MD08G1073300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015119
Physical Location & Seq
Forward (+)
5919221 .. 5921199
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1073300.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGAGGGAATAGTAGAGGGGGGCGGATCGGGTGCGGGCAGATACACGCTGCGGCCGGAAAGGCTGAGCAACGAGGACATACTGTTCTGCATAGACGTTGACCCAGAGTCGATGGTGGAGATGAAGGCTGCGATGGGCCCGAACGGGCGACCCTTAACCCGATTGGACTCGATCAAGCAAGCCATACTTCTCTTCATCAACGCCAAGCTTACCATCAATCCGGAACACCGATTTGCTTTCGCCACTCTCGGAAAGGCCGCGTCTTGGCTTCGAAGAGAGTTTAGCAGTGAAGTTGAGTCTGCTCATGCTGCACTTCGGATACTCTCAGCCATTCCTTCTTCTGGTAATGCAGATCTTACTGGTCTATTTCGAATAGCATCTCATGAAGCAAAGAAATCTCGCGCTCAAAACAGAATTTTGAGGGTGGTTCTGATCTACTGTAGATCGTCCGTACGACCGCAAAATAATTGGCCGGTAAACCAGAAACTCTTCACCCTGGATGTTGTTTACCTTCATGACAAGCCTGGACCTGACAACTGCCCCCAGGAGGTCTACGATGCACTTGTGGATTCCCTGGAACATGTTAGCGAATTCGAGGGATACATCTTAGAGAGTGGACAGGGATTAACACGAGTCCTCTTCCGTCACATGTGTGTGCTGCTATCGCACCCTCAGCAGCGATGCCCGCAAGACCACCTGGACATACCTAAGGCTCTCACAAAACGGTTGCTGGCAGCAGACACAGTGAACGGTGAAGATAGTGTAACTGTATCCGGGGGCAAATCCGATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
No domains found.

Protein Analysis

265

Amino Acids

29.21

Weight (kDa)

6.51

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015119)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32960
fragaria_vesca FvH4_2g32160 FvH4_2g32160
malus_domestica MD08G1073300.v1.1
prunus_persica Prupe.1G414700_v2.0.a1 Prupe.6G150100_v2.0.a1
pyrus_communis pycom08g06070
rosa_chinensis RchiOBHm_Chr6g0310521
rosa_laevigata RLG00000010476
rosa_roxburghii Rroxscaffold_7G00158460
rosa_rugosa Rorug06G0381200
rosa_samantha Rh6AG495100 Rh6BG504700 Rh6CG510000 Rh6DG496000
rosa_wichuraiana Rw6G043060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 552
AccII CGCG 2 cut(s) 260, 402
AccIII TCCGGA 1 cut(s) 220
AciI CCGC 6 cut(s) 24, 35, 52, 258, 458, 686
AclWI GGATC 1 cut(s) 34
AcoI YGGCCR 2 cut(s) 53, 470
AcsI RAATTY 2 cut(s) 414, 590
AfaI GTAC 1 cut(s) 453
AfiI CCNNNNNNNGG 3 cut(s) 264, 341, 547
AflIII ACRYGT 2 cut(s) 580, 648
AhdI GACNNNNNGTC 1 cut(s) 106
AjnI CCWGG 5 cut(s) 495, 523, 543, 573, 696
AleI CACNNNNGTG 1 cut(s) 651
AloI GAACNNNNNNTCC 2 cut(s) 68, 100
AluBI AGCT 1 cut(s) 208
AluI AGCT 1 cut(s) 208
AlwI GGATC 1 cut(s) 34
Aor13HI TCCGGA 1 cut(s) 220
AoxI GGCC 4 cut(s) 53, 136, 255, 470
ApaI GGGCCC 1 cut(s) 140
ApeKI GCWGC 6 cut(s) 49, 128, 308, 658, 676, 734
ApoI RAATTY 2 cut(s) 414, 590
Asp700I GAANNNNTTC 1 cut(s) 488
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 3 cut(s) 136, 137, 527
AsuC2I CCSGG 1 cut(s) 775
AsuHPI GGTGA 2 cut(s) 484, 764
AsuII TTCGAA 2 cut(s) 271, 370
AvaII GGWCC 1 cut(s) 527
AxyI CCTNAGG 1 cut(s) 708
BaeGI GKGCMC 1 cut(s) 140
BanII GRGCYC 1 cut(s) 140
BauI CACGAG 1 cut(s) 630
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 6 cut(s) 36, 115, 295, 645, 688, 746
BccI CCATC 3 cut(s) 106, 127, 221
BciT130I CCWGG 5 cut(s) 497, 525, 545, 575, 698
BciVI GTATCC 3 cut(s) 312, 593, 781
BcnI CCSGG 1 cut(s) 775
BfmI CTRYAG 1 cut(s) 439
BfuI GTATCC 3 cut(s) 312, 593, 781
BglI GCCNNNNNGGC 1 cut(s) 61
BglII AGATCT 1 cut(s) 352
BisI GCNGC 8 cut(s) 50, 53, 129, 258, 309, 659, 677, 735
BlpI GCTNAGC 1 cut(s) 65
BlsI GCNGC 8 cut(s) 51, 54, 130, 259, 310, 660, 678, 736
Bme1390I CCNGG 6 cut(s) 497, 525, 545, 575, 698, 775
Bme18I GGWCC 1 cut(s) 527
BmeRI GACNNNNNGTC 1 cut(s) 106
BmgT120I GGNCC 3 cut(s) 136, 137, 527
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 6 cut(s) 497, 525, 545, 575, 698, 775
BmsI GCATC 3 cut(s) 386, 547, 671
Bpu10I CCTNAGC 1 cut(s) 672
Bpu1102I GCTNAGC 1 cut(s) 65
Bpu14I TTCGAA 2 cut(s) 271, 370
BpuMI CCSGG 1 cut(s) 775
BsaJI CCNNGG 4 cut(s) 495, 543, 573, 774
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 3 cut(s) 264, 341, 547
Bse118I RCCGGY 1 cut(s) 472
Bse1I ACTGG 1 cut(s) 364
Bse21I CCTNAGG 1 cut(s) 708
BseAI TCCGGA 1 cut(s) 220
BseBI CCWGG 5 cut(s) 497, 525, 545, 575, 698
BseDI CCNNGG 4 cut(s) 495, 543, 573, 774
BseGI GGATG 1 cut(s) 505
BseLI CCNNNNNNNGG 3 cut(s) 264, 341, 547
BseMII CTCAG 3 cut(s) 56, 339, 686
BseNI ACTGG 1 cut(s) 364
BseSI GKGCMC 1 cut(s) 140
BseX3I CGGCCG 1 cut(s) 53
BseXI GCAGC 6 cut(s) 36, 115, 295, 645, 688, 746
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 2 cut(s) 260, 402
Bsh1285I CGRYCG 2 cut(s) 56, 458
BshFI GGCC 4 cut(s) 55, 138, 257, 472
BsiEI CGRYCG 2 cut(s) 56, 458
BsiSI CCGG 4 cut(s) 56, 221, 473, 774
BsiWI CGTACG 1 cut(s) 451
BslI CCNNNNNNNGG 3 cut(s) 264, 341, 547
BsnI GGCC 4 cut(s) 55, 138, 257, 472
Bsp119I TTCGAA 2 cut(s) 271, 370
Bsp120I GGGCCC 1 cut(s) 136
Bsp1286I GDGCHC 1 cut(s) 140
Bsp13I TCCGGA 1 cut(s) 220
Bsp143I GATC 6 cut(s) 26, 171, 352, 432, 443, 787
Bsp1720I GCTNAGC 1 cut(s) 65
BspACI CCGC 6 cut(s) 24, 35, 52, 258, 458, 686
BspANI GGCC 4 cut(s) 55, 138, 257, 472
BspCNI CTCAG 3 cut(s) 57, 338, 685
BspEI TCCGGA 1 cut(s) 220
BspFNI CGCG 2 cut(s) 260, 402
BspHI TCATGA 2 cut(s) 382, 514
BspLI GGNNCC 1 cut(s) 138
BspPI GGATC 1 cut(s) 34
BspT104I TTCGAA 2 cut(s) 271, 370
BsrFI RCCGGY 1 cut(s) 472
BsrI ACTGG 1 cut(s) 364
BssAI RCCGGY 1 cut(s) 472
BssECI CCNNGG 4 cut(s) 495, 543, 573, 774
BssMI GATC 6 cut(s) 26, 171, 352, 432, 443, 787
BssSI CACGAG 1 cut(s) 630
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 5 cut(s) 497, 525, 545, 575, 698
Bst4CI ACNGT 6 cut(s) 84, 440, 726, 745, 752, 769
Bst6I CTCTTC 4 cut(s) 197, 268, 494, 644
BstBI TTCGAA 2 cut(s) 271, 370
BstC8I GCNNGC 4 cut(s) 37, 180, 686, 732
BstDEI CTNAG 5 cut(s) 65, 325, 607, 672, 708
BstF5I GGATG 1 cut(s) 505
BstFNI CGCG 2 cut(s) 260, 402
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 6 cut(s) 29, 174, 355, 435, 446, 790
BstMBI GATC 6 cut(s) 26, 171, 352, 432, 443, 787
BstMCI CGRYCG 2 cut(s) 56, 458
BstMWI GCNNNNNNNGC 5 cut(s) 61, 308, 664, 673, 685
BstNI CCWGG 5 cut(s) 497, 525, 545, 575, 698
BstNSI RCATGY 2 cut(s) 584, 652
BstSCI CCNGG 6 cut(s) 495, 523, 543, 573, 696, 773
BstSFI CTRYAG 1 cut(s) 439
BstSLI GKGCMC 1 cut(s) 140
BstUI CGCG 2 cut(s) 260, 402
BstV1I GCAGC 6 cut(s) 36, 115, 295, 645, 688, 746
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BstZI CGGCCG 1 cut(s) 53
Bsu36I CCTNAGG 1 cut(s) 708
BsuI GTATCC 3 cut(s) 312, 593, 781
BsuRI GGCC 4 cut(s) 55, 138, 257, 472
BtgZI GCGATG 2 cut(s) 146, 694
BtsCI GGATG 1 cut(s) 505
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 2 cut(s) 292, 750
Cac8I GCNNGC 4 cut(s) 37, 180, 686, 732
CciI TCATGA 2 cut(s) 382, 514
CfoI GCGC 1 cut(s) 404
Cfr10I RCCGGY 1 cut(s) 472
Cfr13I GGNCC 3 cut(s) 136, 137, 527
CseI GACGC 1 cut(s) 249
Csp6I GTAC 1 cut(s) 452
CviAII CATG 5 cut(s) 305, 383, 515, 581, 649
CviQI GTAC 1 cut(s) 452
DdeI CTNAG 5 cut(s) 65, 325, 607, 672, 708
DpnI GATC 6 cut(s) 28, 173, 354, 434, 445, 789
DpnII GATC 6 cut(s) 26, 171, 352, 432, 443, 787
DriI GACNNNNNGTC 1 cut(s) 106
EaeI YGGCCR 2 cut(s) 53, 470
EagI CGGCCG 1 cut(s) 53
Eam1104I CTCTTC 4 cut(s) 197, 268, 494, 644
Eam1105I GACNNNNNGTC 1 cut(s) 106
EarI CTCTTC 4 cut(s) 197, 268, 494, 644
EciI GGCGGA 1 cut(s) 39
EclXI CGGCCG 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 140
Eco47I GGWCC 1 cut(s) 527
Eco52I CGGCCG 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 708
EcoRI GAATTC 1 cut(s) 590
EcoRII CCWGG 5 cut(s) 495, 523, 543, 573, 696
EcoT38I GRGCYC 1 cut(s) 140
FaeI CATG 5 cut(s) 308, 386, 518, 584, 652
FaiI YATR 9 cut(s) 80, 92, 185, 306, 384, 516, 582, 650, 704
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FatI CATG 5 cut(s) 304, 382, 514, 580, 648
FauI CCCGC 2 cut(s) 28, 693
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 8 cut(s) 50, 53, 129, 258, 309, 659, 677, 735
FokI GGATG 1 cut(s) 512
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 8 cut(s) 50, 53, 129, 258, 309, 659, 677, 735
GlaI GCGC 1 cut(s) 403
GluI GCNGC 8 cut(s) 50, 53, 129, 258, 309, 659, 677, 735
HaeIII GGCC 4 cut(s) 55, 138, 257, 472
HapII CCGG 4 cut(s) 56, 221, 473, 774
HgaI GACGC 1 cut(s) 249
HhaI GCGC 1 cut(s) 404
Hin1II CATG 5 cut(s) 308, 386, 518, 584, 652
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 5 cut(s) 107, 167, 296, 569, 633
HpaII CCGG 4 cut(s) 56, 221, 473, 774
HphI GGTGA 2 cut(s) 484, 764
Hpy166II GTNNAC 6 cut(s) 100, 478, 508, 553, 617, 748
Hpy188I TCNGA 4 cut(s) 251, 318, 432, 787
Hpy188III TCNNGA 3 cut(s) 221, 383, 515
Hpy8I GTNNAC 6 cut(s) 100, 478, 508, 553, 617, 748
HpyAV CCTTC 3 cut(s) 118, 345, 521
HpyCH4III ACNGT 6 cut(s) 84, 440, 726, 745, 752, 769
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 4 cut(s) 90, 311, 350, 560
HpyF10VI GCNNNNNNNGC 5 cut(s) 61, 308, 664, 673, 685
HpyF3I CTNAG 5 cut(s) 65, 325, 607, 672, 708
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 5 cut(s) 308, 386, 518, 584, 652
HspAI GCGC 1 cut(s) 402
Kpn2I TCCGGA 1 cut(s) 220
Kzo9I GATC 6 cut(s) 26, 171, 352, 432, 443, 787
Lsp1109I GCAGC 6 cut(s) 36, 115, 295, 645, 688, 746
LweI GCATC 3 cut(s) 386, 547, 671
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 644, 763
MalI GATC 6 cut(s) 28, 173, 354, 434, 445, 789
MboI GATC 6 cut(s) 26, 171, 352, 432, 443, 787
MboII GAAGA 6 cut(s) 184, 285, 330, 481, 631, 767
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 1 cut(s) 140
MluCI AATT 3 cut(s) 414, 466, 590
MlyI GAGTC 4 cut(s) 116, 161, 305, 642
MnlI CCTC 7 cut(s) 10, 67, 414, 541, 589, 647, 681
MroI TCCGGA 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 2 cut(s) 155, 626
MslI CAYNNNNRTG 2 cut(s) 651, 653
MspI CCGG 4 cut(s) 56, 221, 473, 774
MspR9I CCNGG 6 cut(s) 497, 525, 545, 575, 698, 775
MvaI CCWGG 5 cut(s) 497, 525, 545, 575, 698
MvnI CGCG 2 cut(s) 260, 402
MwoI GCNNNNNNNGC 5 cut(s) 61, 308, 664, 673, 685
NciI CCSGG 1 cut(s) 775
NdeII GATC 6 cut(s) 26, 171, 352, 432, 443, 787
NlaIII CATG 5 cut(s) 308, 386, 518, 584, 652
NlaIV GGNNCC 1 cut(s) 138
NmuCI GTSAC 1 cut(s) 644
NspI RCATGY 2 cut(s) 584, 652
NspV TTCGAA 2 cut(s) 271, 370
OliI CACNNNNGTG 1 cut(s) 651
PagI TCATGA 2 cut(s) 382, 514
PciI ACATGT 2 cut(s) 580, 648
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 1 cut(s) 569
Pfl23II CGTACG 1 cut(s) 451
PkrI GCNGC 8 cut(s) 51, 54, 130, 259, 310, 660, 678, 736
PleI GAGTC 4 cut(s) 115, 161, 304, 641
PpsI GAGTC 4 cut(s) 115, 161, 304, 641
PscI ACATGT 2 cut(s) 580, 648
Psp6I CCWGG 5 cut(s) 495, 523, 543, 573, 696
PspGI CCWGG 5 cut(s) 495, 523, 543, 573, 696
PspLI CGTACG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 138
PspOMI GGGCCC 1 cut(s) 136
PspPI GGNCC 3 cut(s) 136, 137, 527
PsuI RGATCY 1 cut(s) 352
RsaI GTAC 1 cut(s) 453
RsaNI GTAC 1 cut(s) 452
RseI CAYNNNNRTG 2 cut(s) 651, 653
SaqAI TTAA 2 cut(s) 155, 626
SatI GCNGC 8 cut(s) 50, 53, 129, 258, 309, 659, 677, 735
Sau3AI GATC 6 cut(s) 26, 171, 352, 432, 443, 787
Sau96I GGNCC 3 cut(s) 136, 137, 527
SchI GAGTC 4 cut(s) 116, 161, 305, 642
ScrFI CCNGG 6 cut(s) 497, 525, 545, 575, 698, 775
SduI GDGCHC 1 cut(s) 140
SetI ASST 7 cut(s) 99, 210, 513, 532, 552, 699, 709
SfaNI GCATC 3 cut(s) 386, 547, 671
SfcI CTRYAG 1 cut(s) 439
SfuI TTCGAA 2 cut(s) 271, 370
SinI GGWCC 1 cut(s) 527
SmiMI CAYNNNNRTG 2 cut(s) 651, 653
Sse9I AATT 3 cut(s) 414, 466, 590
SsiI CCGC 6 cut(s) 24, 35, 52, 258, 458, 686
StyD4I CCNGG 6 cut(s) 495, 523, 543, 573, 696, 773
TaaI ACNGT 6 cut(s) 84, 440, 726, 745, 752, 769
TaiI ACGT 1 cut(s) 99
TaqI TCGA 5 cut(s) 110, 170, 271, 370, 594
TasI AATT 3 cut(s) 414, 466, 590
TauI GCSGC 2 cut(s) 55, 260
TfiI GAWTC 1 cut(s) 569
Tru1I TTAA 2 cut(s) 155, 626
Tru9I TTAA 2 cut(s) 155, 626
TscAI CASTG 2 cut(s) 292, 750
TseFI GTSAC 1 cut(s) 644
TseI GCWGC 6 cut(s) 49, 128, 308, 658, 676, 734
Tsp45I GTSAC 1 cut(s) 644
TspDTI ATGAA 4 cut(s) 137, 184, 399, 503
TspGWI ACGGA 2 cut(s) 439, 632
TspRI CASTG 2 cut(s) 292, 750
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 2 cut(s) 414, 590
XceI RCATGY 2 cut(s) 584, 652
XmiI GTMKAC 1 cut(s) 552
XmnI GAANNNNTTC 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.