Rmu_sc0004500.1_g000011

Ammonium transporter 3 member 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004500.1
OG0016764
Physical Location & Seq
Reverse (-)
50211 .. 54775
4565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004500.1_g000011.1.cds

Sequence Viewer

Length: 1584 bp
atgaacaacacctatgctctccctcctggcctcatgtatgatgaggccagtccagattggatgagcaaagccgacaacgcatggcagctgaccgctgcaactctggttggcatgcaaagtgttcctggcctcataatcctatatggcggtgcggtgaagaagaaatgggctgtgaactcagctttcatggcattctatgcttttgcttgtgttcttctgtgttgggttggttggggatatcacatgtcttttggaagtccactcttctcatcagctccattttgggggagggctgatgtggcattggaccaaaagtatctttttacgcaggcgtttcttgggaagttcccaaatgccacaatggtgtactttcagtttgtatttgcagcaatcactttaattttgattgctggagctcttctgggaaggatgaacttttatgcttggatgttgtttgtgccactctggctgacattttcatacaccattggagcatatagtatttggagtccaaacgggtggcttgccgtgagaggaataattgattattctggtggttatgtgatccatctctcttctggagttgctggtttcacagcagcctactgggtcggtcctcgttcaaccaaggacagggaaaggttccctccaaacaacattcttcttatgttgacgggagcagggttgctctggatgggatggacagggttcaatggaggagacccttatgtagtcagtatggatgcttcattggctgtcttgaacactcatgtctgcaccgctaccagcttgctaacttggctagcgcttgatgtcatctttttccgcaagccttctgtcatcggagcagttcaaggcatgatcactggtttagtttgcatcaccccagctgctggagttgtgcaaggatgggcagccataataatggggcttctctctggctcaattccttggtacaccatgatggttgtccacaagaaatccaggcttcttcagaaagttgatgacacaatggctgtcctgcacactcatgctatcgccggatcccttggtggactcctcaccggtttctttgcccatccaaagctcaataatttgttctatggctactacgctcaatatgttggtctattttatggttttgacatgcgaaaagcccatctcgggcttcgtcaagtaggaattcaacttatcggggtaatcttcgttgttacactaaatgttgtggtgacaagcctcgtatgccttttggtgcaactcattgtgcctctgagaatgtctgaagaggatttggagattggtgatgaagcagctcatggtgaagaggcttatgctatctggggtcaaggtgaaaagatggacgtagcatatgatttagagtcatctgcaaaacttaaacccaggcccaaaagtgactgcgctacgcctaaacaacacagcccacctcttataatgcaggcccaggaccgggaccaggcccaggcccaattggaaatgctctgggcacccaagcaattgggcactcaagagccagcccatctggaattgctcaggcaacccaagcatttggctcccgagctttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

527

Amino Acids

57.97

Weight (kDa)

7.18

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016764)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29590
malus_domestica MD08G1145400.v1.1 MD15G1121000.v1.1
prunus_persica Prupe.1G473600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298571
rosa_laevigata RLG00000011503
rosa_multiflora Rmu_sc0004500.1_g000011
rosa_roxburghii Rroxscaffold_7G00169530
rosa_rugosa Rorug06G0281000
rosa_samantha Rh6AG392600 Rh6BG400400 Rh6CG406300 Rh6DG393000
rosa_wichuraiana Rw6G034320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1451
AccB1I GGYRCC 1 cut(s) 1504
AccB7I CCANNNNNTGG 1 cut(s) 893
AciI CCGC 5 cut(s) 93, 147, 152, 780, 826
AclWI GGATC 3 cut(s) 559, 1038, 1051
AcsI RAATTY 1 cut(s) 1182
AcuI CTGAAG 2 cut(s) 977, 1302
AfaI GTAC 2 cut(s) 368, 956
AfeI AGCGCT 1 cut(s) 807
AfiI CCNNNNNNNGG 8 cut(s) 284, 633, 893, 1164, 1467, 1468, 1474, 1480
AflIII ACRYGT 1 cut(s) 243
AgeI ACCGGT 1 cut(s) 1064
AgsI TTSAA 5 cut(s) 624, 712, 763, 854, 1187
AjnI CCWGG 7 cut(s) 25, 124, 983, 1400, 1461, 1473, 1479
AleI CACNNNNGTG 1 cut(s) 362
AluBI AGCT 9 cut(s) 88, 182, 275, 416, 789, 890, 1087, 1313, 1579
AluI AGCT 9 cut(s) 88, 182, 275, 416, 789, 890, 1087, 1313, 1579
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 1 cut(s) 714
AlwI GGATC 3 cut(s) 559, 1038, 1051
AlwNI CAGNNNCTG 1 cut(s) 893
Ama87I CYCGRG 2 cut(s) 1163, 1574
Aor51HI AGCGCT 1 cut(s) 807
AoxI GGCC 7 cut(s) 28, 45, 127, 1403, 1458, 1476, 1482
ApeKI GCWGC 7 cut(s) 85, 95, 386, 599, 890, 914, 1310
ApoI RAATTY 1 cut(s) 1182
AsiGI ACCGGT 1 cut(s) 1064
AspLEI GCGC 2 cut(s) 808, 1421
AspS9I GGNCC 8 cut(s) 307, 614, 1404, 1459, 1465, 1471, 1477, 1483
AsuC2I CCSGG 1 cut(s) 1469
AsuHPI GGTGA 7 cut(s) 166, 874, 1054, 1240, 1313, 1331, 1361
AsuNHI GCTAGC 1 cut(s) 802
AvaI CYCGRG 2 cut(s) 1163, 1574
AvaII GGWCC 4 cut(s) 307, 614, 1465, 1471
BaeGI GKGCMC 2 cut(s) 1507, 1523
BamHI GGATCC 1 cut(s) 1043
BanI GGYRCC 1 cut(s) 1504
BanII GRGCYC 1 cut(s) 418
BarI GAAGNNNNNNTAC 2 cut(s) 730, 762
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 7 cut(s) 82, 97, 398, 611, 877, 926, 1322
BccI CCATC 9 cut(s) 576, 688, 693, 903, 958, 1086, 1167, 1351, 1545
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 7 cut(s) 27, 126, 985, 1402, 1463, 1475, 1481
BclI TGATCA 1 cut(s) 861
BcnI CCSGG 1 cut(s) 1469
BcoDI GTCTC 1 cut(s) 714
BfaI CTAG 1 cut(s) 803
BfoI RGCGCY 1 cut(s) 809
BglI GCCNNNNNGGC 1 cut(s) 466
BisI GCNGC 7 cut(s) 86, 96, 387, 600, 891, 915, 1311
BlsI GCNGC 7 cut(s) 87, 97, 388, 601, 892, 916, 1312
Bme1390I CCNGG 8 cut(s) 27, 126, 985, 1402, 1463, 1469, 1475, 1481
Bme18I GGWCC 4 cut(s) 307, 614, 1465, 1471
BmeT110I CYCGRG 2 cut(s) 1163, 1574
BmgT120I GGNCC 8 cut(s) 307, 614, 1404, 1459, 1465, 1471, 1477, 1483
BmiI GGNNCC 5 cut(s) 644, 1045, 1472, 1506, 1572
BmrFI CCNGG 8 cut(s) 27, 126, 985, 1402, 1463, 1469, 1475, 1481
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 2 cut(s) 733, 888
BmtI GCTAGC 1 cut(s) 806
BmuI ACTGGG 1 cut(s) 616
BpmI CTGGAG 3 cut(s) 432, 600, 915
Bpu10I CCTNAGC 1 cut(s) 1550
BpuEI CTTGAG 1 cut(s) 1509
BpuMI CCSGG 1 cut(s) 1469
BsaI GGTCTC 1 cut(s) 714
BsaJI CCNNGG 6 cut(s) 627, 950, 1048, 1400, 1461, 1479
BsaWI WCCGGW 1 cut(s) 1064
Bsc4I CCNNNNNNNGG 8 cut(s) 284, 633, 893, 1164, 1467, 1468, 1474, 1480
Bse118I RCCGGY 1 cut(s) 1064
Bse1I ACTGG 3 cut(s) 48, 611, 871
BseBI CCWGG 7 cut(s) 27, 126, 985, 1402, 1463, 1475, 1481
BseDI CCNNGG 6 cut(s) 627, 950, 1048, 1400, 1461, 1479
BseGI GGATG 8 cut(s) 66, 435, 453, 699, 704, 748, 914, 1078
BseLI CCNNNNNNNGG 8 cut(s) 284, 633, 893, 1164, 1467, 1468, 1474, 1480
BseMII CTCAG 3 cut(s) 192, 1262, 1564
BseNI ACTGG 3 cut(s) 48, 611, 871
BseRI GAGGAG 2 cut(s) 732, 1049
BseSI GKGCMC 2 cut(s) 1507, 1523
BseXI GCAGC 7 cut(s) 82, 97, 398, 611, 877, 926, 1322
BseYI CCCAGC 1 cut(s) 886
BsgI GTGCAG 2 cut(s) 760, 1007
BshFI GGCC 7 cut(s) 30, 47, 129, 1405, 1460, 1478, 1484
BshNI GGYRCC 1 cut(s) 1504
BshTI ACCGGT 1 cut(s) 1064
BsiHKAI GWGCWC 1 cut(s) 418
BsiHKCI CYCGRG 2 cut(s) 1163, 1574
BsiSI CCGG 3 cut(s) 1041, 1065, 1468
BslFI GGGAC 1 cut(s) 1484
BslI CCNNNNNNNGG 8 cut(s) 284, 633, 893, 1164, 1467, 1468, 1474, 1480
BsmAI GTCTC 1 cut(s) 714
BsmFI GGGAC 1 cut(s) 1484
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 7 cut(s) 30, 47, 129, 1405, 1460, 1478, 1484
Bso31I GGTCTC 1 cut(s) 714
BsoBI CYCGRG 2 cut(s) 1163, 1574
Bsp1286I GDGCHC 3 cut(s) 418, 1507, 1523
Bsp143I GATC 3 cut(s) 564, 861, 1043
BspACI CCGC 5 cut(s) 93, 147, 152, 780, 826
BspANI GGCC 7 cut(s) 30, 47, 129, 1405, 1460, 1478, 1484
BspCNI CTCAG 3 cut(s) 191, 1263, 1563
BspLI GGNNCC 5 cut(s) 644, 1045, 1472, 1506, 1572
BspOI GCTAGC 1 cut(s) 806
BspPI GGATC 3 cut(s) 559, 1038, 1051
BspQI GCTCTTC 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 1504
BspTNI GGTCTC 1 cut(s) 714
BsrFI RCCGGY 1 cut(s) 1064
BsrI ACTGG 3 cut(s) 48, 611, 871
BssAI RCCGGY 1 cut(s) 1064
BssECI CCNNGG 6 cut(s) 627, 950, 1048, 1400, 1461, 1479
BssMI GATC 3 cut(s) 564, 861, 1043
BssT1I CCWWGG 3 cut(s) 627, 950, 1048
Bst2UI CCWGG 7 cut(s) 27, 126, 985, 1402, 1463, 1475, 1481
Bst6I CTCTTC 5 cut(s) 269, 423, 580, 1278, 1317
BstAPI GCANNNNNTGC 1 cut(s) 197
BstC8I GCNNGC 8 cut(s) 113, 330, 525, 791, 804, 830, 1458, 1533
BstDEI CTNAG 3 cut(s) 178, 1271, 1550
BstF5I GGATG 8 cut(s) 66, 435, 453, 699, 704, 748, 914, 1078
BstH2I RGCGCY 1 cut(s) 809
BstHHI GCGC 2 cut(s) 808, 1421
BstKTI GATC 3 cut(s) 567, 864, 1046
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 3 cut(s) 564, 861, 1043
BstMWI GCNNNNNNNGC 9 cut(s) 77, 188, 197, 299, 466, 752, 799, 1242, 1561
BstNI CCWGG 7 cut(s) 27, 126, 985, 1402, 1463, 1475, 1481
BstNSI RCATGY 3 cut(s) 115, 247, 1150
BstSCI CCNGG 8 cut(s) 25, 124, 983, 1400, 1461, 1467, 1473, 1479
BstSLI GKGCMC 2 cut(s) 1507, 1523
BstV1I GCAGC 7 cut(s) 82, 97, 398, 611, 877, 926, 1322
BstX2I RGATCY 1 cut(s) 1043
BstXI CCANNNNNNTGG 4 cut(s) 519, 925, 1516, 1567
BstYI RGATCY 1 cut(s) 1043
BsuRI GGCC 7 cut(s) 30, 47, 129, 1405, 1460, 1478, 1484
BtsCI GGATG 8 cut(s) 66, 435, 453, 699, 704, 748, 914, 1078
BtsIMutI CAGTG 1 cut(s) 864
Cac8I GCNNGC 8 cut(s) 113, 330, 525, 791, 804, 830, 1458, 1533
CaiI CAGNNNCTG 1 cut(s) 893
CfoI GCGC 2 cut(s) 808, 1421
Cfr10I RCCGGY 1 cut(s) 1064
Cfr13I GGNCC 8 cut(s) 307, 614, 1404, 1459, 1465, 1471, 1477, 1483
Csp6I GTAC 2 cut(s) 367, 955
CspAI ACCGGT 1 cut(s) 1064
CviQI GTAC 2 cut(s) 367, 955
DdeI CTNAG 3 cut(s) 178, 1271, 1550
DpnI GATC 3 cut(s) 566, 863, 1045
DpnII GATC 3 cut(s) 564, 861, 1043
Eam1104I CTCTTC 5 cut(s) 269, 423, 580, 1278, 1317
EarI CTCTTC 5 cut(s) 269, 423, 580, 1278, 1317
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 3 cut(s) 627, 950, 1048
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 714
Eco32I GATATC 1 cut(s) 239
Eco47I GGWCC 4 cut(s) 307, 614, 1465, 1471
Eco47III AGCGCT 1 cut(s) 807
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 2 cut(s) 977, 1302
Eco88I CYCGRG 2 cut(s) 1163, 1574
EcoICRI GAGCTC 1 cut(s) 416
EcoRI GAATTC 1 cut(s) 1182
EcoRII CCWGG 7 cut(s) 25, 124, 983, 1400, 1461, 1473, 1479
EcoRV GATATC 1 cut(s) 239
EcoT14I CCWWGG 3 cut(s) 627, 950, 1048
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 3 cut(s) 627, 950, 1048
FaqI GGGAC 1 cut(s) 1484
FauNDI CATATG 1 cut(s) 1369
FbaI TGATCA 1 cut(s) 861
Fnu4HI GCNGC 7 cut(s) 86, 96, 387, 600, 891, 915, 1311
FokI GGATG 8 cut(s) 73, 442, 460, 706, 711, 755, 921, 1065
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 7 cut(s) 86, 96, 387, 600, 891, 915, 1311
FspBI CTAG 1 cut(s) 803
GlaI GCGC 2 cut(s) 807, 1420
GluI GCNGC 7 cut(s) 86, 96, 387, 600, 891, 915, 1311
GsaI CCCAGC 1 cut(s) 890
GsuI CTGGAG 3 cut(s) 432, 600, 915
HaeII RGCGCY 1 cut(s) 809
HaeIII GGCC 7 cut(s) 30, 47, 129, 1405, 1460, 1478, 1484
HapII CCGG 3 cut(s) 1041, 1065, 1468
HhaI GCGC 2 cut(s) 808, 1421
Hin6I GCGC 2 cut(s) 806, 1419
HinP1I GCGC 2 cut(s) 806, 1419
HincII GTYRAC 1 cut(s) 672
HindII GTYRAC 1 cut(s) 672
HinfI GANTC 3 cut(s) 508, 1056, 1379
HpaII CCGG 3 cut(s) 1041, 1065, 1468
HphI GGTGA 7 cut(s) 166, 874, 1054, 1240, 1313, 1331, 1361
Hpy166II GTNNAC 7 cut(s) 175, 260, 367, 672, 957, 973, 1055
Hpy188I TCNGA 4 cut(s) 845, 996, 1272, 1282
Hpy188III TCNNGA 7 cut(s) 53, 579, 691, 760, 1526, 1541, 1574
Hpy8I GTNNAC 7 cut(s) 175, 260, 367, 672, 957, 973, 1055
HpyAV CCTTC 2 cut(s) 420, 843
HpyCH4IV ACGT 1 cut(s) 1362
HpyF10VI GCNNNNNNNGC 9 cut(s) 77, 188, 197, 299, 466, 752, 799, 1242, 1561
HpyF3I CTNAG 3 cut(s) 178, 1271, 1550
HpySE526I ACGT 1 cut(s) 1362
HspAI GCGC 2 cut(s) 806, 1419
Ksp22I TGATCA 1 cut(s) 861
Kzo9I GATC 3 cut(s) 564, 861, 1043
LguI GCTCTTC 1 cut(s) 423
LmnI GCTCC 6 cut(s) 280, 413, 491, 677, 845, 1576
Lsp1109I GCAGC 7 cut(s) 82, 97, 398, 611, 877, 926, 1322
LweI GCATC 2 cut(s) 733, 888
MaeI CTAG 1 cut(s) 803
MaeII ACGT 1 cut(s) 1362
MaeIII GTNAC 3 cut(s) 1210, 1228, 1412
MalI GATC 3 cut(s) 566, 863, 1045
MboI GATC 3 cut(s) 564, 861, 1043
MfeI CAATTG 2 cut(s) 1487, 1514
MflI RGATCY 1 cut(s) 1043
MhlI GDGCHC 3 cut(s) 418, 1507, 1523
MluCI AATT 8 cut(s) 399, 540, 945, 1093, 1182, 1487, 1514, 1544
MlyI GAGTC 3 cut(s) 517, 1050, 1388
MseI TTAA 3 cut(s) 398, 1395, 1582
MslI CAYNNNNRTG 4 cut(s) 362, 923, 962, 1029
MspA1I CMGCKG 3 cut(s) 88, 95, 890
MspI CCGG 3 cut(s) 1041, 1065, 1468
MspR9I CCNGG 8 cut(s) 27, 126, 985, 1402, 1463, 1469, 1475, 1481
MunI CAATTG 2 cut(s) 1487, 1514
Mva1269I GAATGC 1 cut(s) 191
MvaI CCWGG 7 cut(s) 27, 126, 985, 1402, 1463, 1475, 1481
MwoI GCNNNNNNNGC 9 cut(s) 77, 188, 197, 299, 466, 752, 799, 1242, 1561
NciI CCSGG 1 cut(s) 1469
NdeI CATATG 1 cut(s) 1369
NdeII GATC 3 cut(s) 564, 861, 1043
NheI GCTAGC 1 cut(s) 802
NlaIV GGNNCC 5 cut(s) 644, 1045, 1472, 1506, 1572
NmuCI GTSAC 2 cut(s) 1228, 1412
NspI RCATGY 3 cut(s) 115, 247, 1150
OliI CACNNNNGTG 1 cut(s) 362
PaeI GCATGC 1 cut(s) 115
PciI ACATGT 1 cut(s) 243
PciSI GCTCTTC 1 cut(s) 423
PctI GAATGC 1 cut(s) 191
PflMI CCANNNNNTGG 1 cut(s) 893
PinAI ACCGGT 1 cut(s) 1064
PkrI GCNGC 7 cut(s) 87, 97, 388, 601, 892, 916, 1312
PleI GAGTC 3 cut(s) 516, 1050, 1387
PpsI GAGTC 3 cut(s) 516, 1050, 1387
PscI ACATGT 1 cut(s) 243
PsiI TTATAA 1 cut(s) 1451
Psp124BI GAGCTC 1 cut(s) 418
Psp6I CCWGG 7 cut(s) 25, 124, 983, 1400, 1461, 1473, 1479
PspFI CCCAGC 1 cut(s) 886
PspGI CCWGG 7 cut(s) 25, 124, 983, 1400, 1461, 1473, 1479
PspN4I GGNNCC 5 cut(s) 644, 1045, 1472, 1506, 1572
PspPI GGNCC 8 cut(s) 307, 614, 1404, 1459, 1465, 1471, 1477, 1483
PstNI CAGNNNCTG 1 cut(s) 893
PsuI RGATCY 1 cut(s) 1043
PvuII CAGCTG 2 cut(s) 88, 890
RsaI GTAC 2 cut(s) 368, 956
RsaNI GTAC 2 cut(s) 367, 955
RseI CAYNNNNRTG 4 cut(s) 362, 923, 962, 1029
SacI GAGCTC 1 cut(s) 418
SapI GCTCTTC 1 cut(s) 423
SaqAI TTAA 3 cut(s) 398, 1395, 1582
SatI GCNGC 7 cut(s) 86, 96, 387, 600, 891, 915, 1311
Sau3AI GATC 3 cut(s) 564, 861, 1043
Sau96I GGNCC 8 cut(s) 307, 614, 1404, 1459, 1465, 1471, 1477, 1483
SchI GAGTC 3 cut(s) 517, 1050, 1388
ScrFI CCNGG 8 cut(s) 27, 126, 985, 1402, 1463, 1469, 1475, 1481
SduI GDGCHC 3 cut(s) 418, 1507, 1523
SfaNI GCATC 2 cut(s) 733, 888
SinI GGWCC 4 cut(s) 307, 614, 1465, 1471
SmiMI CAYNNNNRTG 4 cut(s) 362, 923, 962, 1029
SmlI CTYRAG 1 cut(s) 1524
SmoI CTYRAG 1 cut(s) 1524
SphI GCATGC 1 cut(s) 115
Sse9I AATT 8 cut(s) 399, 540, 945, 1093, 1182, 1487, 1514, 1544
SsiI CCGC 5 cut(s) 93, 147, 152, 780, 826
SspMI CTAG 1 cut(s) 803
SstI GAGCTC 1 cut(s) 418
StyD4I CCNGG 8 cut(s) 25, 124, 983, 1400, 1461, 1467, 1473, 1479
StyI CCWWGG 3 cut(s) 627, 950, 1048
TaiI ACGT 1 cut(s) 1365
TaqII GACCGA 1 cut(s) 602
TasI AATT 8 cut(s) 399, 540, 945, 1093, 1182, 1487, 1514, 1544
TatI WGTACW 1 cut(s) 366
Tru1I TTAA 3 cut(s) 398, 1395, 1582
Tru9I TTAA 3 cut(s) 398, 1395, 1582
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 2 cut(s) 1228, 1412
TseI GCWGC 7 cut(s) 85, 95, 386, 599, 890, 914, 1310
Tsp45I GTSAC 2 cut(s) 1228, 1412
TspDTI ATGAA 6 cut(s) 17, 175, 446, 468, 738, 1320
TspRI CASTG 1 cut(s) 871
Van91I CCANNNNNTGG 1 cut(s) 893
VpaK11BI GGWCC 4 cut(s) 307, 614, 1465, 1471
XapI RAATTY 1 cut(s) 1182
XceI RCATGY 3 cut(s) 115, 247, 1150
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XspI CTAG 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.