Rorug03G0274400

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001851
Physical Location & Seq
Reverse (-)
27113194 .. 27120553
7360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0274400.1

Sequence Viewer

Length: 1158 bp
ATGATGATGATGATGATGATGGGAAGAAGGGTGAGGGTGTTGTGCTGCTTCACCACCTTTACATATGTGTATCTCTACTCCCTTGTCGTGGCGGCTTTGCCAACCATACCGGACGGTGATCACCATCTGCTACTGCCCGGTGCTGGCGAGTCATTCAAGATCGCTCTCTTTGCCGACCTGCATTTCGGAGAGGCCGCCTCCACCGACTGGGGTCCACTCCAAGATGTCAACTCCCTCAGAGTCATGTCCACTGTGCTTGATCATGAAATCCCAGATTTTGTTATCTATCTTGGAGATGTCATCACAGCCAACAATATAGCAATTAAAAATGCAAGCTTGTATTGGGATCAGGCAATTTCTCCAACAAGAGCCAGAGGAATTCCTTGGGCCAGTGTTTTTGGAAACCATGATGATGCGGCATTTGAGTGGCCGATGGAGTGGTTTTCAGGTCCCGGAATTCCTGAAATTCGTTGCTCTGTAGCCAATTCCTCATGCTCAGGAGAAGAAGACTGTAGCTTCAAAGGCACACATCGTCTGGAGTTGATGAAAAATGAGATTGCACTTGGCCCTCTTTCATATTCTCGATATGGTCCTAAAGAACTTTGGCCTAGTGTATCTAACTATGTCCTCCAAGTTTCTTCGTCAAACAATCCCGGGTCACCTGTGGCATTTTTATATTTCCTAGATTCTGGTGGTGGTTCCTACCCAGAAGTTTTATCAAGTGCCCAAGCAGAATGGTTCCGTCAGAAAGCTCTAGAGATCAACCCTGATTCAAGGGTGCCTGAGCTAATCTTTTGGCATATACCAAGTCGTGCTTACAAGAAAGCAGCTCCAAGATTTGGTATACACAAGCCTTGTGTCGGTTCAATTAATAAGGAGAGAGTAGCAACTCAAGACGCTGAAAATGGCATCATGAAACTTTTGGCTGAAAGGGTTTCTGTCAAGGCAATATTTGTAGGACACAACCATGGATTGGACTGGTGCTGCCCTTATAAGAAACTGTGGCTATGCTTTGCTCGGCATACTGGTTATGGTGGCTATGGAAACTGGGCTAGAGGAGCTAGAATCATAGAAATCACCCAGCAGCCTTTCTCTATTAAATCTTGGATTAGAATGGAGGATGGTAGTGTACATAGTGAACTTGTCTTGAGTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

385

Amino Acids

42.95

Weight (kDa)

6.31

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 52 - 323 2.4e-10 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
Acc36I ACCTGC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 778
AccB7I CCANNNNNTGG 3 cut(s) 207, 839, 973
AccI GTMKAC 1 cut(s) 844
AciI CCGC 3 cut(s) 92, 195, 416
AclWI GGATC 1 cut(s) 354
AcoI YGGCCR 1 cut(s) 428
AcsI RAATTY 3 cut(s) 378, 456, 465
AfaI GTAC 1 cut(s) 1131
AfiI CCNNNNNNNGG 6 cut(s) 88, 143, 207, 839, 860, 973
AgsI TTSAA 4 cut(s) 157, 520, 774, 867
AluBI AGCT 6 cut(s) 336, 516, 752, 787, 830, 1061
AluI AGCT 6 cut(s) 336, 516, 752, 787, 830, 1061
AlwI GGATC 1 cut(s) 354
Ama87I CYCGRG 1 cut(s) 653
AoxI GGCC 5 cut(s) 192, 387, 428, 565, 605
ApeKI GCWGC 4 cut(s) 45, 827, 984, 1084
ApoI RAATTY 3 cut(s) 378, 456, 465
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AseI ATTAAT 1 cut(s) 870
AspS9I GGNCC 5 cut(s) 212, 387, 449, 566, 590
AsuC2I CCSGG 4 cut(s) 138, 453, 654, 655
AsuHPI GGTGA 6 cut(s) 43, 43, 113, 128, 651, 1069
AvaI CYCGRG 1 cut(s) 653
AvaII GGWCC 3 cut(s) 212, 449, 590
BaeGI GKGCMC 1 cut(s) 727
BanI GGYRCC 1 cut(s) 778
BbsI GAAGAC 1 cut(s) 513
BbvI GCAGC 4 cut(s) 32, 839, 971, 1096
BccI CCATC 4 cut(s) 13, 132, 427, 1115
BclI TGATCA 2 cut(s) 118, 259
BcnI CCSGG 4 cut(s) 138, 453, 654, 655
BfaI CTAG 5 cut(s) 609, 683, 755, 1053, 1062
BfmI CTRYAG 2 cut(s) 477, 511
BfuAI ACCTGC 1 cut(s) 186
BisI GCNGC 7 cut(s) 46, 93, 195, 417, 828, 985, 1085
BlsI GCNGC 7 cut(s) 47, 94, 196, 418, 829, 986, 1086
Bme1390I CCNGG 4 cut(s) 138, 453, 654, 655
Bme18I GGWCC 3 cut(s) 212, 449, 590
BmeT110I CYCGRG 1 cut(s) 653
BmgT120I GGNCC 5 cut(s) 212, 387, 449, 566, 590
BmiI GGNNCC 5 cut(s) 213, 451, 700, 740, 780
BmrFI CCNGG 4 cut(s) 138, 453, 654, 655
BmrI ACTGGG 2 cut(s) 217, 1057
BmsI GCATC 2 cut(s) 403, 918
BmuI ACTGGG 2 cut(s) 217, 1057
BoxI GACNNNNGTC 1 cut(s) 210
BpiI GAAGAC 1 cut(s) 513
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 1 cut(s) 557
Bpu10I CCTNAGC 2 cut(s) 496, 783
BpuEI CTTGAG 1 cut(s) 876
BpuMI CCSGG 4 cut(s) 138, 453, 654, 655
BsaBI GATNNNNATC 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 383, 653, 967
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 143, 207, 839, 860, 973
Bse1I ACTGG 5 cut(s) 212, 390, 983, 1030, 1052
Bse8I GATNNNNATC 1 cut(s) 123
BseDI CCNNGG 3 cut(s) 383, 653, 967
BseGI GGATG 1 cut(s) 1126
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 6 cut(s) 88, 143, 207, 839, 860, 973
BseMII CTCAG 3 cut(s) 250, 510, 774
BseNI ACTGG 5 cut(s) 212, 390, 983, 1030, 1052
BseRI GAGGAG 1 cut(s) 1071
BseSI GKGCMC 1 cut(s) 727
BseXI GCAGC 4 cut(s) 32, 839, 971, 1096
BseYI CCCAGC 1 cut(s) 1080
BshFI GGCC 5 cut(s) 194, 389, 430, 567, 607
BshNI GGYRCC 1 cut(s) 778
BsiHKCI CYCGRG 1 cut(s) 653
BsiSI CCGG 4 cut(s) 110, 138, 453, 654
BslFI GGGAC 2 cut(s) 435, 1137
BslI CCNNNNNNNGG 6 cut(s) 88, 143, 207, 839, 860, 973
BsmFI GGGAC 2 cut(s) 435, 1137
BsnI GGCC 5 cut(s) 194, 389, 430, 567, 607
BsoBI CYCGRG 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 727
Bsp1407I TGTACA 1 cut(s) 1129
Bsp143I GATC 5 cut(s) 118, 159, 259, 346, 759
Bsp19I CCATGG 1 cut(s) 967
BspACI CCGC 3 cut(s) 92, 195, 416
BspANI GGCC 5 cut(s) 194, 389, 430, 567, 607
BspCNI CTCAG 3 cut(s) 249, 509, 775
BspHI TCATGA 2 cut(s) 262, 912
BspLI GGNNCC 5 cut(s) 213, 451, 700, 740, 780
BspMI ACCTGC 1 cut(s) 186
BspPI GGATC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 778
BsrGI TGTACA 1 cut(s) 1129
BsrI ACTGG 5 cut(s) 212, 390, 983, 1030, 1052
BssECI CCNNGG 3 cut(s) 383, 653, 967
BssMI GATC 5 cut(s) 118, 159, 259, 346, 759
BssNAI GTATAC 1 cut(s) 845
BssT1I CCWWGG 2 cut(s) 383, 967
Bst1107I GTATAC 1 cut(s) 845
Bst4CI ACNGT 4 cut(s) 116, 253, 512, 1002
BstAUI TGTACA 1 cut(s) 1129
BstC8I GCNNGC 2 cut(s) 145, 334
BstDEI CTNAG 3 cut(s) 236, 496, 783
BstDSI CCRYGG 1 cut(s) 967
BstEII GGTNACC 1 cut(s) 657
BstF5I GGATG 1 cut(s) 1126
BstKTI GATC 5 cut(s) 121, 162, 262, 349, 762
BstMBI GATC 5 cut(s) 118, 159, 259, 346, 759
BstMWI GCNNNNNNNGC 3 cut(s) 170, 522, 1058
BstPAI GACNNNNGTC 1 cut(s) 210
BstPI GGTNACC 1 cut(s) 657
BstSCI CCNGG 4 cut(s) 136, 451, 652, 653
BstSFI CTRYAG 2 cut(s) 477, 511
BstSLI GKGCMC 1 cut(s) 727
BstV1I GCAGC 4 cut(s) 32, 839, 971, 1096
BstV2I GAAGAC 1 cut(s) 513
BstZ17I GTATAC 1 cut(s) 845
BsuRI GGCC 5 cut(s) 194, 389, 430, 567, 607
BtgI CCRYGG 1 cut(s) 967
BtsCI GGATG 1 cut(s) 1126
BtsIMutI CAGTG 2 cut(s) 249, 397
BveI ACCTGC 1 cut(s) 186
Cac8I GCNNGC 2 cut(s) 145, 334
CciI TCATGA 2 cut(s) 262, 912
Cfr13I GGNCC 5 cut(s) 212, 387, 449, 566, 590
Cfr9I CCCGGG 1 cut(s) 653
CseI GACGC 1 cut(s) 905
Csp6I GTAC 1 cut(s) 1130
CspCI CAANNNNNGTGG 2 cut(s) 238, 273
CviAII CATG 6 cut(s) 244, 263, 407, 492, 913, 968
CviQI GTAC 1 cut(s) 1130
DdeI CTNAG 3 cut(s) 236, 496, 783
DpnI GATC 5 cut(s) 120, 161, 261, 348, 761
DpnII GATC 5 cut(s) 118, 159, 259, 346, 759
EaeI YGGCCR 1 cut(s) 428
Eco130I CCWWGG 2 cut(s) 383, 967
Eco47I GGWCC 3 cut(s) 212, 449, 590
Eco88I CYCGRG 1 cut(s) 653
Eco91I GGTNACC 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 449
EcoO65I GGTNACC 1 cut(s) 657
EcoRI GAATTC 2 cut(s) 378, 456
EcoT14I CCWWGG 2 cut(s) 383, 967
ErhI CCWWGG 2 cut(s) 383, 967
FaeI CATG 6 cut(s) 247, 266, 410, 495, 916, 971
FalI AAGNNNNNCTT 2 cut(s) 799, 831
FaqI GGGAC 2 cut(s) 435, 1137
FatI CATG 6 cut(s) 243, 262, 406, 491, 912, 967
FauNDI CATATG 1 cut(s) 64
FbaI TGATCA 2 cut(s) 118, 259
FblI GTMKAC 1 cut(s) 844
Fnu4HI GCNGC 7 cut(s) 46, 93, 195, 417, 828, 985, 1085
FokI GGATG 1 cut(s) 1133
Fsp4HI GCNGC 7 cut(s) 46, 93, 195, 417, 828, 985, 1085
FspBI CTAG 5 cut(s) 609, 683, 755, 1053, 1062
GluI GCNGC 7 cut(s) 46, 93, 195, 417, 828, 985, 1085
GsaI CCCAGC 1 cut(s) 1084
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 5 cut(s) 194, 389, 430, 567, 607
HapII CCGG 4 cut(s) 110, 138, 453, 654
HgaI GACGC 1 cut(s) 905
Hin1II CATG 6 cut(s) 247, 266, 410, 495, 916, 971
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 6 cut(s) 149, 240, 686, 770, 1065, 1150
HpaII CCGG 4 cut(s) 110, 138, 453, 654
HphI GGTGA 6 cut(s) 43, 43, 113, 128, 651, 1069
Hpy166II GTNNAC 6 cut(s) 215, 229, 249, 845, 1130, 1139
Hpy188I TCNGA 3 cut(s) 188, 239, 747
Hpy8I GTNNAC 6 cut(s) 215, 229, 249, 845, 1130, 1139
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 4 cut(s) 116, 253, 512, 1002
HpyCH4V TGCA 3 cut(s) 181, 332, 560
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 522, 1058
HpyF3I CTNAG 3 cut(s) 236, 496, 783
Hsp92II CATG 6 cut(s) 247, 266, 410, 495, 916, 971
Ksp22I TGATCA 2 cut(s) 118, 259
Kzo9I GATC 5 cut(s) 118, 159, 259, 346, 759
LmnI GCTCC 2 cut(s) 835, 1058
Lsp1109I GCAGC 4 cut(s) 32, 839, 971, 1096
LweI GCATC 2 cut(s) 403, 918
MaeI CTAG 5 cut(s) 609, 683, 755, 1053, 1062
MaeIII GTNAC 1 cut(s) 657
MalI GATC 5 cut(s) 120, 161, 261, 348, 761
MboI GATC 5 cut(s) 118, 159, 259, 346, 759
MboII GAAGA 4 cut(s) 36, 515, 518, 630
MhlI GDGCHC 1 cut(s) 727
MluCI AATT 7 cut(s) 321, 354, 378, 456, 465, 484, 867
MlyI GAGTC 2 cut(s) 158, 249
MmeI TCCRAC 1 cut(s) 386
MseI TTAA 3 cut(s) 324, 870, 1098
MslI CAYNNNNRTG 3 cut(s) 411, 424, 966
MspI CCGG 4 cut(s) 110, 138, 453, 654
MspR9I CCNGG 4 cut(s) 138, 453, 654, 655
MwoI GCNNNNNNNGC 3 cut(s) 170, 522, 1058
NciI CCSGG 4 cut(s) 138, 453, 654, 655
NcoI CCATGG 1 cut(s) 967
NdeI CATATG 1 cut(s) 64
NdeII GATC 5 cut(s) 118, 159, 259, 346, 759
NlaIII CATG 6 cut(s) 247, 266, 410, 495, 916, 971
NlaIV GGNNCC 5 cut(s) 213, 451, 700, 740, 780
NmeAIII GCCGAG 1 cut(s) 997
NmuCI GTSAC 1 cut(s) 657
PagI TCATGA 2 cut(s) 262, 912
PfeI GAWTC 3 cut(s) 686, 770, 1065
PflMI CCANNNNNTGG 3 cut(s) 207, 839, 973
PfoI TCCNGGA 1 cut(s) 451
PkrI GCNGC 7 cut(s) 47, 94, 196, 418, 829, 986, 1086
PleI GAGTC 2 cut(s) 157, 248
PpsI GAGTC 2 cut(s) 157, 248
PpuMI RGGWCCY 1 cut(s) 449
PshAI GACNNNNGTC 1 cut(s) 210
PshBI ATTAAT 1 cut(s) 870
PsiI TTATAA 1 cut(s) 993
Psp5II RGGWCCY 1 cut(s) 449
PspEI GGTNACC 1 cut(s) 657
PspFI CCCAGC 1 cut(s) 1080
PspN4I GGNNCC 5 cut(s) 213, 451, 700, 740, 780
PspPI GGNCC 5 cut(s) 212, 387, 449, 566, 590
PspPPI RGGWCCY 1 cut(s) 449
RsaI GTAC 1 cut(s) 1131
RsaNI GTAC 1 cut(s) 1130
RseI CAYNNNNRTG 3 cut(s) 411, 424, 966
SaqAI TTAA 3 cut(s) 324, 870, 1098
SatI GCNGC 7 cut(s) 46, 93, 195, 417, 828, 985, 1085
Sau3AI GATC 5 cut(s) 118, 159, 259, 346, 759
Sau96I GGNCC 5 cut(s) 212, 387, 449, 566, 590
SchI GAGTC 2 cut(s) 158, 249
ScrFI CCNGG 4 cut(s) 138, 453, 654, 655
SduI GDGCHC 1 cut(s) 727
SfaNI GCATC 2 cut(s) 403, 918
SfcI CTRYAG 2 cut(s) 477, 511
SinI GGWCC 3 cut(s) 212, 449, 590
SmaI CCCGGG 1 cut(s) 655
SmiMI CAYNNNNRTG 3 cut(s) 411, 424, 966
SmlI CTYRAG 2 cut(s) 891, 1147
SmoI CTYRAG 2 cut(s) 891, 1147
Sse9I AATT 7 cut(s) 321, 354, 378, 456, 465, 484, 867
SsiI CCGC 3 cut(s) 92, 195, 416
SspI AATATT 1 cut(s) 951
SspMI CTAG 5 cut(s) 609, 683, 755, 1053, 1062
StyD4I CCNGG 4 cut(s) 136, 451, 652, 653
StyI CCWWGG 2 cut(s) 383, 967
TaaI ACNGT 4 cut(s) 116, 253, 512, 1002
TaqI TCGA 1 cut(s) 583
TasI AATT 7 cut(s) 321, 354, 378, 456, 465, 484, 867
TatI WGTACW 1 cut(s) 1129
TauI GCSGC 3 cut(s) 95, 197, 419
TfiI GAWTC 3 cut(s) 686, 770, 1065
Tru1I TTAA 3 cut(s) 324, 870, 1098
Tru9I TTAA 3 cut(s) 324, 870, 1098
TscAI CASTG 2 cut(s) 256, 397
TseFI GTSAC 1 cut(s) 657
TseI GCWGC 4 cut(s) 45, 827, 984, 1084
Tsp45I GTSAC 1 cut(s) 657
TspDTI ATGAA 4 cut(s) 279, 560, 564, 929
TspGWI ACGGA 1 cut(s) 731
TspMI CCCGGG 1 cut(s) 653
TspRI CASTG 2 cut(s) 256, 397
Van91I CCANNNNNTGG 3 cut(s) 207, 839, 973
VpaK11BI GGWCC 3 cut(s) 212, 449, 590
VspI ATTAAT 1 cut(s) 870
XapI RAATTY 3 cut(s) 378, 456, 465
XbaI TCTAGA 1 cut(s) 754
XmaI CCCGGG 1 cut(s) 653
XmiI GTMKAC 1 cut(s) 844
XspI CTAG 5 cut(s) 609, 683, 755, 1053, 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.