pycom08g06070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015119
Physical Location & Seq
Forward (+)
4639056 .. 4641083
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g06070.2

Sequence Viewer

Length: 888 bp
ATGAAGTGGAATTCCGAAAATGCCCACTGTTTTCACAAAGCTTTTTTCTTTGCTTTCGTTTGGAAAGTTCAGGCGCTCGGAGGTCTGAGAGAGATGGAGGGAATACTAGAGGGGGGCGGATCGGGTGCGGGCAGATACACGCTGCGGCCGGAAAGGCTGAGCAACGAGGACATACTGTTCTGCATAGACGTTGACCCAGAGTCGATGGTGGAGATGAAGGCGGCGATGGGCCCGAACGGGCGACCCTTAACCCGATTGGACTCGATCAAGCAAGCCATACTTCTCTTCATCAACGCCAAGCTTACCATCAATCCGGAACACCGATTTGCATTCGCCACTCTCGGAAAGGCCGCGTCTTGGCTTCGAAGAGAGTTTAGCAGTGAAGTTGAGTCTGCTCATGCTGCACTTCGGATACTCTCAGCCATTCCTTCTTCTGGTAATGCAGATCTTACCGGTCTATTTCGAATAGCATCTCATGAAGCAAAGAAATCTCGCACTCAAAACAGAATTTTGAGGGTGGTTCTGATCTACTGTAGATCGTCCGTACGACCGCAAAATAATTGGCCGGTAAACCAGAAACTCTTCACCCTGGATGTTGTTTACCTTCATGACAAGCCTGGACCTGACAACTGCCCCCAGGAGGTCTACGATGCACTTGTGGATTCCCTGGAACATGTTAGCGAATTCGAGGGATACATCTTAGAGAGTGGCCAGGGATTAACACGAGTCCTCTTCCGTCACATGTGTGTGCTGCTATCGCACCCTCAGCAGCGATGCCCGCAAGACCACCTGGACATACCTAAGGCTCTCTCAAGACGGTTGCCGGCAGCAGACACAGTGAACGGTGAAGATAGTGTAACTGTATCCGGGGGCAAATCTGATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
No domains found.

Protein Analysis

296

Amino Acids

32.94

Weight (kDa)

7.17

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015119)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32960
fragaria_vesca FvH4_2g32160 FvH4_2g32160
malus_domestica MD08G1073300.v1.1
prunus_persica Prupe.1G414700_v2.0.a1 Prupe.6G150100_v2.0.a1
pyrus_communis pycom08g06070
rosa_chinensis RchiOBHm_Chr6g0310521
rosa_laevigata RLG00000010476
rosa_roxburghii Rroxscaffold_7G00158460
rosa_rugosa Rorug06G0381200
rosa_samantha Rh6AG495100 Rh6BG504700 Rh6CG510000 Rh6DG496000
rosa_wichuraiana Rw6G043060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 645
AccII CGCG 1 cut(s) 353
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 7 cut(s) 117, 128, 145, 221, 351, 551, 779
AclWI GGATC 1 cut(s) 127
AcoI YGGCCR 3 cut(s) 146, 563, 709
AcsI RAATTY 3 cut(s) 10, 507, 683
AfaI GTAC 1 cut(s) 546
AfiI CCNNNNNNNGG 3 cut(s) 357, 434, 640
AflIII ACRYGT 2 cut(s) 673, 741
AgeI ACCGGT 1 cut(s) 452
AhdI GACNNNNNGTC 1 cut(s) 199
AjnI CCWGG 6 cut(s) 588, 616, 636, 666, 711, 789
AleI CACNNNNGTG 1 cut(s) 744
AloI GAACNNNNNNTCC 2 cut(s) 161, 193
AluBI AGCT 2 cut(s) 41, 301
AluI AGCT 2 cut(s) 41, 301
AlwI GGATC 1 cut(s) 127
Aor13HI TCCGGA 1 cut(s) 313
AoxI GGCC 5 cut(s) 146, 229, 348, 563, 709
ApaI GGGCCC 1 cut(s) 233
ApeKI GCWGC 5 cut(s) 142, 401, 751, 769, 827
ApoI RAATTY 3 cut(s) 10, 507, 683
AsiGI ACCGGT 1 cut(s) 452
Asp700I GAANNNNTTC 1 cut(s) 581
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 3 cut(s) 229, 230, 620
AsuC2I CCSGG 1 cut(s) 868
AsuHPI GGTGA 2 cut(s) 577, 857
AsuII TTCGAA 2 cut(s) 364, 463
AvaII GGWCC 1 cut(s) 620
AxyI CCTNAGG 1 cut(s) 801
BaeGI GKGCMC 1 cut(s) 233
BalI TGGCCA 1 cut(s) 711
BanII GRGCYC 1 cut(s) 233
BauI CACGAG 1 cut(s) 723
BbvCI CCTCAGC 1 cut(s) 765
BbvI GCAGC 5 cut(s) 129, 388, 738, 781, 839
BccI CCATC 4 cut(s) 88, 199, 220, 314
BciT130I CCWGG 6 cut(s) 590, 618, 638, 668, 713, 791
BciVI GTATCC 3 cut(s) 405, 686, 874
BclI TGATCA 1 cut(s) 880
BcnI CCSGG 1 cut(s) 868
BfaI CTAG 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 532
BfoI RGCGCY 1 cut(s) 77
BfuI GTATCC 3 cut(s) 405, 686, 874
BglI GCCNNNNNGGC 1 cut(s) 154
BglII AGATCT 1 cut(s) 445
BisI GCNGC 8 cut(s) 143, 146, 222, 351, 402, 752, 770, 828
BlpI GCTNAGC 1 cut(s) 158
BlsI GCNGC 8 cut(s) 144, 147, 223, 352, 403, 753, 771, 829
Bme1390I CCNGG 7 cut(s) 590, 618, 638, 668, 713, 791, 868
Bme18I GGWCC 1 cut(s) 620
BmeRI GACNNNNNGTC 1 cut(s) 199
BmgT120I GGNCC 3 cut(s) 229, 230, 620
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 7 cut(s) 590, 618, 638, 668, 713, 791, 868
BmsI GCATC 3 cut(s) 479, 640, 764
Bpu10I CCTNAGC 1 cut(s) 765
Bpu1102I GCTNAGC 1 cut(s) 158
Bpu14I TTCGAA 2 cut(s) 364, 463
BpuEI CTTGAG 1 cut(s) 796
BpuMI CCSGG 1 cut(s) 868
BsaJI CCNNGG 5 cut(s) 588, 636, 666, 712, 867
BsaWI WCCGGW 2 cut(s) 313, 452
Bsc4I CCNNNNNNNGG 3 cut(s) 357, 434, 640
Bse118I RCCGGY 3 cut(s) 452, 565, 823
Bse21I CCTNAGG 1 cut(s) 801
BseAI TCCGGA 1 cut(s) 313
BseBI CCWGG 6 cut(s) 590, 618, 638, 668, 713, 791
BseDI CCNNGG 5 cut(s) 588, 636, 666, 712, 867
BseGI GGATG 1 cut(s) 598
BseLI CCNNNNNNNGG 3 cut(s) 357, 434, 640
BseMII CTCAG 4 cut(s) 77, 149, 432, 779
BseSI GKGCMC 1 cut(s) 233
BseX3I CGGCCG 1 cut(s) 146
BseXI GCAGC 5 cut(s) 129, 388, 738, 781, 839
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 353
Bsh1285I CGRYCG 2 cut(s) 149, 551
BshFI GGCC 5 cut(s) 148, 231, 350, 565, 711
BshTI ACCGGT 1 cut(s) 452
BsiEI CGRYCG 2 cut(s) 149, 551
BsiSI CCGG 6 cut(s) 149, 314, 453, 566, 824, 867
BsiWI CGTACG 1 cut(s) 544
BslI CCNNNNNNNGG 3 cut(s) 357, 434, 640
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 5 cut(s) 148, 231, 350, 565, 711
Bsp119I TTCGAA 2 cut(s) 364, 463
Bsp120I GGGCCC 1 cut(s) 229
Bsp1286I GDGCHC 1 cut(s) 233
Bsp13I TCCGGA 1 cut(s) 313
Bsp143I GATC 6 cut(s) 119, 264, 445, 525, 536, 880
Bsp1720I GCTNAGC 1 cut(s) 158
BspACI CCGC 7 cut(s) 117, 128, 145, 221, 351, 551, 779
BspANI GGCC 5 cut(s) 148, 231, 350, 565, 711
BspCNI CTCAG 4 cut(s) 78, 150, 431, 778
BspEI TCCGGA 1 cut(s) 313
BspFNI CGCG 1 cut(s) 353
BspHI TCATGA 2 cut(s) 475, 607
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 1 cut(s) 127
BspT104I TTCGAA 2 cut(s) 364, 463
BsrFI RCCGGY 3 cut(s) 452, 565, 823
BssAI RCCGGY 3 cut(s) 452, 565, 823
BssECI CCNNGG 5 cut(s) 588, 636, 666, 712, 867
BssMI GATC 6 cut(s) 119, 264, 445, 525, 536, 880
BssSI CACGAG 1 cut(s) 723
Bst2BI CACGAG 1 cut(s) 723
Bst2UI CCWGG 6 cut(s) 590, 618, 638, 668, 713, 791
Bst4CI ACNGT 7 cut(s) 29, 177, 533, 819, 838, 845, 862
Bst6I CTCTTC 4 cut(s) 290, 361, 587, 737
BstBI TTCGAA 2 cut(s) 364, 463
BstC8I GCNNGC 4 cut(s) 130, 273, 779, 825
BstDEI CTNAG 6 cut(s) 86, 158, 418, 700, 765, 801
BstF5I GGATG 1 cut(s) 598
BstFNI CGCG 1 cut(s) 353
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 6 cut(s) 122, 267, 448, 528, 539, 883
BstMBI GATC 6 cut(s) 119, 264, 445, 525, 536, 880
BstMCI CGRYCG 2 cut(s) 149, 551
BstMWI GCNNNNNNNGC 5 cut(s) 154, 401, 757, 766, 778
BstNI CCWGG 6 cut(s) 590, 618, 638, 668, 713, 791
BstNSI RCATGY 2 cut(s) 677, 745
BstSCI CCNGG 7 cut(s) 588, 616, 636, 666, 711, 789, 866
BstSFI CTRYAG 1 cut(s) 532
BstSLI GKGCMC 1 cut(s) 233
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 5 cut(s) 129, 388, 738, 781, 839
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
BstZI CGGCCG 1 cut(s) 146
Bsu36I CCTNAGG 1 cut(s) 801
BsuI GTATCC 3 cut(s) 405, 686, 874
BsuRI GGCC 5 cut(s) 148, 231, 350, 565, 711
BtgZI GCGATG 2 cut(s) 239, 787
BtsCI GGATG 1 cut(s) 598
BtsI GCAGTG 1 cut(s) 385
BtsIMutI CAGTG 3 cut(s) 25, 385, 843
Cac8I GCNNGC 4 cut(s) 130, 273, 779, 825
CciI TCATGA 2 cut(s) 475, 607
CfoI GCGC 1 cut(s) 76
Cfr10I RCCGGY 3 cut(s) 452, 565, 823
Cfr13I GGNCC 3 cut(s) 229, 230, 620
CseI GACGC 1 cut(s) 342
Csp6I GTAC 1 cut(s) 545
CspAI ACCGGT 1 cut(s) 452
CviAII CATG 5 cut(s) 398, 476, 608, 674, 742
CviQI GTAC 1 cut(s) 545
DdeI CTNAG 6 cut(s) 86, 158, 418, 700, 765, 801
DpnI GATC 6 cut(s) 121, 266, 447, 527, 538, 882
DpnII GATC 6 cut(s) 119, 264, 445, 525, 536, 880
DriI GACNNNNNGTC 1 cut(s) 199
EaeI YGGCCR 3 cut(s) 146, 563, 709
EagI CGGCCG 1 cut(s) 146
Eam1104I CTCTTC 4 cut(s) 290, 361, 587, 737
Eam1105I GACNNNNNGTC 1 cut(s) 199
EarI CTCTTC 4 cut(s) 290, 361, 587, 737
EciI GGCGGA 1 cut(s) 132
EclXI CGGCCG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 233
Eco47I GGWCC 1 cut(s) 620
Eco52I CGGCCG 1 cut(s) 146
Eco81I CCTNAGG 1 cut(s) 801
EcoRI GAATTC 2 cut(s) 10, 683
EcoRII CCWGG 6 cut(s) 588, 616, 636, 666, 711, 789
EcoT38I GRGCYC 1 cut(s) 233
FaeI CATG 5 cut(s) 401, 479, 611, 677, 745
FaiI YATR 9 cut(s) 173, 185, 278, 399, 477, 609, 675, 743, 797
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FatI CATG 5 cut(s) 397, 475, 607, 673, 741
FauI CCCGC 2 cut(s) 121, 786
FbaI TGATCA 1 cut(s) 880
FblI GTMKAC 1 cut(s) 645
Fnu4HI GCNGC 8 cut(s) 143, 146, 222, 351, 402, 752, 770, 828
FokI GGATG 1 cut(s) 605
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 8 cut(s) 143, 146, 222, 351, 402, 752, 770, 828
FspBI CTAG 1 cut(s) 107
GlaI GCGC 1 cut(s) 75
GluI GCNGC 8 cut(s) 143, 146, 222, 351, 402, 752, 770, 828
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 5 cut(s) 148, 231, 350, 565, 711
HapII CCGG 6 cut(s) 149, 314, 453, 566, 824, 867
HgaI GACGC 1 cut(s) 342
HhaI GCGC 1 cut(s) 76
Hin1II CATG 5 cut(s) 401, 479, 611, 677, 745
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 2 cut(s) 39, 299
HinfI GANTC 5 cut(s) 200, 260, 389, 662, 726
HpaII CCGG 6 cut(s) 149, 314, 453, 566, 824, 867
HphI GGTGA 2 cut(s) 577, 857
Hpy166II GTNNAC 5 cut(s) 193, 571, 601, 646, 841
Hpy188I TCNGA 7 cut(s) 16, 80, 87, 344, 411, 525, 880
Hpy188III TCNNGA 4 cut(s) 314, 476, 608, 813
Hpy8I GTNNAC 5 cut(s) 193, 571, 601, 646, 841
HpyAV CCTTC 3 cut(s) 211, 438, 614
HpyCH4III ACNGT 7 cut(s) 29, 177, 533, 819, 838, 845, 862
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 5 cut(s) 183, 329, 404, 443, 653
HpyF10VI GCNNNNNNNGC 5 cut(s) 154, 401, 757, 766, 778
HpyF3I CTNAG 6 cut(s) 86, 158, 418, 700, 765, 801
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 5 cut(s) 401, 479, 611, 677, 745
HspAI GCGC 1 cut(s) 74
Kpn2I TCCGGA 1 cut(s) 313
KroI GCCGGC 1 cut(s) 823
KroNI GCCGGC 1 cut(s) 825
Ksp22I TGATCA 1 cut(s) 880
Kzo9I GATC 6 cut(s) 119, 264, 445, 525, 536, 880
Lsp1109I GCAGC 5 cut(s) 129, 388, 738, 781, 839
LweI GCATC 3 cut(s) 479, 640, 764
MaeI CTAG 1 cut(s) 107
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 2 cut(s) 737, 856
MalI GATC 6 cut(s) 121, 266, 447, 527, 538, 882
MboI GATC 6 cut(s) 119, 264, 445, 525, 536, 880
MboII GAAGA 6 cut(s) 277, 378, 423, 574, 724, 860
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 711
MluCI AATT 4 cut(s) 10, 507, 559, 683
MluNI TGGCCA 1 cut(s) 711
MlyI GAGTC 4 cut(s) 209, 254, 398, 735
MnlI CCTC 9 cut(s) 74, 91, 103, 160, 507, 634, 682, 740, 774
Mox20I TGGCCA 1 cut(s) 711
MroI TCCGGA 1 cut(s) 313
MroNI GCCGGC 1 cut(s) 823
MroXI GAANNNNTTC 1 cut(s) 581
MscI TGGCCA 1 cut(s) 711
MseI TTAA 2 cut(s) 248, 719
MslI CAYNNNNRTG 2 cut(s) 744, 746
Msp20I TGGCCA 1 cut(s) 711
MspI CCGG 6 cut(s) 149, 314, 453, 566, 824, 867
MspR9I CCNGG 7 cut(s) 590, 618, 638, 668, 713, 791, 868
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 6 cut(s) 590, 618, 638, 668, 713, 791
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 5 cut(s) 154, 401, 757, 766, 778
NaeI GCCGGC 1 cut(s) 825
NciI CCSGG 1 cut(s) 868
NdeII GATC 6 cut(s) 119, 264, 445, 525, 536, 880
NgoMIV GCCGGC 1 cut(s) 823
NlaIII CATG 5 cut(s) 401, 479, 611, 677, 745
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 737
NspI RCATGY 2 cut(s) 677, 745
NspV TTCGAA 2 cut(s) 364, 463
OliI CACNNNNGTG 1 cut(s) 744
PagI TCATGA 2 cut(s) 475, 607
PciI ACATGT 2 cut(s) 673, 741
PctI GAATGC 1 cut(s) 329
PdiI GCCGGC 1 cut(s) 825
PdmI GAANNNNTTC 1 cut(s) 581
PfeI GAWTC 1 cut(s) 662
Pfl23II CGTACG 1 cut(s) 544
PinAI ACCGGT 1 cut(s) 452
PkrI GCNGC 8 cut(s) 144, 147, 223, 352, 403, 753, 771, 829
PleI GAGTC 4 cut(s) 208, 254, 397, 734
PpsI GAGTC 4 cut(s) 208, 254, 397, 734
PscI ACATGT 2 cut(s) 673, 741
Psp6I CCWGG 6 cut(s) 588, 616, 636, 666, 711, 789
PspGI CCWGG 6 cut(s) 588, 616, 636, 666, 711, 789
PspLI CGTACG 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 231
PspOMI GGGCCC 1 cut(s) 229
PspPI GGNCC 3 cut(s) 229, 230, 620
PsuI RGATCY 1 cut(s) 445
RsaI GTAC 1 cut(s) 546
RsaNI GTAC 1 cut(s) 545
RseI CAYNNNNRTG 2 cut(s) 744, 746
SaqAI TTAA 2 cut(s) 248, 719
SatI GCNGC 8 cut(s) 143, 146, 222, 351, 402, 752, 770, 828
Sau3AI GATC 6 cut(s) 119, 264, 445, 525, 536, 880
Sau96I GGNCC 3 cut(s) 229, 230, 620
SchI GAGTC 4 cut(s) 209, 254, 398, 735
ScrFI CCNGG 7 cut(s) 590, 618, 638, 668, 713, 791, 868
SduI GDGCHC 1 cut(s) 233
SetI ASST 9 cut(s) 43, 85, 192, 303, 606, 625, 645, 792, 802
SfaNI GCATC 3 cut(s) 479, 640, 764
SfcI CTRYAG 1 cut(s) 532
SfuI TTCGAA 2 cut(s) 364, 463
SinI GGWCC 1 cut(s) 620
SmiMI CAYNNNNRTG 2 cut(s) 744, 746
SmlI CTYRAG 1 cut(s) 811
SmoI CTYRAG 1 cut(s) 811
Sse9I AATT 4 cut(s) 10, 507, 559, 683
SsiI CCGC 7 cut(s) 117, 128, 145, 221, 351, 551, 779
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 7 cut(s) 588, 616, 636, 666, 711, 789, 866
TaaI ACNGT 7 cut(s) 29, 177, 533, 819, 838, 845, 862
TaiI ACGT 1 cut(s) 192
TaqI TCGA 5 cut(s) 203, 263, 364, 463, 687
TasI AATT 4 cut(s) 10, 507, 559, 683
TauI GCSGC 3 cut(s) 148, 224, 353
TfiI GAWTC 1 cut(s) 662
Tru1I TTAA 2 cut(s) 248, 719
Tru9I TTAA 2 cut(s) 248, 719
TscAI CASTG 3 cut(s) 32, 385, 843
TseFI GTSAC 1 cut(s) 737
TseI GCWGC 5 cut(s) 142, 401, 751, 769, 827
Tsp45I GTSAC 1 cut(s) 737
TspDTI ATGAA 5 cut(s) 17, 230, 277, 492, 596
TspGWI ACGGA 2 cut(s) 532, 725
TspRI CASTG 3 cut(s) 32, 385, 843
VpaK11BI GGWCC 1 cut(s) 620
XapI RAATTY 3 cut(s) 10, 507, 683
XceI RCATGY 2 cut(s) 677, 745
XmiI GTMKAC 1 cut(s) 645
XmnI GAANNNNTTC 1 cut(s) 581
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.