Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009577

Physical Location & Seq

Genomic Coordinates Forward (+)

6536620 .. 6547739

Gene Structure 11120 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1078500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3060 bp

ATGGCGAATAACCCTCAGTCCTCAGCTGGGCAGCCATTTCGTCCTCCCCCAGTTGCTTCCATGGGTCCTCAAAGTTTTGGTTCATCCCCACTTCAATACCGACCAGTGGTTCCAACACAGCAAGGACAGCAATTTATTCCACCAGCTTCCCAACAATTTCAGCCTGTGGGACAAGGTATTCCTTCTCCAAATGTTGGGATGCCTGGTAGTCAAAGTCAGCAATTACAATATTCTCAACCAATGCAACCGTATCCTCTCAGGCCAAGTCAGCCTGGTCATGCTCATTCGTCACAGGCGTTGCCTATGCAATACATGCAGACAAGGCCTACTGTCCCATCTCAGTCTCAGCAACCTGTCCCACCTTTTAATAATCAGATGCCTGGAATGCCTTATTCTTCCTCATATATGTTCGCTCCGCCTACGTATGTCCAGCAACAAAATAATGTCAGTTCACAATTTCAGCCGATGTCTCAAGTGCAAGCACACGTTGTATCTGCCACAGGACAGCCTTGGGTGTCCTCTGGTAATCAGGGTGCAGCAGTTCCTTCACCAGTACAGCAGCCTGGCCAACAATCCTCAAGCACTCTTTCCACAGATTCTGCTGTAAATGTTCCTAGCCAAATACAGCAGTCTTCTTCGGATTGGCAAGAGCATATGGCGGCTGATGGAAGAAGATATTATTTCAACAGAAGAACAAGAGAATCTAATTGGGAGAAGCCTTTAGAGTTGATGACACCGATGGAGAGGGCCGATGCATCAACTGTTTGGAAAGAGTATACGTCTGCAGATGGGAAAAAGTATTATTACAACAAGGTTACAAGGGAGTCTAAGTGGACAATTCCAGAGGAGTTAAAGTTGGCTCGCGAACAGGCTCAAAGAGAACTAATCCAAGGAACACATTCAGAAATGAATTTGACTTCTCATACCCCTCCTGCTGTTGCTTCAGCTGAAACACCTATGGGAACTAGTTCAGTTGGCCCTAGCACTTCTTCAGCCCTACCCGGGATGGTCCCGAGCCCTGTTGCAGTTACACCTGTGTCTTCTTTTTCTAATCCTTCTCCGACTGCACCTACTGGATCATCAGTTGCTTCTGGTGCAGAGTCTTCCATAACAGGTGCTGTTGGCGTTCAACCTCCTGCAGTAACTGTGACTCCAGTGCCTGCTTCTGTTTCAGGGAGTACCGGAGTTCCTCCTACTTCGGTCAACACCACTACAAATTCAGTGAGTACTTATGAAACCGTAGCATCTCAAGATATTGCAAGTTCAGCTGCTGATGGAACTTTGACTCAAGATATCGAGGAAGCCAAAAGAGGTATGGCAGTTGCTGGAAAAGTCAATGTGACTCCTTCAGAGGAGAAAACATTTGATGACGAACCACTGGTGTATGCCAATAAGCAGGAGGCAAAAACTGCTTTCAAATCACTTCTGGAATCTGCAAATGTCCAATCTGATTGGACTTGGGAGCAGACAATGAGGGAGATCATTACTGACAAAAGATACGGCGCTTTAAAAACTCTAGGTGAGAGGAAGCAAGCATTTAATGAGTACTTGGGTCAAAGGAAAAAACTGGAGAATGAAGAGAGGCGCATGAGGCAGAAAAAAGCTCGGGAAGAATTCACAAAAATGTTGGAAGAGTCCAGGGAACTTACATCAGCAACCAGATGGAGCAAAGCTGTGAGTATGTTTGAAAATGATGAACGATTCAAGGCTGTAGAACGAGCTAGAGACCGGGAAGATCTTTATGAGAGCTACATTGTAGAACTCGAAAGGAAGGAAAAACAAAAGGCAGCTGAAGAGCATAAGCAGAATATTGTAGAGTACAGGAAATTTCTGGAATCTTGTGACTTTATCAAGGTGAACAGCCAGTGGCGGAAAGTTCAAGATCGCTTGGAGGATGATGAGAGATGCTTGCGCCTTGAAAAGCTTGCTAGGTTGCTCATTTTCCAGGACTATATTCGTGACCTTGAGAAGGTGGAAGAGGAGCAAAAAAAGTTACAGAAGGAACAATTGAGGCGGGAAGAGAGGAAAAACCGTGATGAGTTCCGCAAGCTGTTGGAAGAACATGTTGCTGATGGCACTCTTACAGCTAAAACTCTCTGGCGCGATTACTGTACGAAGGTTAAAGACTTGCCTCCATATGAAGCTGTTGCATTGAACACCTCTGGTTCAACACCAAAAGAATTGTTTGATGATGTTTTTGAAGAATTAGAGAAACAGTATCATGACGATAAAGCTCGGATAAAGGATGCAGTGAAGTCAGGGAAGGTTACCATGACTTCCACATTGGCATTTGAAGAGTTTAAGGTTCCTATTCTGGAAGATATTGGTTCTCCACCAATATCAGACATTAACTTCAAGCTTGTGTATGAGGAATTATTAGAGAGAGCCAAGGAGAAGGAGGAGAAAGAGGCAAAGAAGCGTCAACGACTTGTTGATGACTTCAATAACCTATTGCACACAATCAAGGAAATAAGTGCTGCTTCTAATTGGGAAGATAGCAAACACCTTTTTGAAGAAACCCAAGAATACAGATCAATTGGTGACGGTAGGGAAGTTTTTGAGGAATATATTACAAACTTACAGGAGAGGGCTAAAGAGAAGGAACGAAAGCGTGAGGAGGAAAAGGCCAAAAAAGAGAAAGAAAGAGAAGAGAAAGAGAAGCGGAAGGATAAAGAGAGAAAGGAGAAGGAAAGAGAACGTGAAAAAGAAAAGGGAAAGGACCGATCTAAGAAGGATGAAACAGATAGTGAAAATGTAGACATAACTGAGAGTCATGGGCATAAAGAAGATAAGAAGAGGGAGAAAGACAAAGACAGAAAGCACCGGAAGCGGCATCAAAGTTCCATTGATGATGTAGGTTCCGATAAGGAGGAGAAAGAGGAGTCTAAGAAACACAGACACAGTAGTGACCGCAAAAGATCAAGGAAACATACACCTGAATCTGACAGTGAAAGTCGGCATAGAAGGCATAAGAGAGAGCATCGGGATGGTTCCCGTCGAAGTGGTGAACATGTCGACCTTGAAGACGGGGAGCTTGGTGAAGATGGGGAAATTAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005685 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006928 GO:0006996 GO:0007010 GO:0008150 GO:0008152 GO:0008360 GO:0008380 GO:0009987 GO:0010467 GO:0010564 GO:0016043 GO:0016070 GO:0016071 GO:0016363 GO:0016477 GO:0016592 GO:0016604 GO:0016607 GO:0022603 GO:0022604 GO:0030532 GO:0031974 GO:0031981 GO:0032465 GO:0032991 GO:0034399 GO:0034641 GO:0040011 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051302 GO:0051674 GO:0051726 GO:0065007 GO:0065008 GO:0070013 GO:0070064 GO:0071004 GO:0071010 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097525 GO:0120114 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

FF WW

Protein Analysis

1020

Amino Acids

116.17

Weight (kDa)

6.29

Isoelectric Point (pI)

59.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	213 - 240	4.6e-08	WW domain
WW	PF00397	256 - 281	1.2e-07	WW domain
FF	PF01846	467 - 516	1.2e-15	FF domain
FF	PF01846	534 - 584	4.6e-13	FF domain
FF	PF01846	676 - 731	2e-07	FF domain
FF_PRPF40A	PF25432	739 - 798	1.2e-21	PRPF40A-like, FF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44910 AT1G44910
fragaria_vesca	FvH4_2g32680 FvH4_2g32680 FvH4_2g32680 FvH4_2g32680
malus_domestica	MD08G1078500.v1.1 MD15G1065300.v1.1
prunus_persica	Prupe.1G419400_v2.0.a1
pyrus_communis	pycom08g06490 pycom15g06180
rosa_chinensis	RchiOBHm_Chr6g0309991
rosa_laevigata	RLG00000010518
rosa_multiflora	Rmu_sc0000294.1_g000011
rosa_roxburghii	Rroxscaffold_7G00158970
rosa_rugosa	Rorug06G0376700 Rorug06G0376800
rosa_samantha	Rh6AG490400 Rh6BG499900 Rh6CG504800 Rh6DG490800
rosa_wichuraiana	Rw6G042690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	194
AccI	GTMKAC	3 cut(s)	776, 2757, 3015
AccII	CGCG	2 cut(s)	864, 2103
AciI	CCGC	8 cut(s)	416, 659, 1870, 2014, 2044, 2662, 2830, 2911
AclWI	GGATC	1 cut(s)	1084
AcoI	YGGCCR	1 cut(s)	565
AcsI	RAATTY	4 cut(s)	910, 1216, 1613, 1826
AcuI	CTGAAG	4 cut(s)	927, 975, 1332, 1812
AfaI	GTAC	6 cut(s)	555, 1180, 1228, 1547, 1820, 2113
AfiI	CCNNNNNNNGG	5 cut(s)	27, 106, 194, 1002, 1969
AflIII	ACRYGT	3 cut(s)	484, 2062, 3010
AhlI	ACTAGT	1 cut(s)	965
AjnI	CCWGG	6 cut(s)	202, 271, 379, 562, 1637, 1944
AleI	CACNNNNGTG	1 cut(s)	2904
Alw26I	GTCTC	3 cut(s)	348, 474, 1719
AlwI	GGATC	1 cut(s)	1084
AlwNI	CAGNNNCTG	6 cut(s)	599, 1118, 1145, 1160, 1271, 1325
Ama87I	CYCGRG	3 cut(s)	1001, 1012, 1605
AoxI	GGCC	6 cut(s)	260, 323, 565, 747, 976, 2625
ApeKI	GCWGC	6 cut(s)	31, 536, 559, 1268, 1787, 2477
ApoI	RAATTY	4 cut(s)	910, 1216, 1613, 1826
Asp700I	GAANNNNTTC	2 cut(s)	898, 967
AspLEI	GCGC	4 cut(s)	1505, 1587, 1914, 2103
AspS9I	GGNCC	5 cut(s)	65, 747, 977, 1009, 2719
AsuC2I	CCSGG	3 cut(s)	1002, 1003, 1730
AsuHPI	GGTGA	6 cut(s)	540, 1532, 1867, 2552, 3017, 3050
AvaI	CYCGRG	3 cut(s)	1001, 1012, 1605
AvaII	GGWCC	3 cut(s)	65, 1009, 2719
BalI	TGGCCA	1 cut(s)	567
BanII	GRGCYC	1 cut(s)	1019
BbsI	GAAGAC	4 cut(s)	624, 1032, 1095, 3030
BbvCI	CCTCAGC	1 cut(s)	22
BbvI	GCAGC	6 cut(s)	43, 548, 571, 1255, 1799, 2464
BceAI	ACGGC	1 cut(s)	1516
BcgI	CGANNNNNNTGC	2 cut(s)	20, 54
BciT130I	CCWGG	6 cut(s)	204, 273, 381, 564, 1639, 1946
BciVI	GTATCC	1 cut(s)	261
BcnI	CCSGG	3 cut(s)	1002, 1003, 1730
BcoDI	GTCTC	3 cut(s)	348, 474, 1719
BcuI	ACTAGT	1 cut(s)	965
BfaI	CTAG	6 cut(s)	615, 966, 981, 1517, 1722, 1929
BfmI	CTRYAG	3 cut(s)	783, 1137, 1710
BfoI	RGCGCY	1 cut(s)	1506
BfuI	GTATCC	1 cut(s)	261
BglII	AGATCT	1 cut(s)	1735
BisI	GCNGC	8 cut(s)	32, 537, 560, 660, 1269, 1788, 2478, 2831
BlsI	GCNGC	8 cut(s)	33, 538, 561, 661, 1270, 1789, 2479, 2832
BmcAI	AGTACT	2 cut(s)	1228, 1547
Bme1390I	CCNGG	9 cut(s)	204, 273, 381, 564, 1002, 1003, 1639, 1730, 1946
Bme18I	GGWCC	3 cut(s)	65, 1009, 2719
BmeT110I	CYCGRG	3 cut(s)	1001, 1012, 1605
BmgT120I	GGNCC	5 cut(s)	65, 747, 977, 1009, 2719
BmiI	GGNNCC	6 cut(s)	66, 111, 1011, 2307, 2860, 2992
BmrFI	CCNGG	9 cut(s)	204, 273, 381, 564, 1002, 1003, 1639, 1730, 1946
BmrI	ACTGGG	1 cut(s)	44
BmsI	GCATC	9 cut(s)	189, 366, 742, 764, 1253, 1895, 2236, 2842, 2989
BmuI	ACTGGG	1 cut(s)	44
BpiI	GAAGAC	4 cut(s)	624, 1032, 1095, 3030
BpmI	CTGGAG	2 cut(s)	1137, 1589
Bpu10I	CCTNAGC	1 cut(s)	22
BpuEI	CTTGAG	5 cut(s)	456, 562, 1233, 1272, 1985
BpuMI	CCSGG	3 cut(s)	1002, 1003, 1730
BsaAI	YACGTR	1 cut(s)	423
BsaI	GGTCTC	1 cut(s)	1719
BsaJI	CCNNGG	6 cut(s)	60, 509, 889, 1001, 1638, 2388
BsaWI	WCCGGW	2 cut(s)	1181, 2823
BsaXI	ACNNNNNCTCC	6 cut(s)	815, 839, 845, 869, 1135, 1165
Bsc4I	CCNNNNNNNGG	5 cut(s)	27, 106, 194, 1002, 1969
Bse1I	ACTGG	8 cut(s)	50, 104, 551, 1078, 1154, 1383, 1572, 1864
BseBI	CCWGG	6 cut(s)	204, 273, 381, 564, 1639, 1946
BseDI	CCNNGG	6 cut(s)	60, 509, 889, 1001, 1638, 2388
BseGI	GGATG	7 cut(s)	83, 204, 1011, 1900, 2251, 2740, 2992
BseLI	CCNNNNNNNGG	5 cut(s)	27, 106, 194, 1002, 1969
BseMII	CTCAG	6 cut(s)	29, 36, 271, 353, 359, 2757
BseNI	ACTGG	8 cut(s)	50, 104, 551, 1078, 1154, 1383, 1572, 1864
BseRI	GAGGAG	7 cut(s)	860, 1367, 1994, 2414, 2630, 2885, 2894
BseXI	GCAGC	6 cut(s)	43, 548, 571, 1255, 1799, 2464
BseYI	CCCAGC	1 cut(s)	26
BsgI	GTGCAG	3 cut(s)	555, 1050, 1116
Bsh1236I	CGCG	2 cut(s)	864, 2103
BshFI	GGCC	6 cut(s)	262, 325, 567, 749, 978, 2627
BsiHKCI	CYCGRG	3 cut(s)	1001, 1012, 1605
BsiSI	CCGG	4 cut(s)	1002, 1182, 1729, 2824
BslFI	GGGAC	4 cut(s)	183, 317, 341, 995
BslI	CCNNNNNNNGG	5 cut(s)	27, 106, 194, 1002, 1969
BsmAI	GTCTC	3 cut(s)	348, 474, 1719
BsmFI	GGGAC	4 cut(s)	183, 317, 341, 995
BsmI	GAATGC	1 cut(s)	390
BsnI	GGCC	6 cut(s)	262, 325, 567, 749, 978, 2627
Bso31I	GGTCTC	1 cut(s)	1719
BsoBI	CYCGRG	3 cut(s)	1001, 1012, 1605
Bsp1286I	GDGCHC	1 cut(s)	1019
Bsp143I	GATC	7 cut(s)	1076, 1479, 1735, 1882, 2531, 2723, 2918
Bsp19I	CCATGG	1 cut(s)	60
Bsp68I	TCGCGA	1 cut(s)	864
BspACI	CCGC	8 cut(s)	416, 659, 1870, 2014, 2044, 2662, 2830, 2911
BspANI	GGCC	6 cut(s)	262, 325, 567, 749, 978, 2627
BspCNI	CTCAG	6 cut(s)	28, 35, 270, 352, 358, 2758
BspFNI	CGCG	2 cut(s)	864, 2103
BspHI	TCATGA	1 cut(s)	2221
BspLI	GGNNCC	6 cut(s)	66, 111, 1011, 2307, 2860, 2992
BspMAI	CTGCAG	2 cut(s)	787, 1141
BspPI	GGATC	1 cut(s)	1084
BspQI	GCTCTTC	1 cut(s)	1788
BspTNI	GGTCTC	1 cut(s)	1719
BsrI	ACTGG	8 cut(s)	50, 104, 551, 1078, 1154, 1383, 1572, 1864
BssECI	CCNNGG	6 cut(s)	60, 509, 889, 1001, 1638, 2388
BssMI	GATC	7 cut(s)	1076, 1479, 1735, 1882, 2531, 2723, 2918
BssNAI	GTATAC	1 cut(s)	777
BssT1I	CCWWGG	4 cut(s)	60, 509, 889, 2388
Bst1107I	GTATAC	1 cut(s)	777
Bst2UI	CCWGG	6 cut(s)	204, 273, 381, 564, 1639, 1946
Bst6I	CTCTTC	8 cut(s)	1572, 1626, 1788, 1971, 2013, 2289, 2643, 2789
BstAPI	GCANNNNNTGC	2 cut(s)	313, 1409
BstBAI	YACGTR	1 cut(s)	423
BstC8I	GCNNGC	7 cut(s)	480, 862, 1161, 1533, 1910, 1926, 2048
BstDEI	CTNAG	9 cut(s)	15, 22, 257, 339, 345, 828, 2727, 2766, 2886
BstDSI	CCRYGG	1 cut(s)	60
BstEII	GGTNACC	1 cut(s)	2266
BstENI	CCTNNNNNAGG	1 cut(s)	1967
BstF5I	GGATG	7 cut(s)	83, 204, 1011, 1900, 2251, 2740, 2992
BstFNI	CGCG	2 cut(s)	864, 2103
BstH2I	RGCGCY	1 cut(s)	1506
BstHHI	GCGC	4 cut(s)	1505, 1587, 1914, 2103
BstKTI	GATC	7 cut(s)	1079, 1482, 1738, 1885, 2534, 2726, 2921
BstMAI	GTCTC	3 cut(s)	348, 474, 1719
BstMBI	GATC	7 cut(s)	1076, 1479, 1735, 1882, 2531, 2723, 2918
BstNI	CCWGG	6 cut(s)	204, 273, 381, 564, 1639, 1946
BstNSI	RCATGY	3 cut(s)	316, 2066, 3014
BstPI	GGTNACC	1 cut(s)	2266
BstSCI	CCNGG	9 cut(s)	202, 271, 379, 562, 1000, 1001, 1637, 1728, 1944
BstSFI	CTRYAG	3 cut(s)	783, 1137, 1710
BstSNI	TACGTA	1 cut(s)	423
BstUI	CGCG	2 cut(s)	864, 2103
BstV1I	GCAGC	6 cut(s)	43, 548, 571, 1255, 1799, 2464
BstV2I	GAAGAC	4 cut(s)	624, 1032, 1095, 3030
BstX2I	RGATCY	1 cut(s)	1735
BstYI	RGATCY	1 cut(s)	1735
BstZ17I	GTATAC	1 cut(s)	777
BsuI	GTATCC	1 cut(s)	261
BsuRI	GGCC	6 cut(s)	262, 325, 567, 749, 978, 2627
BtgI	CCRYGG	1 cut(s)	60
BtsCI	GGATG	7 cut(s)	83, 204, 1011, 1900, 2251, 2740, 2992
BtsI	GCAGTG	1 cut(s)	2256
BtsIMutI	CAGTG	7 cut(s)	111, 1161, 1227, 1376, 1871, 2256, 2953
BtuMI	TCGCGA	1 cut(s)	864
Cac8I	GCNNGC	7 cut(s)	480, 862, 1161, 1533, 1910, 1926, 2048
CaiI	CAGNNNCTG	6 cut(s)	599, 1118, 1145, 1160, 1271, 1325
CciI	TCATGA	1 cut(s)	2221
CfoI	GCGC	4 cut(s)	1505, 1587, 1914, 2103
Cfr13I	GGNCC	5 cut(s)	65, 747, 977, 1009, 2719
Cfr9I	CCCGGG	1 cut(s)	1001
CseI	GACGC	1 cut(s)	2408
Csp6I	GTAC	6 cut(s)	554, 1179, 1227, 1546, 1819, 2112
CviAII	CATG	9 cut(s)	61, 278, 313, 1588, 2063, 2222, 2272, 2774, 3011
CviQI	GTAC	6 cut(s)	554, 1179, 1227, 1546, 1819, 2112
DdeI	CTNAG	9 cut(s)	15, 22, 257, 339, 345, 828, 2727, 2766, 2886
DpnI	GATC	7 cut(s)	1078, 1481, 1737, 1884, 2533, 2725, 2920
DpnII	GATC	7 cut(s)	1076, 1479, 1735, 1882, 2531, 2723, 2918
DraI	TTTAAA	1 cut(s)	1509
EaeI	YGGCCR	1 cut(s)	565
Eam1104I	CTCTTC	8 cut(s)	1572, 1626, 1788, 1971, 2013, 2289, 2643, 2789
EarI	CTCTTC	8 cut(s)	1572, 1626, 1788, 1971, 2013, 2289, 2643, 2789
EciI	GGCGGA	2 cut(s)	405, 1885
Eco105I	TACGTA	1 cut(s)	423
Eco130I	CCWWGG	4 cut(s)	60, 509, 889, 2388
Eco147I	AGGCCT	1 cut(s)	325
Eco24I	GRGCYC	1 cut(s)	1019
Eco31I	GGTCTC	1 cut(s)	1719
Eco32I	GATATC	1 cut(s)	1294
Eco47I	GGWCC	3 cut(s)	65, 1009, 2719
Eco57I	CTGAAG	4 cut(s)	927, 975, 1332, 1812
Eco88I	CYCGRG	3 cut(s)	1001, 1012, 1605
Eco91I	GGTNACC	1 cut(s)	2266
EcoNI	CCTNNNNNAGG	1 cut(s)	1967
EcoO109I	RGGNCCY	1 cut(s)	65
EcoO65I	GGTNACC	1 cut(s)	2266
EcoRI	GAATTC	1 cut(s)	1613
EcoRII	CCWGG	6 cut(s)	202, 271, 379, 562, 1637, 1944
EcoRV	GATATC	1 cut(s)	1294
EcoT14I	CCWWGG	4 cut(s)	60, 509, 889, 2388
EcoT22I	ATGCAT	1 cut(s)	757
EcoT38I	GRGCYC	1 cut(s)	1019
ErhI	CCWWGG	4 cut(s)	60, 509, 889, 2388
FaeI	CATG	9 cut(s)	64, 281, 316, 1591, 2066, 2225, 2275, 2777, 3014
FalI	AAGNNNNNCTT	2 cut(s)	2464, 2496
FaqI	GGGAC	4 cut(s)	183, 317, 341, 995
FatI	CATG	9 cut(s)	60, 277, 312, 1587, 2062, 2221, 2271, 2773, 3010
FauI	CCCGC	1 cut(s)	2007
FauNDI	CATATG	2 cut(s)	654, 2137
FblI	GTMKAC	3 cut(s)	776, 2757, 3015
Fnu4HI	GCNGC	8 cut(s)	32, 537, 560, 660, 1269, 1788, 2478, 2831
FokI	GGATG	7 cut(s)	70, 211, 1018, 1907, 2258, 2747, 2999
FriOI	GRGCYC	1 cut(s)	1019
Fsp4HI	GCNGC	8 cut(s)	32, 537, 560, 660, 1269, 1788, 2478, 2831
FspBI	CTAG	6 cut(s)	615, 966, 981, 1517, 1722, 1929
GlaI	GCGC	4 cut(s)	1504, 1586, 1913, 2102
GluI	GCNGC	8 cut(s)	32, 537, 560, 660, 1269, 1788, 2478, 2831
GsaI	CCCAGC	1 cut(s)	30
GsuI	CTGGAG	2 cut(s)	1137, 1589
HaeII	RGCGCY	1 cut(s)	1506
HaeIII	GGCC	6 cut(s)	262, 325, 567, 749, 978, 2627
HapII	CCGG	4 cut(s)	1002, 1182, 1729, 2824
HgaI	GACGC	1 cut(s)	2408
HhaI	GCGC	4 cut(s)	1505, 1587, 1914, 2103
Hin1II	CATG	9 cut(s)	64, 281, 316, 1591, 2066, 2225, 2275, 2777, 3014
Hin6I	GCGC	4 cut(s)	1503, 1585, 1912, 2101
HinP1I	GCGC	4 cut(s)	1503, 1585, 1912, 2101
HincII	GTYRAC	3 cut(s)	1204, 2423, 3016
HindII	GTYRAC	3 cut(s)	1204, 2423, 3016
HindIII	AAGCTT	2 cut(s)	1922, 2357
HpaII	CCGG	4 cut(s)	1002, 1182, 1729, 2824
HphI	GGTGA	6 cut(s)	540, 1532, 1867, 2552, 3017, 3050
Hpy166II	GTNNAC	9 cut(s)	452, 777, 834, 1204, 1858, 2423, 2758, 3008, 3016
Hpy8I	GTNNAC	9 cut(s)	452, 777, 834, 1204, 1858, 2423, 2758, 3008, 3016
Hpy99I	CGWCG	1 cut(s)	3000
HpyCH4IV	ACGT	4 cut(s)	422, 486, 779, 2698
HpyF3I	CTNAG	9 cut(s)	15, 22, 257, 339, 345, 828, 2727, 2766, 2886
HpySE526I	ACGT	4 cut(s)	422, 486, 779, 2698
Hsp92II	CATG	9 cut(s)	64, 281, 316, 1591, 2066, 2225, 2275, 2777, 3014
HspAI	GCGC	4 cut(s)	1503, 1585, 1912, 2101
Kzo9I	GATC	7 cut(s)	1076, 1479, 1735, 1882, 2531, 2723, 2918
LguI	GCTCTTC	1 cut(s)	1788
LmnI	GCTCC	5 cut(s)	418, 1462, 1665, 1981, 3031
Lsp1109I	GCAGC	6 cut(s)	43, 548, 571, 1255, 1799, 2464
LweI	GCATC	9 cut(s)	189, 366, 742, 764, 1253, 1895, 2236, 2842, 2989
MaeI	CTAG	6 cut(s)	615, 966, 981, 1517, 1722, 1929
MaeII	ACGT	4 cut(s)	422, 486, 779, 2698
MalI	GATC	7 cut(s)	1078, 1481, 1737, 1884, 2533, 2725, 2920
MboI	GATC	7 cut(s)	1076, 1479, 1735, 1882, 2531, 2723, 2918
MfeI	CAATTG	2 cut(s)	2006, 2535
MflI	RGATCY	1 cut(s)	1735
MhlI	GDGCHC	1 cut(s)	1019
MlsI	TGGCCA	1 cut(s)	567
MluNI	TGGCCA	1 cut(s)	567
MlyI	GAGTC	8 cut(s)	833, 1109, 1144, 1279, 1336, 1643, 2779, 2891
MmeI	TCCRAC	4 cut(s)	137, 1085, 1608, 2034
Mox20I	TGGCCA	1 cut(s)	567
Mph1103I	ATGCAT	1 cut(s)	757
MroXI	GAANNNNTTC	2 cut(s)	898, 967
MscI	TGGCCA	1 cut(s)	567
MseI	TTAA	8 cut(s)	366, 851, 1508, 1539, 2121, 2301, 2349, 3054
MslI	CAYNNNNRTG	3 cut(s)	1622, 2904, 2985
Msp20I	TGGCCA	1 cut(s)	567
MspA1I	CMGCKG	4 cut(s)	26, 947, 1268, 1790
MspI	CCGG	4 cut(s)	1002, 1182, 1729, 2824
MspR9I	CCNGG	9 cut(s)	204, 273, 381, 564, 1002, 1003, 1639, 1730, 1946
MunI	CAATTG	2 cut(s)	2006, 2535
Mva1269I	GAATGC	1 cut(s)	390
MvaI	CCWGG	6 cut(s)	204, 273, 381, 564, 1639, 1946
MvnI	CGCG	2 cut(s)	864, 2103
NciI	CCSGG	3 cut(s)	1002, 1003, 1730
NcoI	CCATGG	1 cut(s)	60
NdeI	CATATG	2 cut(s)	654, 2137
NdeII	GATC	7 cut(s)	1076, 1479, 1735, 1882, 2531, 2723, 2918
NlaIII	CATG	9 cut(s)	64, 281, 316, 1591, 2066, 2225, 2275, 2777, 3014
NlaIV	GGNNCC	6 cut(s)	66, 111, 1011, 2307, 2860, 2992
NmuCI	GTSAC	7 cut(s)	288, 1147, 1339, 1841, 1958, 2540, 2906
NruI	TCGCGA	1 cut(s)	864
NsiI	ATGCAT	1 cut(s)	757
NspI	RCATGY	3 cut(s)	316, 2066, 3014
OliI	CACNNNNGTG	1 cut(s)	2904
PagI	TCATGA	1 cut(s)	2221
PceI	AGGCCT	1 cut(s)	325
PciI	ACATGT	2 cut(s)	2062, 3010
PciSI	GCTCTTC	1 cut(s)	1788
PcsI	WCGNNNNNNNCGW	1 cut(s)	293
PctI	GAATGC	1 cut(s)	390
PdmI	GAANNNNTTC	2 cut(s)	898, 967
PfeI	GAWTC	6 cut(s)	596, 701, 1430, 1701, 1835, 2939
PflMI	CCANNNNNTGG	1 cut(s)	194
PfoI	TCCNGGA	1 cut(s)	1944
PkrI	GCNGC	8 cut(s)	33, 538, 561, 661, 1270, 1789, 2479, 2832
PleI	GAGTC	8 cut(s)	832, 1108, 1144, 1279, 1336, 1642, 2778, 2890
PpsI	GAGTC	8 cut(s)	832, 1108, 1144, 1279, 1336, 1642, 2778, 2890
Ppu21I	YACGTR	1 cut(s)	423
PpuMI	RGGWCCY	1 cut(s)	65
PscI	ACATGT	2 cut(s)	2062, 3010
Psp5II	RGGWCCY	1 cut(s)	65
Psp6I	CCWGG	6 cut(s)	202, 271, 379, 562, 1637, 1944
PspEI	GGTNACC	1 cut(s)	2266
PspFI	CCCAGC	1 cut(s)	26
PspGI	CCWGG	6 cut(s)	202, 271, 379, 562, 1637, 1944
PspN4I	GGNNCC	6 cut(s)	66, 111, 1011, 2307, 2860, 2992
PspPI	GGNCC	5 cut(s)	65, 747, 977, 1009, 2719
PspPPI	RGGWCCY	1 cut(s)	65
PstI	CTGCAG	2 cut(s)	787, 1141
PstNI	CAGNNNCTG	6 cut(s)	599, 1118, 1145, 1160, 1271, 1325
PsuI	RGATCY	1 cut(s)	1735
PvuII	CAGCTG	4 cut(s)	26, 947, 1268, 1790
RruI	TCGCGA	1 cut(s)	864
RsaI	GTAC	6 cut(s)	555, 1180, 1228, 1547, 1820, 2113
RsaNI	GTAC	6 cut(s)	554, 1179, 1227, 1546, 1819, 2112
RseI	CAYNNNNRTG	3 cut(s)	1622, 2904, 2985
SalI	GTCGAC	1 cut(s)	3014
SapI	GCTCTTC	1 cut(s)	1788
SaqAI	TTAA	8 cut(s)	366, 851, 1508, 1539, 2121, 2301, 2349, 3054
SatI	GCNGC	8 cut(s)	32, 537, 560, 660, 1269, 1788, 2478, 2831
Sau3AI	GATC	7 cut(s)	1076, 1479, 1735, 1882, 2531, 2723, 2918
Sau96I	GGNCC	5 cut(s)	65, 747, 977, 1009, 2719
ScaI	AGTACT	2 cut(s)	1228, 1547
SchI	GAGTC	8 cut(s)	833, 1109, 1144, 1279, 1336, 1643, 2779, 2891
ScrFI	CCNGG	9 cut(s)	204, 273, 381, 564, 1002, 1003, 1639, 1730, 1946
SduI	GDGCHC	1 cut(s)	1019
SfaNI	GCATC	9 cut(s)	189, 366, 742, 764, 1253, 1895, 2236, 2842, 2989
SfcI	CTRYAG	3 cut(s)	783, 1137, 1710
SinI	GGWCC	3 cut(s)	65, 1009, 2719
SmaI	CCCGGG	1 cut(s)	1003
SmiMI	CAYNNNNRTG	3 cut(s)	1622, 2904, 2985
SmlI	CTYRAG	5 cut(s)	471, 577, 1248, 1287, 1964
SmoI	CTYRAG	5 cut(s)	471, 577, 1248, 1287, 1964
SnaBI	TACGTA	1 cut(s)	423
SpeI	ACTAGT	1 cut(s)	965
SseBI	AGGCCT	1 cut(s)	325
SsiI	CCGC	8 cut(s)	416, 659, 1870, 2014, 2044, 2662, 2830, 2911
SspI	AATATT	2 cut(s)	230, 1810
SspMI	CTAG	6 cut(s)	615, 966, 981, 1517, 1722, 1929
StuI	AGGCCT	1 cut(s)	325
StyD4I	CCNGG	9 cut(s)	202, 271, 379, 562, 1000, 1001, 1637, 1728, 1944
StyI	CCWWGG	4 cut(s)	60, 509, 889, 2388
TaiI	ACGT	4 cut(s)	425, 489, 782, 2701
TaqI	TCGA	4 cut(s)	1296, 1764, 2998, 3015
TaqII	GACCGA	2 cut(s)	1189, 2736
TatI	WGTACW	4 cut(s)	553, 1226, 1545, 1818
TauI	GCSGC	2 cut(s)	662, 2833
TfiI	GAWTC	6 cut(s)	596, 701, 1430, 1701, 1835, 2939
Tru1I	TTAA	8 cut(s)	366, 851, 1508, 1539, 2121, 2301, 2349, 3054
Tru9I	TTAA	8 cut(s)	366, 851, 1508, 1539, 2121, 2301, 2349, 3054
TscAI	CASTG	7 cut(s)	111, 1161, 1227, 1383, 1871, 2256, 2953
TseFI	GTSAC	7 cut(s)	288, 1147, 1339, 1841, 1958, 2540, 2906
TseI	GCWGC	6 cut(s)	31, 536, 559, 1268, 1787, 2477
Tsp45I	GTSAC	7 cut(s)	288, 1147, 1339, 1841, 1958, 2540, 2906
TspDTI	ATGAA	7 cut(s)	72, 923, 1248, 1590, 1710, 2154, 2751
TspMI	CCCGGG	1 cut(s)	1001
TspRI	CASTG	7 cut(s)	111, 1161, 1227, 1383, 1871, 2256, 2953
Van91I	CCANNNNNTGG	1 cut(s)	194
VpaK11BI	GGWCC	3 cut(s)	65, 1009, 2719
XagI	CCTNNNNNAGG	1 cut(s)	1967
XapI	RAATTY	4 cut(s)	910, 1216, 1613, 1826
XceI	RCATGY	3 cut(s)	316, 2066, 3014
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1312
XmaI	CCCGGG	1 cut(s)	1001
XmiI	GTMKAC	3 cut(s)	776, 2757, 3015
XmnI	GAANNNNTTC	2 cut(s)	898, 967
XspI	CTAG	6 cut(s)	615, 966, 981, 1517, 1722, 1929
ZrmI	AGTACT	2 cut(s)	1228, 1547
Zsp2I	ATGCAT	1 cut(s)	757

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.