627 |
| Bpu14I | TTCGAA | 3 cut(s) | 1440, 1546, 2309 |
| BpuEI | CTTGAG | 1 cut(s) | 2369 |
| BpuMI | CCSGG | 1 cut(s) | 411 |
| Bsa29I | ATCGAT | 1 cut(s) | 1858 |
| BsaAI | YACGTR | 1 cut(s) | 879 |
| BsaHI | GRCGYC | 1 cut(s) | 70 |
| BsaJI | CCNNGG | 7 cut(s) | 9, 492, 798, 1232, 1502, 1514, 2097 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 324, 354, 642, 672, 2048, 2053, 2078, 2083 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 81, 402, 854, 1507 |
| Bse118I | RCCGGY | 1 cut(s) | 2547 |
| Bse1I | ACTGG | 7 cut(s) | 491, 734, 1010, 1066, 2367, 2680, 2814 |
| Bse3DI | GCAATG | 3 cut(s) | 2748, 2791, 2803 |
| BseBI | CCWGG | 2 cut(s) | 694, 1794 |
| BseCI | ATCGAT | 1 cut(s) | 1858 |
| BseDI | CCNNGG | 7 cut(s) | 9, 492, 798, 1232, 1502, 1514, 2097 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 81, 402, 854, 1507 |
| BseMI | GCAATG | 3 cut(s) | 2748, 2791, 2803 |
| BseMII | CTCAG | 9 cut(s) | 147, 455, 618, 840, 858, 978, 1928, 1991, 2589 |
| BseNI | ACTGG | 7 cut(s) | 491, 734, 1010, 1066, 2367, 2680, 2814 |
| BseRI | GAGGAG | 3 cut(s) | 429, 2074, 2615 |
| BseSI | GKGCMC | 2 cut(s) | 2080, 2248 |
| BseXI | GCAGC | 9 cut(s) | 379, 1426, 2452, 2569, 2572, 2686, 2722, 2750, 2773 |
| BseYI | CCCAGC | 1 cut(s) | 1841 |
| BsgI | GTGCAG | 3 cut(s) | 714, 1317, 1433 |
| Bsh1236I | CGCG | 2 cut(s) | 11, 549 |
| BshFI | GGCC | 4 cut(s) | 575, 1869, 1989, 2813 |
| BshNI | GGYRCC | 4 cut(s) | 69, 1510, 2075, 2259 |
| BshVI | ATCGAT | 1 cut(s) | 1858 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2248 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1515 |
| BsiSI | CCGG | 3 cut(s) | 411, 1870, 2548 |
| BslFI | GGGAC | 2 cut(s) | 306, 2842 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 81, 402, 854, 1507 |
| BsmAI | GTCTC | 3 cut(s) | 645, 1502, 2643 |
| BsmBI | CGTCTC | 1 cut(s) | 1502 |
| BsmFI | GGGAC | 2 cut(s) | 306, 2842 |
| BsnI | GGCC | 4 cut(s) | 575, 1869, 1989, 2813 |
| BsoBI | CYCGRG | 1 cut(s) | 1515 |
| Bsp119I | TTCGAA | 3 cut(s) | 1440, 1546, 2309 |
| Bsp1286I | GDGCHC | 2 cut(s) | 2080, 2248 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 627 |
| Bsp19I | CCATGG | 2 cut(s) | 492, 1502 |
| BspACI | CCGC | 7 cut(s) | 9, 11, 67, 76, 2210, 2213, 2254 |
| BspANI | GGCC | 4 cut(s) | 575, 1869, 1989, 2813 |
| BspCNI | CTCAG | 9 cut(s) | 148, 454, 619, 841, 859, 979, 1927, 1990, 2590 |
| BspDI | ATCGAT | 1 cut(s) | 1858 |
| BspFNI | CGCG | 2 cut(s) | 11, 549 |
| BspHI | TCATGA | 1 cut(s) | 2415 |
| BspLI | GGNNCC | 7 cut(s) | 71, 433, 859, 1512, 2077, 2261, 2858 |
| BspMAI | CTGCAG | 2 cut(s) | 772, 2740 |
| BspMI | ACCTGC | 1 cut(s) | 965 |
| BspPI | GGATC | 8 cut(s) | 668, 712, 750, 852, 865, 1548, 1565, 2123 |
| BspT104I | TTCGAA | 3 cut(s) | 1440, 1546, 2309 |
| BspT107I | GGYRCC | 4 cut(s) | 69, 1510, 2075, 2259 |
| BsrDI | GCAATG | 3 cut(s) | 2748, 2791, 2803 |
| BsrFI | RCCGGY | 1 cut(s) | 2547 |
| BsrI | ACTGG | 7 cut(s) | 491, 734, 1010, 1066, 2367, 2680, 2814 |
| BssAI | RCCGGY | 1 cut(s) | 2547 |
| BssECI | CCNNGG | 7 cut(s) | 9, 492, 798, 1232, 1502, 1514, 2097 |
| BssNAI | GTATAC | 1 cut(s) | 2523 |
| BssNI | GRCGYC | 1 cut(s) | 70 |
| BssSI | CACGAG | 1 cut(s) | 1335 |
| BssT1I | CCWWGG | 5 cut(s) | 492, 798, 1232, 1502, 2097 |
| Bst1107I | GTATAC | 1 cut(s) | 2523 |
| Bst2BI | CACGAG | 1 cut(s) | 1335 |
| Bst2UI | CCWGG | 2 cut(s) | 694, 1794 |
| Bst4CI | ACNGT | 4 cut(s) | 1628, 1876, 1970, 2243 |
| Bst6I | CTCTTC | 1 cut(s) | 1765 |
| BstACI | GRCGYC | 1 cut(s) | 70 |
| BstBAI | YACGTR | 1 cut(s) | 879 |
| BstBI | TTCGAA | 3 cut(s) | 1440, 1546, 2309 |
| BstC8I | GCNNGC | 6 cut(s) | 712, 1895, 1911, 2282, 2445, 2811 |
| BstDSI | CCRYGG | 3 cut(s) | 9, 492, 1502 |
| BstEII | GGTNACC | 1 cut(s) | 405 |
| BstFNI | CGCG | 2 cut(s) | 11, 549 |
| BstH2I | RGCGCY | 2 cut(s) | 73, 717 |
| BstHHI | GCGC | 3 cut(s) | 72, 716, 2280 |
| BstMAI | GTCTC | 3 cut(s) | 645, 1502, 2643 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 91, 289, 536, 1373, 2266, 2275, 2594, 2732 |
| BstNI | CCWGG | 2 cut(s) | 694, 1794 |
| BstNSI | RCATGY | 3 cut(s) | 1391, 1913, 2825 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1971 |
| BstPI | GGTNACC | 1 cut(s) | 405 |
| BstSCI | CCNGG | 3 cut(s) | 409, 692, 1792 |
| BstSFI | CTRYAG | 4 cut(s) | 600, 768, 2589, 2736 |
| BstSLI | GKGCMC | 2 cut(s) | 2080, 2248 |
| BstUI | CGCG | 2 cut(s) | 11, 549 |
| BstV1I | GCAGC | 9 cut(s) | 379, 1426, 2452, 2569, 2572, 2686, 2722, 2750, 2773 |
| BstV2I | GAAGAC | 1 cut(s) | 1481 |
| BstX2I | RGATCY | 6 cut(s) | 704, 742, 857, 1540, 2015, 2128 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1591 |
| BstYI | RGATCY | 6 cut(s) | 704, 742, 857, 1540, 2015, 2128 |
| BstZ17I | GTATAC | 1 cut(s) | 2523 |
| Bsu15I | ATCGAT | 1 cut(s) | 1858 |
| BsuI | GTATCC | 3 cut(s) | 1655, 2323, 2562 |
| BsuRI | GGCC | 4 cut(s) | 575, 1869, 1989, 2813 |
| BsuTUI | ATCGAT | 1 cut(s) | 1858 |
| BtgI | CCRYGG | 3 cut(s) | 9, 492, 1502 |
| BtgZI | GCGATG | 2 cut(s) | 1721, 2555 |
| BtsI | GCAGTG | 1 cut(s) | 1578 |
| BtsIMutI | CAGTG | 5 cut(s) | 484, 1578, 2248, 2360, 2673 |
| BveI | ACCTGC | 1 cut(s) | 965 |
| Cac8I | GCNNGC | 6 cut(s) | 712, 1895, 1911, 2282, 2445, 2811 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2362 |
| CciI | TCATGA | 1 cut(s) | 2415 |
| CfoI | GCGC | 3 cut(s) | 72, 716, 2280 |
| Cfr10I | RCCGGY | 1 cut(s) | 2547 |
| Cfr13I | GGNCC | 5 cut(s) | 489, 690, 1252, 1828, 2856 |
| Cfr42I | CCGCGG | 1 cut(s) | 12 |
| ClaI | ATCGAT | 1 cut(s) | 1858 |
| CseI | GACGC | 3 cut(s) | 138, 518, 538 |
| Csp6I | GTAC | 2 cut(s) | 946, 1207 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1230, 1265 |
| CviQI | GTAC | 2 cut(s) | 946, 1207 |
| DinI | GGCGCC | 1 cut(s) | 71 |
| DraI | TTTAAA | 1 cut(s) | 535 |
| EaeI | YGGCCR | 3 cut(s) | 1867, 1987, 2811 |
| Eam1104I | CTCTTC | 1 cut(s) | 1765 |
| EarI | CTCTTC | 1 cut(s) | 1765 |
| EciI | GGCGGA | 1 cut(s) | 2228 |
| Eco130I | CCWWGG | 5 cut(s) | 492, 798, 1232, 1502, 2097 |
| Eco147I | AGGCCT | 1 cut(s) | 575 |
| Eco47I | GGWCC | 5 cut(s) | 489, 690, 1252, 1828, 2856 |
| Eco57I | CTGAAG | 3 cut(s) | 942, 1428, 2066 |
| Eco88I | CYCGRG | 1 cut(s) | 1515 |
| Eco91I | GGTNACC | 1 cut(s) | 405 |
| EcoO65I | GGTNACC | 1 cut(s) | 405 |
| EcoRII | CCWGG | 2 cut(s) | 692, 1792 |
| EcoT14I | CCWWGG | 5 cut(s) | 492, 798, 1232, 1502, 2097 |
| EgeI | GGCGCC | 1 cut(s) | 71 |
| EheI | GGCGCC | 1 cut(s) | 71 |
| ErhI | CCWWGG | 5 cut(s) | 492, 798, 1232, 1502, 2097 |
| Esp3I | CGTCTC | 1 cut(s) | 1502 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1347, 1379 |
| FaqI | GGGAC | 2 cut(s) | 306, 2842 |
| FauI | CCCGC | 1 cut(s) | 83 |
| FauNDI | CATATG | 3 cut(s) | 235, 2134, 2852 |
| FbaI | TGATCA | 1 cut(s) | 928 |
| FblI | GTMKAC | 2 cut(s) | 2522, 2818 |
| FspBI | CTAG | 7 cut(s) | 515, 623, 758, 1533, 1710, 2226, 2579 |
| FspI | TGCGCA | 1 cut(s) | 2279 |
| GlaI | GCGC | 3 cut(s) | 71, 715, 2279 |
| GsaI | CCCAGC | 1 cut(s) | 1845 |
| GsuI | CTGGAG | 2 cut(s) | 993, 2384 |
| HaeII | RGCGCY | 2 cut(s) | 73, 717 |
| HaeIII | GGCC | 4 cut(s) | 575, 1869, 1989, 2813 |
| HapII | CCGG | 3 cut(s) | 411, 1870, 2548 |
| HgaI | GACGC | 3 cut(s) | 138, 518, 538 |
| HhaI | GCGC | 3 cut(s) | 72, 716, 2280 |
| Hin1I | GRCGYC | 1 cut(s) | 70 |
| Hin6I | GCGC | 3 cut(s) | 70, 714, 2278 |
| HinP1I | GCGC | 3 cut(s) | 70, 714, 2278 |
| HincII | GTYRAC | 2 cut(s) | 31, 553 |
| HindII | GTYRAC | 2 cut(s) | 31, 553 |
| HindIII | AAGCTT | 2 cut(s) | 1895, 2282 |
| HinfI | GANTC | 9 cut(s) | 226, 302, 414, 890, 962, 1475, 2162, 2393, 2705 |
| HpaII | CCGG | 3 cut(s) | 411, 1870, 2548 |
| HphI | GGTGA | 9 cut(s) | 59, 144, 155, 399, 536, 1313, 1364, 1555, 2529 |
| Hpy166II | GTNNAC | 6 cut(s) | 31, 553, 620, 2246, 2523, 2819 |
| Hpy8I | GTNNAC | 6 cut(s) | 31, 553, 620, 2246, 2523, 2819 |
| Hpy99I | CGWCG | 2 cut(s) | 29, 1101 |
| HpyAV | CCTTC | 7 cut(s) | 641, 1056, 1059, 1584, 2047, 2090, 2152 |
| HpyCH4III | ACNGT | 4 cut(s) | 1628, 1876, 1970, 2243 |
| HpyCH4IV | ACGT | 4 cut(s) | 334, 878, 1096, 1125 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 91, 289, 536, 1373, 2266, 2275, 2594, 2732 |
| HpySE526I | ACGT | 4 cut(s) | 334, 878, 1096, 1125 |
| Hsp92I | GRCGYC | 1 cut(s) | 70 |
| HspAI | GCGC | 3 cut(s) | 70, 714, 2278 |
| KasI | GGCGCC | 1 cut(s) | 69 |
| Ksp22I | TGATCA | 1 cut(s) | 928 |
| KspI | CCGCGG | 1 cut(s) | 12 |
| LmnI | GCTCC | 4 cut(s) | 345, 1372, 1796, 2850 |
| Lsp1109I | GCAGC | 9 cut(s) | 379, 1426, 2452, 2569, 2572, 2686, 2722, 2750, 2773 |
| LweI | GCATC | 8 cut(s) | 266, 517, 1093, 1264, 1588, 2578, 2686, 2821 |
| MaeI | CTAG | 7 cut(s) | 515, 623, 758, 1533, 1710, 2226, 2579 |
| MaeII | ACGT | 4 cut(s) | 334, 878, 1096, 1125 |
| MaeIII | GTNAC | 8 cut(s) | 150, 190, 405, 887, 1674, 1752, 2358, 2495 |
| MfeI | CAATTG | 3 cut(s) | 909, 950, 1311 |
| MflI | RGATCY | 6 cut(s) | 704, 742, 857, 1540, 2015, 2128 |
| MhlI | GDGCHC | 2 cut(s) | 2080, 2248 |
| MlsI | TGGCCA | 2 cut(s) | 1989, 2813 |
| MluNI | TGGCCA | 2 cut(s) | 1989, 2813 |
| Mly113I | GGCGCC | 1 cut(s) | 70 |
| MlyI | GAGTC | 1 cut(s) | 884 |
| MmeI | TCCRAC | 3 cut(s) | 1647, 1983, 2307 |
| Mox20I | TGGCCA | 2 cut(s) | 1989, 2813 |
| MscI | TGGCCA | 2 cut(s) | 1989, 2813 |
| MslI | CAYNNNNRTG | 3 cut(s) | 157, 1214, 2796 |
| Msp20I | TGGCCA | 2 cut(s) | 1989, 2813 |
| MspA1I | CMGCKG | 4 cut(s) | 11, 392, 2210, 2443 |
| MspI | CCGG | 3 cut(s) | 411, 1870, 2548 |
| MspR9I | CCNGG | 3 cut(s) | 411, 694, 1794 |
| MunI | CAATTG | 3 cut(s) | 909, 950, 1311 |
| MvaI | CCWGG | 2 cut(s) | 694, 1794 |
| MvnI | CGCG | 2 cut(s) | 11, 549 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 91, 289, 536, 1373, 2266, 2275, 2594, 2732 |
| NarI | GGCGCC | 1 cut(s) | 70 |
| NciI | CCSGG | 1 cut(s) | 411 |
| NcoI | CCATGG | 2 cut(s) | 492, 1502 |
| NdeI | CATATG | 3 cut(s) | 235, 2134, 2852 |
| NlaIV | GGNNCC | 7 cut(s) | 71, 433, 859, 1512, 2077, 2261, 2858 |
| NmuCI | GTSAC | 5 cut(s) | 150, 405, 887, 2358, 2495 |
| NsbI | TGCGCA | 1 cut(s) | 2279 |
| NspI | RCATGY | 3 cut(s) | 1391, 1913, 2825 |
| NspV | TTCGAA | 3 cut(s) | 1440, 1546, 2309 |
| OliI | CACNNNNGTG | 1 cut(s) | 157 |
| PaeI | GCATGC | 1 cut(s) | 1913 |
| PagI | TCATGA | 1 cut(s) | 2415 |
| PceI | AGGCCT | 1 cut(s) | 575 |
| PciI | ACATGT | 1 cut(s) | 2821 |
| PfeI | GAWTC | 8 cut(s) | 226, 302, 414, 962, 1475, 2162, 2393, 2705 |
| PfoI | TCCNGGA | 1 cut(s) | 1792 |
| PleI | GAGTC | 1 cut(s) | 884 |
| PluTI | GGCGCC | 1 cut(s) | 73 |
| PpsI | GAGTC | 1 cut(s) | 884 |
| Ppu21I | YACGTR | 1 cut(s) | 879 |
| PscI | ACATGT | 1 cut(s) | 2821 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1971 |
| PshBI | ATTAAT | 1 cut(s) | 1452 |
| PsiI | TTATAA | 1 cut(s) | 780 |
| Psp1406I | AACGTT | 2 cut(s) | 334, 1125 |
| Psp6I | CCWGG | 2 cut(s) | 692, 1792 |
| PspEI | GGTNACC | 1 cut(s) | 405 |
| PspFI | CCCAGC | 1 cut(s) | 1841 |
| PspGI | CCWGG | 2 cut(s) | 692, 1792 |
| PspN4I | GGNNCC | 7 cut(s) | 71, 433, 859, 1512, 2077, 2261, 2858 |
| PspPI | GGNCC | 5 cut(s) | 489, 690, 1252, 1828, 2856 |
| PstI | CTGCAG | 2 cut(s) | 772, 2740 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2362 |
| PsuI | RGATCY | 6 cut(s) | 704, 742, 857, 1540, 2015, 2128 |
| PvuII | CAGCTG | 2 cut(s) | 392, 2443 |
| RsaI | GTAC | 2 cut(s) | 947, 1208 |
| RsaNI | GTAC | 2 cut(s) | 946, 1207 |
| RseI | CAYNNNNRTG | 3 cut(s) | 157, 1214, 2796 |
| SacII | CCGCGG | 1 cut(s) | 12 |
| Sau96I | GGNCC | 5 cut(s) | 489, 690, 1252, 1828, 2856 |
| SchI | GAGTC | 1 cut(s) | 884 |
| ScrFI | CCNGG | 3 cut(s) | 411, 694, 1794 |
| SduI | GDGCHC | 2 cut(s) | 2080, 2248 |
| SfaNI | GCATC | 8 cut(s) | 266, 517, 1093, 1264, 1588, 2578, 2686, 2821 |
| SfcI | CTRYAG | 4 cut(s) | 600, 768, 2589, 2736 |
| SfoI | GGCGCC | 1 cut(s) | 71 |
| Sfr303I | CCGCGG | 1 cut(s) | 12 |
| SfuI | TTCGAA | 3 cut(s) | 1440, 1546, 2309 |
| SgrBI | CCGCGG | 1 cut(s) | 12 |
| SinI | GGWCC | 5 cut(s) | 489, 690, 1252, 1828, 2856 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 157, 1214, 2796 |
| SmlI | CTYRAG | 1 cut(s) | 2384 |
| SmoI | CTYRAG | 1 cut(s) | 2384 |
| SphI | GCATGC | 1 cut(s) | 1913 |
| SseBI | AGGCCT | 1 cut(s) | 575 |
| SsiI | CCGC | 7 cut(s) | 9, 11, 67, 76, 2210, 2213, 2254 |
| SspDI | GGCGCC | 1 cut(s) | 69 |
| SspI | AATATT | 3 cut(s) | 1383, 1777, 2410 |
| SspMI | CTAG | 7 cut(s) | 515, 623, 758, 1533, 1710, 2226, 2579 |
| StuI | AGGCCT | 1 cut(s) | 575 |
| StyD4I | CCNGG | 3 cut(s) | 409, 692, 1792 |
| StyI | CCWWGG | 5 cut(s) | 492, 798, 1232, 1502, 2097 |
| TaaI | ACNGT | 4 cut(s) | 1628, 1876, 1970, 2243 |
| TaiI | ACGT | 4 cut(s) | 337, 881, 1099, 1128 |
| TaqI | TCGA | 6 cut(s) | 1014, 1099, 1440, 1546, 1858, 2309 |
| TatI | WGTACW | 1 cut(s) | 1206 |
| TauI | GCSGC | 2 cut(s) | 70, 2213 |
| TfiI | GAWTC | 8 cut(s) | 226, 302, 414, 962, 1475, 2162, 2393, 2705 |
| TscAI | CASTG | 5 cut(s) | 491, 1585, 2248, 2367, 2680 |
| TseFI | GTSAC | 5 cut(s) | 150, 405, 887, 2358, 2495 |
| TseI | GCWGC | 9 cut(s) | 392, 1414, 2440, 2582, 2585, 2699, 2735, 2738, 2786 |
| Tsp45I | GTSAC | 5 cut(s) | 150, 405, 887, 2358, 2495 |
| TspGWI | ACGGA | 1 cut(s) | 2780 |
| TspRI | CASTG | 5 cut(s) | 491, 1585, 2248, 2367, 2680 |
| VneI | GTGCAC | 1 cut(s) | 2244 |
| VpaK11BI | GGWCC | 5 cut(s) | 489, 690, 1252, 1828, 2856 |
| VspI | ATTAAT | 1 cut(s) | 1452 |
| XapI | RAATTY | 9 cut(s) | 1151, 1463, 1523, 1549, 1615, 2167, 2183, 2291, 2654 |
| XbaI | TCTAGA | 4 cut(s) | 514, 757, 1709, 2225 |
| XceI | RCATGY | 3 cut(s) | 1391, 1913, 2825 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 684 |
| XmiI | GTMKAC | 2 cut(s) | 2522, 2818 |
| XspI | CTAG | 7 cut(s) | 515, 623, 758, 1533, 1710, 2226, 2579 |