Rh6BG512300

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0008272
Physical Location & Seq
Reverse (-)
70784283 .. 70787521
3239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG512300.1

Sequence Viewer

Length: 1494 bp
ATGGTTATTTTCAACTATCAGGGAGCTGGGCAGAAAGTTGTTTTAGAGGTTGGCTGTGGAGCTGGAAACACTATTTTTCCACTGGTTGCGATATATGCAGACATTTTTGTCCATGCTTGTGATTTTTCACCAAATGCTGTTAACTTGGTTAAGAAACATAAAGATTTTACAGAGTCCAGAGTTAATGCGTTCGTTTGTGATTTGACCATTGACGATCTGAGCAAGCAGATATCTCCATCTTCAGTAGATATTGTAACCATGATTTTTGTGTTATCTGCAGTATCTCCGGAGAAGATGTCTCTGGTAGTGCAGAATATTAGAAAGATTCTCAAGCCAAATGGTCGTGTGCTATTTCGTGATTATGCTACTGGTGACCTTGCTCAGGAAAGATTAAATTGCAAGGACCAAAAGATTAGTGAAAACTTTTATGCCAGGGGTGATGGCACTAGGGCTTTCTACTTCTCTAATGAGTTCTTGACAAGCTTATTTAAAGAAAACAGTTTTGATGTTGAGGAACTTGATCTGTGCTGCAAACAAGTTGAGAACCGGTCGCGGGAGTTGGTGATGAATAGGCGGTGGGTTCAAGCTGTATTCTGCCTTTCAGATGGTACGAACTCCACCACCAACTCAGAAGCTGCAATCAGAATAGACCATCCTGGTCAAGGGAAAATGGAGCTGGATGTTGACAATAACATCTTGAAGAAACCTGTAAACGATTCGGAGGTTGACATGTCCGACGGTGTGGCAGCAGAAATGTTTGGTCTTCCAACTTCGACCGATGGTGATAATGAGGTCATTGAGCTCGAGCTGGGAGGTTGGACTTTCAAAATCAAAGTGCTATCCAAAGAGTACCAACACACCTGCAAATCAACTGGTTTAATGTTATGGGAATCAGCTCGGTTTATGGCTCCTGTTTTAGCAAGAAATCCAGCAATTTTTGCTGGGAAACGGGTGTTGGAGTTGGGGTCTGGCTGTGCAGGCATTTGCTCTATGATTGCTGCTGGATCTGCAGACCTTGTGTTCGCCACTGATGGAGACACAAAAGCACTCGACCTACTGACAGAAAATGTCATGTCAAATCTTAGACCGCCAATTTCGGACAAATTTACTACAAGAATATTAGAGTGGGGAAATAAAGACCATATAGAAGCCATCAAGCAAGTTAACAGTGGAGGATTTGATATCATATTAGGCACAGATGTCACTTACATACCTGAAGCTATTTTGCCCTTGTTTCAAACGGCAAAGGAGTTGATTTCATCTAATAACAAGGATCACCAAGCAGCCCTAATCCTGTGTCATATTTTTCGTCGAGTAGATGAACCTTCCATACTTTCAGCAGCTTCTCGATTTGGTTTCAAGCTAGTCGATAAGTGGCCTGTAGAAATTCTTATTAATCCATCACAAAGCATCATCAAGTCTTGGTTCCTTGAAAACGGTTCAGCGGATGTTTCAAGGGGGCCGCTAAATATTTTGTACTTCCACGAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_16 Methyltransf_23 Methyltransf_25

Protein Analysis

497

Amino Acids

55.09

Weight (kDa)

5.14

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 12 - 172 5.8e-11 Methyltransferase domain
Methyltransf_31 PF13847 12 - 129 2.2e-11 Methyltransferase domain
Methyltransf_25 PF13649 14 - 114 4.3e-13 Methyltransferase domain
Methyltransf_12 PF08242 15 - 116 1.5e-16 Methyltransferase domain
Methyltransf_11 PF08241 15 - 118 1.1e-10 Methyltransferase domain
Methyltransf_16 PF10294 285 - 441 3.8e-15 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 869
AasI GACNNNNNNGTC 1 cut(s) 107
Acc36I ACCTGC 1 cut(s) 869
AccII CGCG 1 cut(s) 553
AccIII TCCGGA 1 cut(s) 286
AciI CCGC 5 cut(s) 553, 574, 1088, 1445, 1463
AclWI GGATC 2 cut(s) 1012, 1281
AcsI RAATTY 2 cut(s) 1103, 1386
AcuI CTGAAG 2 cut(s) 225, 1236
AfaI GTAC 3 cut(s) 610, 851, 1478
AfiI CCNNNNNNNGG 3 cut(s) 382, 553, 662
AflIII ACRYGT 1 cut(s) 729
AgeI ACCGGT 1 cut(s) 546
AgsI TTSAA 8 cut(s) 13, 584, 700, 826, 1238, 1360, 1433, 1455
AjnI CCWGG 2 cut(s) 431, 655
AjuI GAANNNNNNNTTGG 2 cut(s) 938, 970
Alw21I GWGCWC 1 cut(s) 804
Alw26I GTCTC 2 cut(s) 303, 1029
AlwI GGATC 2 cut(s) 1012, 1281
AlwNI CAGNNNCTG 1 cut(s) 635
Ama87I CYCGRG 1 cut(s) 803
Aor13HI TCCGGA 1 cut(s) 286
AoxI GGCC 2 cut(s) 1376, 1460
ApeKI GCWGC 6 cut(s) 528, 635, 746, 998, 1283, 1340
ApoI RAATTY 2 cut(s) 1103, 1386
ArsI GACNNNNNNTTYG 2 cut(s) 1004, 1036
AseI ATTAAT 1 cut(s) 1395
AsiGI ACCGGT 1 cut(s) 546
AspS9I GGNCC 2 cut(s) 403, 1460
AsuHPI GGTGA 6 cut(s) 120, 383, 449, 574, 794, 1268
AvaI CYCGRG 1 cut(s) 803
AvaII GGWCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 804
BauI CACGAG 1 cut(s) 1484
BbsI GAAGAC 1 cut(s) 755
Bbv12I GWGCWC 1 cut(s) 804
BbvI GCAGC 6 cut(s) 515, 622, 758, 985, 1295, 1352
BccI CCATC 8 cut(s) 244, 434, 599, 660, 773, 1025, 1160, 1408
BceAI ACGGC 1 cut(s) 1257
BciT130I CCWGG 2 cut(s) 433, 657
BcoDI GTCTC 2 cut(s) 303, 1029
BfaI CTAG 2 cut(s) 447, 1364
BfmI CTRYAG 3 cut(s) 276, 1008, 1380
BfuAI ACCTGC 1 cut(s) 869
BisI GCNGC 7 cut(s) 529, 636, 747, 999, 1284, 1341, 1463
BlsI GCNGC 7 cut(s) 530, 637, 748, 1000, 1285, 1342, 1464
Bme1390I CCNGG 2 cut(s) 433, 657
Bme18I GGWCC 1 cut(s) 403
BmeT110I CYCGRG 1 cut(s) 803
BmgT120I GGNCC 2 cut(s) 403, 1460
BmiI GGNNCC 3 cut(s) 909, 1427, 1461
BmrFI CCNGG 2 cut(s) 433, 657
BmsI GCATC 1 cut(s) 1419
BpiI GAAGAC 1 cut(s) 755
Bpu10I CCTNAGC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 432
BsaWI WCCGGW 2 cut(s) 286, 546
BsaXI ACNNNNNCTCC 4 cut(s) 281, 311, 950, 980
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 553, 662
Bse118I RCCGGY 1 cut(s) 546
Bse1I ACTGG 3 cut(s) 87, 373, 877
BseAI TCCGGA 1 cut(s) 286
BseBI CCWGG 2 cut(s) 433, 657
BseDI CCNNGG 1 cut(s) 432
BseGI GGATG 3 cut(s) 652, 685, 1453
BseLI CCNNNNNNNGG 3 cut(s) 382, 553, 662
BseMII CTCAG 3 cut(s) 209, 395, 642
BseNI ACTGG 3 cut(s) 87, 373, 877
BseXI GCAGC 6 cut(s) 515, 622, 758, 985, 1295, 1352
BseYI CCCAGC 3 cut(s) 26, 808, 941
BsgI GTGCAG 2 cut(s) 329, 996
Bsh1236I CGCG 1 cut(s) 553
Bsh1285I CGRYCG 2 cut(s) 551, 777
BshFI GGCC 2 cut(s) 1378, 1462
BshTI ACCGGT 1 cut(s) 546
BsiEI CGRYCG 2 cut(s) 551, 777
BsiHKAI GWGCWC 1 cut(s) 804
BsiHKCI CYCGRG 1 cut(s) 803
BsiSI CCGG 2 cut(s) 287, 547
BslI CCNNNNNNNGG 3 cut(s) 382, 553, 662
BsmAI GTCTC 2 cut(s) 303, 1029
BsnI GGCC 2 cut(s) 1378, 1462
BsoBI CYCGRG 1 cut(s) 803
Bsp1286I GDGCHC 1 cut(s) 804
Bsp13I TCCGGA 1 cut(s) 286
Bsp143I GATC 4 cut(s) 214, 520, 1004, 1273
BspACI CCGC 5 cut(s) 553, 574, 1088, 1445, 1463
BspANI GGCC 2 cut(s) 1378, 1462
BspCNI CTCAG 3 cut(s) 210, 394, 641
BspEI TCCGGA 1 cut(s) 286
BspFNI CGCG 1 cut(s) 553
BspLI GGNNCC 3 cut(s) 909, 1427, 1461
BspMAI CTGCAG 2 cut(s) 280, 1012
BspMI ACCTGC 1 cut(s) 869
BspPI GGATC 2 cut(s) 1012, 1281
BsrFI RCCGGY 1 cut(s) 546
BsrI ACTGG 3 cut(s) 87, 373, 877
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 1 cut(s) 432
BssMI GATC 4 cut(s) 214, 520, 1004, 1273
BssSI CACGAG 1 cut(s) 1484
Bst2BI CACGAG 1 cut(s) 1484
Bst2UI CCWGG 2 cut(s) 433, 657
Bst4CI ACNGT 4 cut(s) 500, 740, 1169, 1439
BstAPI GCANNNNNTGC 1 cut(s) 938
BstC8I GCNNGC 2 cut(s) 224, 979
BstDEI CTNAG 4 cut(s) 218, 381, 628, 1082
BstEII GGTNACC 1 cut(s) 371
BstENI CCTNNNNNAGG 2 cut(s) 380, 660
BstF5I GGATG 3 cut(s) 652, 685, 1453
BstFNI CGCG 1 cut(s) 553
BstKTI GATC 4 cut(s) 217, 523, 1007, 1276
BstMAI GTCTC 2 cut(s) 303, 1029
BstMBI GATC 4 cut(s) 214, 520, 1004, 1273
BstMCI CGRYCG 2 cut(s) 551, 777
BstMWI GCNNNNNNNGC 4 cut(s) 95, 938, 978, 1007
BstNI CCWGG 2 cut(s) 433, 657
BstNSI RCATGY 1 cut(s) 733
BstPI GGTNACC 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 431, 655
BstSFI CTRYAG 3 cut(s) 276, 1008, 1380
BstUI CGCG 1 cut(s) 553
BstV1I GCAGC 6 cut(s) 515, 622, 758, 985, 1295, 1352
BstV2I GAAGAC 1 cut(s) 755
BstX2I RGATCY 1 cut(s) 1004
BstYI RGATCY 1 cut(s) 1004
BsuRI GGCC 2 cut(s) 1378, 1462
BtsCI GGATG 3 cut(s) 652, 685, 1453
BtsIMutI CAGTG 3 cut(s) 80, 1026, 1174
BveI ACCTGC 1 cut(s) 869
Cac8I GCNNGC 2 cut(s) 224, 979
CaiI CAGNNNCTG 1 cut(s) 635
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 2 cut(s) 403, 1460
Csp6I GTAC 3 cut(s) 609, 850, 1477
CspAI ACCGGT 1 cut(s) 546
CviAII CATG 4 cut(s) 113, 259, 730, 1072
CviQI GTAC 3 cut(s) 609, 850, 1477
DdeI CTNAG 4 cut(s) 218, 381, 628, 1082
DpnI GATC 4 cut(s) 216, 522, 1006, 1275
DpnII GATC 4 cut(s) 214, 520, 1004, 1273
DraI TTTAAA 1 cut(s) 490
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
Ecl136II GAGCTC 1 cut(s) 802
Eco24I GRGCYC 1 cut(s) 804
Eco32I GATATC 2 cut(s) 231, 1183
Eco47I GGWCC 1 cut(s) 403
Eco53kI GAGCTC 1 cut(s) 802
Eco57I CTGAAG 2 cut(s) 225, 1236
Eco88I CYCGRG 1 cut(s) 803
Eco91I GGTNACC 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 802
EcoNI CCTNNNNNAGG 2 cut(s) 380, 660
EcoO65I GGTNACC 1 cut(s) 371
EcoRII CCWGG 2 cut(s) 431, 655
EcoRV GATATC 2 cut(s) 231, 1183
EcoT38I GRGCYC 1 cut(s) 804
FaeI CATG 4 cut(s) 116, 262, 733, 1075
FatI CATG 4 cut(s) 112, 258, 729, 1071
FauI CCCGC 1 cut(s) 546
Fnu4HI GCNGC 7 cut(s) 529, 636, 747, 999, 1284, 1341, 1463
FokI GGATG 3 cut(s) 639, 692, 1460
FriOI GRGCYC 1 cut(s) 804
Fsp4HI GCNGC 7 cut(s) 529, 636, 747, 999, 1284, 1341, 1463
FspBI CTAG 2 cut(s) 447, 1364
GluI GCNGC 7 cut(s) 529, 636, 747, 999, 1284, 1341, 1463
GsaI CCCAGC 3 cut(s) 30, 812, 945
HaeIII GGCC 2 cut(s) 1378, 1462
HapII CCGG 2 cut(s) 287, 547
Hin1II CATG 4 cut(s) 116, 262, 733, 1075
HincII GTYRAC 4 cut(s) 142, 685, 727, 1165
HindII GTYRAC 4 cut(s) 142, 685, 727, 1165
HindIII AAGCTT 1 cut(s) 481
HinfI GANTC 4 cut(s) 173, 325, 716, 890
HpaI GTTAAC 2 cut(s) 142, 1165
HpaII CCGG 2 cut(s) 287, 547
HphI GGTGA 6 cut(s) 120, 383, 449, 574, 794, 1268
Hpy166II GTNNAC 5 cut(s) 142, 685, 712, 727, 1165
Hpy188I TCNGA 7 cut(s) 219, 604, 631, 644, 721, 736, 1099
Hpy188III TCNNGA 7 cut(s) 177, 287, 356, 383, 475, 697, 1347
Hpy8I GTNNAC 5 cut(s) 142, 685, 712, 727, 1165
Hpy99I CGWCG 2 cut(s) 740, 1314
HpyAV CCTTC 1 cut(s) 1335
HpyCH4III ACNGT 4 cut(s) 500, 740, 1169, 1439
HpyCH4V TGCA 9 cut(s) 98, 278, 310, 399, 531, 638, 864, 977, 1010
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 938, 978, 1007
HpyF3I CTNAG 4 cut(s) 218, 381, 628, 1082
Hsp92II CATG 4 cut(s) 116, 262, 733, 1075
Kpn2I TCCGGA 1 cut(s) 286
KspAI GTTAAC 2 cut(s) 142, 1165
Kzo9I GATC 4 cut(s) 214, 520, 1004, 1273
LmnI GCTCC 4 cut(s) 23, 59, 673, 913
Lsp1109I GCAGC 6 cut(s) 515, 622, 758, 985, 1295, 1352
LweI GCATC 1 cut(s) 1419
MaeI CTAG 2 cut(s) 447, 1364
MaeIII GTNAC 3 cut(s) 253, 371, 1201
MalI GATC 4 cut(s) 216, 522, 1006, 1275
MboI GATC 4 cut(s) 214, 520, 1004, 1273
MboII GAAGA 4 cut(s) 231, 304, 712, 755
MflI RGATCY 1 cut(s) 1004
MhlI GDGCHC 1 cut(s) 804
MluCI AATT 5 cut(s) 394, 933, 1092, 1103, 1386
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 4 cut(s) 759, 791, 797, 936
MnlI CCTC 7 cut(s) 40, 505, 715, 784, 806, 1166, 1480
MroI TCCGGA 1 cut(s) 286
MseI TTAA 8 cut(s) 141, 150, 183, 392, 489, 878, 1164, 1395
MslI CAYNNNNRTG 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 1445
MspI CCGG 2 cut(s) 287, 547
MspR9I CCNGG 2 cut(s) 433, 657
MvaI CCWGG 2 cut(s) 433, 657
MvnI CGCG 1 cut(s) 553
MwoI GCNNNNNNNGC 4 cut(s) 95, 938, 978, 1007
NdeII GATC 4 cut(s) 214, 520, 1004, 1273
NlaIII CATG 4 cut(s) 116, 262, 733, 1075
NlaIV GGNNCC 3 cut(s) 909, 1427, 1461
NmuCI GTSAC 2 cut(s) 371, 1201
NspI RCATGY 1 cut(s) 733
PaeR7I CTCGAG 1 cut(s) 803
PaqCI CACCTGC 1 cut(s) 869
PciI ACATGT 1 cut(s) 729
PfeI GAWTC 3 cut(s) 325, 716, 890
PinAI ACCGGT 1 cut(s) 546
PkrI GCNGC 7 cut(s) 530, 637, 748, 1000, 1285, 1342, 1464
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PscI ACATGT 1 cut(s) 729
PshBI ATTAAT 1 cut(s) 1395
Psp124BI GAGCTC 1 cut(s) 804
Psp6I CCWGG 2 cut(s) 431, 655
PspEI GGTNACC 1 cut(s) 371
PspFI CCCAGC 3 cut(s) 26, 808, 941
PspGI CCWGG 2 cut(s) 431, 655
PspN4I GGNNCC 3 cut(s) 909, 1427, 1461
PspPI GGNCC 2 cut(s) 403, 1460
PspXI VCTCGAGB 1 cut(s) 803
PsrI GAACNNNNNNTAC 2 cut(s) 1314, 1346
PstI CTGCAG 2 cut(s) 280, 1012
PstNI CAGNNNCTG 1 cut(s) 635
PsuI RGATCY 1 cut(s) 1004
RsaI GTAC 3 cut(s) 610, 851, 1478
RsaNI GTAC 3 cut(s) 609, 850, 1477
RseI CAYNNNNRTG 1 cut(s) 117
SacI GAGCTC 1 cut(s) 804
SaqAI TTAA 8 cut(s) 141, 150, 183, 392, 489, 878, 1164, 1395
SatI GCNGC 7 cut(s) 529, 636, 747, 999, 1284, 1341, 1463
Sau3AI GATC 4 cut(s) 214, 520, 1004, 1273
Sau96I GGNCC 2 cut(s) 403, 1460
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 433, 657
SduI GDGCHC 1 cut(s) 804
SfaNI GCATC 1 cut(s) 1419
SfcI CTRYAG 3 cut(s) 276, 1008, 1380
Sfr274I CTCGAG 1 cut(s) 803
SinI GGWCC 1 cut(s) 403
SlaI CTCGAG 1 cut(s) 803
SmiMI CAYNNNNRTG 1 cut(s) 117
SmlI CTYRAG 2 cut(s) 329, 803
SmoI CTYRAG 2 cut(s) 329, 803
Sse9I AATT 5 cut(s) 394, 933, 1092, 1103, 1386
SsiI CCGC 5 cut(s) 553, 574, 1088, 1445, 1463
SspI AATATT 3 cut(s) 316, 1119, 1471
SspMI CTAG 2 cut(s) 447, 1364
SstI GAGCTC 1 cut(s) 804
StyD4I CCNGG 2 cut(s) 431, 655
TaaI ACNGT 4 cut(s) 500, 740, 1169, 1439
TaqI TCGA 6 cut(s) 773, 804, 1050, 1312, 1348, 1368
TaqII GACCGA 1 cut(s) 791
TasI AATT 5 cut(s) 394, 933, 1092, 1103, 1386
TatI WGTACW 1 cut(s) 1476
TauI GCSGC 1 cut(s) 1465
TfiI GAWTC 3 cut(s) 325, 716, 890
Tru1I TTAA 8 cut(s) 141, 150, 183, 392, 489, 878, 1164, 1395
Tru9I TTAA 8 cut(s) 141, 150, 183, 392, 489, 878, 1164, 1395
TscAI CASTG 3 cut(s) 87, 1033, 1174
TseFI GTSAC 2 cut(s) 371, 1201
TseI GCWGC 6 cut(s) 528, 635, 746, 998, 1283, 1340
Tsp45I GTSAC 2 cut(s) 371, 1201
TspDTI ATGAA 3 cut(s) 581, 1248, 1335
TspRI CASTG 3 cut(s) 87, 1033, 1174
VpaK11BI GGWCC 1 cut(s) 403
VspI ATTAAT 1 cut(s) 1395
XagI CCTNNNNNAGG 2 cut(s) 380, 660
XapI RAATTY 2 cut(s) 1103, 1386
XceI RCATGY 1 cut(s) 733
XhoI CTCGAG 1 cut(s) 803
XspI CTAG 2 cut(s) 447, 1364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.