Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0008061

Physical Location & Seq

Genomic Coordinates Forward (+)

64977261 .. 64992229

Gene Structure 14969 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG489200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2613 bp

ATGGAGGACGCCTACGCCAGATCCGCCACCGAGGTGTTGGATTTCTTTGGCGTGGACCCGAAACGAGGTCTCAGTGACACTCAGGTTTCGCAACATGCTAGACTTTATGGACAAAACGTGCTTCCGGAAGAGAAAAGGGCTTCGTTCTGGAAACTCGTCCTGAAACAGTTTGATGATTTGCTTGTTAAGATATTGATTGTTGCAGCAATCGTTTCTTTTGTTCTGGCTCTAATTAATGGAGATACGGGCTTAACAGCATTTTTGGAGCCTTCTGTTATCCTCACGATATTGGCTGCAAATGCTGCAGTAGGAGTGATTACAGAAACTAATGCAGAAAAGGCTCTTGAGGAGCTACGCGCATACCAAGCTGATAACGCAACCGTTCTGCGCAATGGTTGCTTCTCCATACTTCCAGCAACAGAGCTCGTCCCTGGGGATATCGTTGAAGTGGCTGTGGGATGCAAAATTCCAGCCGATATGAGAATGATTGAGATGCTCAGTAATCAATTACGTGTTGATCAGGCAATCCTTACAGGTGAGAGTTGCTCTGTGGAGAAAGATCTTGAATCCACCACTGCAACAAATGCTGTATATCAAGACAAGACAAATATTCTTTTCTCGGGTACGGTAGTCGTGGCTGGTAGGGCTAGAGCTGTTGTAGTAAGAGTTGGAACTCAGACTGCTATGGGCGGCATACGAGACTCAATGTTGCAAACGGAAGATGAAGTGACACCATTGAAAAAGAAGCTGGATGAATTTGGTACCTTTTTGGCCAAGGTTATTGCTGGCATATGTGTACTCGTATGGATTGTAAATATAGGTCACTTTCGTGACCCTGCTCATGGTGGATTCTTGCGTGGTGCAATTCATTACTTTAAGATTGCAGTTGCACTTGCAGTTGCAGCAATCCCTGAAGGCCTTCCTGCAGTTGTTACAACTTGTTTGGCTCTTGGGACAAAACGAATGGCTAGGTTGAATGCCATTGTAAGGTCATTGCCATCAGTTGAGACCCTAGGCTGCACTACAGTAATTTGCAGTGACAAAACTGGGACTCTGACTACCAATATGATGTCTGCCTCTAAGGTTTGTGTGCTTCACACTGTACAGCACATTCCTGTTATCTCAGAATACAGTGTCAGCGGAACTACATTTGCACCTGAAGGCACTATTTTTGACAGTACTGGCAATCAGCTTGAATTTCCAGCTCAATTGCCTTGTCTTCTTCACATAGCAATGGCTTCAGCGCTTTGCAATGAATCTGTCTTGCAATATAATCCAGACAAGGGGAGCTATGAAAAAATTGGCGAGTCAACTGAAGTAGCCCTCCGTGTCCTTGCAGAGAAGATTGGTCTTCCCGGCTATGATTCTATGCCATCTTCTCTGAATTTGCTAAGCAAGCATGAACGCGCATCTTACTGTAACCATTATTGGGAGAATCAATTTAAAAAGATTTCTGTTGCAGATTTTACTCGTGATCGTAAAATGATGAGTGTCCTTTGTAGCCGAAGTCAGTTGCAGATTATGTTCTGCAAAGGTGCTCCAGAGAGCATTATTTCTAGATGCACCAATATTCTGTGCAATGATGATGGCTCCACTATACCATTAACTGCTAATATCCGAGCTGAGTTGGAATCAAGGTTCCACAGTTTTGCAGGAAAAGAAACCTTAAGATGCTTGGCTCTTGCTTTCAAGCGGATGCCGATGGATGTACCGACTCTCTCCCACAATGATGAGAAGGATCTTACATTCATTGGGTTGGTCGGAATGCTTGATCCACCAAGAGAGGAAGTGAAAAATGCTATGCTTTCATGCATGACTGCTGGCATACGCGTTATAGTTGTCACCGGGGATAACAAATCCACAGCTGAATCCCTTTGCCGCAAGATAGGTGCTTTTGATCACTTTGTAGATCTTGCTGGGCACTCTTACACCGCAACTGAGTTTGAAGAGTTACCAGCTTTGCAGAAAACGATAGCATTGCAACGGATGGCATTATTCACCAGGGTCGAGCCTTCTCATAAAAGGATGCTGGTTGAGGCCTTACAACACCAGAATGAAGTGGTTGCAATGACTGGTGATGGTGTTAATGATGCACCTGCACTAAAGAAGGCAGACATTGGAATTGCCATGGGTTCAGGAACAGCTGTTGCCAAGAGTGCTTCAGATATGGTTTTGGCTGATGACAATTTTGCCACTATTGTTGCGGCTGTTGCAGAGGGAAGGGCAATTTACAATAACACAAAACAATTCATCAGATATATGATCTCTTCAAATATTGGTGAAGTTGTTTGTATATTTGTGGCAGCTGTACTTGGAATACCTGATACACTGGCCCCTGTCCAGCTGCTCTGGGTCAACTTAGTTACTGATGGGTTGCCTGCCACAGCAATTGGTTTTAATAAGCAAGATTCTGATGTGATGAAGGCTAAACCTCGTAAGGTTAATGAAGCAGTTGTTACTGGATGGTTGTTTTTTCGTTATTTGGTGATTGGAGCTTATGTTGGGCTTGCCACAGTTGCTGGATTTATTTGGTGGTTTCTTTACTCTGATACCGGTCCTAAACTGCCATATATGGAACTGGTAAGATTCTATAGGCTGTGTTCAGTACTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0003824 GO:0005215 GO:0005384 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006828 GO:0007275 GO:0008150 GO:0008324 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015410 GO:0015662 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022622 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055071 GO:0055076 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071421 GO:0072511 GO:0090662 GO:0097708 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0099131 GO:0099132 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04020 ko04022 ko04972 ko05010 map04020 map04022 map04972 map05010

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

870

Amino Acids

94.58

Weight (kDa)

5.93

Isoelectric Point (pI)

36.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	5 - 72	1.5e-19	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	122 - 235	1.5e-24	P-type ATPase actuator domain
Cation_ATPase	PF13246	409 - 596	1.4e-41	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	578 - 705	1.7e-14	haloacid dehalogenase-like hydrolase
Hydrolase_3	PF08282	677 - 732	2.6e-06	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	776 - 849	8.5e-21	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10130
fragaria_vesca	FvH4_2g32870 FvH4_2g32870 FvH4_2g32870
malus_domestica	MD08G1080200.v1.1 MD15G1067300.v1.1
prunus_persica	Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1
pyrus_communis	pycom08g06620 pycom15g06380
rosa_chinensis	RchiOBHm_Chr6g0309841
rosa_laevigata	RLG00000010530
rosa_multiflora	Rmu_ssc0000373.1_g000002
rosa_roxburghii	Rroxscaffold_7G00159130
rosa_rugosa	Rorug06G0375200 Rorug06G0375300
rosa_samantha	Rh6AG488700 Rh6BG498200 Rh6CG503000 Rh6DG489200
rosa_wichuraiana	Rw6G042560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2104
Acc16I	TGCGCA	1 cut(s)	389
Acc36I	ACCTGC	1 cut(s)	2104
Acc65I	GGTACC	1 cut(s)	761
AccB1I	GGYRCC	1 cut(s)	761
AccII	CGCG	3 cut(s)	357, 1407, 1830
AccIII	TCCGGA	1 cut(s)	124
AciI	CCGC	7 cut(s)	24, 690, 1140, 1693, 1879, 1932, 2204
AclWI	GGATC	3 cut(s)	15, 1746, 1766
AcoI	YGGCCR	1 cut(s)	771
AcsI	RAATTY	4 cut(s)	465, 755, 1196, 1384
AcuI	CTGAAG	5 cut(s)	933, 1179, 1224, 1335, 2145
AcyI	GRCGYC	1 cut(s)	9
AfaI	GTAC	8 cut(s)	625, 763, 798, 1104, 1180, 1710, 2310, 2607
AfeI	AGCGCT	1 cut(s)	1245
AfiI	CCNNNNNNNGG	5 cut(s)	65, 842, 987, 1283, 1429
AflII	CTTAAG	1 cut(s)	1666
AflIII	ACRYGT	2 cut(s)	511, 1828
AgeI	ACCGGT	1 cut(s)	2552
AgsI	TTSAA	8 cut(s)	446, 566, 739, 976, 1196, 1690, 1946, 2271
AjnI	CCWGG	2 cut(s)	430, 2000
AleI	CACNNNNGTG	2 cut(s)	32, 828
AloI	GAACNNNNNNTCC	2 cut(s)	1622, 1654
Alw21I	GWGCWC	2 cut(s)	426, 1540
Alw26I	GTCTC	3 cut(s)	74, 693, 1001
AlwI	GGATC	3 cut(s)	15, 1746, 1766
AlwNI	CAGNNNCTG	1 cut(s)	2519
Ama87I	CYCGRG	1 cut(s)	619
Aor13HI	TCCGGA	1 cut(s)	124
Aor51HI	AGCGCT	1 cut(s)	1245
AoxI	GGCC	4 cut(s)	771, 916, 2037, 2331
ApeKI	GCWGC	7 cut(s)	203, 293, 302, 902, 1017, 2303, 2344
ApoI	RAATTY	4 cut(s)	465, 755, 1196, 1384
AseI	ATTAAT	1 cut(s)	234
AsiGI	ACCGGT	1 cut(s)	2552
Asp700I	GAANNNNTTC	1 cut(s)	918
Asp718I	GGTACC	1 cut(s)	761
AspA2I	CCTAGG	1 cut(s)	1012
AspLEI	GCGC	4 cut(s)	359, 390, 1246, 1409
AspS9I	GGNCC	3 cut(s)	55, 2332, 2555
AsuC2I	CCSGG	2 cut(s)	1356, 1846
AsuHPI	GGTGA	6 cut(s)	548, 1834, 1990, 2087, 2291, 2497
AvaI	CYCGRG	1 cut(s)	619
AvaII	GGWCC	2 cut(s)	55, 2555
AvrII	CCTAGG	1 cut(s)	1012
BaeGI	GKGCMC	1 cut(s)	1923
BaeI	ACNNNNGTAYC	2 cut(s)	615, 648
BalI	TGGCCA	1 cut(s)	773
BanI	GGYRCC	1 cut(s)	761
BanII	GRGCYC	1 cut(s)	426
BauI	CACGAG	1 cut(s)	1470
BbsI	GAAGAC	2 cut(s)	1211, 1343
Bbv12I	GWGCWC	2 cut(s)	426, 1540
BbvI	GCAGC	7 cut(s)	215, 280, 289, 914, 1004, 2315, 2331
BccI	CCATC	8 cut(s)	1006, 1381, 1580, 1696, 1981, 2072, 2363, 2457
BcgI	CGANNNNNNTGC	2 cut(s)	1960, 1994
BciT130I	CCWGG	2 cut(s)	432, 2002
BclI	TGATCA	2 cut(s)	517, 1897
BcnI	CCSGG	2 cut(s)	1356, 1846
BcoDI	GTCTC	3 cut(s)	74, 693, 1001
BfaI	CTAG	5 cut(s)	99, 648, 969, 1013, 1557
BfmI	CTRYAG	4 cut(s)	303, 924, 1023, 2590
BfoI	RGCGCY	1 cut(s)	1247
BfrI	CTTAAG	1 cut(s)	1666
BfuAI	ACCTGC	1 cut(s)	2104
BglII	AGATCT	2 cut(s)	559, 1909
BlnI	CCTAGG	1 cut(s)	1012
BlpI	GCTNAGC	1 cut(s)	1391
BmcAI	AGTACT	2 cut(s)	1180, 2607
Bme1390I	CCNGG	4 cut(s)	432, 1356, 1846, 2002
Bme18I	GGWCC	2 cut(s)	55, 2555
BmeT110I	CYCGRG	1 cut(s)	619
BmgT120I	GGNCC	3 cut(s)	55, 2332, 2555
BmiI	GGNNCC	6 cut(s)	57, 267, 763, 1591, 1640, 2334
BmrFI	CCNGG	4 cut(s)	432, 1356, 1846, 2002
BmrI	ACTGGG	1 cut(s)	1056
BmsI	GCATC	8 cut(s)	449, 483, 1418, 1550, 1661, 1686, 2016, 2080
BmuI	ACTGGG	1 cut(s)	1056
BpiI	GAAGAC	2 cut(s)	1211, 1343
BplI	GAGNNNNNCTC	2 cut(s)	530, 562
BpmI	CTGGAG	1 cut(s)	1524
Bpu1102I	GCTNAGC	1 cut(s)	1391
BpuEI	CTTGAG	1 cut(s)	365
BpuMI	CCSGG	2 cut(s)	1356, 1846
BsaAI	YACGTR	1 cut(s)	512
BsaHI	GRCGYC	1 cut(s)	9
BsaI	GGTCTC	2 cut(s)	74, 1001
BsaJI	CCNNGG	8 cut(s)	30, 430, 431, 774, 1012, 1845, 2001, 2127
BsaWI	WCCGGW	2 cut(s)	124, 2552
BsaXI	ACNNNNNCTCC	2 cut(s)	2484, 2514
Bsc4I	CCNNNNNNNGG	5 cut(s)	65, 842, 987, 1283, 1429
Bse118I	RCCGGY	1 cut(s)	2552
Bse1I	ACTGG	6 cut(s)	1051, 1186, 2077, 2334, 2464, 2583
Bse3DI	GCAATG	7 cut(s)	397, 992, 1239, 1258, 1585, 1976, 2073
BseAI	TCCGGA	1 cut(s)	124
BseBI	CCWGG	2 cut(s)	432, 2002
BseDI	CCNNGG	8 cut(s)	30, 430, 431, 774, 1012, 1845, 2001, 2127
BseGI	GGATG	7 cut(s)	464, 757, 1701, 1711, 1992, 2031, 2468
BseLI	CCNNNNNNNGG	5 cut(s)	65, 842, 987, 1283, 1429
BseMI	GCAATG	7 cut(s)	397, 992, 1239, 1258, 1585, 1976, 2073
BseMII	CTCAG	7 cut(s)	85, 95, 511, 689, 1137, 1614, 1929
BseNI	ACTGG	6 cut(s)	1051, 1186, 2077, 2334, 2464, 2583
BseRI	GAGGAG	1 cut(s)	362
BseSI	GKGCMC	1 cut(s)	1923
BseXI	GCAGC	7 cut(s)	215, 280, 289, 914, 1004, 2315, 2331
BseYI	CCCAGC	1 cut(s)	1916
BsgI	GTGCAG	2 cut(s)	1003, 2082
Bsh1236I	CGCG	3 cut(s)	357, 1407, 1830
BshFI	GGCC	4 cut(s)	773, 918, 2039, 2333
BshNI	GGYRCC	1 cut(s)	761
BshTI	ACCGGT	1 cut(s)	2552
BsiHKAI	GWGCWC	2 cut(s)	426, 1540
BsiHKCI	CYCGRG	1 cut(s)	619
BsiSI	CCGG	4 cut(s)	125, 1356, 1845, 2553
BslFI	GGGAC	3 cut(s)	413, 967, 1063
BslI	CCNNNNNNNGG	5 cut(s)	65, 842, 987, 1283, 1429
BsmAI	GTCTC	3 cut(s)	74, 693, 1001
BsmFI	GGGAC	3 cut(s)	413, 967, 1063
BsmI	GAATGC	2 cut(s)	982, 1770
BsnI	GGCC	4 cut(s)	773, 918, 2039, 2333
Bso31I	GGTCTC	2 cut(s)	74, 1001
BsoBI	CYCGRG	1 cut(s)	619
Bsp1286I	GDGCHC	3 cut(s)	426, 1540, 1923
Bsp13I	TCCGGA	1 cut(s)	124
Bsp1407I	TGTACA	1 cut(s)	1102
Bsp143I	GATC	9 cut(s)	20, 517, 559, 1474, 1738, 1771, 1897, 1909, 2262
Bsp1720I	GCTNAGC	1 cut(s)	1391
Bsp19I	CCATGG	1 cut(s)	2127
BspACI	CCGC	7 cut(s)	24, 690, 1140, 1693, 1879, 1932, 2204
BspANI	GGCC	4 cut(s)	773, 918, 2039, 2333
BspCNI	CTCAG	7 cut(s)	84, 94, 510, 688, 1136, 1615, 1930
BspEI	TCCGGA	1 cut(s)	124
BspFNI	CGCG	3 cut(s)	357, 1407, 1830
BspLI	GGNNCC	6 cut(s)	57, 267, 763, 1591, 1640, 2334
BspMAI	CTGCAG	2 cut(s)	307, 928
BspMI	ACCTGC	1 cut(s)	2104
BspPI	GGATC	3 cut(s)	15, 1746, 1766
BspT107I	GGYRCC	1 cut(s)	761
BspTI	CTTAAG	1 cut(s)	1666
BspTNI	GGTCTC	2 cut(s)	74, 1001
BsrDI	GCAATG	7 cut(s)	397, 992, 1239, 1258, 1585, 1976, 2073
BsrFI	RCCGGY	1 cut(s)	2552
BsrGI	TGTACA	1 cut(s)	1102
BsrI	ACTGG	6 cut(s)	1051, 1186, 2077, 2334, 2464, 2583
BssAI	RCCGGY	1 cut(s)	2552
BssECI	CCNNGG	8 cut(s)	30, 430, 431, 774, 1012, 1845, 2001, 2127
BssMI	GATC	9 cut(s)	20, 517, 559, 1474, 1738, 1771, 1897, 1909, 2262
BssNI	GRCGYC	1 cut(s)	9
BssSI	CACGAG	1 cut(s)	1470
BssT1I	CCWWGG	3 cut(s)	774, 1012, 2127
Bst2BI	CACGAG	1 cut(s)	1470
Bst2UI	CCWGG	2 cut(s)	432, 2002
Bst6I	CTCTTC	3 cut(s)	123, 1941, 2272
BstACI	GRCGYC	1 cut(s)	9
BstAFI	CTTAAG	1 cut(s)	1666
BstAPI	GCANNNNNTGC	3 cut(s)	302, 396, 584
BstAUI	TGTACA	1 cut(s)	1102
BstBAI	YACGTR	1 cut(s)	512
BstC8I	GCNNGC	5 cut(s)	787, 1397, 1822, 2379, 2508
BstDSI	CCRYGG	1 cut(s)	2127
BstF5I	GGATG	7 cut(s)	464, 757, 1701, 1711, 1992, 2031, 2468
BstFNI	CGCG	3 cut(s)	357, 1407, 1830
BstH2I	RGCGCY	1 cut(s)	1247
BstHHI	GCGC	4 cut(s)	359, 390, 1246, 1409
BstKTI	GATC	9 cut(s)	23, 520, 562, 1477, 1741, 1774, 1900, 1912, 2265
BstMAI	GTCTC	3 cut(s)	74, 693, 1001
BstMBI	GATC	9 cut(s)	20, 517, 559, 1474, 1738, 1771, 1897, 1909, 2262
BstNI	CCWGG	2 cut(s)	432, 2002
BstNSI	RCATGY	1 cut(s)	98
BstSCI	CCNGG	4 cut(s)	430, 1354, 1844, 2000
BstSFI	CTRYAG	4 cut(s)	303, 924, 1023, 2590
BstSLI	GKGCMC	1 cut(s)	1923
BstUI	CGCG	3 cut(s)	357, 1407, 1830
BstV1I	GCAGC	7 cut(s)	215, 280, 289, 914, 1004, 2315, 2331
BstV2I	GAAGAC	2 cut(s)	1211, 1343
BstX2I	RGATCY	4 cut(s)	20, 559, 1738, 1909
BstYI	RGATCY	4 cut(s)	20, 559, 1738, 1909
BsuRI	GGCC	4 cut(s)	773, 918, 2039, 2333
BtgI	CCRYGG	1 cut(s)	2127
BtsCI	GGATG	7 cut(s)	464, 757, 1701, 1711, 1992, 2031, 2468
BtsI	GCAGTG	2 cut(s)	573, 1042
BtsIMutI	CAGTG	6 cut(s)	79, 573, 1042, 1098, 1138, 2327
BveI	ACCTGC	1 cut(s)	2104
Cac8I	GCNNGC	5 cut(s)	787, 1397, 1822, 2379, 2508
CaiI	CAGNNNCTG	1 cut(s)	2519
CfoI	GCGC	4 cut(s)	359, 390, 1246, 1409
Cfr10I	RCCGGY	1 cut(s)	2552
Cfr13I	GGNCC	3 cut(s)	55, 2332, 2555
CseI	GACGC	1 cut(s)	17
Csp6I	GTAC	8 cut(s)	624, 762, 797, 1103, 1179, 1709, 2309, 2606
CspAI	ACCGGT	1 cut(s)	2552
CspCI	CAANNNNNGTGG	4 cut(s)	2182, 2217, 2371, 2406
CviAII	CATG	6 cut(s)	95, 842, 1400, 1809, 1813, 2128
CviQI	GTAC	8 cut(s)	624, 762, 797, 1103, 1179, 1709, 2309, 2606
DpnI	GATC	9 cut(s)	22, 519, 561, 1476, 1740, 1773, 1899, 1911, 2264
DpnII	GATC	9 cut(s)	20, 517, 559, 1474, 1738, 1771, 1897, 1909, 2262
DraI	TTTAAA	1 cut(s)	1444
EaeI	YGGCCR	1 cut(s)	771
Eam1104I	CTCTTC	3 cut(s)	123, 1941, 2272
EarI	CTCTTC	3 cut(s)	123, 1941, 2272
EciI	GGCGGA	1 cut(s)	13
Ecl136II	GAGCTC	1 cut(s)	424
Eco130I	CCWWGG	3 cut(s)	774, 1012, 2127
Eco147I	AGGCCT	2 cut(s)	918, 2039
Eco24I	GRGCYC	1 cut(s)	426
Eco31I	GGTCTC	2 cut(s)	74, 1001
Eco32I	GATATC	1 cut(s)	439
Eco47I	GGWCC	2 cut(s)	55, 2555
Eco47III	AGCGCT	1 cut(s)	1245
Eco53kI	GAGCTC	1 cut(s)	424
Eco57I	CTGAAG	5 cut(s)	933, 1179, 1224, 1335, 2145
Eco88I	CYCGRG	1 cut(s)	619
EcoICRI	GAGCTC	1 cut(s)	424
EcoRII	CCWGG	2 cut(s)	430, 2000
EcoRV	GATATC	1 cut(s)	439
EcoT14I	CCWWGG	3 cut(s)	774, 1012, 2127
EcoT22I	ATGCAT	1 cut(s)	1814
EcoT38I	GRGCYC	1 cut(s)	426
ErhI	CCWWGG	3 cut(s)	774, 1012, 2127
FaeI	CATG	6 cut(s)	98, 845, 1403, 1812, 1816, 2131
FaqI	GGGAC	3 cut(s)	413, 967, 1063
FatI	CATG	6 cut(s)	94, 841, 1399, 1808, 1812, 2127
FauNDI	CATATG	1 cut(s)	791
FbaI	TGATCA	2 cut(s)	517, 1897
FokI	GGATG	7 cut(s)	471, 764, 1708, 1718, 1999, 2038, 2475
FriOI	GRGCYC	1 cut(s)	426
FspBI	CTAG	5 cut(s)	99, 648, 969, 1013, 1557
FspI	TGCGCA	1 cut(s)	389
GlaI	GCGC	4 cut(s)	358, 389, 1245, 1408
GsaI	CCCAGC	1 cut(s)	1920
GsuI	CTGGAG	1 cut(s)	1524
HaeII	RGCGCY	1 cut(s)	1247
HaeIII	GGCC	4 cut(s)	773, 918, 2039, 2333
HapII	CCGG	4 cut(s)	125, 1356, 1845, 2553
HgaI	GACGC	1 cut(s)	17
HhaI	GCGC	4 cut(s)	359, 390, 1246, 1409
Hin1I	GRCGYC	1 cut(s)	9
Hin1II	CATG	6 cut(s)	98, 845, 1403, 1812, 1816, 2131
Hin6I	GCGC	4 cut(s)	357, 388, 1244, 1407
HinP1I	GCGC	4 cut(s)	357, 388, 1244, 1407
HincII	GTYRAC	2 cut(s)	1311, 2356
HindII	GTYRAC	2 cut(s)	1311, 2356
HpaII	CCGG	4 cut(s)	125, 1356, 1845, 2553
HphI	GGTGA	6 cut(s)	548, 1834, 1990, 2087, 2291, 2497
Hpy166II	GTNNAC	4 cut(s)	55, 797, 1311, 2356
Hpy8I	GTNNAC	4 cut(s)	55, 797, 1311, 2356
HpyAV	CCTTC	9 cut(s)	279, 908, 929, 1154, 1729, 2022, 2101, 2214, 2416
HpyCH4IV	ACGT	2 cut(s)	117, 511
HpySE526I	ACGT	2 cut(s)	117, 511
Hsp92I	GRCGYC	1 cut(s)	9
Hsp92II	CATG	6 cut(s)	98, 845, 1403, 1812, 1816, 2131
HspAI	GCGC	4 cut(s)	357, 388, 1244, 1407
Kpn2I	TCCGGA	1 cut(s)	124
KpnI	GGTACC	1 cut(s)	765
Ksp22I	TGATCA	2 cut(s)	517, 1897
Kzo9I	GATC	9 cut(s)	20, 517, 559, 1474, 1738, 1771, 1897, 1909, 2262
LmnI	GCTCC	6 cut(s)	265, 349, 1287, 1543, 1595, 2492
Lsp1109I	GCAGC	7 cut(s)	215, 280, 289, 914, 1004, 2315, 2331
LweI	GCATC	8 cut(s)	449, 483, 1418, 1550, 1661, 1686, 2016, 2080
MaeI	CTAG	5 cut(s)	99, 648, 969, 1013, 1557
MaeII	ACGT	2 cut(s)	117, 511
MalI	GATC	9 cut(s)	22, 519, 561, 1476, 1740, 1773, 1899, 1911, 2264
MboI	GATC	9 cut(s)	20, 517, 559, 1474, 1738, 1771, 1897, 1909, 2262
MboII	GAAGA	9 cut(s)	140, 731, 1211, 1214, 1343, 1354, 1368, 1958, 2259
MfeI	CAATTG	2 cut(s)	1208, 2388
MflI	RGATCY	4 cut(s)	20, 559, 1738, 1909
MhlI	GDGCHC	3 cut(s)	426, 1540, 1923
MlsI	TGGCCA	1 cut(s)	773
MluI	ACGCGT	1 cut(s)	1828
MluNI	TGGCCA	1 cut(s)	773
MlyI	GAGTC	4 cut(s)	695, 1045, 1316, 1708
MmeI	TCCRAC	4 cut(s)	18, 649, 1608, 1741
Mox20I	TGGCCA	1 cut(s)	773
Mph1103I	ATGCAT	1 cut(s)	1814
MroI	TCCGGA	1 cut(s)	124
MroXI	GAANNNNTTC	1 cut(s)	918
MscI	TGGCCA	1 cut(s)	773
MslI	CAYNNNNRTG	5 cut(s)	32, 828, 1232, 1728, 2052
Msp20I	TGGCCA	1 cut(s)	773
MspA1I	CMGCKG	5 cut(s)	1140, 1865, 2144, 2306, 2344
MspCI	CTTAAG	1 cut(s)	1666
MspI	CCGG	4 cut(s)	125, 1356, 1845, 2553
MspR9I	CCNGG	4 cut(s)	432, 1356, 1846, 2002
MunI	CAATTG	2 cut(s)	1208, 2388
Mva1269I	GAATGC	2 cut(s)	982, 1770
MvaI	CCWGG	2 cut(s)	432, 2002
MvnI	CGCG	3 cut(s)	357, 1407, 1830
NciI	CCSGG	2 cut(s)	1356, 1846
NcoI	CCATGG	1 cut(s)	2127
NdeI	CATATG	1 cut(s)	791
NdeII	GATC	9 cut(s)	20, 517, 559, 1474, 1738, 1771, 1897, 1909, 2262
NlaIII	CATG	6 cut(s)	98, 845, 1403, 1812, 1816, 2131
NlaIV	GGNNCC	6 cut(s)	57, 267, 763, 1591, 1640, 2334
NmuCI	GTSAC	6 cut(s)	74, 727, 821, 830, 1037, 1840
NsbI	TGCGCA	1 cut(s)	389
NsiI	ATGCAT	1 cut(s)	1814
NspI	RCATGY	1 cut(s)	98
OliI	CACNNNNGTG	2 cut(s)	32, 828
PaqCI	CACCTGC	1 cut(s)	2104
PasI	CCCWGGG	1 cut(s)	431
PceI	AGGCCT	2 cut(s)	918, 2039
PctI	GAATGC	2 cut(s)	982, 1770
PdmI	GAANNNNTTC	1 cut(s)	918
PfeI	GAWTC	9 cut(s)	566, 849, 1256, 1364, 1435, 1631, 1868, 2408, 2586
PinAI	ACCGGT	1 cut(s)	2552
PleI	GAGTC	4 cut(s)	695, 1045, 1315, 1708
PpsI	GAGTC	4 cut(s)	695, 1045, 1315, 1708
Ppu21I	YACGTR	1 cut(s)	512
PshBI	ATTAAT	1 cut(s)	234
Psp124BI	GAGCTC	1 cut(s)	426
Psp6I	CCWGG	2 cut(s)	430, 2000
PspFI	CCCAGC	1 cut(s)	1916
PspGI	CCWGG	2 cut(s)	430, 2000
PspN4I	GGNNCC	6 cut(s)	57, 267, 763, 1591, 1640, 2334
PspPI	GGNCC	3 cut(s)	55, 2332, 2555
PstI	CTGCAG	2 cut(s)	307, 928
PstNI	CAGNNNCTG	1 cut(s)	2519
PsuI	RGATCY	4 cut(s)	20, 559, 1738, 1909
PvuII	CAGCTG	4 cut(s)	1865, 2144, 2306, 2344
RsaI	GTAC	8 cut(s)	625, 763, 798, 1104, 1180, 1710, 2310, 2607
RsaNI	GTAC	8 cut(s)	624, 762, 797, 1103, 1179, 1709, 2309, 2606
RseI	CAYNNNNRTG	5 cut(s)	32, 828, 1232, 1728, 2052
SacI	GAGCTC	1 cut(s)	426
Sau3AI	GATC	9 cut(s)	20, 517, 559, 1474, 1738, 1771, 1897, 1909, 2262
Sau96I	GGNCC	3 cut(s)	55, 2332, 2555
ScaI	AGTACT	2 cut(s)	1180, 2607
SchI	GAGTC	4 cut(s)	695, 1045, 1316, 1708
ScrFI	CCNGG	4 cut(s)	432, 1356, 1846, 2002
SduI	GDGCHC	3 cut(s)	426, 1540, 1923
SfaNI	GCATC	8 cut(s)	449, 483, 1418, 1550, 1661, 1686, 2016, 2080
SfcI	CTRYAG	4 cut(s)	303, 924, 1023, 2590
SinI	GGWCC	2 cut(s)	55, 2555
SmiMI	CAYNNNNRTG	5 cut(s)	32, 828, 1232, 1728, 2052
SmlI	CTYRAG	2 cut(s)	344, 1666
SmoI	CTYRAG	2 cut(s)	344, 1666
SseBI	AGGCCT	2 cut(s)	918, 2039
SsiI	CCGC	7 cut(s)	24, 690, 1140, 1693, 1879, 1932, 2204
SspI	AATATT	3 cut(s)	610, 1570, 2275
SspMI	CTAG	5 cut(s)	99, 648, 969, 1013, 1557
SstI	GAGCTC	1 cut(s)	426
StuI	AGGCCT	2 cut(s)	918, 2039
StyD4I	CCNGG	4 cut(s)	430, 1354, 1844, 2000
StyI	CCWWGG	3 cut(s)	774, 1012, 2127
TaiI	ACGT	2 cut(s)	120, 514
TaqI	TCGA	1 cut(s)	2007
TatI	WGTACW	5 cut(s)	796, 1102, 1178, 2308, 2605
TauI	GCSGC	3 cut(s)	693, 1881, 2207
TfiI	GAWTC	9 cut(s)	566, 849, 1256, 1364, 1435, 1631, 1868, 2408, 2586
TscAI	CASTG	6 cut(s)	79, 580, 1042, 1105, 1138, 2334
TseFI	GTSAC	6 cut(s)	74, 727, 821, 830, 1037, 1840
TseI	GCWGC	7 cut(s)	203, 293, 302, 902, 1017, 2303, 2344
Tsp45I	GTSAC	6 cut(s)	74, 727, 821, 830, 1037, 1840
TspGWI	ACGGA	3 cut(s)	731, 1316, 1999
TspRI	CASTG	6 cut(s)	79, 580, 1042, 1105, 1138, 2334
Vha464I	CTTAAG	1 cut(s)	1666
VpaK11BI	GGWCC	2 cut(s)	55, 2555
VspI	ATTAAT	1 cut(s)	234
XapI	RAATTY	4 cut(s)	465, 755, 1196, 1384
XbaI	TCTAGA	1 cut(s)	1556
XceI	RCATGY	1 cut(s)	98
XcmI	CCANNNNNNNNNTGG	1 cut(s)	34
XmaJI	CCTAGG	1 cut(s)	1012
XmnI	GAANNNNTTC	1 cut(s)	918
XspI	CTAG	5 cut(s)	99, 648, 969, 1013, 1557
ZrmI	AGTACT	2 cut(s)	1180, 2607
Zsp2I	ATGCAT	1 cut(s)	1814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.