MD08G1065700.v1.1

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008272
Physical Location & Seq
Reverse (-)
5250623 .. 5255146
4524 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1065700.v1.1.491

Sequence Viewer

Length: 1680 bp
ATGGCGCCCACTGTCAAAGTAGTTTCGGAGCAAGAGCAAGCAGCCCCAAGGCTGCAGATTTACTCCACCTCTGATACTCTCTCTTCTTTCTGGAGAGAAAAGTATGAAAAAGATGCCAAGAAGTACTGGGATGTGTTTTACAAGCGGCATCAAGACAAGTTCTTTAAAGATCGGCACTACCTGGACAAGGAGTGGGGCAAATATTTCTCGGGCGCTGGGCGGAAAGTTATTTTAGAGGTTGGCTGTGGAACTGGAAACACTATTTTCCCACTGGTAACGATGTATGCAGATGTTTTTGTCCATGCATGCGATTTTTCAACAAATGCTATTAACTTGGTTAAGACGCATAAAGACTTCACAGATACCCGTGTTAGCGCATTTGTTTGTGATCCTACCATTGATGATCTAAGCAAGCAGATTTCTTCATCGTCCGTGGATATTGTAACCATGATTTTTGTGTTATCTGCAGTGTCTCCAGAGAAGATGCCTCTGTTACTGAAGAATATTAAGAAAGTTCTCAAGCCAAATGGTTGTGTGCTGTTTCGTGACTACGCTACTGGTGACCTTGCTCAGGAAAGACTGAAATGCAAGGACCAAAAGATAAGTGAAAACTTTTATGTCAGGGGTGATGGCACTCGTGCTTTCTACTTTGCTAATGAGTTCTTGACAAGCTTATTTAAAGATAATGGTTTTGATGTCGAAGAACTTGATTTGTGCTGCAAACAAGTTGAGAACCGGTCAAGGGAGTTGGTTATGAATCGACGTTGGGTTCAAGCCGTATTCCGCCTTTCAGACGATACAAAGTCCTCTACCAAAACGGAAGCAGCAACCAAAGTAGATCATCTTGGTCAAGAGAAAAGCGAGCTAGAGGTCAATGAGAATATCTTGAAGAAACCTGTGAATGATTTGGATGTTGACATTTCCGACGGTGTGGCGGTGGACATGTTTGGTGTCTTACCTTCCAATGACAACGAGGTAATTGAGCTAGAGCTAGGTGATTGGACTTGCAAGATTGAAGTGTTATCCAAAGTGTATCAACACACTTGCAAATCAACTGGTTTAATGTTGTGGGAATCAGCTCGGTTCATGGCTTCTGTTTTAACCAGAAATCCAACTATTGTCAGCGGGAAAAAGGTGTTGGAGTTGGGGTGTGGCTCCGGGGGCATTTGCTCTATGGTTGCCGCTAGATCTGCTGACCTTGTGTTTGCGACTGATGGTGACTCAAAAGCACTTGACCTACTGATGCAAAATGTCAAGTCGAATCTTAGACCGCCATTTCTGGACAAACTGATTACAAGAAGATTAGTGTGGGGAAATAGAGATCATATAGAAGCCATCAAGGAAGCTAATTCTCAAGGTTTTGATATCATCCTCGGCACAGACGTCACTTATGTAGCTGAAGCAACCTTGCCCTTATTTCAAACTGCAAAGGAGTTAATTTCATCGGACAGAAGCACCGCTGAGGATTTCAAACCGGCCCTAATCCTTTGTCATATTTTCCGTCGGGTAGATGAGCCTTCCATACTTTCAGCTGCATCCAAATTCGGGTTTAAGCTAGTCGATAAGTGGCCTATAGAAACTCCTCCTTCATCGCAAAGCATCATCAAATCTTGGTTCCCTGAGAATGGTTCGGAGAATGTCGAGAGGGGAGCATTACATATCCTGTACTTCCACGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_16 Methyltransf_23 Methyltransf_25

Protein Analysis

560

Amino Acids

63.0

Weight (kDa)

5.92

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 72 - 235 2.3e-12 Methyltransferase domain
Methyltransf_31 PF13847 75 - 199 1.1e-10 Methyltransferase domain
Methyltransf_25 PF13649 77 - 177 1.5e-13 Methyltransferase domain
Methyltransf_12 PF08242 78 - 178 2.2e-15 Methyltransferase domain
Methyltransf_11 PF08241 78 - 181 3.1e-10 Methyltransferase domain
Methyltransf_16 PF10294 344 - 484 1.4e-13 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1386
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 8 cut(s) 145, 220, 784, 935, 1125, 1182, 1271, 1458
AclWI GGATC 1 cut(s) 383
AcsI RAATTY 1 cut(s) 1541
AcuI CTGAAG 2 cut(s) 518, 1419
AcyI GRCGYC 2 cut(s) 5, 1383
AfaI GTAC 2 cut(s) 125, 1667
AfiI CCNNNNNNNGG 5 cut(s) 187, 571, 742, 1545, 1625
AflIII ACRYGT 1 cut(s) 942
AgeI ACCGGT 1 cut(s) 735
AgsI TTSAA 6 cut(s) 318, 773, 889, 1016, 1421, 1471
AjnI CCWGG 1 cut(s) 180
AjuI GAANNNNNNNTTGG 2 cut(s) 1121, 1153
AluBI AGCT 9 cut(s) 672, 865, 985, 991, 1079, 1346, 1397, 1532, 1555
AluI AGCT 9 cut(s) 672, 865, 985, 991, 1079, 1346, 1397, 1532, 1555
Alw26I GTCTC 1 cut(s) 477
AlwI GGATC 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 208
AoxI GGCC 2 cut(s) 1476, 1568
ApeKI GCWGC 5 cut(s) 41, 52, 717, 824, 1532
ApoI RAATTY 1 cut(s) 1541
ArsI GACNNNNNNTTYG 2 cut(s) 1187, 1219
AsiGI ACCGGT 1 cut(s) 735
AspLEI GCGC 3 cut(s) 7, 215, 377
AspS9I GGNCC 2 cut(s) 592, 1477
AsuC2I CCSGG 1 cut(s) 1159
AsuHPI GGTGA 4 cut(s) 572, 638, 1007, 1229
AvaI CYCGRG 1 cut(s) 208
AvaII GGWCC 1 cut(s) 592
BaeI ACNNNNGTAYC 2 cut(s) 354, 387
BanI GGYRCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 636
BbvCI CCTCAGC 1 cut(s) 1461
BbvI GCAGC 5 cut(s) 39, 53, 704, 836, 1519
BccI CCATC 3 cut(s) 623, 1208, 1343
BceAI ACGGC 1 cut(s) 761
BciT130I CCWGG 1 cut(s) 182
BcnI CCSGG 1 cut(s) 1159
BcoDI GTCTC 1 cut(s) 477
BfaI CTAG 5 cut(s) 866, 986, 992, 1185, 1556
BfmI CTRYAG 3 cut(s) 53, 465, 1572
BfoI RGCGCY 2 cut(s) 8, 216
BglII AGATCT 1 cut(s) 1187
BisI GCNGC 7 cut(s) 42, 53, 146, 718, 825, 1182, 1533
BlsI GCNGC 7 cut(s) 43, 54, 147, 719, 826, 1183, 1534
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 2 cut(s) 182, 1159
Bme18I GGWCC 1 cut(s) 592
BmeT110I CYCGRG 1 cut(s) 208
BmgT120I GGNCC 2 cut(s) 592, 1477
BmiI GGNNCC 3 cut(s) 6, 1156, 1616
BmrFI CCNGG 2 cut(s) 182, 1159
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 6 cut(s) 103, 157, 474, 1233, 1544, 1608
BmuI ACTGGG 1 cut(s) 136
BpmI CTGGAG 2 cut(s) 112, 459
Bpu10I CCTNAGC 2 cut(s) 570, 1461
BpuEI CTTGAG 2 cut(s) 503, 1338
BpuMI CCSGG 1 cut(s) 1159
BsaHI GRCGYC 2 cut(s) 5, 1383
BsaJI CCNNGG 4 cut(s) 47, 432, 1158, 1372
BsaWI WCCGGW 1 cut(s) 735
BsaXI ACNNNNNCTCC 2 cut(s) 1133, 1163
Bsc4I CCNNNNNNNGG 5 cut(s) 187, 571, 742, 1545, 1625
Bse118I RCCGGY 2 cut(s) 735, 1474
Bse1I ACTGG 5 cut(s) 131, 256, 276, 562, 1060
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 4 cut(s) 47, 432, 1158, 1372
BseGI GGATG 4 cut(s) 136, 916, 1368, 1535
BseLI CCNNNNNNNGG 5 cut(s) 187, 571, 742, 1545, 1625
BseMII CTCAG 3 cut(s) 584, 1452, 1611
BseNI ACTGG 5 cut(s) 131, 256, 276, 562, 1060
BseRI GAGGAG 1 cut(s) 1572
BseXI GCAGC 5 cut(s) 39, 53, 704, 836, 1519
BseYI CCCAGC 1 cut(s) 215
BshFI GGCC 2 cut(s) 1478, 1570
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 735
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 3 cut(s) 736, 1158, 1475
BslI CCNNNNNNNGG 5 cut(s) 187, 571, 742, 1545, 1625
BsmAI GTCTC 1 cut(s) 477
BsnI GGCC 2 cut(s) 1478, 1570
BsoBI CYCGRG 1 cut(s) 208
Bsp143I GATC 6 cut(s) 169, 388, 403, 838, 1187, 1321
BspACI CCGC 8 cut(s) 145, 220, 784, 935, 1125, 1182, 1271, 1458
BspANI GGCC 2 cut(s) 1478, 1570
BspCNI CTCAG 3 cut(s) 583, 1453, 1612
BspLI GGNNCC 3 cut(s) 6, 1156, 1616
BspMAI CTGCAG 2 cut(s) 57, 469
BspPI GGATC 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 2 cut(s) 735, 1474
BsrI ACTGG 5 cut(s) 131, 256, 276, 562, 1060
BssAI RCCGGY 2 cut(s) 735, 1474
BssECI CCNNGG 4 cut(s) 47, 432, 1158, 1372
BssMI GATC 6 cut(s) 169, 388, 403, 838, 1187, 1321
BssNI GRCGYC 2 cut(s) 5, 1383
BssSI CACGAG 1 cut(s) 636
BssT1I CCWWGG 1 cut(s) 47
Bst2BI CACGAG 1 cut(s) 636
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 2 cut(s) 13, 929
Bst6I CTCTTC 1 cut(s) 88
BstACI GRCGYC 2 cut(s) 5, 1383
BstC8I GCNNGC 4 cut(s) 39, 307, 413, 863
BstDEI CTNAG 5 cut(s) 407, 570, 1265, 1461, 1620
BstDSI CCRYGG 1 cut(s) 432
BstEII GGTNACC 1 cut(s) 560
BstENI CCTNNNNNAGG 2 cut(s) 185, 569
BstF5I GGATG 4 cut(s) 136, 916, 1368, 1535
BstH2I RGCGCY 2 cut(s) 8, 216
BstHHI GCGC 3 cut(s) 7, 215, 377
BstKTI GATC 6 cut(s) 172, 391, 406, 841, 1190, 1324
BstMAI GTCTC 1 cut(s) 477
BstMBI GATC 6 cut(s) 169, 388, 403, 838, 1187, 1321
BstMWI GCNNNNNNNGC 2 cut(s) 1161, 1190
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 2 cut(s) 309, 946
BstPI GGTNACC 1 cut(s) 560
BstSCI CCNGG 2 cut(s) 180, 1157
BstSFI CTRYAG 3 cut(s) 53, 465, 1572
BstV1I GCAGC 5 cut(s) 39, 53, 704, 836, 1519
BstX2I RGATCY 1 cut(s) 1187
BstYI RGATCY 1 cut(s) 1187
BsuRI GGCC 2 cut(s) 1478, 1570
BtgI CCRYGG 1 cut(s) 432
BtgZI GCGATG 1 cut(s) 1575
BtsCI GGATG 4 cut(s) 136, 916, 1368, 1535
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 3 cut(s) 9, 269, 474
Cac8I GCNNGC 4 cut(s) 39, 307, 413, 863
CfoI GCGC 3 cut(s) 7, 215, 377
Cfr10I RCCGGY 2 cut(s) 735, 1474
Cfr13I GGNCC 2 cut(s) 592, 1477
CseI GACGC 1 cut(s) 352
Csp6I GTAC 2 cut(s) 124, 1666
CspAI ACCGGT 1 cut(s) 735
CviAII CATG 5 cut(s) 302, 306, 448, 943, 1087
CviQI GTAC 2 cut(s) 124, 1666
DdeI CTNAG 5 cut(s) 407, 570, 1265, 1461, 1620
DinI GGCGCC 1 cut(s) 6
DpnI GATC 6 cut(s) 171, 390, 405, 840, 1189, 1323
DpnII GATC 6 cut(s) 169, 388, 403, 838, 1187, 1321
DraI TTTAAA 2 cut(s) 166, 679
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
EciI GGCGGA 2 cut(s) 235, 773
Eco130I CCWWGG 1 cut(s) 47
Eco32I GATATC 1 cut(s) 1366
Eco47I GGWCC 1 cut(s) 592
Eco57I CTGAAG 2 cut(s) 518, 1419
Eco88I CYCGRG 1 cut(s) 208
Eco91I GGTNACC 1 cut(s) 560
EcoNI CCTNNNNNAGG 2 cut(s) 185, 569
EcoO65I GGTNACC 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 180
EcoRV GATATC 1 cut(s) 1366
EcoT14I CCWWGG 1 cut(s) 47
EcoT22I ATGCAT 1 cut(s) 307
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 5 cut(s) 305, 309, 451, 946, 1090
FatI CATG 5 cut(s) 301, 305, 447, 942, 1086
FauI CCCGC 1 cut(s) 1118
Fnu4HI GCNGC 7 cut(s) 42, 53, 146, 718, 825, 1182, 1533
FokI GGATG 4 cut(s) 143, 923, 1355, 1522
Fsp4HI GCNGC 7 cut(s) 42, 53, 146, 718, 825, 1182, 1533
FspBI CTAG 5 cut(s) 866, 986, 992, 1185, 1556
GlaI GCGC 3 cut(s) 6, 214, 376
GluI GCNGC 7 cut(s) 42, 53, 146, 718, 825, 1182, 1533
GsaI CCCAGC 1 cut(s) 219
GsuI CTGGAG 2 cut(s) 112, 459
HaeII RGCGCY 2 cut(s) 8, 216
HaeIII GGCC 2 cut(s) 1478, 1570
HapII CCGG 3 cut(s) 736, 1158, 1475
HgaI GACGC 1 cut(s) 352
HhaI GCGC 3 cut(s) 7, 215, 377
Hin1I GRCGYC 2 cut(s) 5, 1383
Hin1II CATG 5 cut(s) 305, 309, 451, 946, 1090
Hin6I GCGC 3 cut(s) 5, 213, 375
HinP1I GCGC 3 cut(s) 5, 213, 375
HincII GTYRAC 1 cut(s) 916
HindII GTYRAC 1 cut(s) 916
HindIII AAGCTT 1 cut(s) 670
HinfI GANTC 4 cut(s) 757, 1073, 1220, 1261
HpaII CCGG 3 cut(s) 736, 1158, 1475
HphI GGTGA 4 cut(s) 572, 638, 1007, 1229
Hpy166II GTNNAC 2 cut(s) 916, 940
Hpy188I TCNGA 6 cut(s) 28, 73, 793, 925, 1447, 1633
Hpy8I GTNNAC 2 cut(s) 916, 940
Hpy99I CGWCG 3 cut(s) 765, 929, 1506
HpyAV CCTTC 3 cut(s) 969, 1527, 1596
HpyCH4III ACNGT 2 cut(s) 13, 929
HpyCH4IV ACGT 2 cut(s) 763, 1383
HpyF10VI GCNNNNNNNGC 2 cut(s) 1161, 1190
HpyF3I CTNAG 5 cut(s) 407, 570, 1265, 1461, 1620
HpySE526I ACGT 2 cut(s) 763, 1383
Hsp92I GRCGYC 2 cut(s) 5, 1383
Hsp92II CATG 5 cut(s) 305, 309, 451, 946, 1090
HspAI GCGC 3 cut(s) 5, 213, 375
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 6 cut(s) 169, 388, 403, 838, 1187, 1321
LmnI GCTCC 3 cut(s) 28, 1160, 1649
Lsp1109I GCAGC 5 cut(s) 39, 53, 704, 836, 1519
LweI GCATC 6 cut(s) 103, 157, 474, 1233, 1544, 1608
MaeI CTAG 5 cut(s) 866, 986, 992, 1185, 1556
MaeII ACGT 2 cut(s) 763, 1383
MaeIII GTNAC 7 cut(s) 274, 442, 492, 545, 560, 1217, 1384
MalI GATC 6 cut(s) 171, 390, 405, 840, 1189, 1323
MboI GATC 6 cut(s) 169, 388, 403, 838, 1187, 1321
MboII GAAGA 7 cut(s) 75, 414, 493, 511, 713, 901, 1311
MflI RGATCY 1 cut(s) 1187
MluCI AATT 4 cut(s) 978, 1348, 1437, 1541
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 1214
MmeI TCCRAC 3 cut(s) 948, 1119, 1136
Mph1103I ATGCAT 1 cut(s) 307
MseI TTAA 9 cut(s) 165, 330, 339, 507, 678, 1061, 1100, 1436, 1551
MspA1I CMGCKG 3 cut(s) 1125, 1460, 1532
MspI CCGG 3 cut(s) 736, 1158, 1475
MspR9I CCNGG 2 cut(s) 182, 1159
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 2 cut(s) 1161, 1190
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 1159
NdeII GATC 6 cut(s) 169, 388, 403, 838, 1187, 1321
NlaIII CATG 5 cut(s) 305, 309, 451, 946, 1090
NlaIV GGNNCC 3 cut(s) 6, 1156, 1616
NmeAIII GCCGAG 1 cut(s) 1353
NmuCI GTSAC 4 cut(s) 545, 560, 1217, 1384
NsiI ATGCAT 1 cut(s) 307
NspI RCATGY 2 cut(s) 309, 946
PaeI GCATGC 1 cut(s) 309
PciI ACATGT 1 cut(s) 942
PcsI WCGNNNNNNNCGW 1 cut(s) 1380
PfeI GAWTC 3 cut(s) 757, 1073, 1261
PinAI ACCGGT 1 cut(s) 735
PkrI GCNGC 7 cut(s) 43, 54, 147, 719, 826, 1183, 1534
PleI GAGTC 1 cut(s) 1214
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 1214
PscI ACATGT 1 cut(s) 942
Psp6I CCWGG 1 cut(s) 180
PspEI GGTNACC 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 215
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 3 cut(s) 6, 1156, 1616
PspPI GGNCC 2 cut(s) 592, 1477
PstI CTGCAG 2 cut(s) 57, 469
PsuI RGATCY 1 cut(s) 1187
PvuII CAGCTG 1 cut(s) 1532
RsaI GTAC 2 cut(s) 125, 1667
RsaNI GTAC 2 cut(s) 124, 1666
SaqAI TTAA 9 cut(s) 165, 330, 339, 507, 678, 1061, 1100, 1436, 1551
SatI GCNGC 7 cut(s) 42, 53, 146, 718, 825, 1182, 1533
Sau3AI GATC 6 cut(s) 169, 388, 403, 838, 1187, 1321
Sau96I GGNCC 2 cut(s) 592, 1477
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 1 cut(s) 1214
ScrFI CCNGG 2 cut(s) 182, 1159
SfaNI GCATC 6 cut(s) 103, 157, 474, 1233, 1544, 1608
SfcI CTRYAG 3 cut(s) 53, 465, 1572
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 592
SmlI CTYRAG 2 cut(s) 518, 1353
SmoI CTYRAG 2 cut(s) 518, 1353
SphI GCATGC 1 cut(s) 309
Sse9I AATT 4 cut(s) 978, 1348, 1437, 1541
SsiI CCGC 8 cut(s) 145, 220, 784, 935, 1125, 1182, 1271, 1458
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 2 cut(s) 203, 505
SspMI CTAG 5 cut(s) 866, 986, 992, 1185, 1556
StyD4I CCNGG 2 cut(s) 180, 1157
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 2 cut(s) 13, 929
TaiI ACGT 2 cut(s) 766, 1386
TaqI TCGA 5 cut(s) 699, 760, 1259, 1560, 1641
TasI AATT 4 cut(s) 978, 1348, 1437, 1541
TatI WGTACW 2 cut(s) 123, 1665
TauI GCSGC 2 cut(s) 148, 1184
TfiI GAWTC 3 cut(s) 757, 1073, 1261
Tru1I TTAA 9 cut(s) 165, 330, 339, 507, 678, 1061, 1100, 1436, 1551
Tru9I TTAA 9 cut(s) 165, 330, 339, 507, 678, 1061, 1100, 1436, 1551
TscAI CASTG 3 cut(s) 16, 276, 474
TseFI GTSAC 4 cut(s) 545, 560, 1217, 1384
TseI GCWGC 5 cut(s) 41, 52, 717, 824, 1532
Tsp45I GTSAC 4 cut(s) 545, 560, 1217, 1384
TspDTI ATGAA 6 cut(s) 120, 414, 770, 1075, 1431, 1578
TspGWI ACGGA 3 cut(s) 421, 833, 1490
TspRI CASTG 3 cut(s) 16, 276, 474
VpaK11BI GGWCC 1 cut(s) 592
XagI CCTNNNNNAGG 2 cut(s) 185, 569
XapI RAATTY 1 cut(s) 1541
XceI RCATGY 2 cut(s) 309, 946
XspI CTAG 5 cut(s) 866, 986, 992, 1185, 1556
ZraI GACGTC 1 cut(s) 1384
ZrmI AGTACT 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.