FvH4_2g31511

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008272
Physical Location & Seq
Forward (+)
24129771 .. 24133319
3549 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31511.t5

Sequence Viewer

Length: 1623 bp
ATGGCTTCCAGTATGACAGAGGGTTCAGCAGGACAACAAGAACAACCTGCCCCAAGATTGCAGATTTACTCCACCTCTGATACAGTCTCTCCCTTCTGGAGAGAGAAGTATGAAAGAGAAGCTAAGAAGTACTGGGATGTCTTTTATAAGCGACATCAAGATAGGTTTTTCAAAGATCGGCACTACTTGGATAAGGAATGGGGCCAATACTTCTCTGGAGCTGGGCAGAAATTTATTTTAGAGGTTGGCTGTGGAGCTGGAAACACTATCTTTCCACTGGTTGCGACATACACAGATGTTTTTGTCCATGCTTGTGATTTTTCACCAAATGCTGTGAACTTGGTTAAGAAACATAAAGACTTTACAGAATCCCGAGTTAACGCATTCGTCTGTGATTTGACTATTGACGATCTGAGCCAGCAGATATCTCCATCTTCAGTAGATATTGTAACCATGATTTTTGTGTTATCTGCAGTATCTCCTGAGAAGATGTCTCTCGTAGTGCAGAATATAAGAAAAATTCTCAAGCCAAGTGGTCGTGTGCTATTTCGCGATTATGCTACTGGTGACCTCGCTCAGGAAAGATTAAACTGCAAGGACCAAAAGATTAGTGAAAACTTTTATGCCAGAGGCGATGGCACTAGGGCTTTCTACTTCTCTAATGAGTTCTTGACAAGCTTATTCAAAGAAAATGGTTTTGATGTTGAGGAACTTGATTTGTGCTGCAAACAAGTTGAGAACCGGTCGCGGGAGTTGGTGATGAATAGACGGTGGGTTCAAGCTGTATTCTGCCTTTCAGATAATACTAACTCTAATACCAACTCAGAAACTGCAATCAGGATAGACCATCCTAGTGGTCAAGGGATTGAAGGGAAAATGGAGCTGGATGTCAACGATAACATCTTGCCAAAACCTGTAAATGATTTGGAGGTGATTGAACTCAAGCTGGGAGGTTGGACTTTCAAAATCAAAGTGCTATCGAAAGAGAACCAACACACCTGCAAATCAACTGGTTTAATGTTATGGGAATCAGCTCGGTTTATGGCTCCTGTTATAGCAAGAAATCCAACTATTGTTGCTGGAAAACGGGTGTTGGAGTTGGGATCTGGCTGTGCAGGCATTTGTTCTATGATTGCTGCTAGATCTGCGGAACTTGTATTGGCCACTGATGGAGACACCAAAGCACTCGACCTTCTGGTGGAAAATGTCATGTCAAATCTTAGACCGCCAATTTCAGACAAATTTACTACAAGAATATTAGAATGGGGAAATAAAGACCATATAGAAGCCATCAAGCAAGTTAACAGTGGAGGATTTGATATCATATTAGGTACAGATGTCACTTACATACCTGATGCTATTTTGCCCTTCTTTCAAACTGCAAAGGAGTTGATTTCATCTAATGATCAAGCAGCCCTAATCCTCTGTCATATTTTTCGTCGGGTAGATGAGCCTTCAATACTTTCAGCAGCTTCTCGATTTGGTTTTAAGCTAGTTGATAAGTGGCCTGTAGAAACTCCTATTACTCCATCACAAAGTATCATCAATTCTTGGTTCCCTGAAAACGGCTCTGTGGAAGTATCAAGGGGGCCATTACATATTTTGTACTTCCAGAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_16 Methyltransf_23 Methyltransf_25

Protein Analysis

541

Amino Acids

60.81

Weight (kDa)

5.75

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 76 - 237 5.4e-12 Methyltransferase domain
Methyltransf_25 PF13649 79 - 179 4.6e-13 Methyltransferase domain
Methyltransf_31 PF13847 79 - 196 8.9e-11 Methyltransferase domain
Methyltransf_12 PF08242 80 - 181 2.6e-16 Methyltransferase domain
Methyltransf_11 PF08241 80 - 183 2.9e-10 Methyltransferase domain
Methyltransf_16 PF10294 353 - 507 7.3e-15 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 147
AarI CACCTGC 1 cut(s) 1007
Acc36I ACCTGC 2 cut(s) 55, 1007
AccII CGCG 2 cut(s) 552, 748
AciI CCGC 3 cut(s) 748, 1148, 1226
AclWI GGATC 1 cut(s) 1111
AcoI YGGCCR 1 cut(s) 1161
AcsI RAATTY 3 cut(s) 230, 519, 1241
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 3 cut(s) 131, 1333, 1607
AfiI CCNNNNNNNGG 4 cut(s) 577, 748, 1198, 1565
AgeI ACCGGT 1 cut(s) 741
AgsI TTSAA 8 cut(s) 172, 685, 779, 869, 938, 964, 1376, 1458
Alw26I GTCTC 3 cut(s) 91, 498, 1167
AlwI GGATC 1 cut(s) 1111
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 1 cut(s) 372
AoxI GGCC 4 cut(s) 202, 1161, 1505, 1589
ApeKI GCWGC 4 cut(s) 723, 1136, 1412, 1469
ApoI RAATTY 3 cut(s) 230, 519, 1241
AsiGI ACCGGT 1 cut(s) 741
AspS9I GGNCC 3 cut(s) 202, 598, 1589
AsuHPI GGTGA 4 cut(s) 315, 578, 769, 943
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 1 cut(s) 598
BaeI ACNNNNGTAYC 2 cut(s) 1323, 1356
BalI TGGCCA 1 cut(s) 1163
BbvI GCAGC 4 cut(s) 710, 1123, 1424, 1481
BccI CCATC 6 cut(s) 439, 629, 855, 1163, 1298, 1537
BceAI ACGGC 1 cut(s) 1582
BclI TGATCA 1 cut(s) 1405
BcoDI GTCTC 3 cut(s) 91, 498, 1167
BfaI CTAG 4 cut(s) 642, 852, 1140, 1493
BfmI CTRYAG 2 cut(s) 471, 1509
BfuAI ACCTGC 2 cut(s) 55, 1007
BglII AGATCT 1 cut(s) 1142
BisI GCNGC 4 cut(s) 724, 1137, 1413, 1470
BlsI GCNGC 4 cut(s) 725, 1138, 1414, 1471
BmcAI AGTACT 1 cut(s) 131
Bme18I GGWCC 1 cut(s) 598
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 3 cut(s) 202, 598, 1589
BmiI GGNNCC 4 cut(s) 203, 1047, 1556, 1590
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 1345
BmuI ACTGGG 1 cut(s) 142
BpmI CTGGAG 2 cut(s) 118, 237
Bpu10I CCTNAGC 1 cut(s) 576
BpuEI CTTGAG 2 cut(s) 509, 926
BsaWI WCCGGW 1 cut(s) 741
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 4 cut(s) 577, 748, 1198, 1565
Bse118I RCCGGY 1 cut(s) 741
Bse1I ACTGG 5 cut(s) 9, 137, 282, 568, 1015
BseGI GGATG 3 cut(s) 142, 847, 892
BseLI CCNNNNNNNGG 4 cut(s) 577, 748, 1198, 1565
BseMII CTCAG 4 cut(s) 404, 474, 590, 837
BseNI ACTGG 5 cut(s) 9, 137, 282, 568, 1015
BseXI GCAGC 4 cut(s) 710, 1123, 1424, 1481
BseYI CCCAGC 2 cut(s) 221, 946
BsgI GTGCAG 2 cut(s) 524, 1134
Bsh1236I CGCG 2 cut(s) 552, 748
Bsh1285I CGRYCG 1 cut(s) 746
BshFI GGCC 4 cut(s) 204, 1163, 1507, 1591
BshTI ACCGGT 1 cut(s) 741
BsiEI CGRYCG 1 cut(s) 746
BsiHKCI CYCGRG 1 cut(s) 372
BsiSI CCGG 1 cut(s) 742
BslI CCNNNNNNNGG 4 cut(s) 577, 748, 1198, 1565
BsmAI GTCTC 3 cut(s) 91, 498, 1167
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 4 cut(s) 204, 1163, 1507, 1591
BsoBI CYCGRG 1 cut(s) 372
Bsp143I GATC 5 cut(s) 175, 409, 1103, 1142, 1405
Bsp68I TCGCGA 1 cut(s) 552
BspACI CCGC 3 cut(s) 748, 1148, 1226
BspANI GGCC 4 cut(s) 204, 1163, 1507, 1591
BspCNI CTCAG 4 cut(s) 405, 475, 589, 836
BspFNI CGCG 2 cut(s) 552, 748
BspLI GGNNCC 4 cut(s) 203, 1047, 1556, 1590
BspMAI CTGCAG 1 cut(s) 475
BspMI ACCTGC 2 cut(s) 55, 1007
BspPI GGATC 1 cut(s) 1111
BsrFI RCCGGY 1 cut(s) 741
BsrI ACTGG 5 cut(s) 9, 137, 282, 568, 1015
BssAI RCCGGY 1 cut(s) 741
BssMI GATC 5 cut(s) 175, 409, 1103, 1142, 1405
Bst4CI ACNGT 3 cut(s) 85, 771, 1307
BstC8I GCNNGC 2 cut(s) 419, 1117
BstDEI CTNAG 6 cut(s) 123, 413, 483, 576, 823, 1220
BstEII GGTNACC 1 cut(s) 566
BstENI CCTNNNNNAGG 1 cut(s) 575
BstF5I GGATG 3 cut(s) 142, 847, 892
BstFNI CGCG 2 cut(s) 552, 748
BstKTI GATC 5 cut(s) 178, 412, 1106, 1145, 1408
BstMAI GTCTC 3 cut(s) 91, 498, 1167
BstMBI GATC 5 cut(s) 175, 409, 1103, 1142, 1405
BstMCI CGRYCG 1 cut(s) 746
BstMWI GCNNNNNNNGC 2 cut(s) 1116, 1145
BstPI GGTNACC 1 cut(s) 566
BstSFI CTRYAG 2 cut(s) 471, 1509
BstUI CGCG 2 cut(s) 552, 748
BstV1I GCAGC 4 cut(s) 710, 1123, 1424, 1481
BstX2I RGATCY 2 cut(s) 1103, 1142
BstXI CCANNNNNNTGG 1 cut(s) 854
BstYI RGATCY 2 cut(s) 1103, 1142
BsuRI GGCC 4 cut(s) 204, 1163, 1507, 1591
BtgZI GCGATG 1 cut(s) 648
BtsCI GGATG 3 cut(s) 142, 847, 892
BtsIMutI CAGTG 3 cut(s) 275, 1164, 1312
BtuMI TCGCGA 1 cut(s) 552
BveI ACCTGC 2 cut(s) 55, 1007
Cac8I GCNNGC 2 cut(s) 419, 1117
CaiI CAGNNNCTG 1 cut(s) 830
Cfr10I RCCGGY 1 cut(s) 741
Cfr13I GGNCC 3 cut(s) 202, 598, 1589
Csp6I GTAC 3 cut(s) 130, 1332, 1606
CspAI ACCGGT 1 cut(s) 741
CspCI CAANNNNNGTGG 2 cut(s) 514, 549
CviAII CATG 3 cut(s) 308, 454, 1210
CviQI GTAC 3 cut(s) 130, 1332, 1606
DdeI CTNAG 6 cut(s) 123, 413, 483, 576, 823, 1220
DpnI GATC 5 cut(s) 177, 411, 1105, 1144, 1407
DpnII GATC 5 cut(s) 175, 409, 1103, 1142, 1405
EaeI YGGCCR 1 cut(s) 1161
Eco32I GATATC 2 cut(s) 426, 1321
Eco47I GGWCC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 372
Eco91I GGTNACC 1 cut(s) 566
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoO65I GGTNACC 1 cut(s) 566
EcoRV GATATC 2 cut(s) 426, 1321
FaeI CATG 3 cut(s) 311, 457, 1213
FatI CATG 3 cut(s) 307, 453, 1209
FauI CCCGC 1 cut(s) 741
FbaI TGATCA 1 cut(s) 1405
Fnu4HI GCNGC 4 cut(s) 724, 1137, 1413, 1470
FokI GGATG 3 cut(s) 149, 834, 899
Fsp4HI GCNGC 4 cut(s) 724, 1137, 1413, 1470
FspBI CTAG 4 cut(s) 642, 852, 1140, 1493
GluI GCNGC 4 cut(s) 724, 1137, 1413, 1470
GsaI CCCAGC 2 cut(s) 225, 950
GsuI CTGGAG 2 cut(s) 118, 237
HaeIII GGCC 4 cut(s) 204, 1163, 1507, 1591
HapII CCGG 1 cut(s) 742
Hin1II CATG 3 cut(s) 311, 457, 1213
HincII GTYRAC 3 cut(s) 379, 892, 1303
HindII GTYRAC 3 cut(s) 379, 892, 1303
HindIII AAGCTT 1 cut(s) 676
HinfI GANTC 2 cut(s) 368, 1028
HpaI GTTAAC 2 cut(s) 379, 1303
HpaII CCGG 1 cut(s) 742
HphI GGTGA 4 cut(s) 315, 578, 769, 943
Hpy166II GTNNAC 4 cut(s) 337, 379, 892, 1303
Hpy188I TCNGA 5 cut(s) 79, 414, 799, 826, 1237
Hpy8I GTNNAC 4 cut(s) 337, 379, 892, 1303
Hpy99I CGWCG 1 cut(s) 1443
HpyAV CCTTC 6 cut(s) 103, 863, 1202, 1378, 1464, 1609
HpyCH4III ACNGT 3 cut(s) 85, 771, 1307
HpyCH4V TGCA 9 cut(s) 61, 473, 505, 594, 726, 833, 1002, 1115, 1382
HpyF10VI GCNNNNNNNGC 2 cut(s) 1116, 1145
HpyF3I CTNAG 6 cut(s) 123, 413, 483, 576, 823, 1220
Hsp92II CATG 3 cut(s) 311, 457, 1213
Ksp22I TGATCA 1 cut(s) 1405
KspAI GTTAAC 2 cut(s) 379, 1303
Kzo9I GATC 5 cut(s) 175, 409, 1103, 1142, 1405
LmnI GCTCC 4 cut(s) 218, 254, 880, 1051
Lsp1109I GCAGC 4 cut(s) 710, 1123, 1424, 1481
LweI GCATC 1 cut(s) 1345
MaeI CTAG 4 cut(s) 642, 852, 1140, 1493
MaeIII GTNAC 3 cut(s) 448, 566, 1339
MalI GATC 5 cut(s) 177, 411, 1105, 1144, 1407
MboI GATC 5 cut(s) 175, 409, 1103, 1142, 1405
MboII GAAGA 2 cut(s) 426, 499
MflI RGATCY 2 cut(s) 1103, 1142
MlsI TGGCCA 1 cut(s) 1163
MluCI AATT 5 cut(s) 230, 519, 1230, 1241, 1546
MluNI TGGCCA 1 cut(s) 1163
MmeI TCCRAC 3 cut(s) 935, 1074, 1091
Mox20I TGGCCA 1 cut(s) 1163
MscI TGGCCA 1 cut(s) 1163
MseI TTAA 6 cut(s) 345, 378, 587, 1016, 1302, 1488
MslI CAYNNNNRTG 2 cut(s) 312, 852
Msp20I TGGCCA 1 cut(s) 1163
MspI CCGG 1 cut(s) 742
Mva1269I GAATGC 1 cut(s) 383
MvnI CGCG 2 cut(s) 552, 748
MwoI GCNNNNNNNGC 2 cut(s) 1116, 1145
NdeII GATC 5 cut(s) 175, 409, 1103, 1142, 1405
NlaIII CATG 3 cut(s) 311, 457, 1213
NlaIV GGNNCC 4 cut(s) 203, 1047, 1556, 1590
NmuCI GTSAC 2 cut(s) 566, 1339
NruI TCGCGA 1 cut(s) 552
PaqCI CACCTGC 1 cut(s) 1007
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 368, 1028
PinAI ACCGGT 1 cut(s) 741
PkrI GCNGC 4 cut(s) 725, 1138, 1414, 1471
PsiI TTATAA 1 cut(s) 147
PspEI GGTNACC 1 cut(s) 566
PspFI CCCAGC 2 cut(s) 221, 946
PspN4I GGNNCC 4 cut(s) 203, 1047, 1556, 1590
PspPI GGNCC 3 cut(s) 202, 598, 1589
PstI CTGCAG 1 cut(s) 475
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 2 cut(s) 1103, 1142
RruI TCGCGA 1 cut(s) 552
RsaI GTAC 3 cut(s) 131, 1333, 1607
RsaNI GTAC 3 cut(s) 130, 1332, 1606
RseI CAYNNNNRTG 2 cut(s) 312, 852
SaqAI TTAA 6 cut(s) 345, 378, 587, 1016, 1302, 1488
SatI GCNGC 4 cut(s) 724, 1137, 1413, 1470
Sau3AI GATC 5 cut(s) 175, 409, 1103, 1142, 1405
Sau96I GGNCC 3 cut(s) 202, 598, 1589
ScaI AGTACT 1 cut(s) 131
SfaNI GCATC 1 cut(s) 1345
SfcI CTRYAG 2 cut(s) 471, 1509
SinI GGWCC 1 cut(s) 598
SmiMI CAYNNNNRTG 2 cut(s) 312, 852
SmlI CTYRAG 2 cut(s) 524, 941
SmoI CTYRAG 2 cut(s) 524, 941
Sse9I AATT 5 cut(s) 230, 519, 1230, 1241, 1546
SsiI CCGC 3 cut(s) 748, 1148, 1226
SspI AATATT 1 cut(s) 1257
SspMI CTAG 4 cut(s) 642, 852, 1140, 1493
TaaI ACNGT 3 cut(s) 85, 771, 1307
TaqI TCGA 3 cut(s) 980, 1188, 1477
TasI AATT 5 cut(s) 230, 519, 1230, 1241, 1546
TatI WGTACW 2 cut(s) 129, 1605
TfiI GAWTC 2 cut(s) 368, 1028
Tru1I TTAA 6 cut(s) 345, 378, 587, 1016, 1302, 1488
Tru9I TTAA 6 cut(s) 345, 378, 587, 1016, 1302, 1488
TscAI CASTG 3 cut(s) 282, 1171, 1312
TseFI GTSAC 2 cut(s) 566, 1339
TseI GCWGC 4 cut(s) 723, 1136, 1412, 1469
Tsp45I GTSAC 2 cut(s) 566, 1339
TspDTI ATGAA 3 cut(s) 126, 776, 1386
TspRI CASTG 3 cut(s) 282, 1171, 1312
VpaK11BI GGWCC 1 cut(s) 598
XagI CCTNNNNNAGG 1 cut(s) 575
XapI RAATTY 3 cut(s) 230, 519, 1241
XcmI CCANNNNNNNNNTGG 1 cut(s) 212
XspI CTAG 4 cut(s) 642, 852, 1140, 1493
ZrmI AGTACT 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.