Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008061

Physical Location & Seq

Genomic Coordinates Reverse (-)

6669712 .. 6686775

Gene Structure 17064 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1080200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3009 bp

ATGGAGGACGCTTACGCTCGATCCGTCACCGAGGTGTTGGATTTCTTCGGCGTGGACCCGAAGAAGGGCCTCAATGACTCACAGGTTGCGCAACATGCAAGACTGTACGGCAAAAATGTGCTTCCGGAAGAGAAAAGGGCTTCATTCTGGAAATTGGTTTTGAAACAGTTTGATGATTTGCTTGTAAAGATCTTGATTGTTGCGGCGCTTGTTTCTTTTGTTCTGGCTTTGATTAATGGAGATACGGGATTAACAGCTTTTTTGGAGCCTTCTGTTATTCTCTTGATATTGGCTGCAAATGCTGCAGTAGGAGTGATTACAGAAACAAATGCAGAAAAGGCTCTTGAGGAGCTACGTGCATACCAAGCTGATATTGCAACTGTGCTGCGGAATGGTTGCTTCTCCATACTTCCAGCCACAGAGCTTGTCCCAGGTGATATTGTAGAAGTGGCTGTGGGAGGCAAAATTCCAGCTGATATGAGAATGATTGAGATGCTAAGTAATCAATTACGTGTTGATCAGGCAATTCTTACAGGTGAGAGTTGCTCTGTCGGAAAAGAGCTTGAGTCCACCACTGCAACAAATGTTGTATATCAAGACAAGACAAATATTCTTTTTTCGGGTACAGTAGTCGTGGCTGGTAGGGCTAGAGCTGTTGTAGTAGGAGTTGGAACACACACTGCTATGGGCGGCATACATGATTCAATGTTGCAAACGGAAGATGAAGTGACGCCATTGAAAAAGAAGCTGGACGAATTTGGTACATTCTTGGCCAAGGTTATTGCTGGCATATGTGTACTGGTATGGATTGTAAATATTGGTCACTTTCGTGACCCTGCTCATGGTGGGTTCTTGCGTGGTGCAATTCATTACTTCAAGATTGCAGTCGCACTTGCAGTTGCAGCAATCCCTGAAGGACTTCCAGCTGTCGTTACAACTTGTTTGGCTCTTGGGACAAAACGAATGGCTCGGTTGAATGCCATTGTAAGGTCTTTGCCATCTGTTGAGACTTTAGGCTGCACTACAGTAATCTGCAGTGACAAAACCGGAACACTGACAACCAATATGATGTCTGCCTCAAAGGTTTGTGTGCTTCATACTGTACAACATGCTCCTGTTATTTCTGAATACAGTGTCAGTGGAACAACATACGCACCAGAGGGCTTAATTTTTTACAGTACTGGGCTTCAGCTTGAACTTCCAGCTCAGTCTCCTTGTCTTCTTCACATAGCAATGTGTTCAGCACTATGCAATGAGTCTATCCTGCAATATAATCCTGACAAGAGTAACTATGAAAAAATTGGCGAGTCAACTGAAGTTGCCCTCCGTGTTCTTGCAGAGAAGATTGGTCTTCCTGGCTTTGATTCTATGCCTTCTTCTCTGAATATGCTGAGCAAGCATGAACGTGCGTCTTACTGTAATCACTACTGGGAGGATCATTTTAAAAAGATTTCTGTTGCAGATTTTACTCGTGATCGGAAGATGATGAGTGTTCTTTGCAGCCGAAACCAATTGCAAATTATGTTTTCAAAAGGTGCTCCAGAGAGTATTATTTCTAGATGCACCAGTATTCTGTGCAATGATGATGGTTCTGCCATACCACTAACCACTAGTATCCGGGCTGAGCTGGAATCAAGGTTCCATAGTTTTGCAGGAAAGGAAACACTAAGATGCTTGGCCCTGGCCTTCAAGCGGATGCCCATGGGTCTACAGAGTCTCTCCCATAAAGATGAGAAGGATCTAACATTCATTGGGTTGGTTGGAATGCTTGATCCACCAAGAGAAGAAGTGAGAAATGCTATGCTTTCATGCATGACTGCTGGCATACGAGTTATTGTTGTAACTGGGGACAACAAGTCAACAGCTGAATCACTTTGCCGCAAGATAGGGGCTTTTGATCACTTAGAAGATCTTGCTGGACACTCTTACACTGCAACTGAGTTTGAAGAATTACCAGCAATGCAGAAAACGCTGGCATTGCAACGCATGGCACTATTCACTAGAGTTGAACCTTCTCATAAAAGGATGCTGGTTGAGGCCTTGCGAAACCAGAACGAAGTGGTTGCAATGACCGGTGATGGTGTTAATGATGCACCTGCACTAAAAAAGGCAGATATTGGAATTGCCATGGGTTCAGGAACAGCCGTTGCCAAGAGTGCTTCAGATATGGTTTTGGCAGATGACAATTTCGCTACTATTGTTGCAGCTGTTGCAGAGGGAAGGGCTATATACAATAACACAAAGCAATTTATCAGATATATGATCTCTTCAAATATTGGTGAAGTAGTTTGTATATTTGTGGCAGCTGTACTTGGCATACCTGATACCCTAGCCCCTGTCCAGCTGCTCTGGGTCAACTTAGTTACTGATGGATTACCTGCCACTGCTATTGGTTTCAATAAGCAAGATTCTGATGTGATGAAAGCTAAACCTCGTAAGGTCAATGAAGCAGTTGTTTCTGGATGGCTGTTTTTTCGTTATTTGGTAATTGGAGCTTATGTTGGGCTTGCCACGGTTGCCGGATTTATATGGTGGTTTGTTTACTCTGATAACGGTCCTCAACTGCCATATTCTGAATTGATGAATTTTGACTCATGCACAACAAGGGATACAACTTATCCATGCAGTATATTTGATGACCGCCATCCATCAACTGTATCCATGACTGTGCTTGTGGTTGTTGAAATGTTTAACGCCTTGAATAATCTCAGTGAAAATCAGTCTCTTGTTGTTATCCCTCCGTGGAGTAACTTGTGGCTTGTTGGTTCGATTATTCTAACGATGATCCTTCACATACTAATATTATACGTCCATCCGCTCTCTATTCTTTTCTCCGTTACACCATTATCTTGGGCTGACTGGACTGTTGTGCTATACCTTTCATTTCCCGTTATTATCATCGATGAAGTGCTAAAGTTCTTATCAAGAAGTTCTACTGGCATGCGGTGGTTCAATCTCCGGTGGAGGCGGCCTGATTTTCTGCCAAAAAGGGAATCACGTGACAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0003824 GO:0005215 GO:0005384 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006828 GO:0007275 GO:0008150 GO:0008324 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015410 GO:0015662 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022622 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055071 GO:0055076 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071421 GO:0072511 GO:0090662 GO:0097708 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0099131 GO:0099132 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04020 ko04022 ko04972 ko05010 map04020 map04022 map04972 map05010

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1003

Amino Acids

109.71

Weight (kDa)

6.04

Isoelectric Point (pI)

40.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	5 - 72	2.6e-20	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	122 - 235	1.6e-22	P-type ATPase actuator domain
Cation_ATPase	PF13246	409 - 596	2.8e-40	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	576 - 705	9.4e-14	haloacid dehalogenase-like hydrolase
Hydrolase_3	PF08282	677 - 732	8.4e-06	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	776 - 974	5.7e-45	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10130
fragaria_vesca	FvH4_2g32870 FvH4_2g32870 FvH4_2g32870
malus_domestica	MD08G1080200.v1.1 MD15G1067300.v1.1
prunus_persica	Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1
pyrus_communis	pycom08g06620 pycom15g06380
rosa_chinensis	RchiOBHm_Chr6g0309841
rosa_laevigata	RLG00000010530
rosa_multiflora	Rmu_ssc0000373.1_g000002
rosa_roxburghii	Rroxscaffold_7G00159130
rosa_rugosa	Rorug06G0375200 Rorug06G0375300
rosa_samantha	Rh6AG488700 Rh6BG498200 Rh6CG503000 Rh6DG489200
rosa_wichuraiana	Rw6G042560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2104
Acc16I	TGCGCA	1 cut(s)	90
Acc36I	ACCTGC	2 cut(s)	2104, 2386
AccBSI	CCGCTC	1 cut(s)	2818
AccI	GTMKAC	1 cut(s)	1708
AccIII	TCCGGA	1 cut(s)	124
AciI	CCGC	9 cut(s)	203, 388, 690, 1693, 1879, 2641, 2816, 2944, 2968
AclWI	GGATC	5 cut(s)	15, 1443, 1746, 1766, 2779
AcoI	YGGCCR	1 cut(s)	771
AcsI	RAATTY	3 cut(s)	465, 755, 2584
AcuI	CTGAAG	4 cut(s)	933, 1172, 1335, 2145
AcvI	CACGTG	1 cut(s)	2999
AcyI	GRCGYC	1 cut(s)	731
AfaI	GTAC	7 cut(s)	107, 625, 763, 798, 1104, 1180, 2310
AfiI	CCNNNNNNNGG	5 cut(s)	64, 65, 842, 987, 1692
AflIII	ACRYGT	1 cut(s)	511
AgeI	ACCGGT	1 cut(s)	2072
AhdI	GACNNNNNGTC	1 cut(s)	1855
AhlI	ACTAGT	1 cut(s)	1610
AjnI	CCWGG	3 cut(s)	430, 1354, 1680
AleI	CACNNNNGTG	2 cut(s)	32, 828
AloI	GAACNNNNNNTCC	2 cut(s)	1622, 1654
Alw21I	GWGCWC	1 cut(s)	1540
Alw26I	GTCTC	4 cut(s)	1001, 1215, 1721, 2727
AlwI	GGATC	5 cut(s)	15, 1443, 1746, 1766, 2779
Aor13HI	TCCGGA	1 cut(s)	124
AoxI	GGCC	6 cut(s)	67, 771, 1677, 1683, 2037, 2969
ApeKI	GCWGC	9 cut(s)	293, 302, 385, 902, 1017, 1500, 2204, 2303, 2344
ApoI	RAATTY	3 cut(s)	465, 755, 2584
AseI	ATTAAT	1 cut(s)	234
AsiGI	ACCGGT	1 cut(s)	2072
Asp700I	GAANNNNTTC	1 cut(s)	918
AspLEI	GCGC	2 cut(s)	91, 208
AspS9I	GGNCC	4 cut(s)	55, 67, 1678, 2555
AsuC2I	CCSGG	1 cut(s)	1619
AsuHPI	GGTGA	5 cut(s)	19, 446, 548, 2087, 2291
AvaII	GGWCC	2 cut(s)	55, 2555
BaeI	ACNNNNGTAYC	2 cut(s)	615, 648
BalI	TGGCCA	1 cut(s)	773
BauI	CACGAG	1 cut(s)	1470
BbrPI	CACGTG	1 cut(s)	2999
BbsI	GAAGAC	2 cut(s)	1211, 1343
Bbv12I	GWGCWC	1 cut(s)	1540
BbvI	GCAGC	9 cut(s)	280, 289, 372, 914, 1004, 1512, 2216, 2315, 2331
BccI	CCATC	8 cut(s)	1006, 1580, 2072, 2363, 2457, 2652, 2656, 2820
BceAI	ACGGC	2 cut(s)	124, 2129
BcgI	CGANNNNNNTGC	2 cut(s)	2045, 2079
BciT130I	CCWGG	3 cut(s)	432, 1356, 1682
BciVI	GTATCC	3 cut(s)	1625, 2602, 2668
BclI	TGATCA	2 cut(s)	517, 1897
BcnI	CCSGG	1 cut(s)	1619
BcoDI	GTCTC	4 cut(s)	1001, 1215, 1721, 2727
BcuI	ACTAGT	1 cut(s)	1610
BfaI	CTAG	5 cut(s)	648, 1557, 1611, 2001, 2330
BfmI	CTRYAG	4 cut(s)	303, 1023, 1033, 1709
BfoI	RGCGCY	1 cut(s)	209
BfuAI	ACCTGC	2 cut(s)	2104, 2386
BfuI	GTATCC	3 cut(s)	1625, 2602, 2668
BglII	AGATCT	2 cut(s)	189, 1909
BlpI	GCTNAGC	2 cut(s)	1391, 1623
BmcAI	AGTACT	1 cut(s)	1180
Bme1390I	CCNGG	4 cut(s)	432, 1356, 1619, 1682
Bme18I	GGWCC	2 cut(s)	55, 2555
BmeRI	GACNNNNNGTC	1 cut(s)	1855
BmgT120I	GGNCC	4 cut(s)	55, 67, 1678, 2555
BmiI	GGNNCC	3 cut(s)	57, 267, 1640
BmrFI	CCNGG	4 cut(s)	432, 1356, 1619, 1682
BmrI	ACTGGG	3 cut(s)	1191, 1438, 1854
BmsI	GCATC	6 cut(s)	483, 1550, 1661, 1686, 2016, 2080
BmuI	ACTGGG	3 cut(s)	1191, 1438, 1854
BpiI	GAAGAC	2 cut(s)	1211, 1343
BplI	GAGNNNNNCTC	2 cut(s)	530, 562
BpmI	CTGGAG	1 cut(s)	1524
Bpu1102I	GCTNAGC	2 cut(s)	1391, 1623
BpuEI	CTTGAG	2 cut(s)	365, 584
BpuMI	CCSGG	1 cut(s)	1619
Bsa29I	ATCGAT	1 cut(s)	2901
BsaAI	YACGTR	3 cut(s)	356, 512, 2999
BsaHI	GRCGYC	1 cut(s)	731
BsaJI	CCNNGG	8 cut(s)	30, 430, 774, 1680, 1701, 2127, 2511, 2741
BsaWI	WCCGGW	4 cut(s)	124, 1046, 2072, 2958
BsaXI	ACNNNNNCTCC	2 cut(s)	2484, 2514
Bsc4I	CCNNNNNNNGG	5 cut(s)	64, 65, 842, 987, 1692
Bse118I	RCCGGY	1 cut(s)	2072
Bse1I	ACTGG	7 cut(s)	804, 1186, 1433, 1566, 1849, 2864, 2941
Bse3DI	GCAATG	6 cut(s)	1239, 1258, 1585, 1965, 1976, 2073
BseAI	TCCGGA	1 cut(s)	124
BseBI	CCWGG	3 cut(s)	432, 1356, 1682
BseCI	ATCGAT	1 cut(s)	2901
BseDI	CCNNGG	8 cut(s)	30, 430, 774, 1680, 1701, 2127, 2511, 2741
BseGI	GGATG	5 cut(s)	1701, 2031, 2468, 2644, 2812
BseLI	CCNNNNNNNGG	5 cut(s)	64, 65, 842, 987, 1692
BseMI	GCAATG	6 cut(s)	1239, 1258, 1585, 1965, 1976, 2073
BseMII	CTCAG	5 cut(s)	1220, 1382, 1614, 1929, 2722
BseNI	ACTGG	7 cut(s)	804, 1186, 1433, 1566, 1849, 2864, 2941
BseRI	GAGGAG	1 cut(s)	362
BseXI	GCAGC	9 cut(s)	280, 289, 372, 914, 1004, 1512, 2216, 2315, 2331
BsgI	GTGCAG	2 cut(s)	1003, 2082
BshFI	GGCC	6 cut(s)	69, 773, 1679, 1685, 2039, 2971
BshTI	ACCGGT	1 cut(s)	2072
BshVI	ATCGAT	1 cut(s)	2901
BsiHKAI	GWGCWC	1 cut(s)	1540
BsiSI	CCGG	6 cut(s)	125, 1047, 1618, 2073, 2520, 2959
BslFI	GGGAC	3 cut(s)	413, 967, 1862
BslI	CCNNNNNNNGG	5 cut(s)	64, 65, 842, 987, 1692
BsmAI	GTCTC	4 cut(s)	1001, 1215, 1721, 2727
BsmFI	GGGAC	3 cut(s)	413, 967, 1862
BsmI	GAATGC	2 cut(s)	982, 1770
BsnI	GGCC	6 cut(s)	69, 773, 1679, 1685, 2039, 2971
Bsp1286I	GDGCHC	1 cut(s)	1540
Bsp13I	TCCGGA	1 cut(s)	124
Bsp1407I	TGTACA	1 cut(s)	1102
Bsp1720I	GCTNAGC	2 cut(s)	1391, 1623
Bsp19I	CCATGG	2 cut(s)	1701, 2127
BspACI	CCGC	9 cut(s)	203, 388, 690, 1693, 1879, 2641, 2816, 2944, 2968
BspANI	GGCC	6 cut(s)	69, 773, 1679, 1685, 2039, 2971
BspCNI	CTCAG	5 cut(s)	1219, 1383, 1615, 1930, 2721
BspDI	ATCGAT	1 cut(s)	2901
BspEI	TCCGGA	1 cut(s)	124
BspLI	GGNNCC	3 cut(s)	57, 267, 1640
BspMAI	CTGCAG	2 cut(s)	307, 1037
BspMI	ACCTGC	2 cut(s)	2104, 2386
BspPI	GGATC	5 cut(s)	15, 1443, 1746, 1766, 2779
BsrBI	CCGCTC	1 cut(s)	2818
BsrDI	GCAATG	6 cut(s)	1239, 1258, 1585, 1965, 1976, 2073
BsrFI	RCCGGY	1 cut(s)	2072
BsrGI	TGTACA	1 cut(s)	1102
BsrI	ACTGG	7 cut(s)	804, 1186, 1433, 1566, 1849, 2864, 2941
BssAI	RCCGGY	1 cut(s)	2072
BssECI	CCNNGG	8 cut(s)	30, 430, 774, 1680, 1701, 2127, 2511, 2741
BssNI	GRCGYC	1 cut(s)	731
BssSI	CACGAG	1 cut(s)	1470
BssT1I	CCWWGG	3 cut(s)	774, 1701, 2127
Bst2BI	CACGAG	1 cut(s)	1470
Bst2UI	CCWGG	3 cut(s)	432, 1356, 1682
Bst6I	CTCTTC	2 cut(s)	123, 2272
BstACI	GRCGYC	1 cut(s)	731
BstAPI	GCANNNNNTGC	2 cut(s)	302, 2210
BstAUI	TGTACA	1 cut(s)	1102
BstBAI	YACGTR	3 cut(s)	356, 512, 2999
BstC8I	GCNNGC	6 cut(s)	787, 1397, 1822, 1974, 2508, 2942
BstDEI	CTNAG	9 cut(s)	497, 1206, 1391, 1623, 1667, 1903, 1938, 2359, 2708
BstDSI	CCRYGG	4 cut(s)	1701, 2127, 2511, 2741
BstF5I	GGATG	5 cut(s)	1701, 2031, 2468, 2644, 2812
BstH2I	RGCGCY	1 cut(s)	209
BstHHI	GCGC	2 cut(s)	91, 208
BstMAI	GTCTC	4 cut(s)	1001, 1215, 1721, 2727
BstNI	CCWGG	3 cut(s)	432, 1356, 1682
BstNSI	RCATGY	3 cut(s)	98, 1112, 2944
BstSCI	CCNGG	4 cut(s)	430, 1354, 1617, 1680
BstSFI	CTRYAG	4 cut(s)	303, 1023, 1033, 1709
BstV1I	GCAGC	9 cut(s)	280, 289, 372, 914, 1004, 1512, 2216, 2315, 2331
BstV2I	GAAGAC	2 cut(s)	1211, 1343
BstX2I	RGATCY	3 cut(s)	189, 1738, 1909
BstXI	CCANNNNNNTGG	1 cut(s)	2850
BstYI	RGATCY	3 cut(s)	189, 1738, 1909
Bsu15I	ATCGAT	1 cut(s)	2901
BsuI	GTATCC	3 cut(s)	1625, 2602, 2668
BsuRI	GGCC	6 cut(s)	69, 773, 1679, 1685, 2039, 2971
BsuTUI	ATCGAT	1 cut(s)	2901
BtgI	CCRYGG	4 cut(s)	1701, 2127, 2511, 2741
BtsCI	GGATG	5 cut(s)	1701, 2031, 2468, 2644, 2812
BtsI	GCAGTG	5 cut(s)	573, 678, 1042, 1929, 2382
BtsIMutI	CAGTG	9 cut(s)	573, 678, 1042, 1052, 1138, 1144, 1929, 2382, 2716
BveI	ACCTGC	2 cut(s)	2104, 2386
Cac8I	GCNNGC	6 cut(s)	787, 1397, 1822, 1974, 2508, 2942
CfoI	GCGC	2 cut(s)	91, 208
Cfr10I	RCCGGY	1 cut(s)	2072
Cfr13I	GGNCC	4 cut(s)	55, 67, 1678, 2555
ClaI	ATCGAT	1 cut(s)	2901
CseI	GACGC	3 cut(s)	17, 739, 1398
Csp6I	GTAC	7 cut(s)	106, 624, 762, 797, 1103, 1179, 2309
CspAI	ACCGGT	1 cut(s)	2072
CspCI	CAANNNNNGTGG	4 cut(s)	406, 441, 2371, 2406
CviQI	GTAC	7 cut(s)	106, 624, 762, 797, 1103, 1179, 2309
DdeI	CTNAG	9 cut(s)	497, 1206, 1391, 1623, 1667, 1903, 1938, 2359, 2708
DraI	TTTAAA	1 cut(s)	1444
DriI	GACNNNNNGTC	1 cut(s)	1855
EaeI	YGGCCR	1 cut(s)	771
Eam1104I	CTCTTC	2 cut(s)	123, 2272
Eam1105I	GACNNNNNGTC	1 cut(s)	1855
EarI	CTCTTC	2 cut(s)	123, 2272
Eco130I	CCWWGG	3 cut(s)	774, 1701, 2127
Eco147I	AGGCCT	1 cut(s)	2039
Eco47I	GGWCC	2 cut(s)	55, 2555
Eco57I	CTGAAG	4 cut(s)	933, 1172, 1335, 2145
Eco72I	CACGTG	1 cut(s)	2999
EcoO109I	RGGNCCY	1 cut(s)	67
EcoRII	CCWGG	3 cut(s)	430, 1354, 1680
EcoT14I	CCWWGG	3 cut(s)	774, 1701, 2127
EcoT22I	ATGCAT	1 cut(s)	1814
ErhI	CCWWGG	3 cut(s)	774, 1701, 2127
FaqI	GGGAC	3 cut(s)	413, 967, 1862
FauNDI	CATATG	1 cut(s)	791
FbaI	TGATCA	2 cut(s)	517, 1897
FblI	GTMKAC	1 cut(s)	1708
FokI	GGATG	5 cut(s)	1708, 2038, 2475, 2631, 2799
FspBI	CTAG	5 cut(s)	648, 1557, 1611, 2001, 2330
FspI	TGCGCA	1 cut(s)	90
GlaI	GCGC	2 cut(s)	90, 207
GsuI	CTGGAG	1 cut(s)	1524
HaeII	RGCGCY	1 cut(s)	209
HaeIII	GGCC	6 cut(s)	69, 773, 1679, 1685, 2039, 2971
HapII	CCGG	6 cut(s)	125, 1047, 1618, 2073, 2520, 2959
HgaI	GACGC	3 cut(s)	17, 739, 1398
HhaI	GCGC	2 cut(s)	91, 208
Hin1I	GRCGYC	1 cut(s)	731
Hin6I	GCGC	2 cut(s)	89, 206
HinP1I	GCGC	2 cut(s)	89, 206
HincII	GTYRAC	3 cut(s)	1311, 1860, 2356
HindII	GTYRAC	3 cut(s)	1311, 1860, 2356
HpaII	CCGG	6 cut(s)	125, 1047, 1618, 2073, 2520, 2959
HphI	GGTGA	5 cut(s)	19, 446, 548, 2087, 2291
Hpy166II	GTNNAC	8 cut(s)	55, 570, 797, 1311, 1709, 1860, 2356, 2542
Hpy188I	TCNGA	9 cut(s)	554, 1126, 1383, 1479, 2164, 2255, 2413, 2548, 2575
Hpy8I	GTNNAC	8 cut(s)	55, 570, 797, 1311, 1709, 1860, 2356, 2542
HpyAV	CCTTC	9 cut(s)	58, 279, 908, 1383, 1696, 1729, 2022, 2214, 2798
HpyCH4IV	ACGT	5 cut(s)	355, 511, 1405, 2808, 2998
HpyF3I	CTNAG	9 cut(s)	497, 1206, 1391, 1623, 1667, 1903, 1938, 2359, 2708
HpySE526I	ACGT	5 cut(s)	355, 511, 1405, 2808, 2998
Hsp92I	GRCGYC	1 cut(s)	731
HspAI	GCGC	2 cut(s)	89, 206
Kpn2I	TCCGGA	1 cut(s)	124
Ksp22I	TGATCA	2 cut(s)	517, 1897
LmnI	GCTCC	5 cut(s)	265, 349, 1117, 1543, 2492
Lsp1109I	GCAGC	9 cut(s)	280, 289, 372, 914, 1004, 1512, 2216, 2315, 2331
LweI	GCATC	6 cut(s)	483, 1550, 1661, 1686, 2016, 2080
MaeI	CTAG	5 cut(s)	648, 1557, 1611, 2001, 2330
MaeII	ACGT	5 cut(s)	355, 511, 1405, 2808, 2998
MbiI	CCGCTC	1 cut(s)	2818
MfeI	CAATTG	1 cut(s)	1511
MflI	RGATCY	3 cut(s)	189, 1738, 1909
MhlI	GDGCHC	1 cut(s)	1540
MlsI	TGGCCA	1 cut(s)	773
MluNI	TGGCCA	1 cut(s)	773
MlyI	GAGTC	6 cut(s)	71, 575, 1265, 1316, 1723, 2585
MmeI	TCCRAC	4 cut(s)	18, 532, 649, 1741
Mox20I	TGGCCA	1 cut(s)	773
Mph1103I	ATGCAT	1 cut(s)	1814
MroI	TCCGGA	1 cut(s)	124
MroXI	GAANNNNTTC	1 cut(s)	918
MscI	TGGCCA	1 cut(s)	773
MseI	TTAA	6 cut(s)	234, 251, 1166, 1443, 2085, 2691
MslI	CAYNNNNRTG	8 cut(s)	32, 683, 828, 1232, 1404, 1669, 1728, 2666
Msp20I	TGGCCA	1 cut(s)	773
MspA1I	CMGCKG	6 cut(s)	473, 926, 1865, 2207, 2306, 2344
MspI	CCGG	6 cut(s)	125, 1047, 1618, 2073, 2520, 2959
MspR9I	CCNGG	4 cut(s)	432, 1356, 1619, 1682
MunI	CAATTG	1 cut(s)	1511
Mva1269I	GAATGC	2 cut(s)	982, 1770
MvaI	CCWGG	3 cut(s)	432, 1356, 1682
NciI	CCSGG	1 cut(s)	1619
NcoI	CCATGG	2 cut(s)	1701, 2127
NdeI	CATATG	1 cut(s)	791
NlaIV	GGNNCC	3 cut(s)	57, 267, 1640
NmuCI	GTSAC	6 cut(s)	25, 727, 821, 830, 1037, 2999
NsbI	TGCGCA	1 cut(s)	90
NsiI	ATGCAT	1 cut(s)	1814
NspI	RCATGY	3 cut(s)	98, 1112, 2944
OliI	CACNNNNGTG	2 cut(s)	32, 828
PaeI	GCATGC	1 cut(s)	2944
PaqCI	CACCTGC	1 cut(s)	2104
PceI	AGGCCT	1 cut(s)	2039
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PctI	GAATGC	2 cut(s)	982, 1770
PdmI	GAANNNNTTC	1 cut(s)	918
PfeI	GAWTC	6 cut(s)	701, 1364, 1631, 1868, 2408, 2993
PinAI	ACCGGT	1 cut(s)	2072
PleI	GAGTC	6 cut(s)	71, 574, 1264, 1315, 1722, 2585
PmaCI	CACGTG	1 cut(s)	2999
PmlI	CACGTG	1 cut(s)	2999
PpsI	GAGTC	6 cut(s)	71, 574, 1264, 1315, 1722, 2585
Ppu21I	YACGTR	3 cut(s)	356, 512, 2999
PshBI	ATTAAT	1 cut(s)	234
Psp6I	CCWGG	3 cut(s)	430, 1354, 1680
PspCI	CACGTG	1 cut(s)	2999
PspGI	CCWGG	3 cut(s)	430, 1354, 1680
PspN4I	GGNNCC	3 cut(s)	57, 267, 1640
PspPI	GGNCC	4 cut(s)	55, 67, 1678, 2555
PstI	CTGCAG	2 cut(s)	307, 1037
PsuI	RGATCY	3 cut(s)	189, 1738, 1909
PvuII	CAGCTG	6 cut(s)	473, 926, 1865, 2207, 2306, 2344
RsaI	GTAC	7 cut(s)	107, 625, 763, 798, 1104, 1180, 2310
RsaNI	GTAC	7 cut(s)	106, 624, 762, 797, 1103, 1179, 2309
RseI	CAYNNNNRTG	8 cut(s)	32, 683, 828, 1232, 1404, 1669, 1728, 2666
SaqAI	TTAA	6 cut(s)	234, 251, 1166, 1443, 2085, 2691
Sau96I	GGNCC	4 cut(s)	55, 67, 1678, 2555
ScaI	AGTACT	1 cut(s)	1180
SchI	GAGTC	6 cut(s)	71, 575, 1265, 1316, 1723, 2585
ScrFI	CCNGG	4 cut(s)	432, 1356, 1619, 1682
SduI	GDGCHC	1 cut(s)	1540
SfaNI	GCATC	6 cut(s)	483, 1550, 1661, 1686, 2016, 2080
SfcI	CTRYAG	4 cut(s)	303, 1023, 1033, 1709
SinI	GGWCC	2 cut(s)	55, 2555
SmiMI	CAYNNNNRTG	8 cut(s)	32, 683, 828, 1232, 1404, 1669, 1728, 2666
SmlI	CTYRAG	2 cut(s)	344, 563
SmoI	CTYRAG	2 cut(s)	344, 563
SpeI	ACTAGT	1 cut(s)	1610
SphI	GCATGC	1 cut(s)	2944
SseBI	AGGCCT	1 cut(s)	2039
SsiI	CCGC	9 cut(s)	203, 388, 690, 1693, 1879, 2641, 2816, 2944, 2968
SspI	AATATT	4 cut(s)	610, 817, 2275, 2802
SspMI	CTAG	5 cut(s)	648, 1557, 1611, 2001, 2330
StuI	AGGCCT	1 cut(s)	2039
StyD4I	CCNGG	4 cut(s)	430, 1354, 1617, 1680
StyI	CCWWGG	3 cut(s)	774, 1701, 2127
TaiI	ACGT	5 cut(s)	358, 514, 1408, 2811, 3001
TaqI	TCGA	3 cut(s)	19, 2768, 2901
TatI	WGTACW	4 cut(s)	796, 1102, 1178, 2308
TauI	GCSGC	4 cut(s)	206, 693, 1881, 2971
TfiI	GAWTC	6 cut(s)	701, 1364, 1631, 1868, 2408, 2993
Tru1I	TTAA	6 cut(s)	234, 251, 1166, 1443, 2085, 2691
Tru9I	TTAA	6 cut(s)	234, 251, 1166, 1443, 2085, 2691
TscAI	CASTG	9 cut(s)	580, 685, 1042, 1059, 1138, 1144, 1936, 2389, 2716
TseFI	GTSAC	6 cut(s)	25, 727, 821, 830, 1037, 2999
TseI	GCWGC	9 cut(s)	293, 302, 385, 902, 1017, 1500, 2204, 2303, 2344
Tsp45I	GTSAC	6 cut(s)	25, 727, 821, 830, 1037, 2999
TspGWI	ACGGA	5 cut(s)	13, 731, 1316, 2730, 2824
TspRI	CASTG	9 cut(s)	580, 685, 1042, 1059, 1138, 1144, 1936, 2389, 2716
VpaK11BI	GGWCC	2 cut(s)	55, 2555
VspI	ATTAAT	1 cut(s)	234
XapI	RAATTY	3 cut(s)	465, 755, 2584
XbaI	TCTAGA	1 cut(s)	1556
XceI	RCATGY	3 cut(s)	98, 1112, 2944
XmiI	GTMKAC	1 cut(s)	1708
XmnI	GAANNNNTTC	1 cut(s)	918
XspI	CTAG	5 cut(s)	648, 1557, 1611, 2001, 2330
ZrmI	AGTACT	1 cut(s)	1180
Zsp2I	ATGCAT	1 cut(s)	1814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.