Rorug06G0281000

Ammonium transporter 3 member 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001123
Physical Location & Seq
Reverse (-)
42846079 .. 42855323
9245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0281000.1

Sequence Viewer

Length: 1536 bp
ATGCTGATGGCAGAATGTGTGGTATCACCACTATGTATGAAGCTGCAAATGCAGAGGAGTTTCGCCGCAGGGAAGCCCCGGAGTTCACATTCGAAGATGGTGCAAAGGAAGCATTCCTATGGAAGTTGCAAAACTGTTCTGGTTCCAATGTGGAAAATGTGTGACTTCACTGCACCTGGCTTCTCAAATTTGAAAGTTAATCCTTTTCCCGGGGAGGTTGCTTTAAAATGTGCTTGCTTGGGGACTTTGATAGGCTCTGATGGTGCAACGGCCTCTGATTGGATTCCTCTTGTTGATCAACTACTCCTGACGGCCAGTGTGTTTCTCACATATATGGCTGGAGTAATTCCTGGTTACAACTCTAGTCCACCTTCTCAGAATGACAACGCGGTTCCTAAGACCTCAAACGCTTCTGGTAGTGCTACAAAGAACAATGATCCAGATTCAGGTTATGCCGTGGATGCAGTGAAAAAGAAACTTCTAGATTCTCTTGAAGCTCTTGAACTAGGAGGCAATCTGGGAAATAGGGTCCTTGAACCTGAAGGGTATTATGCAAAGAGACCCTTAAGTTTGAATGCTATTGCTAATGGTCCGCGACTACGATTGCTATGGGCATCATTGCAGCGAATTGAGGAAGAGGTCAAAACTATCTCCAATTCTCAAACTTTTAACTCGGATTATTGCTTGAAGAATTTTTCTGGTATTATACAAAAATCCTGTCTCCCTGTATGCACGAGCTGGCTAGAAACAGAACTTGGACTTTTAAACAGCAGACCTAACAAGGCACTTGTTTTGCTGATGGATGCAAAGTTAAATGGAGACAACGCTATTTTACAGAACATCAGGAATTCTGGCAAGGAAGATCTTTATGCAGAATTGCTGTGCTTTCTTAGTTTTGGTTCTCTCAGGGAGGGTTGCTCTTATGACAGTGGCCTGTTTATGATGCATGCAGTTTCCATACTGGAAGATTTGGTGATAACTTTGTCAGATGGGATAGTAAGCATATATTTGGAGCTTATTTCTGTTGACAGCAATTTCTCAGATGAAATGGATAGCTTGTCTTTGGCATTGTGTACTTTGTCAACACGAGCACTACAAAGATTACGTAATGAGGTGGCTTTGTACCAATGGTTGTATCAAAATTTGGAAGCAGTTGCATCAATGTATGAGGATCGATTTGACTTATGGACATTTGAGAGTCAACTCATAGAGAAAGTAGACAATAGCTGGAATCAAAACTACAGTTGGTGGAAGAGACTTATCCATAAAAAATCTGACACTGCATCATCAACATTAGACCATGTTTTGATAAGTAACTTTTCTATGCCTGTAAAGCGAACGAAGGAATTGAGAGCCCTTACAGGCTGGAGGTACTACTTCAGTCTATTCCTCGAGTTATCTGACATTAGCATGCCATTGGTCAGAGCAGTCATTGATAAAGTCAGCAAGGCTATGTCATTTTTTCTAGTCTGCTTGATTGGGAGGTCATTAGGACTCATTTATACTGGAATTAGGCAGTCTCTGCGGTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

511

Amino Acids

57.0

Weight (kDa)

7.86

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3685 PF12452 313 - 406 8.7e-07 Protein of unknown function (DUF3685)
DUF3685 PF12452 432 - 506 5.8e-12 Protein of unknown function (DUF3685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1218
AccII CGCG 2 cut(s) 389, 595
AciI CCGC 4 cut(s) 66, 389, 593, 1525
AclWI GGATC 2 cut(s) 431, 1179
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 4 cut(s) 187, 691, 847, 1141
AcuI CTGAAG 2 cut(s) 561, 1363
AfaI GTAC 3 cut(s) 1075, 1124, 1373
AfiI CCNNNNNNNGG 3 cut(s) 209, 279, 446
AflII CTTAAG 1 cut(s) 565
AgsI TTSAA 6 cut(s) 193, 494, 503, 536, 574, 688
AjnI CCWGG 2 cut(s) 175, 349
AjuI GAANNNNNNNTTGG 2 cut(s) 738, 770
AluBI AGCT 6 cut(s) 43, 497, 738, 1015, 1056, 1227
AluI AGCT 6 cut(s) 43, 497, 738, 1015, 1056, 1227
Alw21I GWGCWC 1 cut(s) 1093
Alw26I GTCTC 5 cut(s) 553, 725, 813, 1249, 1524
AlwI GGATC 2 cut(s) 431, 1179
AlwNI CAGNNNCTG 1 cut(s) 1522
Ama87I CYCGRG 2 cut(s) 209, 1391
AoxI GGCC 3 cut(s) 270, 312, 931
ApeKI GCWGC 2 cut(s) 43, 622
ApoI RAATTY 4 cut(s) 187, 691, 847, 1141
ArsI GACNNNNNNTTYG 2 cut(s) 703, 735
AspS9I GGNCC 2 cut(s) 529, 590
AsuC2I CCSGG 3 cut(s) 79, 210, 211
AsuHPI GGTGA 2 cut(s) 18, 985
AsuII TTCGAA 1 cut(s) 92
AvaI CYCGRG 2 cut(s) 209, 1391
AvaII GGWCC 2 cut(s) 529, 590
BanII GRGCYC 1 cut(s) 1357
BauI CACGAG 2 cut(s) 733, 1086
Bbv12I GWGCWC 1 cut(s) 1093
BbvI GCAGC 2 cut(s) 30, 634
BccI CCATC 4 cut(s) 91, 254, 793, 983
BceAI ACGGC 3 cut(s) 285, 327, 440
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BciT130I CCWGG 2 cut(s) 177, 351
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 3 cut(s) 79, 210, 211
BcoDI GTCTC 5 cut(s) 553, 725, 813, 1249, 1524
BfaI CTAG 5 cut(s) 363, 482, 506, 743, 1466
BfmI CTRYAG 1 cut(s) 1240
BfrI CTTAAG 1 cut(s) 565
BglII AGATCT 1 cut(s) 862
BisI GCNGC 3 cut(s) 44, 66, 623
BlsI GCNGC 3 cut(s) 45, 67, 624
Bme1390I CCNGG 5 cut(s) 79, 177, 210, 211, 351
Bme18I GGWCC 2 cut(s) 529, 590
BmeT110I CYCGRG 2 cut(s) 209, 1391
BmgT120I GGNCC 2 cut(s) 529, 590
BmiI GGNNCC 3 cut(s) 144, 393, 530
BmrFI CCNGG 5 cut(s) 79, 177, 210, 211, 351
BmsI GCATC 6 cut(s) 451, 623, 793, 933, 1166, 1292
BplI GAGNNNNNCTC 2 cut(s) 902, 934
BpmI CTGGAG 2 cut(s) 360, 1387
Bpu14I TTCGAA 1 cut(s) 92
BpuMI CCSGG 3 cut(s) 79, 210, 211
Bsa29I ATCGAT 1 cut(s) 1174
BsaAI YACGTR 1 cut(s) 1106
BsaI GGTCTC 1 cut(s) 553
BsaJI CCNNGG 4 cut(s) 77, 209, 210, 456
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 279, 446
Bse1I ACTGG 3 cut(s) 315, 966, 1510
Bse3DI GCAATG 1 cut(s) 617
BseBI CCWGG 2 cut(s) 177, 351
BseCI ATCGAT 1 cut(s) 1174
BseDI CCNNGG 4 cut(s) 77, 209, 210, 456
BseGI GGATG 2 cut(s) 466, 808
BseLI CCNNNNNNNGG 3 cut(s) 209, 279, 446
BseMI GCAATG 1 cut(s) 617
BseMII CTCAG 3 cut(s) 389, 919, 1053
BseNI ACTGG 3 cut(s) 315, 966, 1510
BseRI GAGGAG 1 cut(s) 70
BseXI GCAGC 2 cut(s) 30, 634
BsgI GTGCAG 1 cut(s) 156
Bsh1236I CGCG 2 cut(s) 389, 595
BshFI GGCC 3 cut(s) 272, 314, 933
BshVI ATCGAT 1 cut(s) 1174
BsiHKAI GWGCWC 1 cut(s) 1093
BsiHKCI CYCGRG 2 cut(s) 209, 1391
BsiSI CCGG 2 cut(s) 79, 210
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 3 cut(s) 209, 279, 446
BsmAI GTCTC 5 cut(s) 553, 725, 813, 1249, 1524
BsmFI GGGAC 1 cut(s) 256
BsmI GAATGC 2 cut(s) 112, 580
BsnI GGCC 3 cut(s) 272, 314, 933
Bso31I GGTCTC 1 cut(s) 553
BsoBI CYCGRG 2 cut(s) 209, 1391
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 2 cut(s) 1093, 1357
Bsp143I GATC 4 cut(s) 295, 436, 862, 1171
BspACI CCGC 4 cut(s) 66, 389, 593, 1525
BspANI GGCC 3 cut(s) 272, 314, 933
BspCNI CTCAG 3 cut(s) 388, 918, 1052
BspDI ATCGAT 1 cut(s) 1174
BspFNI CGCG 2 cut(s) 389, 595
BspLI GGNNCC 3 cut(s) 144, 393, 530
BspPI GGATC 2 cut(s) 431, 1179
BspT104I TTCGAA 1 cut(s) 92
BspTI CTTAAG 1 cut(s) 565
BspTNI GGTCTC 1 cut(s) 553
BsrDI GCAATG 1 cut(s) 617
BsrI ACTGG 3 cut(s) 315, 966, 1510
BssECI CCNNGG 4 cut(s) 77, 209, 210, 456
BssMI GATC 4 cut(s) 295, 436, 862, 1171
BssSI CACGAG 2 cut(s) 733, 1086
Bst2BI CACGAG 2 cut(s) 733, 1086
Bst2UI CCWGG 2 cut(s) 177, 351
Bst4CI ACNGT 3 cut(s) 136, 929, 1244
Bst6I CTCTTC 2 cut(s) 630, 1247
BstAFI CTTAAG 1 cut(s) 565
BstAPI GCANNNNNTGC 1 cut(s) 1522
BstBAI YACGTR 1 cut(s) 1106
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 4 cut(s) 235, 740, 948, 1412
BstDEI CTNAG 5 cut(s) 375, 396, 890, 905, 1039
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 2 cut(s) 466, 808
BstFNI CGCG 2 cut(s) 389, 595
BstKTI GATC 4 cut(s) 298, 439, 865, 1174
BstMAI GTCTC 5 cut(s) 553, 725, 813, 1249, 1524
BstMBI GATC 4 cut(s) 295, 436, 862, 1171
BstMWI GCNNNNNNNGC 5 cut(s) 49, 109, 461, 1333, 1522
BstNI CCWGG 2 cut(s) 177, 351
BstNSI RCATGY 2 cut(s) 950, 1414
BstSCI CCNGG 5 cut(s) 77, 175, 208, 209, 349
BstSFI CTRYAG 1 cut(s) 1240
BstSNI TACGTA 1 cut(s) 1106
BstUI CGCG 2 cut(s) 389, 595
BstV1I GCAGC 2 cut(s) 30, 634
BstX2I RGATCY 1 cut(s) 862
BstYI RGATCY 1 cut(s) 862
Bsu15I ATCGAT 1 cut(s) 1174
BsuRI GGCC 3 cut(s) 272, 314, 933
BsuTUI ATCGAT 1 cut(s) 1174
BtgI CCRYGG 1 cut(s) 456
BtsCI GGATG 2 cut(s) 466, 808
BtsI GCAGTG 3 cut(s) 168, 471, 1278
BtsIMutI CAGTG 5 cut(s) 168, 322, 471, 934, 1278
Cac8I GCNNGC 4 cut(s) 235, 740, 948, 1412
CaiI CAGNNNCTG 1 cut(s) 1522
Cfr13I GGNCC 2 cut(s) 529, 590
Cfr9I CCCGGG 1 cut(s) 209
ClaI ATCGAT 1 cut(s) 1174
Csp6I GTAC 3 cut(s) 1074, 1123, 1372
CviAII CATG 3 cut(s) 947, 1301, 1411
CviQI GTAC 3 cut(s) 1074, 1123, 1372
DdeI CTNAG 5 cut(s) 375, 396, 890, 905, 1039
DpnI GATC 4 cut(s) 297, 438, 864, 1173
DpnII GATC 4 cut(s) 295, 436, 862, 1171
DraI TTTAAA 2 cut(s) 225, 765
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 2 cut(s) 630, 1247
EarI CTCTTC 2 cut(s) 630, 1247
Eco105I TACGTA 1 cut(s) 1106
Eco24I GRGCYC 1 cut(s) 1357
Eco31I GGTCTC 1 cut(s) 553
Eco47I GGWCC 2 cut(s) 529, 590
Eco57I CTGAAG 2 cut(s) 561, 1363
Eco88I CYCGRG 2 cut(s) 209, 1391
EcoO109I RGGNCCY 1 cut(s) 529
EcoRI GAATTC 1 cut(s) 847
EcoRII CCWGG 2 cut(s) 175, 349
EcoT22I ATGCAT 1 cut(s) 948
EcoT38I GRGCYC 1 cut(s) 1357
FaeI CATG 3 cut(s) 950, 1304, 1414
FalI AAGNNNNNCTT 2 cut(s) 548, 580
FaqI GGGAC 1 cut(s) 256
FatI CATG 3 cut(s) 946, 1300, 1410
FbaI TGATCA 1 cut(s) 295
FblI GTMKAC 1 cut(s) 1218
Fnu4HI GCNGC 3 cut(s) 44, 66, 623
FokI GGATG 2 cut(s) 473, 815
FriOI GRGCYC 1 cut(s) 1357
Fsp4HI GCNGC 3 cut(s) 44, 66, 623
FspBI CTAG 5 cut(s) 363, 482, 506, 743, 1466
GluI GCNGC 3 cut(s) 44, 66, 623
GsuI CTGGAG 2 cut(s) 360, 1387
HaeIII GGCC 3 cut(s) 272, 314, 933
HapII CCGG 2 cut(s) 79, 210
Hin1II CATG 3 cut(s) 950, 1304, 1414
HincII GTYRAC 3 cut(s) 1027, 1083, 1202
HindII GTYRAC 3 cut(s) 1027, 1083, 1202
HinfI GANTC 6 cut(s) 283, 443, 485, 1198, 1231, 1494
HpaII CCGG 2 cut(s) 79, 210
HphI GGTGA 2 cut(s) 18, 985
Hpy166II GTNNAC 7 cut(s) 86, 368, 1027, 1074, 1083, 1202, 1219
Hpy188I TCNGA 9 cut(s) 259, 277, 378, 676, 988, 1042, 1276, 1402, 1424
Hpy188III TCNNGA 6 cut(s) 307, 440, 482, 491, 500, 844
Hpy8I GTNNAC 7 cut(s) 86, 368, 1027, 1074, 1083, 1202, 1219
HpyAV CCTTC 3 cut(s) 381, 536, 1336
HpyCH4III ACNGT 3 cut(s) 136, 929, 1244
HpyCH4IV ACGT 1 cut(s) 1105
HpyF10VI GCNNNNNNNGC 5 cut(s) 49, 109, 461, 1333, 1522
HpyF3I CTNAG 5 cut(s) 375, 396, 890, 905, 1039
HpySE526I ACGT 1 cut(s) 1105
Hsp92II CATG 3 cut(s) 950, 1304, 1414
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 4 cut(s) 295, 436, 862, 1171
LmnI GCTCC 1 cut(s) 1012
Lsp1109I GCAGC 2 cut(s) 30, 634
LweI GCATC 6 cut(s) 451, 623, 793, 933, 1166, 1292
MaeI CTAG 5 cut(s) 363, 482, 506, 743, 1466
MaeII ACGT 1 cut(s) 1105
MaeIII GTNAC 3 cut(s) 161, 353, 1313
MalI GATC 4 cut(s) 297, 438, 864, 1173
MboI GATC 4 cut(s) 295, 436, 862, 1171
MboII GAAGA 6 cut(s) 106, 647, 700, 872, 977, 1264
MflI RGATCY 1 cut(s) 862
MhlI GDGCHC 2 cut(s) 1093, 1357
MlyI GAGTC 2 cut(s) 1207, 1488
Mph1103I ATGCAT 1 cut(s) 948
MseI TTAA 6 cut(s) 198, 224, 566, 669, 764, 812
MslI CAYNNNNRTG 4 cut(s) 31, 117, 332, 1409
MspCI CTTAAG 1 cut(s) 565
MspI CCGG 2 cut(s) 79, 210
MspR9I CCNGG 5 cut(s) 79, 177, 210, 211, 351
Mva1269I GAATGC 2 cut(s) 112, 580
MvaI CCWGG 2 cut(s) 177, 351
MvnI CGCG 2 cut(s) 389, 595
MwoI GCNNNNNNNGC 5 cut(s) 49, 109, 461, 1333, 1522
NciI CCSGG 3 cut(s) 79, 210, 211
NdeII GATC 4 cut(s) 295, 436, 862, 1171
NlaIII CATG 3 cut(s) 950, 1304, 1414
NlaIV GGNNCC 3 cut(s) 144, 393, 530
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 948
NspI RCATGY 2 cut(s) 950, 1414
NspV TTCGAA 1 cut(s) 92
PaeI GCATGC 2 cut(s) 950, 1414
PaeR7I CTCGAG 1 cut(s) 1391
PctI GAATGC 2 cut(s) 112, 580
PfeI GAWTC 4 cut(s) 283, 443, 485, 1231
PkrI GCNGC 3 cut(s) 45, 67, 624
PleI GAGTC 2 cut(s) 1206, 1488
PpsI GAGTC 2 cut(s) 1206, 1488
Ppu21I YACGTR 1 cut(s) 1106
PpuMI RGGWCCY 1 cut(s) 529
Psp5II RGGWCCY 1 cut(s) 529
Psp6I CCWGG 2 cut(s) 175, 349
PspGI CCWGG 2 cut(s) 175, 349
PspN4I GGNNCC 3 cut(s) 144, 393, 530
PspPI GGNCC 2 cut(s) 529, 590
PspPPI RGGWCCY 1 cut(s) 529
PspXI VCTCGAGB 1 cut(s) 1391
PstNI CAGNNNCTG 1 cut(s) 1522
PsuI RGATCY 1 cut(s) 862
RsaI GTAC 3 cut(s) 1075, 1124, 1373
RsaNI GTAC 3 cut(s) 1074, 1123, 1372
RseI CAYNNNNRTG 4 cut(s) 31, 117, 332, 1409
SaqAI TTAA 6 cut(s) 198, 224, 566, 669, 764, 812
SatI GCNGC 3 cut(s) 44, 66, 623
Sau3AI GATC 4 cut(s) 295, 436, 862, 1171
Sau96I GGNCC 2 cut(s) 529, 590
SchI GAGTC 2 cut(s) 1207, 1488
ScrFI CCNGG 5 cut(s) 79, 177, 210, 211, 351
SduI GDGCHC 2 cut(s) 1093, 1357
SfaNI GCATC 6 cut(s) 451, 623, 793, 933, 1166, 1292
SfcI CTRYAG 1 cut(s) 1240
Sfr274I CTCGAG 1 cut(s) 1391
SfuI TTCGAA 1 cut(s) 92
SinI GGWCC 2 cut(s) 529, 590
SlaI CTCGAG 1 cut(s) 1391
SmaI CCCGGG 1 cut(s) 211
SmiMI CAYNNNNRTG 4 cut(s) 31, 117, 332, 1409
SmlI CTYRAG 2 cut(s) 565, 1391
SmoI CTYRAG 2 cut(s) 565, 1391
SnaBI TACGTA 1 cut(s) 1106
SphI GCATGC 2 cut(s) 950, 1414
SsiI CCGC 4 cut(s) 66, 389, 593, 1525
SspMI CTAG 5 cut(s) 363, 482, 506, 743, 1466
StyD4I CCNGG 5 cut(s) 77, 175, 208, 209, 349
TaaI ACNGT 3 cut(s) 136, 929, 1244
TaiI ACGT 1 cut(s) 1108
TaqI TCGA 3 cut(s) 92, 1174, 1392
TatI WGTACW 1 cut(s) 1073
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 4 cut(s) 283, 443, 485, 1231
Tru1I TTAA 6 cut(s) 198, 224, 566, 669, 764, 812
Tru9I TTAA 6 cut(s) 198, 224, 566, 669, 764, 812
TscAI CASTG 5 cut(s) 175, 322, 471, 934, 1285
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 2 cut(s) 43, 622
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 2 cut(s) 53, 1059
TspMI CCCGGG 1 cut(s) 209
TspRI CASTG 5 cut(s) 175, 322, 471, 934, 1285
Vha464I CTTAAG 1 cut(s) 565
VpaK11BI GGWCC 2 cut(s) 529, 590
XapI RAATTY 4 cut(s) 187, 691, 847, 1141
XbaI TCTAGA 1 cut(s) 481
XceI RCATGY 2 cut(s) 950, 1414
XhoI CTCGAG 1 cut(s) 1391
XmaI CCCGGG 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 1218
XspI CTAG 5 cut(s) 363, 482, 506, 743, 1466
Zsp2I ATGCAT 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.