Rh6AG364200

E3 ubiquitin-protein ligase RING1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0012221
Physical Location & Seq
Forward (+)
56332140 .. 56333700
1561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG364200.1

Sequence Viewer

Length: 999 bp
ATGTCTCTGAGTCCTCCTCGTCCTGAAAACAACTCTGAGGATAGGCCAATCTACCAGGTCTACTGGTGCTACCAATGCCAAAGGCAGGTCAGGATCGCCTCCACTCCTGACCCAGTCGTCTGCCCGCGCTGTTTCGGGCAGTTCCTCGTCGAAATGGACACCACATTCAGGCCCAGTCGACGCTTCATCGACTTCGAATCCACCCCAGAAGCGCGTTTGCTAGAAGCGCTTTCCCTCATGCTCGACCCGTCCATCCGGCTTTTCGATAGCGGAGCACTCCAGTCGGAACCCGAAACCCGACGTTCCCGCCCAAGCAGGCGTAGAAGAAACCGTAGCTTCGATGATGACATAGAGCGCGGGGACCCGACCCACCCGGAACTCGGTACCCGAAACCCACCCGGGACGTGGATCATAGTACGACCCATCGACGACGATCCGATTCAACCCTTTCGCAGATCAAGAGATAATCAAAACCTGGTTTTACCGATACCACGTGGAGTGGATCCGGGAAACTACTTTGTCGGGTCGGGGCTCCAGGGGCTCATCGAGGAGCTGACCCAAAACGACCGACCCGGACCGCCTCCCTTACCCGAACCGGCAATCAACTCAATCCCAACCGTTAAGATTAGCGAGGCCCATTTGAGCAACGACCCGAATTGTCCTATTTGCATGGAGGAGTTTAAGGTGGGGGGAGAGGCGAGAGAGCTGCCATGCAAGCATATATACCACAATGAATGCATTGTGCCGTGGCTGAGGCTTCACAATTCTTGCCCGGTTTGCCGGGTTGAGATTCCGATTTCGACTGACGAGGAGTCGGACGGTTCGAACAGCGGTGGAGGGAGGAGGGGGCGGCGGGGCATGAGGTGGAGTCGGCTCGGGTCTTTGTGGCCGTTTCGGAGGAGATATCGCCGGATTCAGGTTCAGGGCGGAAATGATGATGATCATGGTGACACTTCGAGAGTTGCCAACCCATGGTGGCATTCTTGCTCTATTCTTTAG

Protein Analysis

332

Amino Acids

38.0

Weight (kDa)

6.67

Isoelectric Point (pI)

74.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 20 - 52 1e-12 zinc-ribbon
zf-RING_2 PF13639 219 - 261 9.6e-15 Ring finger domain
zf-RING_11 PF17123 219 - 247 2e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 219 - 260 4e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 220 - 260 2.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 220 - 258 1.4e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60360
fragaria_vesca FvH4_2g26580 FvH4_2g26580
malus_domestica MD08G1166600.v1.1 MD15G1352600.v1.1
prunus_persica Prupe.1G501100_v2.0.a1
pyrus_communis pycom08g14250
rosa_chinensis RchiOBHm_Chr6g0295451
rosa_laevigata RLG00000011800
rosa_multiflora Rmu_co8384927.1_g000001 Rmu_sc0016348.1_g000002 Rmu_sc0019562.1_g000002
rosa_rugosa Rorug06G0250900 Rorug06G0251000
rosa_samantha Rh6AG364200 Rh6BG372500 Rh6CG378800 Rh6DG365200
rosa_wichuraiana Rw6G031800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
Acc36I ACCTGC 1 cut(s) 76
Acc65I GGTACC 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 972
AccI GTMKAC 2 cut(s) 60, 178
AccII CGCG 3 cut(s) 127, 214, 357
AciI CCGC 9 cut(s) 125, 270, 307, 357, 578, 831, 850, 853, 927
AclWI GGATC 5 cut(s) 101, 416, 428, 497, 510
AcoI YGGCCR 1 cut(s) 887
AcvI CACGTG 1 cut(s) 494
AfaI GTAC 2 cut(s) 385, 417
AfeI AGCGCT 1 cut(s) 228
AfiI CCNNNNNNNGG 6 cut(s) 85, 168, 380, 405, 916, 972
AgsI TTSAA 1 cut(s) 443
AhdI GACNNNNNGTC 1 cut(s) 811
AjiI CACGTC 1 cut(s) 405
AjnI CCWGG 3 cut(s) 54, 474, 534
AluBI AGCT 3 cut(s) 336, 553, 706
AluI AGCT 3 cut(s) 336, 553, 706
Alw21I GWGCWC 1 cut(s) 277
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 5 cut(s) 101, 416, 428, 497, 510
Ama87I CYCGRG 2 cut(s) 398, 875
Aor51HI AGCGCT 1 cut(s) 228
AoxI GGCC 4 cut(s) 44, 170, 633, 887
ApeKI GCWGC 1 cut(s) 706
Asp718I GGTACC 1 cut(s) 383
AspLEI GCGC 4 cut(s) 129, 214, 229, 357
AspS9I GGNCC 4 cut(s) 171, 361, 575, 634
AsuC2I CCSGG 7 cut(s) 374, 399, 400, 507, 573, 773, 782
AsuHPI GGTGA 1 cut(s) 959
AsuII TTCGAA 2 cut(s) 195, 824
AvaI CYCGRG 2 cut(s) 398, 875
AvaII GGWCC 2 cut(s) 361, 575
BamHI GGATCC 1 cut(s) 502
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 2 cut(s) 534, 543
BbrPI CACGTG 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 277
BbvCI CCTCAGC 1 cut(s) 752
BbvI GCAGC 1 cut(s) 693
BccI CCATC 2 cut(s) 260, 431
BceAI ACGGC 2 cut(s) 730, 874
BcgI CGANNNNNNTGC 4 cut(s) 264, 298, 688, 722
BciT130I CCWGG 3 cut(s) 56, 476, 536
BclI TGATCA 1 cut(s) 940
BcnI CCSGG 7 cut(s) 374, 399, 400, 507, 573, 773, 782
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 221
BfoI RGCGCY 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 76
BisI GCNGC 2 cut(s) 707, 851
BlsI GCNGC 2 cut(s) 708, 852
Bme18I GGWCC 2 cut(s) 361, 575
BmeRI GACNNNNNGTC 1 cut(s) 811
BmeT110I CYCGRG 2 cut(s) 398, 875
BmgBI CACGTC 1 cut(s) 405
BmgT120I GGNCC 4 cut(s) 171, 361, 575, 634
BmiI GGNNCC 6 cut(s) 288, 362, 363, 385, 504, 533
BmrI ACTGGG 2 cut(s) 107, 168
BmuI ACTGGG 2 cut(s) 107, 168
BplI GAGNNNNNCTC 1 cut(s) 33
BpmI CTGGAG 2 cut(s) 263, 518
Bpu10I CCTNAGC 1 cut(s) 752
Bpu14I TTCGAA 2 cut(s) 195, 824
BpuMI CCSGG 7 cut(s) 374, 399, 400, 507, 573, 773, 782
BsaAI YACGTR 1 cut(s) 494
BsaBI GATNNNNATC 1 cut(s) 939
BsaJI CCNNGG 4 cut(s) 398, 535, 746, 971
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 168, 380, 405, 916, 972
Bse118I RCCGGY 1 cut(s) 595
Bse1I ACTGG 4 cut(s) 68, 113, 174, 280
Bse8I GATNNNNATC 1 cut(s) 939
BseBI CCWGG 3 cut(s) 56, 476, 536
BseDI CCNNGG 4 cut(s) 398, 535, 746, 971
BseGI GGATG 1 cut(s) 252
BseJI GATNNNNATC 1 cut(s) 939
BseLI CCNNNNNNNGG 6 cut(s) 85, 168, 380, 405, 916, 972
BseMII CTCAG 2 cut(s) 27, 743
BseNI ACTGG 4 cut(s) 68, 113, 174, 280
BseRI GAGGAG 6 cut(s) 6, 563, 689, 824, 856, 913
BseXI GCAGC 1 cut(s) 693
Bsh1236I CGCG 3 cut(s) 127, 214, 357
Bsh1285I CGRYCG 1 cut(s) 568
BshFI GGCC 4 cut(s) 46, 172, 635, 889
BshNI GGYRCC 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 277
BsiHKCI CYCGRG 2 cut(s) 398, 875
BsiSI CCGG 9 cut(s) 256, 374, 399, 506, 573, 596, 773, 781, 910
BslFI GGGAC 2 cut(s) 374, 415
BslI CCNNNNNNNGG 6 cut(s) 85, 168, 380, 405, 916, 972
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 374, 415
BsmI GAATGC 2 cut(s) 740, 979
BsnI GGCC 4 cut(s) 46, 172, 635, 889
BsoBI CYCGRG 2 cut(s) 398, 875
Bsp119I TTCGAA 2 cut(s) 195, 824
Bsp1286I GDGCHC 3 cut(s) 277, 534, 543
Bsp143I GATC 6 cut(s) 93, 408, 433, 455, 502, 940
Bsp19I CCATGG 1 cut(s) 971
BspACI CCGC 9 cut(s) 125, 270, 307, 357, 578, 831, 850, 853, 927
BspANI GGCC 4 cut(s) 46, 172, 635, 889
BspCNI CTCAG 2 cut(s) 28, 744
BspFNI CGCG 3 cut(s) 127, 214, 357
BspLI GGNNCC 6 cut(s) 288, 362, 363, 385, 504, 533
BspMI ACCTGC 1 cut(s) 76
BspPI GGATC 5 cut(s) 101, 416, 428, 497, 510
BspT104I TTCGAA 2 cut(s) 195, 824
BspT107I GGYRCC 1 cut(s) 383
BsrFI RCCGGY 1 cut(s) 595
BsrI ACTGG 4 cut(s) 68, 113, 174, 280
BssAI RCCGGY 1 cut(s) 595
BssECI CCNNGG 4 cut(s) 398, 535, 746, 971
BssMI GATC 6 cut(s) 93, 408, 433, 455, 502, 940
BssT1I CCWWGG 1 cut(s) 971
Bst2UI CCWGG 3 cut(s) 56, 476, 536
Bst4CI ACNGT 3 cut(s) 332, 619, 821
BstBAI YACGTR 1 cut(s) 494
BstBI TTCGAA 2 cut(s) 195, 824
BstC8I GCNNGC 3 cut(s) 125, 317, 716
BstDEI CTNAG 3 cut(s) 8, 36, 752
BstDSI CCRYGG 2 cut(s) 746, 971
BstF5I GGATG 1 cut(s) 252
BstFNI CGCG 3 cut(s) 127, 214, 357
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 4 cut(s) 129, 214, 229, 357
BstKTI GATC 6 cut(s) 96, 411, 436, 458, 505, 943
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 6 cut(s) 93, 408, 433, 455, 502, 940
BstMCI CGRYCG 1 cut(s) 568
BstMWI GCNNNNNNNGC 5 cut(s) 75, 226, 538, 715, 777
BstNI CCWGG 3 cut(s) 56, 476, 536
BstUI CGCG 3 cut(s) 127, 214, 357
BstV1I GCAGC 1 cut(s) 693
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BsuRI GGCC 4 cut(s) 46, 172, 635, 889
BtgI CCRYGG 2 cut(s) 746, 971
BtrI CACGTC 1 cut(s) 405
BtsCI GGATG 1 cut(s) 252
BveI ACCTGC 1 cut(s) 76
Cac8I GCNNGC 3 cut(s) 125, 317, 716
CfoI GCGC 4 cut(s) 129, 214, 229, 357
Cfr10I RCCGGY 1 cut(s) 595
Cfr13I GGNCC 4 cut(s) 171, 361, 575, 634
Cfr9I CCCGGG 1 cut(s) 398
CpoI CGGWCCG 1 cut(s) 575
CseI GACGC 1 cut(s) 189
CsiI ACCWGGT 2 cut(s) 54, 474
Csp6I GTAC 2 cut(s) 384, 416
CspI CGGWCCG 1 cut(s) 575
CviAII CATG 6 cut(s) 238, 670, 711, 859, 944, 972
CviQI GTAC 2 cut(s) 384, 416
DdeI CTNAG 3 cut(s) 8, 36, 752
DpnI GATC 6 cut(s) 95, 410, 435, 457, 504, 942
DpnII GATC 6 cut(s) 93, 408, 433, 455, 502, 940
DrdI GACNNNNNNGTC 1 cut(s) 116
DriI GACNNNNNGTC 1 cut(s) 811
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 887
Eam1105I GACNNNNNGTC 1 cut(s) 811
EciI GGCGGA 1 cut(s) 942
Eco130I CCWWGG 1 cut(s) 971
Eco24I GRGCYC 2 cut(s) 534, 543
Eco32I GATATC 1 cut(s) 905
Eco47I GGWCC 2 cut(s) 361, 575
Eco47III AGCGCT 1 cut(s) 228
Eco72I CACGTG 1 cut(s) 494
Eco88I CYCGRG 2 cut(s) 398, 875
EcoO109I RGGNCCY 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 54, 474, 534
EcoRV GATATC 1 cut(s) 905
EcoT14I CCWWGG 1 cut(s) 971
EcoT22I ATGCAT 1 cut(s) 740
EcoT38I GRGCYC 2 cut(s) 534, 543
ErhI CCWWGG 1 cut(s) 971
FaeI CATG 6 cut(s) 241, 673, 714, 862, 947, 975
FaqI GGGAC 2 cut(s) 374, 415
FatI CATG 6 cut(s) 237, 669, 710, 858, 943, 971
FauI CCCGC 4 cut(s) 132, 314, 350, 846
FbaI TGATCA 1 cut(s) 940
FblI GTMKAC 2 cut(s) 60, 178
Fnu4HI GCNGC 2 cut(s) 707, 851
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 2 cut(s) 534, 543
Fsp4HI GCNGC 2 cut(s) 707, 851
FspBI CTAG 1 cut(s) 221
GlaI GCGC 4 cut(s) 128, 213, 228, 356
GluI GCNGC 2 cut(s) 707, 851
GsuI CTGGAG 2 cut(s) 263, 518
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 4 cut(s) 46, 172, 635, 889
HapII CCGG 9 cut(s) 256, 374, 399, 506, 573, 596, 773, 781, 910
HgaI GACGC 1 cut(s) 189
HhaI GCGC 4 cut(s) 129, 214, 229, 357
Hin1II CATG 6 cut(s) 241, 673, 714, 862, 947, 975
Hin6I GCGC 4 cut(s) 127, 212, 227, 355
HinP1I GCGC 4 cut(s) 127, 212, 227, 355
HincII GTYRAC 1 cut(s) 179
HindII GTYRAC 1 cut(s) 179
HinfI GANTC 7 cut(s) 10, 197, 439, 790, 812, 868, 913
HpaII CCGG 9 cut(s) 256, 374, 399, 506, 573, 596, 773, 781, 910
HphI GGTGA 1 cut(s) 959
Hpy166II GTNNAC 2 cut(s) 61, 179
Hpy188I TCNGA 7 cut(s) 9, 37, 286, 438, 795, 817, 897
Hpy188III TCNNGA 5 cut(s) 23, 91, 107, 459, 957
Hpy8I GTNNAC 2 cut(s) 61, 179
Hpy99I CGWCG 5 cut(s) 152, 183, 303, 431, 434
HpyCH4III ACNGT 3 cut(s) 332, 619, 821
HpyCH4IV ACGT 3 cut(s) 301, 404, 493
HpyCH4V TGCA 3 cut(s) 669, 714, 738
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 226, 538, 715, 777
HpyF3I CTNAG 3 cut(s) 8, 36, 752
HpySE526I ACGT 3 cut(s) 301, 404, 493
Hsp92II CATG 6 cut(s) 241, 673, 714, 862, 947, 975
HspAI GCGC 4 cut(s) 127, 212, 227, 355
KflI GGGWCCC 1 cut(s) 361
KpnI GGTACC 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 940
Kzo9I GATC 6 cut(s) 93, 408, 433, 455, 502, 940
LmnI GCTCC 3 cut(s) 272, 537, 550
Lsp1109I GCAGC 1 cut(s) 693
MabI ACCWGGT 2 cut(s) 54, 474
MaeI CTAG 1 cut(s) 221
MaeII ACGT 3 cut(s) 301, 404, 493
MaeIII GTNAC 1 cut(s) 947
MalI GATC 6 cut(s) 95, 410, 435, 457, 504, 942
MboI GATC 6 cut(s) 93, 408, 433, 455, 502, 940
MboII GAAGA 1 cut(s) 336
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 3 cut(s) 277, 534, 543
MluCI AATT 2 cut(s) 655, 763
MlyI GAGTC 3 cut(s) 19, 821, 877
MmeI TCCRAC 2 cut(s) 264, 795
Mph1103I ATGCAT 1 cut(s) 740
MseI TTAA 2 cut(s) 621, 681
MspA1I CMGCKG 1 cut(s) 831
MspI CCGG 9 cut(s) 256, 374, 399, 506, 573, 596, 773, 781, 910
Mva1269I GAATGC 2 cut(s) 740, 979
MvaI CCWGG 3 cut(s) 56, 476, 536
MvnI CGCG 3 cut(s) 127, 214, 357
MwoI GCNNNNNNNGC 5 cut(s) 75, 226, 538, 715, 777
NciI CCSGG 7 cut(s) 374, 399, 400, 507, 573, 773, 782
NcoI CCATGG 1 cut(s) 971
NdeII GATC 6 cut(s) 93, 408, 433, 455, 502, 940
NlaIII CATG 6 cut(s) 241, 673, 714, 862, 947, 975
NlaIV GGNNCC 6 cut(s) 288, 362, 363, 385, 504, 533
NmuCI GTSAC 1 cut(s) 947
NsiI ATGCAT 1 cut(s) 740
NspV TTCGAA 2 cut(s) 195, 824
PcsI WCGNNNNNNNCGW 1 cut(s) 821
PctI GAATGC 2 cut(s) 740, 979
PfeI GAWTC 4 cut(s) 197, 439, 790, 913
PflFI GACNNNGTC 1 cut(s) 113
PflMI CCANNNNNTGG 1 cut(s) 972
PfoI TCCNGGA 1 cut(s) 505
PkrI GCNGC 2 cut(s) 708, 852
PleI GAGTC 3 cut(s) 18, 820, 876
PmaCI CACGTG 1 cut(s) 494
PmlI CACGTG 1 cut(s) 494
PpsI GAGTC 3 cut(s) 18, 820, 876
Ppu21I YACGTR 1 cut(s) 494
PpuMI RGGWCCY 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 3 cut(s) 54, 474, 534
PspCI CACGTG 1 cut(s) 494
PspGI CCWGG 3 cut(s) 54, 474, 534
PspN4I GGNNCC 6 cut(s) 288, 362, 363, 385, 504, 533
PspPI GGNCC 4 cut(s) 171, 361, 575, 634
PspPPI RGGWCCY 1 cut(s) 361
PsuI RGATCY 1 cut(s) 502
PsyI GACNNNGTC 1 cut(s) 113
RsaI GTAC 2 cut(s) 385, 417
RsaNI GTAC 2 cut(s) 384, 416
Rsr2I CGGWCCG 1 cut(s) 575
RsrII CGGWCCG 1 cut(s) 575
SalI GTCGAC 1 cut(s) 177
SaqAI TTAA 2 cut(s) 621, 681
SatI GCNGC 2 cut(s) 707, 851
Sau3AI GATC 6 cut(s) 93, 408, 433, 455, 502, 940
Sau96I GGNCC 4 cut(s) 171, 361, 575, 634
SchI GAGTC 3 cut(s) 19, 821, 877
SduI GDGCHC 3 cut(s) 277, 534, 543
SexAI ACCWGGT 2 cut(s) 54, 474
SfuI TTCGAA 2 cut(s) 195, 824
SinI GGWCC 2 cut(s) 361, 575
SmaI CCCGGG 1 cut(s) 400
Sse9I AATT 2 cut(s) 655, 763
SsiI CCGC 9 cut(s) 125, 270, 307, 357, 578, 831, 850, 853, 927
SspMI CTAG 1 cut(s) 221
StyI CCWWGG 1 cut(s) 971
TaaI ACNGT 3 cut(s) 332, 619, 821
TaiI ACGT 3 cut(s) 304, 407, 496
TaqII GACCGA 1 cut(s) 582
TasI AATT 2 cut(s) 655, 763
TauI GCSGC 1 cut(s) 853
TfiI GAWTC 4 cut(s) 197, 439, 790, 913
Tru1I TTAA 2 cut(s) 621, 681
Tru9I TTAA 2 cut(s) 621, 681
TseFI GTSAC 1 cut(s) 947
TseI GCWGC 1 cut(s) 706
Tsp45I GTSAC 1 cut(s) 947
TspDTI ATGAA 2 cut(s) 175, 747
TspMI CCCGGG 1 cut(s) 398
Tth111I GACNNNGTC 1 cut(s) 113
Van91I CCANNNNNTGG 1 cut(s) 972
VpaK11BI GGWCC 2 cut(s) 361, 575
XcmI CCANNNNNNNNNTGG 1 cut(s) 402
XmaI CCCGGG 1 cut(s) 398
XmiI GTMKAC 2 cut(s) 60, 178
XspI CTAG 1 cut(s) 221
Zsp2I ATGCAT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.