This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 190 |
| Acc36I | ACCTGC | 2 cut(s) | 265, 1402 |
| Acc65I | GGTACC | 1 cut(s) | 181 |
| AccB1I | GGYRCC | 1 cut(s) | 181 |
| AccI | GTMKAC | 1 cut(s) | 5 |
| AciI | CCGC | 4 cut(s) | 16, 43, 488, 1083 |
| AclWI | GGATC | 3 cut(s) | 662, 675, 1359 |
| AcoI | YGGCCR | 2 cut(s) | 105, 1254 |
| AcsI | RAATTY | 1 cut(s) | 762 |
| AcuI | CTGAAG | 1 cut(s) | 846 |
| AfaI | GTAC | 8 cut(s) | 183, 255, 500, 833, 843, 1427, 1448, 1475 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 664 |
| AflII | CTTAAG | 1 cut(s) | 1102 |
| AgsI | TTSAA | 9 cut(s) | 268, 326, 521, 986, 1021, 1057, 1066, 1073, 1278 |
| AjnI | CCWGG | 2 cut(s) | 978, 1251 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 161, 193, 289, 321 |
| AluBI | AGCT | 6 cut(s) | 174, 368, 378, 676, 1141, 1235 |
| AluI | AGCT | 6 cut(s) | 174, 368, 378, 676, 1141, 1235 |
| Alw21I | GWGCWC | 2 cut(s) | 176, 976 |
| Alw26I | GTCTC | 2 cut(s) | 449, 1276 |
| Alw44I | GTGCAC | 1 cut(s) | 972 |
| AlwI | GGATC | 3 cut(s) | 662, 675, 1359 |
| Ama87I | CYCGRG | 1 cut(s) | 781 |
| AoxI | GGCC | 5 cut(s) | 48, 105, 737, 1131, 1254 |
| ApaLI | GTGCAC | 1 cut(s) | 972 |
| ApeKI | GCWGC | 3 cut(s) | 1121, 1235, 1303 |
| ApoI | RAATTY | 1 cut(s) | 762 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 329, 361 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 529 |
| Asp718I | GGTACC | 1 cut(s) | 181 |
| AspLEI | GCGC | 3 cut(s) | 121, 191, 233 |
| AspS9I | GGNCC | 1 cut(s) | 1131 |
| AsuHPI | GGTGA | 2 cut(s) | 220, 739 |
| AsuII | TTCGAA | 1 cut(s) | 167 |
| AsuNHI | GCTAGC | 1 cut(s) | 227 |
| AvaI | CYCGRG | 1 cut(s) | 781 |
| BaeGI | GKGCMC | 1 cut(s) | 976 |
| BalI | TGGCCA | 2 cut(s) | 107, 1256 |
| BamHI | GGATCC | 1 cut(s) | 667 |
| BanI | GGYRCC | 1 cut(s) | 181 |
| BanII | GRGCYC | 2 cut(s) | 99, 176 |
| Bbv12I | GWGCWC | 2 cut(s) | 176, 976 |
| BbvI | GCAGC | 3 cut(s) | 1108, 1222, 1315 |
| BccI | CCATC | 4 cut(s) | 425, 626, 1153, 1365 |
| BceAI | ACGGC | 1 cut(s) | 106 |
| BciT130I | CCWGG | 2 cut(s) | 980, 1253 |
| BcoDI | GTCTC | 2 cut(s) | 449, 1276 |
| BfaI | CTAG | 5 cut(s) | 228, 375, 926, 1196, 1386 |
| BfmI | CTRYAG | 2 cut(s) | 598, 1467 |
| BfoI | RGCGCY | 2 cut(s) | 122, 234 |
| BfrI | CTTAAG | 1 cut(s) | 1102 |
| BfuAI | ACCTGC | 2 cut(s) | 265, 1402 |
| BglII | AGATCT | 1 cut(s) | 966 |
| BisI | GCNGC | 5 cut(s) | 44, 1083, 1122, 1236, 1304 |
| BlpI | GCTNAGC | 1 cut(s) | 93 |
| BlsI | GCNGC | 5 cut(s) | 45, 1084, 1123, 1237, 1305 |
| BmcAI | AGTACT | 1 cut(s) | 1448 |
| Bme1390I | CCNGG | 2 cut(s) | 980, 1253 |
| BmeT110I | CYCGRG | 1 cut(s) | 781 |
| BmgT120I | GGNCC | 1 cut(s) | 1131 |
| BmiI | GGNNCC | 4 cut(s) | 183, 669, 896, 1132 |
| BmrFI | CCNGG | 2 cut(s) | 980, 1253 |
| BmsI | GCATC | 2 cut(s) | 728, 959 |
| BmtI | GCTAGC | 1 cut(s) | 231 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 93 |
| Bpu14I | TTCGAA | 1 cut(s) | 167 |
| BpuEI | CTTGAG | 1 cut(s) | 1110 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1356 |
| BsaI | GGTCTC | 1 cut(s) | 1276 |
| BsaJI | CCNNGG | 3 cut(s) | 204, 978, 1286 |
| BsaWI | WCCGGW | 1 cut(s) | 31 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 426, 456 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 664 |
| Bse1I | ACTGG | 2 cut(s) | 234, 812 |
| Bse3DI | GCAATG | 3 cut(s) | 154, 381, 639 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1356 |
| BseBI | CCWGG | 2 cut(s) | 980, 1253 |
| BseDI | CCNNGG | 3 cut(s) | 204, 978, 1286 |
| BseGI | GGATG | 3 cut(s) | 415, 436, 457 |
| BseJI | GATNNNNATC | 1 cut(s) | 1356 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 664 |
| BseMI | GCAATG | 3 cut(s) | 154, 381, 639 |
| BseMII | CTCAG | 2 cut(s) | 84, 714 |
| BseNI | ACTGG | 2 cut(s) | 234, 812 |
| BseRI | GAGGAG | 2 cut(s) | 360, 977 |
| BseSI | GKGCMC | 1 cut(s) | 976 |
| BseXI | GCAGC | 3 cut(s) | 1108, 1222, 1315 |
| BsgI | GTGCAG | 1 cut(s) | 303 |
| BshFI | GGCC | 5 cut(s) | 50, 107, 739, 1133, 1256 |
| BshNI | GGYRCC | 1 cut(s) | 181 |
| BsiHKAI | GWGCWC | 2 cut(s) | 176, 976 |
| BsiHKCI | CYCGRG | 1 cut(s) | 781 |
| BsiSI | CCGG | 1 cut(s) | 32 |
| BslFI | GGGAC | 1 cut(s) | 380 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 664 |
| BsmAI | GTCTC | 2 cut(s) | 449, 1276 |
| BsmFI | GGGAC | 1 cut(s) | 380 |
| BsmI | GAATGC | 2 cut(s) | 1297, 1346 |
| BsnI | GGCC | 5 cut(s) | 50, 107, 739, 1133, 1256 |
| Bso31I | GGTCTC | 1 cut(s) | 1276 |
| BsoBI | CYCGRG | 1 cut(s) | 781 |
| Bsp119I | TTCGAA | 1 cut(s) | 167 |
| Bsp1286I | GDGCHC | 3 cut(s) | 99, 176, 976 |
| Bsp143I | GATC | 5 cut(s) | 20, 538, 667, 966, 1351 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 93 |
| Bsp19I | CCATGG | 1 cut(s) | 1286 |
| BspACI | CCGC | 4 cut(s) | 16, 43, 488, 1083 |
| BspANI | GGCC | 5 cut(s) | 50, 107, 739, 1133, 1256 |
| BspCNI | CTCAG | 2 cut(s) | 85, 715 |
| BspLI | GGNNCC | 4 cut(s) | 183, 669, 896, 1132 |
| BspMAI | CTGCAG | 1 cut(s) | 602 |
| BspMI | ACCTGC | 2 cut(s) | 265, 1402 |
| BspOI | GCTAGC | 1 cut(s) | 231 |
| BspPI | GGATC | 3 cut(s) | 662, 675, 1359 |
| BspQI | GCTCTTC | 1 cut(s) | 164 |
| BspT104I | TTCGAA | 1 cut(s) | 167 |
| BspT107I | GGYRCC | 1 cut(s) | 181 |
| BspTI | CTTAAG | 1 cut(s) | 1102 |
| BspTNI | GGTCTC | 1 cut(s) | 1276 |
| BsrDI | GCAATG | 3 cut(s) | 154, 381, 639 |
| BsrI | ACTGG | 2 cut(s) | 234, 812 |
| BssECI | CCNNGG | 3 cut(s) | 204, 978, 1286 |
| BssMI | GATC | 5 cut(s) | 20, 538, 667, 966, 1351 |
| BssT1I | CCWWGG | 2 cut(s) | 204, 1286 |
| Bst2UI | CCWGG | 2 cut(s) | 980, 1253 |
| Bst4CI | ACNGT | 5 cut(s) | 659, 865, 915, 921, 1415 |
| Bst6I | CTCTTC | 5 cut(s) | 164, 822, 981, 1020, 1197 |
| BstAFI | CTTAAG | 1 cut(s) | 1102 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 689 |
| BstBI | TTCGAA | 1 cut(s) | 167 |
| BstC8I | GCNNGC | 7 cut(s) | 48, 117, 229, 717, 788, 792, 1208 |
| BstDEI | CTNAG | 2 cut(s) | 93, 723 |
| BstDSI | CCRYGG | 1 cut(s) | 1286 |
| BstF5I | GGATG | 3 cut(s) | 415, 436, 457 |
| BstH2I | RGCGCY | 2 cut(s) | 122, 234 |
| BstHHI | GCGC | 3 cut(s) | 121, 191, 233 |
| BstKTI | GATC | 5 cut(s) | 23, 541, 670, 969, 1354 |
| BstMAI | GTCTC | 2 cut(s) | 449, 1276 |
| BstMBI | GATC | 5 cut(s) | 20, 538, 667, 966, 1351 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 689, 1130, 1241 |
| BstNI | CCWGG | 2 cut(s) | 980, 1253 |
| BstNSI | RCATGY | 2 cut(s) | 790, 1210 |
| BstSCI | CCNGG | 2 cut(s) | 978, 1251 |
| BstSFI | CTRYAG | 2 cut(s) | 598, 1467 |
| BstSLI | GKGCMC | 1 cut(s) | 976 |
| BstV1I | GCAGC | 3 cut(s) | 1108, 1222, 1315 |
| BstX2I | RGATCY | 3 cut(s) | 667, 966, 1351 |
| BstYI | RGATCY | 3 cut(s) | 667, 966, 1351 |
| BsuRI | GGCC | 5 cut(s) | 50, 107, 739, 1133, 1256 |
| BtgI | CCRYGG | 1 cut(s) | 1286 |
| BtgZI | GCGATG | 1 cut(s) | 1343 |
| BtsCI | GGATG | 3 cut(s) | 415, 436, 457 |
| BveI | ACCTGC | 2 cut(s) | 265, 1402 |
| Cac8I | GCNNGC | 7 cut(s) | 48, 117, 229, 717, 788, 792, 1208 |
| CfoI | GCGC | 3 cut(s) | 121, 191, 233 |
| Cfr13I | GGNCC | 1 cut(s) | 1131 |
| CseI | GACGC | 1 cut(s) | 1333 |
| Csp6I | GTAC | 8 cut(s) | 182, 254, 499, 832, 842, 1426, 1447, 1474 |
| CviAII | CATG | 8 cut(s) | 186, 406, 575, 664, 754, 787, 1207, 1287 |
| CviQI | GTAC | 8 cut(s) | 182, 254, 499, 832, 842, 1426, 1447, 1474 |
| DdeI | CTNAG | 2 cut(s) | 93, 723 |
| DpnI | GATC | 5 cut(s) | 22, 540, 669, 968, 1353 |
| DpnII | GATC | 5 cut(s) | 20, 538, 667, 966, 1351 |
| EaeI | YGGCCR | 2 cut(s) | 105, 1254 |
| Eam1104I | CTCTTC | 5 cut(s) | 164, 822, 981, 1020, 1197 |
| EarI | CTCTTC | 5 cut(s) | 164, 822, 981, 1020, 1197 |
| Ecl136II | GAGCTC | 1 cut(s) | 174 |
| Eco130I | CCWWGG | 2 cut(s) | 204, 1286 |
| Eco147I | AGGCCT | 2 cut(s) | 50, 739 |
| Eco24I | GRGCYC | 2 cut(s) | 99, 176 |
| Eco31I | GGTCTC | 1 cut(s) | 1276 |
| Eco32I | GATATC | 2 cut(s) | 700, 884 |
| Eco53kI | GAGCTC | 1 cut(s) | 174 |
| Eco57I | CTGAAG | 1 cut(s) | 846 |
| Eco88I | CYCGRG | 1 cut(s) | 781 |
| EcoICRI | GAGCTC | 1 cut(s) | 174 |
| EcoRII | CCWGG | 2 cut(s) | 978, 1251 |
| EcoRV | GATATC | 2 cut(s) | 700, 884 |
| EcoT14I | CCWWGG | 2 cut(s) | 204, 1286 |
| EcoT38I | GRGCYC | 2 cut(s) | 99, 176 |
| ErhI | CCWWGG | 2 cut(s) | 204, 1286 |
| FaeI | CATG | 8 cut(s) | 189, 409, 578, 667, 757, 790, 1210, 1290 |
| FaqI | GGGAC | 1 cut(s) | 380 |
| FatI | CATG | 8 cut(s) | 185, 405, 574, 663, 753, 786, 1206, 1286 |
| FblI | GTMKAC | 1 cut(s) | 5 |
| Fnu4HI | GCNGC | 5 cut(s) | 44, 1083, 1122, 1236, 1304 |
| FokI | GGATG | 3 cut(s) | 422, 443, 464 |
| FriOI | GRGCYC | 2 cut(s) | 99, 176 |
| Fsp4HI | GCNGC | 5 cut(s) | 44, 1083, 1122, 1236, 1304 |
| FspBI | CTAG | 5 cut(s) | 228, 375, 926, 1196, 1386 |
| FspI | TGCGCA | 1 cut(s) | 190 |
| GlaI | GCGC | 3 cut(s) | 120, 190, 232 |
| GluI | GCNGC | 5 cut(s) | 44, 1083, 1122, 1236, 1304 |
| HaeII | RGCGCY | 2 cut(s) | 122, 234 |
| HaeIII | GGCC | 5 cut(s) | 50, 107, 739, 1133, 1256 |
| HapII | CCGG | 1 cut(s) | 32 |
| HgaI | GACGC | 1 cut(s) | 1333 |
| HhaI | GCGC | 3 cut(s) | 121, 191, 233 |
| Hin1II | CATG | 8 cut(s) | 189, 409, 578, 667, 757, 790, 1210, 1290 |
| Hin6I | GCGC | 3 cut(s) | 119, 189, 231 |
| HinP1I | GCGC | 3 cut(s) | 119, 189, 231 |
| HincII | GTYRAC | 3 cut(s) | 6, 465, 805 |
| HindII | GTYRAC | 3 cut(s) | 6, 465, 805 |
| HinfI | GANTC | 7 cut(s) | 139, 413, 437, 473, 484, 530, 1043 |
| HpaII | CCGG | 1 cut(s) | 32 |
| HphI | GGTGA | 2 cut(s) | 220, 739 |
| Hpy166II | GTNNAC | 7 cut(s) | 6, 342, 465, 805, 868, 974, 1391 |
| Hpy188I | TCNGA | 8 cut(s) | 135, 144, 430, 571, 724, 768, 1330, 1403 |
| Hpy188III | TCNNGA | 2 cut(s) | 76, 1196 |
| Hpy8I | GTNNAC | 7 cut(s) | 6, 342, 465, 805, 868, 974, 1391 |
| Hpy99I | CGWCG | 5 cut(s) | 58, 61, 64, 67, 70 |
| HpyAV | CCTTC | 5 cut(s) | 620, 750, 763, 1284, 1335 |
| HpyCH4III | ACNGT | 5 cut(s) | 659, 865, 915, 921, 1415 |
| HpyCH4IV | ACGT | 1 cut(s) | 344 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 689, 1130, 1241 |
| HpyF3I | CTNAG | 2 cut(s) | 93, 723 |
| HpySE526I | ACGT | 1 cut(s) | 344 |
| Hsp92II | CATG | 8 cut(s) | 189, 409, 578, 667, 757, 790, 1210, 1290 |
| HspAI | GCGC | 3 cut(s) | 119, 189, 231 |
| KpnI | GGTACC | 1 cut(s) | 185 |
| Kzo9I | GATC | 5 cut(s) | 20, 538, 667, 966, 1351 |
| LguI | GCTCTTC | 1 cut(s) | 164 |
| LmnI | GCTCC | 4 cut(s) | 144, 373, 383, 1225 |
| Lsp1109I | GCAGC | 3 cut(s) | 1108, 1222, 1315 |
| LweI | GCATC | 2 cut(s) | 728, 959 |
| MaeI | CTAG | 5 cut(s) | 228, 375, 926, 1196, 1386 |
| MaeII | ACGT | 1 cut(s) | 344 |
| MaeIII | GTNAC | 7 cut(s) | 236, 301, 401, 915, 921, 995, 1224 |
| MalI | GATC | 5 cut(s) | 22, 540, 669, 968, 1353 |
| MboI | GATC | 5 cut(s) | 20, 538, 667, 966, 1351 |
| MboII | GAAGA | 9 cut(s) | 181, 374, 742, 839, 998, 1037, 1038, 1184, 1240 |
| MflI | RGATCY | 3 cut(s) | 667, 966, 1351 |
| MhlI | GDGCHC | 3 cut(s) | 99, 176, 976 |
| MlsI | TGGCCA | 2 cut(s) | 107, 1256 |
| MluCI | AATT | 6 cut(s) | 525, 762, 1057, 1258, 1381, 1461 |
| MluNI | TGGCCA | 2 cut(s) | 107, 1256 |
| MlyI | GAGTC | 1 cut(s) | 493 |
| MmeI | TCCRAC | 2 cut(s) | 603, 1137 |
| MnlI | CCTC | 8 cut(s) | 61, 236, 381, 569, 718, 811, 955, 1021 |
| Mox20I | TGGCCA | 2 cut(s) | 107, 1256 |
| MroXI | GAANNNNTTC | 1 cut(s) | 529 |
| MscI | TGGCCA | 2 cut(s) | 107, 1256 |
| MseI | TTAA | 4 cut(s) | 273, 480, 777, 1103 |
| MslI | CAYNNNNRTG | 1 cut(s) | 714 |
| Msp20I | TGGCCA | 2 cut(s) | 107, 1256 |
| MspCI | CTTAAG | 1 cut(s) | 1102 |
| MspI | CCGG | 1 cut(s) | 32 |
| MspR9I | CCNGG | 2 cut(s) | 980, 1253 |
| Mva1269I | GAATGC | 2 cut(s) | 1297, 1346 |
| MvaI | CCWGG | 2 cut(s) | 980, 1253 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 689, 1130, 1241 |
| NcoI | CCATGG | 1 cut(s) | 1286 |
| NdeII | GATC | 5 cut(s) | 20, 538, 667, 966, 1351 |
| NheI | GCTAGC | 1 cut(s) | 227 |
| NlaIII | CATG | 8 cut(s) | 189, 409, 578, 667, 757, 790, 1210, 1290 |
| NlaIV | GGNNCC | 4 cut(s) | 183, 669, 896, 1132 |
| NmuCI | GTSAC | 1 cut(s) | 1224 |
| NsbI | TGCGCA | 1 cut(s) | 190 |
| NspI | RCATGY | 2 cut(s) | 790, 1210 |
| NspV | TTCGAA | 1 cut(s) | 167 |
| PaeI | GCATGC | 2 cut(s) | 790, 1210 |
| PceI | AGGCCT | 2 cut(s) | 50, 739 |
| PciSI | GCTCTTC | 1 cut(s) | 164 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 59, 62, 65, 173 |
| PctI | GAATGC | 2 cut(s) | 1297, 1346 |
| PdmI | GAANNNNTTC | 1 cut(s) | 529 |
| PfeI | GAWTC | 6 cut(s) | 139, 413, 437, 473, 530, 1043 |
| PflFI | GACNNNGTC | 1 cut(s) | 460 |
| PkrI | GCNGC | 5 cut(s) | 45, 1084, 1123, 1237, 1305 |
| PleI | GAGTC | 1 cut(s) | 492 |
| PpsI | GAGTC | 1 cut(s) | 492 |
| Psp124BI | GAGCTC | 1 cut(s) | 176 |
| Psp6I | CCWGG | 2 cut(s) | 978, 1251 |
| PspGI | CCWGG | 2 cut(s) | 978, 1251 |
| PspN4I | GGNNCC | 4 cut(s) | 183, 669, 896, 1132 |
| PspPI | GGNCC | 1 cut(s) | 1131 |
| PstI | CTGCAG | 1 cut(s) | 602 |
| PsuI | RGATCY | 3 cut(s) | 667, 966, 1351 |
| PsyI | GACNNNGTC | 1 cut(s) | 460 |
| RsaI | GTAC | 8 cut(s) | 183, 255, 500, 833, 843, 1427, 1448, 1475 |
| RsaNI | GTAC | 8 cut(s) | 182, 254, 499, 832, 842, 1426, 1447, 1474 |
| RseI | CAYNNNNRTG | 1 cut(s) | 714 |
| SacI | GAGCTC | 1 cut(s) | 176 |
| SalI | GTCGAC | 1 cut(s) | 4 |
| SapI | GCTCTTC | 1 cut(s) | 164 |
| SaqAI | TTAA | 4 cut(s) | 273, 480, 777, 1103 |
| SatI | GCNGC | 5 cut(s) | 44, 1083, 1122, 1236, 1304 |
| Sau3AI | GATC | 5 cut(s) | 20, 538, 667, 966, 1351 |
| Sau96I | GGNCC | 1 cut(s) | 1131 |
| ScaI | AGTACT | 1 cut(s) | 1448 |
| SchI | GAGTC | 1 cut(s) | 493 |
| ScrFI | CCNGG | 2 cut(s) | 980, 1253 |
| SduI | GDGCHC | 3 cut(s) | 99, 176, 976 |
| SfaNI | GCATC | 2 cut(s) | 728, 959 |
| SfcI | CTRYAG | 2 cut(s) | 598, 1467 |
| SfuI | TTCGAA | 1 cut(s) | 167 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 714 |
| SmlI | CTYRAG | 2 cut(s) | 1102, 1125 |
| SmoI | CTYRAG | 2 cut(s) | 1102, 1125 |
| SphI | GCATGC | 2 cut(s) | 790, 1210 |
| Sse9I | AATT | 6 cut(s) | 525, 762, 1057, 1258, 1381, 1461 |
| SseBI | AGGCCT | 2 cut(s) | 50, 739 |
| SsiI | CCGC | 4 cut(s) | 16, 43, 488, 1083 |
| SspMI | CTAG | 5 cut(s) | 228, 375, 926, 1196, 1386 |
| SstI | GAGCTC | 1 cut(s) | 176 |
| StuI | AGGCCT | 2 cut(s) | 50, 739 |
| StyD4I | CCNGG | 2 cut(s) | 978, 1251 |
| StyI | CCWWGG | 2 cut(s) | 204, 1286 |
| TaaI | ACNGT | 5 cut(s) | 659, 865, 915, 921, 1415 |
| TaiI | ACGT | 1 cut(s) | 347 |
| TaqI | TCGA | 4 cut(s) | 5, 68, 167, 541 |
| TaqII | GACCGA | 1 cut(s) | 894 |
| TasI | AATT | 6 cut(s) | 525, 762, 1057, 1258, 1381, 1461 |
| TatI | WGTACW | 4 cut(s) | 498, 831, 1425, 1446 |
| TauI | GCSGC | 2 cut(s) | 46, 1085 |
| TfiI | GAWTC | 6 cut(s) | 139, 413, 437, 473, 530, 1043 |
| Tru1I | TTAA | 4 cut(s) | 273, 480, 777, 1103 |
| Tru9I | TTAA | 4 cut(s) | 273, 480, 777, 1103 |
| TseFI | GTSAC | 1 cut(s) | 1224 |
| TseI | GCWGC | 3 cut(s) | 1121, 1235, 1303 |
| Tsp45I | GTSAC | 1 cut(s) | 1224 |
| TspDTI | ATGAA | 3 cut(s) | 375, 652, 917 |
| TspGWI | ACGGA | 1 cut(s) | 1212 |
| Tth111I | GACNNNGTC | 1 cut(s) | 460 |
| Vha464I | CTTAAG | 1 cut(s) | 1102 |
| VneI | GTGCAC | 1 cut(s) | 972 |
| XapI | RAATTY | 1 cut(s) | 762 |
| XbaI | TCTAGA | 1 cut(s) | 1195 |
| XceI | RCATGY | 2 cut(s) | 790, 1210 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 751 |
| XmiI | GTMKAC | 1 cut(s) | 5 |
| XmnI | GAANNNNTTC | 1 cut(s) | 529 |
| XspI | CTAG | 5 cut(s) | 228, 375, 926, 1196, 1386 |
| ZrmI | AGTACT | 1 cut(s) | 1448 |