Prupe.8G037600_v2.0.a1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000618
Physical Location & Seq
Reverse (-)
3496051 .. 3498628
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G037600.1

Sequence Viewer

Length: 2172 bp
ATGCGGATTTTCTCAGAAAGACTACGCATACTATGGAGTTTGTGGGAAATCCGTGTTATGGTACCATTAAGCCTCATCTTACAGGCCATTCTCTTGCTCATCGGCAACAGAAGAAAGTATTCAACCAGAAACTGGCTCAGATTTCTGTGGCTTGCCTACTTGTCAGCAGATTGGGTTGCAACCGTCTCTCTCAGCCTACTTTCCAGCAACTCATCAAACGACCATGCAACTCATTCTCCAGACCCCAATCAGATCGTCACGGCATTCTGGGCTCCATTTCTTTTGCTTCATCTGGCTGGCCCTGACACCATTACTGCATACTCATTGGAAGACAATGAGCTGTGGTGGAGGCACTTGCTTGTTCTCCTTGTCCAGGTGTCTCTGGCTTTCTATATCTTTCTTCAAGCCTGGGTAGGTCATACCCTTAACTTGTTAGCCATTCCAATTTTTATTGCTGGCGCTATCAAGTTTGGAGAAAGGACTTGGGTTTTGAGGTCTGCAAGCAGCAAGCATTTTAGAGAGGCTATGTTCCCTCCTCCTGATCCGGGCCCCAACTACGCGAGATTCACGGACGAGTTCTGCTCAAACCAGAAAGAAGGGTACGTGGTTAAGTTAGGGGAAGAAACTGAAGCTCCTACAACAGGTGATTATTCCTCCTTTACAGCTCCTAACATCTTTCCCAATGCAGCCAATTTGCATCATGCCTCTGTTTTCTTAAAAGTTTTCAAGCGCCTTTTCGCTGATCTCATCCTCAGCATCCATGACATCTTGAACAGCCGGTCCTTCTTCCTGAACAGATCCTGCAACGAAGCTTTCCAAGTAATTGAGATTGAGCTCGGGTTTATGTATGATTTGTTCTATACAAAGGCGGTCCTGGTTTACTCTGTGCAAGGTGTCATTTTTCGTCTCAGCAGTTTTGTTTGCATAGTTTCTGTTTCTGCAGCGTTCTTGGTCATCGACAAGAAAGATTTCTACAAAGTAAATGTAACCATCACTTACATGTTGCTGGCTGGAGCAATCATCCTAGAGGTCTGTGCTGTAATTTTATCACTCTCATCAGACTGGACCAAACTTTGGCTGAATAAGAAGCACAACAAGGTAGTGGAATATTTGTTGCATCCGATCACTTCATCAATTCCATTAGCTGAAACCAAAAGGTGGTCTAATGTCATTTTACAGTTCAACTTAATAAGTTTTTGCTGTGAAGATGACCCGGCACATTGCAGCTTTCTCCACAACTACAAGATCCCATGGATATACAAAAAATTCAAGAAGTCTCTCTATAAGAATTCGAAGGAAGTCCCTATAGAGTTGAAAGAATTGATATTTCAGCAGCTTATAAAGAAATCGAGGAGTGCTCCAAAAGCCGAGTCTTGGAAGAAATTTTGTACTTGCAAGGGGGAGTGGGTTCTTGAAACTGACAAGTGTCTTGACAAACTTGGTTGGAGCATCCAAGACGAATTTGATCAGAGCATTCTTCTCTGGCATATTGCAACGGACCTCTGTTACTACTCTGATCTAGAAGACAACCCAAATTCTGATCTGGCCAAGAAACCAAATTCCCTCAGCAGTCGAAGTTGGAAAGCCAGCAAGTTCTTATCAGAGTATTTGTTGTATCTCCTAGTCATGCGGCCGTTCATGCTGCCCAATGGGATCGGACAAATCAGATTCCAGGACACCTGCGCCGAAGCGGATGAATTCTTTAAACAAAGAAAATGCAAAGGAGATAGGAAGGCTTGCAAAGCATCGCCTGAGGTGAGCACCGGAGTTCCAGCTGCTGAATCCTCCGAACAAAGAAAACCCAAAGAAGACCGACAAAAGAAGGCTTGCACAGCATTACTTGGGGTGAGCACCGAAGTTAATCCAGCTGAAGTCAAAGGGGATAGAAGCAAGTCAGTGCTGTTTGATGCATGCCGACTTACTAAAGATTTGCAATCGCTGGAAACAGAGAAACATTGGGAGAATCAGAAGAAGTGGGAGTTGATAAGTCATGTGTGGGTGGAAATGCTATCTTACGCTGCCTGTCAATGTCAATGGAACCACCACGCCCGGCAACTCAGGCGAGGTGGTGAGCTGCTCACTCATGTCTGGCTTCTTATGGCGCATCTTGGTATAACTGAACAGTTTCAAATTTCAAGGGGCTATGCCAGAGCTAGTTTGATTGTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

724

Amino Acids

83.14

Weight (kDa)

8.73

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1340
AarI CACCTGC 1 cut(s) 1688
Acc36I ACCTGC 1 cut(s) 1688
Acc65I GGTACC 1 cut(s) 61
AccB1I GGYRCC 1 cut(s) 61
AccB7I CCANNNNNTGG 3 cut(s) 132, 1074, 1158
AccII CGCG 1 cut(s) 560
AciI CCGC 4 cut(s) 4, 869, 1630, 1691
AclWI GGATC 4 cut(s) 536, 792, 1240, 1661
AcoI YGGCCR 2 cut(s) 1545, 1631
AcsI RAATTY 8 cut(s) 1265, 1288, 1382, 1460, 1534, 1558, 1697, 2130
AcuI CTGAAG 2 cut(s) 648, 1890
AfaI GTAC 3 cut(s) 63, 602, 1390
AfiI CCNNNNNNNGG 8 cut(s) 58, 132, 373, 545, 641, 1074, 1158, 1374
AflIII ACRYGT 1 cut(s) 999
AjnI CCWGG 4 cut(s) 372, 407, 873, 1671
AloI GAACNNNNNNTCC 2 cut(s) 764, 796
Alw21I GWGCWC 4 cut(s) 837, 1360, 1763, 1853
Alw26I GTCTC 4 cut(s) 190, 384, 911, 1281
AlwI GGATC 4 cut(s) 536, 792, 1240, 1661
AlwNI CAGNNNCTG 3 cut(s) 132, 801, 1778
Ama87I CYCGRG 1 cut(s) 836
AoxI GGCC 5 cut(s) 84, 298, 547, 1545, 1631
ApaI GGGCCC 1 cut(s) 551
ApeKI GCWGC 9 cut(s) 504, 686, 941, 1224, 1333, 1642, 1775, 2018, 2074
ApoI RAATTY 8 cut(s) 1265, 1288, 1382, 1460, 1534, 1558, 1697, 2130
ArsI GACNNNNNNTTYG 4 cut(s) 472, 504, 1355, 1387
Asp700I GAANNNNTTC 3 cut(s) 118, 968, 2124
Asp718I GGTACC 1 cut(s) 61
AspLEI GCGC 4 cut(s) 461, 732, 1685, 2104
AspS9I GGNCC 7 cut(s) 299, 547, 548, 780, 871, 1065, 1498
AsuC2I CCSGG 3 cut(s) 546, 1214, 2050
AsuHPI GGTGA 4 cut(s) 656, 1768, 1858, 2081
AsuII TTCGAA 1 cut(s) 1292
AvaI CYCGRG 1 cut(s) 836
AvaII GGWCC 4 cut(s) 780, 871, 1065, 1498
AxyI CCTNAGG 1 cut(s) 1752
BaeGI GKGCMC 1 cut(s) 551
BalI TGGCCA 1 cut(s) 1547
BanI GGYRCC 1 cut(s) 61
BanII GRGCYC 3 cut(s) 274, 551, 837
BbsI GAAGAC 3 cut(s) 336, 1530, 1815
Bbv12I GWGCWC 4 cut(s) 837, 1360, 1763, 1853
BbvCI CCTCAGC 2 cut(s) 752, 1565
BbvI GCAGC 9 cut(s) 516, 698, 953, 1236, 1345, 1629, 1762, 2005, 2061
BccI CCATC 1 cut(s) 998
BceAI ACGGC 2 cut(s) 276, 1618
BciT130I CCWGG 4 cut(s) 374, 409, 875, 1673
BclI TGATCA 1 cut(s) 1465
BcnI CCSGG 3 cut(s) 546, 1214, 2050
BcoDI GTCTC 4 cut(s) 190, 384, 911, 1281
BfaI CTAG 4 cut(s) 1025, 1520, 1622, 2154
BfmI CTRYAG 2 cut(s) 939, 1305
BfoI RGCGCY 2 cut(s) 462, 733
BfuAI ACCTGC 1 cut(s) 1688
Bme1390I CCNGG 7 cut(s) 374, 409, 546, 875, 1214, 1673, 2050
Bme18I GGWCC 4 cut(s) 780, 871, 1065, 1498
BmeT110I CYCGRG 1 cut(s) 836
BmgT120I GGNCC 7 cut(s) 299, 547, 548, 780, 871, 1065, 1498
BmiI GGNNCC 5 cut(s) 63, 273, 549, 550, 2039
BmrFI CCNGG 7 cut(s) 374, 409, 546, 875, 1214, 1673, 2050
BmsI GCATC 7 cut(s) 706, 765, 1126, 1458, 1754, 1897, 2113
BoxI GACNNNNGTC 1 cut(s) 1425
BpiI GAAGAC 3 cut(s) 336, 1530, 1815
BplI GAGNNNNNCTC 4 cut(s) 566, 598, 1342, 1374
BpmI CTGGAG 2 cut(s) 222, 1032
Bpu10I CCTNAGC 2 cut(s) 752, 1565
Bpu14I TTCGAA 1 cut(s) 1292
BpuMI CCSGG 3 cut(s) 546, 1214, 2050
BsaAI YACGTR 1 cut(s) 604
BsaJI CCNNGG 2 cut(s) 408, 1250
BsaWI WCCGGW 1 cut(s) 1763
BsaXI ACNNNNNCTCC 4 cut(s) 220, 250, 616, 646
Bsc4I CCNNNNNNNGG 8 cut(s) 58, 132, 373, 545, 641, 1074, 1158, 1374
Bse118I RCCGGY 1 cut(s) 777
Bse1I ACTGG 2 cut(s) 137, 1067
Bse21I CCTNAGG 1 cut(s) 1752
Bse3DI GCAATG 1 cut(s) 1219
BseBI CCWGG 4 cut(s) 374, 409, 875, 1673
BseDI CCNNGG 2 cut(s) 408, 1250
BseGI GGATG 6 cut(s) 747, 756, 1020, 1117, 1449, 1699
BseLI CCNNNNNNNGG 8 cut(s) 58, 132, 373, 545, 641, 1074, 1158, 1374
BseMI GCAATG 1 cut(s) 1219
BseMII CTCAG 8 cut(s) 27, 151, 205, 766, 922, 1579, 1743, 2071
BseNI ACTGG 2 cut(s) 137, 1067
BseRI GAGGAG 2 cut(s) 525, 1366
BseSI GKGCMC 1 cut(s) 551
BseX3I CGGCCG 1 cut(s) 1631
BseXI GCAGC 9 cut(s) 516, 698, 953, 1236, 1345, 1629, 1762, 2005, 2061
Bsh1236I CGCG 1 cut(s) 560
Bsh1285I CGRYCG 1 cut(s) 1634
BshFI GGCC 5 cut(s) 86, 300, 549, 1547, 1633
BshNI GGYRCC 1 cut(s) 61
BsiEI CGRYCG 1 cut(s) 1634
BsiHKAI GWGCWC 4 cut(s) 837, 1360, 1763, 1853
BsiHKCI CYCGRG 1 cut(s) 836
BsiSI CCGG 5 cut(s) 545, 778, 1214, 1764, 2050
BslFI GGGAC 1 cut(s) 1286
BslI CCNNNNNNNGG 8 cut(s) 58, 132, 373, 545, 641, 1074, 1158, 1374
BsmAI GTCTC 4 cut(s) 190, 384, 911, 1281
BsmBI CGTCTC 2 cut(s) 190, 911
BsmFI GGGAC 1 cut(s) 1286
BsmI GAATGC 2 cut(s) 263, 1473
BsnI GGCC 5 cut(s) 86, 300, 549, 1547, 1633
BsoBI CYCGRG 1 cut(s) 836
Bsp119I TTCGAA 1 cut(s) 1292
Bsp120I GGGCCC 1 cut(s) 547
Bsp1286I GDGCHC 6 cut(s) 274, 551, 837, 1360, 1763, 1853
Bsp19I CCATGG 1 cut(s) 1250
BspACI CCGC 4 cut(s) 4, 869, 1630, 1691
BspANI GGCC 5 cut(s) 86, 300, 549, 1547, 1633
BspCNI CTCAG 8 cut(s) 26, 150, 204, 765, 921, 1578, 1744, 2070
BspFNI CGCG 1 cut(s) 560
BspLI GGNNCC 5 cut(s) 63, 273, 549, 550, 2039
BspMAI CTGCAG 1 cut(s) 943
BspMI ACCTGC 1 cut(s) 1688
BspPI GGATC 4 cut(s) 536, 792, 1240, 1661
BspT104I TTCGAA 1 cut(s) 1292
BspT107I GGYRCC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 1219
BsrFI RCCGGY 1 cut(s) 777
BsrI ACTGG 2 cut(s) 137, 1067
BssAI RCCGGY 1 cut(s) 777
BssECI CCNNGG 2 cut(s) 408, 1250
BssT1I CCWWGG 1 cut(s) 1250
Bst2UI CCWGG 4 cut(s) 374, 409, 875, 1673
Bst4CI ACNGT 3 cut(s) 184, 1179, 2124
BstBAI YACGTR 1 cut(s) 604
BstBI TTCGAA 1 cut(s) 1292
BstDEI CTNAG 8 cut(s) 13, 137, 191, 752, 908, 1565, 1752, 2057
BstDSI CCRYGG 1 cut(s) 1250
BstENI CCTNNNNNAGG 2 cut(s) 371, 639
BstF5I GGATG 6 cut(s) 747, 756, 1020, 1117, 1449, 1699
BstFNI CGCG 1 cut(s) 560
BstH2I RGCGCY 2 cut(s) 462, 733
BstHHI GCGC 4 cut(s) 461, 732, 1685, 2104
BstMAI GTCTC 4 cut(s) 190, 384, 911, 1281
BstMCI CGRYCG 1 cut(s) 1634
BstMWI GCNNNNNNNGC 6 cut(s) 269, 1364, 1639, 1742, 1832, 2059
BstNI CCWGG 4 cut(s) 374, 409, 875, 1673
BstNSI RCATGY 2 cut(s) 1003, 1914
BstPAI GACNNNNGTC 1 cut(s) 1425
BstSCI CCNGG 7 cut(s) 372, 407, 544, 873, 1212, 1671, 2048
BstSFI CTRYAG 2 cut(s) 939, 1305
BstSLI GKGCMC 1 cut(s) 551
BstUI CGCG 1 cut(s) 560
BstV1I GCAGC 9 cut(s) 516, 698, 953, 1236, 1345, 1629, 1762, 2005, 2061
BstV2I GAAGAC 3 cut(s) 336, 1530, 1815
BstX2I RGATCY 2 cut(s) 797, 1245
BstYI RGATCY 2 cut(s) 797, 1245
BstZI CGGCCG 1 cut(s) 1631
Bsu36I CCTNAGG 1 cut(s) 1752
BsuRI GGCC 5 cut(s) 86, 300, 549, 1547, 1633
BtgI CCRYGG 1 cut(s) 1250
BtgZI GCGATG 1 cut(s) 1731
BtsCI GGATG 6 cut(s) 747, 756, 1020, 1117, 1449, 1699
BtsIMutI CAGTG 1 cut(s) 1902
BveI ACCTGC 1 cut(s) 1688
CaiI CAGNNNCTG 3 cut(s) 132, 801, 1778
CfoI GCGC 4 cut(s) 461, 732, 1685, 2104
Cfr10I RCCGGY 1 cut(s) 777
Cfr13I GGNCC 7 cut(s) 299, 547, 548, 780, 871, 1065, 1498
Csp6I GTAC 3 cut(s) 62, 601, 1389
CviQI GTAC 3 cut(s) 62, 601, 1389
DdeI CTNAG 8 cut(s) 13, 137, 191, 752, 908, 1565, 1752, 2057
DraI TTTAAA 1 cut(s) 1705
EaeI YGGCCR 2 cut(s) 1545, 1631
EagI CGGCCG 1 cut(s) 1631
Ecl136II GAGCTC 1 cut(s) 835
EclXI CGGCCG 1 cut(s) 1631
Eco130I CCWWGG 1 cut(s) 1250
Eco24I GRGCYC 3 cut(s) 274, 551, 837
Eco47I GGWCC 4 cut(s) 780, 871, 1065, 1498
Eco52I CGGCCG 1 cut(s) 1631
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 2 cut(s) 648, 1890
Eco81I CCTNAGG 1 cut(s) 1752
Eco88I CYCGRG 1 cut(s) 836
EcoICRI GAGCTC 1 cut(s) 835
EcoNI CCTNNNNNAGG 2 cut(s) 371, 639
EcoO109I RGGNCCY 1 cut(s) 548
EcoRI GAATTC 2 cut(s) 1288, 1697
EcoRII CCWGG 4 cut(s) 372, 407, 873, 1671
EcoT14I CCWWGG 1 cut(s) 1250
EcoT22I ATGCAT 1 cut(s) 1912
EcoT38I GRGCYC 3 cut(s) 274, 551, 837
ErhI CCWWGG 1 cut(s) 1250
Esp3I CGTCTC 2 cut(s) 190, 911
FaqI GGGAC 1 cut(s) 1286
FbaI TGATCA 1 cut(s) 1465
FokI GGATG 6 cut(s) 734, 743, 1007, 1104, 1436, 1706
FriOI GRGCYC 3 cut(s) 274, 551, 837
FspBI CTAG 4 cut(s) 1025, 1520, 1622, 2154
GlaI GCGC 4 cut(s) 460, 731, 1684, 2103
GsuI CTGGAG 2 cut(s) 222, 1032
HaeII RGCGCY 2 cut(s) 462, 733
HaeIII GGCC 5 cut(s) 86, 300, 549, 1547, 1633
HapII CCGG 5 cut(s) 545, 778, 1214, 1764, 2050
HhaI GCGC 4 cut(s) 461, 732, 1685, 2104
Hin6I GCGC 4 cut(s) 459, 730, 1683, 2102
HinP1I GCGC 4 cut(s) 459, 730, 1683, 2102
HindIII AAGCTT 1 cut(s) 810
HinfI GANTC 5 cut(s) 564, 1370, 1668, 1781, 1963
HpaII CCGG 5 cut(s) 545, 778, 1214, 1764, 2050
HphI GGTGA 4 cut(s) 656, 1768, 1858, 2081
Hpy166II GTNNAC 1 cut(s) 880
Hpy188III TCNNGA 8 cut(s) 239, 539, 769, 790, 1270, 1412, 1430, 1520
Hpy8I GTNNAC 1 cut(s) 880
HpyAV CCTTC 5 cut(s) 590, 793, 1288, 1726, 1816
HpyCH4III ACNGT 3 cut(s) 184, 1179, 2124
HpyCH4IV ACGT 1 cut(s) 603
HpyF10VI GCNNNNNNNGC 6 cut(s) 269, 1364, 1639, 1742, 1832, 2059
HpyF3I CTNAG 8 cut(s) 13, 137, 191, 752, 908, 1565, 1752, 2057
HpySE526I ACGT 1 cut(s) 603
HspAI GCGC 4 cut(s) 459, 730, 1683, 2102
KpnI GGTACC 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 1465
LmnI GCTCC 6 cut(s) 277, 637, 670, 1013, 1363, 1446
Lsp1109I GCAGC 9 cut(s) 516, 698, 953, 1236, 1345, 1629, 1762, 2005, 2061
LweI GCATC 7 cut(s) 706, 765, 1126, 1458, 1754, 1897, 2113
MaeI CTAG 4 cut(s) 1025, 1520, 1622, 2154
MaeII ACGT 1 cut(s) 603
MaeIII GTNAC 3 cut(s) 256, 985, 1505
MflI RGATCY 2 cut(s) 797, 1245
MhlI GDGCHC 6 cut(s) 274, 551, 837, 1360, 1763, 1853
MlsI TGGCCA 1 cut(s) 1547
MluNI TGGCCA 1 cut(s) 1547
MlyI GAGTC 1 cut(s) 1379
MmeI TCCRAC 2 cut(s) 1424, 1559
Mox20I TGGCCA 1 cut(s) 1547
Mph1103I ATGCAT 1 cut(s) 1912
MroXI GAANNNNTTC 3 cut(s) 118, 968, 2124
MscI TGGCCA 1 cut(s) 1547
MseI TTAA 7 cut(s) 68, 426, 609, 716, 1187, 1704, 1860
MslI CAYNNNNRTG 1 cut(s) 998
Msp20I TGGCCA 1 cut(s) 1547
MspA1I CMGCKG 2 cut(s) 1775, 1868
MspI CCGG 5 cut(s) 545, 778, 1214, 1764, 2050
MspR9I CCNGG 7 cut(s) 374, 409, 546, 875, 1214, 1673, 2050
Mva1269I GAATGC 2 cut(s) 263, 1473
MvaI CCWGG 4 cut(s) 374, 409, 875, 1673
MvnI CGCG 1 cut(s) 560
MwoI GCNNNNNNNGC 6 cut(s) 269, 1364, 1639, 1742, 1832, 2059
NciI CCSGG 3 cut(s) 546, 1214, 2050
NcoI CCATGG 1 cut(s) 1250
NlaIV GGNNCC 5 cut(s) 63, 273, 549, 550, 2039
NmeAIII GCCGAG 1 cut(s) 1393
NmuCI GTSAC 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 1912
NspI RCATGY 2 cut(s) 1003, 1914
NspV TTCGAA 1 cut(s) 1292
PaeI GCATGC 1 cut(s) 1914
PaqCI CACCTGC 1 cut(s) 1688
PciI ACATGT 1 cut(s) 999
PctI GAATGC 2 cut(s) 263, 1473
PdmI GAANNNNTTC 3 cut(s) 118, 968, 2124
PfeI GAWTC 4 cut(s) 564, 1668, 1781, 1963
PflMI CCANNNNNTGG 3 cut(s) 132, 1074, 1158
PfoI TCCNGGA 1 cut(s) 1671
PleI GAGTC 1 cut(s) 1378
PpsI GAGTC 1 cut(s) 1378
Ppu21I YACGTR 1 cut(s) 604
PscI ACATGT 1 cut(s) 999
PshAI GACNNNNGTC 1 cut(s) 1425
PsiI TTATAA 1 cut(s) 1340
Psp124BI GAGCTC 1 cut(s) 837
Psp6I CCWGG 4 cut(s) 372, 407, 873, 1671
PspGI CCWGG 4 cut(s) 372, 407, 873, 1671
PspN4I GGNNCC 5 cut(s) 63, 273, 549, 550, 2039
PspOMI GGGCCC 1 cut(s) 547
PspPI GGNCC 7 cut(s) 299, 547, 548, 780, 871, 1065, 1498
PsrI GAACNNNNNNTAC 2 cut(s) 839, 871
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 3 cut(s) 132, 801, 1778
PsuI RGATCY 2 cut(s) 797, 1245
PvuII CAGCTG 2 cut(s) 1775, 1868
RsaI GTAC 3 cut(s) 63, 602, 1390
RsaNI GTAC 3 cut(s) 62, 601, 1389
RseI CAYNNNNRTG 1 cut(s) 998
SacI GAGCTC 1 cut(s) 837
SaqAI TTAA 7 cut(s) 68, 426, 609, 716, 1187, 1704, 1860
Sau96I GGNCC 7 cut(s) 299, 547, 548, 780, 871, 1065, 1498
SchI GAGTC 1 cut(s) 1379
ScrFI CCNGG 7 cut(s) 374, 409, 546, 875, 1214, 1673, 2050
SduI GDGCHC 6 cut(s) 274, 551, 837, 1360, 1763, 1853
SfaNI GCATC 7 cut(s) 706, 765, 1126, 1458, 1754, 1897, 2113
SfcI CTRYAG 2 cut(s) 939, 1305
SfuI TTCGAA 1 cut(s) 1292
SinI GGWCC 4 cut(s) 780, 871, 1065, 1498
SmiMI CAYNNNNRTG 1 cut(s) 998
SphI GCATGC 1 cut(s) 1914
SsiI CCGC 4 cut(s) 4, 869, 1630, 1691
SspI AATATT 1 cut(s) 1109
SspMI CTAG 4 cut(s) 1025, 1520, 1622, 2154
SstI GAGCTC 1 cut(s) 837
StyD4I CCNGG 7 cut(s) 372, 407, 544, 873, 1212, 1671, 2048
StyI CCWWGG 1 cut(s) 1250
TaaI ACNGT 3 cut(s) 184, 1179, 2124
TaiI ACGT 1 cut(s) 606
TaqI TCGA 4 cut(s) 957, 1292, 1349, 1573
TaqII GACCGA 1 cut(s) 1827
TatI WGTACW 1 cut(s) 1388
TauI GCSGC 1 cut(s) 1633
TfiI GAWTC 4 cut(s) 564, 1668, 1781, 1963
Tru1I TTAA 7 cut(s) 68, 426, 609, 716, 1187, 1704, 1860
Tru9I TTAA 7 cut(s) 68, 426, 609, 716, 1187, 1704, 1860
TscAI CASTG 1 cut(s) 1902
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 9 cut(s) 504, 686, 941, 1224, 1333, 1642, 1775, 2018, 2074
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 4 cut(s) 278, 1119, 1627, 1710
TspGWI ACGGA 3 cut(s) 41, 584, 1511
TspRI CASTG 1 cut(s) 1902
Van91I CCANNNNNTGG 3 cut(s) 132, 1074, 1158
VpaK11BI GGWCC 4 cut(s) 780, 871, 1065, 1498
XagI CCTNNNNNAGG 2 cut(s) 371, 639
XapI RAATTY 8 cut(s) 1265, 1288, 1382, 1460, 1534, 1558, 1697, 2130
XbaI TCTAGA 1 cut(s) 1519
XceI RCATGY 2 cut(s) 1003, 1914
XmnI GAANNNNTTC 3 cut(s) 118, 968, 2124
XspI CTAG 4 cut(s) 1025, 1520, 1622, 2154
Zsp2I ATGCAT 1 cut(s) 1912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.