MD15G1121000.v1.1

Ammonium transporter 3 member 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016764
Physical Location & Seq
Forward (+)
8692003 .. 8694083
2081 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1121000.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGAGCAACATCACATACGCTCCTTTGCCTATTAACCTCATACCGGATGAGGCCAGTCCGGCATGGATGAACAAAGGTGACAACGCGTGGCAGCTGACAGCCGCAACTTTGGTCGGCCTCCAGAGTGTCCCGGGGCTCATAATCCTATACGGTGGTGCGGTGAAGAAGAAATGGGCTGTGAACTCGGCTTTCATGGCACTCTATGCATTTGCTTGTGTTCTTATTTGTTGGGTTGGTTGGGGATACCAAATGTCATTTGGGGATGTCCTAATCAAGGGTCTTCGGTTTTGGGGGAAACCTGACGTGGCACTTAACCAGGAGTATCTCTTGGAGCAGGCATTTATGGGGAAAATACCAAATGCCACAATGGTTTATTTCCAATTTGTGTTTGCAGCAATTACTTTGATTTTAATTGCTGGGGCGCTTTTGGGAAGGATGAACTTTTATGCTTGGATGTTGTTTGTGCCTTTATGGCTAACTTTTTCATACACCATTGGAGCTTTTAGTATTTGGAGCCCTAATGGTTTTCTTTCCAGGAGAGGACTCATTGATTATTCTGGTGGGTATGTGATCCATCTTTCTTCTGGTGTTGCTGGGTTCACAGCTGCCTATTGGGTTGGTCCACGTTCGAAGAAAGACAGGGCAAGTTTTCCTCCCAACAACATTCTTCTTATGTTGACTGGTGCAGGACTTCTCTGGATGGGCTGGACAGGGTTTAATGGCGGAGACCCTTACGTGGTCAGTGTTGATGCCTCCTTGGCTGTGCTGAACACTCATGTCTGCACTGCTACCAGTTTGCTCACTTGGCTTTTGCTTGACATCACTTTCTTCCGTAAACCTTCGGTCATCGGGGCAGTTCAGGGAATGATCACTGGTTTAGTTTGCATCACCCCTGCTGCTGGTGTTGTGCAAGGCTGGGCAGCAATACTAATGGGGTTATTTTCTGGCTCAATCCCTTGGTTTACCATGATGGTTGTCCACAAGAGATCTTTGCTCCTTCAGAAAGTTGACGACACAATGGCCGTCTTCCACACTCATGCCATTGCCGGCGGCCTTGGTGGAATCCTCACCGGTCTCTTTGGCCACCCGCGGCTTAGCTTCTTGTTCTATAACACTTACGGAAACTATGTTGGCCTATTCTATGGTTTCCAGATGGGAGGTCCCAACGTGCACACCGGCTTTCGTCAAGTGGGAATCCAACTGCTCGGGATCTTTTACATTGTGACACTAAATGTTGTGGTTACAAGCATTATATGTTTTCTGATTCGGTTCATCGTGCCGCTACGGATGTCGGCGGAGGATATGGAGATTGGTGATGAAGCAGCTCATGGGGAAGAAGCTTATGCTATCTGGGGACAAGGCGAAAAGCTTGACAATTCAAGGTTTGGAGGATATGATATTGAATCATCAGCTGCCAAAAGTAGAGCAGCTGCTGGACAAATTGAAATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

485

Amino Acids

52.74

Weight (kDa)

8.17

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 29 - 448 1.1e-78 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016764)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29590
malus_domestica MD08G1145400.v1.1 MD15G1121000.v1.1
prunus_persica Prupe.1G473600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298571
rosa_laevigata RLG00000011503
rosa_multiflora Rmu_sc0004500.1_g000011
rosa_roxburghii Rroxscaffold_7G00169530
rosa_rugosa Rorug06G0281000
rosa_samantha Rh6AG392600 Rh6BG400400 Rh6CG406300 Rh6DG393000
rosa_wichuraiana Rw6G034320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 86, 1090
AciI CCGC 8 cut(s) 102, 158, 723, 1050, 1088, 1090, 1280, 1295
AclWI GGATC 2 cut(s) 565, 1217
AcoI YGGCCR 2 cut(s) 1020, 1081
AcuI CTGAAG 1 cut(s) 983
AfiI CCNNNNNNNGG 4 cut(s) 43, 274, 736, 899
AflIII ACRYGT 1 cut(s) 84
AgeI ACCGGT 1 cut(s) 1070
AgsI TTSAA 3 cut(s) 1380, 1403, 1445
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 2 cut(s) 315, 533
AluBI AGCT 9 cut(s) 94, 500, 605, 1098, 1325, 1340, 1369, 1412, 1430
AluI AGCT 9 cut(s) 94, 500, 605, 1098, 1325, 1340, 1369, 1412, 1430
Alw21I GWGCWC 1 cut(s) 1173
Alw26I GTCTC 2 cut(s) 720, 1079
Alw44I GTGCAC 1 cut(s) 1169
AlwI GGATC 2 cut(s) 565, 1217
AlwNI CAGNNNCTG 1 cut(s) 1433
Ama87I CYCGRG 2 cut(s) 130, 1205
AoxI GGCC 6 cut(s) 51, 115, 1020, 1051, 1081, 1132
ApaLI GTGCAC 1 cut(s) 1169
ApeKI GCWGC 9 cut(s) 91, 392, 605, 896, 920, 1322, 1412, 1427, 1430
AsiGI ACCGGT 1 cut(s) 1070
AspLEI GCGC 1 cut(s) 424
AspS9I GGNCC 2 cut(s) 620, 1160
AsuC2I CCSGG 2 cut(s) 131, 132
AsuHPI GGTGA 5 cut(s) 89, 172, 880, 1060, 1325
AsuII TTCGAA 1 cut(s) 629
AvaI CYCGRG 2 cut(s) 130, 1205
AvaII GGWCC 2 cut(s) 620, 1160
BaeGI GKGCMC 1 cut(s) 1173
BalI TGGCCA 1 cut(s) 1083
BanII GRGCYC 2 cut(s) 138, 518
BbsI GAAGAC 2 cut(s) 272, 1018
Bbv12I GWGCWC 1 cut(s) 1173
BbvI GCAGC 9 cut(s) 103, 404, 592, 883, 932, 1334, 1399, 1417, 1439
BccI CCATC 4 cut(s) 582, 694, 964, 1147
BceAI ACGGC 1 cut(s) 1007
BciT130I CCWGG 2 cut(s) 317, 535
BciVI GTATCC 1 cut(s) 236
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 2 cut(s) 131, 132
BcoDI GTCTC 2 cut(s) 720, 1079
BfoI RGCGCY 1 cut(s) 425
BfuI GTATCC 1 cut(s) 236
BglI GCCNNNNNGGC 3 cut(s) 59, 472, 758
BglII AGATCT 1 cut(s) 986
BlpI GCTNAGC 1 cut(s) 1094
Bme1390I CCNGG 4 cut(s) 131, 132, 317, 535
Bme18I GGWCC 2 cut(s) 620, 1160
BmeT110I CYCGRG 2 cut(s) 130, 1205
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 2 cut(s) 620, 1160
BmiI GGNNCC 2 cut(s) 515, 1162
BmrFI CCNGG 4 cut(s) 131, 132, 317, 535
BmsI GCATC 2 cut(s) 739, 894
BpiI GAAGAC 2 cut(s) 272, 1018
BpmI CTGGAG 1 cut(s) 104
Bpu1102I GCTNAGC 1 cut(s) 1094
Bpu14I TTCGAA 1 cut(s) 629
BpuMI CCSGG 2 cut(s) 131, 132
BsaAI YACGTR 1 cut(s) 736
BsaI GGTCTC 2 cut(s) 720, 1079
BsaJI CCNNGG 6 cut(s) 130, 131, 756, 956, 1054, 1088
BsaWI WCCGGW 2 cut(s) 43, 1070
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 274, 736, 899
Bse118I RCCGGY 3 cut(s) 1046, 1070, 1175
Bse1I ACTGG 4 cut(s) 54, 685, 792, 877
Bse3DI GCAATG 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 317, 535
BseDI CCNNGG 6 cut(s) 130, 131, 756, 956, 1054, 1088
BseGI GGATG 7 cut(s) 52, 72, 268, 441, 459, 705, 1293
BseLI CCNNNNNNNGG 4 cut(s) 43, 274, 736, 899
BseMI GCAATG 1 cut(s) 1041
BseNI ACTGG 4 cut(s) 54, 685, 792, 877
BseSI GKGCMC 1 cut(s) 1173
BseXI GCAGC 9 cut(s) 103, 404, 592, 883, 932, 1334, 1399, 1417, 1439
BseYI CCCAGC 3 cut(s) 416, 593, 915
BsgI GTGCAG 2 cut(s) 705, 766
Bsh1236I CGCG 2 cut(s) 86, 1090
BshFI GGCC 6 cut(s) 53, 117, 1022, 1053, 1083, 1134
BshTI ACCGGT 1 cut(s) 1070
BsiHKAI GWGCWC 1 cut(s) 1173
BsiHKCI CYCGRG 2 cut(s) 130, 1205
BsiSI CCGG 6 cut(s) 44, 59, 131, 1047, 1071, 1176
BslFI GGGAC 3 cut(s) 113, 1146, 1368
BslI CCNNNNNNNGG 4 cut(s) 43, 274, 736, 899
BsmAI GTCTC 2 cut(s) 720, 1079
BsmFI GGGAC 3 cut(s) 113, 1146, 1368
BsnI GGCC 6 cut(s) 53, 117, 1022, 1053, 1083, 1134
Bso31I GGTCTC 2 cut(s) 720, 1079
BsoBI CYCGRG 2 cut(s) 130, 1205
Bsp119I TTCGAA 1 cut(s) 629
Bsp1286I GDGCHC 3 cut(s) 138, 518, 1173
Bsp143I GATC 4 cut(s) 570, 867, 986, 1209
Bsp1720I GCTNAGC 1 cut(s) 1094
BspACI CCGC 8 cut(s) 102, 158, 723, 1050, 1088, 1090, 1280, 1295
BspANI GGCC 6 cut(s) 53, 117, 1022, 1053, 1083, 1134
BspFNI CGCG 2 cut(s) 86, 1090
BspLI GGNNCC 2 cut(s) 515, 1162
BspPI GGATC 2 cut(s) 565, 1217
BspT104I TTCGAA 1 cut(s) 629
BspTNI GGTCTC 2 cut(s) 720, 1079
BsrDI GCAATG 1 cut(s) 1041
BsrFI RCCGGY 3 cut(s) 1046, 1070, 1175
BsrI ACTGG 4 cut(s) 54, 685, 792, 877
BssAI RCCGGY 3 cut(s) 1046, 1070, 1175
BssECI CCNNGG 6 cut(s) 130, 131, 756, 956, 1054, 1088
BssMI GATC 4 cut(s) 570, 867, 986, 1209
BssT1I CCWWGG 3 cut(s) 756, 956, 1054
Bst2UI CCWGG 2 cut(s) 317, 535
Bst4CI ACNGT 1 cut(s) 152
BstAPI GCANNNNNTGC 1 cut(s) 203
BstBAI YACGTR 1 cut(s) 736
BstBI TTCGAA 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 336, 1048
BstDEI CTNAG 1 cut(s) 1094
BstDSI CCRYGG 1 cut(s) 1088
BstENI CCTNNNNNAGG 1 cut(s) 272
BstF5I GGATG 7 cut(s) 52, 72, 268, 441, 459, 705, 1293
BstFNI CGCG 2 cut(s) 86, 1090
BstH2I RGCGCY 1 cut(s) 425
BstHHI GCGC 1 cut(s) 424
BstKTI GATC 4 cut(s) 573, 870, 989, 1212
BstMAI GTCTC 2 cut(s) 720, 1079
BstMBI GATC 4 cut(s) 570, 867, 986, 1209
BstMWI GCNNNNNNNGC 6 cut(s) 59, 194, 203, 472, 758, 805
BstNI CCWGG 2 cut(s) 317, 535
BstSCI CCNGG 4 cut(s) 129, 130, 315, 533
BstSLI GKGCMC 1 cut(s) 1173
BstUI CGCG 2 cut(s) 86, 1090
BstV1I GCAGC 9 cut(s) 103, 404, 592, 883, 932, 1334, 1399, 1417, 1439
BstV2I GAAGAC 2 cut(s) 272, 1018
BstX2I RGATCY 2 cut(s) 986, 1209
BstYI RGATCY 2 cut(s) 986, 1209
BsuI GTATCC 1 cut(s) 236
BsuRI GGCC 6 cut(s) 53, 117, 1022, 1053, 1083, 1134
BtgI CCRYGG 1 cut(s) 1088
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 7 cut(s) 52, 72, 268, 441, 459, 705, 1293
BtsI GCAGTG 1 cut(s) 783
BtsIMutI CAGTG 3 cut(s) 748, 783, 870
Cac8I GCNNGC 2 cut(s) 336, 1048
CaiI CAGNNNCTG 1 cut(s) 1433
CfoI GCGC 1 cut(s) 424
Cfr10I RCCGGY 3 cut(s) 1046, 1070, 1175
Cfr13I GGNCC 2 cut(s) 620, 1160
Cfr42I CCGCGG 1 cut(s) 1091
Cfr9I CCCGGG 1 cut(s) 130
CspAI ACCGGT 1 cut(s) 1070
CviAII CATG 6 cut(s) 63, 193, 776, 967, 1037, 1328
DdeI CTNAG 1 cut(s) 1094
DpnI GATC 4 cut(s) 572, 869, 988, 1211
DpnII GATC 4 cut(s) 570, 867, 986, 1209
EaeI YGGCCR 2 cut(s) 1020, 1081
EciI GGCGGA 2 cut(s) 738, 1310
Eco130I CCWWGG 3 cut(s) 756, 956, 1054
Eco24I GRGCYC 2 cut(s) 138, 518
Eco31I GGTCTC 2 cut(s) 720, 1079
Eco47I GGWCC 2 cut(s) 620, 1160
Eco57I CTGAAG 1 cut(s) 983
Eco88I CYCGRG 2 cut(s) 130, 1205
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 1160
EcoRII CCWGG 2 cut(s) 315, 533
EcoT14I CCWWGG 3 cut(s) 756, 956, 1054
EcoT22I ATGCAT 1 cut(s) 208
EcoT38I GRGCYC 2 cut(s) 138, 518
ErhI CCWWGG 3 cut(s) 756, 956, 1054
FaeI CATG 6 cut(s) 66, 196, 779, 970, 1040, 1331
FaqI GGGAC 3 cut(s) 113, 1146, 1368
FatI CATG 6 cut(s) 62, 192, 775, 966, 1036, 1327
FauI CCCGC 1 cut(s) 1095
FbaI TGATCA 1 cut(s) 867
FokI GGATG 7 cut(s) 59, 79, 275, 448, 466, 712, 1300
FriOI GRGCYC 2 cut(s) 138, 518
GlaI GCGC 1 cut(s) 423
GsaI CCCAGC 3 cut(s) 420, 597, 919
GsuI CTGGAG 1 cut(s) 104
HaeII RGCGCY 1 cut(s) 425
HaeIII GGCC 6 cut(s) 53, 117, 1022, 1053, 1083, 1134
HapII CCGG 6 cut(s) 44, 59, 131, 1047, 1071, 1176
HhaI GCGC 1 cut(s) 424
Hin1II CATG 6 cut(s) 66, 196, 779, 970, 1040, 1331
Hin6I GCGC 1 cut(s) 422
HinP1I GCGC 1 cut(s) 422
HincII GTYRAC 2 cut(s) 678, 1009
HindII GTYRAC 2 cut(s) 678, 1009
HindIII AAGCTT 2 cut(s) 1338, 1367
HinfI GANTC 5 cut(s) 543, 1062, 1194, 1264, 1403
HpaII CCGG 6 cut(s) 44, 59, 131, 1047, 1071, 1176
HphI GGTGA 5 cut(s) 89, 172, 880, 1060, 1325
Hpy166II GTNNAC 9 cut(s) 181, 600, 623, 678, 836, 963, 979, 1009, 1171
Hpy188I TCNGA 2 cut(s) 1002, 1263
Hpy188III TCNNGA 4 cut(s) 121, 697, 1150, 1207
Hpy8I GTNNAC 9 cut(s) 181, 600, 623, 678, 836, 963, 979, 1009, 1171
HpyAV CCTTC 3 cut(s) 426, 849, 1007
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4IV ACGT 4 cut(s) 303, 625, 735, 1167
HpyCH4V TGCA 7 cut(s) 206, 392, 686, 783, 885, 910, 1171
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 194, 203, 472, 758, 805
HpyF3I CTNAG 1 cut(s) 1094
HpySE526I ACGT 4 cut(s) 303, 625, 735, 1167
Hsp92II CATG 6 cut(s) 66, 196, 779, 970, 1040, 1331
HspAI GCGC 1 cut(s) 422
KroI GCCGGC 1 cut(s) 1046
KroNI GCCGGC 1 cut(s) 1048
Ksp22I TGATCA 1 cut(s) 867
KspI CCGCGG 1 cut(s) 1091
Kzo9I GATC 4 cut(s) 570, 867, 986, 1209
LmnI GCTCC 5 cut(s) 25, 331, 497, 513, 999
Lsp1109I GCAGC 9 cut(s) 103, 404, 592, 883, 932, 1334, 1399, 1417, 1439
LweI GCATC 2 cut(s) 739, 894
MaeII ACGT 4 cut(s) 303, 625, 735, 1167
MaeIII GTNAC 3 cut(s) 77, 1222, 1240
MalI GATC 4 cut(s) 572, 869, 988, 1211
MboI GATC 4 cut(s) 570, 867, 986, 1209
MboII GAAGA 9 cut(s) 175, 178, 272, 573, 643, 659, 820, 1018, 1346
MflI RGATCY 2 cut(s) 986, 1209
MhlI GDGCHC 3 cut(s) 138, 518, 1173
MlsI TGGCCA 1 cut(s) 1083
MluCI AATT 5 cut(s) 380, 396, 411, 1375, 1440
MluI ACGCGT 1 cut(s) 84
MluNI TGGCCA 1 cut(s) 1083
MlyI GAGTC 1 cut(s) 537
MmeI TCCRAC 1 cut(s) 1222
Mox20I TGGCCA 1 cut(s) 1083
Mph1103I ATGCAT 1 cut(s) 208
MroNI GCCGGC 1 cut(s) 1046
MscI TGGCCA 1 cut(s) 1083
MseI TTAA 5 cut(s) 33, 312, 410, 717, 1453
MslI CAYNNNNRTG 1 cut(s) 1035
Msp20I TGGCCA 1 cut(s) 1083
MspA1I CMGCKG 5 cut(s) 94, 605, 1090, 1412, 1430
MspI CCGG 6 cut(s) 44, 59, 131, 1047, 1071, 1176
MspR9I CCNGG 4 cut(s) 131, 132, 317, 535
MvaI CCWGG 2 cut(s) 317, 535
MvnI CGCG 2 cut(s) 86, 1090
MwoI GCNNNNNNNGC 6 cut(s) 59, 194, 203, 472, 758, 805
NaeI GCCGGC 1 cut(s) 1048
NciI CCSGG 2 cut(s) 131, 132
NdeII GATC 4 cut(s) 570, 867, 986, 1209
NgoMIV GCCGGC 1 cut(s) 1046
NlaIII CATG 6 cut(s) 66, 196, 779, 970, 1040, 1331
NlaIV GGNNCC 2 cut(s) 515, 1162
NmeAIII GCCGAG 1 cut(s) 164
NmuCI GTSAC 2 cut(s) 77, 1222
NsiI ATGCAT 1 cut(s) 208
NspV TTCGAA 1 cut(s) 629
PdiI GCCGGC 1 cut(s) 1048
PfeI GAWTC 4 cut(s) 1062, 1194, 1264, 1403
PfoI TCCNGGA 1 cut(s) 533
PinAI ACCGGT 1 cut(s) 1070
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
Ppu21I YACGTR 1 cut(s) 736
PpuMI RGGWCCY 1 cut(s) 1160
Psp5II RGGWCCY 1 cut(s) 1160
Psp6I CCWGG 2 cut(s) 315, 533
PspFI CCCAGC 3 cut(s) 416, 593, 915
PspGI CCWGG 2 cut(s) 315, 533
PspN4I GGNNCC 2 cut(s) 515, 1162
PspPI GGNCC 2 cut(s) 620, 1160
PspPPI RGGWCCY 1 cut(s) 1160
PstNI CAGNNNCTG 1 cut(s) 1433
PsuI RGATCY 2 cut(s) 986, 1209
PvuII CAGCTG 4 cut(s) 94, 605, 1412, 1430
RseI CAYNNNNRTG 1 cut(s) 1035
SacII CCGCGG 1 cut(s) 1091
SaqAI TTAA 5 cut(s) 33, 312, 410, 717, 1453
Sau3AI GATC 4 cut(s) 570, 867, 986, 1209
Sau96I GGNCC 2 cut(s) 620, 1160
SchI GAGTC 1 cut(s) 537
ScrFI CCNGG 4 cut(s) 131, 132, 317, 535
SduI GDGCHC 3 cut(s) 138, 518, 1173
SfaNI GCATC 2 cut(s) 739, 894
Sfr303I CCGCGG 1 cut(s) 1091
SfuI TTCGAA 1 cut(s) 629
SgrBI CCGCGG 1 cut(s) 1091
SinI GGWCC 2 cut(s) 620, 1160
SmaI CCCGGG 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 1035
Sse9I AATT 5 cut(s) 380, 396, 411, 1375, 1440
SsiI CCGC 8 cut(s) 102, 158, 723, 1050, 1088, 1090, 1280, 1295
StyD4I CCNGG 4 cut(s) 129, 130, 315, 533
StyI CCWWGG 3 cut(s) 756, 956, 1054
TaaI ACNGT 1 cut(s) 152
TaiI ACGT 4 cut(s) 306, 628, 738, 1170
TaqI TCGA 1 cut(s) 629
TaqII GACCGA 1 cut(s) 832
TasI AATT 5 cut(s) 380, 396, 411, 1375, 1440
TauI GCSGC 4 cut(s) 104, 1053, 1093, 1282
TfiI GAWTC 4 cut(s) 1062, 1194, 1264, 1403
Tru1I TTAA 5 cut(s) 33, 312, 410, 717, 1453
Tru9I TTAA 5 cut(s) 33, 312, 410, 717, 1453
TscAI CASTG 3 cut(s) 748, 790, 877
TseFI GTSAC 2 cut(s) 77, 1222
TseI GCWGC 9 cut(s) 91, 392, 605, 896, 920, 1322, 1412, 1427, 1430
Tsp45I GTSAC 2 cut(s) 77, 1222
TspDTI ATGAA 6 cut(s) 83, 181, 452, 474, 1261, 1332
TspGWI ACGGA 3 cut(s) 821, 1134, 1300
TspMI CCCGGG 1 cut(s) 130
TspRI CASTG 3 cut(s) 748, 790, 877
VneI GTGCAC 1 cut(s) 1169
VpaK11BI GGWCC 2 cut(s) 620, 1160
XagI CCTNNNNNAGG 1 cut(s) 272
XcmI CCANNNNNNNNNTGG 2 cut(s) 254, 581
XmaI CCCGGG 1 cut(s) 130
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.