MD15G1119000.v1.1

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010530
Physical Location & Seq
Reverse (-)
8546924 .. 8550038
3115 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1119000.v1.1.491

Sequence Viewer

Length: 546 bp
ATGTATTTCCGTCCTCCATCTTCTAATTGTATTAGTCGACCAGTTTTGTTAATTTGTCCTGCACTCGATCATTCTTCAGTGCCCTATTCTTCTCGCCGATCACATAATTACCAGGAATGTCTAAGCCTCAGAGTTCTCTTCCATACCTGCTTCTGTTCTTCTTCTCAGCAGCGCTCATTCATTCCTCTCAAGCTGATGTTGGCACTGCTGCTCAGTACAGGCCCCCTTATTTACGTAACTACCGCCTGCGGTGGAAGCGACCAGGGACAGTTTCCTCCGGGCAATCTGTTCGCGTCCGTTGGCGAGGGAATTTGGGACAACGGTGCAGCTTGTGGAAGGCAATATCAGGTGAGGTGCATAAGCGCTCCCGTGCGAGGAACCTGCGTTCCAGGGAACACGATTCAGGTAAAGATTGTGGATCGTGCACTCTCTTCGGTGTCCCGACCTTCAAGAAGTGATGCTACCATAGTTCTCTCTACTACTGCATTTGCTGCAATCGCCAACTCTTCCGCTACATTCGTTAACATCGAATTTCAACAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

182

Amino Acids

19.55

Weight (kDa)

8.98

Isoelectric Point (pI)

59.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 103 - 177 1.9e-10 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 155, 389
AccI GTMKAC 1 cut(s) 37
AccII CGCG 1 cut(s) 293
AciI CCGC 3 cut(s) 243, 249, 510
AclWI GGATC 1 cut(s) 426
AcsI RAATTY 2 cut(s) 309, 530
AcuI CTGAAG 1 cut(s) 60
AfaI GTAC 1 cut(s) 217
AfeI AGCGCT 2 cut(s) 173, 364
AfiI CCNNNNNNNGG 1 cut(s) 374
AgsI TTSAA 2 cut(s) 450, 536
AjnI CCWGG 3 cut(s) 111, 261, 388
AloI GAACNNNNNNTCC 4 cut(s) 369, 370, 401, 402
AluBI AGCT 2 cut(s) 193, 329
AluI AGCT 2 cut(s) 193, 329
Alw21I GWGCWC 1 cut(s) 427
Alw44I GTGCAC 1 cut(s) 423
AlwI GGATC 1 cut(s) 426
Aor51HI AGCGCT 2 cut(s) 173, 364
AoxI GGCC 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 423
ApeKI GCWGC 4 cut(s) 169, 208, 326, 491
ApoI RAATTY 2 cut(s) 309, 530
AspLEI GCGC 2 cut(s) 174, 365
AspS9I GGNCC 1 cut(s) 221
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 1 cut(s) 361
BaeGI GKGCMC 2 cut(s) 84, 427
BarI GAAGNNNNNNTAC 2 cut(s) 445, 477
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 4 cut(s) 181, 195, 338, 478
BccI CCATC 1 cut(s) 25
BcgI CGANNNNNNTGC 6 cut(s) 271, 305, 363, 397, 414, 448
BciT130I CCWGG 3 cut(s) 113, 263, 390
BcnI CCSGG 1 cut(s) 279
BfoI RGCGCY 2 cut(s) 175, 366
BfuAI ACCTGC 2 cut(s) 155, 389
BisI GCNGC 4 cut(s) 170, 209, 327, 492
BlsI GCNGC 4 cut(s) 171, 210, 328, 493
Bme1390I CCNGG 4 cut(s) 113, 263, 279, 390
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 2 cut(s) 223, 379
BmrFI CCNGG 4 cut(s) 113, 263, 279, 390
BmsI GCATC 1 cut(s) 448
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 279
BsaAI YACGTR 1 cut(s) 235
BsaJI CCNNGG 2 cut(s) 262, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse1I ACTGG 1 cut(s) 41
BseBI CCWGG 3 cut(s) 113, 263, 390
BseDI CCNNGG 2 cut(s) 262, 389
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMII CTCAG 3 cut(s) 142, 179, 226
BseNI ACTGG 1 cut(s) 41
BseSI GKGCMC 2 cut(s) 84, 427
BseXI GCAGC 4 cut(s) 181, 195, 338, 478
BsgI GTGCAG 2 cut(s) 45, 345
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 427
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 3 cut(s) 279, 329, 424
BslI CCNNNNNNNGG 1 cut(s) 374
BsmFI GGGAC 3 cut(s) 279, 329, 424
BsnI GGCC 1 cut(s) 222
Bsp1286I GDGCHC 2 cut(s) 84, 427
Bsp143I GATC 3 cut(s) 67, 98, 418
BspACI CCGC 3 cut(s) 243, 249, 510
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 3 cut(s) 141, 178, 225
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 2 cut(s) 223, 379
BspMI ACCTGC 2 cut(s) 155, 389
BspPI GGATC 1 cut(s) 426
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 262, 389
BssMI GATC 3 cut(s) 67, 98, 418
Bst2UI CCWGG 3 cut(s) 113, 263, 390
Bst4CI ACNGT 2 cut(s) 270, 323
Bst6I CTCTTC 3 cut(s) 143, 436, 511
BstAPI GCANNNNNTGC 1 cut(s) 491
BstBAI YACGTR 1 cut(s) 235
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 4 cut(s) 122, 128, 165, 212
BstFNI CGCG 1 cut(s) 293
BstH2I RGCGCY 2 cut(s) 175, 366
BstHHI GCGC 2 cut(s) 174, 365
BstKTI GATC 3 cut(s) 70, 101, 421
BstMBI GATC 3 cut(s) 67, 98, 418
BstMWI GCNNNNNNNGC 3 cut(s) 255, 491, 497
BstNI CCWGG 3 cut(s) 113, 263, 390
BstSCI CCNGG 4 cut(s) 111, 261, 277, 388
BstSLI GKGCMC 2 cut(s) 84, 427
BstSNI TACGTA 1 cut(s) 235
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 4 cut(s) 181, 195, 338, 478
BsuRI GGCC 1 cut(s) 222
BtsI GCAGTG 1 cut(s) 203
BtsIMutI CAGTG 2 cut(s) 84, 203
BveI ACCTGC 2 cut(s) 155, 389
Cac8I GCNNGC 1 cut(s) 247
CfoI GCGC 2 cut(s) 174, 365
Cfr13I GGNCC 1 cut(s) 221
CseI GACGC 1 cut(s) 282
Csp6I GTAC 1 cut(s) 216
CviJI RGCY 4 cut(s) 126, 193, 222, 329
CviKI_1 RGCY 4 cut(s) 126, 193, 222, 329
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 4 cut(s) 122, 128, 165, 212
DpnI GATC 3 cut(s) 69, 100, 420
DpnII GATC 3 cut(s) 67, 98, 418
Eam1104I CTCTTC 3 cut(s) 143, 436, 511
EarI CTCTTC 3 cut(s) 143, 436, 511
Eco105I TACGTA 1 cut(s) 235
Eco47III AGCGCT 2 cut(s) 173, 364
Eco57I CTGAAG 1 cut(s) 60
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 3 cut(s) 111, 261, 388
FaiI YATR 4 cut(s) 105, 144, 359, 467
FaqI GGGAC 3 cut(s) 279, 329, 424
FblI GTMKAC 1 cut(s) 37
Fnu4HI GCNGC 4 cut(s) 170, 209, 327, 492
Fsp4HI GCNGC 4 cut(s) 170, 209, 327, 492
GlaI GCGC 2 cut(s) 173, 364
GluI GCNGC 4 cut(s) 170, 209, 327, 492
HaeII RGCGCY 2 cut(s) 175, 366
HaeIII GGCC 1 cut(s) 222
HapII CCGG 1 cut(s) 278
HgaI GACGC 1 cut(s) 282
HhaI GCGC 2 cut(s) 174, 365
Hin6I GCGC 2 cut(s) 172, 363
HinP1I GCGC 2 cut(s) 172, 363
HincII GTYRAC 2 cut(s) 38, 523
HindII GTYRAC 2 cut(s) 38, 523
HinfI GANTC 1 cut(s) 400
HpaI GTTAAC 1 cut(s) 523
HpaII CCGG 1 cut(s) 278
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 3 cut(s) 38, 425, 523
Hpy188I TCNGA 1 cut(s) 131
Hpy188III TCNNGA 2 cut(s) 441, 450
Hpy8I GTNNAC 3 cut(s) 38, 425, 523
HpyAV CCTTC 2 cut(s) 330, 456
HpyCH4III ACNGT 2 cut(s) 270, 323
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 6 cut(s) 62, 326, 357, 425, 485, 494
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 491, 497
HpyF3I CTNAG 4 cut(s) 122, 128, 165, 212
HpySE526I ACGT 1 cut(s) 234
HspAI GCGC 2 cut(s) 172, 363
KspAI GTTAAC 1 cut(s) 523
Kzo9I GATC 3 cut(s) 67, 98, 418
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 4 cut(s) 181, 195, 338, 478
LweI GCATC 1 cut(s) 448
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 235
MalI GATC 3 cut(s) 69, 100, 420
MboI GATC 3 cut(s) 67, 98, 418
MboII GAAGA 8 cut(s) 12, 66, 81, 130, 150, 153, 423, 498
MhlI GDGCHC 2 cut(s) 84, 427
MluCI AATT 5 cut(s) 25, 51, 106, 309, 530
MnlI CCTC 7 cut(s) 24, 137, 195, 285, 298, 345, 368
MseI TTAA 3 cut(s) 50, 522, 544
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 4 cut(s) 113, 263, 279, 390
MvaI CCWGG 3 cut(s) 113, 263, 390
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 3 cut(s) 255, 491, 497
NciI CCSGG 1 cut(s) 279
NdeII GATC 3 cut(s) 67, 98, 418
NlaIV GGNNCC 2 cut(s) 223, 379
PcsI WCGNNNNNNNCGW 1 cut(s) 525
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 4 cut(s) 171, 210, 328, 493
Ppu21I YACGTR 1 cut(s) 235
Psp6I CCWGG 3 cut(s) 111, 261, 388
PspGI CCWGG 3 cut(s) 111, 261, 388
PspN4I GGNNCC 2 cut(s) 223, 379
PspPI GGNCC 1 cut(s) 221
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
SalI GTCGAC 1 cut(s) 36
SaqAI TTAA 3 cut(s) 50, 522, 544
SatI GCNGC 4 cut(s) 170, 209, 327, 492
Sau3AI GATC 3 cut(s) 67, 98, 418
Sau96I GGNCC 1 cut(s) 221
ScrFI CCNGG 4 cut(s) 113, 263, 279, 390
SduI GDGCHC 2 cut(s) 84, 427
SetI ASST 9 cut(s) 149, 195, 237, 331, 351, 356, 383, 408, 448
SfaNI GCATC 1 cut(s) 448
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SnaBI TACGTA 1 cut(s) 235
Sse9I AATT 5 cut(s) 25, 51, 106, 309, 530
SsiI CCGC 3 cut(s) 243, 249, 510
StyD4I CCNGG 4 cut(s) 111, 261, 277, 388
TaaI ACNGT 2 cut(s) 270, 323
TaiI ACGT 1 cut(s) 237
TaqI TCGA 3 cut(s) 37, 66, 528
TasI AATT 5 cut(s) 25, 51, 106, 309, 530
TatI WGTACW 1 cut(s) 215
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 3 cut(s) 50, 522, 544
Tru9I TTAA 3 cut(s) 50, 522, 544
TscAI CASTG 2 cut(s) 84, 210
TseI GCWGC 4 cut(s) 169, 208, 326, 491
TspDTI ATGAA 1 cut(s) 169
TspGWI ACGGA 1 cut(s) 286
TspRI CASTG 2 cut(s) 84, 210
VneI GTGCAC 1 cut(s) 423
XapI RAATTY 2 cut(s) 309, 530
XmiI GTMKAC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.