FvH4_2g29730

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010530
Physical Location & Seq
Reverse (-)
23006735 .. 23009672
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29730.t1

Sequence Viewer

Length: 432 bp
ATGTCTAGTCCTCTTCGGCTCGTCTTCCTTCCATGCCTGCTCCTCTTTGTCGTTCTCGCAGCTCTCTTCCGTTCATCTCAGGCCGATGTTGGTACCGCTGCACAATACAGACCACCTTATCTACCTACGTCCTGCTATGGTAGCGATGCGTCGCAGTTTCCGTCGAGCAACTTGTTCGGGTCAGCCGGAGAAGGAATATGGGACAACGGAGCAGCCTGTGGAAGGCAGTATCTGGTGAGGTGCATAAGCGCCGCTGTCCCGAAAACTTGCATTCCGGGGAAGACGATTCAGATCAAGATTGTGGATCGCGCTCTTTCTTCTGTGTCTAGGCCTTCGAGGAATGACGCTACCATGGTGCTGTCCACAACTGCATTTGCCGCCATCGCCAACTCTTCTGCTTCATTTGTTAACGTCGAATTCCAACAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

144

Amino Acids

15.17

Weight (kDa)

8.97

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 66 - 140 4.2e-10 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 92
AccB1I GGYRCC 1 cut(s) 92
AccII CGCG 1 cut(s) 309
AciI CCGC 3 cut(s) 96, 252, 378
AclWI GGATC 1 cut(s) 312
AcsI RAATTY 1 cut(s) 416
AfaI GTAC 1 cut(s) 94
AfiI CCNNNNNNNGG 1 cut(s) 222
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
AlwI GGATC 1 cut(s) 312
AlwNI CAGNNNCTG 1 cut(s) 232
AoxI GGCC 2 cut(s) 81, 329
ApeKI GCWGC 3 cut(s) 59, 98, 212
ApoI RAATTY 1 cut(s) 416
Asp718I GGTACC 1 cut(s) 92
AspLEI GCGC 2 cut(s) 251, 311
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 247
BanI GGYRCC 1 cut(s) 92
BbsI GAAGAC 2 cut(s) 16, 287
BbvI GCAGC 3 cut(s) 71, 85, 224
BccI CCATC 1 cut(s) 389
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BcnI CCSGG 1 cut(s) 276
BfaI CTAG 2 cut(s) 6, 327
BfoI RGCGCY 1 cut(s) 252
BisI GCNGC 5 cut(s) 60, 99, 213, 252, 378
BlsI GCNGC 5 cut(s) 61, 100, 214, 253, 379
Bme1390I CCNGG 1 cut(s) 276
BmiI GGNNCC 1 cut(s) 94
BmrFI CCNGG 1 cut(s) 276
BmsI GCATC 1 cut(s) 136
BpiI GAAGAC 2 cut(s) 16, 287
BpuMI CCSGG 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 275, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse8I GATNNNNATC 1 cut(s) 290
BseDI CCNNGG 2 cut(s) 275, 351
BseJI GATNNNNATC 1 cut(s) 290
BseLI CCNNNNNNNGG 1 cut(s) 222
BseMII CTCAG 1 cut(s) 92
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 3 cut(s) 71, 85, 224
BsgI GTGCAG 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 2 cut(s) 83, 331
BshNI GGYRCC 1 cut(s) 92
BsiSI CCGG 2 cut(s) 186, 275
BslFI GGGAC 2 cut(s) 215, 242
BslI CCNNNNNNNGG 1 cut(s) 222
BsmFI GGGAC 2 cut(s) 215, 242
BsmI GAATGC 1 cut(s) 270
BsnI GGCC 2 cut(s) 83, 331
Bsp143I GATC 2 cut(s) 291, 304
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 3 cut(s) 96, 252, 378
BspANI GGCC 2 cut(s) 83, 331
BspCNI CTCAG 1 cut(s) 91
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 94
BspPI GGATC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 275, 351
BssMI GATC 2 cut(s) 291, 304
BssT1I CCWWGG 1 cut(s) 351
Bst6I CTCTTC 3 cut(s) 18, 71, 397
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 78
BstDSI CCRYGG 1 cut(s) 351
BstENI CCTNNNNNAGG 1 cut(s) 220
BstFNI CGCG 1 cut(s) 309
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 2 cut(s) 251, 311
BstKTI GATC 2 cut(s) 294, 307
BstMBI GATC 2 cut(s) 291, 304
BstMWI GCNNNNNNNGC 3 cut(s) 141, 377, 383
BstSCI CCNGG 1 cut(s) 274
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 3 cut(s) 71, 85, 224
BstV2I GAAGAC 2 cut(s) 16, 287
BsuRI GGCC 2 cut(s) 83, 331
BtgI CCRYGG 1 cut(s) 351
BtgZI GCGATG 2 cut(s) 159, 367
Cac8I GCNNGC 1 cut(s) 38
CaiI CAGNNNCTG 1 cut(s) 232
CfoI GCGC 2 cut(s) 251, 311
CseI GACGC 2 cut(s) 138, 353
Csp6I GTAC 1 cut(s) 93
CviAII CATG 2 cut(s) 33, 352
CviJI RGCY 6 cut(s) 19, 62, 83, 185, 215, 331
CviKI_1 RGCY 6 cut(s) 19, 62, 83, 185, 215, 331
CviQI GTAC 1 cut(s) 93
DdeI CTNAG 1 cut(s) 78
DpnI GATC 2 cut(s) 293, 306
DpnII GATC 2 cut(s) 291, 304
Eam1104I CTCTTC 3 cut(s) 18, 71, 397
EarI CTCTTC 3 cut(s) 18, 71, 397
Eco130I CCWWGG 1 cut(s) 351
Eco147I AGGCCT 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 416
EcoT14I CCWWGG 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 2 cut(s) 36, 355
FaiI YATR 5 cut(s) 34, 138, 199, 245, 353
FaqI GGGAC 2 cut(s) 215, 242
FatI CATG 2 cut(s) 32, 351
Fnu4HI GCNGC 5 cut(s) 60, 99, 213, 252, 378
Fsp4HI GCNGC 5 cut(s) 60, 99, 213, 252, 378
FspBI CTAG 2 cut(s) 6, 327
GlaI GCGC 2 cut(s) 250, 310
GluI GCNGC 5 cut(s) 60, 99, 213, 252, 378
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 2 cut(s) 83, 331
HapII CCGG 2 cut(s) 186, 275
HgaI GACGC 2 cut(s) 138, 353
HhaI GCGC 2 cut(s) 251, 311
Hin1II CATG 2 cut(s) 36, 355
Hin6I GCGC 2 cut(s) 249, 309
HinP1I GCGC 2 cut(s) 249, 309
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 1 cut(s) 286
HpaI GTTAAC 1 cut(s) 409
HpaII CCGG 2 cut(s) 186, 275
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 2 cut(s) 363, 409
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 2 cut(s) 259, 295
Hpy8I GTNNAC 2 cut(s) 363, 409
Hpy99I CGWCG 3 cut(s) 154, 166, 416
HpyAV CCTTC 4 cut(s) 38, 185, 216, 342
HpyCH4IV ACGT 2 cut(s) 128, 411
HpyCH4V TGCA 4 cut(s) 101, 243, 270, 371
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 377, 383
HpyF3I CTNAG 1 cut(s) 78
HpySE526I ACGT 2 cut(s) 128, 411
Hsp92II CATG 2 cut(s) 36, 355
HspAI GCGC 2 cut(s) 249, 309
KpnI GGTACC 1 cut(s) 96
KspAI GTTAAC 1 cut(s) 409
Kzo9I GATC 2 cut(s) 291, 304
LmnI GCTCC 2 cut(s) 45, 209
LpnPI CCDG 8 cut(s) 50, 65, 145, 199, 218, 229, 288, 410
Lsp1109I GCAGC 3 cut(s) 71, 85, 224
LweI GCATC 1 cut(s) 136
MaeI CTAG 2 cut(s) 6, 327
MaeII ACGT 2 cut(s) 128, 411
MalI GATC 2 cut(s) 293, 306
MboI GATC 2 cut(s) 291, 304
MboII GAAGA 6 cut(s) 5, 16, 58, 292, 309, 384
MluCI AATT 1 cut(s) 416
MnlI CCTC 4 cut(s) 21, 53, 231, 330
MseI TTAA 1 cut(s) 408
MspA1I CMGCKG 2 cut(s) 98, 254
MspI CCGG 2 cut(s) 186, 275
MspR9I CCNGG 1 cut(s) 276
Mva1269I GAATGC 1 cut(s) 270
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 3 cut(s) 141, 377, 383
NciI CCSGG 1 cut(s) 276
NcoI CCATGG 1 cut(s) 351
NdeII GATC 2 cut(s) 291, 304
NlaIII CATG 2 cut(s) 36, 355
NlaIV GGNNCC 1 cut(s) 94
PceI AGGCCT 1 cut(s) 331
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PctI GAATGC 1 cut(s) 270
PfeI GAWTC 1 cut(s) 286
PkrI GCNGC 5 cut(s) 61, 100, 214, 253, 379
PspN4I GGNNCC 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 232
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 5 cut(s) 60, 99, 213, 252, 378
Sau3AI GATC 2 cut(s) 291, 304
ScrFI CCNGG 1 cut(s) 276
SetI ASST 7 cut(s) 64, 118, 127, 131, 242, 414, 429
SfaNI GCATC 1 cut(s) 136
Sse9I AATT 1 cut(s) 416
SseBI AGGCCT 1 cut(s) 331
SsiI CCGC 3 cut(s) 96, 252, 378
SspMI CTAG 2 cut(s) 6, 327
StuI AGGCCT 1 cut(s) 331
StyD4I CCNGG 1 cut(s) 274
StyI CCWWGG 1 cut(s) 351
TaiI ACGT 2 cut(s) 131, 414
TaqI TCGA 3 cut(s) 164, 335, 414
TasI AATT 1 cut(s) 416
TauI GCSGC 2 cut(s) 254, 380
TfiI GAWTC 1 cut(s) 286
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TseI GCWGC 3 cut(s) 59, 98, 212
TspDTI ATGAA 2 cut(s) 63, 390
TspGWI ACGGA 3 cut(s) 59, 150, 222
XagI CCTNNNNNAGG 1 cut(s) 220
XapI RAATTY 1 cut(s) 416
XspI CTAG 2 cut(s) 6, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.