FvH4_2g26580

E3 ubiquitin-protein ligase RING1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012221
Physical Location & Seq
Forward (+)
21323854 .. 21325988
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26580.t1

Sequence Viewer

Length: 1002 bp
ATGTCTCTGAGCCCTCCTCGTCGACCCAGAAACAACTCCCGCGCTAGACCAATCTATCAGGTCTACTGGTGCTACCAATGCCAAAGACAGGTCAGGATCGCCTCCACACCTGACCCGGTCACCTGCCCGCGCTGCTTCGGGCAGTTCCTCGCCGAAATGGACATCACATTCCGCCCCACTCGCCGCTTCATCGACTACACTGACTTATTCGACACCACCCCAGAAGCGCGTTTGCTAGAAGCACTTTCCCTCATGCTCGACCCGCCCATCCGCGGACTACTTGACCCGGAACCCGAAACCCGACGTCCCCGCCCCAGCAGGCGTAGAAGGCACCGCAGCTTCGATGATGAAATAGAGAGCGGGGACCCGACCCACCCGGAGCTCGGTACCCGGAACCGACCCAGGACGTGGATCATAGTCCGACCCGTCAATGACGACCCCATTCAACCCCTTCCTAGGTCAAGAGTTACTCAACACCCGGGTTTGCCGGTTCCACGTGGAGCGGATCCAGGGAACTATTATTTCGGGTCGGGGCTCCAGGGCCTCATAGAGGAGCTGACACAAAACGACCGACCCGGTCCGCCTCCTGTACCCGAGCCGGCAATCAACTCAATCCCGACCGTTAAGATTATCGAGGACCATTTGAGTAATGACTCGAATTGTCCTATTTGCATGGAGGAGTTTAAGGTTGGTGGTGAGGCTAGGGAGCTCCCATGCAAGCATATATACCATGGTGAGTGCATTGTGCCTTGGCTGAGGCTTCACAATTCTTGCCCGGTTTGCCGGGTGGAGATTCCGGTACCGACTGATGAGGAGTCGGAGGGATCGAATAGTGGGGGAGGGGGGAGGGAAAGGCGGGGCGGGAGGTGGAGCAGGTTCGGGTCTTTGTGGCCGTTTCGGAGGAGATATCGCCGGATTGAACCGCATGGTGGAAATGATGATGGTGAAAGTGCTACTTCTAGAGTTGCTAACCCATGGTGGAGTTCTTGCTCTATTCTTTAG

Protein Analysis

334

Amino Acids

38.04

Weight (kDa)

8.31

Isoelectric Point (pI)

76.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 21 - 53 1.2e-12 zinc-ribbon
zf-RING_2 PF13639 220 - 262 6.8e-15 Ring finger domain
zf-RING_11 PF17123 220 - 248 1.4e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 220 - 261 4e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 221 - 261 2.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 221 - 259 1.7e-06 RING-type zinc-finger
zf-rbx1 PF12678 238 - 262 6.8e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60360
fragaria_vesca FvH4_2g26580 FvH4_2g26580
malus_domestica MD08G1166600.v1.1 MD15G1352600.v1.1
prunus_persica Prupe.1G501100_v2.0.a1
pyrus_communis pycom08g14250
rosa_chinensis RchiOBHm_Chr6g0295451
rosa_laevigata RLG00000011800
rosa_multiflora Rmu_co8384927.1_g000001 Rmu_sc0016348.1_g000002 Rmu_sc0019562.1_g000002
rosa_rugosa Rorug06G0250900 Rorug06G0251000
rosa_samantha Rh6AG364200 Rh6BG372500 Rh6CG378800 Rh6DG365200
rosa_wichuraiana Rw6G031800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 131
AatII GACGTC 1 cut(s) 307
Acc36I ACCTGC 2 cut(s) 131, 864
Acc65I GGTACC 2 cut(s) 386, 799
AccB1I GGYRCC 3 cut(s) 330, 386, 799
AccB7I CCANNNNNTGG 1 cut(s) 408
AccBSI CCGCTC 2 cut(s) 360, 503
AccI GTMKAC 2 cut(s) 22, 63
AccII CGCG 4 cut(s) 42, 130, 229, 273
AclWI GGATC 5 cut(s) 104, 419, 500, 513, 832
AcoI YGGCCR 1 cut(s) 890
AcvI CACGTG 1 cut(s) 497
AcyI GRCGYC 1 cut(s) 304
AfaI GTAC 3 cut(s) 388, 591, 801
AfiI CCNNNNNNNGG 7 cut(s) 88, 272, 383, 408, 456, 550, 929
AgsI TTSAA 2 cut(s) 446, 920
AjiI CACGTC 1 cut(s) 408
AjnI CCWGG 3 cut(s) 401, 508, 537
AluBI AGCT 4 cut(s) 339, 382, 556, 709
AluI AGCT 4 cut(s) 339, 382, 556, 709
Alw21I GWGCWC 2 cut(s) 384, 711
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 5 cut(s) 104, 419, 500, 513, 832
Ama87I CYCGRG 2 cut(s) 478, 593
AoxI GGCC 2 cut(s) 541, 890
ApeKI GCWGC 2 cut(s) 132, 336
Asp718I GGTACC 2 cut(s) 386, 799
AspA2I CCTAGG 1 cut(s) 455
AspLEI GCGC 3 cut(s) 44, 132, 229
AspS9I GGNCC 4 cut(s) 364, 541, 578, 637
AsuC2I CCSGG 9 cut(s) 116, 287, 377, 391, 479, 480, 576, 776, 785
AsuHPI GGTGA 4 cut(s) 112, 707, 746, 956
AvaI CYCGRG 2 cut(s) 478, 593
AvaII GGWCC 3 cut(s) 364, 578, 637
AvrII CCTAGG 1 cut(s) 455
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 3 cut(s) 330, 386, 799
BanII GRGCYC 4 cut(s) 14, 384, 537, 711
BbrPI CACGTG 1 cut(s) 497
Bbv12I GWGCWC 2 cut(s) 384, 711
BbvCI CCTCAGC 1 cut(s) 755
BbvI GCAGC 2 cut(s) 119, 348
BccI CCATC 2 cut(s) 275, 935
BceAI ACGGC 1 cut(s) 877
BciT130I CCWGG 3 cut(s) 403, 510, 539
BcnI CCSGG 9 cut(s) 116, 287, 377, 391, 479, 480, 576, 776, 785
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 5 cut(s) 45, 236, 456, 702, 960
BfuAI ACCTGC 2 cut(s) 131, 864
BisI GCNGC 3 cut(s) 133, 184, 337
BlnI CCTAGG 1 cut(s) 455
BlsI GCNGC 3 cut(s) 134, 185, 338
Bme18I GGWCC 3 cut(s) 364, 578, 637
BmeT110I CYCGRG 2 cut(s) 478, 593
BmgBI CACGTC 1 cut(s) 408
BmgT120I GGNCC 4 cut(s) 364, 541, 578, 637
BplI GAGNNNNNCTC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 521
Bpu10I CCTNAGC 1 cut(s) 755
BpuMI CCSGG 9 cut(s) 116, 287, 377, 391, 479, 480, 576, 776, 785
BsaAI YACGTR 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 304
BsaJI CCNNGG 9 cut(s) 271, 401, 455, 478, 509, 538, 730, 749, 974
BsaWI WCCGGW 1 cut(s) 796
Bsc4I CCNNNNNNNGG 7 cut(s) 88, 272, 383, 408, 456, 550, 929
Bse118I RCCGGY 2 cut(s) 487, 598
Bse1I ACTGG 1 cut(s) 71
BseBI CCWGG 3 cut(s) 403, 510, 539
BseDI CCNNGG 9 cut(s) 271, 401, 455, 478, 509, 538, 730, 749, 974
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 7 cut(s) 88, 272, 383, 408, 456, 550, 929
BseMII CTCAG 1 cut(s) 746
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 5 cut(s) 6, 566, 692, 827, 916
BseXI GCAGC 2 cut(s) 119, 348
BseYI CCCAGC 1 cut(s) 314
Bsh1236I CGCG 4 cut(s) 42, 130, 229, 273
Bsh1285I CGRYCG 2 cut(s) 571, 621
BshFI GGCC 2 cut(s) 543, 892
BshNI GGYRCC 3 cut(s) 330, 386, 799
BsiEI CGRYCG 2 cut(s) 571, 621
BsiHKAI GWGCWC 2 cut(s) 384, 711
BsiHKCI CYCGRG 2 cut(s) 478, 593
BslFI GGGAC 2 cut(s) 291, 377
BslI CCNNNNNNNGG 7 cut(s) 88, 272, 383, 408, 456, 550, 929
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 291, 377
BsnI GGCC 2 cut(s) 543, 892
BsoBI CYCGRG 2 cut(s) 478, 593
Bsp1286I GDGCHC 4 cut(s) 14, 384, 537, 711
Bsp143I GATC 4 cut(s) 96, 411, 505, 824
Bsp19I CCATGG 2 cut(s) 730, 974
BspANI GGCC 2 cut(s) 543, 892
BspCNI CTCAG 1 cut(s) 747
BspFNI CGCG 4 cut(s) 42, 130, 229, 273
BspMI ACCTGC 2 cut(s) 131, 864
BspPI GGATC 5 cut(s) 104, 419, 500, 513, 832
BspT107I GGYRCC 3 cut(s) 330, 386, 799
BsrBI CCGCTC 2 cut(s) 360, 503
BsrFI RCCGGY 2 cut(s) 487, 598
BsrI ACTGG 1 cut(s) 71
BssAI RCCGGY 2 cut(s) 487, 598
BssECI CCNNGG 9 cut(s) 271, 401, 455, 478, 509, 538, 730, 749, 974
BssMI GATC 4 cut(s) 96, 411, 505, 824
BssNI GRCGYC 1 cut(s) 304
BssT1I CCWWGG 4 cut(s) 455, 730, 749, 974
Bst2UI CCWGG 3 cut(s) 403, 510, 539
Bst4CI ACNGT 1 cut(s) 622
BstACI GRCGYC 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 4 cut(s) 128, 320, 600, 719
BstDEI CTNAG 2 cut(s) 8, 755
BstDSI CCRYGG 3 cut(s) 271, 730, 974
BstEII GGTNACC 1 cut(s) 118
BstENI CCTNNNNNAGG 1 cut(s) 548
BstF5I GGATG 1 cut(s) 267
BstFNI CGCG 4 cut(s) 42, 130, 229, 273
BstHHI GCGC 3 cut(s) 44, 132, 229
BstKTI GATC 4 cut(s) 99, 414, 508, 827
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 96, 411, 505, 824
BstMCI CGRYCG 2 cut(s) 571, 621
BstMWI GCNNNNNNNGC 6 cut(s) 78, 132, 180, 262, 328, 780
BstNI CCWGG 3 cut(s) 403, 510, 539
BstPI GGTNACC 1 cut(s) 118
BstUI CGCG 4 cut(s) 42, 130, 229, 273
BstV1I GCAGC 2 cut(s) 119, 348
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
BsuRI GGCC 2 cut(s) 543, 892
BtgI CCRYGG 3 cut(s) 271, 730, 974
BtrI CACGTC 1 cut(s) 408
BtsCI GGATG 1 cut(s) 267
BtsIMutI CAGTG 1 cut(s) 198
BveI ACCTGC 2 cut(s) 131, 864
Cac8I GCNNGC 4 cut(s) 128, 320, 600, 719
CfoI GCGC 3 cut(s) 44, 132, 229
Cfr10I RCCGGY 2 cut(s) 487, 598
Cfr13I GGNCC 4 cut(s) 364, 541, 578, 637
Cfr42I CCGCGG 1 cut(s) 274
Cfr9I CCCGGG 1 cut(s) 478
CpoI CGGWCCG 1 cut(s) 578
Csp6I GTAC 3 cut(s) 387, 590, 800
CspI CGGWCCG 1 cut(s) 578
CviAII CATG 6 cut(s) 253, 673, 714, 731, 926, 975
CviQI GTAC 3 cut(s) 387, 590, 800
DdeI CTNAG 2 cut(s) 8, 755
DpnI GATC 4 cut(s) 98, 413, 507, 826
DpnII GATC 4 cut(s) 96, 411, 505, 824
EaeI YGGCCR 1 cut(s) 890
EciI GGCGGA 2 cut(s) 161, 570
Ecl136II GAGCTC 2 cut(s) 382, 709
Eco130I CCWWGG 4 cut(s) 455, 730, 749, 974
Eco24I GRGCYC 4 cut(s) 14, 384, 537, 711
Eco32I GATATC 1 cut(s) 908
Eco47I GGWCC 3 cut(s) 364, 578, 637
Eco53kI GAGCTC 2 cut(s) 382, 709
Eco72I CACGTG 1 cut(s) 497
Eco88I CYCGRG 2 cut(s) 478, 593
Eco91I GGTNACC 1 cut(s) 118
EcoICRI GAGCTC 2 cut(s) 382, 709
EcoNI CCTNNNNNAGG 1 cut(s) 548
EcoO109I RGGNCCY 2 cut(s) 364, 541
EcoO65I GGTNACC 1 cut(s) 118
EcoRII CCWGG 3 cut(s) 401, 508, 537
EcoRV GATATC 1 cut(s) 908
EcoT14I CCWWGG 4 cut(s) 455, 730, 749, 974
EcoT38I GRGCYC 4 cut(s) 14, 384, 537, 711
ErhI CCWWGG 4 cut(s) 455, 730, 749, 974
FaeI CATG 6 cut(s) 256, 676, 717, 734, 929, 978
FalI AAGNNNNNCTT 2 cut(s) 940, 972
FaqI GGGAC 2 cut(s) 291, 377
FatI CATG 6 cut(s) 252, 672, 713, 730, 925, 974
FauI CCCGC 7 cut(s) 47, 135, 270, 317, 353, 849, 854
FblI GTMKAC 2 cut(s) 22, 63
Fnu4HI GCNGC 3 cut(s) 133, 184, 337
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 4 cut(s) 14, 384, 537, 711
Fsp4HI GCNGC 3 cut(s) 133, 184, 337
FspBI CTAG 5 cut(s) 45, 236, 456, 702, 960
GlaI GCGC 3 cut(s) 43, 131, 228
GluI GCNGC 3 cut(s) 133, 184, 337
GsaI CCCAGC 1 cut(s) 318
GsuI CTGGAG 1 cut(s) 521
HaeIII GGCC 2 cut(s) 543, 892
HhaI GCGC 3 cut(s) 44, 132, 229
Hin1I GRCGYC 1 cut(s) 304
Hin1II CATG 6 cut(s) 256, 676, 717, 734, 929, 978
Hin6I GCGC 3 cut(s) 42, 130, 227
HinP1I GCGC 3 cut(s) 42, 130, 227
HincII GTYRAC 1 cut(s) 23
HindII GTYRAC 1 cut(s) 23
HinfI GANTC 3 cut(s) 653, 793, 815
HphI GGTGA 4 cut(s) 112, 707, 746, 956
Hpy166II GTNNAC 2 cut(s) 23, 64
Hpy188I TCNGA 4 cut(s) 9, 422, 820, 900
Hpy188III TCNNGA 4 cut(s) 94, 462, 616, 960
Hpy8I GTNNAC 2 cut(s) 23, 64
Hpy99I CGWCG 2 cut(s) 24, 306
HpyAV CCTTC 2 cut(s) 321, 461
HpyCH4III ACNGT 1 cut(s) 622
HpyCH4IV ACGT 3 cut(s) 304, 407, 496
HpyCH4V TGCA 3 cut(s) 672, 717, 741
HpyF10VI GCNNNNNNNGC 6 cut(s) 78, 132, 180, 262, 328, 780
HpyF3I CTNAG 2 cut(s) 8, 755
HpySE526I ACGT 3 cut(s) 304, 407, 496
Hsp92I GRCGYC 1 cut(s) 304
Hsp92II CATG 6 cut(s) 256, 676, 717, 734, 929, 978
HspAI GCGC 3 cut(s) 42, 130, 227
KflI GGGWCCC 1 cut(s) 364
KpnI GGTACC 2 cut(s) 390, 803
KroI GCCGGC 1 cut(s) 598
KroNI GCCGGC 1 cut(s) 600
KspI CCGCGG 1 cut(s) 274
Kzo9I GATC 4 cut(s) 96, 411, 505, 824
LmnI GCTCC 7 cut(s) 379, 500, 540, 553, 706, 714, 870
Lsp1109I GCAGC 2 cut(s) 119, 348
MaeI CTAG 5 cut(s) 45, 236, 456, 702, 960
MaeII ACGT 3 cut(s) 304, 407, 496
MaeIII GTNAC 2 cut(s) 118, 466
MalI GATC 4 cut(s) 98, 413, 507, 826
MbiI CCGCTC 2 cut(s) 360, 503
MboI GATC 4 cut(s) 96, 411, 505, 824
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 4 cut(s) 14, 384, 537, 711
MluCI AATT 2 cut(s) 658, 766
MlyI GAGTC 2 cut(s) 647, 824
MmeI TCCRAC 2 cut(s) 445, 798
MroNI GCCGGC 1 cut(s) 598
MseI TTAA 2 cut(s) 624, 684
MspA1I CMGCKG 1 cut(s) 273
MvaI CCWGG 3 cut(s) 403, 510, 539
MvnI CGCG 4 cut(s) 42, 130, 229, 273
MwoI GCNNNNNNNGC 6 cut(s) 78, 132, 180, 262, 328, 780
NaeI GCCGGC 1 cut(s) 600
NciI CCSGG 9 cut(s) 116, 287, 377, 391, 479, 480, 576, 776, 785
NcoI CCATGG 2 cut(s) 730, 974
NdeII GATC 4 cut(s) 96, 411, 505, 824
NgoMIV GCCGGC 1 cut(s) 598
NlaIII CATG 6 cut(s) 256, 676, 717, 734, 929, 978
NmuCI GTSAC 1 cut(s) 118
PaqCI CACCTGC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 824
PdiI GCCGGC 1 cut(s) 600
PfeI GAWTC 1 cut(s) 793
PflFI GACNNNGTC 2 cut(s) 116, 576
PflMI CCANNNNNTGG 1 cut(s) 408
PkrI GCNGC 3 cut(s) 134, 185, 338
PleI GAGTC 2 cut(s) 647, 823
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 2 cut(s) 647, 823
Ppu21I YACGTR 1 cut(s) 497
PpuMI RGGWCCY 1 cut(s) 364
Psp124BI GAGCTC 2 cut(s) 384, 711
Psp5II RGGWCCY 1 cut(s) 364
Psp6I CCWGG 3 cut(s) 401, 508, 537
PspCI CACGTG 1 cut(s) 497
PspEI GGTNACC 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 314
PspGI CCWGG 3 cut(s) 401, 508, 537
PspPI GGNCC 4 cut(s) 364, 541, 578, 637
PspPPI RGGWCCY 1 cut(s) 364
PsuI RGATCY 1 cut(s) 505
PsyI GACNNNGTC 2 cut(s) 116, 576
RsaI GTAC 3 cut(s) 388, 591, 801
RsaNI GTAC 3 cut(s) 387, 590, 800
Rsr2I CGGWCCG 1 cut(s) 578
RsrII CGGWCCG 1 cut(s) 578
SacI GAGCTC 2 cut(s) 384, 711
SacII CCGCGG 1 cut(s) 274
SalI GTCGAC 1 cut(s) 21
SaqAI TTAA 2 cut(s) 624, 684
SatI GCNGC 3 cut(s) 133, 184, 337
Sau3AI GATC 4 cut(s) 96, 411, 505, 824
Sau96I GGNCC 4 cut(s) 364, 541, 578, 637
SchI GAGTC 2 cut(s) 647, 824
SduI GDGCHC 4 cut(s) 14, 384, 537, 711
Sfr303I CCGCGG 1 cut(s) 274
SgrBI CCGCGG 1 cut(s) 274
SinI GGWCC 3 cut(s) 364, 578, 637
SmaI CCCGGG 1 cut(s) 480
Sse9I AATT 2 cut(s) 658, 766
SspMI CTAG 5 cut(s) 45, 236, 456, 702, 960
SstI GAGCTC 2 cut(s) 384, 711
StyI CCWWGG 4 cut(s) 455, 730, 749, 974
TaaI ACNGT 1 cut(s) 622
TaiI ACGT 3 cut(s) 307, 410, 499
TaqI TCGA 8 cut(s) 22, 192, 210, 258, 342, 633, 656, 827
TaqII GACCGA 1 cut(s) 585
TasI AATT 2 cut(s) 658, 766
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 1 cut(s) 793
Tru1I TTAA 2 cut(s) 624, 684
Tru9I TTAA 2 cut(s) 624, 684
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 2 cut(s) 132, 336
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 2 cut(s) 178, 363
TspMI CCCGGG 1 cut(s) 478
TspRI CASTG 1 cut(s) 205
Tth111I GACNNNGTC 2 cut(s) 116, 576
Van91I CCANNNNNTGG 1 cut(s) 408
VpaK11BI GGWCC 3 cut(s) 364, 578, 637
XagI CCTNNNNNAGG 1 cut(s) 548
XbaI TCTAGA 1 cut(s) 959
XmaI CCCGGG 1 cut(s) 478
XmaJI CCTAGG 1 cut(s) 455
XmiI GTMKAC 2 cut(s) 22, 63
XspI CTAG 5 cut(s) 45, 236, 456, 702, 960
ZraI GACGTC 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.