MD08G1145400.v1.1

Ammonium transporter 3 member 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016764
Physical Location & Seq
Forward (+)
14460558 .. 14462974
2417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1145400.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGAGCAACAGCACAGTTGCTTTGCCTATTAACCTCATACCAGATGAGGCCAGTCCGGCATGGATGAACAAAGGCGACAACGCGTGGCAGCTAACAGCCGCGACTTTGGTCGGCCTCCAGAGTATCCCGGGGCTCATGATCCTATACGGTGGCGCAGTGAAGAAGAAATGGGCTGTGAACTCGGCTTTCATGGGATTCTATGCTTTTGCTTGTGTTCTTGTTTGTTGGGTTGGGTGGGGATACCATATGTCGTTTGGGGATGCCTTAATCAAGAATATTCCATTTTGGGGGAAACCTAACGTGGCACTGAACCAGCAATATCTTTTGGGCATAGCATTTAGTGGGAAAATACCAAATGCCACAATGGTTTATTTCCAATTTGTGTTTGCAGCAATTACTTTGATTCTAATTGCTGGGGCACTTTTGGGAAGGATGAACTTTTATGCTTGGATGTTGTTTGTGCCTTTGTGGCTAACTTTTTCATACACCATTGCAGCTTTTAGTATTTGGAGCCCTAACGGGTTTCTGGCCAAGAAAGGAATCATTGATTATTCTGGTGGGTACGTGATCCATCTTTCTTCTGGTGCTGCAGGGTTCACAGCTGCCTACTGGGTTGGTCCTCGTTCGAACAAAGACAGGGAAAGGTTTCCTCCCAACAACATTCTTCTTATGTTGACTGGTGCAGGACTTCTCTGGATGGGCTGGACAGGGTTTAATGGAGGAGACCCTTATGTGGTCAGTGTTGATGCCTCCTTGGCTGTGCTGAACACTCACATCTGCACTGCTACCAGTTTACTCACTTGGCTCTTGCTTGACATCACTTTCTTCCGTAAACCTTCGGTCATTGGGGCAGTTCAGGGAATGATCACTGGCTTAGTTTGCATCACCCCTGCTGCTGGTGTTGTGCAAGGATGGGCAGCAATAATAATGGGGATATTTTCGGGCTCAATCCCTTGGTTTACCATGATGGTTGTTCACAAGAGATCTTTGCTCCTTCAGAAAGTTGACGACACAATGGCTGTCCTCCACACTCATGCCATCGCAGGCGCCCTTGGTGGAATCCTCACCGGTCTCTTTGCCCACCCACGGCTTAGCTTCTTGTTCTATACCAAATATGGACAGTACGTTGGCTTATTCTACGGTTTCCATATGGGAGAAGTCAGCACCGGCTTTCGTCAAGTGGGAATCCAACTTCTCGGGATCTTTTATGTTGTTACACTAAATGTTGTGGTCACAAGCATTATATGTCTTGTGATACAATCCATCATGCCGCTGCGAATGTCCACGGAGGATATGGAGATTGGTGATGAAGCAGCTCATGGGGAAGAAGCTTATGCTATCTGGGGACAAGGTGAAAAGCTTGGAAATTCAAAGTCTGGAGGATATGATATTGAATCATCAGCTGCCAAAAGCAGAAAAGCTGCTCGACAAATTGAAATGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

482

Amino Acids

52.27

Weight (kDa)

8.79

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 28 - 445 8.8e-80 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016764)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29590
malus_domestica MD08G1145400.v1.1 MD15G1121000.v1.1
prunus_persica Prupe.1G473600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298571
rosa_laevigata RLG00000011503
rosa_multiflora Rmu_sc0004500.1_g000011
rosa_roxburghii Rroxscaffold_7G00169530
rosa_rugosa Rorug06G0281000
rosa_samantha Rh6AG392600 Rh6BG400400 Rh6CG406300 Rh6DG393000
rosa_wichuraiana Rw6G034320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1046
AccII CGCG 2 cut(s) 83, 101
AciI CCGC 2 cut(s) 99, 1271
AclWI GGATC 3 cut(s) 133, 562, 1208
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 1 cut(s) 1366
AcuI CTGAAG 1 cut(s) 980
AcyI GRCGYC 1 cut(s) 1047
AfaI GTAC 2 cut(s) 563, 1124
AfiI CCNNNNNNNGG 4 cut(s) 287, 733, 896, 1086
AflIII ACRYGT 1 cut(s) 81
AgeI ACCGGT 1 cut(s) 1067
AgsI TTSAA 3 cut(s) 1371, 1394, 1436
AluBI AGCT 9 cut(s) 91, 497, 602, 1095, 1316, 1331, 1360, 1403, 1421
AluI AGCT 9 cut(s) 91, 497, 602, 1095, 1316, 1331, 1360, 1403, 1421
Alw26I GTCTC 2 cut(s) 717, 1076
AlwI GGATC 3 cut(s) 133, 562, 1208
Ama87I CYCGRG 2 cut(s) 127, 1196
AoxI GGCC 3 cut(s) 48, 112, 528
ApoI RAATTY 1 cut(s) 1366
AsiGI ACCGGT 1 cut(s) 1067
Asp700I GAANNNNTTC 1 cut(s) 645
AspLEI GCGC 2 cut(s) 155, 1049
AspS9I GGNCC 1 cut(s) 617
AsuC2I CCSGG 2 cut(s) 128, 129
AsuHPI GGTGA 4 cut(s) 877, 1057, 1316, 1364
AsuII TTCGAA 1 cut(s) 626
AvaI CYCGRG 2 cut(s) 127, 1196
AvaII GGWCC 1 cut(s) 617
BaeGI GKGCMC 1 cut(s) 421
BalI TGGCCA 1 cut(s) 530
BanI GGYRCC 1 cut(s) 1046
BanII GRGCYC 3 cut(s) 135, 515, 947
BccI CCATC 6 cut(s) 579, 691, 906, 961, 1046, 1271
BceAI ACGGC 1 cut(s) 1103
BciVI GTATCC 2 cut(s) 134, 233
BclI TGATCA 1 cut(s) 864
BcnI CCSGG 2 cut(s) 128, 129
BcoDI GTCTC 2 cut(s) 717, 1076
BfmI CTRYAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 1050
BfuI GTATCC 2 cut(s) 134, 233
BglI GCCNNNNNGGC 3 cut(s) 56, 469, 755
BglII AGATCT 1 cut(s) 983
BlpI GCTNAGC 1 cut(s) 1091
Bme1390I CCNGG 2 cut(s) 128, 129
Bme18I GGWCC 1 cut(s) 617
BmeT110I CYCGRG 2 cut(s) 127, 1196
BmgT120I GGNCC 1 cut(s) 617
BmiI GGNNCC 2 cut(s) 512, 1048
BmrFI CCNGG 2 cut(s) 128, 129
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 3 cut(s) 250, 736, 891
BmuI ACTGGG 1 cut(s) 619
BoxI GACNNNNGTC 1 cut(s) 107
BpmI CTGGAG 2 cut(s) 101, 1398
Bpu1102I GCTNAGC 1 cut(s) 1091
Bpu14I TTCGAA 1 cut(s) 626
BpuMI CCSGG 2 cut(s) 128, 129
BsaAI YACGTR 1 cut(s) 565
BsaHI GRCGYC 1 cut(s) 1047
BsaI GGTCTC 2 cut(s) 717, 1076
BsaJI CCNNGG 7 cut(s) 127, 128, 753, 953, 1051, 1085, 1284
BsaWI WCCGGW 1 cut(s) 1067
Bsc4I CCNNNNNNNGG 4 cut(s) 287, 733, 896, 1086
Bse118I RCCGGY 2 cut(s) 1067, 1166
Bse1I ACTGG 5 cut(s) 51, 614, 682, 789, 874
Bse3DI GCAATG 1 cut(s) 489
BseDI CCNNGG 7 cut(s) 127, 128, 753, 953, 1051, 1085, 1284
BseGI GGATG 6 cut(s) 69, 265, 438, 456, 702, 917
BseLI CCNNNNNNNGG 4 cut(s) 287, 733, 896, 1086
BseMI GCAATG 1 cut(s) 489
BseNI ACTGG 5 cut(s) 51, 614, 682, 789, 874
BseRI GAGGAG 1 cut(s) 735
BseSI GKGCMC 1 cut(s) 421
BseYI CCCAGC 1 cut(s) 413
BsgI GTGCAG 2 cut(s) 702, 763
Bsh1236I CGCG 2 cut(s) 83, 101
BshFI GGCC 3 cut(s) 50, 114, 530
BshNI GGYRCC 1 cut(s) 1046
BshTI ACCGGT 1 cut(s) 1067
BsiHKCI CYCGRG 2 cut(s) 127, 1196
BsiSI CCGG 4 cut(s) 56, 128, 1068, 1167
BslFI GGGAC 1 cut(s) 1359
BslI CCNNNNNNNGG 4 cut(s) 287, 733, 896, 1086
BsmAI GTCTC 2 cut(s) 717, 1076
BsmFI GGGAC 1 cut(s) 1359
BsnI GGCC 3 cut(s) 50, 114, 530
Bso31I GGTCTC 2 cut(s) 717, 1076
BsoBI CYCGRG 2 cut(s) 127, 1196
Bsp119I TTCGAA 1 cut(s) 626
Bsp1286I GDGCHC 4 cut(s) 135, 421, 515, 947
Bsp143I GATC 5 cut(s) 138, 567, 864, 983, 1200
Bsp1720I GCTNAGC 1 cut(s) 1091
BspACI CCGC 2 cut(s) 99, 1271
BspANI GGCC 3 cut(s) 50, 114, 530
BspFNI CGCG 2 cut(s) 83, 101
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 2 cut(s) 512, 1048
BspMAI CTGCAG 1 cut(s) 592
BspPI GGATC 3 cut(s) 133, 562, 1208
BspT104I TTCGAA 1 cut(s) 626
BspT107I GGYRCC 1 cut(s) 1046
BspTNI GGTCTC 2 cut(s) 717, 1076
BsrDI GCAATG 1 cut(s) 489
BsrFI RCCGGY 2 cut(s) 1067, 1166
BsrI ACTGG 5 cut(s) 51, 614, 682, 789, 874
BssAI RCCGGY 2 cut(s) 1067, 1166
BssECI CCNNGG 7 cut(s) 127, 128, 753, 953, 1051, 1085, 1284
BssMI GATC 5 cut(s) 138, 567, 864, 983, 1200
BssNI GRCGYC 1 cut(s) 1047
BssT1I CCWWGG 3 cut(s) 753, 953, 1051
Bst4CI ACNGT 4 cut(s) 16, 149, 1122, 1142
BstACI GRCGYC 1 cut(s) 1047
BstBAI YACGTR 1 cut(s) 565
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 1 cut(s) 1045
BstDEI CTNAG 2 cut(s) 874, 1091
BstDSI CCRYGG 2 cut(s) 1085, 1284
BstF5I GGATG 6 cut(s) 69, 265, 438, 456, 702, 917
BstFNI CGCG 2 cut(s) 83, 101
BstH2I RGCGCY 1 cut(s) 1050
BstHHI GCGC 2 cut(s) 155, 1049
BstKTI GATC 5 cut(s) 141, 570, 867, 986, 1203
BstMAI GTCTC 2 cut(s) 717, 1076
BstMBI GATC 5 cut(s) 138, 567, 864, 983, 1200
BstMWI GCNNNNNNNGC 4 cut(s) 56, 469, 755, 879
BstPAI GACNNNNGTC 1 cut(s) 107
BstSCI CCNGG 2 cut(s) 126, 127
BstSFI CTRYAG 1 cut(s) 588
BstSLI GKGCMC 1 cut(s) 421
BstUI CGCG 2 cut(s) 83, 101
BstX2I RGATCY 2 cut(s) 983, 1200
BstYI RGATCY 2 cut(s) 983, 1200
BsuI GTATCC 2 cut(s) 134, 233
BsuRI GGCC 3 cut(s) 50, 114, 530
BtgI CCRYGG 2 cut(s) 1085, 1284
BtgZI GCGATG 1 cut(s) 1024
BtsCI GGATG 6 cut(s) 69, 265, 438, 456, 702, 917
BtsI GCAGTG 2 cut(s) 162, 780
BtsIMutI CAGTG 5 cut(s) 162, 305, 745, 780, 867
Cac8I GCNNGC 1 cut(s) 1045
CciI TCATGA 1 cut(s) 135
CfoI GCGC 2 cut(s) 155, 1049
Cfr10I RCCGGY 2 cut(s) 1067, 1166
Cfr13I GGNCC 1 cut(s) 617
Cfr9I CCCGGG 1 cut(s) 127
Csp6I GTAC 2 cut(s) 562, 1123
CspAI ACCGGT 1 cut(s) 1067
CviAII CATG 7 cut(s) 60, 136, 190, 964, 1034, 1267, 1319
CviQI GTAC 2 cut(s) 562, 1123
DdeI CTNAG 2 cut(s) 874, 1091
DinI GGCGCC 1 cut(s) 1048
DpnI GATC 5 cut(s) 140, 569, 866, 985, 1202
DpnII GATC 5 cut(s) 138, 567, 864, 983, 1200
EaeI YGGCCR 1 cut(s) 528
Eco130I CCWWGG 3 cut(s) 753, 953, 1051
Eco24I GRGCYC 3 cut(s) 135, 515, 947
Eco31I GGTCTC 2 cut(s) 717, 1076
Eco47I GGWCC 1 cut(s) 617
Eco57I CTGAAG 1 cut(s) 980
Eco88I CYCGRG 2 cut(s) 127, 1196
EcoT14I CCWWGG 3 cut(s) 753, 953, 1051
EcoT38I GRGCYC 3 cut(s) 135, 515, 947
EgeI GGCGCC 1 cut(s) 1048
EheI GGCGCC 1 cut(s) 1048
ErhI CCWWGG 3 cut(s) 753, 953, 1051
FaeI CATG 7 cut(s) 63, 139, 193, 967, 1037, 1270, 1322
FaqI GGGAC 1 cut(s) 1359
FatI CATG 7 cut(s) 59, 135, 189, 963, 1033, 1266, 1318
FauNDI CATATG 2 cut(s) 246, 1149
FbaI TGATCA 1 cut(s) 864
FokI GGATG 6 cut(s) 76, 272, 445, 463, 709, 924
FriOI GRGCYC 3 cut(s) 135, 515, 947
GlaI GCGC 2 cut(s) 154, 1048
GsaI CCCAGC 1 cut(s) 417
GsuI CTGGAG 2 cut(s) 101, 1398
HaeII RGCGCY 1 cut(s) 1050
HaeIII GGCC 3 cut(s) 50, 114, 530
HapII CCGG 4 cut(s) 56, 128, 1068, 1167
HhaI GCGC 2 cut(s) 155, 1049
Hin1I GRCGYC 1 cut(s) 1047
Hin1II CATG 7 cut(s) 63, 139, 193, 967, 1037, 1270, 1322
Hin6I GCGC 2 cut(s) 153, 1047
HinP1I GCGC 2 cut(s) 153, 1047
HincII GTYRAC 2 cut(s) 675, 1006
HindII GTYRAC 2 cut(s) 675, 1006
HindIII AAGCTT 2 cut(s) 1329, 1358
HinfI GANTC 6 cut(s) 195, 403, 540, 1059, 1185, 1394
HpaII CCGG 4 cut(s) 56, 128, 1068, 1167
HphI GGTGA 4 cut(s) 877, 1057, 1316, 1364
Hpy166II GTNNAC 9 cut(s) 178, 597, 675, 794, 833, 960, 976, 1006, 1284
Hpy188I TCNGA 1 cut(s) 999
Hpy188III TCNNGA 6 cut(s) 118, 136, 271, 694, 1198, 1377
Hpy8I GTNNAC 9 cut(s) 178, 597, 675, 794, 833, 960, 976, 1006, 1284
HpyAV CCTTC 3 cut(s) 423, 846, 1004
HpyCH4III ACNGT 4 cut(s) 16, 149, 1122, 1142
HpyCH4IV ACGT 3 cut(s) 300, 564, 1125
HpyCH4V TGCA 7 cut(s) 389, 494, 590, 683, 780, 882, 907
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 469, 755, 879
HpyF3I CTNAG 2 cut(s) 874, 1091
HpySE526I ACGT 3 cut(s) 300, 564, 1125
Hsp92I GRCGYC 1 cut(s) 1047
Hsp92II CATG 7 cut(s) 63, 139, 193, 967, 1037, 1270, 1322
HspAI GCGC 2 cut(s) 153, 1047
KasI GGCGCC 1 cut(s) 1046
Ksp22I TGATCA 1 cut(s) 864
Kzo9I GATC 5 cut(s) 138, 567, 864, 983, 1200
LmnI GCTCC 2 cut(s) 510, 996
LweI GCATC 3 cut(s) 250, 736, 891
MaeII ACGT 3 cut(s) 300, 564, 1125
MaeIII GTNAC 2 cut(s) 1213, 1231
MalI GATC 5 cut(s) 140, 569, 866, 985, 1202
MboI GATC 5 cut(s) 138, 567, 864, 983, 1200
MboII GAAGA 6 cut(s) 172, 175, 570, 656, 817, 1337
MflI RGATCY 2 cut(s) 983, 1200
MhlI GDGCHC 4 cut(s) 135, 421, 515, 947
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 5 cut(s) 377, 393, 408, 1366, 1431
MluI ACGCGT 1 cut(s) 81
MluNI TGGCCA 1 cut(s) 530
Mly113I GGCGCC 1 cut(s) 1047
MmeI TCCRAC 1 cut(s) 1213
Mox20I TGGCCA 1 cut(s) 530
MroXI GAANNNNTTC 1 cut(s) 645
MscI TGGCCA 1 cut(s) 530
MseI TTAA 4 cut(s) 30, 266, 714, 1444
MslI CAYNNNNRTG 1 cut(s) 1032
Msp20I TGGCCA 1 cut(s) 530
MspA1I CMGCKG 3 cut(s) 602, 1273, 1403
MspI CCGG 4 cut(s) 56, 128, 1068, 1167
MspR9I CCNGG 2 cut(s) 128, 129
MvnI CGCG 2 cut(s) 83, 101
MwoI GCNNNNNNNGC 4 cut(s) 56, 469, 755, 879
NarI GGCGCC 1 cut(s) 1047
NciI CCSGG 2 cut(s) 128, 129
NdeI CATATG 2 cut(s) 246, 1149
NdeII GATC 5 cut(s) 138, 567, 864, 983, 1200
NlaIII CATG 7 cut(s) 63, 139, 193, 967, 1037, 1270, 1322
NlaIV GGNNCC 2 cut(s) 512, 1048
NmeAIII GCCGAG 1 cut(s) 161
NmuCI GTSAC 1 cut(s) 1231
NspV TTCGAA 1 cut(s) 626
PagI TCATGA 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 645
PfeI GAWTC 6 cut(s) 195, 403, 540, 1059, 1185, 1394
PinAI ACCGGT 1 cut(s) 1067
PluTI GGCGCC 1 cut(s) 1050
Ppu21I YACGTR 1 cut(s) 565
PshAI GACNNNNGTC 1 cut(s) 107
PspFI CCCAGC 1 cut(s) 413
PspN4I GGNNCC 2 cut(s) 512, 1048
PspPI GGNCC 1 cut(s) 617
PstI CTGCAG 1 cut(s) 592
PsuI RGATCY 2 cut(s) 983, 1200
PvuII CAGCTG 2 cut(s) 602, 1403
RsaI GTAC 2 cut(s) 563, 1124
RsaNI GTAC 2 cut(s) 562, 1123
RseI CAYNNNNRTG 1 cut(s) 1032
SaqAI TTAA 4 cut(s) 30, 266, 714, 1444
Sau3AI GATC 5 cut(s) 138, 567, 864, 983, 1200
Sau96I GGNCC 1 cut(s) 617
ScrFI CCNGG 2 cut(s) 128, 129
SduI GDGCHC 4 cut(s) 135, 421, 515, 947
SfaNI GCATC 3 cut(s) 250, 736, 891
SfcI CTRYAG 1 cut(s) 588
SfoI GGCGCC 1 cut(s) 1048
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 1 cut(s) 617
SmaI CCCGGG 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 1032
Sse9I AATT 5 cut(s) 377, 393, 408, 1366, 1431
SsiI CCGC 2 cut(s) 99, 1271
SspDI GGCGCC 1 cut(s) 1046
SspI AATATT 1 cut(s) 277
StyD4I CCNGG 2 cut(s) 126, 127
StyI CCWWGG 3 cut(s) 753, 953, 1051
TaaI ACNGT 4 cut(s) 16, 149, 1122, 1142
TaiI ACGT 3 cut(s) 303, 567, 1128
TaqI TCGA 2 cut(s) 626, 1426
TaqII GACCGA 1 cut(s) 829
TasI AATT 5 cut(s) 377, 393, 408, 1366, 1431
TauI GCSGC 2 cut(s) 101, 1273
TfiI GAWTC 6 cut(s) 195, 403, 540, 1059, 1185, 1394
Tru1I TTAA 4 cut(s) 30, 266, 714, 1444
Tru9I TTAA 4 cut(s) 30, 266, 714, 1444
TscAI CASTG 5 cut(s) 162, 312, 745, 787, 874
TseFI GTSAC 1 cut(s) 1231
Tsp45I GTSAC 1 cut(s) 1231
TspDTI ATGAA 5 cut(s) 80, 178, 449, 471, 1323
TspGWI ACGGA 2 cut(s) 818, 1301
TspMI CCCGGG 1 cut(s) 127
TspRI CASTG 5 cut(s) 162, 312, 745, 787, 874
VpaK11BI GGWCC 1 cut(s) 617
XapI RAATTY 1 cut(s) 1366
XcmI CCANNNNNNNNNTGG 3 cut(s) 251, 578, 1291
XmaI CCCGGG 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.