Rw6G043680

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008272
Physical Location & Seq
Reverse (-)
66926707 .. 66929685
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043680.1

Sequence Viewer

Length: 1680 bp
ATGTCTTCCAGTATGGAAGAAGCTTCAGCAGGACAACAAGAACAACCTACCCCAAGATTACAGATTTACACCACCTCTGATACTGTCTCTCCCTTCTGGAGAGAGAAGTATGAAAGAGAGGCTAAGAAGTACTGGGATGTCTTTTATAAGCGACATCAAGATAGGTTTTTTAAAGATCGGCACTACTTGGACAAGGAATGGGGCCAATACTTTTCTGGAGCTGGGCAGAAAGTTGTTTTAGAGGTTGGCTGTGGAGCTGGAAACACTATTTTTCCACTGGTTGCTATATATGCAGACATTTTTGTCCATGCTTGTGATTTTTCACCAAATGCTGTTAACTTGGTCAAGAAACATAAAGATTTTACAGAGTCCAGAGTTAATGCATTCGTTTGTGATTTGACCATTGACGATCTGAGCAAGCAGATATCTCCATCTTCAGTAGATATTGTAACCATGATTTTTGTGTTATCTGCAGTATCTCCGGAGAAGATGTCTCTGGTAGTGCAGAATATTAGAAAGATTCTCAAGCCAAATGGTCGTGTGCTATTTCGCGATTATGCTACTGGTGACCTTGCTCAGGAAAGATTAAATTGCAAGGACCAAAAGATTAGTGAAAACTTTTATGCCAGGGGTGATGGCACTAGGGCTTTCTACTTCTCTAATGAGTTCTTGACAAGCTTATTTAAAGAAAATGGTTTTGATGTTGAGGAACTTGATCTGTGCTGCAAACAAGTTGAAAACCGGTCGCGGGAGTTGGTGATGAATAGGCGGTGGGTTCAAGCTGTATTCTGCCTTTCAGATGGTACAAACTCCACCACCAACTCAGAAGCTGCAATCAGAATAGACCATCCTGGAATGGAGCTGGATGTTGACAATAACATCTTGAAGAAACCTGTAAACGATTTGGAGGTTGACATGTCCGACGGTGTGGCAACAGAAATGTTTGGTCTTCCAACTTCGACTGATGGTGATAATGAGGTCATTGAGCTCGAGCTGGGAGGTTGGACTTTCAAAATCAAAGTGCTATCCAAAGAGTACCAACACACCTGCAAATCAACTGGTTTAATGTTATGGGAATCAGCTCGGTTTATGGCTCCTGTTTTAGCAAGAAATCCAGCTATTTTTTCTGGGAAACGGGTGTTGGAGTTGGGGTCTGGCTGTGCAGGCATTTGCTCTATGATTGCTGCTGGATCTGCAGACCTTGTGTTCGCCACTGATGGAGACACAAAAGCACTCGACCTACTGACAGAAAATGTCATGTCAAATCTTAGACCGCCAATTTCGGACAAATTTACTACAAGAATATTAGAGTGGGGAAATAAAGACCATATAGAAGCCATCAAGCAAGTTAACAGTGGAGGATTTGATATCATATTAGGCACGGATGTCACTTACATACCTGAAGCTATTTTGCCCTTGTTTCAAACTGCAAAGGAGTTGATTTCATCTAACAACAAGGATCACCAAGCAGCCCTAATCCTGTGTCATATTTTTCGTCGAGTAGATGAACCTTCCATACTATCAGCAGCTTCTCGATTTGGTTTTAAGCTAGTCGATAAGTGGCCTGTAGAAATTCTTATTAATCCATCACAAAGCATCATCAATTCTTGGTTCCCTGAAAACGGCTCAGCGGATGTTTCAAGGGGGCCGTTAAATATTTTGTACTTCCACAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_16 Methyltransf_23 Methyltransf_25

Protein Analysis

559

Amino Acids

62.83

Weight (kDa)

5.31

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 71 - 237 3.8e-13 Methyltransferase domain
Methyltransf_31 PF13847 77 - 196 2.3e-11 Methyltransferase domain
Methyltransf_25 PF13649 79 - 179 5.2e-13 Methyltransferase domain
Methyltransf_12 PF08242 80 - 181 1.8e-16 Methyltransferase domain
Methyltransf_11 PF08241 80 - 183 1.3e-10 Methyltransferase domain
Methyltransf_16 PF10294 347 - 503 2.4e-15 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 147
AarI CACCTGC 1 cut(s) 1055
AasI GACNNNNNNGTC 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 1055
AccII CGCG 2 cut(s) 552, 748
AccIII TCCGGA 1 cut(s) 481
AciI CCGC 4 cut(s) 748, 769, 1274, 1631
AclWI GGATC 2 cut(s) 1198, 1467
AcsI RAATTY 2 cut(s) 1289, 1572
AcuI CTGAAG 3 cut(s) 9, 420, 1422
AfaI GTAC 4 cut(s) 131, 805, 1037, 1664
AfiI CCNNNNNNNGG 3 cut(s) 577, 748, 1622
AflIII ACRYGT 1 cut(s) 915
AgeI ACCGGT 1 cut(s) 741
AgsI TTSAA 6 cut(s) 737, 779, 886, 1012, 1424, 1641
AjnI CCWGG 2 cut(s) 626, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 1124, 1156
Alw21I GWGCWC 1 cut(s) 990
Alw26I GTCTC 3 cut(s) 91, 498, 1215
AlwI GGATC 2 cut(s) 1198, 1467
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 1 cut(s) 989
Aor13HI TCCGGA 1 cut(s) 481
AoxI GGCC 3 cut(s) 202, 1562, 1646
ApeKI GCWGC 5 cut(s) 723, 830, 1184, 1469, 1526
ApoI RAATTY 2 cut(s) 1289, 1572
ArsI GACNNNNNNTTYG 2 cut(s) 1190, 1222
AseI ATTAAT 1 cut(s) 1581
AsiGI ACCGGT 1 cut(s) 741
AspS9I GGNCC 3 cut(s) 202, 598, 1646
AsuHPI GGTGA 6 cut(s) 315, 578, 644, 769, 980, 1454
AvaI CYCGRG 1 cut(s) 989
AvaII GGWCC 1 cut(s) 598
BanII GRGCYC 1 cut(s) 990
BbsI GAAGAC 1 cut(s) 941
Bbv12I GWGCWC 1 cut(s) 990
BbvI GCAGC 5 cut(s) 710, 817, 1171, 1481, 1538
BccI CCATC 8 cut(s) 439, 629, 794, 855, 959, 1211, 1346, 1594
BceAI ACGGC 2 cut(s) 1633, 1639
BciT130I CCWGG 2 cut(s) 628, 852
BcoDI GTCTC 3 cut(s) 91, 498, 1215
BfaI CTAG 2 cut(s) 642, 1550
BfmI CTRYAG 3 cut(s) 471, 1194, 1566
BfuAI ACCTGC 1 cut(s) 1055
BisI GCNGC 5 cut(s) 724, 831, 1185, 1470, 1527
BlpI GCTNAGC 1 cut(s) 1627
BlsI GCNGC 5 cut(s) 725, 832, 1186, 1471, 1528
BmcAI AGTACT 1 cut(s) 131
Bme1390I CCNGG 2 cut(s) 628, 852
Bme18I GGWCC 1 cut(s) 598
BmeT110I CYCGRG 1 cut(s) 989
BmgT120I GGNCC 3 cut(s) 202, 598, 1646
BmiI GGNNCC 4 cut(s) 203, 1095, 1613, 1647
BmrFI CCNGG 2 cut(s) 628, 852
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 1605
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 941
BpmI CTGGAG 2 cut(s) 118, 237
Bpu10I CCTNAGC 1 cut(s) 576
Bpu1102I GCTNAGC 1 cut(s) 1627
BpuEI CTTGAG 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 627
BsaWI WCCGGW 2 cut(s) 481, 741
BsaXI ACNNNNNCTCC 6 cut(s) 73, 103, 476, 506, 1136, 1166
Bsc4I CCNNNNNNNGG 3 cut(s) 577, 748, 1622
Bse118I RCCGGY 1 cut(s) 741
Bse1I ACTGG 5 cut(s) 9, 137, 282, 568, 1063
BseAI TCCGGA 1 cut(s) 481
BseBI CCWGG 2 cut(s) 628, 852
BseDI CCNNGG 1 cut(s) 627
BseGI GGATG 5 cut(s) 142, 847, 871, 1390, 1639
BseLI CCNNNNNNNGG 3 cut(s) 577, 748, 1622
BseMII CTCAG 4 cut(s) 404, 590, 837, 1641
BseNI ACTGG 5 cut(s) 9, 137, 282, 568, 1063
BseXI GCAGC 5 cut(s) 710, 817, 1171, 1481, 1538
BseYI CCCAGC 2 cut(s) 221, 994
BsgI GTGCAG 2 cut(s) 524, 1182
Bsh1236I CGCG 2 cut(s) 552, 748
Bsh1285I CGRYCG 1 cut(s) 746
BshFI GGCC 3 cut(s) 204, 1564, 1648
BshTI ACCGGT 1 cut(s) 741
BsiEI CGRYCG 1 cut(s) 746
BsiHKAI GWGCWC 1 cut(s) 990
BsiHKCI CYCGRG 1 cut(s) 989
BsiSI CCGG 2 cut(s) 482, 742
BslI CCNNNNNNNGG 3 cut(s) 577, 748, 1622
BsmAI GTCTC 3 cut(s) 91, 498, 1215
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 3 cut(s) 204, 1564, 1648
BsoBI CYCGRG 1 cut(s) 989
Bsp1286I GDGCHC 1 cut(s) 990
Bsp13I TCCGGA 1 cut(s) 481
Bsp143I GATC 5 cut(s) 175, 409, 715, 1190, 1459
Bsp1720I GCTNAGC 1 cut(s) 1627
Bsp68I TCGCGA 1 cut(s) 552
BspACI CCGC 4 cut(s) 748, 769, 1274, 1631
BspANI GGCC 3 cut(s) 204, 1564, 1648
BspCNI CTCAG 4 cut(s) 405, 589, 836, 1640
BspEI TCCGGA 1 cut(s) 481
BspFNI CGCG 2 cut(s) 552, 748
BspLI GGNNCC 4 cut(s) 203, 1095, 1613, 1647
BspMAI CTGCAG 2 cut(s) 475, 1198
BspMI ACCTGC 1 cut(s) 1055
BspPI GGATC 2 cut(s) 1198, 1467
BsrFI RCCGGY 1 cut(s) 741
BsrI ACTGG 5 cut(s) 9, 137, 282, 568, 1063
BssAI RCCGGY 1 cut(s) 741
BssECI CCNNGG 1 cut(s) 627
BssMI GATC 5 cut(s) 175, 409, 715, 1190, 1459
Bst2UI CCWGG 2 cut(s) 628, 852
Bst4CI ACNGT 3 cut(s) 85, 926, 1355
BstC8I GCNNGC 2 cut(s) 419, 1165
BstDEI CTNAG 6 cut(s) 123, 413, 576, 823, 1268, 1627
BstEII GGTNACC 1 cut(s) 566
BstENI CCTNNNNNAGG 1 cut(s) 575
BstF5I GGATG 5 cut(s) 142, 847, 871, 1390, 1639
BstFNI CGCG 2 cut(s) 552, 748
BstKTI GATC 5 cut(s) 178, 412, 718, 1193, 1462
BstMAI GTCTC 3 cut(s) 91, 498, 1215
BstMBI GATC 5 cut(s) 175, 409, 715, 1190, 1459
BstMCI CGRYCG 1 cut(s) 746
BstMWI GCNNNNNNNGC 3 cut(s) 290, 1164, 1193
BstNI CCWGG 2 cut(s) 628, 852
BstNSI RCATGY 1 cut(s) 919
BstPI GGTNACC 1 cut(s) 566
BstSCI CCNGG 2 cut(s) 626, 850
BstSFI CTRYAG 3 cut(s) 471, 1194, 1566
BstUI CGCG 2 cut(s) 552, 748
BstV1I GCAGC 5 cut(s) 710, 817, 1171, 1481, 1538
BstV2I GAAGAC 1 cut(s) 941
BstX2I RGATCY 1 cut(s) 1190
BstYI RGATCY 1 cut(s) 1190
BsuRI GGCC 3 cut(s) 204, 1564, 1648
BtsCI GGATG 5 cut(s) 142, 847, 871, 1390, 1639
BtsIMutI CAGTG 3 cut(s) 275, 1212, 1360
BtuMI TCGCGA 1 cut(s) 552
BveI ACCTGC 1 cut(s) 1055
Cac8I GCNNGC 2 cut(s) 419, 1165
CaiI CAGNNNCTG 1 cut(s) 830
Cfr10I RCCGGY 1 cut(s) 741
Cfr13I GGNCC 3 cut(s) 202, 598, 1646
Csp6I GTAC 4 cut(s) 130, 804, 1036, 1663
CspAI ACCGGT 1 cut(s) 741
CviAII CATG 4 cut(s) 308, 454, 916, 1258
CviQI GTAC 4 cut(s) 130, 804, 1036, 1663
DdeI CTNAG 6 cut(s) 123, 413, 576, 823, 1268, 1627
DpnI GATC 5 cut(s) 177, 411, 717, 1192, 1461
DpnII GATC 5 cut(s) 175, 409, 715, 1190, 1459
DraI TTTAAA 2 cut(s) 172, 685
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
Ecl136II GAGCTC 1 cut(s) 988
Eco24I GRGCYC 1 cut(s) 990
Eco32I GATATC 2 cut(s) 426, 1369
Eco47I GGWCC 1 cut(s) 598
Eco53kI GAGCTC 1 cut(s) 988
Eco57I CTGAAG 3 cut(s) 9, 420, 1422
Eco88I CYCGRG 1 cut(s) 989
Eco91I GGTNACC 1 cut(s) 566
EcoICRI GAGCTC 1 cut(s) 988
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoO65I GGTNACC 1 cut(s) 566
EcoRII CCWGG 2 cut(s) 626, 850
EcoRV GATATC 2 cut(s) 426, 1369
EcoT22I ATGCAT 1 cut(s) 385
EcoT38I GRGCYC 1 cut(s) 990
FaeI CATG 4 cut(s) 311, 457, 919, 1261
FatI CATG 4 cut(s) 307, 453, 915, 1257
FauI CCCGC 1 cut(s) 741
Fnu4HI GCNGC 5 cut(s) 724, 831, 1185, 1470, 1527
FokI GGATG 5 cut(s) 149, 834, 878, 1397, 1646
FriOI GRGCYC 1 cut(s) 990
Fsp4HI GCNGC 5 cut(s) 724, 831, 1185, 1470, 1527
FspBI CTAG 2 cut(s) 642, 1550
GluI GCNGC 5 cut(s) 724, 831, 1185, 1470, 1527
GsaI CCCAGC 2 cut(s) 225, 998
GsuI CTGGAG 2 cut(s) 118, 237
HaeIII GGCC 3 cut(s) 204, 1564, 1648
HapII CCGG 2 cut(s) 482, 742
Hin1II CATG 4 cut(s) 311, 457, 919, 1261
HincII GTYRAC 4 cut(s) 337, 871, 913, 1351
HindII GTYRAC 4 cut(s) 337, 871, 913, 1351
HindIII AAGCTT 2 cut(s) 21, 676
HinfI GANTC 3 cut(s) 368, 520, 1076
HpaI GTTAAC 2 cut(s) 337, 1351
HpaII CCGG 2 cut(s) 482, 742
HphI GGTGA 6 cut(s) 315, 578, 644, 769, 980, 1454
Hpy166II GTNNAC 5 cut(s) 337, 871, 898, 913, 1351
Hpy188I TCNGA 7 cut(s) 79, 414, 799, 826, 839, 922, 1285
Hpy8I GTNNAC 5 cut(s) 337, 871, 898, 913, 1351
Hpy99I CGWCG 2 cut(s) 926, 1500
HpyAV CCTTC 2 cut(s) 103, 1521
HpyCH4III ACNGT 3 cut(s) 85, 926, 1355
HpyF10VI GCNNNNNNNGC 3 cut(s) 290, 1164, 1193
HpyF3I CTNAG 6 cut(s) 123, 413, 576, 823, 1268, 1627
Hsp92II CATG 4 cut(s) 311, 457, 919, 1261
Kpn2I TCCGGA 1 cut(s) 481
KspAI GTTAAC 2 cut(s) 337, 1351
Kzo9I GATC 5 cut(s) 175, 409, 715, 1190, 1459
LmnI GCTCC 4 cut(s) 218, 254, 859, 1099
Lsp1109I GCAGC 5 cut(s) 710, 817, 1171, 1481, 1538
LweI GCATC 1 cut(s) 1605
MaeI CTAG 2 cut(s) 642, 1550
MaeIII GTNAC 3 cut(s) 448, 566, 1387
MalI GATC 5 cut(s) 177, 411, 717, 1192, 1461
MboI GATC 5 cut(s) 175, 409, 715, 1190, 1459
MboII GAAGA 5 cut(s) 29, 426, 499, 898, 941
MflI RGATCY 1 cut(s) 1190
MhlI GDGCHC 1 cut(s) 990
MluCI AATT 5 cut(s) 589, 1278, 1289, 1572, 1603
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 4 cut(s) 945, 977, 983, 1122
MnlI CCTC 8 cut(s) 85, 112, 235, 700, 901, 970, 992, 1352
Mph1103I ATGCAT 1 cut(s) 385
MroI TCCGGA 1 cut(s) 481
MslI CAYNNNNRTG 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 1631
MspI CCGG 2 cut(s) 482, 742
MspR9I CCNGG 2 cut(s) 628, 852
Mva1269I GAATGC 1 cut(s) 383
MvaI CCWGG 2 cut(s) 628, 852
MvnI CGCG 2 cut(s) 552, 748
MwoI GCNNNNNNNGC 3 cut(s) 290, 1164, 1193
NdeII GATC 5 cut(s) 175, 409, 715, 1190, 1459
NlaIII CATG 4 cut(s) 311, 457, 919, 1261
NlaIV GGNNCC 4 cut(s) 203, 1095, 1613, 1647
NmuCI GTSAC 2 cut(s) 566, 1387
NruI TCGCGA 1 cut(s) 552
NsiI ATGCAT 1 cut(s) 385
NspI RCATGY 1 cut(s) 919
PaeR7I CTCGAG 1 cut(s) 989
PaqCI CACCTGC 1 cut(s) 1055
PciI ACATGT 1 cut(s) 915
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 520, 1076
PfoI TCCNGGA 1 cut(s) 850
PinAI ACCGGT 1 cut(s) 741
PkrI GCNGC 5 cut(s) 725, 832, 1186, 1471, 1528
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
PscI ACATGT 1 cut(s) 915
PshBI ATTAAT 1 cut(s) 1581
PsiI TTATAA 1 cut(s) 147
Psp124BI GAGCTC 1 cut(s) 990
Psp6I CCWGG 2 cut(s) 626, 850
PspEI GGTNACC 1 cut(s) 566
PspFI CCCAGC 2 cut(s) 221, 994
PspGI CCWGG 2 cut(s) 626, 850
PspN4I GGNNCC 4 cut(s) 203, 1095, 1613, 1647
PspPI GGNCC 3 cut(s) 202, 598, 1646
PspXI VCTCGAGB 1 cut(s) 989
PsrI GAACNNNNNNTAC 2 cut(s) 1500, 1532
PstI CTGCAG 2 cut(s) 475, 1198
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 1 cut(s) 1190
RruI TCGCGA 1 cut(s) 552
RsaI GTAC 4 cut(s) 131, 805, 1037, 1664
RsaNI GTAC 4 cut(s) 130, 804, 1036, 1663
RseI CAYNNNNRTG 1 cut(s) 312
SacI GAGCTC 1 cut(s) 990
SatI GCNGC 5 cut(s) 724, 831, 1185, 1470, 1527
Sau3AI GATC 5 cut(s) 175, 409, 715, 1190, 1459
Sau96I GGNCC 3 cut(s) 202, 598, 1646
ScaI AGTACT 1 cut(s) 131
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 628, 852
SduI GDGCHC 1 cut(s) 990
SfaNI GCATC 1 cut(s) 1605
SfcI CTRYAG 3 cut(s) 471, 1194, 1566
Sfr274I CTCGAG 1 cut(s) 989
SinI GGWCC 1 cut(s) 598
SlaI CTCGAG 1 cut(s) 989
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 2 cut(s) 524, 989
SmoI CTYRAG 2 cut(s) 524, 989
Sse9I AATT 5 cut(s) 589, 1278, 1289, 1572, 1603
SsiI CCGC 4 cut(s) 748, 769, 1274, 1631
SspI AATATT 3 cut(s) 511, 1305, 1657
SspMI CTAG 2 cut(s) 642, 1550
SstI GAGCTC 1 cut(s) 990
StyD4I CCNGG 2 cut(s) 626, 850
TaaI ACNGT 3 cut(s) 85, 926, 1355
TaqI TCGA 6 cut(s) 959, 990, 1236, 1498, 1534, 1554
TasI AATT 5 cut(s) 589, 1278, 1289, 1572, 1603
TatI WGTACW 2 cut(s) 129, 1662
TfiI GAWTC 2 cut(s) 520, 1076
TscAI CASTG 3 cut(s) 282, 1219, 1360
TseFI GTSAC 2 cut(s) 566, 1387
TseI GCWGC 5 cut(s) 723, 830, 1184, 1469, 1526
Tsp45I GTSAC 2 cut(s) 566, 1387
TspDTI ATGAA 4 cut(s) 126, 776, 1434, 1521
TspGWI ACGGA 1 cut(s) 1397
TspRI CASTG 3 cut(s) 282, 1219, 1360
VpaK11BI GGWCC 1 cut(s) 598
VspI ATTAAT 1 cut(s) 1581
XagI CCTNNNNNAGG 1 cut(s) 575
XapI RAATTY 2 cut(s) 1289, 1572
XceI RCATGY 1 cut(s) 919
XcmI CCANNNNNNNNNTGG 1 cut(s) 212
XhoI CTCGAG 1 cut(s) 989
XspI CTAG 2 cut(s) 642, 1550
ZrmI AGTACT 1 cut(s) 131
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.