FvH4_2g32160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015119
Physical Location & Seq
Forward (+)
24450338 .. 24452060
1723 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32160.t2

Sequence Viewer

Length: 771 bp
ATGGAGGGAGGAGAGAGGGTGGGATCGGGTGGGACGAGGTACACGCTGAGGCCGGAAAGAATAAGCAACGAGGACATACTGTTCTGTGTGGATGTCGACCCGGAGTGTATGTCGGAGATGCGACCCGGCCCAAATGGCCGGGCCCTGACCCGATTGGAGTCCGTCAAGCAAGCCATCCTTCTCTTCATAAACGCCAAGCTCACCATCAATCCCGAACACCGGTTCGCCTTCTCTGTTCTGTCAAAGTCCGCGTCTTGGATACGGAAAGAGTTTAATAGTGATGTTGAGTCTGCTAAGGCTGCATTTCGAGCGATGTATGCCACTTCATCAACTGGGCATGCAGATCTTACCACTCTATTTCGAATGGCGGCTCATGAAGCAAAGAAATCCCGGGCGCAGAAGAGAATATTGAGGGTGATTCTTATCTACTGCAGATCATCGCTGCAACCACAACATCAGTGGCCTGTTAATCAGAAAATCTTCACTATGGATGTGCTTTACCTTCATGACAAGCCTGGACCCGACAACTGCCCCCAGCTGGTGTATGACAAACTTGTGGAAGCCCTCGAACATGTTACTGAATTTGAGGGCTACATCTTGGAGAGCGGTCAAGGGTTATCACGAACCCTCTTCCGTCACATGTCTATGCTGCTGTCACACCCTCAGCAGCGTTGCTCACAAGATTACTTGGACATACCTAGGTCTATCGCAAAAAGGTTGCCAGCAGACTCAACACCCAGTGACGATAGTATATCTGTATCCAGCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

28.93

Weight (kDa)

8.25

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015119)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32960
fragaria_vesca FvH4_2g32160 FvH4_2g32160
malus_domestica MD08G1073300.v1.1
prunus_persica Prupe.1G414700_v2.0.a1 Prupe.6G150100_v2.0.a1
pyrus_communis pycom08g06070
rosa_chinensis RchiOBHm_Chr6g0310521
rosa_laevigata RLG00000010476
rosa_roxburghii Rroxscaffold_7G00158460
rosa_rugosa Rorug06G0381200
rosa_samantha Rh6AG495100 Rh6BG504700 Rh6CG510000 Rh6DG496000
rosa_wichuraiana Rw6G043060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 606
AccI GTMKAC 1 cut(s) 96
AccII CGCG 1 cut(s) 251
AciI CCGC 3 cut(s) 249, 368, 606
AclWI GGATC 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 581
AdeI CACNNNGTG 1 cut(s) 740
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 3 cut(s) 219, 255, 538
AflIII ACRYGT 2 cut(s) 571, 639
AgeI ACCGGT 1 cut(s) 219
AjnI CCWGG 1 cut(s) 514
AloI GAACNNNNNNTCC 2 cut(s) 65, 97
AluBI AGCT 2 cut(s) 199, 538
AluI AGCT 2 cut(s) 199, 538
AlwI GGATC 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 390
AoxI GGCC 5 cut(s) 50, 127, 136, 141, 461
ApaI GGGCCC 1 cut(s) 145
ApeKI GCWGC 4 cut(s) 299, 442, 649, 667
ApoI RAATTY 1 cut(s) 581
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AsiGI ACCGGT 1 cut(s) 219
Asp700I GAANNNNTTC 1 cut(s) 479
AspA2I CCTAGG 1 cut(s) 698
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 4 cut(s) 128, 141, 142, 518
AsuC2I CCSGG 5 cut(s) 101, 126, 140, 391, 392
AsuHPI GGTGA 2 cut(s) 193, 427
AsuII TTCGAA 1 cut(s) 361
AvaI CYCGRG 1 cut(s) 390
AvaII GGWCC 1 cut(s) 518
AvrII CCTAGG 1 cut(s) 698
BaeGI GKGCMC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 145
BbvCI CCTCAGC 2 cut(s) 47, 663
BbvI GCAGC 4 cut(s) 286, 429, 636, 679
BccI CCATC 2 cut(s) 182, 212
BciT130I CCWGG 1 cut(s) 516
BciVI GTATCC 2 cut(s) 252, 769
BcnI CCSGG 5 cut(s) 101, 126, 140, 391, 392
BfaI CTAG 1 cut(s) 699
BfmI CTRYAG 1 cut(s) 430
BfuI GTATCC 2 cut(s) 252, 769
BglI GCCNNNNNGGC 1 cut(s) 135
BglII AGATCT 1 cut(s) 343
BisI GCNGC 5 cut(s) 300, 369, 443, 650, 668
BlnI CCTAGG 1 cut(s) 698
BlsI GCNGC 5 cut(s) 301, 370, 444, 651, 669
Bme1390I CCNGG 6 cut(s) 101, 126, 140, 391, 392, 516
Bme18I GGWCC 1 cut(s) 518
BmeT110I CYCGRG 1 cut(s) 390
BmgT120I GGNCC 4 cut(s) 128, 141, 142, 518
BmiI GGNNCC 2 cut(s) 143, 520
BmrFI CCNGG 6 cut(s) 101, 126, 140, 391, 392, 516
BmrI ACTGGG 2 cut(s) 342, 732
BmsI GCATC 1 cut(s) 108
BmuI ACTGGG 2 cut(s) 342, 732
Bpu10I CCTNAGC 3 cut(s) 47, 294, 663
Bpu14I TTCGAA 1 cut(s) 361
BpuMI CCSGG 5 cut(s) 101, 126, 140, 391, 392
BsaBI GATNNNNATC 1 cut(s) 422
BsaJI CCNNGG 2 cut(s) 390, 698
BsaWI WCCGGW 1 cut(s) 219
BsaXI ACNNNNNCTCC 3 cut(s) 33, 95, 125
Bsc4I CCNNNNNNNGG 3 cut(s) 219, 255, 538
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 2 cut(s) 337, 738
Bse8I GATNNNNATC 1 cut(s) 422
BseBI CCWGG 1 cut(s) 516
BseDI CCNNGG 2 cut(s) 390, 698
BseGI GGATG 3 cut(s) 97, 174, 496
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 3 cut(s) 219, 255, 538
BseMII CTCAG 2 cut(s) 38, 677
BseNI ACTGG 2 cut(s) 337, 738
BseRI GAGGAG 1 cut(s) 24
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 4 cut(s) 286, 429, 636, 679
BseYI CCCAGC 1 cut(s) 534
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 5 cut(s) 52, 129, 138, 143, 463
BshTI ACCGGT 1 cut(s) 219
BsiHKCI CYCGRG 1 cut(s) 390
BsiSI CCGG 6 cut(s) 53, 101, 126, 139, 220, 391
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 3 cut(s) 219, 255, 538
BsmFI GGGAC 1 cut(s) 46
BsnI GGCC 5 cut(s) 52, 129, 138, 143, 463
BsoBI CYCGRG 1 cut(s) 390
Bsp119I TTCGAA 1 cut(s) 361
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 145
Bsp143I GATC 3 cut(s) 23, 343, 434
BspACI CCGC 3 cut(s) 249, 368, 606
BspANI GGCC 5 cut(s) 52, 129, 138, 143, 463
BspCNI CTCAG 2 cut(s) 39, 676
BspFNI CGCG 1 cut(s) 251
BspHI TCATGA 2 cut(s) 373, 505
BspLI GGNNCC 2 cut(s) 143, 520
BspMAI CTGCAG 1 cut(s) 434
BspPI GGATC 1 cut(s) 31
BspT104I TTCGAA 1 cut(s) 361
BsrBI CCGCTC 1 cut(s) 606
BsrFI RCCGGY 1 cut(s) 219
BsrI ACTGG 2 cut(s) 337, 738
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 2 cut(s) 390, 698
BssMI GATC 3 cut(s) 23, 343, 434
BssT1I CCWWGG 1 cut(s) 698
Bst2UI CCWGG 1 cut(s) 516
Bst4CI ACNGT 1 cut(s) 81
Bst6I CTCTTC 3 cut(s) 188, 395, 635
BstBI TTCGAA 1 cut(s) 361
BstC8I GCNNGC 3 cut(s) 171, 339, 723
BstDEI CTNAG 3 cut(s) 47, 294, 663
BstF5I GGATG 3 cut(s) 97, 174, 496
BstFNI CGCG 1 cut(s) 251
BstHHI GCGC 1 cut(s) 397
BstKTI GATC 3 cut(s) 26, 346, 437
BstMBI GATC 3 cut(s) 23, 343, 434
BstMWI GCNNNNNNNGC 5 cut(s) 135, 299, 308, 317, 377
BstNI CCWGG 1 cut(s) 516
BstNSI RCATGY 3 cut(s) 341, 575, 643
BstSCI CCNGG 6 cut(s) 99, 124, 138, 389, 390, 514
BstSFI CTRYAG 1 cut(s) 430
BstSLI GKGCMC 1 cut(s) 145
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 4 cut(s) 286, 429, 636, 679
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BsuI GTATCC 2 cut(s) 252, 769
BsuRI GGCC 5 cut(s) 52, 129, 138, 143, 463
BtgZI GCGATG 2 cut(s) 326, 423
BtsCI GGATG 3 cut(s) 97, 174, 496
BtsIMutI CAGTG 2 cut(s) 464, 745
Cac8I GCNNGC 3 cut(s) 171, 339, 723
CciI TCATGA 2 cut(s) 373, 505
CfoI GCGC 1 cut(s) 397
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 4 cut(s) 128, 141, 142, 518
Cfr9I CCCGGG 1 cut(s) 390
CseI GACGC 1 cut(s) 240
Csp6I GTAC 1 cut(s) 40
CspAI ACCGGT 1 cut(s) 219
CspCI CAANNNNNGTGG 2 cut(s) 440, 475
CviAII CATG 5 cut(s) 338, 374, 506, 572, 640
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 3 cut(s) 47, 294, 663
DpnI GATC 3 cut(s) 25, 345, 436
DpnII GATC 3 cut(s) 23, 343, 434
DraIII CACNNNGTG 1 cut(s) 740
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 3 cut(s) 188, 395, 635
EarI CTCTTC 3 cut(s) 188, 395, 635
Eco130I CCWWGG 1 cut(s) 698
Eco24I GRGCYC 1 cut(s) 145
Eco47I GGWCC 1 cut(s) 518
Eco88I CYCGRG 1 cut(s) 390
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 698
EcoT38I GRGCYC 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 698
FaeI CATG 5 cut(s) 341, 377, 509, 575, 643
FalI AAGNNNNNCTT 2 cut(s) 162, 194
FaqI GGGAC 1 cut(s) 46
FatI CATG 5 cut(s) 337, 373, 505, 571, 639
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 5 cut(s) 300, 369, 443, 650, 668
FokI GGATG 3 cut(s) 104, 161, 503
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 5 cut(s) 300, 369, 443, 650, 668
FspBI CTAG 1 cut(s) 699
GlaI GCGC 1 cut(s) 396
GluI GCNGC 5 cut(s) 300, 369, 443, 650, 668
GsaI CCCAGC 1 cut(s) 538
HaeIII GGCC 5 cut(s) 52, 129, 138, 143, 463
HapII CCGG 6 cut(s) 53, 101, 126, 139, 220, 391
HgaI GACGC 1 cut(s) 240
HhaI GCGC 1 cut(s) 397
Hin1II CATG 5 cut(s) 341, 377, 509, 575, 643
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 4 cut(s) 158, 287, 418, 728
HpaII CCGG 6 cut(s) 53, 101, 126, 139, 220, 391
HphI GGTGA 2 cut(s) 193, 427
Hpy166II GTNNAC 2 cut(s) 42, 97
Hpy188I TCNGA 2 cut(s) 115, 474
Hpy188III TCNNGA 4 cut(s) 212, 374, 506, 621
Hpy8I GTNNAC 2 cut(s) 42, 97
HpyAV CCTTC 3 cut(s) 188, 238, 512
HpyCH4III ACNGT 1 cut(s) 81
HpyCH4V TGCA 4 cut(s) 302, 341, 432, 445
HpyF10VI GCNNNNNNNGC 5 cut(s) 135, 299, 308, 317, 377
HpyF3I CTNAG 3 cut(s) 47, 294, 663
Hsp92II CATG 5 cut(s) 341, 377, 509, 575, 643
HspAI GCGC 1 cut(s) 395
Kzo9I GATC 3 cut(s) 23, 343, 434
Lsp1109I GCAGC 4 cut(s) 286, 429, 636, 679
LweI GCATC 1 cut(s) 108
MaeI CTAG 1 cut(s) 699
MaeIII GTNAC 4 cut(s) 574, 635, 654, 740
MalI GATC 3 cut(s) 25, 345, 436
MbiI CCGCTC 1 cut(s) 606
MboI GATC 3 cut(s) 23, 343, 434
MboII GAAGA 4 cut(s) 175, 412, 472, 622
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 1 cut(s) 581
MlyI GAGTC 3 cut(s) 167, 296, 722
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 9 cut(s) 9, 30, 42, 64, 405, 575, 580, 638, 672
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 2 cut(s) 273, 468
MslI CAYNNNNRTG 1 cut(s) 644
MspA1I CMGCKG 1 cut(s) 538
MspI CCGG 6 cut(s) 53, 101, 126, 139, 220, 391
MspR9I CCNGG 6 cut(s) 101, 126, 140, 391, 392, 516
MvaI CCWGG 1 cut(s) 516
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 5 cut(s) 135, 299, 308, 317, 377
NciI CCSGG 5 cut(s) 101, 126, 140, 391, 392
NdeII GATC 3 cut(s) 23, 343, 434
NlaIII CATG 5 cut(s) 341, 377, 509, 575, 643
NlaIV GGNNCC 2 cut(s) 143, 520
NmuCI GTSAC 3 cut(s) 635, 654, 740
NspI RCATGY 3 cut(s) 341, 575, 643
NspV TTCGAA 1 cut(s) 361
PaeI GCATGC 1 cut(s) 341
PagI TCATGA 2 cut(s) 373, 505
PciI ACATGT 2 cut(s) 571, 639
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 479
PfeI GAWTC 1 cut(s) 418
PinAI ACCGGT 1 cut(s) 219
PkrI GCNGC 5 cut(s) 301, 370, 444, 651, 669
PleI GAGTC 3 cut(s) 166, 295, 722
PpsI GAGTC 3 cut(s) 166, 295, 722
PscI ACATGT 2 cut(s) 571, 639
Psp6I CCWGG 1 cut(s) 514
PspFI CCCAGC 1 cut(s) 534
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 2 cut(s) 143, 520
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 4 cut(s) 128, 141, 142, 518
PstI CTGCAG 1 cut(s) 434
PsuI RGATCY 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 644
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 2 cut(s) 273, 468
SatI GCNGC 5 cut(s) 300, 369, 443, 650, 668
Sau3AI GATC 3 cut(s) 23, 343, 434
Sau96I GGNCC 4 cut(s) 128, 141, 142, 518
SchI GAGTC 3 cut(s) 167, 296, 722
ScrFI CCNGG 6 cut(s) 101, 126, 140, 391, 392, 516
SduI GDGCHC 1 cut(s) 145
SetI ASST 7 cut(s) 41, 201, 504, 540, 700, 704, 719
SfaNI GCATC 1 cut(s) 108
SfcI CTRYAG 1 cut(s) 430
SfiI GGCCNNNNNGGCC 1 cut(s) 135
SfuI TTCGAA 1 cut(s) 361
SinI GGWCC 1 cut(s) 518
SmaI CCCGGG 1 cut(s) 392
SmiMI CAYNNNNRTG 1 cut(s) 644
SphI GCATGC 1 cut(s) 341
Sse9I AATT 1 cut(s) 581
SsiI CCGC 3 cut(s) 249, 368, 606
SspI AATATT 1 cut(s) 408
SspMI CTAG 1 cut(s) 699
StyD4I CCNGG 6 cut(s) 99, 124, 138, 389, 390, 514
StyI CCWWGG 1 cut(s) 698
TaaI ACNGT 1 cut(s) 81
TaqI TCGA 4 cut(s) 96, 307, 361, 567
TasI AATT 1 cut(s) 581
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 2 cut(s) 273, 468
Tru9I TTAA 2 cut(s) 273, 468
TscAI CASTG 2 cut(s) 464, 745
TseFI GTSAC 3 cut(s) 635, 654, 740
TseI GCWGC 4 cut(s) 299, 442, 649, 667
Tsp45I GTSAC 3 cut(s) 635, 654, 740
TspDTI ATGAA 4 cut(s) 175, 315, 390, 494
TspGWI ACGGA 3 cut(s) 151, 277, 623
TspMI CCCGGG 1 cut(s) 390
TspRI CASTG 2 cut(s) 464, 745
VpaK11BI GGWCC 1 cut(s) 518
XapI RAATTY 1 cut(s) 581
XceI RCATGY 3 cut(s) 341, 575, 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 456
XmaI CCCGGG 1 cut(s) 390
XmaJI CCTAGG 1 cut(s) 698
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.