Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0008061

Physical Location & Seq

Genomic Coordinates Reverse (-)

24794396 .. 24809202

Gene Structure 14807 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g32870.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3006 bp

ATGGAGGACGCCTACGCCAGATCCGCCACCGAGGTGTTGGATTTCTTTGGCGTGGACCCGAAGAGAGGTCTCAGTGACGCTCAGGTTTCGGAACATGCTAGACTTTATGGTCGAAACGTGCTTCCGGAAGAGAAAAGGGCTTCGTTCTGGAAACTTGTCCTGAAACAGTTCGATGATTTGCTTGTTAAGATATTGATTGTCGCAGCAATCATTTCATTTGTTCTGGCTTTGATAAATGGAGATACTGGCTTGACAGCATTCTTGGAACCTTCTGTTATCCTAACGATATTGGCGGCAAATGCTGCAGTAGGAGTGATTACAGAAACTAATGCAGAAAAGGCTCTTGAGGAGCTACGCGCATACCAAGCTGATAATGCAACTGTTCTGCGCAATGGTTGCTTCTCCATACTTCCAGCAACAGAGCTTGTCCCTGGGGACATCGTTGAAGTTGCTGTGGGATGCAAAATTCCAGCCGATATGAGAATGATTGAGATGCTCAGTAATCAGTTACGCGTTGATCAGGCAATTCTTACAGGTGAGAGTTGCTCTGTGGAGAAAGATCTTGAATCCACCACTGCGACAAATGCTGTATATCAAGACAAGACAAATATTCTTTTCTCGGGTACGGTAGTTGTGGCTGGTAGGGCTAGAGCTGTTGTAGTAGGAGTTGGAAGTCAGACTGCTATGGGCGGCATACGAGACTCAATGTTGCAAACAGAAGATGAAGTAACACCATTGAAAAAGAAGCTGGACGAATTTGGTACCTTTTTGGCCAAGGTTATTGCTGGCATATGTGTACTCGTATGGATTGTAAATATAGGTCACTTTCGTGACCCTGCTCATGGTGGATTCTTGCGTGGTGCAATTCATTACTTTAAGATTGCAGTTGCACTTGCAGTTGCAGCAATCCCTGAAGGCCTTCCAGCTGTTGTTACAACTTGTTTGGCTCTTGGGACAAAACGAATGGCTCGGTTGAATGCCATTGTAAGGTCGTTGCCATCAGTTGAGACTTTAGGCTGCACTACAGTAATTTGCAGTGACAAAACTGGGACTCTGACTACCAATATGATGTCTGCCTCTAAGGTTTGTGTGCTTCACACTGTACAGCACACTCCTGTTATCTCAGAATACAGTGTCAGCGGAACTACATTTGCACCTGAAGGCACTATTTTTGACAGTACTGGCAATCAGCTTGAATGTCCAGCTCAGTCACCTTGTCTTCTTCATATAGCAATGTCTTCAGCACTTTGCAATGAATCTGTCTTGCAATATAATCCAGACAAGGGGAGCTATGAAAAAATTGGCGAGTCAACTGAAGTAGCCCTCCGTGTCCTTGCAGAGAAGATTGGTCTTCCTGGCTATGATTCTATGCCATCTTCTCTGAATTTGTTAAGCAAGCATGAACGTGCATCTTACTGTAACCACTATTGGGAGAATCATTTTAAAAAGATTTCTGTTGCAGATTTTACTCGTGATCGGAAAATGATGAGTGTCCTTTGTAGCCGAAATCAATTGCAGATTATGTTCTGCAAAGGTGCTCCAGAGAGCATTATTTCTAGATGCACAAATATTCTGTGCAATGATGATGGCTCCACTATACCATTAACTGCTAATATCCGAGCTGAGTTGGAATCAAGGTTTCACAGTTTTGCAGGAAAAGAAACCTTAAGATGTTTGGCTCTTGCTTTCAAGCGGATGCCCATGGATGTACCGACTCTCTCCCACAATGATGAGAAGGATCTTACATTCATTGGGTTGGTTGGAATGCTTGATCCACCAAGAGAGGAAGTGAAAAATGCTATGCTTTCATGCATGACTGCTGGCATACGCGTTATTGTTGTCACTGGGGATAACAAATCCACAGCTGAATCCCTCTGCCGCAAGATAGGTGCTTTTGATCACTTTGAAGATCTTTCAGGGCACTCTTTCACCGCAACTGAGTTTGAAGAATTACCAGCTTTGCAGAAAACGATAGCATTGCAACGGATGGCATTATTCACCAGGGTCGAGCCTTCTCATAAAAGGATGCTGGTTGAGGCCTTACAACGCCAGAATGAAGTGGTTGCAATGACTGGTGATGGTGTTAATGATGCACCTGCACTAAAAAAGGCAGATATTGGAATTGCCATGGGTTCAGGAACAGCAGTTGCCAAGAGTGCTTCAGATATGGTTTTGGCTGATGACAATTTTGCCACAATTGTTGCGGCTGTTGCAGAGGGAAGGGCGATTTACAATAACACGAAGCAATTCATCAGATATATGATCTCTTCAAATATTGGTGAAGTTGTGTGTATATTTGTGGCAGCTGTACTTGGAATACCTGATACACTGGCCCCTGTCCAGCTGCTCTGGGTCAACTTAGTTACTGATGGGTTACCTGCCACAGCTATTGGTTTTAATAAGCAAGATTCTGATGTGATGAAGGCTAAACCTCGAAAGGTTAACGAAGCAGTTGTTACTGGATGGTTGTTTTTTCGTTATTTGGTGATTGGAGCTTATGTTGGGCTTGCCACAGTTGCGGGATTTATTTGGTGGTTTCTTTACTCTGACACTGGTCCTAAACTGCCATATACTGAACTGATTAATTTTGACACATGCGGAACAAGGGACACAACTTATCCATGCAGTATATTTAGTGATCGCCATCCATCAACTGTCTCCATGACCGTTCTTGTGGTTGTTGAAATGTTCAACGCATTGAATAATCTCAGTGAAAACCAATCGCTTCTTGTCATCCCTCCCTGGAGTAACTTATGGCTTGTTGGTTCAATCATTATAACAATGATCCTTCACGTATTAATATTATATGTGCCTCCCCTCTCGGTTCTTTTCTCTGTAACACCCTTATCTTGGGCTGAGTGGACTGTTGTGCTATACCTTTCATTTCCAGTTATAATCATCGATGAAGTGCTAAAGTTCTTCTCAAGAAGTACCACGGGTCTAAGATTAAATTTCCTGTTAAGGAGGCATGATCTACTGCCAAGAAAGGAATTGCGTGATAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0003824 GO:0005215 GO:0005384 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006828 GO:0007275 GO:0008150 GO:0008324 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015410 GO:0015662 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022622 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055071 GO:0055076 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071421 GO:0072511 GO:0090662 GO:0097708 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0099131 GO:0099132 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04020 ko04022 ko04972 ko05010 map04020 map04022 map04972 map05010

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1002

Amino Acids

109.4

Weight (kDa)

5.86

Isoelectric Point (pI)

37.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	5 - 72	5e-20	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	122 - 235	3.7e-24	P-type ATPase actuator domain
Cation_ATPase	PF13246	409 - 596	2.5e-41	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	579 - 705	5.8e-15	haloacid dehalogenase-like hydrolase
Hydrolase_3	PF08282	677 - 732	2.3e-06	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	776 - 974	1.4e-46	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10130
fragaria_vesca	FvH4_2g32870 FvH4_2g32870 FvH4_2g32870
malus_domestica	MD08G1080200.v1.1 MD15G1067300.v1.1
prunus_persica	Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1
pyrus_communis	pycom08g06620 pycom15g06380
rosa_chinensis	RchiOBHm_Chr6g0309841
rosa_laevigata	RLG00000010530
rosa_multiflora	Rmu_ssc0000373.1_g000002
rosa_roxburghii	Rroxscaffold_7G00159130
rosa_rugosa	Rorug06G0375200 Rorug06G0375300
rosa_samantha	Rh6AG488700 Rh6BG498200 Rh6CG503000 Rh6DG489200
rosa_wichuraiana	Rw6G042560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2777, 2894
AarI	CACCTGC	1 cut(s)	2104
AasI	GACNNNNNNGTC	1 cut(s)	108
Acc16I	TGCGCA	1 cut(s)	389
Acc36I	ACCTGC	2 cut(s)	2104, 2386
Acc65I	GGTACC	1 cut(s)	761
AccB1I	GGYRCC	1 cut(s)	761
AccII	CGCG	3 cut(s)	357, 513, 1830
AccIII	TCCGGA	1 cut(s)	124
AclWI	GGATC	4 cut(s)	15, 1746, 1766, 2779
AcoI	YGGCCR	1 cut(s)	771
AcsI	RAATTY	4 cut(s)	465, 755, 1384, 2950
AcuI	CTGAAG	5 cut(s)	933, 1179, 1224, 1335, 2145
AcyI	GRCGYC	1 cut(s)	9
AfaI	GTAC	8 cut(s)	625, 763, 798, 1104, 1180, 1710, 2310, 2932
AfiI	CCNNNNNNNGG	6 cut(s)	65, 842, 987, 1283, 1429, 2850
AflII	CTTAAG	1 cut(s)	1666
AflIII	ACRYGT	2 cut(s)	511, 1828
AjnI	CCWGG	4 cut(s)	430, 1354, 2000, 2741
AleI	CACNNNNGTG	2 cut(s)	32, 828
Alw21I	GWGCWC	1 cut(s)	1540
Alw26I	GTCTC	4 cut(s)	74, 693, 1001, 2662
AlwI	GGATC	4 cut(s)	15, 1746, 1766, 2779
Ama87I	CYCGRG	1 cut(s)	619
Aor13HI	TCCGGA	1 cut(s)	124
AoxI	GGCC	4 cut(s)	771, 916, 2037, 2331
ApeKI	GCWGC	6 cut(s)	203, 302, 902, 1017, 2303, 2344
ApoI	RAATTY	4 cut(s)	465, 755, 1384, 2950
AseI	ATTAAT	2 cut(s)	2583, 2798
Asp700I	GAANNNNTTC	3 cut(s)	167, 918, 2246
Asp718I	GGTACC	1 cut(s)	761
AspLEI	GCGC	2 cut(s)	359, 390
AspS9I	GGNCC	3 cut(s)	55, 2332, 2555
AsuHPI	GGTGA	7 cut(s)	548, 1203, 1921, 1990, 2087, 2291, 2497
AvaI	CYCGRG	1 cut(s)	619
AvaII	GGWCC	2 cut(s)	55, 2555
BaeGI	GKGCMC	1 cut(s)	1923
BaeI	ACNNNNGTAYC	2 cut(s)	615, 648
BalI	TGGCCA	1 cut(s)	773
BanI	GGYRCC	1 cut(s)	761
BauI	CACGAG	1 cut(s)	1470
BbsI	GAAGAC	3 cut(s)	1211, 1230, 1343
Bbv12I	GWGCWC	1 cut(s)	1540
BbvI	GCAGC	6 cut(s)	215, 289, 914, 1004, 2315, 2331
BccI	CCATC	9 cut(s)	1006, 1381, 1580, 1981, 2072, 2363, 2457, 2652, 2656
BcgI	CGANNNNNNTGC	2 cut(s)	1960, 1994
BciT130I	CCWGG	4 cut(s)	432, 1356, 2002, 2743
BclI	TGATCA	2 cut(s)	517, 1897
BcoDI	GTCTC	4 cut(s)	74, 693, 1001, 2662
BfaI	CTAG	3 cut(s)	99, 648, 1557
BfmI	CTRYAG	2 cut(s)	303, 1023
BfrI	CTTAAG	1 cut(s)	1666
BfuAI	ACCTGC	2 cut(s)	2104, 2386
BglII	AGATCT	2 cut(s)	559, 1909
BmcAI	AGTACT	1 cut(s)	1180
Bme1390I	CCNGG	4 cut(s)	432, 1356, 2002, 2743
Bme18I	GGWCC	2 cut(s)	55, 2555
BmeT110I	CYCGRG	1 cut(s)	619
BmgT120I	GGNCC	3 cut(s)	55, 2332, 2555
BmiI	GGNNCC	5 cut(s)	57, 267, 763, 1591, 2334
BmrFI	CCNGG	4 cut(s)	432, 1356, 2002, 2743
BmrI	ACTGGG	2 cut(s)	1056, 1854
BmsI	GCATC	7 cut(s)	449, 483, 1418, 1550, 1686, 2016, 2080
BmuI	ACTGGG	2 cut(s)	1056, 1854
BoxI	GACNNNNGTC	1 cut(s)	2553
BpiI	GAAGAC	3 cut(s)	1211, 1230, 1343
BplI	GAGNNNNNCTC	2 cut(s)	530, 562
BpmI	CTGGAG	2 cut(s)	1524, 2764
Bpu10I	CCTNAGC	1 cut(s)	81
BpuEI	CTTGAG	2 cut(s)	365, 2908
Bsa29I	ATCGAT	1 cut(s)	2901
BsaAI	YACGTR	1 cut(s)	2794
BsaBI	GATNNNNATC	1 cut(s)	2643
BsaHI	GRCGYC	1 cut(s)	9
BsaI	GGTCTC	1 cut(s)	74
BsaJI	CCNNGG	9 cut(s)	30, 430, 431, 774, 1701, 2001, 2127, 2741, 2934
BsaWI	WCCGGW	1 cut(s)	124
BsaXI	ACNNNNNCTCC	2 cut(s)	2484, 2514
Bsc4I	CCNNNNNNNGG	6 cut(s)	65, 842, 987, 1283, 1429, 2850
Bse1I	ACTGG	9 cut(s)	250, 1051, 1186, 1849, 2077, 2334, 2464, 2557, 2888
Bse3DI	GCAATG	6 cut(s)	397, 1239, 1258, 1585, 1976, 2073
Bse8I	GATNNNNATC	1 cut(s)	2643
BseAI	TCCGGA	1 cut(s)	124
BseBI	CCWGG	4 cut(s)	432, 1356, 2002, 2743
BseCI	ATCGAT	1 cut(s)	2901
BseDI	CCNNGG	9 cut(s)	30, 430, 431, 774, 1701, 2001, 2127, 2741, 2934
BseGI	GGATG	8 cut(s)	464, 1701, 1711, 1992, 2031, 2468, 2644, 2733
BseJI	GATNNNNATC	1 cut(s)	2643
BseLI	CCNNNNNNNGG	6 cut(s)	65, 842, 987, 1283, 1429, 2850
BseMI	GCAATG	6 cut(s)	397, 1239, 1258, 1585, 1976, 2073
BseMII	CTCAG	9 cut(s)	85, 95, 511, 1137, 1220, 1614, 1929, 2722, 2847
BseNI	ACTGG	9 cut(s)	250, 1051, 1186, 1849, 2077, 2334, 2464, 2557, 2888
BseRI	GAGGAG	1 cut(s)	362
BseSI	GKGCMC	1 cut(s)	1923
BseXI	GCAGC	6 cut(s)	215, 289, 914, 1004, 2315, 2331
BsgI	GTGCAG	2 cut(s)	1003, 2082
Bsh1236I	CGCG	3 cut(s)	357, 513, 1830
BshFI	GGCC	4 cut(s)	773, 918, 2039, 2333
BshNI	GGYRCC	1 cut(s)	761
BshVI	ATCGAT	1 cut(s)	2901
BsiHKAI	GWGCWC	1 cut(s)	1540
BsiHKCI	CYCGRG	1 cut(s)	619
BsiSI	CCGG	1 cut(s)	125
BslFI	GGGAC	5 cut(s)	413, 449, 967, 1063, 2621
BslI	CCNNNNNNNGG	6 cut(s)	65, 842, 987, 1283, 1429, 2850
BsmAI	GTCTC	4 cut(s)	74, 693, 1001, 2662
BsmFI	GGGAC	5 cut(s)	413, 449, 967, 1063, 2621
BsmI	GAATGC	3 cut(s)	257, 982, 1770
BsnI	GGCC	4 cut(s)	773, 918, 2039, 2333
Bso31I	GGTCTC	1 cut(s)	74
BsoBI	CYCGRG	1 cut(s)	619
Bsp1286I	GDGCHC	2 cut(s)	1540, 1923
Bsp13I	TCCGGA	1 cut(s)	124
Bsp1407I	TGTACA	1 cut(s)	1102
Bsp19I	CCATGG	2 cut(s)	1701, 2127
BspANI	GGCC	4 cut(s)	773, 918, 2039, 2333
BspCNI	CTCAG	9 cut(s)	84, 94, 510, 1136, 1219, 1615, 1930, 2721, 2848
BspDI	ATCGAT	1 cut(s)	2901
BspEI	TCCGGA	1 cut(s)	124
BspFNI	CGCG	3 cut(s)	357, 513, 1830
BspLI	GGNNCC	5 cut(s)	57, 267, 763, 1591, 2334
BspMAI	CTGCAG	1 cut(s)	307
BspMI	ACCTGC	2 cut(s)	2104, 2386
BspPI	GGATC	4 cut(s)	15, 1746, 1766, 2779
BspT107I	GGYRCC	1 cut(s)	761
BspTI	CTTAAG	1 cut(s)	1666
BspTNI	GGTCTC	1 cut(s)	74
BsrDI	GCAATG	6 cut(s)	397, 1239, 1258, 1585, 1976, 2073
BsrGI	TGTACA	1 cut(s)	1102
BsrI	ACTGG	9 cut(s)	250, 1051, 1186, 1849, 2077, 2334, 2464, 2557, 2888
BssECI	CCNNGG	9 cut(s)	30, 430, 431, 774, 1701, 2001, 2127, 2741, 2934
BssNI	GRCGYC	1 cut(s)	9
BssSI	CACGAG	1 cut(s)	1470
BssT1I	CCWWGG	3 cut(s)	774, 1701, 2127
Bst2BI	CACGAG	1 cut(s)	1470
Bst2UI	CCWGG	4 cut(s)	432, 1356, 2002, 2743
Bst6I	CTCTTC	3 cut(s)	56, 123, 2272
BstACI	GRCGYC	1 cut(s)	9
BstAFI	CTTAAG	1 cut(s)	1666
BstAPI	GCANNNNNTGC	2 cut(s)	302, 396
BstAUI	TGTACA	1 cut(s)	1102
BstBAI	YACGTR	1 cut(s)	2794
BstC8I	GCNNGC	4 cut(s)	787, 1397, 1822, 2508
BstDSI	CCRYGG	3 cut(s)	1701, 2127, 2934
BstEII	GGTNACC	1 cut(s)	2373
BstF5I	GGATG	8 cut(s)	464, 1701, 1711, 1992, 2031, 2468, 2644, 2733
BstFNI	CGCG	3 cut(s)	357, 513, 1830
BstHHI	GCGC	2 cut(s)	359, 390
BstMAI	GTCTC	4 cut(s)	74, 693, 1001, 2662
BstNI	CCWGG	4 cut(s)	432, 1356, 2002, 2743
BstNSI	RCATGY	2 cut(s)	98, 2598
BstPAI	GACNNNNGTC	1 cut(s)	2553
BstPI	GGTNACC	1 cut(s)	2373
BstSCI	CCNGG	4 cut(s)	430, 1354, 2000, 2741
BstSFI	CTRYAG	2 cut(s)	303, 1023
BstSLI	GKGCMC	1 cut(s)	1923
BstUI	CGCG	3 cut(s)	357, 513, 1830
BstV1I	GCAGC	6 cut(s)	215, 289, 914, 1004, 2315, 2331
BstV2I	GAAGAC	3 cut(s)	1211, 1230, 1343
BstX2I	RGATCY	4 cut(s)	20, 559, 1738, 1909
BstYI	RGATCY	4 cut(s)	20, 559, 1738, 1909
Bsu15I	ATCGAT	1 cut(s)	2901
BsuRI	GGCC	4 cut(s)	773, 918, 2039, 2333
BsuTUI	ATCGAT	1 cut(s)	2901
BtgI	CCRYGG	3 cut(s)	1701, 2127, 2934
BtsCI	GGATG	8 cut(s)	464, 1701, 1711, 1992, 2031, 2468, 2644, 2733
BtsI	GCAGTG	2 cut(s)	573, 1042
BtsIMutI	CAGTG	9 cut(s)	79, 573, 1042, 1098, 1138, 1842, 2327, 2550, 2716
BveI	ACCTGC	2 cut(s)	2104, 2386
Cac8I	GCNNGC	4 cut(s)	787, 1397, 1822, 2508
CfoI	GCGC	2 cut(s)	359, 390
Cfr13I	GGNCC	3 cut(s)	55, 2332, 2555
ClaI	ATCGAT	1 cut(s)	2901
CseI	GACGC	2 cut(s)	17, 86
Csp6I	GTAC	8 cut(s)	624, 762, 797, 1103, 1179, 1709, 2309, 2931
CspCI	CAANNNNNGTGG	4 cut(s)	2182, 2217, 2371, 2406
CviQI	GTAC	8 cut(s)	624, 762, 797, 1103, 1179, 1709, 2309, 2931
DraI	TTTAAA	1 cut(s)	1444
DrdI	GACNNNNNNGTC	1 cut(s)	108
DseDI	GACNNNNNNGTC	1 cut(s)	108
EaeI	YGGCCR	1 cut(s)	771
Eam1104I	CTCTTC	3 cut(s)	56, 123, 2272
EarI	CTCTTC	3 cut(s)	56, 123, 2272
EciI	GGCGGA	1 cut(s)	13
Eco130I	CCWWGG	3 cut(s)	774, 1701, 2127
Eco147I	AGGCCT	2 cut(s)	918, 2039
Eco31I	GGTCTC	1 cut(s)	74
Eco47I	GGWCC	2 cut(s)	55, 2555
Eco57I	CTGAAG	5 cut(s)	933, 1179, 1224, 1335, 2145
Eco88I	CYCGRG	1 cut(s)	619
Eco91I	GGTNACC	1 cut(s)	2373
EcoO65I	GGTNACC	1 cut(s)	2373
EcoRII	CCWGG	4 cut(s)	430, 1354, 2000, 2741
EcoT14I	CCWWGG	3 cut(s)	774, 1701, 2127
EcoT22I	ATGCAT	1 cut(s)	1814
ErhI	CCWWGG	3 cut(s)	774, 1701, 2127
FaqI	GGGAC	5 cut(s)	413, 449, 967, 1063, 2621
FauI	CCCGC	1 cut(s)	2512
FauNDI	CATATG	1 cut(s)	791
FbaI	TGATCA	2 cut(s)	517, 1897
FokI	GGATG	8 cut(s)	471, 1708, 1718, 1999, 2038, 2475, 2631, 2720
FspBI	CTAG	3 cut(s)	99, 648, 1557
FspI	TGCGCA	1 cut(s)	389
GlaI	GCGC	2 cut(s)	358, 389
GsuI	CTGGAG	2 cut(s)	1524, 2764
HaeIII	GGCC	4 cut(s)	773, 918, 2039, 2333
HapII	CCGG	1 cut(s)	125
HgaI	GACGC	2 cut(s)	17, 86
HhaI	GCGC	2 cut(s)	359, 390
Hin1I	GRCGYC	1 cut(s)	9
Hin6I	GCGC	2 cut(s)	357, 388
HinP1I	GCGC	2 cut(s)	357, 388
HincII	GTYRAC	3 cut(s)	1311, 2356, 2443
HindII	GTYRAC	3 cut(s)	1311, 2356, 2443
HpaI	GTTAAC	1 cut(s)	2443
HpaII	CCGG	1 cut(s)	125
HphI	GGTGA	7 cut(s)	548, 1203, 1921, 1990, 2087, 2291, 2497
Hpy166II	GTNNAC	6 cut(s)	55, 797, 1311, 2356, 2443, 2862
Hpy8I	GTNNAC	6 cut(s)	55, 797, 1311, 2356, 2443, 2862
HpyAV	CCTTC	9 cut(s)	279, 908, 929, 1154, 1729, 2022, 2214, 2416, 2798
HpyCH4IV	ACGT	3 cut(s)	117, 1405, 2793
HpySE526I	ACGT	3 cut(s)	117, 1405, 2793
Hsp92I	GRCGYC	1 cut(s)	9
HspAI	GCGC	2 cut(s)	357, 388
Kpn2I	TCCGGA	1 cut(s)	124
KpnI	GGTACC	1 cut(s)	765
Ksp22I	TGATCA	2 cut(s)	517, 1897
KspAI	GTTAAC	1 cut(s)	2443
LmnI	GCTCC	5 cut(s)	349, 1287, 1543, 1595, 2492
Lsp1109I	GCAGC	6 cut(s)	215, 289, 914, 1004, 2315, 2331
LweI	GCATC	7 cut(s)	449, 483, 1418, 1550, 1686, 2016, 2080
MaeI	CTAG	3 cut(s)	99, 648, 1557
MaeII	ACGT	3 cut(s)	117, 1405, 2793
MfeI	CAATTG	2 cut(s)	1511, 2196
MflI	RGATCY	4 cut(s)	20, 559, 1738, 1909
MhlI	GDGCHC	2 cut(s)	1540, 1923
MlsI	TGGCCA	1 cut(s)	773
MluI	ACGCGT	2 cut(s)	511, 1828
MluNI	TGGCCA	1 cut(s)	773
MlyI	GAGTC	4 cut(s)	695, 1045, 1316, 1708
MmeI	TCCRAC	4 cut(s)	18, 649, 1608, 1741
Mox20I	TGGCCA	1 cut(s)	773
Mph1103I	ATGCAT	1 cut(s)	1814
MroI	TCCGGA	1 cut(s)	124
MroXI	GAANNNNTTC	3 cut(s)	167, 918, 2246
MscI	TGGCCA	1 cut(s)	773
MslI	CAYNNNNRTG	4 cut(s)	32, 828, 1404, 1728
Msp20I	TGGCCA	1 cut(s)	773
MspA1I	CMGCKG	5 cut(s)	926, 1140, 1865, 2306, 2344
MspCI	CTTAAG	1 cut(s)	1666
MspI	CCGG	1 cut(s)	125
MspR9I	CCNGG	4 cut(s)	432, 1356, 2002, 2743
MunI	CAATTG	2 cut(s)	1511, 2196
Mva1269I	GAATGC	3 cut(s)	257, 982, 1770
MvaI	CCWGG	4 cut(s)	432, 1356, 2002, 2743
MvnI	CGCG	3 cut(s)	357, 513, 1830
NcoI	CCATGG	2 cut(s)	1701, 2127
NdeI	CATATG	1 cut(s)	791
NlaIV	GGNNCC	5 cut(s)	57, 267, 763, 1591, 2334
NmuCI	GTSAC	6 cut(s)	74, 821, 830, 1037, 1209, 1840
NsbI	TGCGCA	1 cut(s)	389
NsiI	ATGCAT	1 cut(s)	1814
NspI	RCATGY	2 cut(s)	98, 2598
OliI	CACNNNNGTG	2 cut(s)	32, 828
PaqCI	CACCTGC	1 cut(s)	2104
PasI	CCCWGGG	1 cut(s)	431
PceI	AGGCCT	2 cut(s)	918, 2039
PctI	GAATGC	3 cut(s)	257, 982, 1770
PdmI	GAANNNNTTC	3 cut(s)	167, 918, 2246
PfeI	GAWTC	8 cut(s)	566, 849, 1256, 1364, 1435, 1631, 1868, 2408
PleI	GAGTC	4 cut(s)	695, 1045, 1315, 1708
PpsI	GAGTC	4 cut(s)	695, 1045, 1315, 1708
Ppu21I	YACGTR	1 cut(s)	2794
PshAI	GACNNNNGTC	1 cut(s)	2553
PshBI	ATTAAT	2 cut(s)	2583, 2798
PsiI	TTATAA	2 cut(s)	2777, 2894
Psp6I	CCWGG	4 cut(s)	430, 1354, 2000, 2741
PspEI	GGTNACC	1 cut(s)	2373
PspGI	CCWGG	4 cut(s)	430, 1354, 2000, 2741
PspN4I	GGNNCC	5 cut(s)	57, 267, 763, 1591, 2334
PspPI	GGNCC	3 cut(s)	55, 2332, 2555
PstI	CTGCAG	1 cut(s)	307
PsuI	RGATCY	4 cut(s)	20, 559, 1738, 1909
PvuII	CAGCTG	4 cut(s)	926, 1865, 2306, 2344
RsaI	GTAC	8 cut(s)	625, 763, 798, 1104, 1180, 1710, 2310, 2932
RsaNI	GTAC	8 cut(s)	624, 762, 797, 1103, 1179, 1709, 2309, 2931
RseI	CAYNNNNRTG	4 cut(s)	32, 828, 1404, 1728
Sau96I	GGNCC	3 cut(s)	55, 2332, 2555
ScaI	AGTACT	1 cut(s)	1180
SchI	GAGTC	4 cut(s)	695, 1045, 1316, 1708
ScrFI	CCNGG	4 cut(s)	432, 1356, 2002, 2743
SduI	GDGCHC	2 cut(s)	1540, 1923
SfaNI	GCATC	7 cut(s)	449, 483, 1418, 1550, 1686, 2016, 2080
SfcI	CTRYAG	2 cut(s)	303, 1023
SinI	GGWCC	2 cut(s)	55, 2555
SmiMI	CAYNNNNRTG	4 cut(s)	32, 828, 1404, 1728
SmlI	CTYRAG	3 cut(s)	344, 1666, 2923
SmoI	CTYRAG	3 cut(s)	344, 1666, 2923
SseBI	AGGCCT	2 cut(s)	918, 2039
SspI	AATATT	4 cut(s)	610, 1570, 2275, 2802
SspMI	CTAG	3 cut(s)	99, 648, 1557
StuI	AGGCCT	2 cut(s)	918, 2039
StyD4I	CCNGG	4 cut(s)	430, 1354, 2000, 2741
StyI	CCWWGG	3 cut(s)	774, 1701, 2127
TaiI	ACGT	3 cut(s)	120, 1408, 2796
TaqI	TCGA	5 cut(s)	112, 171, 2007, 2434, 2901
TatI	WGTACW	4 cut(s)	796, 1102, 1178, 2308
TauI	GCSGC	4 cut(s)	296, 693, 1881, 2207
TfiI	GAWTC	8 cut(s)	566, 849, 1256, 1364, 1435, 1631, 1868, 2408
TscAI	CASTG	9 cut(s)	79, 580, 1042, 1105, 1138, 1849, 2334, 2557, 2716
TseFI	GTSAC	6 cut(s)	74, 821, 830, 1037, 1209, 1840
TseI	GCWGC	6 cut(s)	203, 302, 902, 1017, 2303, 2344
Tsp45I	GTSAC	6 cut(s)	74, 821, 830, 1037, 1209, 1840
TspGWI	ACGGA	2 cut(s)	1316, 1999
TspRI	CASTG	9 cut(s)	79, 580, 1042, 1105, 1138, 1849, 2334, 2557, 2716
Vha464I	CTTAAG	1 cut(s)	1666
VpaK11BI	GGWCC	2 cut(s)	55, 2555
VspI	ATTAAT	2 cut(s)	2583, 2798
XapI	RAATTY	4 cut(s)	465, 755, 1384, 2950
XbaI	TCTAGA	1 cut(s)	1556
XceI	RCATGY	2 cut(s)	98, 2598
XcmI	CCANNNNNNNNNTGG	1 cut(s)	34
XmnI	GAANNNNTTC	3 cut(s)	167, 918, 2246
XspI	CTAG	3 cut(s)	99, 648, 1557
ZrmI	AGTACT	1 cut(s)	1180
Zsp2I	ATGCAT	1 cut(s)	1814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.