RchiOBHm_Chr6g0298751

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010530
Physical Location & Seq
Forward (+)
60039270 .. 60049109
9840 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26821

Sequence Viewer

Length: 540 bp
ATGAACTACAACACGAATGAGCAGAGGAGAATTGCATCTGTTTGGAACTTTGTTACGATAATACCAAAACCATTGAACCTAGCTCAACCATCGATCTCCTCAAATCTAGCTAGCAGTATGTCTAGTCCTCTTCGGCTCCTTCCATTCCTGCTCTTCTTCGTTCTCGCAACTCTCTTCGGTCCCTCTCAGGCCGATGTTGGCACCGCTGCCCACTACAGCCCACCGTATCTGCCTACGTCCTGCTTTGGTAGCGATCCGTCGCAGTTTCCTTCAAGCAACCTGTTTGGGTCAGCCGGAGAAGGAATATGGGACAACGGAGCAGCCTGTGGCAGGCAGTATCTGGTGAGGTGCATAAGCGCCGCTGTCCCGAAAACTTGCATTCCGGGGCAGACGATTCAGATCAAGATTGTGGATCGCGCGCTTTCTTCTAAGTCTAGGCCTTCGAGGAATGATGCTACCATGGTGCTTTCCACAACTGCATTTGCCGCCATCGCCAACTCTTCTGCTTCATTTGTTAACGTTGAATTTCAACAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

179

Amino Acids

19.18

Weight (kDa)

9.07

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 102 - 176 1.1e-09 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 200
AccII CGCG 2 cut(s) 417, 419
AciI CCGC 3 cut(s) 204, 360, 486
AclI AACGTT 1 cut(s) 519
AclWI GGATC 2 cut(s) 248, 420
AcsI RAATTY 1 cut(s) 524
AfiI CCNNNNNNNGG 1 cut(s) 330
AgsI TTSAA 4 cut(s) 76, 273, 524, 530
AluBI AGCT 2 cut(s) 83, 110
AluI AGCT 2 cut(s) 83, 110
AlwI GGATC 2 cut(s) 248, 420
AlwNI CAGNNNCTG 1 cut(s) 340
AoxI GGCC 2 cut(s) 189, 437
ApeKI GCWGC 2 cut(s) 206, 320
ApoI RAATTY 1 cut(s) 524
AspLEI GCGC 3 cut(s) 359, 419, 421
AspS9I GGNCC 1 cut(s) 179
AsuC2I CCSGG 1 cut(s) 384
AsuHPI GGTGA 1 cut(s) 355
AsuNHI GCTAGC 1 cut(s) 110
AvaII GGWCC 1 cut(s) 179
BanI GGYRCC 1 cut(s) 200
BbvI GCAGC 2 cut(s) 193, 332
BccI CCATC 2 cut(s) 97, 497
BcnI CCSGG 1 cut(s) 384
BfaI CTAG 5 cut(s) 80, 107, 111, 123, 435
BfmI CTRYAG 1 cut(s) 214
BfoI RGCGCY 1 cut(s) 360
BisI GCNGC 4 cut(s) 207, 321, 360, 486
BlsI GCNGC 4 cut(s) 208, 322, 361, 487
Bme1390I CCNGG 1 cut(s) 384
Bme18I GGWCC 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 179
BmiI GGNNCC 3 cut(s) 137, 181, 202
BmrFI CCNGG 1 cut(s) 384
BmsI GCATC 2 cut(s) 44, 442
BmtI GCTAGC 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 384
Bsa29I ATCGAT 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 398
BsaJI CCNNGG 2 cut(s) 383, 459
Bsc4I CCNNNNNNNGG 1 cut(s) 330
Bse8I GATNNNNATC 1 cut(s) 398
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 2 cut(s) 383, 459
BseJI GATNNNNATC 1 cut(s) 398
BseLI CCNNNNNNNGG 1 cut(s) 330
BseMII CTCAG 1 cut(s) 200
BsePI GCGCGC 1 cut(s) 417
BseRI GAGGAG 2 cut(s) 40, 88
BseXI GCAGC 2 cut(s) 193, 332
Bsh1236I CGCG 2 cut(s) 417, 419
BshFI GGCC 2 cut(s) 191, 439
BshNI GGYRCC 1 cut(s) 200
BshVI ATCGAT 1 cut(s) 92
BsiSI CCGG 2 cut(s) 294, 383
BslFI GGGAC 3 cut(s) 165, 323, 350
BslI CCNNNNNNNGG 1 cut(s) 330
BsmFI GGGAC 3 cut(s) 165, 323, 350
BsmI GAATGC 1 cut(s) 378
BsnI GGCC 2 cut(s) 191, 439
Bsp143I GATC 4 cut(s) 93, 253, 399, 412
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 3 cut(s) 204, 360, 486
BspANI GGCC 2 cut(s) 191, 439
BspCNI CTCAG 1 cut(s) 199
BspDI ATCGAT 1 cut(s) 92
BspFNI CGCG 2 cut(s) 417, 419
BspLI GGNNCC 3 cut(s) 137, 181, 202
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 2 cut(s) 248, 420
BspQI GCTCTTC 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 200
BssECI CCNNGG 2 cut(s) 383, 459
BssHII GCGCGC 1 cut(s) 417
BssMI GATC 4 cut(s) 93, 253, 399, 412
BssT1I CCWWGG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 225
Bst6I CTCTTC 4 cut(s) 135, 158, 179, 505
BstC8I GCNNGC 3 cut(s) 112, 332, 419
BstDEI CTNAG 2 cut(s) 186, 429
BstDSI CCRYGG 1 cut(s) 459
BstENI CCTNNNNNAGG 1 cut(s) 328
BstFNI CGCG 2 cut(s) 417, 419
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 3 cut(s) 359, 419, 421
BstKTI GATC 4 cut(s) 96, 256, 402, 415
BstMBI GATC 4 cut(s) 93, 253, 399, 412
BstMWI GCNNNNNNNGC 3 cut(s) 249, 485, 491
BstSCI CCNGG 1 cut(s) 382
BstSFI CTRYAG 1 cut(s) 214
BstUI CGCG 2 cut(s) 417, 419
BstV1I GCAGC 2 cut(s) 193, 332
Bsu15I ATCGAT 1 cut(s) 92
BsuRI GGCC 2 cut(s) 191, 439
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 475
Cac8I GCNNGC 3 cut(s) 112, 332, 419
CaiI CAGNNNCTG 1 cut(s) 340
CfoI GCGC 3 cut(s) 359, 419, 421
Cfr13I GGNCC 1 cut(s) 179
ClaI ATCGAT 1 cut(s) 92
CviAII CATG 1 cut(s) 460
CviJI RGCY 8 cut(s) 83, 110, 136, 191, 219, 293, 323, 439
CviKI_1 RGCY 8 cut(s) 83, 110, 136, 191, 219, 293, 323, 439
DdeI CTNAG 2 cut(s) 186, 429
DpnI GATC 4 cut(s) 95, 255, 401, 414
DpnII GATC 4 cut(s) 93, 253, 399, 412
Eam1104I CTCTTC 4 cut(s) 135, 158, 179, 505
EarI CTCTTC 4 cut(s) 135, 158, 179, 505
Eco130I CCWWGG 1 cut(s) 459
Eco147I AGGCCT 1 cut(s) 439
Eco47I GGWCC 1 cut(s) 179
EcoNI CCTNNNNNAGG 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 1 cut(s) 463
FaiI YATR 4 cut(s) 119, 307, 353, 461
FaqI GGGAC 3 cut(s) 165, 323, 350
FatI CATG 1 cut(s) 459
Fnu4HI GCNGC 4 cut(s) 207, 321, 360, 486
Fsp4HI GCNGC 4 cut(s) 207, 321, 360, 486
FspBI CTAG 5 cut(s) 80, 107, 111, 123, 435
GlaI GCGC 3 cut(s) 358, 418, 420
GluI GCNGC 4 cut(s) 207, 321, 360, 486
HaeII RGCGCY 1 cut(s) 360
HaeIII GGCC 2 cut(s) 191, 439
HapII CCGG 2 cut(s) 294, 383
HhaI GCGC 3 cut(s) 359, 419, 421
Hin1II CATG 1 cut(s) 463
Hin6I GCGC 3 cut(s) 357, 417, 419
HinP1I GCGC 3 cut(s) 357, 417, 419
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HinfI GANTC 1 cut(s) 394
HpaI GTTAAC 1 cut(s) 517
HpaII CCGG 2 cut(s) 294, 383
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 1 cut(s) 399
Hpy188III TCNNGA 2 cut(s) 367, 403
Hpy8I GTNNAC 1 cut(s) 517
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 4 cut(s) 149, 279, 293, 450
HpyCH4III ACNGT 1 cut(s) 225
HpyCH4IV ACGT 2 cut(s) 236, 519
HpyCH4V TGCA 4 cut(s) 35, 351, 378, 479
HpyF10VI GCNNNNNNNGC 3 cut(s) 249, 485, 491
HpyF3I CTNAG 2 cut(s) 186, 429
HpySE526I ACGT 2 cut(s) 236, 519
Hsp92II CATG 1 cut(s) 463
HspAI GCGC 3 cut(s) 357, 417, 419
KspAI GTTAAC 1 cut(s) 517
Kzo9I GATC 4 cut(s) 93, 253, 399, 412
LguI GCTCTTC 1 cut(s) 158
LmnI GCTCC 2 cut(s) 141, 317
Lsp1109I GCAGC 2 cut(s) 193, 332
LweI GCATC 2 cut(s) 44, 442
MaeI CTAG 5 cut(s) 80, 107, 111, 123, 435
MaeII ACGT 2 cut(s) 236, 519
MaeIII GTNAC 1 cut(s) 52
MalI GATC 4 cut(s) 95, 255, 401, 414
MboI GATC 4 cut(s) 93, 253, 399, 412
MboII GAAGA 6 cut(s) 122, 145, 148, 166, 417, 492
MluCI AATT 2 cut(s) 30, 524
MnlI CCTC 6 cut(s) 18, 109, 138, 193, 339, 438
MseI TTAA 1 cut(s) 516
MspA1I CMGCKG 2 cut(s) 206, 362
MspI CCGG 2 cut(s) 294, 383
MspR9I CCNGG 1 cut(s) 384
Mva1269I GAATGC 1 cut(s) 378
MvnI CGCG 2 cut(s) 417, 419
MwoI GCNNNNNNNGC 3 cut(s) 249, 485, 491
NciI CCSGG 1 cut(s) 384
NcoI CCATGG 1 cut(s) 459
NdeII GATC 4 cut(s) 93, 253, 399, 412
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 1 cut(s) 463
NlaIV GGNNCC 3 cut(s) 137, 181, 202
PauI GCGCGC 1 cut(s) 417
PceI AGGCCT 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 158
PctI GAATGC 1 cut(s) 378
PfeI GAWTC 1 cut(s) 394
PkrI GCNGC 4 cut(s) 208, 322, 361, 487
Psp1406I AACGTT 1 cut(s) 519
PspN4I GGNNCC 3 cut(s) 137, 181, 202
PspPI GGNCC 1 cut(s) 179
PstNI CAGNNNCTG 1 cut(s) 340
PteI GCGCGC 1 cut(s) 417
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 4 cut(s) 207, 321, 360, 486
Sau3AI GATC 4 cut(s) 93, 253, 399, 412
Sau96I GGNCC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 384
SetI ASST 8 cut(s) 81, 85, 112, 239, 282, 350, 522, 537
SfaNI GCATC 2 cut(s) 44, 442
SfcI CTRYAG 1 cut(s) 214
SinI GGWCC 1 cut(s) 179
Sse9I AATT 2 cut(s) 30, 524
SseBI AGGCCT 1 cut(s) 439
SsiI CCGC 3 cut(s) 204, 360, 486
SspMI CTAG 5 cut(s) 80, 107, 111, 123, 435
StuI AGGCCT 1 cut(s) 439
StyD4I CCNGG 1 cut(s) 382
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 1 cut(s) 225
TaiI ACGT 2 cut(s) 239, 522
TaqI TCGA 2 cut(s) 92, 443
TaqII GACCGA 1 cut(s) 167
TasI AATT 2 cut(s) 30, 524
TauI GCSGC 2 cut(s) 362, 488
TfiI GAWTC 1 cut(s) 394
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TseI GCWGC 2 cut(s) 206, 320
TspDTI ATGAA 2 cut(s) 17, 498
TspGWI ACGGA 2 cut(s) 246, 330
VpaK11BI GGWCC 1 cut(s) 179
XagI CCTNNNNNAGG 1 cut(s) 328
XapI RAATTY 1 cut(s) 524
XspI CTAG 5 cut(s) 80, 107, 111, 123, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.