Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0012221

Physical Location & Seq

Genomic Coordinates Reverse (-)

19735960 .. 19738282

Gene Structure 2323 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1166600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1008 bp

ATGTCTCTGAGTCCACCTCGTTTGAGGGACATTAACAATGACGATGGCACAACACCACTATACGGACTCTACTGGTGCTACCATTGCAATCGGACGGTGAGGACTGCCTCCAACAACCCATCAGAGACAGTCTGCCCTCGTTGTTTCGGGCAGTTTCTGTCGGAGTTTGACATGTCGATTAGACCTAGACTTCTCGTAGACTACACCAGTTTCGATCCATCCCCGGAAGCTCGTCTGCTCGAAGCACTTTCCCTCATGTTCGATCCGCTCATGAGGCCTTTCAACCGTGAATTATATGACCCGGAAGCCGATTCCAGAGGACGTCCATGGTATTGGCGACGCCACCTTGGCCATGAGGGCAGAGGTGCAGGTGTAGAACCTGATGTTCAAGATTTGAGGACAAATAGGAGACGGAGGCGGCGCAACCCTAGCTTTGATGGTAGAGAAAACTGGGAGGTTGAATCTGAGCCCCGCACTCGAAGCCGTCCCAGGAATTTGATTATAGTCCGACCCGTGGAAGACCCTTCTAATCCAGTGGGACCCACTCATAGACCACAAGAAAATCTAGTACTACCCGCTCCACGTGGAGTCGATCTAGGAAACTACTTTTTTGGGCCGGGCCTTCACGGACTGATCGAAGAACTAACCCAAAACGACAGGCCCGGCCCGCCTCCAGTACCCGAAATGGCAATCAACGCAATACCAACTGTGAAAATCAGCGAGGTCCATTTGAGCAATGAGTCTTGTTGCCCAATTTGCATGGAGGAGTTTAAGATTGGTGGAGAGGTGAGGGAGTTGTCATGCAATCACATATACCACAGCGATTGTATTGTTCCGTGGCTGAGGCTCCACAACTCTTGCCCGGTTTGCCGGGTTGAGATTCAGGTATGCGGTGGCGGATTGGTAGAGTTGGGGGGCTCGAATAGTGGCGGAGAGGGGCGGACAAGACGGCGCGTGAGGTGGAGTAGGTTGGGATCTTTGTGGCCATTTCGATCAAGGCATCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2 zinc_ribbon_9

Protein Analysis

336

Amino Acids

38.37

Weight (kDa)

8.28

Isoelectric Point (pI)

69.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_ribbon_19	PF14369	24 - 55	5.1e-12	zinc-ribbon
zf-rbx1	PF12678	247 - 291	6.6e-06	RING-H2 zinc finger domain
zf-RING_2	PF13639	249 - 291	6e-13	Ring finger domain
zf-RING_11	PF17123	250 - 277	1.4e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	250 - 290	1.5e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	250 - 290	6.4e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	250 - 288	3.6e-06	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G60360
fragaria_vesca	FvH4_2g26580 FvH4_2g26580
malus_domestica	MD08G1166600.v1.1 MD15G1352600.v1.1
prunus_persica	Prupe.1G501100_v2.0.a1
pyrus_communis	pycom08g14250
rosa_chinensis	RchiOBHm_Chr6g0295451
rosa_laevigata	RLG00000011800
rosa_multiflora	Rmu_co8384927.1_g000001 Rmu_sc0016348.1_g000002 Rmu_sc0019562.1_g000002
rosa_rugosa	Rorug06G0250900 Rorug06G0251000
rosa_samantha	Rh6AG364200 Rh6BG372500 Rh6CG378800 Rh6DG365200
rosa_wichuraiana	Rw6G031800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	359
AatII	GACGTC	1 cut(s)	325
Acc36I	ACCTGC	1 cut(s)	359
AccBSI	CCGCTC	2 cut(s)	268, 578
AccI	GTMKAC	1 cut(s)	198
AccII	CGCG	1 cut(s)	954
AciI	CCGC	9 cut(s)	266, 418, 472, 576, 668, 891, 897, 930, 940
AclWI	GGATC	3 cut(s)	209, 257, 982
AcoI	YGGCCR	2 cut(s)	349, 983
AcsI	RAATTY	1 cut(s)	493
AcvI	CACGTG	1 cut(s)	584
AcyI	GRCGYC	2 cut(s)	322, 340
AfaI	GTAC	2 cut(s)	570, 678
AfiI	CCNNNNNNNGG	2 cut(s)	62, 514
AflIII	ACRYGT	1 cut(s)	171
AgsI	TTSAA	3 cut(s)	283, 389, 461
AjnI	CCWGG	1 cut(s)	488
AluBI	AGCT	2 cut(s)	230, 432
AluI	AGCT	2 cut(s)	230, 432
Alw26I	GTCTC	3 cut(s)	9, 119, 403
AlwI	GGATC	3 cut(s)	209, 257, 982
AlwNI	CAGNNNCTG	1 cut(s)	157
AoxI	GGCC	7 cut(s)	275, 349, 614, 619, 659, 664, 983
ApoI	RAATTY	1 cut(s)	493
AspLEI	GCGC	2 cut(s)	423, 954
AspS9I	GGNCC	6 cut(s)	539, 614, 619, 660, 665, 724
AsuC2I	CCSGG	6 cut(s)	224, 302, 618, 663, 863, 872
AsuHPI	GGTGA	2 cut(s)	109, 799
AvaII	GGWCC	2 cut(s)	539, 724
BalI	TGGCCA	2 cut(s)	351, 985
BanII	GRGCYC	2 cut(s)	471, 920
BbrPI	CACGTG	1 cut(s)	584
BbsI	GAAGAC	1 cut(s)	525
BbvCI	CCTCAGC	1 cut(s)	842
BccI	CCATC	4 cut(s)	38, 127, 226, 431
BceAI	ACGGC	2 cut(s)	468, 965
BciT130I	CCWGG	1 cut(s)	490
BcnI	CCSGG	6 cut(s)	224, 302, 618, 663, 863, 872
BcoDI	GTCTC	3 cut(s)	9, 119, 403
BfaI	CTAG	4 cut(s)	186, 429, 566, 596
BfuAI	ACCTGC	1 cut(s)	359
BglI	GCCNNNNNGGC	2 cut(s)	348, 357
BisI	GCNGC	1 cut(s)	419
BlsI	GCNGC	1 cut(s)	420
BmcAI	AGTACT	1 cut(s)	570
Bme1390I	CCNGG	7 cut(s)	224, 302, 490, 618, 663, 863, 872
Bme18I	GGWCC	2 cut(s)	539, 724
BmgT120I	GGNCC	6 cut(s)	539, 614, 619, 660, 665, 724
BmiI	GGNNCC	3 cut(s)	540, 541, 848
BmrFI	CCNGG	7 cut(s)	224, 302, 490, 618, 663, 863, 872
BmrI	ACTGGG	1 cut(s)	460
BmuI	ACTGGG	1 cut(s)	460
BpiI	GAAGAC	1 cut(s)	525
BplI	GAGNNNNNCTC	1 cut(s)	33
BpmI	CTGGAG	1 cut(s)	657
Bpu10I	CCTNAGC	1 cut(s)	842
BpuMI	CCSGG	6 cut(s)	224, 302, 618, 663, 863, 872
BsaAI	YACGTR	1 cut(s)	584
BsaHI	GRCGYC	2 cut(s)	322, 340
BsaJI	CCNNGG	6 cut(s)	222, 326, 346, 488, 513, 836
Bsc4I	CCNNNNNNNGG	2 cut(s)	62, 514
Bse1I	ACTGG	5 cut(s)	77, 207, 455, 533, 674
Bse3DI	GCAATG	2 cut(s)	82, 742
BseBI	CCWGG	1 cut(s)	490
BseDI	CCNNGG	6 cut(s)	222, 326, 346, 488, 513, 836
BseGI	GGATG	1 cut(s)	218
BseLI	CCNNNNNNNGG	2 cut(s)	62, 514
BseMI	GCAATG	2 cut(s)	82, 742
BseMII	CTCAG	2 cut(s)	456, 833
BseNI	ACTGG	5 cut(s)	77, 207, 455, 533, 674
BseRI	GAGGAG	1 cut(s)	779
BsgI	GTGCAG	1 cut(s)	387
Bsh1236I	CGCG	1 cut(s)	954
BshFI	GGCC	7 cut(s)	277, 351, 616, 621, 661, 666, 985
BsiSI	CCGG	6 cut(s)	224, 302, 617, 663, 863, 871
BslFI	GGGAC	3 cut(s)	41, 471, 552
BslI	CCNNNNNNNGG	2 cut(s)	62, 514
BsmAI	GTCTC	3 cut(s)	9, 119, 403
BsmBI	CGTCTC	1 cut(s)	403
BsmFI	GGGAC	3 cut(s)	41, 471, 552
BsnI	GGCC	7 cut(s)	277, 351, 616, 621, 661, 666, 985
Bsp1286I	GDGCHC	2 cut(s)	471, 920
Bsp143I	GATC	6 cut(s)	214, 262, 592, 633, 974, 992
Bsp19I	CCATGG	1 cut(s)	326
BspACI	CCGC	9 cut(s)	266, 418, 472, 576, 668, 891, 897, 930, 940
BspANI	GGCC	7 cut(s)	277, 351, 616, 621, 661, 666, 985
BspCNI	CTCAG	2 cut(s)	457, 834
BspFNI	CGCG	1 cut(s)	954
BspHI	TCATGA	1 cut(s)	270
BspLI	GGNNCC	3 cut(s)	540, 541, 848
BspMI	ACCTGC	1 cut(s)	359
BspPI	GGATC	3 cut(s)	209, 257, 982
BsrBI	CCGCTC	2 cut(s)	268, 578
BsrDI	GCAATG	2 cut(s)	82, 742
BsrI	ACTGG	5 cut(s)	77, 207, 455, 533, 674
BssECI	CCNNGG	6 cut(s)	222, 326, 346, 488, 513, 836
BssMI	GATC	6 cut(s)	214, 262, 592, 633, 974, 992
BssNI	GRCGYC	2 cut(s)	322, 340
BssT1I	CCWWGG	2 cut(s)	326, 346
Bst2UI	CCWGG	1 cut(s)	490
Bst4CI	ACNGT	4 cut(s)	97, 130, 287, 709
BstACI	GRCGYC	2 cut(s)	322, 340
BstBAI	YACGTR	1 cut(s)	584
BstC8I	GCNNGC	1 cut(s)	668
BstDEI	CTNAG	3 cut(s)	8, 465, 842
BstDSI	CCRYGG	3 cut(s)	326, 513, 836
BstF5I	GGATG	1 cut(s)	218
BstFNI	CGCG	1 cut(s)	954
BstHHI	GCGC	2 cut(s)	423, 954
BstKTI	GATC	6 cut(s)	217, 265, 595, 636, 977, 995
BstMAI	GTCTC	3 cut(s)	9, 119, 403
BstMBI	GATC	6 cut(s)	214, 262, 592, 633, 974, 992
BstNI	CCWGG	1 cut(s)	490
BstNSI	RCATGY	1 cut(s)	175
BstSCI	CCNGG	7 cut(s)	222, 300, 488, 616, 661, 861, 870
BstUI	CGCG	1 cut(s)	954
BstV2I	GAAGAC	1 cut(s)	525
BstX2I	RGATCY	1 cut(s)	974
BstXI	CCANNNNNNTGG	1 cut(s)	333
BstYI	RGATCY	1 cut(s)	974
BsuRI	GGCC	7 cut(s)	277, 351, 616, 621, 661, 666, 985
BtgI	CCRYGG	3 cut(s)	326, 513, 836
BtsCI	GGATG	1 cut(s)	218
BtsIMutI	CAGTG	1 cut(s)	540
BveI	ACCTGC	1 cut(s)	359
Cac8I	GCNNGC	1 cut(s)	668
CaiI	CAGNNNCTG	1 cut(s)	157
CciI	TCATGA	1 cut(s)	270
CfoI	GCGC	2 cut(s)	423, 954
Cfr13I	GGNCC	6 cut(s)	539, 614, 619, 660, 665, 724
CseI	GACGC	1 cut(s)	348
Csp6I	GTAC	2 cut(s)	569, 677
CspCI	CAANNNNNGTGG	5 cut(s)	38, 806, 839, 841, 874
CviAII	CATG	7 cut(s)	172, 256, 271, 327, 353, 760, 801
CviQI	GTAC	2 cut(s)	569, 677
DdeI	CTNAG	3 cut(s)	8, 465, 842
DpnI	GATC	6 cut(s)	216, 264, 594, 635, 976, 994
DpnII	GATC	6 cut(s)	214, 262, 592, 633, 974, 992
EaeI	YGGCCR	2 cut(s)	349, 983
EciI	GGCGGA	3 cut(s)	912, 945, 955
Eco130I	CCWWGG	2 cut(s)	326, 346
Eco147I	AGGCCT	1 cut(s)	277
Eco24I	GRGCYC	2 cut(s)	471, 920
Eco47I	GGWCC	2 cut(s)	539, 724
Eco72I	CACGTG	1 cut(s)	584
EcoO109I	RGGNCCY	1 cut(s)	539
EcoRII	CCWGG	1 cut(s)	488
EcoT14I	CCWWGG	2 cut(s)	326, 346
EcoT38I	GRGCYC	2 cut(s)	471, 920
ErhI	CCWWGG	2 cut(s)	326, 346
Esp3I	CGTCTC	1 cut(s)	403
FaeI	CATG	7 cut(s)	175, 259, 274, 330, 356, 763, 804
FaqI	GGGAC	3 cut(s)	41, 471, 552
FatI	CATG	7 cut(s)	171, 255, 270, 326, 352, 759, 800
FauI	CCCGC	3 cut(s)	479, 583, 675
FblI	GTMKAC	1 cut(s)	198
Fnu4HI	GCNGC	1 cut(s)	419
FokI	GGATG	1 cut(s)	205
FriOI	GRGCYC	2 cut(s)	471, 920
Fsp4HI	GCNGC	1 cut(s)	419
FspBI	CTAG	4 cut(s)	186, 429, 566, 596
GlaI	GCGC	2 cut(s)	422, 953
GluI	GCNGC	1 cut(s)	419
GsuI	CTGGAG	1 cut(s)	657
HaeIII	GGCC	7 cut(s)	277, 351, 616, 621, 661, 666, 985
HapII	CCGG	6 cut(s)	224, 302, 617, 663, 863, 871
HgaI	GACGC	1 cut(s)	348
HhaI	GCGC	2 cut(s)	423, 954
Hin1I	GRCGYC	2 cut(s)	322, 340
Hin1II	CATG	7 cut(s)	175, 259, 274, 330, 356, 763, 804
Hin6I	GCGC	2 cut(s)	421, 952
HinP1I	GCGC	2 cut(s)	421, 952
HinfI	GANTC	7 cut(s)	10, 66, 311, 461, 588, 740, 880
HpaII	CCGG	6 cut(s)	224, 302, 617, 663, 863, 871
HphI	GGTGA	2 cut(s)	109, 799
Hpy166II	GTNNAC	2 cut(s)	14, 199
Hpy188I	TCNGA	6 cut(s)	9, 93, 124, 163, 466, 509
Hpy188III	TCNNGA	3 cut(s)	271, 315, 389
Hpy8I	GTNNAC	2 cut(s)	14, 199
Hpy99I	CGWCG	1 cut(s)	342
HpyAV	CCTTC	2 cut(s)	534, 632
HpyCH4III	ACNGT	4 cut(s)	97, 130, 287, 709
HpyCH4IV	ACGT	2 cut(s)	322, 583
HpyCH4V	TGCA	4 cut(s)	87, 368, 759, 804
HpyF3I	CTNAG	3 cut(s)	8, 465, 842
HpySE526I	ACGT	2 cut(s)	322, 583
Hsp92I	GRCGYC	2 cut(s)	322, 340
Hsp92II	CATG	7 cut(s)	175, 259, 274, 330, 356, 763, 804
HspAI	GCGC	2 cut(s)	421, 952
KflI	GGGWCCC	1 cut(s)	539
Kzo9I	GATC	6 cut(s)	214, 262, 592, 633, 974, 992
LmnI	GCTCC	2 cut(s)	583, 852
MaeI	CTAG	4 cut(s)	186, 429, 566, 596
MaeII	ACGT	2 cut(s)	322, 583
MalI	GATC	6 cut(s)	216, 264, 594, 635, 976, 994
MbiI	CCGCTC	2 cut(s)	268, 578
MboI	GATC	6 cut(s)	214, 262, 592, 633, 974, 992
MboII	GAAGA	2 cut(s)	530, 650
MflI	RGATCY	1 cut(s)	974
MhlI	GDGCHC	2 cut(s)	471, 920
MlsI	TGGCCA	2 cut(s)	351, 985
MluCI	AATT	3 cut(s)	290, 493, 753
MluNI	TGGCCA	2 cut(s)	351, 985
MlyI	GAGTC	4 cut(s)	19, 60, 597, 749
MmeI	TCCRAC	3 cut(s)	135, 141, 532
Mox20I	TGGCCA	2 cut(s)	351, 985
MscI	TGGCCA	2 cut(s)	351, 985
MseI	TTAA	2 cut(s)	33, 771
Msp20I	TGGCCA	2 cut(s)	351, 985
MspI	CCGG	6 cut(s)	224, 302, 617, 663, 863, 871
MspR9I	CCNGG	7 cut(s)	224, 302, 490, 618, 663, 863, 872
MvaI	CCWGG	1 cut(s)	490
MvnI	CGCG	1 cut(s)	954
NciI	CCSGG	6 cut(s)	224, 302, 618, 663, 863, 872
NcoI	CCATGG	1 cut(s)	326
NdeII	GATC	6 cut(s)	214, 262, 592, 633, 974, 992
NlaIII	CATG	7 cut(s)	175, 259, 274, 330, 356, 763, 804
NlaIV	GGNNCC	3 cut(s)	540, 541, 848
NspI	RCATGY	1 cut(s)	175
PagI	TCATGA	1 cut(s)	270
PaqCI	CACCTGC	1 cut(s)	359
PceI	AGGCCT	1 cut(s)	277
PciI	ACATGT	1 cut(s)	171
PcsI	WCGNNNNNNNCGW	1 cut(s)	633
PfeI	GAWTC	3 cut(s)	311, 461, 880
PkrI	GCNGC	1 cut(s)	420
PleI	GAGTC	4 cut(s)	18, 60, 596, 748
PmaCI	CACGTG	1 cut(s)	584
PmlI	CACGTG	1 cut(s)	584
PpsI	GAGTC	4 cut(s)	18, 60, 596, 748
Ppu21I	YACGTR	1 cut(s)	584
PpuMI	RGGWCCY	1 cut(s)	539
PscI	ACATGT	1 cut(s)	171
Psp5II	RGGWCCY	1 cut(s)	539
Psp6I	CCWGG	1 cut(s)	488
PspCI	CACGTG	1 cut(s)	584
PspGI	CCWGG	1 cut(s)	488
PspN4I	GGNNCC	3 cut(s)	540, 541, 848
PspPI	GGNCC	6 cut(s)	539, 614, 619, 660, 665, 724
PspPPI	RGGWCCY	1 cut(s)	539
PstNI	CAGNNNCTG	1 cut(s)	157
PsuI	RGATCY	1 cut(s)	974
RsaI	GTAC	2 cut(s)	570, 678
RsaNI	GTAC	2 cut(s)	569, 677
SaqAI	TTAA	2 cut(s)	33, 771
SatI	GCNGC	1 cut(s)	419
Sau3AI	GATC	6 cut(s)	214, 262, 592, 633, 974, 992
Sau96I	GGNCC	6 cut(s)	539, 614, 619, 660, 665, 724
ScaI	AGTACT	1 cut(s)	570
SchI	GAGTC	4 cut(s)	19, 60, 597, 749
ScrFI	CCNGG	7 cut(s)	224, 302, 490, 618, 663, 863, 872
SduI	GDGCHC	2 cut(s)	471, 920
SinI	GGWCC	2 cut(s)	539, 724
Sse9I	AATT	3 cut(s)	290, 493, 753
SseBI	AGGCCT	1 cut(s)	277
SsiI	CCGC	9 cut(s)	266, 418, 472, 576, 668, 891, 897, 930, 940
SspMI	CTAG	4 cut(s)	186, 429, 566, 596
StuI	AGGCCT	1 cut(s)	277
StyD4I	CCNGG	7 cut(s)	222, 300, 488, 616, 661, 861, 870
StyI	CCWWGG	2 cut(s)	326, 346
TaaI	ACNGT	4 cut(s)	97, 130, 287, 709
TaiI	ACGT	2 cut(s)	325, 586
TaqI	TCGA	9 cut(s)	176, 213, 240, 261, 478, 591, 636, 920, 991
TasI	AATT	3 cut(s)	290, 493, 753
TatI	WGTACW	1 cut(s)	568
TauI	GCSGC	1 cut(s)	421
TfiI	GAWTC	3 cut(s)	311, 461, 880
Tru1I	TTAA	2 cut(s)	33, 771
Tru9I	TTAA	2 cut(s)	33, 771
TscAI	CASTG	1 cut(s)	540
TspGWI	ACGGA	4 cut(s)	78, 427, 642, 825
TspRI	CASTG	1 cut(s)	540
VpaK11BI	GGWCC	2 cut(s)	539, 724
XapI	RAATTY	1 cut(s)	493
XceI	RCATGY	1 cut(s)	175
XmiI	GTMKAC	1 cut(s)	198
XspI	CTAG	4 cut(s)	186, 429, 566, 596
ZraI	GACGTC	1 cut(s)	323
ZrmI	AGTACT	1 cut(s)	570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.