Rh6BG400400

Ammonium transporter 3 member 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0016764
Physical Location & Seq
Reverse (-)
62781073 .. 62783512
2440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG400400.1

Sequence Viewer

Length: 1431 bp
ATGAACAACACCTATGCTCTCCCTCCTGGCCTCATGTATGATGAGGCCAGTCCAGACTGGATGAGCAAAGCCGACAACGCATGGCAGCTGACCGCTGCAACTCTGGTTGGCATGCAAAGTGTTCCTGGCCTCATAATCCTATATGGCGGTGCGGTGAAGAAGAAATGGGCTGTGAACTCAGCTTTCATGGCATTCTATGCTTTTGCTTGTGTTCTTCTGTGTTGGGTTGGTTGGGGATATCACATGTCTTTTGGAAGTCCACTCTTCTCATCAGCTCCATTTTGGGGGAAGGCTGATGTGGCATTGGACCAAAAGTATCTTTTTACGCAGGCGTTTCTTGGGAAGTTCCCAAATGCCACAATGGTGTACTTTCAGTTTGTATTTGCAGCAATCACTTTAATTTTGATTGCTGGAGCCCTTCTGGGAAGGATGAACTTTTATGCTTGGATGTTGTTTGTGCCACTCTGGCTGACATTTTCATACACCATTGGAGCATATAGTATTTGGAGTCCAAACGGGTGGCTTGCCTTGAGAGGAATAATTGATTATTCTGGTGGTTATGTGATCCATCTCTCTTCTGGAGTTGCTGGTTTCACAGCAGCCTACTGGGTCGGTCCTCGTTCAACCAAGGACAGGGAAAGGTTCCCTCCAAACAACATTCTTCTTATGTTGACGGGAGCAGGGTTGCTCTGGATGGGATGGACAGGGTTCAATGGAGGAGACCCTTATGTAGTCAGTATGGATGCTTCATTGGCTGTCTTGAACACTCATGTCTGCACCGCTACCAGCTTGCTAACTTGGCTAGCGCTTGATGTCATCTTTTTCCGCAAGCCTTCTGTCATCGGAGCAGTTCAAGGCATGATCACTGGTTTAGTTTGCATCACCCCAGCTGCTGGAGTTGTGCAAGGATGGGCAGCCATAATAATGGGGCTTCTCTCCGGCTCAATTCCTTGGTACACCATGATGGTTGTCCACAAGAAATCTAGGCTTCTTCAGAGAGTTGATGACACAATGGCTGTCCTACACACTCATGCTATCGCCGGATCCCTTGGTGGACTCCTCACCGGTTTCTTTGCCCATCCAAAGCTCAATAATTTGTTCTATGGCTACTATGCTCAATATGTTGGTCTATTTTATGGTTTTGACATGGGAAAAGTCCATCTCGGGCTTCGTCAAGTAGGAATTCAACTTCTCGGGGTAATCTTCGTTGTTACACTAAATGTTGTTGTGACAAGCCTCGTATGCCTTTTGGTGCAACTCATTGTGCCTCTGAGAATGTCTGAAGAGGATATGGAGATTGGTGATGAAGCAGCTCATGGTGAAGAGGCTTATGCTATCTGGGGTCAAGGTGAAAAGATGGACGTAGCATATGATTTAGAGTCATCTGCAAAAAGTAAACCCAGGTCTGCTGGAAATATTGAGATGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

476

Amino Acids

52.04

Weight (kDa)

6.75

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 27 - 444 8.5e-81 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016764)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29590
malus_domestica MD08G1145400.v1.1 MD15G1121000.v1.1
prunus_persica Prupe.1G473600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298571
rosa_laevigata RLG00000011503
rosa_multiflora Rmu_sc0004500.1_g000011
rosa_roxburghii Rroxscaffold_7G00169530
rosa_rugosa Rorug06G0281000
rosa_samantha Rh6AG392600 Rh6BG400400 Rh6CG406300 Rh6DG393000
rosa_wichuraiana Rw6G034320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 893
AciI CCGC 5 cut(s) 93, 147, 152, 780, 826
AclWI GGATC 3 cut(s) 559, 1038, 1051
AcsI RAATTY 1 cut(s) 1182
AcuI CTGAAG 2 cut(s) 977, 1302
AfaI GTAC 2 cut(s) 368, 956
AfeI AGCGCT 1 cut(s) 807
AfiI CCNNNNNNNGG 3 cut(s) 284, 633, 893
AflIII ACRYGT 1 cut(s) 243
AgeI ACCGGT 1 cut(s) 1064
AgsI TTSAA 5 cut(s) 624, 712, 763, 854, 1187
AjnI CCWGG 3 cut(s) 25, 124, 1400
AleI CACNNNNGTG 1 cut(s) 362
AluBI AGCT 7 cut(s) 88, 182, 275, 789, 890, 1087, 1313
AluI AGCT 7 cut(s) 88, 182, 275, 789, 890, 1087, 1313
Alw26I GTCTC 1 cut(s) 714
AlwI GGATC 3 cut(s) 559, 1038, 1051
AlwNI CAGNNNCTG 1 cut(s) 893
Ama87I CYCGRG 2 cut(s) 1163, 1193
Aor51HI AGCGCT 1 cut(s) 807
AoxI GGCC 3 cut(s) 28, 45, 127
ApeKI GCWGC 7 cut(s) 85, 95, 386, 599, 890, 914, 1310
ApoI RAATTY 1 cut(s) 1182
AsiGI ACCGGT 1 cut(s) 1064
AspLEI GCGC 1 cut(s) 808
AspS9I GGNCC 2 cut(s) 307, 614
AsuHPI GGTGA 6 cut(s) 166, 874, 1054, 1313, 1331, 1361
AsuNHI GCTAGC 1 cut(s) 802
AvaI CYCGRG 2 cut(s) 1163, 1193
AvaII GGWCC 2 cut(s) 307, 614
BamHI GGATCC 1 cut(s) 1043
BanII GRGCYC 1 cut(s) 418
BarI GAAGNNNNNNTAC 2 cut(s) 730, 762
BbvI GCAGC 7 cut(s) 82, 97, 398, 611, 877, 926, 1322
BccI CCATC 9 cut(s) 576, 688, 693, 903, 958, 1086, 1167, 1351, 1417
BciT130I CCWGG 3 cut(s) 27, 126, 1402
BclI TGATCA 1 cut(s) 861
BcoDI GTCTC 1 cut(s) 714
BfaI CTAG 2 cut(s) 803, 984
BfoI RGCGCY 1 cut(s) 809
BglI GCCNNNNNGGC 1 cut(s) 466
BisI GCNGC 7 cut(s) 86, 96, 387, 600, 891, 915, 1311
BlsI GCNGC 7 cut(s) 87, 97, 388, 601, 892, 916, 1312
Bme1390I CCNGG 3 cut(s) 27, 126, 1402
Bme18I GGWCC 2 cut(s) 307, 614
BmeT110I CYCGRG 2 cut(s) 1163, 1193
BmgT120I GGNCC 2 cut(s) 307, 614
BmiI GGNNCC 3 cut(s) 415, 644, 1045
BmrFI CCNGG 3 cut(s) 27, 126, 1402
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 2 cut(s) 733, 888
BmtI GCTAGC 1 cut(s) 806
BmuI ACTGGG 1 cut(s) 616
BpmI CTGGAG 3 cut(s) 432, 600, 915
BpuEI CTTGAG 1 cut(s) 550
BsaI GGTCTC 1 cut(s) 714
BsaJI CCNNGG 4 cut(s) 627, 950, 1048, 1400
BsaWI WCCGGW 1 cut(s) 1064
Bsc4I CCNNNNNNNGG 3 cut(s) 284, 633, 893
Bse118I RCCGGY 1 cut(s) 1064
Bse1I ACTGG 4 cut(s) 48, 62, 611, 871
BseBI CCWGG 3 cut(s) 27, 126, 1402
BseDI CCNNGG 4 cut(s) 627, 950, 1048, 1400
BseGI GGATG 8 cut(s) 66, 435, 453, 699, 704, 748, 914, 1078
BseLI CCNNNNNNNGG 3 cut(s) 284, 633, 893
BseMII CTCAG 2 cut(s) 192, 1262
BseNI ACTGG 4 cut(s) 48, 62, 611, 871
BseRI GAGGAG 2 cut(s) 732, 1049
BseXI GCAGC 7 cut(s) 82, 97, 398, 611, 877, 926, 1322
BseYI CCCAGC 1 cut(s) 886
BsgI GTGCAG 1 cut(s) 760
BshFI GGCC 3 cut(s) 30, 47, 129
BshTI ACCGGT 1 cut(s) 1064
BsiHKCI CYCGRG 2 cut(s) 1163, 1193
BsiSI CCGG 3 cut(s) 939, 1041, 1065
BslI CCNNNNNNNGG 3 cut(s) 284, 633, 893
BsmAI GTCTC 1 cut(s) 714
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 3 cut(s) 30, 47, 129
Bso31I GGTCTC 1 cut(s) 714
BsoBI CYCGRG 2 cut(s) 1163, 1193
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 3 cut(s) 564, 861, 1043
BspACI CCGC 5 cut(s) 93, 147, 152, 780, 826
BspANI GGCC 3 cut(s) 30, 47, 129
BspCNI CTCAG 2 cut(s) 191, 1263
BspLI GGNNCC 3 cut(s) 415, 644, 1045
BspOI GCTAGC 1 cut(s) 806
BspPI GGATC 3 cut(s) 559, 1038, 1051
BspTNI GGTCTC 1 cut(s) 714
BsrFI RCCGGY 1 cut(s) 1064
BsrI ACTGG 4 cut(s) 48, 62, 611, 871
BssAI RCCGGY 1 cut(s) 1064
BssECI CCNNGG 4 cut(s) 627, 950, 1048, 1400
BssMI GATC 3 cut(s) 564, 861, 1043
BssT1I CCWWGG 3 cut(s) 627, 950, 1048
Bst2UI CCWGG 3 cut(s) 27, 126, 1402
Bst6I CTCTTC 4 cut(s) 269, 580, 1278, 1317
BstAPI GCANNNNNTGC 1 cut(s) 197
BstC8I GCNNGC 6 cut(s) 113, 330, 525, 791, 804, 830
BstDEI CTNAG 2 cut(s) 178, 1271
BstF5I GGATG 8 cut(s) 66, 435, 453, 699, 704, 748, 914, 1078
BstH2I RGCGCY 1 cut(s) 809
BstHHI GCGC 1 cut(s) 808
BstKTI GATC 3 cut(s) 567, 864, 1046
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 3 cut(s) 564, 861, 1043
BstMWI GCNNNNNNNGC 8 cut(s) 77, 188, 197, 299, 466, 752, 799, 1242
BstNI CCWGG 3 cut(s) 27, 126, 1402
BstNSI RCATGY 2 cut(s) 115, 247
BstSCI CCNGG 3 cut(s) 25, 124, 1400
BstV1I GCAGC 7 cut(s) 82, 97, 398, 611, 877, 926, 1322
BstX2I RGATCY 1 cut(s) 1043
BstXI CCANNNNNNTGG 2 cut(s) 519, 925
BstYI RGATCY 1 cut(s) 1043
BsuRI GGCC 3 cut(s) 30, 47, 129
BtsCI GGATG 8 cut(s) 66, 435, 453, 699, 704, 748, 914, 1078
BtsIMutI CAGTG 1 cut(s) 864
Cac8I GCNNGC 6 cut(s) 113, 330, 525, 791, 804, 830
CaiI CAGNNNCTG 1 cut(s) 893
CfoI GCGC 1 cut(s) 808
Cfr10I RCCGGY 1 cut(s) 1064
Cfr13I GGNCC 2 cut(s) 307, 614
Csp6I GTAC 2 cut(s) 367, 955
CspAI ACCGGT 1 cut(s) 1064
CviQI GTAC 2 cut(s) 367, 955
DdeI CTNAG 2 cut(s) 178, 1271
DpnI GATC 3 cut(s) 566, 863, 1045
DpnII GATC 3 cut(s) 564, 861, 1043
Eam1104I CTCTTC 4 cut(s) 269, 580, 1278, 1317
EarI CTCTTC 4 cut(s) 269, 580, 1278, 1317
Eco130I CCWWGG 3 cut(s) 627, 950, 1048
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 714
Eco32I GATATC 1 cut(s) 239
Eco47I GGWCC 2 cut(s) 307, 614
Eco47III AGCGCT 1 cut(s) 807
Eco57I CTGAAG 2 cut(s) 977, 1302
Eco88I CYCGRG 2 cut(s) 1163, 1193
EcoRI GAATTC 1 cut(s) 1182
EcoRII CCWGG 3 cut(s) 25, 124, 1400
EcoRV GATATC 1 cut(s) 239
EcoT14I CCWWGG 3 cut(s) 627, 950, 1048
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 3 cut(s) 627, 950, 1048
FauNDI CATATG 1 cut(s) 1369
FbaI TGATCA 1 cut(s) 861
Fnu4HI GCNGC 7 cut(s) 86, 96, 387, 600, 891, 915, 1311
FokI GGATG 8 cut(s) 73, 442, 460, 706, 711, 755, 921, 1065
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 7 cut(s) 86, 96, 387, 600, 891, 915, 1311
FspBI CTAG 2 cut(s) 803, 984
GlaI GCGC 1 cut(s) 807
GluI GCNGC 7 cut(s) 86, 96, 387, 600, 891, 915, 1311
GsaI CCCAGC 1 cut(s) 890
GsuI CTGGAG 3 cut(s) 432, 600, 915
HaeII RGCGCY 1 cut(s) 809
HaeIII GGCC 3 cut(s) 30, 47, 129
HapII CCGG 3 cut(s) 939, 1041, 1065
HhaI GCGC 1 cut(s) 808
Hin6I GCGC 1 cut(s) 806
HinP1I GCGC 1 cut(s) 806
HincII GTYRAC 1 cut(s) 672
HindII GTYRAC 1 cut(s) 672
HinfI GANTC 3 cut(s) 508, 1056, 1379
HpaII CCGG 3 cut(s) 939, 1041, 1065
HphI GGTGA 6 cut(s) 166, 874, 1054, 1313, 1331, 1361
Hpy166II GTNNAC 8 cut(s) 175, 260, 367, 672, 957, 973, 1055, 1397
Hpy188I TCNGA 4 cut(s) 845, 996, 1272, 1282
Hpy188III TCNNGA 4 cut(s) 53, 579, 691, 760
Hpy8I GTNNAC 8 cut(s) 175, 260, 367, 672, 957, 973, 1055, 1397
HpyAV CCTTC 4 cut(s) 283, 420, 428, 843
HpyCH4IV ACGT 1 cut(s) 1362
HpyCH4V TGCA 8 cut(s) 98, 115, 386, 777, 879, 904, 1255, 1388
HpyF10VI GCNNNNNNNGC 8 cut(s) 77, 188, 197, 299, 466, 752, 799, 1242
HpyF3I CTNAG 2 cut(s) 178, 1271
HpySE526I ACGT 1 cut(s) 1362
HspAI GCGC 1 cut(s) 806
Ksp22I TGATCA 1 cut(s) 861
Kzo9I GATC 3 cut(s) 564, 861, 1043
LmnI GCTCC 5 cut(s) 280, 413, 491, 677, 845
Lsp1109I GCAGC 7 cut(s) 82, 97, 398, 611, 877, 926, 1322
LweI GCATC 2 cut(s) 733, 888
MaeI CTAG 2 cut(s) 803, 984
MaeII ACGT 1 cut(s) 1362
MaeIII GTNAC 2 cut(s) 1210, 1228
MalI GATC 3 cut(s) 566, 863, 1045
MboI GATC 3 cut(s) 564, 861, 1043
MflI RGATCY 1 cut(s) 1043
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 5 cut(s) 399, 540, 945, 1093, 1182
MlyI GAGTC 3 cut(s) 517, 1050, 1388
MseI TTAA 1 cut(s) 398
MslI CAYNNNNRTG 4 cut(s) 362, 923, 962, 1029
MspA1I CMGCKG 3 cut(s) 88, 95, 890
MspI CCGG 3 cut(s) 939, 1041, 1065
MspR9I CCNGG 3 cut(s) 27, 126, 1402
Mva1269I GAATGC 1 cut(s) 191
MvaI CCWGG 3 cut(s) 27, 126, 1402
MwoI GCNNNNNNNGC 8 cut(s) 77, 188, 197, 299, 466, 752, 799, 1242
NdeI CATATG 1 cut(s) 1369
NdeII GATC 3 cut(s) 564, 861, 1043
NheI GCTAGC 1 cut(s) 802
NlaIV GGNNCC 3 cut(s) 415, 644, 1045
NmuCI GTSAC 1 cut(s) 1228
NspI RCATGY 2 cut(s) 115, 247
OliI CACNNNNGTG 1 cut(s) 362
PaeI GCATGC 1 cut(s) 115
PciI ACATGT 1 cut(s) 243
PctI GAATGC 1 cut(s) 191
PflMI CCANNNNNTGG 1 cut(s) 893
PinAI ACCGGT 1 cut(s) 1064
PkrI GCNGC 7 cut(s) 87, 97, 388, 601, 892, 916, 1312
PleI GAGTC 3 cut(s) 516, 1050, 1387
PpsI GAGTC 3 cut(s) 516, 1050, 1387
PscI ACATGT 1 cut(s) 243
Psp6I CCWGG 3 cut(s) 25, 124, 1400
PspFI CCCAGC 1 cut(s) 886
PspGI CCWGG 3 cut(s) 25, 124, 1400
PspN4I GGNNCC 3 cut(s) 415, 644, 1045
PspPI GGNCC 2 cut(s) 307, 614
PstNI CAGNNNCTG 1 cut(s) 893
PsuI RGATCY 1 cut(s) 1043
PvuII CAGCTG 2 cut(s) 88, 890
RsaI GTAC 2 cut(s) 368, 956
RsaNI GTAC 2 cut(s) 367, 955
RseI CAYNNNNRTG 4 cut(s) 362, 923, 962, 1029
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 7 cut(s) 86, 96, 387, 600, 891, 915, 1311
Sau3AI GATC 3 cut(s) 564, 861, 1043
Sau96I GGNCC 2 cut(s) 307, 614
SchI GAGTC 3 cut(s) 517, 1050, 1388
ScrFI CCNGG 3 cut(s) 27, 126, 1402
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 2 cut(s) 733, 888
SinI GGWCC 2 cut(s) 307, 614
SmiMI CAYNNNNRTG 4 cut(s) 362, 923, 962, 1029
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
SphI GCATGC 1 cut(s) 115
Sse9I AATT 5 cut(s) 399, 540, 945, 1093, 1182
SsiI CCGC 5 cut(s) 93, 147, 152, 780, 826
SspI AATATT 1 cut(s) 1417
SspMI CTAG 2 cut(s) 803, 984
StyD4I CCNGG 3 cut(s) 25, 124, 1400
StyI CCWWGG 3 cut(s) 627, 950, 1048
TaiI ACGT 1 cut(s) 1365
TaqII GACCGA 1 cut(s) 602
TasI AATT 5 cut(s) 399, 540, 945, 1093, 1182
TatI WGTACW 1 cut(s) 366
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 1 cut(s) 1228
TseI GCWGC 7 cut(s) 85, 95, 386, 599, 890, 914, 1310
Tsp45I GTSAC 1 cut(s) 1228
TspDTI ATGAA 6 cut(s) 17, 175, 446, 468, 738, 1320
TspRI CASTG 1 cut(s) 871
Van91I CCANNNNNTGG 1 cut(s) 893
VpaK11BI GGWCC 2 cut(s) 307, 614
XapI RAATTY 1 cut(s) 1182
XceI RCATGY 2 cut(s) 115, 247
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XspI CTAG 2 cut(s) 803, 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.