Rh2BG457100

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000618
Physical Location & Seq
Reverse (-)
64724933 .. 64725334
402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG457100.1

Sequence Viewer

Length: 402 bp
ATGAAGAGAACCCCAAACTTTGTCGAGGTTCACAGTTGTAAAGATAGCAAGTCTATATCGGAATATATGCTGTATCTTCTGGTTATGTGCCCCTCCATGCTCTACCCAATGGCATCGGACAAATCAGGTTCCAAGACACATGTGCCGAGGCCATTAAATTCTTTGAAGAAAGAAAATGCAAAGGAGAACAAAAATGTAACCAAAAAAAGAAAATGCAGAGACAAAAAGGCTAGCAAAGCATTACTTGAGGTGAGCACTGAAATACCTCATGCTGAAATTAAAGGAGATAGAAGCAAGCCTGTGCTATTTGATGCCTGTAGGCTAGCGAAGACATTGCAATTGCTGGAAACTGAAGAAAAATGGGAGAATAAACAGAAGTGGAAGTTGATAAGTCATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

133

Amino Acids

15.34

Weight (kDa)

9.65

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 157
AcuI CTGAAG 1 cut(s) 372
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 1 cut(s) 166
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 1 cut(s) 213
AoxI GGCC 1 cut(s) 149
ApoI RAATTY 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 262
AsuNHI GCTAGC 2 cut(s) 230, 322
BaeGI GKGCMC 1 cut(s) 92
BbsI GAAGAC 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 257
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BcoDI GTCTC 1 cut(s) 213
BfaI CTAG 2 cut(s) 231, 323
BfmI CTRYAG 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 130
BmsI GCATC 2 cut(s) 122, 301
BmtI GCTAGC 2 cut(s) 234, 326
BpiI GAAGAC 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 332
BseDI CCNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 332
BseSI GKGCMC 1 cut(s) 92
BshFI GGCC 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 257
BsmAI GTCTC 1 cut(s) 213
BsnI GGCC 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 92, 257
BspANI GGCC 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 130
BspOI GCTAGC 2 cut(s) 234, 326
BsrDI GCAATG 1 cut(s) 332
BssECI CCNNGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 35
BstC8I GCNNGC 3 cut(s) 232, 296, 324
BstMAI GTCTC 1 cut(s) 213
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNSI RCATGY 1 cut(s) 143
BstSFI CTRYAG 1 cut(s) 316
BstSLI GKGCMC 1 cut(s) 92
BstV2I GAAGAC 1 cut(s) 335
BsuRI GGCC 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 3 cut(s) 232, 296, 324
CviAII CATG 4 cut(s) 97, 140, 269, 395
CviJI RGCY 4 cut(s) 151, 230, 298, 322
CviKI_1 RGCY 4 cut(s) 151, 230, 298, 322
Eco57I CTGAAG 1 cut(s) 372
FaeI CATG 4 cut(s) 100, 143, 272, 398
FaiI YATR 8 cut(s) 56, 66, 68, 86, 98, 141, 270, 396
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FatI CATG 4 cut(s) 96, 139, 268, 394
FspBI CTAG 2 cut(s) 231, 323
HaeIII GGCC 1 cut(s) 151
Hin1II CATG 4 cut(s) 100, 143, 272, 398
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 2 cut(s) 61, 118
Hpy8I GTNNAC 1 cut(s) 31
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4V TGCA 3 cut(s) 179, 216, 337
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
Hsp92II CATG 4 cut(s) 100, 143, 272, 398
LpnPI CCDG 5 cut(s) 65, 111, 312, 328, 329
LweI GCATC 2 cut(s) 122, 301
MaeI CTAG 2 cut(s) 231, 323
MaeIII GTNAC 1 cut(s) 196
MboII GAAGA 5 cut(s) 16, 68, 178, 340, 365
MfeI CAATTG 1 cut(s) 338
MhlI GDGCHC 2 cut(s) 92, 257
MluCI AATT 3 cut(s) 157, 276, 338
MnlI CCTC 5 cut(s) 19, 103, 141, 241, 276
MseI TTAA 2 cut(s) 155, 279
MunI CAATTG 1 cut(s) 338
MwoI GCNNNNNNNGC 1 cut(s) 236
NheI GCTAGC 2 cut(s) 230, 322
NlaIII CATG 4 cut(s) 100, 143, 272, 398
NlaIV GGNNCC 1 cut(s) 130
NmeAIII GCCGAG 1 cut(s) 171
NspI RCATGY 1 cut(s) 143
PciI ACATGT 1 cut(s) 139
PscI ACATGT 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 130
SaqAI TTAA 2 cut(s) 155, 279
SduI GDGCHC 2 cut(s) 92, 257
SetI ASST 4 cut(s) 30, 130, 252, 268
SfaNI GCATC 2 cut(s) 122, 301
SfcI CTRYAG 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 3 cut(s) 157, 276, 338
SspMI CTAG 2 cut(s) 231, 323
TaaI ACNGT 1 cut(s) 35
TaqI TCGA 1 cut(s) 24
TasI AATT 3 cut(s) 157, 276, 338
Tru1I TTAA 2 cut(s) 155, 279
Tru9I TTAA 2 cut(s) 155, 279
TscAI CASTG 1 cut(s) 262
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 262
XapI RAATTY 1 cut(s) 157
XceI RCATGY 1 cut(s) 143
XspI CTAG 2 cut(s) 231, 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.