Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012221

Physical Location & Seq

Genomic Coordinates Reverse (-)

42139657 .. 42141426

Gene Structure 1770 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1352600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1062 bp

ATGTCATCGAGTCCAACTCGTTCGAGAGACATTAACATTGACGATGGCACCACATCACCGTACCAGCTCTACTGGTGCTACCACTGCAATCGGACGGTGCGGATTGCCTCCAACAGCCCATCAGAGATAGTCTGCCCTCGTTGTTTCGGGCAGTTTCTCTCCGAGTTTGACATGTCGATTAGGCCTAGACTGCTCGTAGACTACACCAGTTTCGATCCATCCCCAGAAGCTCGTCTGCTCGAAGCACTTTCCCTCATGTTCGACCCGTTCACGAGACCTTTTAACCGTGAATTATTTGACCCAGAGGCTGATCCCGGAGGACGACGCCACCATGAGCATGAGGGCGGGGGAACATGTATAGAACCTGAAGTTCGAGATTTGTGGACAAACAGGAGACGGCGGCGGCGGCGCAACCGTAGCTTTGATGGTGGAGAAAACTGGGAACTGGAACCCGAACCCCGTACGCGAAACCGTCCCAGGACTTGGATTGTTGTCCGACCCGTCGATGATCCTTATAATCCGGTGGGACCAACTTCCCTAACGCCTGAAAACCCAATACTACCTGTTCCACGTGGAGTCGATCTAGGAAACTACTTTTTCGGGCCGGGCCTTCAAGGACTGATCGAGGAATTAACTCAAAACGACAGGCCCGGCCCGCCTCCACTACCCGAATCCGCAATCAACGCCATACCCACTGTGGAAATAAGCGAGGCCCATTTGATCAACGACTCTTGCTGCCCAATTTGCATGGAGGAGTTTAAGGTTGGTGGAGCGGCGAGGGAGTTGCCATGCAATCACATATTCCACAGCGAATGTATTATTCCGTGGCTGAGGCTTCACAACTCTTGCCCGGTCTGCAGGGTCGAGATTCCGGTACCTGGTGGTGTATTGGACGAGTCGGAGGGCTCGAATAGTGGAGGAGAGAGGCGTGGAAGGCGGAGTCGGTTGGGATCATTGTGGCCATCTCGAGCAAGGTATCGTCGAATGGAGCGACAAGGTGGTAATGCTACTTCTACCTCTGGAGCTGGTGATCCATGGTGGAGTTCTTGCACTATTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2 zinc_ribbon_9

Protein Analysis

354

Amino Acids

39.91

Weight (kDa)

5.97

Isoelectric Point (pI)

77.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_ribbon_19	PF14369	22 - 55	9.2e-13	zinc-ribbon
zf-RING_2	PF13639	245 - 287	6.7e-14	Ring finger domain
zf-RING_11	PF17123	246 - 273	1.1e-10	RING-like zinc finger
zf-C3HC4_2	PF13923	246 - 286	9.8e-10	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	246 - 286	8.5e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	246 - 283	3.9e-07	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G60360
fragaria_vesca	FvH4_2g26580 FvH4_2g26580
malus_domestica	MD08G1166600.v1.1 MD15G1352600.v1.1
prunus_persica	Prupe.1G501100_v2.0.a1
pyrus_communis	pycom08g14250
rosa_chinensis	RchiOBHm_Chr6g0295451
rosa_laevigata	RLG00000011800
rosa_multiflora	Rmu_co8384927.1_g000001 Rmu_sc0016348.1_g000002 Rmu_sc0019562.1_g000002
rosa_rugosa	Rorug06G0250900 Rorug06G0251000
rosa_samantha	Rh6AG364200 Rh6BG372500 Rh6CG378800 Rh6DG365200
rosa_wichuraiana	Rw6G031800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	516
Acc65I	GGTACC	1 cut(s)	874
AccB1I	GGYRCC	2 cut(s)	47, 874
AccB7I	CCANNNNNTGG	1 cut(s)	483
AccBSI	CCGCTC	1 cut(s)	773
AccI	GTMKAC	1 cut(s)	198
AccII	CGCG	1 cut(s)	466
AciI	CCGC	9 cut(s)	100, 345, 400, 403, 406, 656, 675, 773, 937
AclWI	GGATC	5 cut(s)	209, 305, 503, 958, 1025
AcoI	YGGCCR	1 cut(s)	959
AcuI	CTGAAG	1 cut(s)	387
AcvI	CACGTG	1 cut(s)	572
AcyI	GRCGYC	1 cut(s)	325
AfaI	GTAC	3 cut(s)	62, 463, 876
AfiI	CCNNNNNNNGG	2 cut(s)	483, 878
AflIII	ACRYGT	2 cut(s)	171, 353
AgsI	TTSAA	1 cut(s)	614
AjnI	CCWGG	2 cut(s)	476, 877
AluBI	AGCT	4 cut(s)	67, 230, 420, 1025
AluI	AGCT	4 cut(s)	67, 230, 420, 1025
Alw26I	GTCTC	3 cut(s)	21, 268, 388
AlwI	GGATC	5 cut(s)	209, 305, 503, 958, 1025
AlwNI	CAGNNNCTG	1 cut(s)	308
Ama87I	CYCGRG	1 cut(s)	966
AoxI	GGCC	7 cut(s)	182, 602, 607, 647, 652, 711, 959
ApeKI	GCWGC	1 cut(s)	735
Asp718I	GGTACC	1 cut(s)	874
AspLEI	GCGC	1 cut(s)	411
AspS9I	GGNCC	6 cut(s)	527, 602, 607, 648, 653, 712
AsuC2I	CCSGG	4 cut(s)	315, 606, 651, 851
AsuHPI	GGTGA	2 cut(s)	48, 1040
AvaI	CYCGRG	1 cut(s)	966
AvaII	GGWCC	1 cut(s)	527
BalI	TGGCCA	1 cut(s)	961
BanI	GGYRCC	2 cut(s)	47, 874
BanII	GRGCYC	1 cut(s)	908
BarI	GAAGNNNNNNTAC	2 cut(s)	994, 1026
BauI	CACGAG	1 cut(s)	271
BbrPI	CACGTG	1 cut(s)	572
BbvCI	CCTCAGC	1 cut(s)	830
BbvI	GCAGC	1 cut(s)	722
BccI	CCATC	5 cut(s)	38, 127, 226, 419, 970
BceAI	ACGGC	1 cut(s)	413
BcgI	CGANNNNNNTGC	2 cut(s)	766, 800
BciT130I	CCWGG	2 cut(s)	478, 879
BclI	TGATCA	1 cut(s)	720
BcnI	CCSGG	4 cut(s)	315, 606, 651, 851
BcoDI	GTCTC	3 cut(s)	21, 268, 388
BfaI	CTAG	2 cut(s)	186, 584
BfmI	CTRYAG	1 cut(s)	856
BisI	GCNGC	5 cut(s)	401, 404, 407, 736, 774
BlsI	GCNGC	5 cut(s)	402, 405, 408, 737, 775
Bme1390I	CCNGG	6 cut(s)	315, 478, 606, 651, 851, 879
Bme18I	GGWCC	1 cut(s)	527
BmeT110I	CYCGRG	1 cut(s)	966
BmgT120I	GGNCC	6 cut(s)	527, 602, 607, 648, 653, 712
BmiI	GGNNCC	4 cut(s)	49, 450, 528, 876
BmrFI	CCNGG	6 cut(s)	315, 478, 606, 651, 851, 879
BmrI	ACTGGG	1 cut(s)	448
BmuI	ACTGGG	1 cut(s)	448
BplI	GAGNNNNNCTC	1 cut(s)	33
BpmI	CTGGAG	1 cut(s)	1041
Bpu10I	CCTNAGC	1 cut(s)	830
BpuMI	CCSGG	4 cut(s)	315, 606, 651, 851
BsaAI	YACGTR	1 cut(s)	572
BsaHI	GRCGYC	1 cut(s)	325
BsaI	GGTCTC	1 cut(s)	268
BsaJI	CCNNGG	3 cut(s)	476, 824, 1034
BsaWI	WCCGGW	2 cut(s)	520, 871
Bsc4I	CCNNNNNNNGG	2 cut(s)	483, 878
Bse1I	ACTGG	4 cut(s)	77, 207, 443, 450
BseBI	CCWGG	2 cut(s)	478, 879
BseDI	CCNNGG	3 cut(s)	476, 824, 1034
BseGI	GGATG	1 cut(s)	218
BseLI	CCNNNNNNNGG	2 cut(s)	483, 878
BseMII	CTCAG	1 cut(s)	821
BseNI	ACTGG	4 cut(s)	77, 207, 443, 450
BseRI	GAGGAG	2 cut(s)	767, 933
BseXI	GCAGC	1 cut(s)	722
Bsh1236I	CGCG	1 cut(s)	466
BshFI	GGCC	7 cut(s)	184, 604, 609, 649, 654, 713, 961
BshNI	GGYRCC	2 cut(s)	47, 874
BsiHKCI	CYCGRG	1 cut(s)	966
BsiSI	CCGG	6 cut(s)	315, 521, 605, 651, 851, 872
BsiWI	CGTACG	1 cut(s)	461
BslFI	GGGAC	2 cut(s)	459, 540
BslI	CCNNNNNNNGG	2 cut(s)	483, 878
BsmAI	GTCTC	3 cut(s)	21, 268, 388
BsmBI	CGTCTC	1 cut(s)	388
BsmFI	GGGAC	2 cut(s)	459, 540
BsnI	GGCC	7 cut(s)	184, 604, 609, 649, 654, 713, 961
Bso31I	GGTCTC	1 cut(s)	268
BsoBI	CYCGRG	1 cut(s)	966
Bsp1286I	GDGCHC	1 cut(s)	908
Bsp143I	GATC	8 cut(s)	214, 310, 508, 580, 621, 720, 950, 1030
Bsp19I	CCATGG	1 cut(s)	1034
BspACI	CCGC	9 cut(s)	100, 345, 400, 403, 406, 656, 675, 773, 937
BspANI	GGCC	7 cut(s)	184, 604, 609, 649, 654, 713, 961
BspCNI	CTCAG	1 cut(s)	822
BspFNI	CGCG	1 cut(s)	466
BspLI	GGNNCC	4 cut(s)	49, 450, 528, 876
BspMAI	CTGCAG	1 cut(s)	860
BspPI	GGATC	5 cut(s)	209, 305, 503, 958, 1025
BspT107I	GGYRCC	2 cut(s)	47, 874
BspTNI	GGTCTC	1 cut(s)	268
BsrBI	CCGCTC	1 cut(s)	773
BsrI	ACTGG	4 cut(s)	77, 207, 443, 450
BssECI	CCNNGG	3 cut(s)	476, 824, 1034
BssMI	GATC	8 cut(s)	214, 310, 508, 580, 621, 720, 950, 1030
BssNI	GRCGYC	1 cut(s)	325
BssSI	CACGAG	1 cut(s)	271
BssT1I	CCWWGG	1 cut(s)	1034
Bst2BI	CACGAG	1 cut(s)	271
Bst2UI	CCWGG	2 cut(s)	478, 879
Bst4CI	ACNGT	6 cut(s)	60, 97, 287, 416, 473, 697
BstACI	GRCGYC	1 cut(s)	325
BstBAI	YACGTR	1 cut(s)	572
BstC8I	GCNNGC	1 cut(s)	656
BstDEI	CTNAG	1 cut(s)	830
BstDSI	CCRYGG	2 cut(s)	824, 1034
BstF5I	GGATG	1 cut(s)	218
BstFNI	CGCG	1 cut(s)	466
BstHHI	GCGC	1 cut(s)	411
BstKTI	GATC	8 cut(s)	217, 313, 511, 583, 624, 723, 953, 1033
BstMAI	GTCTC	3 cut(s)	21, 268, 388
BstMBI	GATC	8 cut(s)	214, 310, 508, 580, 621, 720, 950, 1030
BstMWI	GCNNNNNNNGC	9 cut(s)	84, 190, 406, 417, 655, 683, 744, 855, 934
BstNI	CCWGG	2 cut(s)	478, 879
BstNSI	RCATGY	2 cut(s)	175, 357
BstSCI	CCNGG	6 cut(s)	313, 476, 604, 649, 849, 877
BstSFI	CTRYAG	1 cut(s)	856
BstUI	CGCG	1 cut(s)	466
BstV1I	GCAGC	1 cut(s)	722
BsuRI	GGCC	7 cut(s)	184, 604, 609, 649, 654, 713, 961
BtgI	CCRYGG	2 cut(s)	824, 1034
BtsCI	GGATG	1 cut(s)	218
BtsI	GCAGTG	1 cut(s)	82
BtsIMutI	CAGTG	2 cut(s)	82, 693
Cac8I	GCNNGC	1 cut(s)	656
CaiI	CAGNNNCTG	1 cut(s)	308
CfoI	GCGC	1 cut(s)	411
Cfr13I	GGNCC	6 cut(s)	527, 602, 607, 648, 653, 712
CseI	GACGC	1 cut(s)	333
CsiI	ACCWGGT	1 cut(s)	877
Csp6I	GTAC	3 cut(s)	61, 462, 875
CviAII	CATG	8 cut(s)	172, 256, 332, 338, 354, 748, 789, 1035
CviQI	GTAC	3 cut(s)	61, 462, 875
DdeI	CTNAG	1 cut(s)	830
DpnI	GATC	8 cut(s)	216, 312, 510, 582, 623, 722, 952, 1032
DpnII	GATC	8 cut(s)	214, 310, 508, 580, 621, 720, 950, 1030
EaeI	YGGCCR	1 cut(s)	959
EciI	GGCGGA	1 cut(s)	952
Eco130I	CCWWGG	1 cut(s)	1034
Eco147I	AGGCCT	1 cut(s)	184
Eco24I	GRGCYC	1 cut(s)	908
Eco31I	GGTCTC	1 cut(s)	268
Eco47I	GGWCC	1 cut(s)	527
Eco57I	CTGAAG	1 cut(s)	387
Eco72I	CACGTG	1 cut(s)	572
Eco88I	CYCGRG	1 cut(s)	966
EcoRII	CCWGG	2 cut(s)	476, 877
EcoT14I	CCWWGG	1 cut(s)	1034
EcoT38I	GRGCYC	1 cut(s)	908
ErhI	CCWWGG	1 cut(s)	1034
Esp3I	CGTCTC	1 cut(s)	388
FaeI	CATG	8 cut(s)	175, 259, 335, 341, 357, 751, 792, 1038
FaqI	GGGAC	2 cut(s)	459, 540
FatI	CATG	8 cut(s)	171, 255, 331, 337, 353, 747, 788, 1034
FauI	CCCGC	2 cut(s)	338, 663
FbaI	TGATCA	1 cut(s)	720
FblI	GTMKAC	1 cut(s)	198
Fnu4HI	GCNGC	5 cut(s)	401, 404, 407, 736, 774
FokI	GGATG	1 cut(s)	205
FriOI	GRGCYC	1 cut(s)	908
Fsp4HI	GCNGC	5 cut(s)	401, 404, 407, 736, 774
FspBI	CTAG	2 cut(s)	186, 584
GlaI	GCGC	1 cut(s)	410
GluI	GCNGC	5 cut(s)	401, 404, 407, 736, 774
GsuI	CTGGAG	1 cut(s)	1041
HaeIII	GGCC	7 cut(s)	184, 604, 609, 649, 654, 713, 961
HapII	CCGG	6 cut(s)	315, 521, 605, 651, 851, 872
HgaI	GACGC	1 cut(s)	333
HhaI	GCGC	1 cut(s)	411
Hin1I	GRCGYC	1 cut(s)	325
Hin1II	CATG	8 cut(s)	175, 259, 335, 341, 357, 751, 792, 1038
Hin6I	GCGC	1 cut(s)	409
HinP1I	GCGC	1 cut(s)	409
HinfI	GANTC	7 cut(s)	10, 576, 671, 728, 868, 896, 940
HpaII	CCGG	6 cut(s)	315, 521, 605, 651, 851, 872
HphI	GGTGA	2 cut(s)	48, 1040
Hpy166II	GTNNAC	3 cut(s)	199, 270, 384
Hpy188I	TCNGA	5 cut(s)	93, 124, 163, 497, 901
Hpy188III	TCNNGA	6 cut(s)	24, 271, 374, 865, 966, 1020
Hpy8I	GTNNAC	3 cut(s)	199, 270, 384
Hpy99I	CGWCG	3 cut(s)	327, 506, 984
HpyAV	CCTTC	2 cut(s)	620, 927
HpyCH4III	ACNGT	6 cut(s)	60, 97, 287, 416, 473, 697
HpyCH4IV	ACGT	1 cut(s)	571
HpyCH4V	TGCA	5 cut(s)	87, 747, 792, 858, 1050
HpyF10VI	GCNNNNNNNGC	9 cut(s)	84, 190, 406, 417, 655, 683, 744, 855, 934
HpyF3I	CTNAG	1 cut(s)	830
HpySE526I	ACGT	1 cut(s)	571
Hsp92I	GRCGYC	1 cut(s)	325
Hsp92II	CATG	8 cut(s)	175, 259, 335, 341, 357, 751, 792, 1038
HspAI	GCGC	1 cut(s)	409
KpnI	GGTACC	1 cut(s)	878
Ksp22I	TGATCA	1 cut(s)	720
Kzo9I	GATC	8 cut(s)	214, 310, 508, 580, 621, 720, 950, 1030
LmnI	GCTCC	3 cut(s)	770, 988, 1022
Lsp1109I	GCAGC	1 cut(s)	722
MabI	ACCWGGT	1 cut(s)	877
MaeI	CTAG	2 cut(s)	186, 584
MaeII	ACGT	1 cut(s)	571
MalI	GATC	8 cut(s)	216, 312, 510, 582, 623, 722, 952, 1032
MbiI	CCGCTC	1 cut(s)	773
MboI	GATC	8 cut(s)	214, 310, 508, 580, 621, 720, 950, 1030
MhlI	GDGCHC	1 cut(s)	908
MlsI	TGGCCA	1 cut(s)	961
MluCI	AATT	3 cut(s)	290, 629, 741
MluNI	TGGCCA	1 cut(s)	961
MlyI	GAGTC	5 cut(s)	19, 585, 722, 905, 949
MmeI	TCCRAC	4 cut(s)	38, 135, 520, 879
Mox20I	TGGCCA	1 cut(s)	961
MscI	TGGCCA	1 cut(s)	961
MseI	TTAA	4 cut(s)	33, 282, 632, 759
MslI	CAYNNNNRTG	1 cut(s)	336
Msp20I	TGGCCA	1 cut(s)	961
MspI	CCGG	6 cut(s)	315, 521, 605, 651, 851, 872
MspR9I	CCNGG	6 cut(s)	315, 478, 606, 651, 851, 879
MvaI	CCWGG	2 cut(s)	478, 879
MvnI	CGCG	1 cut(s)	466
MwoI	GCNNNNNNNGC	9 cut(s)	84, 190, 406, 417, 655, 683, 744, 855, 934
NciI	CCSGG	4 cut(s)	315, 606, 651, 851
NcoI	CCATGG	1 cut(s)	1034
NdeII	GATC	8 cut(s)	214, 310, 508, 580, 621, 720, 950, 1030
NlaIII	CATG	8 cut(s)	175, 259, 335, 341, 357, 751, 792, 1038
NlaIV	GGNNCC	4 cut(s)	49, 450, 528, 876
NspI	RCATGY	2 cut(s)	175, 357
PaeR7I	CTCGAG	1 cut(s)	966
PceI	AGGCCT	1 cut(s)	184
PciI	ACATGT	2 cut(s)	171, 353
PcsI	WCGNNNNNNNCGW	2 cut(s)	905, 988
PfeI	GAWTC	2 cut(s)	671, 868
Pfl23II	CGTACG	1 cut(s)	461
PflMI	CCANNNNNTGG	1 cut(s)	483
PfoI	TCCNGGA	1 cut(s)	313
PkrI	GCNGC	5 cut(s)	402, 405, 408, 737, 775
PleI	GAGTC	5 cut(s)	18, 584, 722, 904, 948
PmaCI	CACGTG	1 cut(s)	572
PmlI	CACGTG	1 cut(s)	572
PpsI	GAGTC	5 cut(s)	18, 584, 722, 904, 948
Ppu21I	YACGTR	1 cut(s)	572
PscI	ACATGT	2 cut(s)	171, 353
PsiI	TTATAA	1 cut(s)	516
Psp6I	CCWGG	2 cut(s)	476, 877
PspCI	CACGTG	1 cut(s)	572
PspGI	CCWGG	2 cut(s)	476, 877
PspLI	CGTACG	1 cut(s)	461
PspN4I	GGNNCC	4 cut(s)	49, 450, 528, 876
PspPI	GGNCC	6 cut(s)	527, 602, 607, 648, 653, 712
PstI	CTGCAG	1 cut(s)	860
PstNI	CAGNNNCTG	1 cut(s)	308
RsaI	GTAC	3 cut(s)	62, 463, 876
RsaNI	GTAC	3 cut(s)	61, 462, 875
RseI	CAYNNNNRTG	1 cut(s)	336
SaqAI	TTAA	4 cut(s)	33, 282, 632, 759
SatI	GCNGC	5 cut(s)	401, 404, 407, 736, 774
Sau3AI	GATC	8 cut(s)	214, 310, 508, 580, 621, 720, 950, 1030
Sau96I	GGNCC	6 cut(s)	527, 602, 607, 648, 653, 712
SchI	GAGTC	5 cut(s)	19, 585, 722, 905, 949
ScrFI	CCNGG	6 cut(s)	315, 478, 606, 651, 851, 879
SduI	GDGCHC	1 cut(s)	908
SexAI	ACCWGGT	1 cut(s)	877
SfcI	CTRYAG	1 cut(s)	856
Sfr274I	CTCGAG	1 cut(s)	966
SinI	GGWCC	1 cut(s)	527
SlaI	CTCGAG	1 cut(s)	966
SmiMI	CAYNNNNRTG	1 cut(s)	336
SmlI	CTYRAG	1 cut(s)	966
SmoI	CTYRAG	1 cut(s)	966
Sse9I	AATT	3 cut(s)	290, 629, 741
SseBI	AGGCCT	1 cut(s)	184
SsiI	CCGC	9 cut(s)	100, 345, 400, 403, 406, 656, 675, 773, 937
SspMI	CTAG	2 cut(s)	186, 584
StuI	AGGCCT	1 cut(s)	184
StyD4I	CCNGG	6 cut(s)	313, 476, 604, 649, 849, 877
StyI	CCWWGG	1 cut(s)	1034
TaaI	ACNGT	6 cut(s)	60, 97, 287, 416, 473, 697
TaiI	ACGT	1 cut(s)	574
TasI	AATT	3 cut(s)	290, 629, 741
TauI	GCSGC	4 cut(s)	403, 406, 409, 776
TfiI	GAWTC	2 cut(s)	671, 868
Tru1I	TTAA	4 cut(s)	33, 282, 632, 759
Tru9I	TTAA	4 cut(s)	33, 282, 632, 759
TscAI	CASTG	2 cut(s)	89, 700
TseI	GCWGC	1 cut(s)	735
TspGWI	ACGGA	1 cut(s)	813
TspRI	CASTG	2 cut(s)	89, 700
Van91I	CCANNNNNTGG	1 cut(s)	483
VpaK11BI	GGWCC	1 cut(s)	527
XceI	RCATGY	2 cut(s)	175, 357
XcmI	CCANNNNNNNNNTGG	1 cut(s)	694
XhoI	CTCGAG	1 cut(s)	966
XmiI	GTMKAC	1 cut(s)	198
XspI	CTAG	2 cut(s)	186, 584

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.