FvH4_4g30270

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000618
Physical Location & Seq
Forward (+)
29987531 .. 29988266
736 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30270.t1

Sequence Viewer

Length: 486 bp
ATGCTGTATCTCCTAGTCATGTACCCCTCCATGCCACCGTGCTACCCAATGGCATCGGACAAATCAGGTTCCAAGACACATGCATGTGCCGAGGCCATTGAATTCTTTGAAGAAAGAAAATGCAAAGGAGACAAAAAGGCTAGCAAAGCATTACTTGAGGTGAGCACTGAAATACCTCTGGCTGAAATTAAAGGCGATAGAAGTAAGTCGGTGCTATTTGATGCCTGCAGGCTTGCAAAGACATTGCAATTGCTGGAAACAGAAGAAAAGTGGGACAACAAAAAGAAGTGGAAGGTGATAAGTCATGTGTGGGTGGAAATGCTATCTTATGCTGCAAGTCATTGTCCCTGGAATCAGCATGCTCGGCAACTTAGGCGGTGTGGAGAGCTGCTTACTCATGTGTGGCTTCTCATGGCACATCTTGGTATAACTGAACAGTTTCAAATTTCAAAGGGCCATGCTAGAGCTAAATGGATTGTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

162

Amino Acids

18.53

Weight (kDa)

9.01

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF594 PF04578 90 - 143 6.8e-25 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 376
AcsI RAATTY 2 cut(s) 101, 444
AfaI GTAC 1 cut(s) 23
AgsI TTSAA 4 cut(s) 101, 110, 443, 450
AjnI CCWGG 1 cut(s) 347
AluBI AGCT 2 cut(s) 388, 467
AluI AGCT 2 cut(s) 388, 467
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 1 cut(s) 123
AoxI GGCC 2 cut(s) 93, 454
ApeKI GCWGC 2 cut(s) 332, 388
ApoI RAATTY 2 cut(s) 101, 444
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 1 cut(s) 454
AsuHPI GGTGA 2 cut(s) 172, 307
AsuNHI GCTAGC 1 cut(s) 140
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 2 cut(s) 319, 375
BciT130I CCWGG 1 cut(s) 349
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 3 cut(s) 14, 141, 462
BfmI CTRYAG 1 cut(s) 226
BisI GCNGC 2 cut(s) 333, 389
BlsI GCNGC 2 cut(s) 334, 390
Bme1390I CCNGG 1 cut(s) 349
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 1 cut(s) 349
BmsI GCATC 2 cut(s) 62, 211
BmtI GCTAGC 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 90, 347
Bse3DI GCAATG 1 cut(s) 242
BseBI CCWGG 1 cut(s) 349
BseDI CCNNGG 2 cut(s) 90, 347
BseMI GCAATG 1 cut(s) 242
BseXI GCAGC 2 cut(s) 319, 375
BshFI GGCC 2 cut(s) 95, 456
BsiHKAI GWGCWC 1 cut(s) 167
BslFI GGGAC 2 cut(s) 287, 330
BsmAI GTCTC 1 cut(s) 123
BsmFI GGGAC 2 cut(s) 287, 330
BsnI GGCC 2 cut(s) 95, 456
Bsp1286I GDGCHC 1 cut(s) 167
BspACI CCGC 1 cut(s) 376
BspANI GGCC 2 cut(s) 95, 456
BspLI GGNNCC 1 cut(s) 70
BspMAI CTGCAG 1 cut(s) 230
BspOI GCTAGC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 242
BssECI CCNNGG 2 cut(s) 90, 347
Bst2UI CCWGG 1 cut(s) 349
Bst4CI ACNGT 2 cut(s) 39, 438
BstC8I GCNNGC 5 cut(s) 142, 226, 230, 234, 360
BstDEI CTNAG 1 cut(s) 371
BstMAI GTCTC 1 cut(s) 123
BstMWI GCNNNNNNNGC 3 cut(s) 146, 364, 373
BstNI CCWGG 1 cut(s) 349
BstNSI RCATGY 3 cut(s) 83, 87, 362
BstSCI CCNGG 1 cut(s) 347
BstSFI CTRYAG 1 cut(s) 226
BstV1I GCAGC 2 cut(s) 319, 375
BsuRI GGCC 2 cut(s) 95, 456
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 5 cut(s) 142, 226, 230, 234, 360
Cfr13I GGNCC 1 cut(s) 454
Csp6I GTAC 1 cut(s) 22
CviAII CATG 9 cut(s) 19, 31, 80, 84, 305, 359, 398, 412, 458
CviJI RGCY 8 cut(s) 95, 140, 182, 232, 388, 406, 456, 467
CviKI_1 RGCY 8 cut(s) 95, 140, 182, 232, 388, 406, 456, 467
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 1 cut(s) 371
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 347
EcoT22I ATGCAT 1 cut(s) 85
FaeI CATG 9 cut(s) 22, 34, 83, 87, 308, 362, 401, 415, 461
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FaqI GGGAC 2 cut(s) 287, 330
FatI CATG 9 cut(s) 18, 30, 79, 83, 304, 358, 397, 411, 457
Fnu4HI GCNGC 2 cut(s) 333, 389
Fsp4HI GCNGC 2 cut(s) 333, 389
FspBI CTAG 3 cut(s) 14, 141, 462
GluI GCNGC 2 cut(s) 333, 389
HaeIII GGCC 2 cut(s) 95, 456
Hin1II CATG 9 cut(s) 22, 34, 83, 87, 308, 362, 401, 415, 461
HinfI GANTC 1 cut(s) 352
HphI GGTGA 2 cut(s) 172, 307
Hpy188I TCNGA 1 cut(s) 58
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 2 cut(s) 39, 438
HpyCH4V TGCA 7 cut(s) 83, 123, 228, 236, 247, 335, 481
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 364, 373
HpyF3I CTNAG 1 cut(s) 371
Hsp92II CATG 9 cut(s) 22, 34, 83, 87, 308, 362, 401, 415, 461
LpnPI CCDG 7 cut(s) 51, 164, 214, 238, 239, 334, 361
Lsp1109I GCAGC 2 cut(s) 319, 375
LweI GCATC 2 cut(s) 62, 211
MaeI CTAG 3 cut(s) 14, 141, 462
MboII GAAGA 2 cut(s) 122, 275
MfeI CAATTG 1 cut(s) 248
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 4 cut(s) 101, 186, 248, 444
MnlI CCTC 4 cut(s) 37, 85, 151, 186
Mph1103I ATGCAT 1 cut(s) 85
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 2 cut(s) 82, 84
MspR9I CCNGG 1 cut(s) 349
MunI CAATTG 1 cut(s) 248
MvaI CCWGG 1 cut(s) 349
MwoI GCNNNNNNNGC 3 cut(s) 146, 364, 373
NheI GCTAGC 1 cut(s) 140
NlaIII CATG 9 cut(s) 22, 34, 83, 87, 308, 362, 401, 415, 461
NlaIV GGNNCC 1 cut(s) 70
NmeAIII GCCGAG 2 cut(s) 115, 343
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 3 cut(s) 83, 87, 362
PaeI GCATGC 1 cut(s) 362
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 1 cut(s) 352
PkrI GCNGC 2 cut(s) 334, 390
Psp6I CCWGG 1 cut(s) 347
PspGI CCWGG 1 cut(s) 347
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 1 cut(s) 454
PstI CTGCAG 1 cut(s) 230
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
RseI CAYNNNNRTG 2 cut(s) 82, 84
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 2 cut(s) 333, 389
Sau96I GGNCC 1 cut(s) 454
SbfI CCTGCAGG 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 349
SdaI CCTGCAGG 1 cut(s) 230
SduI GDGCHC 1 cut(s) 167
SetI ASST 6 cut(s) 70, 162, 178, 297, 390, 469
SfaNI GCATC 2 cut(s) 62, 211
SfcI CTRYAG 1 cut(s) 226
SmiMI CAYNNNNRTG 2 cut(s) 82, 84
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
SphI GCATGC 1 cut(s) 362
Sse8387I CCTGCAGG 1 cut(s) 230
Sse9I AATT 4 cut(s) 101, 186, 248, 444
SsiI CCGC 1 cut(s) 376
SspMI CTAG 3 cut(s) 14, 141, 462
StyD4I CCNGG 1 cut(s) 347
TaaI ACNGT 2 cut(s) 39, 438
TasI AATT 4 cut(s) 101, 186, 248, 444
TfiI GAWTC 1 cut(s) 352
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 172
TseI GCWGC 2 cut(s) 332, 388
TspRI CASTG 1 cut(s) 172
XapI RAATTY 2 cut(s) 101, 444
XceI RCATGY 3 cut(s) 83, 87, 362
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 3 cut(s) 14, 141, 462
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.