Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009577

Physical Location & Seq

Genomic Coordinates Forward (+)

4546115 .. 4557138

Gene Structure 11024 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1065300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGGCGAATAACCCTCAATCCTCAGCTGCGCAGCCATTTCGGCTTCCCCCAGTTGCTCCCATGGGTCCTCAGAGTTTTGGTTCATCTCCTCTTCAATACCGACCAGTGGTTCCAACACAGCAAGGACAGCAATTTATTTCACCAGCTCCCCAACAATTTCAGCCTGTGGGACAAGGTATTCCTTCTCCAAATGTTGGGATGCCTGCTAGTCAAAGTCAGCAATTACAATATTCTCAACCAATGCAACCGTATCCTCTCAGGCCAAGTCAGCCTGGTCATCCTCACTCGTCACAGGCGTTGCCTATGCAATACATGCAAACGAGGCCTATTACATCTATCCCATCACAGTCTCAGCAACCTGTCCCACCTTTTAATAACCAAATGCCTGGAATGCCTTATTCTTCCTCATATGTGTTCACTCCGCCTACGTATGCCCAGCCACAAAATAATGTCAATTCACAGTTTCAGCCGATGTCTCAAATGCAAGCACATGTTGTATCTGCTACAGGACAGCCTTGGGTGTCCTCTGGTAATCAGGGTGTAGCAGTTCCTTTTCCAGTACAGCAGTCTAGCCAACAATCTTCAAGCACTCCTTCCACAGATTCTGCTGTAAATGTTTCTAGCCAAGCACAGCAATCTTCTTCGGATTGGCAAGAGCATTTGGCGGTTGATGGAAGAAGATATTATTTCAACAGAAGGACAAGACAATCTAATTGGGAGAAGCCTTTAGAATTGATGACACCGATGGAGAGGGCTGATGCATCAACTGTTTGGAAGGAGTATACATCTGCAGATGGGAAAAAGTATTATTACAACAAGGTTACAAGGGAGTCTAAGTGGACAATTCCAGAGGAGTTGAAGTTGGCTCGTGAACAGGCTCAAAGAGAACTAATCCAAGGAACACATTCAGAATTGAATTTGACTTCTCATACCCCTCCTGCTGTTGCTTCAGCTGAAACACCTATGGGAGCTAGTTCAGTTGGCCCCAGCACTTCTTCAGCCCTACCTGTGATGGTCTCGAGCCCTGTTGCAGTTACACCTGTGTCTTCCTTTTCTAATCCTTCTGCTATCACACCTACTGGATCATCAGTTGCTTCTGGTGCACAGCCTTCCATAACTGGTGCCGTTGGCAGTCAACCTTCTGCAGTTACTGTGAGTCCACTGCCCGCTTCTGTTTCAGGGAGTTCCGGAGTTCCTCCTACTTCGGTCAATACCACTACAAATTCACTGAGTACTTATGAAACTGTAGCATCTCAAGATATTTCAAGTTCAGCTGATGGAACTTTGACTCAAGATATTGAGGAAGCCAAAAGAGGTATGGCAGTTGCTGGAAAAGTCAATGTGACTCCTTCAGAGGAGAAAACATTTGATGACGAACCATTGGTGTATGCCAATAAGCAGGAGGCAAAAACTGCATTCAAATCACTTCTGGAATCTGCAAATGTTCAGTCCGATTGGACTTGGGAGCAGACAATGAGGGAGATCATTACTGACAAAAGATATGGCGCATTAAGAACTCTAGGTGAGAGGAAGCAAGCATTTAATGAGTACTTGGGTCAAAGGAAAAAACTGGAGAATGAAGAGAGGCGCGTGAGGCAGAAAAAAGCTCGGGAAGAATTCACAAAAATGTTGGAAGAGTCCAAGGAACTTACATCAGCAACTAAATGGAGCAAAGCTGTGAGTATGTTTGAAAATGATGGACGATTCAAGGCTGTAGAAAGAGGTAGAGACCAGGAAGATCTTTATGAGAGCTACATTGTAGAACTCGAAAGGAAGGAAAAAGAAAAGGCAGCTGAAGACCATAAGCAGAATATAGTAGAGTACCGGAAATTTCTGGAATCTTGTGACTTTATCAAGGTGAACAGTCAGTGGCGGAAAGTTCAAGATCGCTTGGAGGATGATGAGAGATGCTTGCGCCTTGAAAAGCTTGCCAGGTTGCTCATTTTCCAGGACTATATTCGTGACCTCGAGAAGGTGGAAGAGGAGCAAAAGAAGTTACAGAAGGAACAATTGAGGCGGGTAGAAAGGAAAAACCGCGATGAGTTCCGCAAGCTGATGGAAGAACATGTTGCCGATGGCACTCTTACAGCTAAAACTCTCTGGCGTGATTACTGTTTGAAGGTTAAAGACTTGCCTCCTTATGAAGCTGTTGCATTGAACACCTCTGGTTCAACACCAAAAGAATTGTTTGATGATGTTTTTGAAGAATTAGAGAAACAGTATCATGACGATAAAGCTAGGATAAAGGATGCAATGAAGTCAGGGAAGGTTGCCATGGCATCCACATTGGCGTTTGAAGAGTTTAAGGTTCCTATTCTGGAAGAAATTGGTTCTCCACCAATATCAGACATTAACTTCAAGCTTGTGTACGAGGAATTATTAGAGAGAGCCAAGGAGAAGGAGGAGAAAGAGGCAAAGAAGCGTCAACGACTTGCTGATGATTTCAATAAACTTTTGCACACAATCAAGGAGATAACGGCTTCTTCTAATTGGGAAGATAGCAAACAGCTTTTTGAAGAAACCCAAGAATACAGATCAATTGGGGAAGATAGCGTTAGTAGGGAAATTTTTGAGGAATACATTACAAACTTACAGGAGAGGGCTAAAGAGAAGGAACGCAAGCGTGAGGAGGAAAAGGCCAAAAAAGAGAGAGAAAGAGAGGAGAAAGAGAAGCGGAAGGATAAAGAAAGAAAGGAGAAGGAAAGAGAACGTGAAAAAGAAAAGGGAAGGGAACGATCTAAGAAGGATGAAACAGATAGTGAAAATGTAGACATAACTGATACTCATGGGCATAAAGAAGATAAGAAGAGGGAGAAAGACAAAGATAGAAAGCACCGGAAGCGGCATCAAAGTTCTGTCGATGATGTAGGTTCTGATAAGGAGGAGAAAGAGGAGTCTAAGAAACACAGACATAGTAGTGACCGCAAAAGATCAAGAAAACATACACCTGAATCTGACAGTGAAAGTCGGCATAGAAGGCATAGGAGAGAGCATCGGGATGGTTCCCGTCGAATTGGTGGGCATGACGATCTTGAAGATGGGGAGCTTGGTGAAGATGGGGAAATTAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005685 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006928 GO:0006996 GO:0007010 GO:0008150 GO:0008152 GO:0008360 GO:0008380 GO:0009987 GO:0010467 GO:0010564 GO:0016043 GO:0016070 GO:0016071 GO:0016363 GO:0016477 GO:0016592 GO:0016604 GO:0016607 GO:0022603 GO:0022604 GO:0030532 GO:0031974 GO:0031981 GO:0032465 GO:0032991 GO:0034399 GO:0034641 GO:0040011 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051302 GO:0051674 GO:0051726 GO:0065007 GO:0065008 GO:0070013 GO:0070064 GO:0071004 GO:0071010 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097525 GO:0120114 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

FF WW

Protein Analysis

1024

Amino Acids

116.66

Weight (kDa)

6.4

Isoelectric Point (pI)

60.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	215 - 242	3.1e-07	WW domain
WW	PF00397	258 - 283	1.2e-07	WW domain
FF	PF01846	468 - 517	9.2e-16	FF domain
FF	PF01846	535 - 585	1.7e-12	FF domain
FF	PF01846	677 - 732	2.7e-06	FF domain
FF_PRPF40A	PF25432	740 - 799	1e-19	PRPF40A-like, FF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44910 AT1G44910
fragaria_vesca	FvH4_2g32680 FvH4_2g32680 FvH4_2g32680 FvH4_2g32680
malus_domestica	MD08G1078500.v1.1 MD15G1065300.v1.1
prunus_persica	Prupe.1G419400_v2.0.a1
pyrus_communis	pycom08g06490 pycom15g06180
rosa_chinensis	RchiOBHm_Chr6g0309991
rosa_laevigata	RLG00000010518
rosa_multiflora	Rmu_sc0000294.1_g000011
rosa_roxburghii	Rroxscaffold_7G00158970
rosa_rugosa	Rorug06G0376700 Rorug06G0376800
rosa_samantha	Rh6AG490400 Rh6BG499900 Rh6CG504800 Rh6DG490800
rosa_wichuraiana	Rw6G042690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	30
AccB1I	GGYRCC	1 cut(s)	1121
AccB7I	CCANNNNNTGG	1 cut(s)	194
AccI	GTMKAC	2 cut(s)	782, 2769
AccII	CGCG	2 cut(s)	1590, 2037
AccIII	TCCGGA	1 cut(s)	1187
AclWI	GGATC	1 cut(s)	1090
AcsI	RAATTY	5 cut(s)	916, 1222, 1616, 1829, 2565
AcuI	CTGAAG	4 cut(s)	933, 981, 1335, 1815
AfaI	GTAC	5 cut(s)	561, 1234, 1550, 1823, 2369
AfiI	CCNNNNNNNGG	3 cut(s)	106, 194, 1972
AflIII	ACRYGT	2 cut(s)	490, 2065
AjnI	CCWGG	5 cut(s)	271, 385, 1731, 1931, 1947
Alw21I	GWGCWC	1 cut(s)	1105
Alw26I	GTCTC	4 cut(s)	354, 480, 1021, 1722
Alw44I	GTGCAC	1 cut(s)	1101
AlwI	GGATC	1 cut(s)	1090
AlwNI	CAGNNNCTG	3 cut(s)	605, 1151, 1328
Ama87I	CYCGRG	3 cut(s)	1018, 1608, 1967
Aor13HI	TCCGGA	1 cut(s)	1187
AoxI	GGCC	4 cut(s)	260, 323, 982, 2637
ApaLI	GTGCAC	1 cut(s)	1101
ApeKI	GCWGC	3 cut(s)	26, 31, 1790
ApoI	RAATTY	5 cut(s)	916, 1222, 1616, 1829, 2565
ArsI	GACNNNNNNTTYG	2 cut(s)	1433, 1465
Asp700I	GAANNNNTTC	1 cut(s)	904
AspLEI	GCGC	4 cut(s)	31, 1508, 1590, 1917
AspS9I	GGNCC	2 cut(s)	65, 983
AsuHPI	GGTGA	4 cut(s)	132, 1535, 1870, 3062
AvaI	CYCGRG	3 cut(s)	1018, 1608, 1967
AvaII	GGWCC	1 cut(s)	65
BaeGI	GKGCMC	1 cut(s)	1105
BanI	GGYRCC	1 cut(s)	1121
BanII	GRGCYC	1 cut(s)	1025
BauI	CACGAG	1 cut(s)	867
BbsI	GAAGAC	2 cut(s)	1038, 1803
Bbv12I	GWGCWC	1 cut(s)	1105
BbvCI	CCTCAGC	1 cut(s)	22
BbvI	GCAGC	3 cut(s)	13, 43, 1802
BceAI	ACGGC	2 cut(s)	1109, 2493
BcgI	CGANNNNNNTGC	2 cut(s)	20, 54
BciT130I	CCWGG	5 cut(s)	273, 387, 1733, 1933, 1949
BciVI	GTATCC	1 cut(s)	261
BcoDI	GTCTC	4 cut(s)	354, 480, 1021, 1722
BfaI	CTAG	6 cut(s)	207, 570, 621, 972, 1520, 2238
BfmI	CTRYAG	5 cut(s)	504, 789, 1143, 1245, 1713
BfuI	GTATCC	1 cut(s)	261
BglI	GCCNNNNNGGC	1 cut(s)	40
BglII	AGATCT	1 cut(s)	1738
BisI	GCNGC	4 cut(s)	27, 32, 1791, 2843
BlsI	GCNGC	4 cut(s)	28, 33, 1792, 2844
BmcAI	AGTACT	2 cut(s)	1234, 1550
Bme1390I	CCNGG	5 cut(s)	273, 387, 1733, 1933, 1949
Bme18I	GGWCC	1 cut(s)	65
BmeT110I	CYCGRG	3 cut(s)	1018, 1608, 1967
BmgT120I	GGNCC	2 cut(s)	65, 983
BmiI	GGNNCC	6 cut(s)	66, 111, 985, 1123, 2310, 3004
BmrFI	CCNGG	5 cut(s)	273, 387, 1733, 1933, 1949
BmrI	ACTGGG	1 cut(s)	44
BmsI	GCATC	9 cut(s)	189, 748, 770, 1259, 1898, 2239, 2288, 2854, 3001
BmuI	ACTGGG	1 cut(s)	44
BpiI	GAAGAC	2 cut(s)	1038, 1803
BpmI	CTGGAG	1 cut(s)	1592
Bpu10I	CCTNAGC	1 cut(s)	22
BpuEI	CTTGAG	2 cut(s)	1239, 1275
BsaAI	YACGTR	1 cut(s)	429
BsaI	GGTCTC	2 cut(s)	1021, 1722
BsaJI	CCNNGG	6 cut(s)	60, 515, 895, 1641, 2274, 2391
BsaWI	WCCGGW	3 cut(s)	1187, 1824, 2835
BsaXI	ACNNNNNCTCC	4 cut(s)	821, 845, 851, 875
Bsc4I	CCNNNNNNNGG	3 cut(s)	106, 194, 1972
Bse1I	ACTGG	6 cut(s)	50, 104, 557, 1084, 1123, 1575
Bse3DI	GCAATG	1 cut(s)	2259
BseAI	TCCGGA	1 cut(s)	1187
BseBI	CCWGG	5 cut(s)	273, 387, 1733, 1933, 1949
BseDI	CCNNGG	6 cut(s)	60, 515, 895, 1641, 2274, 2391
BseGI	GGATG	7 cut(s)	204, 277, 1903, 2254, 2279, 2752, 3004
BseLI	CCNNNNNNNGG	3 cut(s)	106, 194, 1972
BseMI	GCAATG	1 cut(s)	2259
BseMII	CTCAG	5 cut(s)	36, 83, 271, 365, 1220
BseNI	ACTGG	6 cut(s)	50, 104, 557, 1084, 1123, 1575
BseRI	GAGGAG	9 cut(s)	78, 866, 1370, 1997, 2417, 2642, 2675, 2897, 2906
BseSI	GKGCMC	1 cut(s)	1105
BseXI	GCAGC	3 cut(s)	13, 43, 1802
BseYI	CCCAGC	2 cut(s)	435, 986
Bsh1236I	CGCG	2 cut(s)	1590, 2037
BshFI	GGCC	4 cut(s)	262, 325, 984, 2639
BshNI	GGYRCC	1 cut(s)	1121
BsiHKAI	GWGCWC	1 cut(s)	1105
BsiHKCI	CYCGRG	3 cut(s)	1018, 1608, 1967
BsiSI	CCGG	3 cut(s)	1188, 1825, 2836
BslFI	GGGAC	2 cut(s)	183, 347
BslI	CCNNNNNNNGG	3 cut(s)	106, 194, 1972
BsmAI	GTCTC	4 cut(s)	354, 480, 1021, 1722
BsmFI	GGGAC	2 cut(s)	183, 347
BsmI	GAATGC	2 cut(s)	396, 1415
BsnI	GGCC	4 cut(s)	262, 325, 984, 2639
Bso31I	GGTCTC	2 cut(s)	1021, 1722
BsoBI	CYCGRG	3 cut(s)	1018, 1608, 1967
Bsp1286I	GDGCHC	2 cut(s)	1025, 1105
Bsp13I	TCCGGA	1 cut(s)	1187
Bsp143I	GATC	8 cut(s)	1082, 1482, 1738, 1885, 2534, 2735, 2930, 3028
Bsp19I	CCATGG	2 cut(s)	60, 2274
BspANI	GGCC	4 cut(s)	262, 325, 984, 2639
BspCNI	CTCAG	5 cut(s)	35, 82, 270, 364, 1221
BspEI	TCCGGA	1 cut(s)	1187
BspFNI	CGCG	2 cut(s)	1590, 2037
BspHI	TCATGA	1 cut(s)	2224
BspLI	GGNNCC	6 cut(s)	66, 111, 985, 1123, 2310, 3004
BspMAI	CTGCAG	2 cut(s)	793, 1147
BspPI	GGATC	1 cut(s)	1090
BspT107I	GGYRCC	1 cut(s)	1121
BspTNI	GGTCTC	2 cut(s)	1021, 1722
BsrDI	GCAATG	1 cut(s)	2259
BsrI	ACTGG	6 cut(s)	50, 104, 557, 1084, 1123, 1575
BssECI	CCNNGG	6 cut(s)	60, 515, 895, 1641, 2274, 2391
BssMI	GATC	8 cut(s)	1082, 1482, 1738, 1885, 2534, 2735, 2930, 3028
BssNAI	GTATAC	1 cut(s)	783
BssSI	CACGAG	1 cut(s)	867
BssT1I	CCWWGG	6 cut(s)	60, 515, 895, 1641, 2274, 2391
Bst1107I	GTATAC	1 cut(s)	783
Bst2BI	CACGAG	1 cut(s)	867
Bst2UI	CCWGG	5 cut(s)	273, 387, 1733, 1933, 1949
Bst6I	CTCTTC	6 cut(s)	96, 1575, 1629, 1974, 2292, 2801
BstAPI	GCANNNNNTGC	2 cut(s)	313, 1412
BstBAI	YACGTR	1 cut(s)	429
BstC8I	GCNNGC	8 cut(s)	204, 486, 1167, 1536, 1913, 1929, 2051, 2621
BstDEI	CTNAG	8 cut(s)	22, 69, 257, 351, 834, 1229, 2739, 2898
BstDSI	CCRYGG	2 cut(s)	60, 2274
BstENI	CCTNNNNNAGG	1 cut(s)	1970
BstF5I	GGATG	7 cut(s)	204, 277, 1903, 2254, 2279, 2752, 3004
BstFNI	CGCG	2 cut(s)	1590, 2037
BstHHI	GCGC	4 cut(s)	31, 1508, 1590, 1917
BstKTI	GATC	8 cut(s)	1085, 1485, 1741, 1888, 2537, 2738, 2933, 3031
BstMAI	GTCTC	4 cut(s)	354, 480, 1021, 1722
BstMBI	GATC	8 cut(s)	1082, 1482, 1738, 1885, 2534, 2735, 2930, 3028
BstNI	CCWGG	5 cut(s)	273, 387, 1733, 1933, 1949
BstNSI	RCATGY	3 cut(s)	316, 494, 2069
BstSCI	CCNGG	5 cut(s)	271, 385, 1731, 1931, 1947
BstSFI	CTRYAG	5 cut(s)	504, 789, 1143, 1245, 1713
BstSLI	GKGCMC	1 cut(s)	1105
BstSNI	TACGTA	1 cut(s)	429
BstUI	CGCG	2 cut(s)	1590, 2037
BstV1I	GCAGC	3 cut(s)	13, 43, 1802
BstV2I	GAAGAC	2 cut(s)	1038, 1803
BstX2I	RGATCY	1 cut(s)	1738
BstXI	CCANNNNNNTGG	1 cut(s)	386
BstYI	RGATCY	1 cut(s)	1738
BstZ17I	GTATAC	1 cut(s)	783
BsuI	GTATCC	1 cut(s)	261
BsuRI	GGCC	4 cut(s)	262, 325, 984, 2639
BtgI	CCRYGG	2 cut(s)	60, 2274
BtgZI	GCGATG	1 cut(s)	2052
BtsCI	GGATG	7 cut(s)	204, 277, 1903, 2254, 2279, 2752, 3004
BtsI	GCAGTG	1 cut(s)	1160
BtsIMutI	CAGTG	5 cut(s)	111, 1160, 1226, 1874, 2965
Cac8I	GCNNGC	8 cut(s)	204, 486, 1167, 1536, 1913, 1929, 2051, 2621
CaiI	CAGNNNCTG	3 cut(s)	605, 1151, 1328
CciI	TCATGA	1 cut(s)	2224
CfoI	GCGC	4 cut(s)	31, 1508, 1590, 1917
Cfr13I	GGNCC	2 cut(s)	65, 983
CseI	GACGC	1 cut(s)	2411
Csp6I	GTAC	5 cut(s)	560, 1233, 1549, 1822, 2368
CviAII	CATG	8 cut(s)	61, 313, 491, 2066, 2225, 2275, 2786, 3023
CviQI	GTAC	5 cut(s)	560, 1233, 1549, 1822, 2368
DdeI	CTNAG	8 cut(s)	22, 69, 257, 351, 834, 1229, 2739, 2898
DpnI	GATC	8 cut(s)	1084, 1484, 1740, 1887, 2536, 2737, 2932, 3030
DpnII	GATC	8 cut(s)	1082, 1482, 1738, 1885, 2534, 2735, 2930, 3028
Eam1104I	CTCTTC	6 cut(s)	96, 1575, 1629, 1974, 2292, 2801
EarI	CTCTTC	6 cut(s)	96, 1575, 1629, 1974, 2292, 2801
EciI	GGCGGA	2 cut(s)	411, 1888
Eco105I	TACGTA	1 cut(s)	429
Eco130I	CCWWGG	6 cut(s)	60, 515, 895, 1641, 2274, 2391
Eco147I	AGGCCT	1 cut(s)	325
Eco24I	GRGCYC	1 cut(s)	1025
Eco31I	GGTCTC	2 cut(s)	1021, 1722
Eco47I	GGWCC	1 cut(s)	65
Eco57I	CTGAAG	4 cut(s)	933, 981, 1335, 1815
Eco88I	CYCGRG	3 cut(s)	1018, 1608, 1967
EcoNI	CCTNNNNNAGG	1 cut(s)	1970
EcoO109I	RGGNCCY	1 cut(s)	65
EcoRI	GAATTC	1 cut(s)	1616
EcoRII	CCWGG	5 cut(s)	271, 385, 1731, 1931, 1947
EcoT14I	CCWWGG	6 cut(s)	60, 515, 895, 1641, 2274, 2391
EcoT22I	ATGCAT	1 cut(s)	763
EcoT38I	GRGCYC	1 cut(s)	1025
ErhI	CCWWGG	6 cut(s)	60, 515, 895, 1641, 2274, 2391
FaeI	CATG	8 cut(s)	64, 316, 494, 2069, 2228, 2278, 2789, 3026
FalI	AAGNNNNNCTT	2 cut(s)	577, 609
FaqI	GGGAC	2 cut(s)	183, 347
FatI	CATG	8 cut(s)	60, 312, 490, 2065, 2224, 2274, 2785, 3022
FauI	CCCGC	2 cut(s)	1174, 2010
FauNDI	CATATG	1 cut(s)	409
FblI	GTMKAC	2 cut(s)	782, 2769
Fnu4HI	GCNGC	4 cut(s)	27, 32, 1791, 2843
FokI	GGATG	7 cut(s)	211, 264, 1910, 2261, 2266, 2759, 3011
FriOI	GRGCYC	1 cut(s)	1025
Fsp4HI	GCNGC	4 cut(s)	27, 32, 1791, 2843
FspBI	CTAG	6 cut(s)	207, 570, 621, 972, 1520, 2238
FspI	TGCGCA	1 cut(s)	30
GlaI	GCGC	4 cut(s)	30, 1507, 1589, 1916
GluI	GCNGC	4 cut(s)	27, 32, 1791, 2843
GsaI	CCCAGC	2 cut(s)	439, 990
GsuI	CTGGAG	1 cut(s)	1592
HaeIII	GGCC	4 cut(s)	262, 325, 984, 2639
HapII	CCGG	3 cut(s)	1188, 1825, 2836
HgaI	GACGC	1 cut(s)	2411
HhaI	GCGC	4 cut(s)	31, 1508, 1590, 1917
Hin1II	CATG	8 cut(s)	64, 316, 494, 2069, 2228, 2278, 2789, 3026
Hin6I	GCGC	4 cut(s)	29, 1506, 1588, 1915
HinP1I	GCGC	4 cut(s)	29, 1506, 1588, 1915
HincII	GTYRAC	2 cut(s)	1136, 2426
HindII	GTYRAC	2 cut(s)	1136, 2426
HindIII	AAGCTT	2 cut(s)	1925, 2360
HpaII	CCGG	3 cut(s)	1188, 1825, 2836
HphI	GGTGA	4 cut(s)	132, 1535, 1870, 3062
Hpy188I	TCNGA	8 cut(s)	72, 646, 910, 1354, 1453, 2347, 2875, 2956
Hpy99I	CGWCG	1 cut(s)	3012
HpyCH4IV	ACGT	2 cut(s)	428, 2710
HpyF3I	CTNAG	8 cut(s)	22, 69, 257, 351, 834, 1229, 2739, 2898
HpySE526I	ACGT	2 cut(s)	428, 2710
Hsp92II	CATG	8 cut(s)	64, 316, 494, 2069, 2228, 2278, 2789, 3026
HspAI	GCGC	4 cut(s)	29, 1506, 1588, 1915
Kpn2I	TCCGGA	1 cut(s)	1187
Kzo9I	GATC	8 cut(s)	1082, 1482, 1738, 1885, 2534, 2735, 2930, 3028
LmnI	GCTCC	7 cut(s)	61, 151, 968, 1465, 1668, 1984, 3043
Lsp1109I	GCAGC	3 cut(s)	13, 43, 1802
LweI	GCATC	9 cut(s)	189, 748, 770, 1259, 1898, 2239, 2288, 2854, 3001
MaeI	CTAG	6 cut(s)	207, 570, 621, 972, 1520, 2238
MaeII	ACGT	2 cut(s)	428, 2710
MaeIII	GTNAC	9 cut(s)	288, 820, 1033, 1147, 1342, 1844, 1961, 1995, 2918
MalI	GATC	8 cut(s)	1084, 1484, 1740, 1887, 2536, 2737, 2932, 3030
MboI	GATC	8 cut(s)	1082, 1482, 1738, 1885, 2534, 2735, 2930, 3028
MfeI	CAATTG	2 cut(s)	2009, 2538
MflI	RGATCY	1 cut(s)	1738
MhlI	GDGCHC	2 cut(s)	1025, 1105
MlyI	GAGTC	6 cut(s)	839, 1165, 1282, 1339, 1646, 2903
MmeI	TCCRAC	2 cut(s)	137, 1611
Mph1103I	ATGCAT	1 cut(s)	763
MroI	TCCGGA	1 cut(s)	1187
MroXI	GAANNNNTTC	1 cut(s)	904
MseI	TTAA	7 cut(s)	372, 1511, 1542, 2124, 2304, 2352, 3066
MslI	CAYNNNNRTG	3 cut(s)	1625, 2916, 2997
MspA1I	CMGCKG	4 cut(s)	26, 953, 1274, 1793
MspI	CCGG	3 cut(s)	1188, 1825, 2836
MspR9I	CCNGG	5 cut(s)	273, 387, 1733, 1933, 1949
MunI	CAATTG	2 cut(s)	2009, 2538
Mva1269I	GAATGC	2 cut(s)	396, 1415
MvaI	CCWGG	5 cut(s)	273, 387, 1733, 1933, 1949
MvnI	CGCG	2 cut(s)	1590, 2037
NcoI	CCATGG	2 cut(s)	60, 2274
NdeI	CATATG	1 cut(s)	409
NdeII	GATC	8 cut(s)	1082, 1482, 1738, 1885, 2534, 2735, 2930, 3028
NlaIII	CATG	8 cut(s)	64, 316, 494, 2069, 2228, 2278, 2789, 3026
NlaIV	GGNNCC	6 cut(s)	66, 111, 985, 1123, 2310, 3004
NmuCI	GTSAC	5 cut(s)	288, 1342, 1844, 1961, 2918
NsbI	TGCGCA	1 cut(s)	30
NsiI	ATGCAT	1 cut(s)	763
NspI	RCATGY	3 cut(s)	316, 494, 2069
PaeR7I	CTCGAG	2 cut(s)	1018, 1967
PagI	TCATGA	1 cut(s)	2224
PceI	AGGCCT	1 cut(s)	325
PciI	ACATGT	2 cut(s)	490, 2065
PcsI	WCGNNNNNNNCGW	1 cut(s)	293
PctI	GAATGC	2 cut(s)	396, 1415
PdmI	GAANNNNTTC	1 cut(s)	904
PfeI	GAWTC	5 cut(s)	602, 1433, 1704, 1838, 2951
PflMI	CCANNNNNTGG	1 cut(s)	194
PfoI	TCCNGGA	1 cut(s)	1947
PkrI	GCNGC	4 cut(s)	28, 33, 1792, 2844
PleI	GAGTC	6 cut(s)	838, 1164, 1282, 1339, 1645, 2902
PpsI	GAGTC	6 cut(s)	838, 1164, 1282, 1339, 1645, 2902
Ppu21I	YACGTR	1 cut(s)	429
PpuMI	RGGWCCY	1 cut(s)	65
PscI	ACATGT	2 cut(s)	490, 2065
Psp5II	RGGWCCY	1 cut(s)	65
Psp6I	CCWGG	5 cut(s)	271, 385, 1731, 1931, 1947
PspFI	CCCAGC	2 cut(s)	435, 986
PspGI	CCWGG	5 cut(s)	271, 385, 1731, 1931, 1947
PspN4I	GGNNCC	6 cut(s)	66, 111, 985, 1123, 2310, 3004
PspPI	GGNCC	2 cut(s)	65, 983
PspPPI	RGGWCCY	1 cut(s)	65
PstI	CTGCAG	2 cut(s)	793, 1147
PstNI	CAGNNNCTG	3 cut(s)	605, 1151, 1328
PsuI	RGATCY	1 cut(s)	1738
PvuII	CAGCTG	4 cut(s)	26, 953, 1274, 1793
RsaI	GTAC	5 cut(s)	561, 1234, 1550, 1823, 2369
RsaNI	GTAC	5 cut(s)	560, 1233, 1549, 1822, 2368
RseI	CAYNNNNRTG	3 cut(s)	1625, 2916, 2997
SaqAI	TTAA	7 cut(s)	372, 1511, 1542, 2124, 2304, 2352, 3066
SatI	GCNGC	4 cut(s)	27, 32, 1791, 2843
Sau3AI	GATC	8 cut(s)	1082, 1482, 1738, 1885, 2534, 2735, 2930, 3028
Sau96I	GGNCC	2 cut(s)	65, 983
ScaI	AGTACT	2 cut(s)	1234, 1550
SchI	GAGTC	6 cut(s)	839, 1165, 1282, 1339, 1646, 2903
ScrFI	CCNGG	5 cut(s)	273, 387, 1733, 1933, 1949
SduI	GDGCHC	2 cut(s)	1025, 1105
SfaNI	GCATC	9 cut(s)	189, 748, 770, 1259, 1898, 2239, 2288, 2854, 3001
SfcI	CTRYAG	5 cut(s)	504, 789, 1143, 1245, 1713
Sfr274I	CTCGAG	2 cut(s)	1018, 1967
SinI	GGWCC	1 cut(s)	65
SlaI	CTCGAG	2 cut(s)	1018, 1967
SmiMI	CAYNNNNRTG	3 cut(s)	1625, 2916, 2997
SmlI	CTYRAG	4 cut(s)	1018, 1254, 1290, 1967
SmoI	CTYRAG	4 cut(s)	1018, 1254, 1290, 1967
SnaBI	TACGTA	1 cut(s)	429
SseBI	AGGCCT	1 cut(s)	325
SspI	AATATT	1 cut(s)	230
SspMI	CTAG	6 cut(s)	207, 570, 621, 972, 1520, 2238
StuI	AGGCCT	1 cut(s)	325
StyD4I	CCNGG	5 cut(s)	271, 385, 1731, 1931, 1947
StyI	CCWWGG	6 cut(s)	60, 515, 895, 1641, 2274, 2391
TaiI	ACGT	2 cut(s)	431, 2713
TaqI	TCGA	5 cut(s)	1019, 1767, 1968, 2859, 3010
TaqII	GACCGA	1 cut(s)	1195
TatI	WGTACW	3 cut(s)	559, 1232, 1548
TauI	GCSGC	1 cut(s)	2845
TfiI	GAWTC	5 cut(s)	602, 1433, 1704, 1838, 2951
Tru1I	TTAA	7 cut(s)	372, 1511, 1542, 2124, 2304, 2352, 3066
Tru9I	TTAA	7 cut(s)	372, 1511, 1542, 2124, 2304, 2352, 3066
TscAI	CASTG	5 cut(s)	111, 1167, 1233, 1874, 2965
TseFI	GTSAC	5 cut(s)	288, 1342, 1844, 1961, 2918
TseI	GCWGC	3 cut(s)	26, 31, 1790
Tsp45I	GTSAC	5 cut(s)	288, 1342, 1844, 1961, 2918
TspDTI	ATGAA	6 cut(s)	72, 1254, 1593, 2157, 2270, 2763
TspRI	CASTG	5 cut(s)	111, 1167, 1233, 1874, 2965
Van91I	CCANNNNNTGG	1 cut(s)	194
VneI	GTGCAC	1 cut(s)	1101
VpaK11BI	GGWCC	1 cut(s)	65
XagI	CCTNNNNNAGG	1 cut(s)	1970
XapI	RAATTY	5 cut(s)	916, 1222, 1616, 1829, 2565
XceI	RCATGY	3 cut(s)	316, 494, 2069
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1315
XhoI	CTCGAG	2 cut(s)	1018, 1967
XmiI	GTMKAC	2 cut(s)	782, 2769
XmnI	GAANNNNTTC	1 cut(s)	904
XspI	CTAG	6 cut(s)	207, 570, 621, 972, 1520, 2238
ZrmI	AGTACT	2 cut(s)	1234, 1550
Zsp2I	ATGCAT	1 cut(s)	763

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.