Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0009577

Physical Location & Seq

Genomic Coordinates Forward (+)

24713782 .. 24724924

Gene Structure 11143 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g32680.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2850 bp

ATGGCGAATAATCCTCAGTCCTCAGCTGCTCAGTATCGACCAATGGTACCAGCACAGCAAGGACAGCACTTCATATCACCAGGTTCCCAACAATTTCAGCCTGTGGGACAAGGTCAGCCATTGCAATATTCTCAACAAATGCAGCCGTATCCTCTTAGGCCAAATCAGCCTGGTCATGCTCAACCTTCATCACAGGCATTACCTATGCCATACTATCAGCCGAGGCCAGTTACATCTGTCCCACCACACTCTCAGCAACCTGCTCCACCTTTCAATAATCAGATGCCTGGAATGCCCTATCCTTCATCATATATGTATGCACAGCCTTCTTATGCCCAGCCACAGAATAATGCCAATTCTTCATCACAGTTTCAGCCAATGTCTCAAGATCAAGCACATGGTGTACCTACTGCTGGACAACCTTGGATGTCCTCCAGTAGTCATCAGGGGGCAGCAGTTACACCACAACAGCAGCCCAGCCAGCAACCTACAAGCACTCCTTTCCCAGATCCTGCAGTAAATGCTCCTAATCTGGCACAGCCATCCTCTTCTGATTGGCAAGAGCATATGGCGTCTGATGGGAGAAGATATTACTTCAATAGAAGTACTAGACAATCTAGTTGGGAGAAACCTTTAGAATTGATGACTCCACTGGAGAGAGCCGATGCATCAACTGTTTGGAAAGAGTACACTTCTGCAGATGGGAAAAAGTATTACTACAACAAGGTTACAAGGGAGTCTAAATGGACAATTCCAGAGGAGTTAAAGCTGGCTCGTGAGCAAGCTCAAAGAGAACATACTCAGGGTACACAATCAGAGATGACTTCAACTTCTCATGCCCCTCCTGCTACTGCTTCAGCTGAAATACATGCTGGAGCTAGTTCAGTTGGGCCCAGCACTTCTTCAGCCCAACCTGGGACAGTCTCGAGTCCAGTTGCAGTGACACCCATCTCTGCCTTCTCTAATCCTTCTCCTACTACGCCTTCGGGATTATCGGTTGCTCCTGGTGTACAATCTTCCATGGCAACGGGGTCTGTTGGAGTTCAACCTGCAGTGGTAAATGTCTCCCCACTTCCTGCTTCTAATGTGGGGAGTACCGGTCTTCCCTCTACTTTGGTCAACACCATTACAAAATCAGTTAATGAAAATCAGGCACCTCAAGATAGTGCAAGTTCAATTGATGGAGCTTCTAGTCAAGATATTGAGGAAGCGAAGAAGGGAATGGCAGTTGCTGGGAAAGTCAATGTGACTCCTTCAGAGGAGAAAGCAATTGATGATGAGCCATTGGTGTATGCCAGTAAGCAGGAGGCAAAAAATGCATTCAAATCACTCCTGGAATCTGCAAATGTACACTCTGATTGGACCTGGGAGCAGGCAATGAGAGAGATCATTAATGACAAAAGATATGGCGCTTTAAGAACACTAGGTGAGCGGAAGCAAGCATTTAATGAGTACTTGGGCCAAAGGAAAAAGCTGGAGAATGAAGAGAGGCGCATAAGGCAGAAAAGGGCTCGGGAAGAATTTACAAAAATGCTGGAAGAGTCCAAGGAACTGACATCAACAATTAGATGGAGCAAAGCTGTAACTATGTTTGAAAATGATGAACGATTCAAGGCTGTTGAACGAGCTAGAGATCGGGAAGATCTCTATGAGAGCTACATTGTAGAACTTGAGAGGAAGGAGAAAGAGATTGCTGCTGAAGAGCATAGGAGGAATATATCCGAGTACAAGGAATTTCTGGAGTCTTGCGACTTTATTAAGGTGAACACCCAGTGGCGTAAAGTTCAAGATCGCTTAGAGGACGATGAGAGATGCTTGCGTCTTGATAAGTTTGACCGCTTGCTTATTTTCCAGGACCATATTCGTGACCTTGAAAAGGAGGAAGAGGAGCAAAAGAAGATACAAAAGGAACAATTGAGGCGGATTGAGAGGAAAAACCGGGATGAGTTTCGCAAGATTTTGGAAGAACATGCTGCTGACGGTACTCTTACGGCTAAAACTCAGTGGCGCGATTACTGTATGAAGGTCAAAGACTTGCCACAGTACGAAGCTGTTGCAGCAAACACACATGGTTCTTCAACACCAAAAGATTTGTTTGAGGATGTTGCTGAAGATTTAGAGAAACAGTTTGTGGAAGATAAAGCACGGGTAAAGGATGCAATGAAGCAAGGGCAGATTACAATGGTTTCGTCATGGACATTTGAAGAATTTAAGGCTGCTGTTGTGAATGATATTGGTTTCCCATCAATTTCCGAGCTTAATTTGAAGCTTGCTTACGAGGATATATTAGAGAGAGCCAGGGAGAAGGAGGAGAAAGAAGCAAAGAAGCGGCTACGAATTGCTGATGACTTCCATAAGCTTTTGCACACGTTCAAGGAAATAACTGTTTCTTCTAGCTGGGAAGATTGCAAACAACTTTTTGAAGAGACCCAAGAATACAGATCAGTTGGGGATGAGGACTTTGGAAGGGAAATTTTTGAGGAATATATTACAAGTTTACACGAGAGGGCCAAAGAAAAAGAACGGAAGCGTGAGGAGGAAAAGGCAAAAAAGGAGAAGGAAAGAGAGGAGAAAGAGAAGCGGAAGGATAAGGAGAGAAAGGAGAAGGATAGAGAACGTGAAAAAGAGAAGGGAAAGGAAAGATCTAAGAAGGATGAAACAGATAGTGAAACTGTTGACATGACTGATAGTCATGATCACAAAGAAGATAAGAAAAGGGAGAAAGACAAAGATAGGAAGCACCGGAAGCGGCATCAAAGTTCCATTGATGACGTGGGTTCTGATAAGGAAGAGAAAGAGGACTCTAAGAAACACAGACATAGTAGTGACCGCAAAAAATCAAGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005685 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006928 GO:0006996 GO:0007010 GO:0008150 GO:0008152 GO:0008360 GO:0008380 GO:0009987 GO:0010467 GO:0010564 GO:0016043 GO:0016070 GO:0016071 GO:0016363 GO:0016477 GO:0016592 GO:0016604 GO:0016607 GO:0022603 GO:0022604 GO:0030532 GO:0031974 GO:0031981 GO:0032465 GO:0032991 GO:0034399 GO:0034641 GO:0040011 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051302 GO:0051674 GO:0051726 GO:0065007 GO:0065008 GO:0070013 GO:0070064 GO:0071004 GO:0071010 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097525 GO:0120114 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

FF WW

Protein Analysis

950

Amino Acids

108.62

Weight (kDa)

6.18

Isoelectric Point (pI)

59.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	184 - 211	9.1e-10	WW domain
WW	PF00397	227 - 252	1.2e-07	WW domain
FF	PF01846	436 - 485	6.3e-17	FF domain
FF	PF01846	503 - 553	8.1e-13	FF domain
FF	PF01846	645 - 701	3.7e-06	FF domain
FF_PRPF40A	PF25432	709 - 768	1.7e-19	PRPF40A-like, FF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44910 AT1G44910
fragaria_vesca	FvH4_2g32680 FvH4_2g32680 FvH4_2g32680 FvH4_2g32680
malus_domestica	MD08G1078500.v1.1 MD15G1065300.v1.1
prunus_persica	Prupe.1G419400_v2.0.a1
pyrus_communis	pycom08g06490 pycom15g06180
rosa_chinensis	RchiOBHm_Chr6g0309991
rosa_laevigata	RLG00000010518
rosa_multiflora	Rmu_sc0000294.1_g000011
rosa_roxburghii	Rroxscaffold_7G00158970
rosa_rugosa	Rorug06G0376700 Rorug06G0376800
rosa_samantha	Rh6AG490400 Rh6BG499900 Rh6CG504800 Rh6DG490800
rosa_wichuraiana	Rw6G042690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	268, 1057
Acc65I	GGTACC	1 cut(s)	46
AccB1I	GGYRCC	2 cut(s)	46, 1153
AccBSI	CCGCTC	1 cut(s)	1432
AccII	CGCG	1 cut(s)	2010
AciI	CCGC	7 cut(s)	1432, 1837, 1921, 2329, 2581, 2749, 2830
AclWI	GGATC	1 cut(s)	503
AcsI	RAATTY	4 cut(s)	1520, 1733, 2207, 2472
AcuI	CTGAAG	5 cut(s)	840, 888, 1239, 1719, 2130
AcyI	GRCGYC	1 cut(s)	572
AdeI	CACNNNGTG	3 cut(s)	401, 1427, 1773
AfiI	CCNNNNNNNGG	3 cut(s)	413, 915, 1876
AflIII	ACRYGT	1 cut(s)	2367
AgeI	ACCGGT	1 cut(s)	1097
AhdI	GACNNNNNGTC	1 cut(s)	2688
AjiI	CACGTC	1 cut(s)	2773
AjnI	CCWGG	9 cut(s)	79, 169, 286, 913, 1003, 1332, 1364, 1851, 2297
Alw26I	GTCTC	4 cut(s)	387, 928, 1069, 2420
AlwI	GGATC	1 cut(s)	503
AlwNI	CAGNNNCTG	2 cut(s)	512, 1232
Ama87I	CYCGRG	2 cut(s)	925, 1512
AoxI	GGCC	5 cut(s)	158, 224, 890, 1459, 2508
ApaI	GGGCCC	1 cut(s)	894
ApeKI	GCWGC	8 cut(s)	26, 142, 452, 472, 1694, 1973, 2057, 2216
ApoI	RAATTY	4 cut(s)	1520, 1733, 2207, 2472
AseI	ATTAAT	1 cut(s)	1392
AsiGI	ACCGGT	1 cut(s)	1097
Asp718I	GGTACC	1 cut(s)	46
AspLEI	GCGC	3 cut(s)	1412, 1494, 2010
AspS9I	GGNCC	6 cut(s)	890, 891, 1362, 1459, 1855, 2508
AsuC2I	CCSGG	1 cut(s)	1940
AsuHPI	GGTGA	3 cut(s)	69, 1439, 1774
AvaI	CYCGRG	2 cut(s)	925, 1512
AvaII	GGWCC	2 cut(s)	1362, 1855
BaeGI	GKGCMC	1 cut(s)	894
BanI	GGYRCC	2 cut(s)	46, 1153
BanII	GRGCYC	2 cut(s)	894, 1513
BarI	GAAGNNNNNNTAC	2 cut(s)	2258, 2290
BauI	CACGAG	2 cut(s)	774, 2501
BbsI	GAAGAC	1 cut(s)	1094
BbvCI	CCTCAGC	1 cut(s)	22
BbvI	GCAGC	8 cut(s)	13, 154, 464, 484, 1681, 1960, 2069, 2203
BccI	CCATC	7 cut(s)	550, 572, 695, 956, 1175, 1563, 2251
BceAI	ACGGC	2 cut(s)	130, 2007
BcgI	CGANNNNNNTGC	2 cut(s)	2036, 2070
BciT130I	CCWGG	9 cut(s)	81, 171, 288, 915, 1005, 1334, 1366, 1853, 2299
BciVI	GTATCC	1 cut(s)	159
BclI	TGATCA	1 cut(s)	2695
BcnI	CCSGG	1 cut(s)	1940
BcoDI	GTCTC	4 cut(s)	387, 928, 1069, 2420
BfaI	CTAG	7 cut(s)	609, 618, 879, 1191, 1424, 1629, 2394
BfmI	CTRYAG	3 cut(s)	513, 696, 1050
BfoI	RGCGCY	1 cut(s)	1413
BfuAI	ACCTGC	2 cut(s)	268, 1057
BfuI	GTATCC	1 cut(s)	159
BglII	AGATCT	2 cut(s)	1642, 2642
BmcAI	AGTACT	2 cut(s)	607, 1454
Bme18I	GGWCC	2 cut(s)	1362, 1855
BmeRI	GACNNNNNGTC	1 cut(s)	2688
BmeT110I	CYCGRG	2 cut(s)	925, 1512
BmgBI	CACGTC	1 cut(s)	2773
BmgT120I	GGNCC	6 cut(s)	890, 891, 1362, 1459, 1855, 2508
BmiI	GGNNCC	4 cut(s)	48, 85, 892, 1155
BmrI	ACTGGG	1 cut(s)	1765
BmsI	GCATC	6 cut(s)	273, 655, 677, 1802, 2146, 2761
BmuI	ACTGGG	1 cut(s)	1765
BpiI	GAAGAC	1 cut(s)	1094
BpmI	CTGGAG	5 cut(s)	418, 674, 894, 1496, 1760
Bpu10I	CCTNAGC	1 cut(s)	22
BpuEI	CTTGAG	3 cut(s)	369, 1143, 1691
BpuMI	CCSGG	1 cut(s)	1940
BsaHI	GRCGYC	1 cut(s)	572
BsaI	GGTCTC	1 cut(s)	2420
BsaJI	CCNNGG	7 cut(s)	221, 422, 914, 1020, 1365, 1545, 2298
BsaWI	WCCGGW	2 cut(s)	1097, 2742
BsaXI	ACNNNNNCTCC	4 cut(s)	481, 511, 1084, 1114
Bsc4I	CCNNNNNNNGG	3 cut(s)	413, 915, 1876
Bse118I	RCCGGY	1 cut(s)	1097
Bse1I	ACTGG	6 cut(s)	227, 435, 657, 932, 1296, 1771
Bse3DI	GCAATG	3 cut(s)	119, 1383, 2166
BseBI	CCWGG	9 cut(s)	81, 171, 288, 915, 1005, 1334, 1366, 1853, 2299
BseDI	CCNNGG	7 cut(s)	221, 422, 914, 1020, 1365, 1545, 2298
BseGI	GGATG	7 cut(s)	432, 542, 1948, 2107, 2161, 2458, 2659
BseLI	CCNNNNNNNGG	3 cut(s)	413, 915, 1876
BseMI	GCAATG	3 cut(s)	119, 1383, 2166
BseMII	CTCAG	6 cut(s)	29, 36, 44, 266, 815, 2015
BseNI	ACTGG	6 cut(s)	227, 435, 657, 932, 1296, 1771
BseRI	GAGGAG	6 cut(s)	773, 1274, 1901, 2324, 2549, 2582
BseSI	GKGCMC	1 cut(s)	894
BseXI	GCAGC	8 cut(s)	13, 154, 464, 484, 1681, 1960, 2069, 2203
BseYI	CCCAGC	5 cut(s)	336, 476, 893, 1232, 2397
Bsh1236I	CGCG	1 cut(s)	2010
BshFI	GGCC	5 cut(s)	160, 226, 892, 1461, 2510
BshNI	GGYRCC	2 cut(s)	46, 1153
BshTI	ACCGGT	1 cut(s)	1097
BsiHKCI	CYCGRG	2 cut(s)	925, 1512
BsiSI	CCGG	3 cut(s)	1098, 1939, 2743
BslFI	GGGAC	3 cut(s)	120, 224, 931
BslI	CCNNNNNNNGG	3 cut(s)	413, 915, 1876
BsmAI	GTCTC	4 cut(s)	387, 928, 1069, 2420
BsmFI	GGGAC	3 cut(s)	120, 224, 931
BsmI	GAATGC	2 cut(s)	297, 1319
BsnI	GGCC	5 cut(s)	160, 226, 892, 1461, 2510
Bso31I	GGTCTC	1 cut(s)	2420
BsoBI	CYCGRG	2 cut(s)	925, 1512
Bsp120I	GGGCCC	1 cut(s)	890
Bsp1286I	GDGCHC	2 cut(s)	894, 1513
Bsp1407I	TGTACA	2 cut(s)	1009, 1348
Bsp143I	GATC	9 cut(s)	388, 508, 1386, 1633, 1642, 1789, 2441, 2642, 2695
Bsp19I	CCATGG	1 cut(s)	1020
BspACI	CCGC	7 cut(s)	1432, 1837, 1921, 2329, 2581, 2749, 2830
BspANI	GGCC	5 cut(s)	160, 226, 892, 1461, 2510
BspCNI	CTCAG	6 cut(s)	28, 35, 43, 265, 814, 2014
BspFNI	CGCG	1 cut(s)	2010
BspHI	TCATGA	1 cut(s)	2692
BspLI	GGNNCC	4 cut(s)	48, 85, 892, 1155
BspMAI	CTGCAG	3 cut(s)	517, 700, 1054
BspMI	ACCTGC	2 cut(s)	268, 1057
BspPI	GGATC	1 cut(s)	503
BspQI	GCTCTTC	1 cut(s)	1695
BspT107I	GGYRCC	2 cut(s)	46, 1153
BspTNI	GGTCTC	1 cut(s)	2420
BsrBI	CCGCTC	1 cut(s)	1432
BsrDI	GCAATG	3 cut(s)	119, 1383, 2166
BsrFI	RCCGGY	1 cut(s)	1097
BsrGI	TGTACA	2 cut(s)	1009, 1348
BsrI	ACTGG	6 cut(s)	227, 435, 657, 932, 1296, 1771
BssAI	RCCGGY	1 cut(s)	1097
BssECI	CCNNGG	7 cut(s)	221, 422, 914, 1020, 1365, 1545, 2298
BssMI	GATC	9 cut(s)	388, 508, 1386, 1633, 1642, 1789, 2441, 2642, 2695
BssNI	GRCGYC	1 cut(s)	572
BssSI	CACGAG	2 cut(s)	774, 2501
BssT1I	CCWWGG	3 cut(s)	422, 1020, 1545
Bst2BI	CACGAG	2 cut(s)	774, 2501
Bst2UI	CCWGG	9 cut(s)	81, 171, 288, 915, 1005, 1334, 1366, 1853, 2299
Bst4CI	ACNGT	9 cut(s)	369, 676, 922, 1982, 2018, 2043, 2127, 2386, 2674
Bst6I	CTCTTC	7 cut(s)	553, 1479, 1533, 1695, 1878, 2418, 2784
BstACI	GRCGYC	1 cut(s)	572
BstAPI	GCANNNNNTGC	2 cut(s)	521, 1316
BstAUI	TGTACA	2 cut(s)	1009, 1348
BstC8I	GCNNGC	8 cut(s)	482, 771, 783, 1374, 1440, 1817, 1841, 2271
BstDSI	CCRYGG	1 cut(s)	1020
BstENI	CCTNNNNNAGG	1 cut(s)	1874
BstF5I	GGATG	7 cut(s)	432, 542, 1948, 2107, 2161, 2458, 2659
BstFNI	CGCG	1 cut(s)	2010
BstH2I	RGCGCY	1 cut(s)	1413
BstHHI	GCGC	3 cut(s)	1412, 1494, 2010
BstKTI	GATC	9 cut(s)	391, 511, 1389, 1636, 1645, 1792, 2444, 2645, 2698
BstMAI	GTCTC	4 cut(s)	387, 928, 1069, 2420
BstMBI	GATC	9 cut(s)	388, 508, 1386, 1633, 1642, 1789, 2441, 2642, 2695
BstNI	CCWGG	9 cut(s)	81, 171, 288, 915, 1005, 1334, 1366, 1853, 2299
BstNSI	RCATGY	2 cut(s)	872, 1973
BstSFI	CTRYAG	3 cut(s)	513, 696, 1050
BstSLI	GKGCMC	1 cut(s)	894
BstUI	CGCG	1 cut(s)	2010
BstV1I	GCAGC	8 cut(s)	13, 154, 464, 484, 1681, 1960, 2069, 2203
BstV2I	GAAGAC	1 cut(s)	1094
BstX2I	RGATCY	3 cut(s)	508, 1642, 2642
BstYI	RGATCY	3 cut(s)	508, 1642, 2642
BsuI	GTATCC	1 cut(s)	159
BsuRI	GGCC	5 cut(s)	160, 226, 892, 1461, 2510
BtgI	CCRYGG	1 cut(s)	1020
BtrI	CACGTC	1 cut(s)	2773
BtsCI	GGATG	7 cut(s)	432, 542, 1948, 2107, 2161, 2458, 2659
BtsI	GCAGTG	2 cut(s)	945, 1059
BtsIMutI	CAGTG	5 cut(s)	650, 945, 1059, 1778, 2009
BveI	ACCTGC	2 cut(s)	268, 1057
Cac8I	GCNNGC	8 cut(s)	482, 771, 783, 1374, 1440, 1817, 1841, 2271
CaiI	CAGNNNCTG	2 cut(s)	512, 1232
CciI	TCATGA	1 cut(s)	2692
CfoI	GCGC	3 cut(s)	1412, 1494, 2010
Cfr10I	RCCGGY	1 cut(s)	1097
Cfr13I	GGNCC	6 cut(s)	890, 891, 1362, 1459, 1855, 2508
CseI	GACGC	2 cut(s)	561, 1808
CsiI	ACCWGGT	1 cut(s)	79
CspAI	ACCGGT	1 cut(s)	1097
DpnI	GATC	9 cut(s)	390, 510, 1388, 1635, 1644, 1791, 2443, 2644, 2697
DpnII	GATC	9 cut(s)	388, 508, 1386, 1633, 1642, 1789, 2441, 2642, 2695
DraIII	CACNNNGTG	3 cut(s)	401, 1427, 1773
DriI	GACNNNNNGTC	1 cut(s)	2688
Eam1104I	CTCTTC	7 cut(s)	553, 1479, 1533, 1695, 1878, 2418, 2784
Eam1105I	GACNNNNNGTC	1 cut(s)	2688
EarI	CTCTTC	7 cut(s)	553, 1479, 1533, 1695, 1878, 2418, 2784
EciI	GGCGGA	1 cut(s)	1936
Eco130I	CCWWGG	3 cut(s)	422, 1020, 1545
Eco24I	GRGCYC	2 cut(s)	894, 1513
Eco31I	GGTCTC	1 cut(s)	2420
Eco47I	GGWCC	2 cut(s)	1362, 1855
Eco57I	CTGAAG	5 cut(s)	840, 888, 1239, 1719, 2130
Eco88I	CYCGRG	2 cut(s)	925, 1512
EcoNI	CCTNNNNNAGG	1 cut(s)	1874
EcoRII	CCWGG	9 cut(s)	79, 169, 286, 913, 1003, 1332, 1364, 1851, 2297
EcoT14I	CCWWGG	3 cut(s)	422, 1020, 1545
EcoT22I	ATGCAT	2 cut(s)	670, 1321
EcoT38I	GRGCYC	2 cut(s)	894, 1513
ErhI	CCWWGG	3 cut(s)	422, 1020, 1545
FalI	AAGNNNNNCTT	2 cut(s)	484, 516
FaqI	GGGAC	3 cut(s)	120, 224, 931
FauNDI	CATATG	1 cut(s)	567
FbaI	TGATCA	1 cut(s)	2695
FokI	GGATG	7 cut(s)	439, 529, 1955, 2114, 2168, 2465, 2666
FriOI	GRGCYC	2 cut(s)	894, 1513
FspBI	CTAG	7 cut(s)	609, 618, 879, 1191, 1424, 1629, 2394
GlaI	GCGC	3 cut(s)	1411, 1493, 2009
GsaI	CCCAGC	5 cut(s)	340, 480, 897, 1236, 2401
GsuI	CTGGAG	5 cut(s)	418, 674, 894, 1496, 1760
HaeII	RGCGCY	1 cut(s)	1413
HaeIII	GGCC	5 cut(s)	160, 226, 892, 1461, 2510
HapII	CCGG	3 cut(s)	1098, 1939, 2743
HgaI	GACGC	2 cut(s)	561, 1808
HhaI	GCGC	3 cut(s)	1412, 1494, 2010
Hin1I	GRCGYC	1 cut(s)	572
Hin6I	GCGC	3 cut(s)	1410, 1492, 2008
HinP1I	GCGC	3 cut(s)	1410, 1492, 2008
HincII	GTYRAC	2 cut(s)	1120, 2677
HindII	GTYRAC	2 cut(s)	1120, 2677
HindIII	AAGCTT	2 cut(s)	2267, 2357
HinfI	GANTC	9 cut(s)	646, 737, 928, 1249, 1337, 1541, 1608, 1742, 2801
HpaII	CCGG	3 cut(s)	1098, 1939, 2743
HphI	GGTGA	3 cut(s)	69, 1439, 1774
Hpy166II	GTNNAC	9 cut(s)	404, 690, 809, 1010, 1120, 1351, 1765, 2498, 2677
Hpy188I	TCNGA	9 cut(s)	282, 553, 577, 817, 1258, 1357, 1723, 2254, 2782
Hpy8I	GTNNAC	9 cut(s)	404, 690, 809, 1010, 1120, 1351, 1765, 2498, 2677
HpyCH4III	ACNGT	9 cut(s)	369, 676, 922, 1982, 2018, 2043, 2127, 2386, 2674
HpyCH4IV	ACGT	3 cut(s)	2369, 2617, 2772
HpySE526I	ACGT	3 cut(s)	2369, 2617, 2772
Hsp92I	GRCGYC	1 cut(s)	572
HspAI	GCGC	3 cut(s)	1410, 1492, 2008
KpnI	GGTACC	1 cut(s)	50
Ksp22I	TGATCA	1 cut(s)	2695
Kzo9I	GATC	9 cut(s)	388, 508, 1386, 1633, 1642, 1789, 2441, 2642, 2695
LguI	GCTCTTC	1 cut(s)	1695
LmnI	GCTCC	8 cut(s)	268, 529, 875, 1006, 1184, 1369, 1572, 1888
Lsp1109I	GCAGC	8 cut(s)	13, 154, 464, 484, 1681, 1960, 2069, 2203
LweI	GCATC	6 cut(s)	273, 655, 677, 1802, 2146, 2761
MabI	ACCWGGT	1 cut(s)	79
MaeI	CTAG	7 cut(s)	609, 618, 879, 1191, 1424, 1629, 2394
MaeII	ACGT	3 cut(s)	2369, 2617, 2772
MaeIII	GTNAC	8 cut(s)	229, 457, 727, 940, 1246, 1582, 1865, 2825
MalI	GATC	9 cut(s)	390, 510, 1388, 1635, 1644, 1791, 2443, 2644, 2697
MbiI	CCGCTC	1 cut(s)	1432
MboI	GATC	9 cut(s)	388, 508, 1386, 1633, 1642, 1789, 2441, 2642, 2695
MfeI	CAATTG	3 cut(s)	1176, 1269, 1913
MflI	RGATCY	3 cut(s)	508, 1642, 2642
MhlI	GDGCHC	2 cut(s)	894, 1513
MlyI	GAGTC	7 cut(s)	640, 746, 937, 1243, 1550, 1751, 2795
MmeI	TCCRAC	1 cut(s)	1018
Mph1103I	ATGCAT	2 cut(s)	670, 1321
MseI	TTAA	8 cut(s)	764, 1140, 1392, 1415, 1446, 1758, 2211, 2259
MslI	CAYNNNNRTG	2 cut(s)	1863, 2823
MspA1I	CMGCKG	2 cut(s)	26, 860
MspI	CCGG	3 cut(s)	1098, 1939, 2743
MunI	CAATTG	3 cut(s)	1176, 1269, 1913
Mva1269I	GAATGC	2 cut(s)	297, 1319
MvaI	CCWGG	9 cut(s)	81, 171, 288, 915, 1005, 1334, 1366, 1853, 2299
MvnI	CGCG	1 cut(s)	2010
NciI	CCSGG	1 cut(s)	1940
NcoI	CCATGG	1 cut(s)	1020
NdeI	CATATG	1 cut(s)	567
NdeII	GATC	9 cut(s)	388, 508, 1386, 1633, 1642, 1789, 2441, 2642, 2695
NlaIV	GGNNCC	4 cut(s)	48, 85, 892, 1155
NmeAIII	GCCGAG	1 cut(s)	246
NmuCI	GTSAC	4 cut(s)	940, 1246, 1865, 2825
NsiI	ATGCAT	2 cut(s)	670, 1321
NspI	RCATGY	2 cut(s)	872, 1973
PaeR7I	CTCGAG	1 cut(s)	925
PagI	TCATGA	1 cut(s)	2692
PciSI	GCTCTTC	1 cut(s)	1695
PctI	GAATGC	2 cut(s)	297, 1319
PfeI	GAWTC	2 cut(s)	1337, 1608
PflFI	GACNNNGTC	1 cut(s)	111
PfoI	TCCNGGA	2 cut(s)	1332, 1851
PinAI	ACCGGT	1 cut(s)	1097
PleI	GAGTC	7 cut(s)	640, 745, 936, 1243, 1549, 1750, 2795
PpsI	GAGTC	7 cut(s)	640, 745, 936, 1243, 1549, 1750, 2795
PshBI	ATTAAT	1 cut(s)	1392
Psp6I	CCWGG	9 cut(s)	79, 169, 286, 913, 1003, 1332, 1364, 1851, 2297
PspFI	CCCAGC	5 cut(s)	336, 476, 893, 1232, 2397
PspGI	CCWGG	9 cut(s)	79, 169, 286, 913, 1003, 1332, 1364, 1851, 2297
PspN4I	GGNNCC	4 cut(s)	48, 85, 892, 1155
PspOMI	GGGCCC	1 cut(s)	890
PspPI	GGNCC	6 cut(s)	890, 891, 1362, 1459, 1855, 2508
PstI	CTGCAG	3 cut(s)	517, 700, 1054
PstNI	CAGNNNCTG	2 cut(s)	512, 1232
PsuI	RGATCY	3 cut(s)	508, 1642, 2642
PsyI	GACNNNGTC	1 cut(s)	111
PvuII	CAGCTG	2 cut(s)	26, 860
RseI	CAYNNNNRTG	2 cut(s)	1863, 2823
SapI	GCTCTTC	1 cut(s)	1695
SaqAI	TTAA	8 cut(s)	764, 1140, 1392, 1415, 1446, 1758, 2211, 2259
Sau3AI	GATC	9 cut(s)	388, 508, 1386, 1633, 1642, 1789, 2441, 2642, 2695
Sau96I	GGNCC	6 cut(s)	890, 891, 1362, 1459, 1855, 2508
ScaI	AGTACT	2 cut(s)	607, 1454
SchI	GAGTC	7 cut(s)	640, 746, 937, 1243, 1550, 1751, 2795
SduI	GDGCHC	2 cut(s)	894, 1513
SexAI	ACCWGGT	1 cut(s)	79
SfaNI	GCATC	6 cut(s)	273, 655, 677, 1802, 2146, 2761
SfcI	CTRYAG	3 cut(s)	513, 696, 1050
Sfr274I	CTCGAG	1 cut(s)	925
SinI	GGWCC	2 cut(s)	1362, 1855
SlaI	CTCGAG	1 cut(s)	925
SmiMI	CAYNNNNRTG	2 cut(s)	1863, 2823
SmlI	CTYRAG	4 cut(s)	384, 925, 1158, 1670
SmoI	CTYRAG	4 cut(s)	384, 925, 1158, 1670
SsiI	CCGC	7 cut(s)	1432, 1837, 1921, 2329, 2581, 2749, 2830
SspI	AATATT	1 cut(s)	128
SspMI	CTAG	7 cut(s)	609, 618, 879, 1191, 1424, 1629, 2394
StyI	CCWWGG	3 cut(s)	422, 1020, 1545
TaaI	ACNGT	9 cut(s)	369, 676, 922, 1982, 2018, 2043, 2127, 2386, 2674
TaiI	ACGT	3 cut(s)	2372, 2620, 2775
TaqI	TCGA	2 cut(s)	37, 926
TatI	WGTACW	6 cut(s)	605, 687, 1009, 1348, 1452, 1725
TauI	GCSGC	2 cut(s)	2332, 2752
TfiI	GAWTC	2 cut(s)	1337, 1608
Tru1I	TTAA	8 cut(s)	764, 1140, 1392, 1415, 1446, 1758, 2211, 2259
Tru9I	TTAA	8 cut(s)	764, 1140, 1392, 1415, 1446, 1758, 2211, 2259
TscAI	CASTG	5 cut(s)	657, 945, 1059, 1778, 2009
TseFI	GTSAC	4 cut(s)	940, 1246, 1865, 2825
TseI	GCWGC	8 cut(s)	26, 142, 452, 472, 1694, 1973, 2057, 2216
Tsp45I	GTSAC	4 cut(s)	940, 1246, 1865, 2825
TspGWI	ACGGA	1 cut(s)	2539
TspRI	CASTG	5 cut(s)	657, 945, 1059, 1778, 2009
Tth111I	GACNNNGTC	1 cut(s)	111
VpaK11BI	GGWCC	2 cut(s)	1362, 1855
VspI	ATTAAT	1 cut(s)	1392
XagI	CCTNNNNNAGG	1 cut(s)	1874
XapI	RAATTY	4 cut(s)	1520, 1733, 2207, 2472
XceI	RCATGY	2 cut(s)	872, 1973
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2770
XhoI	CTCGAG	1 cut(s)	925
XspI	CTAG	7 cut(s)	609, 618, 879, 1191, 1424, 1629, 2394
ZrmI	AGTACT	2 cut(s)	607, 1454
Zsp2I	ATGCAT	2 cut(s)	670, 1321

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.