FvH4_6g34780

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000618
Physical Location & Seq
Reverse (-)
27430679 .. 27434990
4312 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g34780.t1

Sequence Viewer

Length: 2112 bp
ATGTCAGTTACAATTGTCAGCAGCTTGATCTTCGCAAACAAGAGGAAGGCAATGCAACTCTTCCCAAAAAGATTGACGATAGTCTGGAATGAGTGGGAGCTCCGGGTTATGGTACTAATCAGCCTTTGCCTCCAAGTAGTCCTCATTCTGATTGGCAACTGGAGAAAACATTCAACCAGTAACAAACTCAGAATTGTTTTGTGGCTCGCTTACTTGTCGGCGGACTCAGTTGCATCGGTTTCACTTGGCATACTCTGCAACAGCGAAGAAGACCCTGAAGGTGATTCTCAACACCCAAACAGCTTTATAATAACAGCATTTTGGGCACCCTTTCTTCTGTTGCATCTTGGTGGCCCAGATACTATTACTGCTTACTCATTAGAAGACAATGAGTTGTGGCTGAGGCACTTGCTTGGGCTAGTTGTCCAGGTGTCTGTAGCTGTTTATGTCTTCCTCAGAGCATGGTCTAGTAAAATGTTGAATTTTTTGGCAATACCAATCTTCATAGTTGGTGTTATAAAGTTTGGGGAGAGGACTTGGGTTCTAAGATCTGCGAGCAGTGAGCATTTTAGAGATTCTATGCTCCAGGATCCTGACCCAGGTCCCAATTATGCTAGATACATGGAAGAATACAGTTCAAAGAGATCTGAGGGCTTCAGGGTTGAACTAGGGACATTCGTTGAAGCTCCCCTAGTTGGTAATCACTTGGATCCTGCTGCAGCCATCGACAGTAATCAAAATGCTTCAAGTTTAAGCAGAGCCTACAAGTTCTCAGAAACTTTTAAACGGCTATGTGCTGATCTCATACTCAGCTTCCATGACATAGTAAATAGCCAATCCTTCTTCCAAACTAGAAGCTCTGAGGAAGCTTTTGAAGTTATTGAGTTTGAGCTGGGTTTCTTATATGATGTGTTTTATACTAAAGCAGTCCTGGTTTACTCTGGTCTTGGTAGAATTCTTCGCTGCATCACTCTCATTTTAACAGTTGTTGTATTTGTAGCCTTCCTTCTTGAAAAGAAGCAAGCATTCAAGGGAGTGGATGTAATAATCACCTATATATTGTTGGGAGGAGCTATCATCCTAGAAATGTATTCTGTAGTCCTACTGCTTTCCTCAGACTGGACAAGGCTATGGCTCAGTAGGCACCACAATGTGGCAGTGAGTCTCTTGTACAGACTCGTTTCGTCAATCCCATTGGTTAAGAACAAGAGCTGGTCTAATACAATAGCACAATACAATCTAATAACATTTTGTCTTAAAGCTAGGCCAGCAAATTGTACTTTTCTCAAGAAGGATTTCTTCCTGAATCGGTTGTTAGAGAGGTACAGGTACAGAGACTTGAAGGGTGTTTCCAAAGAGTTGAAGAATATGATATTTGAGCAGCTACAACAGAAATCAAGAAGTGCTTCCAATTTCGTGGCTTGCAAACAATTGTGTGCTCGAAGAGGTGACTGGGTGCTTGAAAACGAAGGTTGTCTTGACAAACTTGGCTGGAGCATTGATGAAGTTGAGTTTGACCAAAGCATCCTTCTCTGGCATATTGCAACAGATATCTGTTATCATTTTGATCTAAACAGAAACTTAAACCCTGATAGTAACACAAACTGTGAAGCCAGCAAGCTGTTATCCAACTACATGTTGTATCTTCTAGTGATGTGCCCCTTCATGCTACCCAACGGGATTGGATTGATAAGGTTTAGGGACACTTGTGCTGAGGCTGAAGAATTTTTCAAAGAAAAAAAGATAATGAAATCAGAGACAAAGGAAGCTTGCACAAAGCTTTATAACGTGTGTACAGACATCCTTCCATCCAAAGTGAAGGGAGACAGAAGCAAGTCGGTTCTGTTTGATGCATGCCGGCTTGCTAAAGCTTTGCAGTCACTTAAAACAGAGGAAAATTGGTATAATCAGAAGTGGGAGTTGATGAGTCAAGTTTGGGTGGAAATGCTCTCTTACGCGGCCAATCAGTGTCGATGGGGTGATCATGCTCAGCAGCTCAGGCGAGGAGGAGAACTTCTTACTCATGTCTGGCTTCTTATGGCACATCTTGGTTTAACTGAACAGTTCCAAATTTCTGAGGGCCATGTTAGGGCAAAATTGGTTGTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

704

Amino Acids

80.65

Weight (kDa)

8.18

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 68 - 416 1e-110 Domain of unknown function (DUF4220)
DUF594 PF04578 635 - 685 3.1e-23 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 308, 518, 1785
AccB1I GGYRCC 2 cut(s) 325, 1143
AccB7I CCANNNNNTGG 1 cut(s) 1153
AccII CGCG 1 cut(s) 1958
AciI CCGC 2 cut(s) 221, 1958
AclWI GGATC 4 cut(s) 584, 597, 704, 717
AcoI YGGCCR 1 cut(s) 1959
AcsI RAATTY 4 cut(s) 481, 954, 1724, 2070
AcuI CTGAAG 3 cut(s) 297, 640, 1740
AdeI CACNNNGTG 1 cut(s) 1153
AfaI GTAC 6 cut(s) 114, 1172, 1279, 1325, 1331, 1795
AfiI CCNNNNNNNGG 6 cut(s) 109, 599, 695, 1119, 1153, 2089
AflIII ACRYGT 2 cut(s) 1635, 1788
AjnI CCWGG 4 cut(s) 426, 585, 598, 930
AjuI GAANNNNNNNTTGG 2 cut(s) 1512, 1544
Alw21I GWGCWC 2 cut(s) 102, 1441
Alw26I GTCTC 4 cut(s) 1169, 1329, 1751, 1818
AlwI GGATC 4 cut(s) 584, 597, 704, 717
AoxI GGCC 4 cut(s) 352, 1265, 1959, 2080
ApeKI GCWGC 6 cut(s) 21, 716, 719, 963, 1381, 1993
ApoI RAATTY 4 cut(s) 481, 954, 1724, 2070
Asp700I GAANNNNTTC 3 cut(s) 169, 1295, 1405
AspS9I GGNCC 3 cut(s) 353, 602, 2080
AsuC2I CCSGG 1 cut(s) 104
AsuHPI GGTGA 4 cut(s) 293, 1042, 1460, 1991
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 2 cut(s) 328, 1661
BamHI GGATCC 2 cut(s) 589, 709
BanI GGYRCC 2 cut(s) 325, 1143
BanII GRGCYC 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 990, 1022
BbsI GAAGAC 3 cut(s) 276, 390, 442
Bbv12I GWGCWC 2 cut(s) 102, 1441
BbvCI CCTCAGC 2 cut(s) 401, 1713
BbvI GCAGC 6 cut(s) 33, 703, 731, 950, 1393, 2005
BccI CCATC 3 cut(s) 731, 1816, 1968
BceAI ACGGC 1 cut(s) 803
BciT130I CCWGG 4 cut(s) 428, 587, 600, 932
BclI TGATCA 1 cut(s) 1981
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 4 cut(s) 1169, 1329, 1751, 1818
BfaI CTAG 9 cut(s) 419, 468, 615, 668, 692, 852, 1082, 1263, 1649
BfmI CTRYAG 3 cut(s) 435, 717, 1095
BglII AGATCT 2 cut(s) 548, 644
BisI GCNGC 7 cut(s) 22, 717, 720, 964, 1382, 1959, 1994
BlpI GCTNAGC 1 cut(s) 1989
BlsI GCNGC 7 cut(s) 23, 718, 721, 965, 1383, 1960, 1995
Bme1390I CCNGG 5 cut(s) 104, 428, 587, 600, 932
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 3 cut(s) 353, 602, 2080
BmiI GGNNCC 5 cut(s) 327, 591, 604, 711, 1145
BmrFI CCNGG 5 cut(s) 104, 428, 587, 600, 932
BmrI ACTGGG 1 cut(s) 1462
BmsI GCATC 5 cut(s) 242, 352, 975, 1533, 1840
BmuI ACTGGG 1 cut(s) 1462
BoxI GACNNNNGTC 2 cut(s) 80, 600
BpiI GAAGAC 3 cut(s) 276, 390, 442
BpmI CTGGAG 3 cut(s) 181, 569, 1513
Bpu10I CCTNAGC 3 cut(s) 401, 1713, 1997
Bpu1102I GCTNAGC 1 cut(s) 1989
BpuEI CTTGAG 1 cut(s) 1271
BpuMI CCSGG 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 598
BsaXI ACNNNNNCTCC 2 cut(s) 1026, 1056
Bsc4I CCNNNNNNNGG 6 cut(s) 109, 599, 695, 1119, 1153, 2089
Bse118I RCCGGY 1 cut(s) 1857
Bse1I ACTGG 4 cut(s) 164, 177, 1124, 1457
Bse3DI GCAATG 1 cut(s) 57
BseBI CCWGG 4 cut(s) 428, 587, 600, 932
BseDI CCNNGG 1 cut(s) 598
BseGI GGATG 5 cut(s) 1045, 1077, 1524, 1800, 1808
BseLI CCNNNNNNNGG 6 cut(s) 109, 599, 695, 1119, 1153, 2089
BseMI GCAATG 1 cut(s) 57
BseNI ACTGG 4 cut(s) 164, 177, 1124, 1457
BseRI GAGGAG 3 cut(s) 1083, 2019, 2022
BseSI GKGCMC 2 cut(s) 328, 1661
BseXI GCAGC 6 cut(s) 33, 703, 731, 950, 1393, 2005
BseYI CCCAGC 1 cut(s) 892
Bsh1236I CGCG 1 cut(s) 1958
BshFI GGCC 4 cut(s) 354, 1267, 1961, 2082
BshNI GGYRCC 2 cut(s) 325, 1143
BsiHKAI GWGCWC 2 cut(s) 102, 1441
BsiSI CCGG 2 cut(s) 103, 1858
BslFI GGGAC 3 cut(s) 588, 685, 1715
BslI CCNNNNNNNGG 6 cut(s) 109, 599, 695, 1119, 1153, 2089
BsmAI GTCTC 4 cut(s) 1169, 1329, 1751, 1818
BsmFI GGGAC 3 cut(s) 588, 685, 1715
BsmI GAATGC 1 cut(s) 1025
BsnI GGCC 4 cut(s) 354, 1267, 1961, 2082
Bsp1286I GDGCHC 4 cut(s) 102, 328, 1441, 1661
Bsp1407I TGTACA 2 cut(s) 1170, 1793
Bsp143I GATC 8 cut(s) 27, 548, 589, 644, 709, 799, 1567, 1981
Bsp1720I GCTNAGC 1 cut(s) 1989
BspACI CCGC 2 cut(s) 221, 1958
BspANI GGCC 4 cut(s) 354, 1267, 1961, 2082
BspFNI CGCG 1 cut(s) 1958
BspLI GGNNCC 5 cut(s) 327, 591, 604, 711, 1145
BspMAI CTGCAG 1 cut(s) 721
BspPI GGATC 4 cut(s) 584, 597, 704, 717
BspT107I GGYRCC 2 cut(s) 325, 1143
BsrDI GCAATG 1 cut(s) 57
BsrFI RCCGGY 1 cut(s) 1857
BsrGI TGTACA 2 cut(s) 1170, 1793
BsrI ACTGG 4 cut(s) 164, 177, 1124, 1457
BssAI RCCGGY 1 cut(s) 1857
BssECI CCNNGG 1 cut(s) 598
BssMI GATC 8 cut(s) 27, 548, 589, 644, 709, 799, 1567, 1981
Bst2UI CCWGG 4 cut(s) 428, 587, 600, 932
Bst4CI ACNGT 5 cut(s) 635, 731, 985, 1607, 2064
Bst6I CTCTTC 2 cut(s) 65, 1438
BstAPI GCANNNNNTGC 1 cut(s) 255
BstAUI TGTACA 2 cut(s) 1170, 1793
BstENI CCTNNNNNAGG 1 cut(s) 597
BstF5I GGATG 5 cut(s) 1045, 1077, 1524, 1800, 1808
BstFNI CGCG 1 cut(s) 1958
BstKTI GATC 8 cut(s) 30, 551, 592, 647, 712, 802, 1570, 1984
BstMAI GTCTC 4 cut(s) 1169, 1329, 1751, 1818
BstMBI GATC 8 cut(s) 27, 548, 589, 644, 709, 799, 1567, 1981
BstMWI GCNNNNNNNGC 5 cut(s) 255, 323, 1141, 1268, 1999
BstNI CCWGG 4 cut(s) 428, 587, 600, 932
BstNSI RCATGY 2 cut(s) 1639, 1857
BstPAI GACNNNNGTC 2 cut(s) 80, 600
BstSCI CCNGG 5 cut(s) 102, 426, 585, 598, 930
BstSFI CTRYAG 3 cut(s) 435, 717, 1095
BstSLI GKGCMC 2 cut(s) 328, 1661
BstUI CGCG 1 cut(s) 1958
BstV1I GCAGC 6 cut(s) 33, 703, 731, 950, 1393, 2005
BstV2I GAAGAC 3 cut(s) 276, 390, 442
BstX2I RGATCY 4 cut(s) 548, 589, 644, 709
BstXI CCANNNNNNTGG 1 cut(s) 1417
BstYI RGATCY 4 cut(s) 548, 589, 644, 709
BsuRI GGCC 4 cut(s) 354, 1267, 1961, 2082
BtsCI GGATG 5 cut(s) 1045, 1077, 1524, 1800, 1808
BtsI GCAGTG 2 cut(s) 565, 1164
BtsIMutI CAGTG 3 cut(s) 565, 1164, 1973
Cfr10I RCCGGY 1 cut(s) 1857
Cfr13I GGNCC 3 cut(s) 353, 602, 2080
Csp6I GTAC 6 cut(s) 113, 1171, 1278, 1324, 1330, 1794
CviAII CATG 9 cut(s) 462, 622, 818, 1636, 1666, 1854, 1985, 2024, 2084
CviQI GTAC 6 cut(s) 113, 1171, 1278, 1324, 1330, 1794
DpnI GATC 8 cut(s) 29, 550, 591, 646, 711, 801, 1569, 1983
DpnII GATC 8 cut(s) 27, 548, 589, 644, 709, 799, 1567, 1981
DraI TTTAAA 1 cut(s) 784
DraIII CACNNNGTG 1 cut(s) 1153
EaeI YGGCCR 1 cut(s) 1959
Eam1104I CTCTTC 2 cut(s) 65, 1438
EarI CTCTTC 2 cut(s) 65, 1438
EciI GGCGGA 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 102
Eco32I GATATC 1 cut(s) 1552
Eco47I GGWCC 1 cut(s) 602
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 3 cut(s) 297, 640, 1740
EcoICRI GAGCTC 1 cut(s) 100
EcoNI CCTNNNNNAGG 1 cut(s) 597
EcoO109I RGGNCCY 1 cut(s) 602
EcoRI GAATTC 1 cut(s) 954
EcoRII CCWGG 4 cut(s) 426, 585, 598, 930
EcoRV GATATC 1 cut(s) 1552
EcoT22I ATGCAT 1 cut(s) 1855
EcoT38I GRGCYC 1 cut(s) 102
FaeI CATG 9 cut(s) 465, 625, 821, 1639, 1669, 1857, 1988, 2027, 2087
FalI AAGNNNNNCTT 6 cut(s) 1283, 1315, 1390, 1422, 1461, 1493
FaqI GGGAC 3 cut(s) 588, 685, 1715
FatI CATG 9 cut(s) 461, 621, 817, 1635, 1665, 1853, 1984, 2023, 2083
FbaI TGATCA 1 cut(s) 1981
Fnu4HI GCNGC 7 cut(s) 22, 717, 720, 964, 1382, 1959, 1994
FokI GGATG 5 cut(s) 1052, 1064, 1511, 1787, 1795
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 7 cut(s) 22, 717, 720, 964, 1382, 1959, 1994
FspBI CTAG 9 cut(s) 419, 468, 615, 668, 692, 852, 1082, 1263, 1649
GluI GCNGC 7 cut(s) 22, 717, 720, 964, 1382, 1959, 1994
GsaI CCCAGC 1 cut(s) 896
GsuI CTGGAG 3 cut(s) 181, 569, 1513
HaeIII GGCC 4 cut(s) 354, 1267, 1961, 2082
HapII CCGG 2 cut(s) 103, 1858
Hin1II CATG 9 cut(s) 465, 625, 821, 1639, 1669, 1857, 1988, 2027, 2087
HindIII AAGCTT 4 cut(s) 867, 1767, 1778, 1869
HinfI GANTC 7 cut(s) 224, 284, 575, 1162, 1176, 1306, 1927
HpaII CCGG 2 cut(s) 103, 1858
HphI GGTGA 4 cut(s) 293, 1042, 1460, 1991
Hpy166II GTNNAC 2 cut(s) 937, 1794
Hpy188III TCNNGA 7 cut(s) 85, 593, 1010, 1288, 1303, 1398, 1478
Hpy8I GTNNAC 2 cut(s) 937, 1794
HpyCH4III ACNGT 5 cut(s) 635, 731, 985, 1607, 2064
HpyCH4IV ACGT 1 cut(s) 1788
HpyF10VI GCNNNNNNNGC 5 cut(s) 255, 323, 1141, 1268, 1999
HpySE526I ACGT 1 cut(s) 1788
Hsp92II CATG 9 cut(s) 465, 625, 821, 1639, 1669, 1857, 1988, 2027, 2087
KroI GCCGGC 1 cut(s) 1857
KroNI GCCGGC 1 cut(s) 1859
Ksp22I TGATCA 1 cut(s) 1981
Kzo9I GATC 8 cut(s) 27, 548, 589, 644, 709, 799, 1567, 1981
LmnI GCTCC 6 cut(s) 97, 105, 588, 691, 1070, 1494
Lsp1109I GCAGC 6 cut(s) 33, 703, 731, 950, 1393, 2005
LweI GCATC 5 cut(s) 242, 352, 975, 1533, 1840
MaeI CTAG 9 cut(s) 419, 468, 615, 668, 692, 852, 1082, 1263, 1649
MaeII ACGT 1 cut(s) 1788
MaeIII GTNAC 5 cut(s) 7, 179, 1448, 1595, 1878
MalI GATC 8 cut(s) 29, 550, 591, 646, 711, 801, 1569, 1983
MboI GATC 8 cut(s) 27, 548, 589, 644, 709, 799, 1567, 1981
MfeI CAATTG 2 cut(s) 12, 1430
MflI RGATCY 4 cut(s) 548, 589, 644, 709
MhlI GDGCHC 4 cut(s) 102, 328, 1441, 1661
MlyI GAGTC 4 cut(s) 218, 1170, 1171, 1936
MmeI TCCRAC 1 cut(s) 1653
Mph1103I ATGCAT 1 cut(s) 1855
MroNI GCCGGC 1 cut(s) 1857
MroXI GAANNNNTTC 3 cut(s) 169, 1295, 1405
MseI TTAA 8 cut(s) 752, 783, 980, 1200, 1257, 1583, 1884, 2054
MslI CAYNNNNRTG 2 cut(s) 348, 1149
MspI CCGG 2 cut(s) 103, 1858
MspR9I CCNGG 5 cut(s) 104, 428, 587, 600, 932
MunI CAATTG 2 cut(s) 12, 1430
Mva1269I GAATGC 1 cut(s) 1025
MvaI CCWGG 4 cut(s) 428, 587, 600, 932
MvnI CGCG 1 cut(s) 1958
MwoI GCNNNNNNNGC 5 cut(s) 255, 323, 1141, 1268, 1999
NaeI GCCGGC 1 cut(s) 1859
NciI CCSGG 1 cut(s) 104
NdeII GATC 8 cut(s) 27, 548, 589, 644, 709, 799, 1567, 1981
NgoMIV GCCGGC 1 cut(s) 1857
NlaIII CATG 9 cut(s) 465, 625, 821, 1639, 1669, 1857, 1988, 2027, 2087
NlaIV GGNNCC 5 cut(s) 327, 591, 604, 711, 1145
NmuCI GTSAC 2 cut(s) 1448, 1878
NsiI ATGCAT 1 cut(s) 1855
NspI RCATGY 2 cut(s) 1639, 1857
PaeI GCATGC 1 cut(s) 1857
PciI ACATGT 1 cut(s) 1635
PctI GAATGC 1 cut(s) 1025
PdiI GCCGGC 1 cut(s) 1859
PdmI GAANNNNTTC 3 cut(s) 169, 1295, 1405
PfeI GAWTC 3 cut(s) 284, 575, 1306
PflMI CCANNNNNTGG 1 cut(s) 1153
PfoI TCCNGGA 1 cut(s) 585
PkrI GCNGC 7 cut(s) 23, 718, 721, 965, 1383, 1960, 1995
PleI GAGTC 4 cut(s) 218, 1170, 1170, 1935
PpsI GAGTC 4 cut(s) 218, 1170, 1170, 1935
PpuMI RGGWCCY 1 cut(s) 602
PscI ACATGT 1 cut(s) 1635
PshAI GACNNNNGTC 2 cut(s) 80, 600
PsiI TTATAA 3 cut(s) 308, 518, 1785
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 602
Psp6I CCWGG 4 cut(s) 426, 585, 598, 930
PspFI CCCAGC 1 cut(s) 892
PspGI CCWGG 4 cut(s) 426, 585, 598, 930
PspN4I GGNNCC 5 cut(s) 327, 591, 604, 711, 1145
PspPI GGNCC 3 cut(s) 353, 602, 2080
PspPPI RGGWCCY 1 cut(s) 602
PstI CTGCAG 1 cut(s) 721
PsuI RGATCY 4 cut(s) 548, 589, 644, 709
RsaI GTAC 6 cut(s) 114, 1172, 1279, 1325, 1331, 1795
RsaNI GTAC 6 cut(s) 113, 1171, 1278, 1324, 1330, 1794
RseI CAYNNNNRTG 2 cut(s) 348, 1149
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 8 cut(s) 752, 783, 980, 1200, 1257, 1583, 1884, 2054
SatI GCNGC 7 cut(s) 22, 717, 720, 964, 1382, 1959, 1994
Sau3AI GATC 8 cut(s) 27, 548, 589, 644, 709, 799, 1567, 1981
Sau96I GGNCC 3 cut(s) 353, 602, 2080
SchI GAGTC 4 cut(s) 218, 1170, 1171, 1936
ScrFI CCNGG 5 cut(s) 104, 428, 587, 600, 932
SduI GDGCHC 4 cut(s) 102, 328, 1441, 1661
SfaNI GCATC 5 cut(s) 242, 352, 975, 1533, 1840
SfcI CTRYAG 3 cut(s) 435, 717, 1095
SinI GGWCC 1 cut(s) 602
SmiMI CAYNNNNRTG 2 cut(s) 348, 1149
SmlI CTYRAG 1 cut(s) 1286
SmoI CTYRAG 1 cut(s) 1286
SphI GCATGC 1 cut(s) 1857
SsiI CCGC 2 cut(s) 221, 1958
SspMI CTAG 9 cut(s) 419, 468, 615, 668, 692, 852, 1082, 1263, 1649
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 5 cut(s) 102, 426, 585, 598, 930
TaaI ACNGT 5 cut(s) 635, 731, 985, 1607, 2064
TaiI ACGT 1 cut(s) 1791
TaqI TCGA 3 cut(s) 726, 1441, 1972
TatI WGTACW 3 cut(s) 1170, 1277, 1793
TauI GCSGC 1 cut(s) 1961
TfiI GAWTC 3 cut(s) 284, 575, 1306
Tru1I TTAA 8 cut(s) 752, 783, 980, 1200, 1257, 1583, 1884, 2054
Tru9I TTAA 8 cut(s) 752, 783, 980, 1200, 1257, 1583, 1884, 2054
TscAI CASTG 3 cut(s) 565, 1164, 1973
TseFI GTSAC 2 cut(s) 1448, 1878
TseI GCWGC 6 cut(s) 21, 716, 719, 963, 1381, 1993
Tsp45I GTSAC 2 cut(s) 1448, 1878
TspDTI ATGAA 4 cut(s) 493, 1518, 1654, 1763
TspRI CASTG 3 cut(s) 565, 1164, 1973
Van91I CCANNNNNTGG 1 cut(s) 1153
VpaK11BI GGWCC 1 cut(s) 602
XagI CCTNNNNNAGG 1 cut(s) 597
XapI RAATTY 4 cut(s) 481, 954, 1724, 2070
XceI RCATGY 2 cut(s) 1639, 1857
XmnI GAANNNNTTC 3 cut(s) 169, 1295, 1405
XspI CTAG 9 cut(s) 419, 468, 615, 668, 692, 852, 1082, 1263, 1649
Zsp2I ATGCAT 1 cut(s) 1855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.