Rh6AG501600

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0008272
Physical Location & Seq
Reverse (-)
67557891 .. 67560884
2994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG501600.1

Sequence Viewer

Length: 1689 bp
ATGTCTTCCAGTATGGAAGAAGCTTCAGCAGGACAACAAGAACAACCTACCCCAAGATTACAGATTTACACCACCTCTGATACTGTCTCTCCCTTCTGGAGAGACAAGTATGAAAGAGAGGCTAAGAAGTACTGGGATGTCTTTTATAAGCGACATCAAGATAGGTTTTTTAAAGATCGGCACTACTTGGACAAGGAATGGGGCCAATACTTTTCTGGAGCTGGGCAGAAAGTTGTTTTAGAGGTTGGCTGTGGAGCTGGAAACACTATTTTTCCACTGGTTGCGATATATGTAGACATTTTTGTCCATGCTTGTGATTTTTCACCAAATGCTGTTAACTTGGTTAAGAAACATAAAGATTTTACAGAGTCCAGAGTTAATGCATTCGTTTGTGATTTGACCATTGACGATCTGAGCAAGCAGATATCTCCATCTTCAGTAGATATTGTAACCATGATTTTTGTGTTATCTGCAGTATCTCCGGAGAAGATGTCTCTGGTAGTGCAGAATATTAGAAAGATTCTCAAGCCAAATGGTCGTGTGCTATTTCGCGATTATGCTACTGGTGACCTTGCTCAGGAAAGATTAAATTGCAAGGACCAAAAGATTAGTGAAAACTTTTATGCCAGGGGTGATGGCACTAGGGCTTTCTACTTCTCTAATGAGTTCTTGACAAGCTTATTTAAAGAAAATGGTTTTGATGTTGAGGAACTTGATCTGTGCTGCAAACAAGTTGAGAACCGGTCGCGGGAGTTGGTGATGAATAGGCGGTGGGTTCAAGCTGTATTCTGCCTTTCAGATGGTACAAACTCCACCACCAACTCAGAAGCTGCAATCAGAATAGACCATCCTGGTCAAGGGAAAATGGAGCTGGATGTTGACAATAACATCTTGAAGAAACCTGTAAACGATTTGGAGGTTGACATGTCCGACGGTGTGGCAACAGAAATGTTTGGTCTTCCAACTTCGACTGATGTTGATAATGAGGTCATTGAGCTCGAGCTGGGAGGTTGGACTTTCAAAATCAAAGTGCTATCCAAAGAGTACCAACACACCTGCAAATCAACTGGTTTAATGTTATGGGAATCAGCTCGGTTTATGGCTCCTGTTTTAGCAAGAAATCCAGCTATTTTTGCTGGGAAACGGGTGTTGGAGTTGGGGTCTGGCTGTGCAGGCATTTGCTCTATGATTGCTGCTGGATCTGCAGACCTTGTGTTCGCCACTGATGGAGACACAAAAGCACTCGACCTACTGACAGAAAATGTCATGTCAAATCTTAGACAGCCAATTTCGGACAAATTTACTACAAGAATATTAGAGTGGGGAAATAAAGACCATATAGAAGCCATCAAGCAAGTTAACAGTGGAGGATTTGATATCATATTAGGCACAGATGTCACTTACATACCTGAAGCTATTTTGCCCTTGTTTCAAACTGCAAAGGAGTTGATTTCATCTAACAACAAGGATCACCAAGCAGCCCTAATCCTGTGTCATATGTTTCGTCGAGTAGATGAACCTTCCATACTATCAGCAGCTTCTCGATTTGGTTTCAAGCTAGTCGATAAGTGGCCTGTAGAAATTCTTATTAATCCATCACAAAGCATCATCAATTCTTGGTTCCCTGAAAACGGCTCAGCGGATGTTTCAAGGGGGCCGTTAAATATTTTGTACTTCCACAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_16 Methyltransf_23 Methyltransf_25

Protein Analysis

562

Amino Acids

63.23

Weight (kDa)

5.36

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 71 - 237 3.6e-13 Methyltransferase domain
Methyltransf_31 PF13847 77 - 196 4.2e-11 Methyltransferase domain
Methyltransf_25 PF13649 79 - 179 9.3e-13 Methyltransferase domain
Methyltransf_12 PF08242 80 - 181 3.2e-16 Methyltransferase domain
Methyltransf_11 PF08241 80 - 183 1.4e-10 Methyltransferase domain
Methyltransf_16 PF10294 350 - 507 2.3e-15 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 147
AarI CACCTGC 1 cut(s) 1064
AasI GACNNNNNNGTC 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 1064
AccI GTMKAC 1 cut(s) 294
AccII CGCG 2 cut(s) 552, 748
AccIII TCCGGA 1 cut(s) 481
AciI CCGC 3 cut(s) 748, 769, 1640
AclWI GGATC 2 cut(s) 1207, 1476
AcsI RAATTY 2 cut(s) 1298, 1581
AcuI CTGAAG 3 cut(s) 9, 420, 1431
AfaI GTAC 4 cut(s) 131, 805, 1046, 1673
AfiI CCNNNNNNNGG 4 cut(s) 577, 748, 857, 1631
AflIII ACRYGT 1 cut(s) 924
AgeI ACCGGT 1 cut(s) 741
AgsI TTSAA 6 cut(s) 779, 895, 1021, 1433, 1555, 1650
AjnI CCWGG 2 cut(s) 626, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 1133, 1165
Alw21I GWGCWC 1 cut(s) 999
Alw26I GTCTC 4 cut(s) 91, 96, 498, 1224
AlwI GGATC 2 cut(s) 1207, 1476
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 1 cut(s) 998
Aor13HI TCCGGA 1 cut(s) 481
AoxI GGCC 3 cut(s) 202, 1571, 1655
ApeKI GCWGC 5 cut(s) 723, 830, 1193, 1478, 1535
ApoI RAATTY 2 cut(s) 1298, 1581
ArsI GACNNNNNNTTYG 2 cut(s) 1199, 1231
AseI ATTAAT 1 cut(s) 1590
AsiGI ACCGGT 1 cut(s) 741
AspS9I GGNCC 3 cut(s) 202, 598, 1655
AsuHPI GGTGA 5 cut(s) 315, 578, 644, 769, 1463
AvaI CYCGRG 1 cut(s) 998
AvaII GGWCC 1 cut(s) 598
BanII GRGCYC 1 cut(s) 999
BbsI GAAGAC 1 cut(s) 950
Bbv12I GWGCWC 1 cut(s) 999
BbvI GCAGC 5 cut(s) 710, 817, 1180, 1490, 1547
BccI CCATC 7 cut(s) 439, 629, 794, 855, 1220, 1355, 1603
BceAI ACGGC 2 cut(s) 1642, 1648
BciT130I CCWGG 2 cut(s) 628, 852
BcoDI GTCTC 4 cut(s) 91, 96, 498, 1224
BfaI CTAG 2 cut(s) 642, 1559
BfmI CTRYAG 3 cut(s) 471, 1203, 1575
BfuAI ACCTGC 1 cut(s) 1064
BisI GCNGC 5 cut(s) 724, 831, 1194, 1479, 1536
BlpI GCTNAGC 1 cut(s) 1636
BlsI GCNGC 5 cut(s) 725, 832, 1195, 1480, 1537
BmcAI AGTACT 1 cut(s) 131
Bme1390I CCNGG 2 cut(s) 628, 852
Bme18I GGWCC 1 cut(s) 598
BmeT110I CYCGRG 1 cut(s) 998
BmgT120I GGNCC 3 cut(s) 202, 598, 1655
BmiI GGNNCC 4 cut(s) 203, 1104, 1622, 1656
BmrFI CCNGG 2 cut(s) 628, 852
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 1614
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 950
BpmI CTGGAG 2 cut(s) 118, 237
Bpu10I CCTNAGC 1 cut(s) 576
Bpu1102I GCTNAGC 1 cut(s) 1636
BpuEI CTTGAG 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 627
BsaWI WCCGGW 2 cut(s) 481, 741
BsaXI ACNNNNNCTCC 6 cut(s) 73, 103, 476, 506, 1145, 1175
Bsc4I CCNNNNNNNGG 4 cut(s) 577, 748, 857, 1631
Bse118I RCCGGY 1 cut(s) 741
Bse1I ACTGG 5 cut(s) 9, 137, 282, 568, 1072
BseAI TCCGGA 1 cut(s) 481
BseBI CCWGG 2 cut(s) 628, 852
BseDI CCNNGG 1 cut(s) 627
BseGI GGATG 4 cut(s) 142, 847, 880, 1648
BseLI CCNNNNNNNGG 4 cut(s) 577, 748, 857, 1631
BseMII CTCAG 4 cut(s) 404, 590, 837, 1650
BseNI ACTGG 5 cut(s) 9, 137, 282, 568, 1072
BseXI GCAGC 5 cut(s) 710, 817, 1180, 1490, 1547
BseYI CCCAGC 3 cut(s) 221, 1003, 1136
BsgI GTGCAG 2 cut(s) 524, 1191
Bsh1236I CGCG 2 cut(s) 552, 748
Bsh1285I CGRYCG 1 cut(s) 746
BshFI GGCC 3 cut(s) 204, 1573, 1657
BshTI ACCGGT 1 cut(s) 741
BsiEI CGRYCG 1 cut(s) 746
BsiHKAI GWGCWC 1 cut(s) 999
BsiHKCI CYCGRG 1 cut(s) 998
BsiSI CCGG 2 cut(s) 482, 742
BslI CCNNNNNNNGG 4 cut(s) 577, 748, 857, 1631
BsmAI GTCTC 4 cut(s) 91, 96, 498, 1224
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 3 cut(s) 204, 1573, 1657
BsoBI CYCGRG 1 cut(s) 998
Bsp1286I GDGCHC 1 cut(s) 999
Bsp13I TCCGGA 1 cut(s) 481
Bsp143I GATC 5 cut(s) 175, 409, 715, 1199, 1468
Bsp1720I GCTNAGC 1 cut(s) 1636
Bsp68I TCGCGA 1 cut(s) 552
BspACI CCGC 3 cut(s) 748, 769, 1640
BspANI GGCC 3 cut(s) 204, 1573, 1657
BspCNI CTCAG 4 cut(s) 405, 589, 836, 1649
BspEI TCCGGA 1 cut(s) 481
BspFNI CGCG 2 cut(s) 552, 748
BspLI GGNNCC 4 cut(s) 203, 1104, 1622, 1656
BspMAI CTGCAG 2 cut(s) 475, 1207
BspMI ACCTGC 1 cut(s) 1064
BspPI GGATC 2 cut(s) 1207, 1476
BsrFI RCCGGY 1 cut(s) 741
BsrI ACTGG 5 cut(s) 9, 137, 282, 568, 1072
BssAI RCCGGY 1 cut(s) 741
BssECI CCNNGG 1 cut(s) 627
BssMI GATC 5 cut(s) 175, 409, 715, 1199, 1468
Bst2UI CCWGG 2 cut(s) 628, 852
Bst4CI ACNGT 3 cut(s) 85, 935, 1364
BstC8I GCNNGC 2 cut(s) 419, 1174
BstDEI CTNAG 6 cut(s) 123, 413, 576, 823, 1277, 1636
BstEII GGTNACC 1 cut(s) 566
BstENI CCTNNNNNAGG 2 cut(s) 575, 855
BstF5I GGATG 4 cut(s) 142, 847, 880, 1648
BstFNI CGCG 2 cut(s) 552, 748
BstKTI GATC 5 cut(s) 178, 412, 718, 1202, 1471
BstMAI GTCTC 4 cut(s) 91, 96, 498, 1224
BstMBI GATC 5 cut(s) 175, 409, 715, 1199, 1468
BstMCI CGRYCG 1 cut(s) 746
BstMWI GCNNNNNNNGC 3 cut(s) 1133, 1173, 1202
BstNI CCWGG 2 cut(s) 628, 852
BstNSI RCATGY 1 cut(s) 928
BstPI GGTNACC 1 cut(s) 566
BstSCI CCNGG 2 cut(s) 626, 850
BstSFI CTRYAG 3 cut(s) 471, 1203, 1575
BstUI CGCG 2 cut(s) 552, 748
BstV1I GCAGC 5 cut(s) 710, 817, 1180, 1490, 1547
BstV2I GAAGAC 1 cut(s) 950
BstX2I RGATCY 1 cut(s) 1199
BstYI RGATCY 1 cut(s) 1199
BsuRI GGCC 3 cut(s) 204, 1573, 1657
BtsCI GGATG 4 cut(s) 142, 847, 880, 1648
BtsIMutI CAGTG 3 cut(s) 275, 1221, 1369
BtuMI TCGCGA 1 cut(s) 552
BveI ACCTGC 1 cut(s) 1064
Cac8I GCNNGC 2 cut(s) 419, 1174
CaiI CAGNNNCTG 1 cut(s) 830
Cfr10I RCCGGY 1 cut(s) 741
Cfr13I GGNCC 3 cut(s) 202, 598, 1655
Csp6I GTAC 4 cut(s) 130, 804, 1045, 1672
CspAI ACCGGT 1 cut(s) 741
CviAII CATG 4 cut(s) 308, 454, 925, 1267
CviQI GTAC 4 cut(s) 130, 804, 1045, 1672
DdeI CTNAG 6 cut(s) 123, 413, 576, 823, 1277, 1636
DpnI GATC 5 cut(s) 177, 411, 717, 1201, 1470
DpnII GATC 5 cut(s) 175, 409, 715, 1199, 1468
DraI TTTAAA 2 cut(s) 172, 685
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
Ecl136II GAGCTC 1 cut(s) 997
Eco24I GRGCYC 1 cut(s) 999
Eco32I GATATC 2 cut(s) 426, 1378
Eco47I GGWCC 1 cut(s) 598
Eco53kI GAGCTC 1 cut(s) 997
Eco57I CTGAAG 3 cut(s) 9, 420, 1431
Eco88I CYCGRG 1 cut(s) 998
Eco91I GGTNACC 1 cut(s) 566
EcoICRI GAGCTC 1 cut(s) 997
EcoNI CCTNNNNNAGG 2 cut(s) 575, 855
EcoO65I GGTNACC 1 cut(s) 566
EcoRII CCWGG 2 cut(s) 626, 850
EcoRV GATATC 2 cut(s) 426, 1378
EcoT22I ATGCAT 1 cut(s) 385
EcoT38I GRGCYC 1 cut(s) 999
FaeI CATG 4 cut(s) 311, 457, 928, 1270
FatI CATG 4 cut(s) 307, 453, 924, 1266
FauI CCCGC 1 cut(s) 741
FauNDI CATATG 1 cut(s) 1497
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 5 cut(s) 724, 831, 1194, 1479, 1536
FokI GGATG 4 cut(s) 149, 834, 887, 1655
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 5 cut(s) 724, 831, 1194, 1479, 1536
FspBI CTAG 2 cut(s) 642, 1559
GluI GCNGC 5 cut(s) 724, 831, 1194, 1479, 1536
GsaI CCCAGC 3 cut(s) 225, 1007, 1140
GsuI CTGGAG 2 cut(s) 118, 237
HaeIII GGCC 3 cut(s) 204, 1573, 1657
HapII CCGG 2 cut(s) 482, 742
Hin1II CATG 4 cut(s) 311, 457, 928, 1270
HincII GTYRAC 4 cut(s) 337, 880, 922, 1360
HindII GTYRAC 4 cut(s) 337, 880, 922, 1360
HindIII AAGCTT 2 cut(s) 21, 676
HinfI GANTC 3 cut(s) 368, 520, 1085
HpaI GTTAAC 2 cut(s) 337, 1360
HpaII CCGG 2 cut(s) 482, 742
HphI GGTGA 5 cut(s) 315, 578, 644, 769, 1463
Hpy166II GTNNAC 6 cut(s) 295, 337, 880, 907, 922, 1360
Hpy188I TCNGA 7 cut(s) 79, 414, 799, 826, 839, 931, 1294
Hpy8I GTNNAC 6 cut(s) 295, 337, 880, 907, 922, 1360
Hpy99I CGWCG 2 cut(s) 935, 1509
HpyAV CCTTC 2 cut(s) 103, 1530
HpyCH4III ACNGT 3 cut(s) 85, 935, 1364
HpyF10VI GCNNNNNNNGC 3 cut(s) 1133, 1173, 1202
HpyF3I CTNAG 6 cut(s) 123, 413, 576, 823, 1277, 1636
Hsp92II CATG 4 cut(s) 311, 457, 928, 1270
Kpn2I TCCGGA 1 cut(s) 481
KspAI GTTAAC 2 cut(s) 337, 1360
Kzo9I GATC 5 cut(s) 175, 409, 715, 1199, 1468
LmnI GCTCC 4 cut(s) 218, 254, 868, 1108
Lsp1109I GCAGC 5 cut(s) 710, 817, 1180, 1490, 1547
LweI GCATC 1 cut(s) 1614
MaeI CTAG 2 cut(s) 642, 1559
MaeIII GTNAC 3 cut(s) 448, 566, 1396
MalI GATC 5 cut(s) 177, 411, 717, 1201, 1470
MboI GATC 5 cut(s) 175, 409, 715, 1199, 1468
MboII GAAGA 5 cut(s) 29, 426, 499, 907, 950
MflI RGATCY 1 cut(s) 1199
MhlI GDGCHC 1 cut(s) 999
MluCI AATT 5 cut(s) 589, 1287, 1298, 1581, 1612
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 4 cut(s) 954, 986, 992, 1131
MnlI CCTC 8 cut(s) 85, 112, 235, 700, 910, 979, 1001, 1361
Mph1103I ATGCAT 1 cut(s) 385
MroI TCCGGA 1 cut(s) 481
MslI CAYNNNNRTG 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 1640
MspI CCGG 2 cut(s) 482, 742
MspR9I CCNGG 2 cut(s) 628, 852
Mva1269I GAATGC 1 cut(s) 383
MvaI CCWGG 2 cut(s) 628, 852
MvnI CGCG 2 cut(s) 552, 748
MwoI GCNNNNNNNGC 3 cut(s) 1133, 1173, 1202
NdeI CATATG 1 cut(s) 1497
NdeII GATC 5 cut(s) 175, 409, 715, 1199, 1468
NlaIII CATG 4 cut(s) 311, 457, 928, 1270
NlaIV GGNNCC 4 cut(s) 203, 1104, 1622, 1656
NmuCI GTSAC 2 cut(s) 566, 1396
NruI TCGCGA 1 cut(s) 552
NsiI ATGCAT 1 cut(s) 385
NspI RCATGY 1 cut(s) 928
PaeR7I CTCGAG 1 cut(s) 998
PaqCI CACCTGC 1 cut(s) 1064
PciI ACATGT 1 cut(s) 924
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 520, 1085
PinAI ACCGGT 1 cut(s) 741
PkrI GCNGC 5 cut(s) 725, 832, 1195, 1480, 1537
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
PscI ACATGT 1 cut(s) 924
PshBI ATTAAT 1 cut(s) 1590
PsiI TTATAA 1 cut(s) 147
Psp124BI GAGCTC 1 cut(s) 999
Psp6I CCWGG 2 cut(s) 626, 850
PspEI GGTNACC 1 cut(s) 566
PspFI CCCAGC 3 cut(s) 221, 1003, 1136
PspGI CCWGG 2 cut(s) 626, 850
PspN4I GGNNCC 4 cut(s) 203, 1104, 1622, 1656
PspPI GGNCC 3 cut(s) 202, 598, 1655
PspXI VCTCGAGB 1 cut(s) 998
PsrI GAACNNNNNNTAC 2 cut(s) 1509, 1541
PstI CTGCAG 2 cut(s) 475, 1207
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 1 cut(s) 1199
RruI TCGCGA 1 cut(s) 552
RsaI GTAC 4 cut(s) 131, 805, 1046, 1673
RsaNI GTAC 4 cut(s) 130, 804, 1045, 1672
RseI CAYNNNNRTG 1 cut(s) 312
SacI GAGCTC 1 cut(s) 999
SatI GCNGC 5 cut(s) 724, 831, 1194, 1479, 1536
Sau3AI GATC 5 cut(s) 175, 409, 715, 1199, 1468
Sau96I GGNCC 3 cut(s) 202, 598, 1655
ScaI AGTACT 1 cut(s) 131
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 628, 852
SduI GDGCHC 1 cut(s) 999
SfaNI GCATC 1 cut(s) 1614
SfcI CTRYAG 3 cut(s) 471, 1203, 1575
Sfr274I CTCGAG 1 cut(s) 998
SinI GGWCC 1 cut(s) 598
SlaI CTCGAG 1 cut(s) 998
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 2 cut(s) 524, 998
SmoI CTYRAG 2 cut(s) 524, 998
Sse9I AATT 5 cut(s) 589, 1287, 1298, 1581, 1612
SsiI CCGC 3 cut(s) 748, 769, 1640
SspI AATATT 3 cut(s) 511, 1314, 1666
SspMI CTAG 2 cut(s) 642, 1559
SstI GAGCTC 1 cut(s) 999
StyD4I CCNGG 2 cut(s) 626, 850
TaaI ACNGT 3 cut(s) 85, 935, 1364
TaqI TCGA 6 cut(s) 968, 999, 1245, 1507, 1543, 1563
TasI AATT 5 cut(s) 589, 1287, 1298, 1581, 1612
TatI WGTACW 2 cut(s) 129, 1671
TfiI GAWTC 2 cut(s) 520, 1085
TscAI CASTG 3 cut(s) 282, 1228, 1369
TseFI GTSAC 2 cut(s) 566, 1396
TseI GCWGC 5 cut(s) 723, 830, 1193, 1478, 1535
Tsp45I GTSAC 2 cut(s) 566, 1396
TspDTI ATGAA 4 cut(s) 126, 776, 1443, 1530
TspRI CASTG 3 cut(s) 282, 1228, 1369
VpaK11BI GGWCC 1 cut(s) 598
VspI ATTAAT 1 cut(s) 1590
XagI CCTNNNNNAGG 2 cut(s) 575, 855
XapI RAATTY 2 cut(s) 1298, 1581
XceI RCATGY 1 cut(s) 928
XcmI CCANNNNNNNNNTGG 1 cut(s) 212
XhoI CTCGAG 1 cut(s) 998
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 2 cut(s) 642, 1559
ZrmI AGTACT 1 cut(s) 131
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.