Rroxscaffold_7G00169370

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010530
Physical Location & Seq
Reverse (-)
10023570 .. 10026750
3181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00169370.1

Sequence Viewer

Length: 420 bp
ATGTCTAGTCCTCTTCGGTTCCTTCCATTCCTGCTCTTCTTCGTTCTCGCAACTCTCTTCGGTCCCTCTCAGGCCGATGTTGGCACCGCTGCCCACTACAGCCCACCTTATCTGCCTACGTCCTGCTTTGGTAGCGATCCGTCGCAGTTTCCTTCAAGCAACCTGTTTGGGTCAGCCGGAGAAGGAATATGGGACAACGGAGCAGCCTGTGGCAGGCAGTATCTGGTGAGGTGCATAAGCGCCGCTGTCCCGAAAACTTGCATTCCGGGACAGACAATTCAGATCAAGATTGTGGATCGCGCGCTTTCTTCTAAGTCTAGGCCTTCGAGGAATGATGCTACCATGGTGCTTTCCACAACTGCATTTGCCGCCATCGCCAACTCTTCTGCTTCATTTGTTAACGTTGAATTTCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

139

Amino Acids

14.76

Weight (kDa)

8.45

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 63 - 137 5.6e-10 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AccII CGCG 2 cut(s) 300, 302
AciI CCGC 3 cut(s) 87, 243, 369
AclI AACGTT 1 cut(s) 402
AclWI GGATC 2 cut(s) 131, 303
AcsI RAATTY 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 213
AgsI TTSAA 3 cut(s) 156, 407, 413
AlwI GGATC 2 cut(s) 131, 303
AlwNI CAGNNNCTG 1 cut(s) 223
AoxI GGCC 2 cut(s) 72, 320
ApeKI GCWGC 2 cut(s) 89, 203
ApoI RAATTY 1 cut(s) 407
AspLEI GCGC 3 cut(s) 242, 302, 304
AspS9I GGNCC 1 cut(s) 62
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 1 cut(s) 238
AvaII GGWCC 1 cut(s) 62
BanI GGYRCC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 76, 215
BccI CCATC 1 cut(s) 380
BcnI CCSGG 1 cut(s) 267
BfaI CTAG 2 cut(s) 6, 318
BfmI CTRYAG 1 cut(s) 97
BfoI RGCGCY 1 cut(s) 243
BisI GCNGC 4 cut(s) 90, 204, 243, 369
BlsI GCNGC 4 cut(s) 91, 205, 244, 370
Bme1390I CCNGG 1 cut(s) 267
Bme18I GGWCC 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 3 cut(s) 20, 64, 85
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 1 cut(s) 325
BpuMI CCSGG 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 342
Bsc4I CCNNNNNNNGG 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 342
BseLI CCNNNNNNNGG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 83
BsePI GCGCGC 1 cut(s) 300
BseXI GCAGC 2 cut(s) 76, 215
Bsh1236I CGCG 2 cut(s) 300, 302
BshFI GGCC 2 cut(s) 74, 322
BshNI GGYRCC 1 cut(s) 83
BsiSI CCGG 2 cut(s) 177, 266
BslFI GGGAC 4 cut(s) 48, 206, 233, 282
BslI CCNNNNNNNGG 1 cut(s) 213
BsmFI GGGAC 4 cut(s) 48, 206, 233, 282
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 2 cut(s) 74, 322
Bsp143I GATC 3 cut(s) 136, 282, 295
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 3 cut(s) 87, 243, 369
BspANI GGCC 2 cut(s) 74, 322
BspCNI CTCAG 1 cut(s) 82
BspFNI CGCG 2 cut(s) 300, 302
BspLI GGNNCC 3 cut(s) 20, 64, 85
BspPI GGATC 2 cut(s) 131, 303
BspQI GCTCTTC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 342
BssHII GCGCGC 1 cut(s) 300
BssMI GATC 3 cut(s) 136, 282, 295
BssT1I CCWWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 417
Bst6I CTCTTC 4 cut(s) 18, 41, 62, 388
BstC8I GCNNGC 2 cut(s) 215, 302
BstDEI CTNAG 2 cut(s) 69, 312
BstDSI CCRYGG 1 cut(s) 342
BstENI CCTNNNNNAGG 1 cut(s) 211
BstFNI CGCG 2 cut(s) 300, 302
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 3 cut(s) 242, 302, 304
BstKTI GATC 3 cut(s) 139, 285, 298
BstMBI GATC 3 cut(s) 136, 282, 295
BstMWI GCNNNNNNNGC 3 cut(s) 132, 368, 374
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 97
BstUI CGCG 2 cut(s) 300, 302
BstV1I GCAGC 2 cut(s) 76, 215
BsuRI GGCC 2 cut(s) 74, 322
BtgI CCRYGG 1 cut(s) 342
BtgZI GCGATG 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 215, 302
CaiI CAGNNNCTG 1 cut(s) 223
CfoI GCGC 3 cut(s) 242, 302, 304
Cfr13I GGNCC 1 cut(s) 62
CviAII CATG 1 cut(s) 343
CviJI RGCY 5 cut(s) 74, 102, 176, 206, 322
CviKI_1 RGCY 5 cut(s) 74, 102, 176, 206, 322
DdeI CTNAG 2 cut(s) 69, 312
DpnI GATC 3 cut(s) 138, 284, 297
DpnII GATC 3 cut(s) 136, 282, 295
Eam1104I CTCTTC 4 cut(s) 18, 41, 62, 388
EarI CTCTTC 4 cut(s) 18, 41, 62, 388
Eco130I CCWWGG 1 cut(s) 342
Eco147I AGGCCT 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 62
EcoNI CCTNNNNNAGG 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 1 cut(s) 346
FaiI YATR 3 cut(s) 190, 236, 344
FaqI GGGAC 4 cut(s) 48, 206, 233, 282
FatI CATG 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 90, 204, 243, 369
Fsp4HI GCNGC 4 cut(s) 90, 204, 243, 369
FspBI CTAG 2 cut(s) 6, 318
GlaI GCGC 3 cut(s) 241, 301, 303
GluI GCNGC 4 cut(s) 90, 204, 243, 369
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 2 cut(s) 74, 322
HapII CCGG 2 cut(s) 177, 266
HhaI GCGC 3 cut(s) 242, 302, 304
Hin1II CATG 1 cut(s) 346
Hin6I GCGC 3 cut(s) 240, 300, 302
HinP1I GCGC 3 cut(s) 240, 300, 302
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HpaI GTTAAC 1 cut(s) 400
HpaII CCGG 2 cut(s) 177, 266
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 1 cut(s) 282
Hpy188III TCNNGA 2 cut(s) 250, 286
Hpy8I GTNNAC 1 cut(s) 400
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 4 cut(s) 32, 162, 176, 333
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4IV ACGT 2 cut(s) 119, 402
HpyCH4V TGCA 3 cut(s) 234, 261, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 368, 374
HpyF3I CTNAG 2 cut(s) 69, 312
HpySE526I ACGT 2 cut(s) 119, 402
Hsp92II CATG 1 cut(s) 346
HspAI GCGC 3 cut(s) 240, 300, 302
KspAI GTTAAC 1 cut(s) 400
Kzo9I GATC 3 cut(s) 136, 282, 295
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 1 cut(s) 200
LpnPI CCDG 9 cut(s) 44, 56, 136, 176, 190, 199, 209, 220, 279
Lsp1109I GCAGC 2 cut(s) 76, 215
LweI GCATC 1 cut(s) 325
MaeI CTAG 2 cut(s) 6, 318
MaeII ACGT 2 cut(s) 119, 402
MalI GATC 3 cut(s) 138, 284, 297
MboI GATC 3 cut(s) 136, 282, 295
MboII GAAGA 6 cut(s) 5, 28, 31, 49, 300, 375
MluCI AATT 2 cut(s) 276, 407
MnlI CCTC 4 cut(s) 21, 76, 222, 321
MseI TTAA 1 cut(s) 399
MspA1I CMGCKG 2 cut(s) 89, 245
MspI CCGG 2 cut(s) 177, 266
MspR9I CCNGG 1 cut(s) 267
Mva1269I GAATGC 1 cut(s) 261
MvnI CGCG 2 cut(s) 300, 302
MwoI GCNNNNNNNGC 3 cut(s) 132, 368, 374
NciI CCSGG 1 cut(s) 267
NcoI CCATGG 1 cut(s) 342
NdeII GATC 3 cut(s) 136, 282, 295
NlaIII CATG 1 cut(s) 346
NlaIV GGNNCC 3 cut(s) 20, 64, 85
PauI GCGCGC 1 cut(s) 300
PceI AGGCCT 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 41
PctI GAATGC 1 cut(s) 261
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 4 cut(s) 91, 205, 244, 370
Psp1406I AACGTT 1 cut(s) 402
PspN4I GGNNCC 3 cut(s) 20, 64, 85
PspPI GGNCC 1 cut(s) 62
PstNI CAGNNNCTG 1 cut(s) 223
PteI GCGCGC 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 4 cut(s) 90, 204, 243, 369
Sau3AI GATC 3 cut(s) 136, 282, 295
Sau96I GGNCC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 267
SetI ASST 5 cut(s) 109, 122, 165, 233, 405
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 97
SinI GGWCC 1 cut(s) 62
Sse9I AATT 2 cut(s) 276, 407
SseBI AGGCCT 1 cut(s) 322
SsiI CCGC 3 cut(s) 87, 243, 369
SspMI CTAG 2 cut(s) 6, 318
StuI AGGCCT 1 cut(s) 322
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 1 cut(s) 417
TaiI ACGT 2 cut(s) 122, 405
TaqI TCGA 1 cut(s) 326
TaqII GACCGA 1 cut(s) 50
TasI AATT 2 cut(s) 276, 407
TauI GCSGC 2 cut(s) 245, 371
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TseI GCWGC 2 cut(s) 89, 203
TspDTI ATGAA 1 cut(s) 381
TspGWI ACGGA 2 cut(s) 129, 213
VpaK11BI GGWCC 1 cut(s) 62
XagI CCTNNNNNAGG 1 cut(s) 211
XapI RAATTY 1 cut(s) 407
XspI CTAG 2 cut(s) 6, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.