RchiOBHm_Chr5g0066471

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000618
Physical Location & Seq
Reverse (-)
72640153 .. 72640677
525 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34224

Sequence Viewer

Length: 525 bp
ATGATGTATATTCTTGCCATCTGCCCTTTCATGTTACCCCAAGGGATTGGACAGATTTTGTTTCGACAAATATGTGAGTCTGCCACAACATTTTTTTATGAGAGAAGGGAAACCATAAGTAAGACAGAGCGATTGGTTGGAAAAAGTGCCAGTCAGGTGTTGTACGAGATGAAGTCAGAGTTGAATCCATGGCTAGATGAGTTTGAGTCAGGAGATGGAGGAAAACTAAACAATGTAGTGTTGCTTGAAGGGTGTGTGCTTGCACATAAGTTGCAGGCCCTGGAAACGGAGGAGAGCTGGACTAATGAAGATAAATGGGGAATGATAAGTCGAGTGTGGATAGAAATGTTGTGTTTTGCAGCAAGCCAATGTGGATGGACGGAGCACGGTCAACAACTTAGGCGTGGAGGAGAGCTTCTTACTCATGTCTCCCTTCTCATGGCAAATCTTGGTATCGGTGAACAGAGTAAGATATTGAGTGAGCAGGACATGGACCGGTTACAGGATTTAAAAAAACTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

174

Amino Acids

19.85

Weight (kDa)

5.03

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF594 PF04578 100 - 152 1.8e-21 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 3 cut(s) 286, 439, 502
AgeI ACCGGT 1 cut(s) 495
AgsI TTSAA 2 cut(s) 184, 248
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 2 cut(s) 297, 415
AluI AGCT 2 cut(s) 297, 415
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 433
AlwNI CAGNNNCTG 1 cut(s) 280
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 1 cut(s) 359
AsiGI ACCGGT 1 cut(s) 495
AspS9I GGNCC 2 cut(s) 277, 493
AsuHPI GGTGA 1 cut(s) 470
AvaII GGWCC 1 cut(s) 493
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 371
BccI CCATC 3 cut(s) 26, 209, 369
BciT130I CCWGG 1 cut(s) 281
BcoDI GTCTC 1 cut(s) 433
BfaI CTAG 1 cut(s) 194
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 493
BmgT120I GGNCC 2 cut(s) 277, 493
BmrFI CCNGG 1 cut(s) 281
BsaJI CCNNGG 3 cut(s) 40, 188, 279
BsaWI WCCGGW 1 cut(s) 495
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 439, 502
Bse118I RCCGGY 1 cut(s) 495
Bse1I ACTGG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 3 cut(s) 40, 188, 279
BseGI GGATG 1 cut(s) 380
BseLI CCNNNNNNNGG 3 cut(s) 286, 439, 502
BseNI ACTGG 1 cut(s) 150
BseRI GAGGAG 2 cut(s) 305, 423
BseXI GCAGC 1 cut(s) 371
BshFI GGCC 1 cut(s) 278
BshTI ACCGGT 1 cut(s) 495
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 1 cut(s) 496
BslI CCNNNNNNNGG 3 cut(s) 286, 439, 502
BsmAI GTCTC 1 cut(s) 433
BsnI GGCC 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 387
Bsp19I CCATGG 1 cut(s) 188
BspANI GGCC 1 cut(s) 278
BsrFI RCCGGY 1 cut(s) 495
BsrI ACTGG 1 cut(s) 150
BssAI RCCGGY 1 cut(s) 495
BssECI CCNNGG 3 cut(s) 40, 188, 279
BssT1I CCWWGG 2 cut(s) 40, 188
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 389
BstC8I GCNNGC 3 cut(s) 261, 276, 364
BstDEI CTNAG 1 cut(s) 398
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 380
BstMAI GTCTC 1 cut(s) 433
BstNI CCWGG 1 cut(s) 281
BstSCI CCNGG 1 cut(s) 279
BstV1I GCAGC 1 cut(s) 371
BstXI CCANNNNNNTGG 1 cut(s) 47
BsuRI GGCC 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 1 cut(s) 380
Cac8I GCNNGC 3 cut(s) 261, 276, 364
CaiI CAGNNNCTG 1 cut(s) 280
Cfr10I RCCGGY 1 cut(s) 495
Cfr13I GGNCC 2 cut(s) 277, 493
Csp6I GTAC 1 cut(s) 163
CspAI ACCGGT 1 cut(s) 495
CviAII CATG 5 cut(s) 31, 189, 425, 439, 490
CviJI RGCY 5 cut(s) 193, 278, 297, 366, 415
CviKI_1 RGCY 5 cut(s) 193, 278, 297, 366, 415
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 1 cut(s) 398
DraI TTTAAA 1 cut(s) 510
Eco130I CCWWGG 2 cut(s) 40, 188
Eco47I GGWCC 1 cut(s) 493
EcoO109I RGGNCCY 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 2 cut(s) 40, 188
ErhI CCWWGG 2 cut(s) 40, 188
FaeI CATG 5 cut(s) 34, 192, 428, 442, 493
FatI CATG 5 cut(s) 30, 188, 424, 438, 489
Fnu4HI GCNGC 1 cut(s) 360
FokI GGATG 1 cut(s) 387
Fsp4HI GCNGC 1 cut(s) 360
FspBI CTAG 1 cut(s) 194
GluI GCNGC 1 cut(s) 360
HaeIII GGCC 1 cut(s) 278
HapII CCGG 1 cut(s) 496
Hin1II CATG 5 cut(s) 34, 192, 428, 442, 493
HincII GTYRAC 1 cut(s) 392
HindII GTYRAC 1 cut(s) 392
HinfI GANTC 3 cut(s) 77, 184, 206
HpaII CCGG 1 cut(s) 496
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 2 cut(s) 392, 461
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 1 cut(s) 210
Hpy8I GTNNAC 2 cut(s) 392, 461
HpyAV CCTTC 3 cut(s) 99, 242, 443
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4V TGCA 3 cut(s) 263, 274, 359
HpyF3I CTNAG 1 cut(s) 398
Hsp92II CATG 5 cut(s) 34, 192, 428, 442, 493
LmnI GCTCC 1 cut(s) 382
Lsp1109I GCAGC 1 cut(s) 371
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 2 cut(s) 33, 498
MboII GAAGA 1 cut(s) 320
MhlI GDGCHC 1 cut(s) 387
MlyI GAGTC 2 cut(s) 86, 215
MmeI TCCRAC 1 cut(s) 118
MnlI CCTC 3 cut(s) 212, 283, 401
MseI TTAA 1 cut(s) 509
MspI CCGG 1 cut(s) 496
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
NcoI CCATGG 1 cut(s) 188
NlaIII CATG 5 cut(s) 34, 192, 428, 442, 493
PfeI GAWTC 1 cut(s) 184
PinAI ACCGGT 1 cut(s) 495
PkrI GCNGC 1 cut(s) 361
PleI GAGTC 2 cut(s) 85, 214
PpsI GAGTC 2 cut(s) 85, 214
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspPI GGNCC 2 cut(s) 277, 493
PstNI CAGNNNCTG 1 cut(s) 280
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
SaqAI TTAA 1 cut(s) 509
SatI GCNGC 1 cut(s) 360
Sau96I GGNCC 2 cut(s) 277, 493
SchI GAGTC 2 cut(s) 86, 215
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 387
SetI ASST 3 cut(s) 159, 299, 417
SinI GGWCC 1 cut(s) 493
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 2 cut(s) 40, 188
TaaI ACNGT 1 cut(s) 389
TaqI TCGA 2 cut(s) 64, 331
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 1 cut(s) 509
Tru9I TTAA 1 cut(s) 509
TseI GCWGC 1 cut(s) 359
TspDTI ATGAA 3 cut(s) 19, 185, 321
TspGWI ACGGA 2 cut(s) 302, 395
VpaK11BI GGWCC 1 cut(s) 493
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.