Rw2G036410

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000618
Physical Location & Seq
Reverse (-)
59928108 .. 59936690
8583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G036410.1

Sequence Viewer

Length: 1437 bp
ATGTTAGTTACAATCGAGATTTTTCCAGAAAGTCTGAGAATATTATGGAGCTTGTGGGAAATCCGCGTTACGGTGCTAGTGAGCCTCATCCTACAAGGCATTCTCTTACTGTTAGGCAACAGGAGAAAGCATTCAACCAGAAACTGGCTAAGGCTTATTCTCTGGTTTGCCTACTTGTCCGCAGACTGGGTTGCAACACTCTCACTCAGCCTACTTCCCAGCAATTCGATAGACCATGATGCGAATTCAAAAGACCTAGACTACATCATCATAGCATTTTGGGCTCCATTTCTTCTTCTTCACCTCGGTGGGCCAGACACCATTACCGCGTCATCTTTGGAAGACAATGAGTTGTGGTCGAGGCACTTCCTTGTGCTACTAGTCCAGGTTGCAGTCGCTTTCTACATCTACCTTAGAGCCTGGGGTGTAGTGACAATTTTAGAGAGTCTAGCTATGCTCCCTCCTCCTGATCCAGGCCCCAACTACGCCAGATACACGAATGAGTTCCGCTCTAAGGAATCTGAAGGCTTCAAGGTTGAGTTAGGGAGAGTGATTGCAACTCCTAAAGTCGGTTATTATTCCCATACAGCTCCAAGAAACATTTTTCCGAATGCAGCCAATCTGCAAGATGCCCATCTTTTCTTCAATATCTTCAAAGCGCTCTGTCCTGGTTTATTCTGTCAGGGGTGCCATTTTTCGTGCTGTCAGTTCTGTTTGCATCACTTTTGTTTCTGTCGAAGGCTCATTGAGAAGAAAGCTTACTCTAAAGAAAATGTAATCCTCACTTATGTGTTGTTGGCTGGAGCTATCATCCTAGAGATCTATGCTGTATATTTGTTATTCCTCTCAAATTGGACTATGCTATGGCTAAGTAAGAAAAGGAGATTGAGCAGGGAAATTGTTTTATCCGAACTTAATACGTTTTGCCTTGAAGATACACCTGTGAAGTGCACTATTCTGCAGAAGGTCCAGATCTACAAGAAACTGAAGATATATAAGAACTTGAAAAAGCACCTTTACATCGATTCAAAGGATGTTTATCAGGAGTTGAAAGAATTGATATTTGATCAGCTTCTAAAGAAATCAAGGACTACCCCAGAACTCGAGGATTGCAAGAAATTATGTACTCATAGGGGTGCCTGTATTCTTGATAAAGAATTTGATCAAAGCCTTCTTCTTTGGCATATTGCAACAGACCTCTGTTATTACTCTGATATGAAGAGAACCCCAAACTTTGTCGAGGATCACAGTTGTAAAGATAGCAATTATGTGCCCCTCCATGCTCTACCCAATGGCATCGGACAAATCAGGTTCCAAGACACATGTGCCGAGGCCATTGAATTCTTTGAAGAAAGAAATGCAAAGGAGAACAAAAATGTAACCAAAAAAAAAAGAAATGCAGAGACAAAAAGGCTAGCAAAGCATTACTTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

478

Amino Acids

55.56

Weight (kDa)

8.84

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 143 1.1e-30 Domain of unknown function (DUF4220)
DUF4220 PF13968 232 - 299 5.8e-12 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 687, 1136
AccB7I CCANNNNNTGG 1 cut(s) 144
AccBSI CCGCTC 1 cut(s) 510
AccII CGCG 2 cut(s) 66, 329
AciI CCGC 4 cut(s) 64, 180, 327, 508
AclWI GGATC 2 cut(s) 464, 1251
AcsI RAATTY 3 cut(s) 244, 1157, 1340
AcuI CTGAAG 2 cut(s) 543, 1007
AfaI GTAC 1 cut(s) 1126
AfeI AGCGCT 1 cut(s) 660
AfiI CCNNNNNNNGG 6 cut(s) 70, 144, 186, 473, 514, 569
AflIII ACRYGT 1 cut(s) 1322
AhlI ACTAGT 1 cut(s) 379
AjnI CCWGG 4 cut(s) 384, 419, 472, 667
AleI CACNNNNGTG 2 cut(s) 306, 788
AluBI AGCT 6 cut(s) 51, 452, 590, 758, 806, 1072
AluI AGCT 6 cut(s) 51, 452, 590, 758, 806, 1072
Alw21I GWGCWC 1 cut(s) 953
Alw26I GTCTC 1 cut(s) 1397
Alw44I GTGCAC 1 cut(s) 949
AlwI GGATC 2 cut(s) 464, 1251
AlwNI CAGNNNCTG 1 cut(s) 144
Ama87I CYCGRG 1 cut(s) 1103
Aor51HI AGCGCT 1 cut(s) 660
AoxI GGCC 3 cut(s) 311, 475, 1332
ApaLI GTGCAC 1 cut(s) 949
ApeKI GCWGC 1 cut(s) 614
ApoI RAATTY 3 cut(s) 244, 1157, 1340
Asp700I GAANNNNTTC 2 cut(s) 130, 503
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 3 cut(s) 311, 476, 967
AsuHPI GGTGA 1 cut(s) 293
AsuNHI GCTAGC 1 cut(s) 1414
AvaI CYCGRG 1 cut(s) 1103
AvaII GGWCC 1 cut(s) 967
BaeGI GKGCMC 2 cut(s) 953, 1275
BaeI ACNNNNGTAYC 2 cut(s) 927, 960
BanI GGYRCC 2 cut(s) 687, 1136
BanII GRGCYC 1 cut(s) 286
BarI GAAGNNNNNNTAC 2 cut(s) 743, 775
BbsI GAAGAC 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 953
BbvI GCAGC 1 cut(s) 626
BccI CCATC 1 cut(s) 642
BciT130I CCWGG 4 cut(s) 386, 421, 474, 669
BclI TGATCA 2 cut(s) 1066, 1162
BcoDI GTCTC 1 cut(s) 1397
BcuI ACTAGT 1 cut(s) 379
BfaI CTAG 6 cut(s) 77, 257, 380, 449, 815, 1415
BfmI CTRYAG 1 cut(s) 959
BfoI RGCGCY 1 cut(s) 662
BglII AGATCT 2 cut(s) 819, 972
BisI GCNGC 1 cut(s) 615
BlsI GCNGC 1 cut(s) 616
Bme1390I CCNGG 4 cut(s) 386, 421, 474, 669
Bme18I GGWCC 1 cut(s) 967
BmeT110I CYCGRG 1 cut(s) 1103
BmgT120I GGNCC 3 cut(s) 311, 476, 967
BmiI GGNNCC 5 cut(s) 285, 478, 689, 1138, 1313
BmrFI CCNGG 4 cut(s) 386, 421, 474, 669
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 4 cut(s) 229, 619, 727, 1305
BmtI GCTAGC 1 cut(s) 1418
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 1 cut(s) 348
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 1 cut(s) 822
Bpu10I CCTNAGC 1 cut(s) 149
Bsa29I ATCGAT 1 cut(s) 1023
BsaBI GATNNNNATC 2 cut(s) 633, 1038
BsaJI CCNNGG 3 cut(s) 304, 420, 1329
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 144, 186, 473, 514, 569
Bse1I ACTGG 2 cut(s) 149, 191
Bse8I GATNNNNATC 2 cut(s) 633, 1038
BseBI CCWGG 4 cut(s) 386, 421, 474, 669
BseCI ATCGAT 1 cut(s) 1023
BseDI CCNNGG 3 cut(s) 304, 420, 1329
BseGI GGATG 3 cut(s) 87, 810, 1039
BseJI GATNNNNATC 2 cut(s) 633, 1038
BseLI CCNNNNNNNGG 6 cut(s) 70, 144, 186, 473, 514, 569
BseMII CTCAG 2 cut(s) 26, 220
BseNI ACTGG 2 cut(s) 149, 191
BseRI GAGGAG 1 cut(s) 453
BseSI GKGCMC 2 cut(s) 953, 1275
BseXI GCAGC 1 cut(s) 626
BseYI CCCAGC 1 cut(s) 218
Bsh1236I CGCG 2 cut(s) 66, 329
BshFI GGCC 3 cut(s) 313, 477, 1334
BshNI GGYRCC 2 cut(s) 687, 1136
BshVI ATCGAT 1 cut(s) 1023
BsiHKAI GWGCWC 1 cut(s) 953
BsiHKCI CYCGRG 1 cut(s) 1103
BslI CCNNNNNNNGG 6 cut(s) 70, 144, 186, 473, 514, 569
BsmAI GTCTC 1 cut(s) 1397
BsmI GAATGC 3 cut(s) 99, 130, 616
BsnI GGCC 3 cut(s) 313, 477, 1334
BsoBI CYCGRG 1 cut(s) 1103
Bsp1286I GDGCHC 3 cut(s) 286, 953, 1275
Bsp143I GATC 6 cut(s) 469, 819, 972, 1066, 1162, 1243
BspACI CCGC 4 cut(s) 64, 180, 327, 508
BspANI GGCC 3 cut(s) 313, 477, 1334
BspCNI CTCAG 2 cut(s) 27, 219
BspDI ATCGAT 1 cut(s) 1023
BspFNI CGCG 2 cut(s) 66, 329
BspLI GGNNCC 5 cut(s) 285, 478, 689, 1138, 1313
BspMAI CTGCAG 1 cut(s) 963
BspOI GCTAGC 1 cut(s) 1418
BspPI GGATC 2 cut(s) 464, 1251
BspT107I GGYRCC 2 cut(s) 687, 1136
BsrBI CCGCTC 1 cut(s) 510
BsrI ACTGG 2 cut(s) 149, 191
BssECI CCNNGG 3 cut(s) 304, 420, 1329
BssMI GATC 6 cut(s) 469, 819, 972, 1066, 1162, 1243
Bst2UI CCWGG 4 cut(s) 386, 421, 474, 669
Bst4CI ACNGT 3 cut(s) 73, 111, 1250
Bst6I CTCTTC 1 cut(s) 1214
BstC8I GCNNGC 1 cut(s) 1416
BstDEI CTNAG 6 cut(s) 35, 149, 206, 413, 513, 869
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 3 cut(s) 87, 810, 1039
BstFNI CGCG 2 cut(s) 66, 329
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 6 cut(s) 472, 822, 975, 1069, 1165, 1246
BstMAI GTCTC 1 cut(s) 1397
BstMBI GATC 6 cut(s) 469, 819, 972, 1066, 1162, 1243
BstMWI GCNNNNNNNGC 2 cut(s) 281, 1420
BstNI CCWGG 4 cut(s) 386, 421, 474, 669
BstNSI RCATGY 1 cut(s) 1326
BstSCI CCNGG 4 cut(s) 384, 419, 472, 667
BstSFI CTRYAG 1 cut(s) 959
BstSLI GKGCMC 2 cut(s) 953, 1275
BstUI CGCG 2 cut(s) 66, 329
BstV1I GCAGC 1 cut(s) 626
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 2 cut(s) 819, 972
BstYI RGATCY 2 cut(s) 819, 972
Bsu15I ATCGAT 1 cut(s) 1023
BsuRI GGCC 3 cut(s) 313, 477, 1334
BsuTUI ATCGAT 1 cut(s) 1023
BtsCI GGATG 3 cut(s) 87, 810, 1039
Cac8I GCNNGC 1 cut(s) 1416
CaiI CAGNNNCTG 1 cut(s) 144
CfoI GCGC 1 cut(s) 661
Cfr13I GGNCC 3 cut(s) 311, 476, 967
ClaI ATCGAT 1 cut(s) 1023
CseI GACGC 1 cut(s) 318
Csp6I GTAC 1 cut(s) 1125
CviAII CATG 3 cut(s) 236, 1280, 1323
CviQI GTAC 1 cut(s) 1125
DdeI CTNAG 6 cut(s) 35, 149, 206, 413, 513, 869
DpnI GATC 6 cut(s) 471, 821, 974, 1068, 1164, 1245
DpnII GATC 6 cut(s) 469, 819, 972, 1066, 1162, 1243
Eam1104I CTCTTC 1 cut(s) 1214
EarI CTCTTC 1 cut(s) 1214
Eco24I GRGCYC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 967
Eco47III AGCGCT 1 cut(s) 660
Eco57I CTGAAG 2 cut(s) 543, 1007
Eco88I CYCGRG 1 cut(s) 1103
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 476
EcoRI GAATTC 2 cut(s) 244, 1340
EcoRII CCWGG 4 cut(s) 384, 419, 472, 667
EcoT38I GRGCYC 1 cut(s) 286
FaeI CATG 3 cut(s) 239, 1283, 1326
FalI AAGNNNNNCTT 1 cut(s) 1412
FatI CATG 3 cut(s) 235, 1279, 1322
FbaI TGATCA 2 cut(s) 1066, 1162
Fnu4HI GCNGC 1 cut(s) 615
FokI GGATG 3 cut(s) 74, 797, 1046
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 615
FspBI CTAG 6 cut(s) 77, 257, 380, 449, 815, 1415
GlaI GCGC 1 cut(s) 660
GluI GCNGC 1 cut(s) 615
GsaI CCCAGC 1 cut(s) 222
GsuI CTGGAG 1 cut(s) 822
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 3 cut(s) 313, 477, 1334
HgaI GACGC 1 cut(s) 318
HhaI GCGC 1 cut(s) 661
Hin1II CATG 3 cut(s) 239, 1283, 1326
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HindIII AAGCTT 1 cut(s) 756
HinfI GANTC 3 cut(s) 445, 518, 1025
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 1 cut(s) 951
Hpy188I TCNGA 6 cut(s) 36, 523, 609, 910, 1213, 1301
Hpy188III TCNNGA 6 cut(s) 16, 26, 467, 970, 1043, 1148
Hpy8I GTNNAC 1 cut(s) 951
HpyAV CCTTC 4 cut(s) 518, 732, 958, 1181
HpyCH4III ACNGT 3 cut(s) 73, 111, 1250
HpyCH4IV ACGT 1 cut(s) 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 1420
HpyF3I CTNAG 6 cut(s) 35, 149, 206, 413, 513, 869
HpySE526I ACGT 1 cut(s) 920
Hsp92II CATG 3 cut(s) 239, 1283, 1326
HspAI GCGC 1 cut(s) 659
Ksp22I TGATCA 2 cut(s) 1066, 1162
Kzo9I GATC 6 cut(s) 469, 819, 972, 1066, 1162, 1243
LmnI GCTCC 5 cut(s) 48, 289, 462, 595, 803
Lsp1109I GCAGC 1 cut(s) 626
LweI GCATC 4 cut(s) 229, 619, 727, 1305
MaeI CTAG 6 cut(s) 77, 257, 380, 449, 815, 1415
MaeII ACGT 1 cut(s) 920
MaeIII GTNAC 4 cut(s) 7, 67, 430, 1378
MalI GATC 6 cut(s) 471, 821, 974, 1068, 1164, 1245
MbiI CCGCTC 1 cut(s) 510
MboI GATC 6 cut(s) 469, 819, 972, 1066, 1162, 1243
MflI RGATCY 2 cut(s) 819, 972
MhlI GDGCHC 3 cut(s) 286, 953, 1275
MlyI GAGTC 1 cut(s) 454
MroXI GAANNNNTTC 2 cut(s) 130, 503
MseI TTAA 1 cut(s) 915
MslI CAYNNNNRTG 3 cut(s) 306, 788, 1134
MspR9I CCNGG 4 cut(s) 386, 421, 474, 669
Mva1269I GAATGC 3 cut(s) 99, 130, 616
MvaI CCWGG 4 cut(s) 386, 421, 474, 669
MvnI CGCG 2 cut(s) 66, 329
MwoI GCNNNNNNNGC 2 cut(s) 281, 1420
NdeII GATC 6 cut(s) 469, 819, 972, 1066, 1162, 1243
NheI GCTAGC 1 cut(s) 1414
NlaIII CATG 3 cut(s) 239, 1283, 1326
NlaIV GGNNCC 5 cut(s) 285, 478, 689, 1138, 1313
NmeAIII GCCGAG 1 cut(s) 1354
NmuCI GTSAC 1 cut(s) 430
NspI RCATGY 1 cut(s) 1326
OliI CACNNNNGTG 2 cut(s) 306, 788
PaeR7I CTCGAG 1 cut(s) 1103
PciI ACATGT 1 cut(s) 1322
PctI GAATGC 3 cut(s) 99, 130, 616
PdmI GAANNNNTTC 2 cut(s) 130, 503
PfeI GAWTC 2 cut(s) 518, 1025
PflMI CCANNNNNTGG 1 cut(s) 144
PkrI GCNGC 1 cut(s) 616
PleI GAGTC 1 cut(s) 453
PpsI GAGTC 1 cut(s) 453
PscI ACATGT 1 cut(s) 1322
Psp6I CCWGG 4 cut(s) 384, 419, 472, 667
PspFI CCCAGC 1 cut(s) 218
PspGI CCWGG 4 cut(s) 384, 419, 472, 667
PspN4I GGNNCC 5 cut(s) 285, 478, 689, 1138, 1313
PspPI GGNCC 3 cut(s) 311, 476, 967
PspXI VCTCGAGB 1 cut(s) 1103
PstI CTGCAG 1 cut(s) 963
PstNI CAGNNNCTG 1 cut(s) 144
PsuI RGATCY 2 cut(s) 819, 972
RsaI GTAC 1 cut(s) 1126
RsaNI GTAC 1 cut(s) 1125
RseI CAYNNNNRTG 3 cut(s) 306, 788, 1134
SaqAI TTAA 1 cut(s) 915
SatI GCNGC 1 cut(s) 615
Sau3AI GATC 6 cut(s) 469, 819, 972, 1066, 1162, 1243
Sau96I GGNCC 3 cut(s) 311, 476, 967
SchI GAGTC 1 cut(s) 454
ScrFI CCNGG 4 cut(s) 386, 421, 474, 669
SduI GDGCHC 3 cut(s) 286, 953, 1275
SfaNI GCATC 4 cut(s) 229, 619, 727, 1305
SfcI CTRYAG 1 cut(s) 959
Sfr274I CTCGAG 1 cut(s) 1103
SinI GGWCC 1 cut(s) 967
SlaI CTCGAG 1 cut(s) 1103
SmiMI CAYNNNNRTG 3 cut(s) 306, 788, 1134
SmlI CTYRAG 2 cut(s) 1103, 1429
SmoI CTYRAG 2 cut(s) 1103, 1429
SpeI ACTAGT 1 cut(s) 379
SsiI CCGC 4 cut(s) 64, 180, 327, 508
SspI AATATT 1 cut(s) 42
SspMI CTAG 6 cut(s) 77, 257, 380, 449, 815, 1415
StyD4I CCNGG 4 cut(s) 384, 419, 472, 667
TaaI ACNGT 3 cut(s) 73, 111, 1250
TaiI ACGT 1 cut(s) 923
TaqI TCGA 7 cut(s) 15, 227, 359, 736, 1023, 1104, 1239
TatI WGTACW 1 cut(s) 1124
TfiI GAWTC 2 cut(s) 518, 1025
Tru1I TTAA 1 cut(s) 915
Tru9I TTAA 1 cut(s) 915
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 1 cut(s) 614
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 1 cut(s) 1232
Van91I CCANNNNNTGG 1 cut(s) 144
VneI GTGCAC 1 cut(s) 949
VpaK11BI GGWCC 1 cut(s) 967
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 3 cut(s) 244, 1157, 1340
XceI RCATGY 1 cut(s) 1326
XhoI CTCGAG 1 cut(s) 1103
XmnI GAANNNNTTC 2 cut(s) 130, 503
XspI CTAG 6 cut(s) 77, 257, 380, 449, 815, 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.