FvH4_2g29590

Ammonium transporter 3 member 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016764
Physical Location & Seq
Reverse (-)
22919868 .. 22922158
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29590.t1

Sequence Viewer

Length: 1446 bp
ATGAACAACTCCACCTTTGCTCTTCCTCCTGGCCTCATGTATGATGATGCCAGTCCAGAATGGATGAGCAAAGCCGACAACGCATGGCAGCTGACTGCTGCAACTCTGGTTGGCATGCAAAGTGTTCCTGGCCTCATTATCCTATATGGCGGTGCGGTGAAGAAGAAATGGGCTGTGAACTCAGCTTTCATGGCATTCTATGCTTTTGCTTGTGTTCTTGTGTGTTGGGTTGGTTGGGGATATCATATGTCTTTTGGGACTCCACTCTTCACATCAGCTCCATTTTGGGGGAAGGCTGATGTGGCATTGGACCAAAAGTTTCTTTTTACACAGGCTTTTCTTGGGAAGTTCCCTAATGCCACAATGGTGTACTTTCAGTTTGTGTTTGCAGCAATCACTTTGATATTGATTGCTGGAGCTCTTTTGGGAAGGATGAACTTTTATGCTTGGATGTTGTTTGTGCCACTCTGGCTGACATTTTCATACACTGTTGGAGCATATAGTATTTGGAGTCCAAAAGGGTGGCTTGCCTTGAAAGGAATAATTGATTATTCTGGTGGTTATGTCATCCATCTCTCTTCTGGAGTTGCTGGTTTTACAGCAGCCTACTGGGTCGGTCCTCGTTCAACCAAGGACAGGGAAAGGTTCCCCCCAAACAACATTCTACTTATGTTGACTGGAGCAGGGTTGCTCTGGATGGGATGGACAGGGTTCAATGGAGGAGACCCTTATGTAGTCAGTATGGATGCTTCATTGGCTGTCTTGAACACTCATGTCTGCACCGCTACCAGCTTGCTCACTTGGCTAGCTCTTGATGTCATCTTTTTCCGCAAGCCTTCTGTCATCGGTGCAGTTCAAGGCATGATCACTGGTTTGGTTTGCATCACCCCAGCTGCTGGAGTTGTGCAAGGATGGGCAGCCATAATAATGGGGATTCTCTCTGGCTCAATCCCTTGGTATACCATGATGGTTGTCCACAAGAAATCTATGCTTCTTCAGAAAGTTGATGACACAATGGCTGTCCTACACACTCATGCCATTGCCGGAGCCCTTGGTGGACTCCTCACCGGTCTCTTTGCTCATCCAAAGCTCAATTACTTGTTCTATAGCACCTATGGAACATATGTTGGTCTATTTTATGGTATTGACATGCGAAGATTCAAGGCCGGAGCTCGTCAAGTAGGAATTCAACTTCTCGGAGTACTCTTCGTTGTTACACTAAATGTAGTGGTGACAAGCCTTGTATGCCTTTTGGTACAACTCATTGTGCCTCTGAGAATGTCTGAAGAGGATATGGAGATTGGTGATGAAGCAGCTCATGGTGAAGAGGCTTATGCTATCTGGGGTCAAGGTGAAAAGCTAGACAATTCAAGGCATGGGGCATATGATATTGAATCATCTGCAAAGGGTAGAGCCAGGTCTGCTGGAAATATTGAGATGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

482

Amino Acids

52.4

Weight (kDa)

8.14

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 28 - 445 9.6e-81 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016764)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29590
malus_domestica MD08G1145400.v1.1 MD15G1121000.v1.1
prunus_persica Prupe.1G473600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298571
rosa_laevigata RLG00000011503
rosa_multiflora Rmu_sc0004500.1_g000011
rosa_roxburghii Rroxscaffold_7G00169530
rosa_rugosa Rorug06G0281000
rosa_samantha Rh6AG392600 Rh6BG400400 Rh6CG406300 Rh6DG393000
rosa_wichuraiana Rw6G034320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 896
AccI GTMKAC 1 cut(s) 959
AciI CCGC 4 cut(s) 150, 155, 783, 829
AcsI RAATTY 1 cut(s) 1185
AcuI CTGAAG 2 cut(s) 980, 1305
AfaI GTAC 3 cut(s) 371, 1203, 1257
AfiI CCNNNNNNNGG 3 cut(s) 287, 636, 896
AgeI ACCGGT 1 cut(s) 1067
AgsI TTSAA 9 cut(s) 535, 627, 715, 766, 857, 1162, 1190, 1371, 1394
AjnI CCWGG 3 cut(s) 28, 127, 1415
AleI CACNNNNGTG 1 cut(s) 365
Alw21I GWGCWC 2 cut(s) 421, 1174
Alw26I GTCTC 2 cut(s) 717, 1076
AlwNI CAGNNNCTG 1 cut(s) 896
AoxI GGCC 3 cut(s) 31, 130, 1164
ApeKI GCWGC 7 cut(s) 88, 98, 389, 602, 893, 917, 1313
ApoI RAATTY 1 cut(s) 1185
AsiGI ACCGGT 1 cut(s) 1067
AspS9I GGNCC 2 cut(s) 310, 617
AsuHPI GGTGA 7 cut(s) 169, 877, 1057, 1243, 1316, 1334, 1364
AsuNHI GCTAGC 1 cut(s) 805
AvaII GGWCC 2 cut(s) 310, 617
BanII GRGCYC 3 cut(s) 421, 1051, 1174
BarI GAAGNNNNNNTAC 2 cut(s) 733, 765
Bbv12I GWGCWC 2 cut(s) 421, 1174
BbvI GCAGC 7 cut(s) 85, 100, 401, 614, 880, 929, 1325
BccI CCATC 6 cut(s) 579, 691, 696, 906, 961, 1432
BciT130I CCWGG 3 cut(s) 30, 129, 1417
BclI TGATCA 1 cut(s) 864
BcoDI GTCTC 2 cut(s) 717, 1076
BfaI CTAG 2 cut(s) 806, 1361
BfmI CTRYAG 1 cut(s) 1105
BglI GCCNNNNNGGC 1 cut(s) 469
BisI GCNGC 7 cut(s) 89, 99, 390, 603, 894, 918, 1314
BlsI GCNGC 7 cut(s) 90, 100, 391, 604, 895, 919, 1315
BmcAI AGTACT 1 cut(s) 1203
Bme1390I CCNGG 3 cut(s) 30, 129, 1417
Bme18I GGWCC 2 cut(s) 310, 617
BmgT120I GGNCC 2 cut(s) 310, 617
BmiI GGNNCC 2 cut(s) 647, 1048
BmrFI CCNGG 3 cut(s) 30, 129, 1417
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 3 cut(s) 37, 736, 891
BmtI GCTAGC 1 cut(s) 809
BmuI ACTGGG 1 cut(s) 619
BpmI CTGGAG 4 cut(s) 435, 603, 699, 918
BsaI GGTCTC 2 cut(s) 717, 1076
BsaJI CCNNGG 3 cut(s) 630, 953, 1051
BsaWI WCCGGW 1 cut(s) 1067
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 636, 896
Bse118I RCCGGY 1 cut(s) 1067
Bse1I ACTGG 4 cut(s) 51, 614, 682, 874
Bse3DI GCAATG 1 cut(s) 1038
BseBI CCWGG 3 cut(s) 30, 129, 1417
BseDI CCNNGG 3 cut(s) 630, 953, 1051
BseGI GGATG 9 cut(s) 69, 438, 456, 567, 702, 707, 751, 917, 1081
BseLI CCNNNNNNNGG 3 cut(s) 287, 636, 896
BseMI GCAATG 1 cut(s) 1038
BseMII CTCAG 2 cut(s) 195, 1265
BseNI ACTGG 4 cut(s) 51, 614, 682, 874
BseRI GAGGAG 2 cut(s) 735, 1052
BseXI GCAGC 7 cut(s) 85, 100, 401, 614, 880, 929, 1325
BseYI CCCAGC 1 cut(s) 889
BsgI GTGCAG 2 cut(s) 763, 870
BshFI GGCC 3 cut(s) 33, 132, 1166
BshTI ACCGGT 1 cut(s) 1067
BsiHKAI GWGCWC 2 cut(s) 421, 1174
BsiSI CCGG 3 cut(s) 1044, 1068, 1167
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 3 cut(s) 287, 636, 896
BsmAI GTCTC 2 cut(s) 717, 1076
BsmFI GGGAC 1 cut(s) 271
BsmI GAATGC 1 cut(s) 194
BsnI GGCC 3 cut(s) 33, 132, 1166
Bso31I GGTCTC 2 cut(s) 717, 1076
Bsp1286I GDGCHC 3 cut(s) 421, 1051, 1174
Bsp143I GATC 1 cut(s) 864
BspACI CCGC 4 cut(s) 150, 155, 783, 829
BspANI GGCC 3 cut(s) 33, 132, 1166
BspCNI CTCAG 2 cut(s) 194, 1266
BspLI GGNNCC 2 cut(s) 647, 1048
BspOI GCTAGC 1 cut(s) 809
BspQI GCTCTTC 1 cut(s) 27
BspTNI GGTCTC 2 cut(s) 717, 1076
BsrDI GCAATG 1 cut(s) 1038
BsrFI RCCGGY 1 cut(s) 1067
BsrI ACTGG 4 cut(s) 51, 614, 682, 874
BssAI RCCGGY 1 cut(s) 1067
BssECI CCNNGG 3 cut(s) 630, 953, 1051
BssMI GATC 1 cut(s) 864
BssNAI GTATAC 1 cut(s) 960
BssT1I CCWWGG 3 cut(s) 630, 953, 1051
Bst1107I GTATAC 1 cut(s) 960
Bst2UI CCWGG 3 cut(s) 30, 129, 1417
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 6 cut(s) 27, 272, 583, 1211, 1281, 1320
BstAPI GCANNNNNTGC 1 cut(s) 200
BstC8I GCNNGC 5 cut(s) 116, 528, 794, 807, 833
BstDEI CTNAG 2 cut(s) 181, 1274
BstF5I GGATG 9 cut(s) 69, 438, 456, 567, 702, 707, 751, 917, 1081
BstKTI GATC 1 cut(s) 867
BstMAI GTCTC 2 cut(s) 717, 1076
BstMBI GATC 1 cut(s) 864
BstMWI GCNNNNNNNGC 9 cut(s) 80, 191, 200, 302, 469, 755, 802, 1245, 1421
BstNI CCWGG 3 cut(s) 30, 129, 1417
BstNSI RCATGY 2 cut(s) 118, 1153
BstSCI CCNGG 3 cut(s) 28, 127, 1415
BstSFI CTRYAG 1 cut(s) 1105
BstV1I GCAGC 7 cut(s) 85, 100, 401, 614, 880, 929, 1325
BstXI CCANNNNNNTGG 2 cut(s) 522, 928
BstZ17I GTATAC 1 cut(s) 960
BsuRI GGCC 3 cut(s) 33, 132, 1166
BtsCI GGATG 9 cut(s) 69, 438, 456, 567, 702, 707, 751, 917, 1081
BtsIMutI CAGTG 2 cut(s) 486, 867
Cac8I GCNNGC 5 cut(s) 116, 528, 794, 807, 833
CaiI CAGNNNCTG 1 cut(s) 896
Cfr10I RCCGGY 1 cut(s) 1067
Cfr13I GGNCC 2 cut(s) 310, 617
Csp6I GTAC 3 cut(s) 370, 1202, 1256
CspAI ACCGGT 1 cut(s) 1067
CviQI GTAC 3 cut(s) 370, 1202, 1256
DdeI CTNAG 2 cut(s) 181, 1274
DpnI GATC 1 cut(s) 866
DpnII GATC 1 cut(s) 864
Eam1104I CTCTTC 6 cut(s) 27, 272, 583, 1211, 1281, 1320
EarI CTCTTC 6 cut(s) 27, 272, 583, 1211, 1281, 1320
Ecl136II GAGCTC 2 cut(s) 419, 1172
Eco130I CCWWGG 3 cut(s) 630, 953, 1051
Eco24I GRGCYC 3 cut(s) 421, 1051, 1174
Eco31I GGTCTC 2 cut(s) 717, 1076
Eco32I GATATC 1 cut(s) 242
Eco47I GGWCC 2 cut(s) 310, 617
Eco53kI GAGCTC 2 cut(s) 419, 1172
Eco57I CTGAAG 2 cut(s) 980, 1305
EcoICRI GAGCTC 2 cut(s) 419, 1172
EcoRI GAATTC 1 cut(s) 1185
EcoRII CCWGG 3 cut(s) 28, 127, 1415
EcoRV GATATC 1 cut(s) 242
EcoT14I CCWWGG 3 cut(s) 630, 953, 1051
EcoT38I GRGCYC 3 cut(s) 421, 1051, 1174
ErhI CCWWGG 3 cut(s) 630, 953, 1051
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 1 cut(s) 271
FauNDI CATATG 3 cut(s) 246, 1123, 1384
FbaI TGATCA 1 cut(s) 864
FblI GTMKAC 1 cut(s) 959
Fnu4HI GCNGC 7 cut(s) 89, 99, 390, 603, 894, 918, 1314
FokI GGATG 9 cut(s) 76, 445, 463, 554, 709, 714, 758, 924, 1068
FriOI GRGCYC 3 cut(s) 421, 1051, 1174
Fsp4HI GCNGC 7 cut(s) 89, 99, 390, 603, 894, 918, 1314
FspBI CTAG 2 cut(s) 806, 1361
GluI GCNGC 7 cut(s) 89, 99, 390, 603, 894, 918, 1314
GsaI CCCAGC 1 cut(s) 893
GsuI CTGGAG 4 cut(s) 435, 603, 699, 918
HaeIII GGCC 3 cut(s) 33, 132, 1166
HapII CCGG 3 cut(s) 1044, 1068, 1167
HincII GTYRAC 1 cut(s) 675
HindII GTYRAC 1 cut(s) 675
HinfI GANTC 6 cut(s) 259, 511, 934, 1059, 1158, 1394
HpaII CCGG 3 cut(s) 1044, 1068, 1167
HphI GGTGA 7 cut(s) 169, 877, 1057, 1243, 1316, 1334, 1364
Hpy166II GTNNAC 6 cut(s) 178, 370, 675, 960, 976, 1058
Hpy188I TCNGA 4 cut(s) 999, 1199, 1275, 1285
Hpy188III TCNNGA 5 cut(s) 56, 582, 694, 763, 812
Hpy8I GTNNAC 6 cut(s) 178, 370, 675, 960, 976, 1058
HpyAV CCTTC 3 cut(s) 286, 423, 846
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 8 cut(s) 101, 118, 389, 780, 851, 882, 907, 1403
HpyF10VI GCNNNNNNNGC 9 cut(s) 80, 191, 200, 302, 469, 755, 802, 1245, 1421
HpyF3I CTNAG 2 cut(s) 181, 1274
Ksp22I TGATCA 1 cut(s) 864
Kzo9I GATC 1 cut(s) 864
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 6 cut(s) 283, 416, 494, 680, 1046, 1169
Lsp1109I GCAGC 7 cut(s) 85, 100, 401, 614, 880, 929, 1325
LweI GCATC 3 cut(s) 37, 736, 891
MaeI CTAG 2 cut(s) 806, 1361
MaeIII GTNAC 2 cut(s) 1213, 1231
MalI GATC 1 cut(s) 866
MboI GATC 1 cut(s) 864
MhlI GDGCHC 3 cut(s) 421, 1051, 1174
MluCI AATT 4 cut(s) 543, 1093, 1185, 1366
MlyI GAGTC 3 cut(s) 253, 520, 1053
MmeI TCCRAC 1 cut(s) 472
MnlI CCTC 9 cut(s) 36, 44, 143, 630, 713, 1073, 1281, 1282, 1321
MslI CAYNNNNRTG 3 cut(s) 365, 926, 1032
MspA1I CMGCKG 2 cut(s) 91, 893
MspI CCGG 3 cut(s) 1044, 1068, 1167
MspR9I CCNGG 3 cut(s) 30, 129, 1417
Mva1269I GAATGC 1 cut(s) 194
MvaI CCWGG 3 cut(s) 30, 129, 1417
MwoI GCNNNNNNNGC 9 cut(s) 80, 191, 200, 302, 469, 755, 802, 1245, 1421
NdeI CATATG 3 cut(s) 246, 1123, 1384
NdeII GATC 1 cut(s) 864
NheI GCTAGC 1 cut(s) 805
NlaIV GGNNCC 2 cut(s) 647, 1048
NmuCI GTSAC 1 cut(s) 1231
NspI RCATGY 2 cut(s) 118, 1153
OliI CACNNNNGTG 1 cut(s) 365
PaeI GCATGC 1 cut(s) 118
PciSI GCTCTTC 1 cut(s) 27
PctI GAATGC 1 cut(s) 194
PfeI GAWTC 3 cut(s) 934, 1158, 1394
PflMI CCANNNNNTGG 1 cut(s) 896
PinAI ACCGGT 1 cut(s) 1067
PkrI GCNGC 7 cut(s) 90, 100, 391, 604, 895, 919, 1315
PleI GAGTC 3 cut(s) 253, 519, 1053
PpsI GAGTC 3 cut(s) 253, 519, 1053
Psp124BI GAGCTC 2 cut(s) 421, 1174
Psp6I CCWGG 3 cut(s) 28, 127, 1415
PspFI CCCAGC 1 cut(s) 889
PspGI CCWGG 3 cut(s) 28, 127, 1415
PspN4I GGNNCC 2 cut(s) 647, 1048
PspPI GGNCC 2 cut(s) 310, 617
PstNI CAGNNNCTG 1 cut(s) 896
PvuII CAGCTG 2 cut(s) 91, 893
RsaI GTAC 3 cut(s) 371, 1203, 1257
RsaNI GTAC 3 cut(s) 370, 1202, 1256
RseI CAYNNNNRTG 3 cut(s) 365, 926, 1032
SacI GAGCTC 2 cut(s) 421, 1174
SapI GCTCTTC 1 cut(s) 27
SatI GCNGC 7 cut(s) 89, 99, 390, 603, 894, 918, 1314
Sau3AI GATC 1 cut(s) 864
Sau96I GGNCC 2 cut(s) 310, 617
ScaI AGTACT 1 cut(s) 1203
SchI GAGTC 3 cut(s) 253, 520, 1053
ScrFI CCNGG 3 cut(s) 30, 129, 1417
SduI GDGCHC 3 cut(s) 421, 1051, 1174
SfaNI GCATC 3 cut(s) 37, 736, 891
SfcI CTRYAG 1 cut(s) 1105
SinI GGWCC 2 cut(s) 310, 617
SmiMI CAYNNNNRTG 3 cut(s) 365, 926, 1032
SphI GCATGC 1 cut(s) 118
Sse9I AATT 4 cut(s) 543, 1093, 1185, 1366
SsiI CCGC 4 cut(s) 150, 155, 783, 829
SspI AATATT 1 cut(s) 1432
SspMI CTAG 2 cut(s) 806, 1361
SstI GAGCTC 2 cut(s) 421, 1174
StyD4I CCNGG 3 cut(s) 28, 127, 1415
StyI CCWWGG 3 cut(s) 630, 953, 1051
TaaI ACNGT 1 cut(s) 490
TaqII GACCGA 1 cut(s) 605
TasI AATT 4 cut(s) 543, 1093, 1185, 1366
TatI WGTACW 2 cut(s) 369, 1201
TfiI GAWTC 3 cut(s) 934, 1158, 1394
TscAI CASTG 2 cut(s) 493, 874
TseFI GTSAC 1 cut(s) 1231
TseI GCWGC 7 cut(s) 88, 98, 389, 602, 893, 917, 1313
Tsp45I GTSAC 1 cut(s) 1231
TspDTI ATGAA 6 cut(s) 17, 178, 449, 471, 741, 1323
TspRI CASTG 2 cut(s) 493, 874
Van91I CCANNNNNTGG 1 cut(s) 896
VpaK11BI GGWCC 2 cut(s) 310, 617
XapI RAATTY 1 cut(s) 1185
XceI RCATGY 2 cut(s) 118, 1153
XcmI CCANNNNNNNNNTGG 1 cut(s) 578
XmiI GTMKAC 1 cut(s) 959
XspI CTAG 2 cut(s) 806, 1361
ZrmI AGTACT 1 cut(s) 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.