Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0009577

Physical Location & Seq

Genomic Coordinates Reverse (-)

66686550 .. 66697779

Gene Structure 11230 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG490400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2610 bp

ATGGCAAATAACCCTCAGTCCTCAGCTGCTCAGTATCGACCAATGGTACCGACACAGCAAGGACAACACTTTATTTCATCAGCTCCCCAACAATTTCAGCCTGTGGGACAAGGTCAGCCATTGCAATATTCTCAACAAATGCAGCCGTATCCTCTCAGGCCAAGTCAGCCTGGTCATGCTCAACCTTCATCACAGGCTTTACCTATGCCGTACTATCAGCCGAGGCCAGTTACATCTGTCCCATCACACTCTCAACAACCTGCCCCACCTTTTAATAATCAGATGCCTGGAATGCCTTATCCTTCATCATATATGTATGCACCGCCTTCTTTTGTCCAGCCACAGAATAATGTCAGTTCTTCATCACAGTTTCAGCCAATGTCTCAAGAGCAAGCACATGCTGTACCTGCTGCTGGACAACCTTGGATGTCCTCCAGTAATCATCAGGGTGCAGCAGTTGTTACACCACAACAGCAGCCTAGCCAGCAACCTACAAGCACTCCTTTCCCAGATCCTGCTGTAAATGTTCCTAATCTAACACAGCCGTCTGCTTCTGATTGGCAAGAACATATGGCAACTGATGGGAGAAGATATTACTTCAACAGAAGTACTAGACAATCCAGTTGGGAGAAACCTTTAGAATTGATGACTCCACTGGAGAGAGCCGATGCATCAACTGTTTGGAAAGAATATACTTCTGCAGATGGGAAAAAGTATTACTACAACAAGGTTACGAGGGAATCTAAATGGACAATTCCAGAGGAGTTGAAGCTGGCTCGTGAGCAAGCTCAAAGAGAACTTAATCAGGGAACACAATCAGAAATGAATTTGACTCCTCATGCTCCTCCTGCAACTGCTTCAGCTGAAATACATGCTGGAGCTAGTTCAGTTGGGCCCAGCACTTCTTCAGCCCAACCTGGGACAGTCTCGAGTCCAGTTGCAGTTACACCCATCTCTGCCTTCTCTAATCCTTCTCCTATTACGCCTTCGGGATCATTGGTTGCTCCTGGTGTACAGTCTTCCATGGCAACGGGTTCTGTTGGAGTTCAACCTGCCGTGGTGAGTGTGTCCCCACTGCCTGCTTCTAATTTGGGGAGTACTGGAGTTCCTTCTACTTTGGTCAACACCAATACAAAATCAGTAGGTAATGAAAATCAGGCATCTCAAGATATTGCAAGTTCAATTGATGGAGCTTTTACTCAAGATATTGAGGAAGCAAAGAAAGGAATGGCAGTTGCTGGAAAAGTCAACGTGACTCCTTCAGAGGAGAAAGCAATTGATGATGAGCCATTGGTGTATGCCAGTAAGCAGGAGGCAAAAAATGCATTCAAATCACTCCTGGAATCCGCAAATGTCCACTCTGATTGGACCTGGGAGCAGGCAATGAGAGAGATCATTAATGACAAAAGATATGGTGCTTTAAGAACACTGGGTGAGCGGAAGCAGGCTTTTAATGAGTACCTGGGCCAAAGGAAAAAGTTGGAGAATGAAGAGAGGCGTATCAGGCAAAAAAGGGCTCGGGAAGAATTTACAAAAATGCTAGAAGAGTCCAAGGAACTCACATCAACAATTAGATGGAGCAAAGCTGTAACTATGTTTGAAAATGATGAACGGTTTAAGGCTGTTGAACGAGCTAGAGATCGGGAAGATCTCTATGAGAGCTACATTGTAGAACTTGAGAGGAAGGAGAAAGAATACGCTGCTGAAGAGCATAGGAGGAATATATCAGAGTACAGGGAATTTCTGGAGTCTTGCGACTTTATTAAGGTGAACAGCCAATGGCGTAAAGTTCAAGATCGCTTAGAGGACGATGAGAGATGCTTGCGTCTTGAAAAGCTTGACCGCTTGCTCATTTTCCAGGACTATATTCGTGACCTTGAGAAGGAGGAAGAGGAGCAAAAGAAGATGCAAAAGGAACAATTGAGGCGGATTGAGAGGAAAAACCGGGATGAGTTTCGCAAGCTCTTGGAAGAACATGCTGCTGACGGTACTCTTACAGCTAAAAGTCAGTGGCGCGATTACTGTATGAAGGTCAAAGACTTGCCACAGTACGAAGCTGTTGCATTAAACACATCTGGTTCAACACCAAAAGATTTGTTTGAGGATGTTGCTGAAGAATTAGAGAAACAGTTTTTGGAAGATAAAGCACGGGTAAAGGATGCAATGAAGCAAGGGCAGATTGCAATGGTTTCCTCATGGACATTTGAAGAATTTAAGGCTGCTGTTGTGAAAGATATTGGTTCCCCATCAATTCCAGAGCTCAACTTAAAGCTTGCATATGAGGAGATATTAGAGAGAGCCAAGGAGAAGGAGGAAAAAGAAGCAAAGAAGCGTCAACGACTTGCTGATGACTTCACTAAGCTTTTGCACACATTCAAGGAAATAACTGCTTCTTCTAACTGGGAAGATTGCAAACACCTTTTTGAAGAGACCCAAGAATACAGATCAGTTGGGGACGAGAACTCTGGAAAGGAAGTTTTTGAGGAATATATTACAAGTTTACACGAGAAGGCCAAAGAGAAAGAACGCAAGCGTGAGGAGGAAAAGGTGTGGTTTTTACTGTCTGTTATATGCTGTGGCTATCATTATACTTCATTAGTTATTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005685 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006928 GO:0006996 GO:0007010 GO:0008150 GO:0008152 GO:0008360 GO:0008380 GO:0009987 GO:0010467 GO:0010564 GO:0016043 GO:0016070 GO:0016071 GO:0016363 GO:0016477 GO:0016592 GO:0016604 GO:0016607 GO:0022603 GO:0022604 GO:0030532 GO:0031974 GO:0031981 GO:0032465 GO:0032991 GO:0034399 GO:0034641 GO:0040011 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051302 GO:0051674 GO:0051726 GO:0065007 GO:0065008 GO:0070013 GO:0070064 GO:0071004 GO:0071010 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097525 GO:0120114 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

FF WW

Protein Analysis

869

Amino Acids

98.6

Weight (kDa)

5.62

Isoelectric Point (pI)

58.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	185 - 212	1.5e-09	WW domain
WW	PF00397	228 - 253	1e-07	WW domain
FF	PF01846	438 - 487	5.3e-17	FF domain
FF	PF01846	505 - 555	6.9e-13	FF domain
FF	PF01846	647 - 702	5.1e-09	FF domain
FF_PRPF40A	PF25432	710 - 769	7.2e-20	PRPF40A-like, FF domain
FF	PF01846	783 - 830	2.4e-06	FF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44910 AT1G44910
fragaria_vesca	FvH4_2g32680 FvH4_2g32680 FvH4_2g32680 FvH4_2g32680
malus_domestica	MD08G1078500.v1.1 MD15G1065300.v1.1
prunus_persica	Prupe.1G419400_v2.0.a1
pyrus_communis	pycom08g06490 pycom15g06180
rosa_chinensis	RchiOBHm_Chr6g0309991
rosa_laevigata	RLG00000010518
rosa_multiflora	Rmu_sc0000294.1_g000011
rosa_roxburghii	Rroxscaffold_7G00158970
rosa_rugosa	Rorug06G0376700 Rorug06G0376800
rosa_samantha	Rh6AG490400 Rh6BG499900 Rh6CG504800 Rh6DG490800
rosa_wichuraiana	Rw6G042690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	268, 415, 1060
Acc65I	GGTACC	1 cut(s)	46
AccB1I	GGYRCC	1 cut(s)	46
AccBSI	CCGCTC	1 cut(s)	1438
AccII	CGCG	1 cut(s)	2016
AciI	CCGC	5 cut(s)	323, 1347, 1438, 1843, 1927
AclWI	GGATC	2 cut(s)	506, 1000
AcsI	RAATTY	4 cut(s)	826, 1526, 1739, 2210
AcuI	CTGAAG	5 cut(s)	843, 891, 1245, 1725, 2133
AdeI	CACNNNGTG	1 cut(s)	1433
AfiI	CCNNNNNNNGG	3 cut(s)	413, 918, 1882
AjnI	CCWGG	8 cut(s)	169, 286, 916, 1006, 1338, 1370, 1461, 1857
AjuI	GAANNNNNNNTTGG	2 cut(s)	2117, 2149
Alw21I	GWGCWC	1 cut(s)	2262
Alw26I	GTCTC	3 cut(s)	387, 931, 2423
AlwI	GGATC	2 cut(s)	506, 1000
AlwNI	CAGNNNCTG	2 cut(s)	515, 1238
Ama87I	CYCGRG	2 cut(s)	928, 1518
AoxI	GGCC	5 cut(s)	158, 224, 893, 1465, 2511
ApaI	GGGCCC	1 cut(s)	897
ApeKI	GCWGC	8 cut(s)	26, 142, 410, 452, 475, 1700, 1979, 2219
ApoI	RAATTY	4 cut(s)	826, 1526, 1739, 2210
AseI	ATTAAT	1 cut(s)	1398
Asp718I	GGTACC	1 cut(s)	46
AspLEI	GCGC	1 cut(s)	2016
AspS9I	GGNCC	4 cut(s)	893, 894, 1368, 1465
AsuC2I	CCSGG	1 cut(s)	1946
AsuHPI	GGTGA	3 cut(s)	1072, 1445, 1780
AvaI	CYCGRG	2 cut(s)	928, 1518
AvaII	GGWCC	1 cut(s)	1368
BaeGI	GKGCMC	1 cut(s)	897
BanI	GGYRCC	1 cut(s)	46
BanII	GRGCYC	3 cut(s)	897, 1519, 2262
BauI	CACGAG	2 cut(s)	777, 2504
BbsI	GAAGAC	1 cut(s)	1011
Bbv12I	GWGCWC	1 cut(s)	2262
BbvCI	CCTCAGC	1 cut(s)	22
BbvI	GCAGC	8 cut(s)	13, 154, 397, 464, 487, 1687, 1966, 2206
BccI	CCATC	7 cut(s)	250, 575, 698, 959, 1181, 1569, 2254
BceAI	ACGGC	4 cut(s)	130, 193, 529, 1040
BcgI	CGANNNNNNTGC	2 cut(s)	2042, 2076
BciT130I	CCWGG	8 cut(s)	171, 288, 918, 1008, 1340, 1372, 1463, 1859
BciVI	GTATCC	1 cut(s)	159
BcnI	CCSGG	1 cut(s)	1946
BcoDI	GTCTC	3 cut(s)	387, 931, 2423
BfaI	CTAG	5 cut(s)	480, 612, 882, 1541, 1635
BfmI	CTRYAG	1 cut(s)	699
BfuAI	ACCTGC	3 cut(s)	268, 415, 1060
BfuI	GTATCC	1 cut(s)	159
BglII	AGATCT	1 cut(s)	1648
BisI	GCNGC	8 cut(s)	27, 143, 411, 453, 476, 1701, 1980, 2220
BlsI	GCNGC	8 cut(s)	28, 144, 412, 454, 477, 1702, 1981, 2221
BmcAI	AGTACT	2 cut(s)	610, 1099
Bme1390I	CCNGG	9 cut(s)	171, 288, 918, 1008, 1340, 1372, 1463, 1859, 1946
Bme18I	GGWCC	1 cut(s)	1368
BmeT110I	CYCGRG	2 cut(s)	928, 1518
BmgT120I	GGNCC	4 cut(s)	893, 894, 1368, 1465
BmiI	GGNNCC	3 cut(s)	48, 895, 2242
BmrFI	CCNGG	9 cut(s)	171, 288, 918, 1008, 1340, 1372, 1463, 1859, 1946
BmrI	ACTGGG	2 cut(s)	1439, 2410
BmsI	GCATC	7 cut(s)	273, 658, 680, 1169, 1808, 1896, 2149
BmuI	ACTGGG	2 cut(s)	1439, 2410
BpiI	GAAGAC	1 cut(s)	1011
BpmI	CTGGAG	5 cut(s)	418, 677, 897, 1122, 1766
Bpu10I	CCTNAGC	1 cut(s)	22
BpuEI	CTTGAG	5 cut(s)	369, 1149, 1185, 1697, 1898
BpuMI	CCSGG	1 cut(s)	1946
BsaI	GGTCTC	1 cut(s)	2423
BsaJI	CCNNGG	9 cut(s)	221, 422, 917, 1023, 1056, 1371, 1462, 1551, 2301
BsaXI	ACNNNNNCTCC	2 cut(s)	484, 514
Bsc4I	CCNNNNNNNGG	3 cut(s)	413, 918, 1882
Bse1I	ACTGG	9 cut(s)	227, 435, 621, 660, 935, 1105, 1302, 1434, 2405
Bse3DI	GCAATG	4 cut(s)	119, 1389, 2169, 2190
BseBI	CCWGG	8 cut(s)	171, 288, 918, 1008, 1340, 1372, 1463, 1859
BseDI	CCNNGG	9 cut(s)	221, 422, 917, 1023, 1056, 1371, 1462, 1551, 2301
BseGI	GGATG	4 cut(s)	432, 1954, 2110, 2164
BseLI	CCNNNNNNNGG	3 cut(s)	413, 918, 1882
BseMI	GCAATG	4 cut(s)	119, 1389, 2169, 2190
BseMII	CTCAG	4 cut(s)	29, 36, 44, 169
BseNI	ACTGG	9 cut(s)	227, 435, 621, 660, 935, 1105, 1302, 1434, 2405
BseRI	GAGGAG	7 cut(s)	776, 825, 834, 1280, 1907, 2297, 2552
BseSI	GKGCMC	1 cut(s)	897
BseXI	GCAGC	8 cut(s)	13, 154, 397, 464, 487, 1687, 1966, 2206
BseYI	CCCAGC	1 cut(s)	896
BsgI	GTGCAG	1 cut(s)	471
Bsh1236I	CGCG	1 cut(s)	2016
BshFI	GGCC	5 cut(s)	160, 226, 895, 1467, 2513
BshNI	GGYRCC	1 cut(s)	46
BsiHKAI	GWGCWC	1 cut(s)	2262
BsiHKCI	CYCGRG	2 cut(s)	928, 1518
BsiSI	CCGG	1 cut(s)	1945
BslFI	GGGAC	5 cut(s)	120, 224, 934, 1054, 2468
BslI	CCNNNNNNNGG	3 cut(s)	413, 918, 1882
BsmAI	GTCTC	3 cut(s)	387, 931, 2423
BsmFI	GGGAC	5 cut(s)	120, 224, 934, 1054, 2468
BsmI	GAATGC	2 cut(s)	297, 1325
BsnI	GGCC	5 cut(s)	160, 226, 895, 1467, 2513
Bso31I	GGTCTC	1 cut(s)	2423
BsoBI	CYCGRG	2 cut(s)	928, 1518
Bsp120I	GGGCCC	1 cut(s)	893
Bsp1286I	GDGCHC	3 cut(s)	897, 1519, 2262
Bsp1407I	TGTACA	1 cut(s)	1012
Bsp143I	GATC	7 cut(s)	511, 992, 1392, 1639, 1648, 1795, 2444
Bsp19I	CCATGG	1 cut(s)	1023
BspACI	CCGC	5 cut(s)	323, 1347, 1438, 1843, 1927
BspANI	GGCC	5 cut(s)	160, 226, 895, 1467, 2513
BspCNI	CTCAG	4 cut(s)	28, 35, 43, 168
BspFNI	CGCG	1 cut(s)	2016
BspLI	GGNNCC	3 cut(s)	48, 895, 2242
BspMAI	CTGCAG	1 cut(s)	703
BspMI	ACCTGC	3 cut(s)	268, 415, 1060
BspPI	GGATC	2 cut(s)	506, 1000
BspQI	GCTCTTC	1 cut(s)	1701
BspT107I	GGYRCC	1 cut(s)	46
BspTNI	GGTCTC	1 cut(s)	2423
BsrBI	CCGCTC	1 cut(s)	1438
BsrDI	GCAATG	4 cut(s)	119, 1389, 2169, 2190
BsrGI	TGTACA	1 cut(s)	1012
BsrI	ACTGG	9 cut(s)	227, 435, 621, 660, 935, 1105, 1302, 1434, 2405
BssECI	CCNNGG	9 cut(s)	221, 422, 917, 1023, 1056, 1371, 1462, 1551, 2301
BssMI	GATC	7 cut(s)	511, 992, 1392, 1639, 1648, 1795, 2444
BssSI	CACGAG	2 cut(s)	777, 2504
BssT1I	CCWWGG	4 cut(s)	422, 1023, 1551, 2301
Bst2BI	CACGAG	2 cut(s)	777, 2504
Bst2UI	CCWGG	8 cut(s)	171, 288, 918, 1008, 1340, 1372, 1463, 1859
Bst6I	CTCTTC	5 cut(s)	1485, 1539, 1701, 1884, 2421
BstAPI	GCANNNNNTGC	1 cut(s)	1322
BstAUI	TGTACA	1 cut(s)	1012
BstDEI	CTNAG	6 cut(s)	15, 22, 30, 155, 1801, 2358
BstDSI	CCRYGG	2 cut(s)	1023, 1056
BstENI	CCTNNNNNAGG	1 cut(s)	1880
BstF5I	GGATG	4 cut(s)	432, 1954, 2110, 2164
BstFNI	CGCG	1 cut(s)	2016
BstHHI	GCGC	1 cut(s)	2016
BstKTI	GATC	7 cut(s)	514, 995, 1395, 1642, 1651, 1798, 2447
BstMAI	GTCTC	3 cut(s)	387, 931, 2423
BstMBI	GATC	7 cut(s)	511, 992, 1392, 1639, 1648, 1795, 2444
BstMWI	GCNNNNNNNGC	7 cut(s)	166, 292, 407, 484, 848, 1322, 1504
BstNI	CCWGG	8 cut(s)	171, 288, 918, 1008, 1340, 1372, 1463, 1859
BstNSI	RCATGY	3 cut(s)	401, 875, 1979
BstSCI	CCNGG	9 cut(s)	169, 286, 916, 1006, 1338, 1370, 1461, 1857, 1944
BstSFI	CTRYAG	1 cut(s)	699
BstSLI	GKGCMC	1 cut(s)	897
BstUI	CGCG	1 cut(s)	2016
BstV1I	GCAGC	8 cut(s)	13, 154, 397, 464, 487, 1687, 1966, 2206
BstV2I	GAAGAC	1 cut(s)	1011
BstX2I	RGATCY	2 cut(s)	511, 1648
BstYI	RGATCY	2 cut(s)	511, 1648
BsuI	GTATCC	1 cut(s)	159
BsuRI	GGCC	5 cut(s)	160, 226, 895, 1467, 2513
BtgI	CCRYGG	2 cut(s)	1023, 1056
BtsCI	GGATG	4 cut(s)	432, 1954, 2110, 2164
BtsI	GCAGTG	1 cut(s)	1073
BtsIMutI	CAGTG	4 cut(s)	653, 1073, 1427, 2015
BveI	ACCTGC	3 cut(s)	268, 415, 1060
CaiI	CAGNNNCTG	2 cut(s)	515, 1238
CfoI	GCGC	1 cut(s)	2016
Cfr13I	GGNCC	4 cut(s)	893, 894, 1368, 1465
CseI	GACGC	2 cut(s)	1814, 2321
CspCI	CAANNNNNGTGG	2 cut(s)	1346, 1381
CviAII	CATG	7 cut(s)	176, 398, 839, 872, 1024, 1976, 2196
DdeI	CTNAG	6 cut(s)	15, 22, 30, 155, 1801, 2358
DpnI	GATC	7 cut(s)	513, 994, 1394, 1641, 1650, 1797, 2446
DpnII	GATC	7 cut(s)	511, 992, 1392, 1639, 1648, 1795, 2444
DraIII	CACNNNGTG	1 cut(s)	1433
Eam1104I	CTCTTC	5 cut(s)	1485, 1539, 1701, 1884, 2421
EarI	CTCTTC	5 cut(s)	1485, 1539, 1701, 1884, 2421
EciI	GGCGGA	1 cut(s)	1942
Ecl136II	GAGCTC	1 cut(s)	2260
Eco130I	CCWWGG	4 cut(s)	422, 1023, 1551, 2301
Eco24I	GRGCYC	3 cut(s)	897, 1519, 2262
Eco31I	GGTCTC	1 cut(s)	2423
Eco47I	GGWCC	1 cut(s)	1368
Eco53kI	GAGCTC	1 cut(s)	2260
Eco57I	CTGAAG	5 cut(s)	843, 891, 1245, 1725, 2133
Eco88I	CYCGRG	2 cut(s)	928, 1518
EcoICRI	GAGCTC	1 cut(s)	2260
EcoNI	CCTNNNNNAGG	1 cut(s)	1880
EcoRII	CCWGG	8 cut(s)	169, 286, 916, 1006, 1338, 1370, 1461, 1857
EcoT14I	CCWWGG	4 cut(s)	422, 1023, 1551, 2301
EcoT22I	ATGCAT	2 cut(s)	673, 1327
EcoT38I	GRGCYC	3 cut(s)	897, 1519, 2262
ErhI	CCWWGG	4 cut(s)	422, 1023, 1551, 2301
FaeI	CATG	7 cut(s)	179, 401, 842, 875, 1027, 1979, 2199
FalI	AAGNNNNNCTT	2 cut(s)	487, 519
FaqI	GGGAC	5 cut(s)	120, 224, 934, 1054, 2468
FatI	CATG	7 cut(s)	175, 397, 838, 871, 1023, 1975, 2195
FauNDI	CATATG	2 cut(s)	570, 2278
Fnu4HI	GCNGC	8 cut(s)	27, 143, 411, 453, 476, 1701, 1980, 2220
FokI	GGATG	4 cut(s)	439, 1961, 2117, 2171
FriOI	GRGCYC	3 cut(s)	897, 1519, 2262
Fsp4HI	GCNGC	8 cut(s)	27, 143, 411, 453, 476, 1701, 1980, 2220
FspBI	CTAG	5 cut(s)	480, 612, 882, 1541, 1635
GlaI	GCGC	1 cut(s)	2015
GluI	GCNGC	8 cut(s)	27, 143, 411, 453, 476, 1701, 1980, 2220
GsaI	CCCAGC	1 cut(s)	900
GsuI	CTGGAG	5 cut(s)	418, 677, 897, 1122, 1766
HaeIII	GGCC	5 cut(s)	160, 226, 895, 1467, 2513
HapII	CCGG	1 cut(s)	1945
HgaI	GACGC	2 cut(s)	1814, 2321
HhaI	GCGC	1 cut(s)	2016
Hin1II	CATG	7 cut(s)	179, 401, 842, 875, 1027, 1979, 2199
Hin6I	GCGC	1 cut(s)	2014
HinP1I	GCGC	1 cut(s)	2014
HincII	GTYRAC	3 cut(s)	1123, 1249, 2336
HindII	GTYRAC	3 cut(s)	1123, 1249, 2336
HindIII	AAGCTT	3 cut(s)	1835, 2270, 2360
HinfI	GANTC	8 cut(s)	649, 740, 832, 931, 1255, 1343, 1547, 1748
HpaII	CCGG	1 cut(s)	1945
HphI	GGTGA	3 cut(s)	1072, 1445, 1780
Hpy166II	GTNNAC	7 cut(s)	1013, 1123, 1249, 1357, 1771, 2336, 2501
Hpy188I	TCNGA	6 cut(s)	282, 556, 820, 1264, 1363, 1729
Hpy8I	GTNNAC	7 cut(s)	1013, 1123, 1249, 1357, 1771, 2336, 2501
HpyCH4IV	ACGT	1 cut(s)	1251
HpyF10VI	GCNNNNNNNGC	7 cut(s)	166, 292, 407, 484, 848, 1322, 1504
HpyF3I	CTNAG	6 cut(s)	15, 22, 30, 155, 1801, 2358
HpySE526I	ACGT	1 cut(s)	1251
Hsp92II	CATG	7 cut(s)	179, 401, 842, 875, 1027, 1979, 2199
HspAI	GCGC	1 cut(s)	2014
KpnI	GGTACC	1 cut(s)	50
Kzo9I	GATC	7 cut(s)	511, 992, 1392, 1639, 1648, 1795, 2444
LguI	GCTCTTC	1 cut(s)	1701
LmnI	GCTCC	8 cut(s)	88, 847, 878, 1009, 1190, 1375, 1578, 1894
Lsp1109I	GCAGC	8 cut(s)	13, 154, 397, 464, 487, 1687, 1966, 2206
LweI	GCATC	7 cut(s)	273, 658, 680, 1169, 1808, 1896, 2149
MaeI	CTAG	5 cut(s)	480, 612, 882, 1541, 1635
MaeII	ACGT	1 cut(s)	1251
MaeIII	GTNAC	7 cut(s)	229, 460, 730, 943, 1252, 1588, 1871
MalI	GATC	7 cut(s)	513, 994, 1394, 1641, 1650, 1797, 2446
MbiI	CCGCTC	1 cut(s)	1438
MboI	GATC	7 cut(s)	511, 992, 1392, 1639, 1648, 1795, 2444
MfeI	CAATTG	3 cut(s)	1182, 1275, 1919
MflI	RGATCY	2 cut(s)	511, 1648
MhlI	GDGCHC	3 cut(s)	897, 1519, 2262
MlyI	GAGTC	6 cut(s)	643, 826, 940, 1249, 1556, 1757
MmeI	TCCRAC	2 cut(s)	1021, 1461
Mph1103I	ATGCAT	2 cut(s)	673, 1327
MslI	CAYNNNNRTG	1 cut(s)	447
MspA1I	CMGCKG	2 cut(s)	26, 863
MspI	CCGG	1 cut(s)	1945
MspR9I	CCNGG	9 cut(s)	171, 288, 918, 1008, 1340, 1372, 1463, 1859, 1946
MunI	CAATTG	3 cut(s)	1182, 1275, 1919
Mva1269I	GAATGC	2 cut(s)	297, 1325
MvaI	CCWGG	8 cut(s)	171, 288, 918, 1008, 1340, 1372, 1463, 1859
MvnI	CGCG	1 cut(s)	2016
MwoI	GCNNNNNNNGC	7 cut(s)	166, 292, 407, 484, 848, 1322, 1504
NciI	CCSGG	1 cut(s)	1946
NcoI	CCATGG	1 cut(s)	1023
NdeI	CATATG	2 cut(s)	570, 2278
NdeII	GATC	7 cut(s)	511, 992, 1392, 1639, 1648, 1795, 2444
NlaIII	CATG	7 cut(s)	179, 401, 842, 875, 1027, 1979, 2199
NlaIV	GGNNCC	3 cut(s)	48, 895, 2242
NmeAIII	GCCGAG	1 cut(s)	246
NmuCI	GTSAC	2 cut(s)	1252, 1871
NsiI	ATGCAT	2 cut(s)	673, 1327
NspI	RCATGY	3 cut(s)	401, 875, 1979
PaeR7I	CTCGAG	1 cut(s)	928
PciSI	GCTCTTC	1 cut(s)	1701
PctI	GAATGC	2 cut(s)	297, 1325
PfeI	GAWTC	2 cut(s)	740, 1343
PflFI	GACNNNGTC	1 cut(s)	111
PfoI	TCCNGGA	2 cut(s)	1338, 1857
PkrI	GCNGC	8 cut(s)	28, 144, 412, 454, 477, 1702, 1981, 2221
PleI	GAGTC	6 cut(s)	643, 826, 939, 1249, 1555, 1756
PpsI	GAGTC	6 cut(s)	643, 826, 939, 1249, 1555, 1756
PshBI	ATTAAT	1 cut(s)	1398
Psp124BI	GAGCTC	1 cut(s)	2262
Psp6I	CCWGG	8 cut(s)	169, 286, 916, 1006, 1338, 1370, 1461, 1857
PspFI	CCCAGC	1 cut(s)	896
PspGI	CCWGG	8 cut(s)	169, 286, 916, 1006, 1338, 1370, 1461, 1857
PspN4I	GGNNCC	3 cut(s)	48, 895, 2242
PspOMI	GGGCCC	1 cut(s)	893
PspPI	GGNCC	4 cut(s)	893, 894, 1368, 1465
PstI	CTGCAG	1 cut(s)	703
PstNI	CAGNNNCTG	2 cut(s)	515, 1238
PsuI	RGATCY	2 cut(s)	511, 1648
PsyI	GACNNNGTC	1 cut(s)	111
PvuII	CAGCTG	2 cut(s)	26, 863
RseI	CAYNNNNRTG	1 cut(s)	447
SacI	GAGCTC	1 cut(s)	2262
SapI	GCTCTTC	1 cut(s)	1701
SatI	GCNGC	8 cut(s)	27, 143, 411, 453, 476, 1701, 1980, 2220
Sau3AI	GATC	7 cut(s)	511, 992, 1392, 1639, 1648, 1795, 2444
Sau96I	GGNCC	4 cut(s)	893, 894, 1368, 1465
ScaI	AGTACT	2 cut(s)	610, 1099
SchI	GAGTC	6 cut(s)	643, 826, 940, 1249, 1556, 1757
ScrFI	CCNGG	9 cut(s)	171, 288, 918, 1008, 1340, 1372, 1463, 1859, 1946
SduI	GDGCHC	3 cut(s)	897, 1519, 2262
SfaNI	GCATC	7 cut(s)	273, 658, 680, 1169, 1808, 1896, 2149
SfcI	CTRYAG	1 cut(s)	699
Sfr274I	CTCGAG	1 cut(s)	928
SinI	GGWCC	1 cut(s)	1368
SlaI	CTCGAG	1 cut(s)	928
SmiMI	CAYNNNNRTG	1 cut(s)	447
SmlI	CTYRAG	6 cut(s)	384, 928, 1164, 1200, 1676, 1877
SmoI	CTYRAG	6 cut(s)	384, 928, 1164, 1200, 1676, 1877
SsiI	CCGC	5 cut(s)	323, 1347, 1438, 1843, 1927
SspI	AATATT	1 cut(s)	128
SspMI	CTAG	5 cut(s)	480, 612, 882, 1541, 1635
SstI	GAGCTC	1 cut(s)	2262
StyD4I	CCNGG	9 cut(s)	169, 286, 916, 1006, 1338, 1370, 1461, 1857, 1944
StyI	CCWWGG	4 cut(s)	422, 1023, 1551, 2301
TaiI	ACGT	1 cut(s)	1254
TaqI	TCGA	2 cut(s)	37, 929
TatI	WGTACW	4 cut(s)	608, 1012, 1097, 1731
TfiI	GAWTC	2 cut(s)	740, 1343
TscAI	CASTG	4 cut(s)	660, 1080, 1434, 2015
TseFI	GTSAC	2 cut(s)	1252, 1871
TseI	GCWGC	8 cut(s)	26, 142, 410, 452, 475, 1700, 1979, 2219
Tsp45I	GTSAC	2 cut(s)	1252, 1871
TspRI	CASTG	4 cut(s)	660, 1080, 1434, 2015
Tth111I	GACNNNGTC	1 cut(s)	111
VpaK11BI	GGWCC	1 cut(s)	1368
VspI	ATTAAT	1 cut(s)	1398
XagI	CCTNNNNNAGG	1 cut(s)	1880
XapI	RAATTY	4 cut(s)	826, 1526, 1739, 2210
XceI	RCATGY	3 cut(s)	401, 875, 1979
XhoI	CTCGAG	1 cut(s)	928
XspI	CTAG	5 cut(s)	480, 612, 882, 1541, 1635
ZrmI	AGTACT	2 cut(s)	610, 1099
Zsp2I	ATGCAT	2 cut(s)	673, 1327

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.