Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 516 |
| AccB1I | GGYRCC | 3 cut(s) | 195, 356, 516 |
| AccBSI | CCGCTC | 2 cut(s) | 370, 430 |
| AccI | GTMKAC | 1 cut(s) | 572 |
| AccII | CGCG | 1 cut(s) | 1110 |
| AccIII | TCCGGA | 1 cut(s) | 321 |
| AclWI | GGATC | 3 cut(s) | 447, 780, 1259 |
| AcoI | YGGCCR | 1 cut(s) | 247 |
| AcsI | RAATTY | 3 cut(s) | 125, 343, 410 |
| AcuI | CTGAAG | 1 cut(s) | 1010 |
| AcyI | GRCGYC | 5 cut(s) | 78, 132, 196, 329, 357 |
| AfaI | GTAC | 4 cut(s) | 308, 461, 518, 583 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 149, 479, 510, 527, 1109 |
| AflIII | ACRYGT | 1 cut(s) | 1028 |
| AgeI | ACCGGT | 1 cut(s) | 1117 |
| AgsI | TTSAA | 4 cut(s) | 415, 751, 856, 1067 |
| AjnI | CCWGG | 1 cut(s) | 249 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 858, 890 |
| AluBI | AGCT | 3 cut(s) | 226, 754, 1272 |
| AluI | AGCT | 3 cut(s) | 226, 754, 1272 |
| Alw26I | GTCTC | 3 cut(s) | 389, 733, 1085 |
| AlwI | GGATC | 3 cut(s) | 447, 780, 1259 |
| Ama87I | CYCGRG | 3 cut(s) | 221, 269, 521 |
| Aor13HI | TCCGGA | 1 cut(s) | 321 |
| AoxI | GGCC | 4 cut(s) | 154, 247, 252, 310 |
| ApeKI | GCWGC | 2 cut(s) | 400, 496 |
| ApoI | RAATTY | 3 cut(s) | 125, 343, 410 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 153, 185 |
| AsiGI | ACCGGT | 1 cut(s) | 1117 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 68, 1083 |
| Asp718I | GGTACC | 1 cut(s) | 516 |
| AspLEI | GCGC | 4 cut(s) | 198, 359, 496, 1110 |
| AspS9I | GGNCC | 5 cut(s) | 155, 253, 586, 1019, 1115 |
| AsuC2I | CCSGG | 3 cut(s) | 243, 522, 523 |
| AsuHPI | GGTGA | 4 cut(s) | 139, 226, 682, 964 |
| AsuII | TTCGAA | 3 cut(s) | 38, 264, 827 |
| AvaI | CYCGRG | 3 cut(s) | 221, 269, 521 |
| AvaII | GGWCC | 3 cut(s) | 586, 1019, 1115 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1203, 1236 |
| BanI | GGYRCC | 3 cut(s) | 195, 356, 516 |
| BanII | GRGCYC | 2 cut(s) | 243, 291 |
| BbsI | GAAGAC | 1 cut(s) | 71 |
| BbvI | GCAGC | 2 cut(s) | 387, 483 |
| BccI | CCATC | 2 cut(s) | 445, 1040 |
| BceAI | ACGGC | 3 cut(s) | 112, 325, 397 |
| BciT130I | CCWGG | 1 cut(s) | 251 |
| BciVI | GTATCC | 1 cut(s) | 476 |
| BclI | TGATCA | 1 cut(s) | 954 |
| BcnI | CCSGG | 3 cut(s) | 243, 522, 523 |
| BcoDI | GTCTC | 3 cut(s) | 389, 733, 1085 |
| BfaI | CTAG | 2 cut(s) | 933, 1269 |
| BfoI | RGCGCY | 3 cut(s) | 199, 360, 497 |
| BfuI | GTATCC | 1 cut(s) | 476 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 96 |
| BglII | AGATCT | 1 cut(s) | 1081 |
| Bme1390I | CCNGG | 4 cut(s) | 243, 251, 522, 523 |
| Bme18I | GGWCC | 3 cut(s) | 586, 1019, 1115 |
| BmeT110I | CYCGRG | 3 cut(s) | 221, 269, 521 |
| BmgT120I | GGNCC | 5 cut(s) | 155, 253, 586, 1019, 1115 |
| BmiI | GGNNCC | 5 cut(s) | 157, 197, 358, 518, 1021 |
| BmrFI | CCNGG | 4 cut(s) | 243, 251, 522, 523 |
| BmsI | GCATC | 3 cut(s) | 459, 1165, 1195 |
| BpiI | GAAGAC | 1 cut(s) | 71 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 263, 295, 278, 310 |
| Bpu10I | CCTNAGC | 2 cut(s) | 546, 663 |
| Bpu14I | TTCGAA | 3 cut(s) | 38, 264, 827 |
| BpuEI | CTTGAG | 4 cut(s) | 242, 425, 1109, 1259 |
| BpuMI | CCSGG | 3 cut(s) | 243, 522, 523 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1179 |
| BsaHI | GRCGYC | 5 cut(s) | 78, 132, 196, 329, 357 |
| BsaJI | CCNNGG | 5 cut(s) | 520, 521, 893, 1103, 1276 |
| BsaWI | WCCGGW | 3 cut(s) | 321, 1117, 1259 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 419, 449 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 149, 479, 510, 527, 1109 |
| Bse118I | RCCGGY | 6 cut(s) | 149, 353, 359, 472, 588, 1117 |
| Bse1I | ACTGG | 2 cut(s) | 747, 1195 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1179 |
| BseAI | TCCGGA | 1 cut(s) | 321 |
| BseBI | CCWGG | 1 cut(s) | 251 |
| BseDI | CCNNGG | 5 cut(s) | 520, 521, 893, 1103, 1276 |
| BseGI | GGATG | 7 cut(s) | 456, 474, 811, 1015, 1051, 1180, 1210 |
| BseJI | GATNNNNATC | 1 cut(s) | 1179 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 149, 479, 510, 527, 1109 |
| BseMII | CTCAG | 2 cut(s) | 365, 1242 |
| BseNI | ACTGG | 2 cut(s) | 747, 1195 |
| BseRI | GAGGAG | 7 cut(s) | 407, 470, 668, 862, 895, 910, 925 |
| BseXI | GCAGC | 2 cut(s) | 387, 483 |
| BsgI | GTGCAG | 1 cut(s) | 1080 |
| Bsh1236I | CGCG | 1 cut(s) | 1110 |
| Bsh1285I | CGRYCG | 1 cut(s) | 183 |
| BshFI | GGCC | 4 cut(s) | 156, 249, 254, 312 |
| BshNI | GGYRCC | 3 cut(s) | 195, 356, 516 |
| BshTI | ACCGGT | 1 cut(s) | 1117 |
| BsiEI | CGRYCG | 1 cut(s) | 183 |
| BsiHKCI | CYCGRG | 3 cut(s) | 221, 269, 521 |
| BsiWI | CGTACG | 1 cut(s) | 306 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 149, 479, 510, 527, 1109 |
| BsmAI | GTCTC | 3 cut(s) | 389, 733, 1085 |
| BsmBI | CGTCTC | 1 cut(s) | 389 |
| BsmI | GAATGC | 1 cut(s) | 547 |
| BsnI | GGCC | 4 cut(s) | 156, 249, 254, 312 |
| BsoBI | CYCGRG | 3 cut(s) | 221, 269, 521 |
| Bsp119I | TTCGAA | 3 cut(s) | 38, 264, 827 |
| Bsp1286I | GDGCHC | 2 cut(s) | 243, 291 |
| Bsp13I | TCCGGA | 1 cut(s) | 321 |
| Bsp143I | GATC | 9 cut(s) | 180, 231, 439, 772, 904, 954, 1081, 1180, 1264 |
| BspANI | GGCC | 4 cut(s) | 156, 249, 254, 312 |
| BspCNI | CTCAG | 2 cut(s) | 366, 1243 |
| BspEI | TCCGGA | 1 cut(s) | 321 |
| BspFNI | CGCG | 1 cut(s) | 1110 |
| BspLI | GGNNCC | 5 cut(s) | 157, 197, 358, 518, 1021 |
| BspPI | GGATC | 3 cut(s) | 447, 780, 1259 |
| BspT104I | TTCGAA | 3 cut(s) | 38, 264, 827 |
| BspT107I | GGYRCC | 3 cut(s) | 195, 356, 516 |
| BsrBI | CCGCTC | 2 cut(s) | 370, 430 |
| BsrFI | RCCGGY | 6 cut(s) | 149, 353, 359, 472, 588, 1117 |
| BsrI | ACTGG | 2 cut(s) | 747, 1195 |
| BssAI | RCCGGY | 6 cut(s) | 149, 353, 359, 472, 588, 1117 |
| BssECI | CCNNGG | 5 cut(s) | 520, 521, 893, 1103, 1276 |
| BssMI | GATC | 9 cut(s) | 180, 231, 439, 772, 904, 954, 1081, 1180, 1264 |
| BssNAI | GTATAC | 1 cut(s) | 573 |
| BssNI | GRCGYC | 5 cut(s) | 78, 132, 196, 329, 357 |
| BssT1I | CCWWGG | 2 cut(s) | 1103, 1276 |
| Bst1107I | GTATAC | 1 cut(s) | 573 |
| Bst2UI | CCWGG | 1 cut(s) | 251 |
| Bst4CI | ACNGT | 1 cut(s) | 488 |
| Bst6I | CTCTTC | 2 cut(s) | 40, 831 |
| BstACI | GRCGYC | 5 cut(s) | 78, 132, 196, 329, 357 |
| BstBI | TTCGAA | 3 cut(s) | 38, 264, 827 |
| BstC8I | GCNNGC | 6 cut(s) | 88, 355, 361, 368, 756, 930 |
| BstDEI | CTNAG | 5 cut(s) | 374, 546, 576, 663, 1251 |
| BstF5I | GGATG | 7 cut(s) | 456, 474, 811, 1015, 1051, 1180, 1210 |
| BstFNI | CGCG | 1 cut(s) | 1110 |
| BstH2I | RGCGCY | 3 cut(s) | 199, 360, 497 |
| BstHHI | GCGC | 4 cut(s) | 198, 359, 496, 1110 |
| BstKTI | GATC | 9 cut(s) | 183, 234, 442, 775, 907, 957, 1084, 1183, 1267 |
| BstMAI | GTCTC | 3 cut(s) | 389, 733, 1085 |
| BstMBI | GATC | 9 cut(s) | 180, 231, 439, 772, 904, 954, 1081, 1180, 1264 |
| BstMCI | CGRYCG | 1 cut(s) | 183 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 96, 363, 934 |
| BstNI | CCWGG | 1 cut(s) | 251 |
| BstNSI | RCATGY | 1 cut(s) | 1032 |
| BstSCI | CCNGG | 4 cut(s) | 241, 249, 520, 521 |
| BstUI | CGCG | 1 cut(s) | 1110 |
| BstV1I | GCAGC | 2 cut(s) | 387, 483 |
| BstV2I | GAAGAC | 1 cut(s) | 71 |
| BstX2I | RGATCY | 2 cut(s) | 1081, 1264 |
| BstYI | RGATCY | 2 cut(s) | 1081, 1264 |
| BstZ17I | GTATAC | 1 cut(s) | 573 |
| BsuI | GTATCC | 1 cut(s) | 476 |
| BsuRI | GGCC | 4 cut(s) | 156, 249, 254, 312 |
| BtgZI | GCGATG | 1 cut(s) | 157 |
| BtsCI | GGATG | 7 cut(s) | 456, 474, 811, 1015, 1051, 1180, 1210 |
| Cac8I | GCNNGC | 6 cut(s) | 88, 355, 361, 368, 756, 930 |
| CfoI | GCGC | 4 cut(s) | 198, 359, 496, 1110 |
| Cfr10I | RCCGGY | 6 cut(s) | 149, 353, 359, 472, 588, 1117 |
| Cfr13I | GGNCC | 5 cut(s) | 155, 253, 586, 1019, 1115 |
| Cfr9I | CCCGGG | 1 cut(s) | 521 |
| CpoI | CGGWCCG | 2 cut(s) | 586, 1115 |
| CseI | GACGC | 4 cut(s) | 86, 140, 318, 406 |
| Csp6I | GTAC | 4 cut(s) | 307, 460, 517, 582 |
| CspAI | ACCGGT | 1 cut(s) | 1117 |
| CspI | CGGWCCG | 2 cut(s) | 586, 1115 |
| CviAII | CATG | 2 cut(s) | 1029, 1217 |
| CviQI | GTAC | 4 cut(s) | 307, 460, 517, 582 |
| DdeI | CTNAG | 5 cut(s) | 374, 546, 576, 663, 1251 |
| DinI | GGCGCC | 2 cut(s) | 197, 358 |
| DpnI | GATC | 9 cut(s) | 182, 233, 441, 774, 906, 956, 1083, 1182, 1266 |
| DpnII | GATC | 9 cut(s) | 180, 231, 439, 772, 904, 954, 1081, 1180, 1264 |
| EaeI | YGGCCR | 1 cut(s) | 247 |
| Eam1104I | CTCTTC | 2 cut(s) | 40, 831 |
| EarI | CTCTTC | 2 cut(s) | 40, 831 |
| EciI | GGCGGA | 1 cut(s) | 1128 |
| Eco130I | CCWWGG | 2 cut(s) | 1103, 1276 |
| Eco24I | GRGCYC | 2 cut(s) | 243, 291 |
| Eco47I | GGWCC | 3 cut(s) | 586, 1019, 1115 |
| Eco57I | CTGAAG | 1 cut(s) | 1010 |
| Eco88I | CYCGRG | 3 cut(s) | 221, 269, 521 |
| EcoO109I | RGGNCCY | 1 cut(s) | 253 |
| EcoRI | GAATTC | 2 cut(s) | 125, 343 |
| EcoRII | CCWGG | 1 cut(s) | 249 |
| EcoT14I | CCWWGG | 2 cut(s) | 1103, 1276 |
| EcoT38I | GRGCYC | 2 cut(s) | 243, 291 |
| EgeI | GGCGCC | 2 cut(s) | 197, 358 |
| EheI | GGCGCC | 2 cut(s) | 197, 358 |
| ErhI | CCWWGG | 2 cut(s) | 1103, 1276 |
| Esp3I | CGTCTC | 1 cut(s) | 389 |
| FaeI | CATG | 2 cut(s) | 1032, 1220 |
| FaiI | YATR | 8 cut(s) | 573, 609, 696, 1030, 1037, 1043, 1143, 1218 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1071, 1103 |
| FatI | CATG | 2 cut(s) | 1028, 1216 |
| FauI | CCCGC | 1 cut(s) | 95 |
| FbaI | TGATCA | 1 cut(s) | 954 |
| FblI | GTMKAC | 1 cut(s) | 572 |
| FokI | GGATG | 7 cut(s) | 463, 481, 818, 1022, 1058, 1187, 1217 |
| FriOI | GRGCYC | 2 cut(s) | 243, 291 |
| FspBI | CTAG | 2 cut(s) | 933, 1269 |
| GlaI | GCGC | 4 cut(s) | 197, 358, 495, 1109 |
| HaeII | RGCGCY | 3 cut(s) | 199, 360, 497 |
| HaeIII | GGCC | 4 cut(s) | 156, 249, 254, 312 |
| HgaI | GACGC | 4 cut(s) | 86, 140, 318, 406 |
| HhaI | GCGC | 4 cut(s) | 198, 359, 496, 1110 |
| Hin1I | GRCGYC | 5 cut(s) | 78, 132, 196, 329, 357 |
| Hin1II | CATG | 2 cut(s) | 1032, 1220 |
| Hin6I | GCGC | 4 cut(s) | 196, 357, 494, 1108 |
| HinP1I | GCGC | 4 cut(s) | 196, 357, 494, 1108 |
| HindIII | AAGCTT | 1 cut(s) | 752 |
| HinfI | GANTC | 5 cut(s) | 272, 293, 335, 531, 613 |
| HphI | GGTGA | 4 cut(s) | 139, 226, 682, 964 |
| Hpy166II | GTNNAC | 2 cut(s) | 573, 1257 |
| Hpy188III | TCNNGA | 6 cut(s) | 184, 322, 415, 776, 1088, 1184 |
| Hpy8I | GTNNAC | 2 cut(s) | 573, 1257 |
| Hpy99I | CGWCG | 6 cut(s) | 77, 80, 134, 287, 353, 925 |
| HpyCH4III | ACNGT | 1 cut(s) | 488 |
| HpyCH4IV | ACGT | 1 cut(s) | 318 |
| HpyCH4V | TGCA | 1 cut(s) | 1097 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 96, 363, 934 |
| HpyF3I | CTNAG | 5 cut(s) | 374, 546, 576, 663, 1251 |
| HpySE526I | ACGT | 1 cut(s) | 318 |
| Hsp92I | GRCGYC | 5 cut(s) | 78, 132, 196, 329, 357 |
| Hsp92II | CATG | 2 cut(s) | 1032, 1220 |
| HspAI | GCGC | 4 cut(s) | 196, 357, 494, 1108 |
| KasI | GGCGCC | 2 cut(s) | 195, 356 |
| Kpn2I | TCCGGA | 1 cut(s) | 321 |
| KpnI | GGTACC | 1 cut(s) | 520 |
| KroI | GCCGGC | 2 cut(s) | 353, 359 |
| KroNI | GCCGGC | 2 cut(s) | 355, 361 |
| Ksp22I | TGATCA | 1 cut(s) | 954 |
| Kzo9I | GATC | 9 cut(s) | 180, 231, 439, 772, 904, 954, 1081, 1180, 1264 |
| LmnI | GCTCC | 3 cut(s) | 181, 427, 859 |
| Lsp1109I | GCAGC | 2 cut(s) | 387, 483 |
| LweI | GCATC | 3 cut(s) | 459, 1165, 1195 |
| MaeI | CTAG | 2 cut(s) | 933, 1269 |
| MaeII | ACGT | 1 cut(s) | 318 |
| MalI | GATC | 9 cut(s) | 182, 233, 441, 774, 906, 956, 1083, 1182, 1266 |
| MbiI | CCGCTC | 2 cut(s) | 370, 430 |
| MboI | GATC | 9 cut(s) | 180, 231, 439, 772, 904, 954, 1081, 1180, 1264 |
| MflI | RGATCY | 2 cut(s) | 1081, 1264 |
| MhlI | GDGCHC | 2 cut(s) | 243, 291 |
| MluCI | AATT | 5 cut(s) | 113, 125, 343, 410, 980 |
| Mly113I | GGCGCC | 2 cut(s) | 196, 357 |
| MlyI | GAGTC | 3 cut(s) | 281, 287, 344 |
| MmeI | TCCRAC | 4 cut(s) | 318, 417, 997, 1056 |
| MreI | CGCCGGCG | 2 cut(s) | 353, 359 |
| MroI | TCCGGA | 1 cut(s) | 321 |
| MroNI | GCCGGC | 2 cut(s) | 353, 359 |
| MroXI | GAANNNNTTC | 2 cut(s) | 68, 1083 |
| MseI | TTAA | 1 cut(s) | 792 |
| MspR9I | CCNGG | 4 cut(s) | 243, 251, 522, 523 |
| Mva1269I | GAATGC | 1 cut(s) | 547 |
| MvaI | CCWGG | 1 cut(s) | 251 |
| MvnI | CGCG | 1 cut(s) | 1110 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 96, 363, 934 |
| NaeI | GCCGGC | 2 cut(s) | 355, 361 |
| NarI | GGCGCC | 2 cut(s) | 196, 357 |
| NciI | CCSGG | 3 cut(s) | 243, 522, 523 |
| NdeII | GATC | 9 cut(s) | 180, 231, 439, 772, 904, 954, 1081, 1180, 1264 |
| NgoMIV | GCCGGC | 2 cut(s) | 353, 359 |
| NlaIII | CATG | 2 cut(s) | 1032, 1220 |
| NlaIV | GGNNCC | 5 cut(s) | 157, 197, 358, 518, 1021 |
| NmeAIII | GCCGAG | 1 cut(s) | 918 |
| NspI | RCATGY | 1 cut(s) | 1032 |
| NspV | TTCGAA | 3 cut(s) | 38, 264, 827 |
| PaeR7I | CTCGAG | 1 cut(s) | 221 |
| PciI | ACATGT | 1 cut(s) | 1028 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 180, 315 |
| PctI | GAATGC | 1 cut(s) | 547 |
| PdiI | GCCGGC | 2 cut(s) | 355, 361 |
| PdmI | GAANNNNTTC | 2 cut(s) | 68, 1083 |
| PfeI | GAWTC | 2 cut(s) | 531, 613 |
| Pfl23II | CGTACG | 1 cut(s) | 306 |
| PinAI | ACCGGT | 1 cut(s) | 1117 |
| Ple19I | CGATCG | 1 cut(s) | 183 |
| PleI | GAGTC | 3 cut(s) | 280, 287, 343 |
| PluTI | GGCGCC | 2 cut(s) | 199, 360 |
| PpsI | GAGTC | 3 cut(s) | 280, 287, 343 |
| PscI | ACATGT | 1 cut(s) | 1028 |
| Psp6I | CCWGG | 1 cut(s) | 249 |
| PspGI | CCWGG | 1 cut(s) | 249 |
| PspLI | CGTACG | 1 cut(s) | 306 |
| PspN4I | GGNNCC | 5 cut(s) | 157, 197, 358, 518, 1021 |
| PspPI | GGNCC | 5 cut(s) | 155, 253, 586, 1019, 1115 |
| PspXI | VCTCGAGB | 1 cut(s) | 221 |
| PsuI | RGATCY | 2 cut(s) | 1081, 1264 |
| PvuI | CGATCG | 1 cut(s) | 183 |
| RsaI | GTAC | 4 cut(s) | 308, 461, 518, 583 |
| RsaNI | GTAC | 4 cut(s) | 307, 460, 517, 582 |
| Rsr2I | CGGWCCG | 2 cut(s) | 586, 1115 |
| RsrII | CGGWCCG | 2 cut(s) | 586, 1115 |
| SaqAI | TTAA | 1 cut(s) | 792 |
| Sau3AI | GATC | 9 cut(s) | 180, 231, 439, 772, 904, 954, 1081, 1180, 1264 |
| Sau96I | GGNCC | 5 cut(s) | 155, 253, 586, 1019, 1115 |
| SchI | GAGTC | 3 cut(s) | 281, 287, 344 |
| ScrFI | CCNGG | 4 cut(s) | 243, 251, 522, 523 |
| SduI | GDGCHC | 2 cut(s) | 243, 291 |
| SfaNI | GCATC | 3 cut(s) | 459, 1165, 1195 |
| SfoI | GGCGCC | 2 cut(s) | 197, 358 |
| Sfr274I | CTCGAG | 1 cut(s) | 221 |
| SfuI | TTCGAA | 3 cut(s) | 38, 264, 827 |
| SgrAI | CRCCGGYG | 3 cut(s) | 149, 353, 359 |
| SinI | GGWCC | 3 cut(s) | 586, 1019, 1115 |
| SlaI | CTCGAG | 1 cut(s) | 221 |
| SmaI | CCCGGG | 1 cut(s) | 523 |
| SmlI | CTYRAG | 5 cut(s) | 221, 257, 404, 1088, 1238 |
| SmoI | CTYRAG | 5 cut(s) | 221, 257, 404, 1088, 1238 |
| Sse9I | AATT | 5 cut(s) | 113, 125, 343, 410, 980 |
| SspDI | GGCGCC | 2 cut(s) | 195, 356 |
| SspI | AATATT | 1 cut(s) | 301 |
| SspMI | CTAG | 2 cut(s) | 933, 1269 |
| StyD4I | CCNGG | 4 cut(s) | 241, 249, 520, 521 |
| StyI | CCWWGG | 2 cut(s) | 1103, 1276 |
| TaaI | ACNGT | 1 cut(s) | 488 |
| TaiI | ACGT | 1 cut(s) | 321 |
| TasI | AATT | 5 cut(s) | 113, 125, 343, 410, 980 |
| TauI | GCSGC | 8 cut(s) | 102, 156, 249, 366, 373, 387, 494, 1113 |
| TfiI | GAWTC | 2 cut(s) | 531, 613 |
| Tru1I | TTAA | 1 cut(s) | 792 |
| Tru9I | TTAA | 1 cut(s) | 792 |
| TseI | GCWGC | 2 cut(s) | 400, 496 |
| TspDTI | ATGAA | 3 cut(s) | 17, 227, 596 |
| TspGWI | ACGGA | 3 cut(s) | 271, 337, 599 |
| TspMI | CCCGGG | 1 cut(s) | 521 |
| VpaK11BI | GGWCC | 3 cut(s) | 586, 1019, 1115 |
| XapI | RAATTY | 3 cut(s) | 125, 343, 410 |
| XceI | RCATGY | 1 cut(s) | 1032 |
| XhoI | CTCGAG | 1 cut(s) | 221 |
| XmaI | CCCGGG | 1 cut(s) | 521 |
| XmiI | GTMKAC | 1 cut(s) | 572 |
| XmnI | GAANNNNTTC | 2 cut(s) | 68, 1083 |
| XspI | CTAG | 2 cut(s) | 933, 1269 |