Prupe.8G014800_v2.0.a1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000618
Physical Location & Seq
Forward (+)
1235782 .. 1236243
462 bp
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UTR
Exon/CDS
Intron
Prupe.8G014800.1

Sequence Viewer

Length: 462 bp
ATGTGCCCCTTCATGCTACTGAATGGGATTGGACAGATAAGGTTTAGAGACACATGTGCTGAGGCTGAAGAATTGTTCAAGGAAAAGAAAATAATGAAATTGGGTGAAAGGAAAGCATGCACAAAGCTACTGGATGTGTGCACAGACATCCTTCCATCTAAAGTGAAAGGAGATAGAAGCAAGTCAGTTCTATTTGATGCATGCAGGCTTGCTAAAGCCTTGCAGTCCCTGGAGTCAGATAGAGGGTGGACTAATGGGAGAAAGTGGCAGTTTGTAAGTCATGTGTGGGTGGAGATGCTCCCTTATGCCGCCAATCAATGTCGATGGAGTGGTCAGGCTCAGCAGCTCAGGCGAGGAGGAGAGCTGCTCACCCATGTCTGGCTTCTTATGGCACATCTTGGTTTAACTGAACAGTTCCAAATTTCAGAGGGCCATGCAAGGGCAAAACTGATTGTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

154

Amino Acids

17.46

Weight (kDa)

9.28

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 309
AcsI RAATTY 1 cut(s) 420
AcuI CTGAAG 1 cut(s) 87
AfiI CCNNNNNNNGG 2 cut(s) 378, 439
AflIII ACRYGT 1 cut(s) 53
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 3 cut(s) 127, 346, 364
AluI AGCT 3 cut(s) 127, 346, 364
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 1 cut(s) 42
Alw44I GTGCAC 1 cut(s) 139
AlwNI CAGNNNCTG 1 cut(s) 229
AoxI GGCC 1 cut(s) 430
ApaLI GTGCAC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 343, 364
ApoI RAATTY 1 cut(s) 420
AspS9I GGNCC 1 cut(s) 430
AsuHPI GGTGA 2 cut(s) 116, 361
BaeGI GKGCMC 2 cut(s) 8, 143
Bbv12I GWGCWC 1 cut(s) 143
BbvCI CCTCAGC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 351, 355
BccI CCATC 2 cut(s) 163, 318
BciT130I CCWGG 1 cut(s) 230
BcoDI GTCTC 1 cut(s) 42
BisI GCNGC 3 cut(s) 309, 344, 365
BlpI GCTNAGC 1 cut(s) 339
BlsI GCNGC 3 cut(s) 310, 345, 366
Bme1390I CCNGG 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 430
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 2 cut(s) 187, 285
BplI GAGNNNNNCTC 2 cut(s) 351, 383
BpmI CTGGAG 1 cut(s) 251
Bpu10I CCTNAGC 2 cut(s) 60, 347
Bpu1102I GCTNAGC 1 cut(s) 339
BsaJI CCNNGG 1 cut(s) 228
Bsc4I CCNNNNNNNGG 2 cut(s) 378, 439
Bse1I ACTGG 1 cut(s) 135
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 2 cut(s) 139, 147
BseLI CCNNNNNNNGG 2 cut(s) 378, 439
BseMII CTCAG 3 cut(s) 51, 353, 361
BseNI ACTGG 1 cut(s) 135
BseRI GAGGAG 2 cut(s) 369, 372
BseSI GKGCMC 2 cut(s) 8, 143
BseXI GCAGC 2 cut(s) 351, 355
BshFI GGCC 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 143
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 2 cut(s) 378, 439
BsmAI GTCTC 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 211
BsnI GGCC 1 cut(s) 432
Bsp1286I GDGCHC 2 cut(s) 8, 143
Bsp1720I GCTNAGC 1 cut(s) 339
BspACI CCGC 1 cut(s) 309
BspANI GGCC 1 cut(s) 432
BspCNI CTCAG 3 cut(s) 52, 352, 360
BsrI ACTGG 1 cut(s) 135
BssECI CCNNGG 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 414
BstC8I GCNNGC 4 cut(s) 118, 202, 206, 210
BstDEI CTNAG 3 cut(s) 60, 339, 347
BstF5I GGATG 2 cut(s) 139, 147
BstMAI GTCTC 1 cut(s) 42
BstMWI GCNNNNNNNGC 1 cut(s) 349
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 3 cut(s) 57, 120, 204
BstSCI CCNGG 1 cut(s) 228
BstSLI GKGCMC 2 cut(s) 8, 143
BstV1I GCAGC 2 cut(s) 351, 355
BsuRI GGCC 1 cut(s) 432
BtsCI GGATG 2 cut(s) 139, 147
Cac8I GCNNGC 4 cut(s) 118, 202, 206, 210
CaiI CAGNNNCTG 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 430
CviAII CATG 7 cut(s) 13, 54, 117, 201, 281, 374, 434
CviJI RGCY 9 cut(s) 65, 127, 208, 218, 338, 346, 364, 382, 432
CviKI_1 RGCY 9 cut(s) 65, 127, 208, 218, 338, 346, 364, 382, 432
DdeI CTNAG 3 cut(s) 60, 339, 347
Eco57I CTGAAG 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 228
EcoT22I ATGCAT 1 cut(s) 202
FaeI CATG 7 cut(s) 16, 57, 120, 204, 284, 377, 437
FaiI YATR 9 cut(s) 14, 55, 118, 202, 282, 306, 375, 389, 435
FaqI GGGAC 1 cut(s) 211
FatI CATG 7 cut(s) 12, 53, 116, 200, 280, 373, 433
Fnu4HI GCNGC 3 cut(s) 309, 344, 365
FokI GGATG 2 cut(s) 134, 146
Fsp4HI GCNGC 3 cut(s) 309, 344, 365
GluI GCNGC 3 cut(s) 309, 344, 365
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 1 cut(s) 432
Hin1II CATG 7 cut(s) 16, 57, 120, 204, 284, 377, 437
HinfI GANTC 1 cut(s) 233
HphI GGTGA 2 cut(s) 116, 361
Hpy166II GTNNAC 2 cut(s) 141, 249
Hpy188I TCNGA 2 cut(s) 238, 427
Hpy8I GTNNAC 2 cut(s) 141, 249
HpyAV CCTTC 2 cut(s) 19, 161
HpyCH4III ACNGT 1 cut(s) 414
HpyCH4V TGCA 6 cut(s) 120, 141, 200, 204, 223, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 349
HpyF3I CTNAG 3 cut(s) 60, 339, 347
Hsp92II CATG 7 cut(s) 16, 57, 120, 204, 284, 377, 437
LmnI GCTCC 1 cut(s) 303
LpnPI CCDG 7 cut(s) 116, 190, 215, 242, 320, 334, 364
Lsp1109I GCAGC 2 cut(s) 351, 355
LweI GCATC 2 cut(s) 187, 285
MboII GAAGA 1 cut(s) 80
MhlI GDGCHC 2 cut(s) 8, 143
MluCI AATT 3 cut(s) 71, 98, 420
MlyI GAGTC 1 cut(s) 242
MnlI CCTC 5 cut(s) 55, 236, 347, 350, 421
Mph1103I ATGCAT 1 cut(s) 202
MseI TTAA 2 cut(s) 404, 460
MspR9I CCNGG 1 cut(s) 230
MvaI CCWGG 1 cut(s) 230
MwoI GCNNNNNNNGC 1 cut(s) 349
NlaIII CATG 7 cut(s) 16, 57, 120, 204, 284, 377, 437
NsiI ATGCAT 1 cut(s) 202
NspI RCATGY 3 cut(s) 57, 120, 204
PaeI GCATGC 2 cut(s) 120, 204
PciI ACATGT 1 cut(s) 53
PkrI GCNGC 3 cut(s) 310, 345, 366
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PscI ACATGT 1 cut(s) 53
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspPI GGNCC 1 cut(s) 430
PstNI CAGNNNCTG 1 cut(s) 229
SaqAI TTAA 2 cut(s) 404, 460
SatI GCNGC 3 cut(s) 309, 344, 365
Sau96I GGNCC 1 cut(s) 430
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 2 cut(s) 8, 143
SetI ASST 4 cut(s) 44, 129, 348, 366
SfaNI GCATC 2 cut(s) 187, 285
SphI GCATGC 2 cut(s) 120, 204
Sse9I AATT 3 cut(s) 71, 98, 420
SsiI CCGC 1 cut(s) 309
StyD4I CCNGG 1 cut(s) 228
TaaI ACNGT 1 cut(s) 414
TaqI TCGA 1 cut(s) 322
TasI AATT 3 cut(s) 71, 98, 420
TauI GCSGC 1 cut(s) 311
Tru1I TTAA 2 cut(s) 404, 460
Tru9I TTAA 2 cut(s) 404, 460
TseI GCWGC 2 cut(s) 343, 364
TspDTI ATGAA 1 cut(s) 110
VneI GTGCAC 1 cut(s) 139
XapI RAATTY 1 cut(s) 420
XceI RCATGY 3 cut(s) 57, 120, 204
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.