Domain of unknown function (DUF4220)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 157, 1050 |
| AcoI | YGGCCR | 1 cut(s) | 894 |
| AcsI | RAATTY | 3 cut(s) | 172, 832, 942 |
| AcuI | CTGAAG | 3 cut(s) | 669, 771, 1056 |
| AfaI | GTAC | 1 cut(s) | 237 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 146, 147 |
| AgsI | TTSAA | 6 cut(s) | 66, 354, 405, 576, 679, 984 |
| AjnI | CCWGG | 2 cut(s) | 482, 504 |
| AluBI | AGCT | 8 cut(s) | 189, 442, 614, 749, 785, 859, 868, 1127 |
| AluI | AGCT | 8 cut(s) | 189, 442, 614, 749, 785, 859, 868, 1127 |
| Alw26I | GTCTC | 3 cut(s) | 9, 63, 90 |
| Ama87I | CYCGRG | 1 cut(s) | 190 |
| AoxI | GGCC | 2 cut(s) | 536, 894 |
| ApeKI | GCWGC | 5 cut(s) | 165, 264, 614, 746, 1055 |
| ApoI | RAATTY | 3 cut(s) | 172, 832, 942 |
| AspLEI | GCGC | 1 cut(s) | 264 |
| AsuC2I | CCSGG | 2 cut(s) | 33, 535 |
| AsuHPI | GGTGA | 2 cut(s) | 131, 137 |
| AvaI | CYCGRG | 1 cut(s) | 190 |
| BalI | TGGCCA | 1 cut(s) | 896 |
| BbsI | GAAGAC | 2 cut(s) | 689, 1015 |
| BbvI | GCAGC | 5 cut(s) | 177, 276, 601, 758, 1067 |
| BccI | CCATC | 3 cut(s) | 104, 554, 652 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 171, 205 |
| BciT130I | CCWGG | 2 cut(s) | 484, 506 |
| BcnI | CCSGG | 2 cut(s) | 33, 535 |
| BcoDI | GTCTC | 3 cut(s) | 9, 63, 90 |
| BfaI | CTAG | 1 cut(s) | 540 |
| BfmI | CTRYAG | 2 cut(s) | 780, 1122 |
| BglII | AGATCT | 3 cut(s) | 545, 910, 1134 |
| BisI | GCNGC | 5 cut(s) | 166, 265, 615, 747, 1056 |
| BlsI | GCNGC | 5 cut(s) | 167, 266, 616, 748, 1057 |
| Bme1390I | CCNGG | 4 cut(s) | 33, 484, 506, 535 |
| BmeT110I | CYCGRG | 1 cut(s) | 190 |
| BmrFI | CCNGG | 4 cut(s) | 33, 484, 506, 535 |
| BmsI | GCATC | 2 cut(s) | 191, 766 |
| BpiI | GAAGAC | 2 cut(s) | 689, 1015 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 70, 102 |
| BpmI | CTGGAG | 1 cut(s) | 719 |
| Bpu10I | CCTNAGC | 1 cut(s) | 258 |
| BpuEI | CTTGAG | 1 cut(s) | 1103 |
| BpuMI | CCSGG | 2 cut(s) | 33, 535 |
| Bsa29I | ATCGAT | 1 cut(s) | 1104 |
| BsaJI | CCNNGG | 2 cut(s) | 482, 504 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 146, 147 |
| Bse1I | ACTGG | 1 cut(s) | 1009 |
| BseBI | CCWGG | 2 cut(s) | 484, 506 |
| BseCI | ATCGAT | 1 cut(s) | 1104 |
| BseDI | CCNNGG | 2 cut(s) | 482, 504 |
| BseGI | GGATG | 6 cut(s) | 63, 115, 182, 565, 811, 976 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 146, 147 |
| BseMII | CTCAG | 1 cut(s) | 249 |
| BseNI | ACTGG | 1 cut(s) | 1009 |
| BseRI | GAGGAG | 1 cut(s) | 869 |
| BseXI | GCAGC | 5 cut(s) | 177, 276, 601, 758, 1067 |
| BseYI | CCCAGC | 1 cut(s) | 473 |
| BsgI | GTGCAG | 1 cut(s) | 1074 |
| BshFI | GGCC | 2 cut(s) | 538, 896 |
| BshVI | ATCGAT | 1 cut(s) | 1104 |
| BsiHKCI | CYCGRG | 1 cut(s) | 190 |
| BsiSI | CCGG | 2 cut(s) | 33, 535 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 146, 147 |
| BsmAI | GTCTC | 3 cut(s) | 9, 63, 90 |
| BsnI | GGCC | 2 cut(s) | 538, 896 |
| BsoBI | CYCGRG | 1 cut(s) | 190 |
| Bsp143I | GATC | 4 cut(s) | 545, 910, 1105, 1134 |
| BspACI | CCGC | 2 cut(s) | 157, 1050 |
| BspANI | GGCC | 2 cut(s) | 538, 896 |
| BspCNI | CTCAG | 1 cut(s) | 250 |
| BspDI | ATCGAT | 1 cut(s) | 1104 |
| BsrI | ACTGG | 1 cut(s) | 1009 |
| BssECI | CCNNGG | 2 cut(s) | 482, 504 |
| BssMI | GATC | 4 cut(s) | 545, 910, 1105, 1134 |
| Bst2UI | CCWGG | 2 cut(s) | 484, 506 |
| Bst4CI | ACNGT | 2 cut(s) | 276, 935 |
| Bst6I | CTCTTC | 1 cut(s) | 1089 |
| BstC8I | GCNNGC | 2 cut(s) | 471, 861 |
| BstDEI | CTNAG | 2 cut(s) | 258, 363 |
| BstF5I | GGATG | 6 cut(s) | 63, 115, 182, 565, 811, 976 |
| BstHHI | GCGC | 1 cut(s) | 264 |
| BstKTI | GATC | 4 cut(s) | 548, 913, 1108, 1137 |
| BstMAI | GTCTC | 3 cut(s) | 9, 63, 90 |
| BstMBI | GATC | 4 cut(s) | 545, 910, 1105, 1134 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 439, 865 |
| BstNI | CCWGG | 2 cut(s) | 484, 506 |
| BstNSI | RCATGY | 1 cut(s) | 473 |
| BstSCI | CCNGG | 4 cut(s) | 31, 482, 504, 533 |
| BstSFI | CTRYAG | 2 cut(s) | 780, 1122 |
| BstV1I | GCAGC | 5 cut(s) | 177, 276, 601, 758, 1067 |
| BstV2I | GAAGAC | 2 cut(s) | 689, 1015 |
| BstX2I | RGATCY | 3 cut(s) | 545, 910, 1134 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 217 |
| BstYI | RGATCY | 3 cut(s) | 545, 910, 1134 |
| Bsu15I | ATCGAT | 1 cut(s) | 1104 |
| BsuRI | GGCC | 2 cut(s) | 538, 896 |
| BsuTUI | ATCGAT | 1 cut(s) | 1104 |
| BtsCI | GGATG | 6 cut(s) | 63, 115, 182, 565, 811, 976 |
| BtsI | GCAGTG | 1 cut(s) | 502 |
| BtsIMutI | CAGTG | 1 cut(s) | 502 |
| Cac8I | GCNNGC | 2 cut(s) | 471, 861 |
| CfoI | GCGC | 1 cut(s) | 264 |
| ClaI | ATCGAT | 1 cut(s) | 1104 |
| Csp6I | GTAC | 1 cut(s) | 236 |
| CviAII | CATG | 4 cut(s) | 54, 470, 693, 845 |
| CviQI | GTAC | 1 cut(s) | 236 |
| DdeI | CTNAG | 2 cut(s) | 258, 363 |
| DpnI | GATC | 4 cut(s) | 547, 912, 1107, 1136 |
| DpnII | GATC | 4 cut(s) | 545, 910, 1105, 1134 |
| DraI | TTTAAA | 1 cut(s) | 600 |
| EaeI | YGGCCR | 1 cut(s) | 894 |
| Eam1104I | CTCTTC | 1 cut(s) | 1089 |
| EarI | CTCTTC | 1 cut(s) | 1089 |
| Eco32I | GATATC | 2 cut(s) | 417, 690 |
| Eco57I | CTGAAG | 3 cut(s) | 669, 771, 1056 |
| Eco88I | CYCGRG | 1 cut(s) | 190 |
| EcoRI | GAATTC | 1 cut(s) | 172 |
| EcoRII | CCWGG | 2 cut(s) | 482, 504 |
| EcoRV | GATATC | 2 cut(s) | 417, 690 |
| EcoT22I | ATGCAT | 1 cut(s) | 471 |
| FaeI | CATG | 4 cut(s) | 57, 473, 696, 848 |
| FatI | CATG | 4 cut(s) | 53, 469, 692, 844 |
| FauI | CCCGC | 1 cut(s) | 150 |
| FauNDI | CATATG | 1 cut(s) | 727 |
| Fnu4HI | GCNGC | 5 cut(s) | 166, 265, 615, 747, 1056 |
| FokI | GGATG | 6 cut(s) | 70, 122, 169, 572, 818, 983 |
| Fsp4HI | GCNGC | 5 cut(s) | 166, 265, 615, 747, 1056 |
| FspBI | CTAG | 1 cut(s) | 540 |
| GlaI | GCGC | 1 cut(s) | 263 |
| GluI | GCNGC | 5 cut(s) | 166, 265, 615, 747, 1056 |
| GsaI | CCCAGC | 1 cut(s) | 477 |
| GsuI | CTGGAG | 1 cut(s) | 719 |
| HaeIII | GGCC | 2 cut(s) | 538, 896 |
| HapII | CCGG | 2 cut(s) | 33, 535 |
| HhaI | GCGC | 1 cut(s) | 264 |
| Hin1II | CATG | 4 cut(s) | 57, 473, 696, 848 |
| Hin6I | GCGC | 1 cut(s) | 262 |
| HinP1I | GCGC | 1 cut(s) | 262 |
| HindIII | AAGCTT | 1 cut(s) | 440 |
| HinfI | GANTC | 5 cut(s) | 83, 566, 620, 703, 977 |
| HpaII | CCGG | 2 cut(s) | 33, 535 |
| HphI | GGTGA | 2 cut(s) | 131, 137 |
| Hpy166II | GTNNAC | 2 cut(s) | 770, 938 |
| Hpy188I | TCNGA | 3 cut(s) | 325, 421, 649 |
| Hpy188III | TCNNGA | 7 cut(s) | 66, 89, 192, 218, 257, 707, 791 |
| Hpy8I | GTNNAC | 2 cut(s) | 770, 938 |
| HpyAV | CCTTC | 7 cut(s) | 56, 60, 116, 215, 582, 746, 1012 |
| HpyCH4III | ACNGT | 2 cut(s) | 276, 935 |
| HpyCH4V | TGCA | 8 cut(s) | 182, 330, 469, 593, 863, 918, 1055, 1112 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 439, 865 |
| HpyF3I | CTNAG | 2 cut(s) | 258, 363 |
| Hsp92II | CATG | 4 cut(s) | 57, 473, 696, 848 |
| HspAI | GCGC | 1 cut(s) | 262 |
| Kzo9I | GATC | 4 cut(s) | 545, 910, 1105, 1134 |
| LmnI | GCTCC | 2 cut(s) | 296, 856 |
| Lsp1109I | GCAGC | 5 cut(s) | 177, 276, 601, 758, 1067 |
| LweI | GCATC | 2 cut(s) | 191, 766 |
| MaeI | CTAG | 1 cut(s) | 540 |
| MaeIII | GTNAC | 3 cut(s) | 276, 425, 458 |
| MalI | GATC | 4 cut(s) | 547, 912, 1107, 1136 |
| MboI | GATC | 4 cut(s) | 545, 910, 1105, 1134 |
| MflI | RGATCY | 3 cut(s) | 545, 910, 1134 |
| MlsI | TGGCCA | 1 cut(s) | 896 |
| MluCI | AATT | 4 cut(s) | 172, 832, 942, 964 |
| MluNI | TGGCCA | 1 cut(s) | 896 |
| MlyI | GAGTC | 3 cut(s) | 92, 614, 712 |
| MmeI | TCCRAC | 1 cut(s) | 267 |
| MnlI | CCTC | 8 cut(s) | 121, 146, 373, 598, 847, 852, 943, 1090 |
| Mox20I | TGGCCA | 1 cut(s) | 896 |
| Mph1103I | ATGCAT | 1 cut(s) | 471 |
| MscI | TGGCCA | 1 cut(s) | 896 |
| MseI | TTAA | 3 cut(s) | 252, 599, 761 |
| Msp20I | TGGCCA | 1 cut(s) | 896 |
| MspA1I | CMGCKG | 2 cut(s) | 749, 1050 |
| MspI | CCGG | 2 cut(s) | 33, 535 |
| MspR9I | CCNGG | 4 cut(s) | 33, 484, 506, 535 |
| MvaI | CCWGG | 2 cut(s) | 484, 506 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 439, 865 |
| NciI | CCSGG | 2 cut(s) | 33, 535 |
| NdeI | CATATG | 1 cut(s) | 727 |
| NdeII | GATC | 4 cut(s) | 545, 910, 1105, 1134 |
| NlaIII | CATG | 4 cut(s) | 57, 473, 696, 848 |
| NmuCI | GTSAC | 1 cut(s) | 276 |
| NsiI | ATGCAT | 1 cut(s) | 471 |
| NspI | RCATGY | 1 cut(s) | 473 |
| PaeI | GCATGC | 1 cut(s) | 473 |
| PfeI | GAWTC | 2 cut(s) | 566, 977 |
| PkrI | GCNGC | 5 cut(s) | 167, 266, 616, 748, 1057 |
| PleI | GAGTC | 3 cut(s) | 91, 614, 711 |
| PpsI | GAGTC | 3 cut(s) | 91, 614, 711 |
| Psp6I | CCWGG | 2 cut(s) | 482, 504 |
| PspFI | CCCAGC | 1 cut(s) | 473 |
| PspGI | CCWGG | 2 cut(s) | 482, 504 |
| PsuI | RGATCY | 3 cut(s) | 545, 910, 1134 |
| PvuII | CAGCTG | 1 cut(s) | 749 |
| RsaI | GTAC | 1 cut(s) | 237 |
| RsaNI | GTAC | 1 cut(s) | 236 |
| SaqAI | TTAA | 3 cut(s) | 252, 599, 761 |
| SatI | GCNGC | 5 cut(s) | 166, 265, 615, 747, 1056 |
| Sau3AI | GATC | 4 cut(s) | 545, 910, 1105, 1134 |
| SchI | GAGTC | 3 cut(s) | 92, 614, 712 |
| ScrFI | CCNGG | 4 cut(s) | 33, 484, 506, 535 |
| SfaNI | GCATC | 2 cut(s) | 191, 766 |
| SfcI | CTRYAG | 2 cut(s) | 780, 1122 |
| SmlI | CTYRAG | 1 cut(s) | 1082 |
| SmoI | CTYRAG | 1 cut(s) | 1082 |
| SphI | GCATGC | 1 cut(s) | 473 |
| Sse9I | AATT | 4 cut(s) | 172, 832, 942, 964 |
| SsiI | CCGC | 2 cut(s) | 157, 1050 |
| SspMI | CTAG | 1 cut(s) | 540 |
| StyD4I | CCNGG | 4 cut(s) | 31, 482, 504, 533 |
| TaaI | ACNGT | 2 cut(s) | 276, 935 |
| TaqI | TCGA | 4 cut(s) | 370, 669, 870, 1104 |
| TasI | AATT | 4 cut(s) | 172, 832, 942, 964 |
| TatI | WGTACW | 1 cut(s) | 235 |
| TfiI | GAWTC | 2 cut(s) | 566, 977 |
| Tru1I | TTAA | 3 cut(s) | 252, 599, 761 |
| Tru9I | TTAA | 3 cut(s) | 252, 599, 761 |
| TscAI | CASTG | 1 cut(s) | 502 |
| TseFI | GTSAC | 1 cut(s) | 276 |
| TseI | GCWGC | 5 cut(s) | 165, 264, 614, 746, 1055 |
| Tsp45I | GTSAC | 1 cut(s) | 276 |
| TspDTI | ATGAA | 4 cut(s) | 42, 826, 950, 1089 |
| TspRI | CASTG | 1 cut(s) | 502 |
| XapI | RAATTY | 3 cut(s) | 172, 832, 942 |
| XceI | RCATGY | 1 cut(s) | 473 |
| XspI | CTAG | 1 cut(s) | 540 |
| Zsp2I | ATGCAT | 1 cut(s) | 471 |