MD08G1142500.v1.1

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010530
Physical Location & Seq
Reverse (-)
14123385 .. 14124253
869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1142500.v1.1.491

Sequence Viewer

Length: 423 bp
ATGTCTAAGCCTCAGAGTTCTCTTCCATGCCTGCTTCTGATCTTCTTCTCCGCAGCGCTCCTTCATTCCTCTCAGGCTGATGTTGGCACTGCTGCTCAGTACAGGCCCCCTTATTTACCTACCGCCTGTGGAGGAAGTGACCAGGGACAGTTTCCTCCGGGCAACCTGTTCGTGTCCGTTGGCGAGGGGATTTGGGACAATGGTGCAGCTTGTGGAAGGCAATATCAGGTGAGGTGCATAAGCGCTCCCGTGCGAGAAACCTGCGTTCCAGGGAACACGATTCAGGTAAAGATTGTGGATCGTGCACTCTCTTCGGTGTCCCGACCTTCAAGAAGTGATGCTACCATAGTTCTCTCTACTACTGCATTTGCTGCAATCGCCAACTCTTCCGCTACATTCGTTAACATAGAATTTCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

141

Amino Acids

14.77

Weight (kDa)

7.62

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 64 - 138 8.3e-11 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 269
AciI CCGC 3 cut(s) 51, 123, 390
AclWI GGATC 1 cut(s) 306
AcsI RAATTY 1 cut(s) 410
AfaI GTAC 1 cut(s) 101
AfeI AGCGCT 2 cut(s) 57, 244
AgsI TTSAA 2 cut(s) 330, 416
AjnI CCWGG 2 cut(s) 141, 268
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw21I GWGCWC 1 cut(s) 307
Alw44I GTGCAC 1 cut(s) 303
AlwI GGATC 1 cut(s) 306
Aor51HI AGCGCT 2 cut(s) 57, 244
AoxI GGCC 1 cut(s) 104
ApaLI GTGCAC 1 cut(s) 303
ApeKI GCWGC 4 cut(s) 53, 92, 206, 371
ApoI RAATTY 1 cut(s) 410
AspLEI GCGC 2 cut(s) 58, 245
AspS9I GGNCC 1 cut(s) 105
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 1 cut(s) 241
BaeGI GKGCMC 1 cut(s) 307
BarI GAAGNNNNNNTAC 2 cut(s) 325, 357
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 4 cut(s) 65, 79, 218, 358
BcgI CGANNNNNNTGC 6 cut(s) 151, 185, 243, 277, 294, 328
BciT130I CCWGG 2 cut(s) 143, 270
BcnI CCSGG 1 cut(s) 159
BfoI RGCGCY 2 cut(s) 59, 246
BfuAI ACCTGC 1 cut(s) 269
BisI GCNGC 4 cut(s) 54, 93, 207, 372
BlsI GCNGC 4 cut(s) 55, 94, 208, 373
Bme1390I CCNGG 3 cut(s) 143, 159, 270
BmgT120I GGNCC 1 cut(s) 105
BmiI GGNNCC 1 cut(s) 107
BmrFI CCNGG 3 cut(s) 143, 159, 270
BmsI GCATC 1 cut(s) 328
BpuMI CCSGG 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 142, 269
BseBI CCWGG 2 cut(s) 143, 270
BseDI CCNNGG 2 cut(s) 142, 269
BseMII CTCAG 3 cut(s) 26, 86, 110
BseSI GKGCMC 1 cut(s) 307
BseXI GCAGC 4 cut(s) 65, 79, 218, 358
BsgI GTGCAG 1 cut(s) 225
BshFI GGCC 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 3 cut(s) 159, 209, 304
BsmFI GGGAC 3 cut(s) 159, 209, 304
BsnI GGCC 1 cut(s) 106
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 2 cut(s) 39, 298
BspACI CCGC 3 cut(s) 51, 123, 390
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 3 cut(s) 25, 85, 109
BspLI GGNNCC 1 cut(s) 107
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 1 cut(s) 306
BssECI CCNNGG 2 cut(s) 142, 269
BssMI GATC 2 cut(s) 39, 298
Bst2UI CCWGG 2 cut(s) 143, 270
Bst4CI ACNGT 2 cut(s) 150, 420
Bst6I CTCTTC 3 cut(s) 27, 316, 391
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 4 cut(s) 6, 12, 72, 96
BstH2I RGCGCY 2 cut(s) 59, 246
BstHHI GCGC 2 cut(s) 58, 245
BstKTI GATC 2 cut(s) 42, 301
BstMBI GATC 2 cut(s) 39, 298
BstMWI GCNNNNNNNGC 2 cut(s) 371, 377
BstNI CCWGG 2 cut(s) 143, 270
BstSCI CCNGG 3 cut(s) 141, 157, 268
BstSLI GKGCMC 1 cut(s) 307
BstV1I GCAGC 4 cut(s) 65, 79, 218, 358
BsuRI GGCC 1 cut(s) 106
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 87
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 1 cut(s) 32
CfoI GCGC 2 cut(s) 58, 245
Cfr13I GGNCC 1 cut(s) 105
Csp6I GTAC 1 cut(s) 100
CviAII CATG 1 cut(s) 27
CviJI RGCY 4 cut(s) 10, 77, 106, 209
CviKI_1 RGCY 4 cut(s) 10, 77, 106, 209
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 4 cut(s) 6, 12, 72, 96
DpnI GATC 2 cut(s) 41, 300
DpnII GATC 2 cut(s) 39, 298
Eam1104I CTCTTC 3 cut(s) 27, 316, 391
EarI CTCTTC 3 cut(s) 27, 316, 391
Eco47III AGCGCT 2 cut(s) 57, 244
EcoO109I RGGNCCY 1 cut(s) 105
EcoRII CCWGG 2 cut(s) 141, 268
FaeI CATG 1 cut(s) 30
FaiI YATR 4 cut(s) 28, 239, 347, 407
FaqI GGGAC 3 cut(s) 159, 209, 304
FatI CATG 1 cut(s) 26
Fnu4HI GCNGC 4 cut(s) 54, 93, 207, 372
Fsp4HI GCNGC 4 cut(s) 54, 93, 207, 372
GlaI GCGC 2 cut(s) 57, 244
GluI GCNGC 4 cut(s) 54, 93, 207, 372
HaeII RGCGCY 2 cut(s) 59, 246
HaeIII GGCC 1 cut(s) 106
HapII CCGG 1 cut(s) 158
HhaI GCGC 2 cut(s) 58, 245
Hin1II CATG 1 cut(s) 30
Hin6I GCGC 2 cut(s) 56, 243
HinP1I GCGC 2 cut(s) 56, 243
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 1 cut(s) 280
HpaI GTTAAC 1 cut(s) 403
HpaII CCGG 1 cut(s) 158
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 2 cut(s) 305, 403
Hpy188I TCNGA 2 cut(s) 15, 39
Hpy188III TCNNGA 2 cut(s) 321, 330
Hpy8I GTNNAC 2 cut(s) 305, 403
HpyAV CCTTC 3 cut(s) 71, 210, 336
HpyCH4III ACNGT 2 cut(s) 150, 420
HpyCH4V TGCA 5 cut(s) 206, 237, 305, 365, 374
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 377
HpyF3I CTNAG 4 cut(s) 6, 12, 72, 96
Hsp92II CATG 1 cut(s) 30
HspAI GCGC 2 cut(s) 56, 243
KspAI GTTAAC 1 cut(s) 403
Kzo9I GATC 2 cut(s) 39, 298
LmnI GCTCC 2 cut(s) 63, 250
Lsp1109I GCAGC 4 cut(s) 65, 79, 218, 358
LweI GCATC 1 cut(s) 328
MaeIII GTNAC 1 cut(s) 137
MalI GATC 2 cut(s) 41, 300
MboI GATC 2 cut(s) 39, 298
MboII GAAGA 5 cut(s) 14, 34, 37, 303, 378
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 1 cut(s) 410
MnlI CCTC 6 cut(s) 21, 79, 125, 165, 178, 225
MseI TTAA 1 cut(s) 402
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 3 cut(s) 143, 159, 270
MvaI CCWGG 2 cut(s) 143, 270
MwoI GCNNNNNNNGC 2 cut(s) 371, 377
NciI CCSGG 1 cut(s) 159
NdeII GATC 2 cut(s) 39, 298
NlaIII CATG 1 cut(s) 30
NlaIV GGNNCC 1 cut(s) 107
NmuCI GTSAC 1 cut(s) 137
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 4 cut(s) 55, 94, 208, 373
Psp6I CCWGG 2 cut(s) 141, 268
PspGI CCWGG 2 cut(s) 141, 268
PspN4I GGNNCC 1 cut(s) 107
PspPI GGNCC 1 cut(s) 105
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 4 cut(s) 54, 93, 207, 372
Sau3AI GATC 2 cut(s) 39, 298
Sau96I GGNCC 1 cut(s) 105
ScrFI CCNGG 3 cut(s) 143, 159, 270
SduI GDGCHC 1 cut(s) 307
SetI ASST 8 cut(s) 121, 168, 211, 231, 236, 263, 288, 328
SfaNI GCATC 1 cut(s) 328
Sse9I AATT 1 cut(s) 410
SsiI CCGC 3 cut(s) 51, 123, 390
StyD4I CCNGG 3 cut(s) 141, 157, 268
TaaI ACNGT 2 cut(s) 150, 420
TasI AATT 1 cut(s) 410
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 4 cut(s) 53, 92, 206, 371
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 1 cut(s) 53
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 1 cut(s) 94
VneI GTGCAC 1 cut(s) 303
XapI RAATTY 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.