pycom09g10050

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000618
Physical Location & Seq
Reverse (-)
8131408 .. 8132619
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10050.1

Sequence Viewer

Length: 1212 bp
ATGTATGATACGTTTTATACTAAAGCCGTCCTGGTTTATTCTCGTATGGGTGGTATTCTCCGTTGTATCACTCTTACCGTAACAATTTCTGTACTCGTTGCCTTCCCTTTCACGACAAAGCCAGAATACAAGGTAGTGGATTTAGTGATCACATACATGTTGTTGGGAGGAGCTATAGTCCTAGAAATTTATGCTATAGTTATAATGCTTTCCTCAGACTGGACAATGCTATGGATGAGTAAGCACAAATTTATGGTGCTGGATCGCAGAGCGGTTTCATCAATTCCCTTGGTGAAAAACAAGAGGTGGTCGAATAGCGTGGCACAATACAACTTGTTCATGTTCTGTCTTAAAGTACGGCCGCAGAAGTTTATTGTTATCCAGAAAGTTTTATGCATAGATCAGTTGTTAGAATACTACCGATACAAAGACTCGAAGGATGTTTCGGGAGAGTTGAAAGGTCTGATCTTTGAGCAACTCAGGGAGAAATCAAGGATGGCTTCCGAATTTGGCGCGTGCAAAAAACTGTGCGCTCGAAGGGGAGACCAGGTGCTTGAAAAAGCAGATTGTCTCAATAAACTTGGCTGGAGCATCAACGGGGTTGAATTTGACCAAAGCATCCTTCTCTGGCATATTGCAACAGATCTATGTTATCATTCTGATATGAACAGAAATTTGGACTGTGGTAGTAACCCGAATTGTGAAGCCAGTAAGCTCCTATCAAACTATATGTTGTATCTTTTAGTCATGTGCCCCTTCATGCTGCCAAATGGGATTGGACAGATTAGATTCAGAGACACATGTGCAGAGACTGAAGAGTTTTTTAAGGAAAAAAATTTACTGAAATCAGATGCGGAGAGAGCTTGCACGAAGCTCTTCAATGTGAGCACAGACTTCCTTCCTTTGAAAGTGAAAGGAGATAGAAGCAAGTCGGTCCTATTTGATGCATGCAGGCTTGCTAAGGCTTTGCAGTCCCTGGAGTCTGAGGTGCATTGGGAAGACAATAAGGAAAAGTGGGAATTTATAAGTCATGTGTGGGTGGAAATGCTCTCTTATGCGGCCAATCAGTGTCGATGGAAGGATCACGGTCCGCAGCTCCGGCGAGGAGGAGAGCTGCTCACTCATGTCTGGCTTCTTATGGCACATCTTGGTTTAACTGAACAGTTCCAAATTTCAGAGGGGCAAGCCAGGCCAAAGTTGATTGCGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

404

Amino Acids

46.57

Weight (kDa)

8.79

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 1 - 113 5.7e-30 Domain of unknown function (DUF4220)
DUF594 PF04578 333 - 385 2.2e-23 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 203, 1025
AccBSI CCGCTC 1 cut(s) 272
AccII CGCG 1 cut(s) 515
AciI CCGC 5 cut(s) 272, 362, 854, 1058, 1091
AclWI GGATC 2 cut(s) 270, 1089
AcoI YGGCCR 2 cut(s) 359, 1059
AcsI RAATTY 8 cut(s) 186, 248, 506, 605, 673, 835, 1019, 1170
AcuI CTGAAG 1 cut(s) 834
AfaI GTAC 2 cut(s) 93, 357
AfiI CCNNNNNNNGG 1 cut(s) 219
AflIII ACRYGT 2 cut(s) 156, 800
AgsI TTSAA 5 cut(s) 457, 557, 605, 880, 907
AjnI CCWGG 4 cut(s) 30, 546, 975, 1187
AjuI GAANNNNNNNTTGG 4 cut(s) 606, 638, 659, 691
AluBI AGCT 6 cut(s) 173, 715, 863, 874, 1096, 1114
AluI AGCT 6 cut(s) 173, 715, 863, 874, 1096, 1114
Alw21I GWGCWC 1 cut(s) 890
Alw26I GTCTC 4 cut(s) 537, 575, 789, 803
AlwI GGATC 2 cut(s) 270, 1089
AlwNI CAGNNNCTG 2 cut(s) 812, 976
AoxI GGCC 3 cut(s) 359, 1059, 1190
ApeKI GCWGC 3 cut(s) 763, 1093, 1114
ApoI RAATTY 8 cut(s) 186, 248, 506, 605, 673, 835, 1019, 1170
Asp700I GAANNNNTTC 1 cut(s) 875
AspLEI GCGC 2 cut(s) 515, 533
AspS9I GGNCC 2 cut(s) 934, 1088
AsuHPI GGTGA 1 cut(s) 304
AvaII GGWCC 2 cut(s) 934, 1088
BaeGI GKGCMC 1 cut(s) 755
BbsI GAAGAC 1 cut(s) 1005
Bbv12I GWGCWC 1 cut(s) 890
BbvI GCAGC 3 cut(s) 750, 1101, 1105
BccI CCATC 2 cut(s) 490, 1068
BceAI ACGGC 2 cut(s) 11, 374
BciT130I CCWGG 4 cut(s) 32, 548, 977, 1189
BclI TGATCA 1 cut(s) 147
BcoDI GTCTC 4 cut(s) 537, 575, 789, 803
BfaI CTAG 1 cut(s) 182
BfmI CTRYAG 2 cut(s) 174, 195
BglII AGATCT 1 cut(s) 643
BisI GCNGC 5 cut(s) 362, 764, 1059, 1094, 1115
BlsI GCNGC 5 cut(s) 363, 765, 1060, 1095, 1116
Bme1390I CCNGG 4 cut(s) 32, 548, 977, 1189
Bme18I GGWCC 2 cut(s) 934, 1088
BmgT120I GGNCC 2 cut(s) 934, 1088
BmrFI CCNGG 4 cut(s) 32, 548, 977, 1189
BmsI GCATC 4 cut(s) 600, 627, 841, 934
BpiI GAAGAC 1 cut(s) 1005
BplI GAGNNNNNCTC 2 cut(s) 1101, 1133
BpmI CTGGAG 2 cut(s) 607, 998
Bpu10I CCTNAGC 1 cut(s) 960
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 2 cut(s) 288, 975
BsaXI ACNNNNNCTCC 4 cut(s) 162, 192, 534, 564
Bsc4I CCNNNNNNNGG 1 cut(s) 219
Bse1I ACTGG 2 cut(s) 224, 708
BseBI CCWGG 4 cut(s) 32, 548, 977, 1189
BseDI CCNNGG 2 cut(s) 288, 975
BseGI GGATG 4 cut(s) 240, 445, 501, 618
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMII CTCAG 3 cut(s) 228, 493, 975
BseNI ACTGG 2 cut(s) 224, 708
BseRI GAGGAG 3 cut(s) 183, 1119, 1122
BseSI GKGCMC 1 cut(s) 755
BseX3I CGGCCG 1 cut(s) 359
BseXI GCAGC 3 cut(s) 750, 1101, 1105
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 1 cut(s) 515
Bsh1285I CGRYCG 1 cut(s) 362
BshFI GGCC 3 cut(s) 361, 1061, 1192
BsiEI CGRYCG 1 cut(s) 362
BsiHKAI GWGCWC 1 cut(s) 890
BsiSI CCGG 1 cut(s) 1099
BslFI GGGAC 1 cut(s) 958
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 4 cut(s) 537, 575, 789, 803
BsmFI GGGAC 1 cut(s) 958
BsnI GGCC 3 cut(s) 361, 1061, 1192
Bso31I GGTCTC 1 cut(s) 537
Bsp1286I GDGCHC 2 cut(s) 755, 890
Bsp143I GATC 6 cut(s) 147, 262, 400, 465, 643, 1081
BspACI CCGC 5 cut(s) 272, 362, 854, 1058, 1091
BspANI GGCC 3 cut(s) 361, 1061, 1192
BspCNI CTCAG 3 cut(s) 227, 492, 976
BspFNI CGCG 1 cut(s) 515
BspPI GGATC 2 cut(s) 270, 1089
BspQI GCTCTTC 1 cut(s) 881
BspTNI GGTCTC 1 cut(s) 537
BsrBI CCGCTC 1 cut(s) 272
BsrI ACTGG 2 cut(s) 224, 708
BssECI CCNNGG 2 cut(s) 288, 975
BssMI GATC 6 cut(s) 147, 262, 400, 465, 643, 1081
BssT1I CCWWGG 1 cut(s) 288
Bst2UI CCWGG 4 cut(s) 32, 548, 977, 1189
Bst4CI ACNGT 5 cut(s) 79, 528, 683, 1088, 1164
Bst6I CTCTTC 2 cut(s) 810, 881
BstC8I GCNNGC 6 cut(s) 517, 865, 949, 953, 957, 1185
BstDEI CTNAG 4 cut(s) 214, 479, 960, 984
BstF5I GGATG 4 cut(s) 240, 445, 501, 618
BstFNI CGCG 1 cut(s) 515
BstHHI GCGC 2 cut(s) 515, 533
BstKTI GATC 6 cut(s) 150, 265, 403, 468, 646, 1084
BstMAI GTCTC 4 cut(s) 537, 575, 789, 803
BstMBI GATC 6 cut(s) 147, 262, 400, 465, 643, 1081
BstMCI CGRYCG 1 cut(s) 362
BstMWI GCNNNNNNNGC 3 cut(s) 860, 1099, 1189
BstNI CCWGG 4 cut(s) 32, 548, 977, 1189
BstNSI RCATGY 3 cut(s) 160, 804, 951
BstSCI CCNGG 4 cut(s) 30, 546, 975, 1187
BstSFI CTRYAG 2 cut(s) 174, 195
BstSLI GKGCMC 1 cut(s) 755
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 3 cut(s) 750, 1101, 1105
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 1 cut(s) 643
BstYI RGATCY 1 cut(s) 643
BstZI CGGCCG 1 cut(s) 359
BsuRI GGCC 3 cut(s) 361, 1061, 1192
BtsCI GGATG 4 cut(s) 240, 445, 501, 618
BtsIMutI CAGTG 1 cut(s) 1073
Cac8I GCNNGC 6 cut(s) 517, 865, 949, 953, 957, 1185
CaiI CAGNNNCTG 2 cut(s) 812, 976
CfoI GCGC 2 cut(s) 515, 533
Cfr13I GGNCC 2 cut(s) 934, 1088
CpoI CGGWCCG 1 cut(s) 1088
CsiI ACCWGGT 1 cut(s) 546
Csp6I GTAC 2 cut(s) 92, 356
CspI CGGWCCG 1 cut(s) 1088
CviAII CATG 8 cut(s) 157, 340, 748, 760, 801, 948, 1031, 1124
CviQI GTAC 2 cut(s) 92, 356
DdeI CTNAG 4 cut(s) 214, 479, 960, 984
DpnI GATC 6 cut(s) 149, 264, 402, 467, 645, 1083
DpnII GATC 6 cut(s) 147, 262, 400, 465, 643, 1081
EaeI YGGCCR 2 cut(s) 359, 1059
EagI CGGCCG 1 cut(s) 359
Eam1104I CTCTTC 2 cut(s) 810, 881
EarI CTCTTC 2 cut(s) 810, 881
EclXI CGGCCG 1 cut(s) 359
Eco130I CCWWGG 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 537
Eco47I GGWCC 2 cut(s) 934, 1088
Eco52I CGGCCG 1 cut(s) 359
Eco57I CTGAAG 1 cut(s) 834
EcoRII CCWGG 4 cut(s) 30, 546, 975, 1187
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 2 cut(s) 398, 949
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 8 cut(s) 160, 343, 751, 763, 804, 951, 1034, 1127
FalI AAGNNNNNCTT 2 cut(s) 484, 516
FaqI GGGAC 1 cut(s) 958
FatI CATG 8 cut(s) 156, 339, 747, 759, 800, 947, 1030, 1123
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 5 cut(s) 362, 764, 1059, 1094, 1115
FokI GGATG 4 cut(s) 247, 452, 508, 605
Fsp4HI GCNGC 5 cut(s) 362, 764, 1059, 1094, 1115
FspBI CTAG 1 cut(s) 182
GlaI GCGC 2 cut(s) 514, 532
GluI GCNGC 5 cut(s) 362, 764, 1059, 1094, 1115
GsuI CTGGAG 2 cut(s) 607, 998
HaeIII GGCC 3 cut(s) 361, 1061, 1192
HapII CCGG 1 cut(s) 1099
HhaI GCGC 2 cut(s) 515, 533
Hin1II CATG 8 cut(s) 160, 343, 751, 763, 804, 951, 1034, 1127
Hin6I GCGC 2 cut(s) 513, 531
HinP1I GCGC 2 cut(s) 513, 531
HinfI GANTC 3 cut(s) 431, 789, 980
HpaII CCGG 1 cut(s) 1099
HphI GGTGA 1 cut(s) 304
Hpy188I TCNGA 8 cut(s) 217, 465, 505, 661, 794, 850, 985, 1177
Hpy188III TCNNGA 3 cut(s) 112, 382, 447
HpyAV CCTTC 7 cut(s) 112, 430, 531, 632, 766, 908, 1072
HpyCH4III ACNGT 5 cut(s) 79, 528, 683, 1088, 1164
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 9 cut(s) 396, 519, 638, 806, 867, 947, 951, 970, 991
HpyF10VI GCNNNNNNNGC 3 cut(s) 860, 1099, 1189
HpyF3I CTNAG 4 cut(s) 214, 479, 960, 984
HpySE526I ACGT 1 cut(s) 11
Hsp92II CATG 8 cut(s) 160, 343, 751, 763, 804, 951, 1034, 1127
HspAI GCGC 2 cut(s) 513, 531
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 6 cut(s) 147, 262, 400, 465, 643, 1081
LguI GCTCTTC 1 cut(s) 881
LmnI GCTCC 4 cut(s) 170, 588, 720, 1101
Lsp1109I GCAGC 3 cut(s) 750, 1101, 1105
LweI GCATC 4 cut(s) 600, 627, 841, 934
MabI ACCWGGT 1 cut(s) 546
MaeI CTAG 1 cut(s) 182
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 2 cut(s) 79, 689
MalI GATC 6 cut(s) 149, 264, 402, 467, 645, 1083
MbiI CCGCTC 1 cut(s) 272
MboI GATC 6 cut(s) 147, 262, 400, 465, 643, 1081
MboII GAAGA 3 cut(s) 827, 868, 1010
MflI RGATCY 1 cut(s) 643
MhlI GDGCHC 2 cut(s) 755, 890
MlyI GAGTC 2 cut(s) 425, 989
MnlI CCTC 8 cut(s) 161, 223, 297, 979, 1097, 1100, 1171, 1200
Mph1103I ATGCAT 2 cut(s) 398, 949
MroXI GAANNNNTTC 1 cut(s) 875
MseI TTAA 3 cut(s) 351, 825, 1154
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 1 cut(s) 1099
MspR9I CCNGG 4 cut(s) 32, 548, 977, 1189
MvaI CCWGG 4 cut(s) 32, 548, 977, 1189
MvnI CGCG 1 cut(s) 515
MwoI GCNNNNNNNGC 3 cut(s) 860, 1099, 1189
NdeII GATC 6 cut(s) 147, 262, 400, 465, 643, 1081
NlaIII CATG 8 cut(s) 160, 343, 751, 763, 804, 951, 1034, 1127
NsiI ATGCAT 2 cut(s) 398, 949
NspI RCATGY 3 cut(s) 160, 804, 951
PaeI GCATGC 1 cut(s) 951
PciI ACATGT 2 cut(s) 156, 800
PciSI GCTCTTC 1 cut(s) 881
PdmI GAANNNNTTC 1 cut(s) 875
PfeI GAWTC 1 cut(s) 789
PkrI GCNGC 5 cut(s) 363, 765, 1060, 1095, 1116
PleI GAGTC 2 cut(s) 425, 988
PpsI GAGTC 2 cut(s) 425, 988
PscI ACATGT 2 cut(s) 156, 800
PsiI TTATAA 2 cut(s) 203, 1025
Psp6I CCWGG 4 cut(s) 30, 546, 975, 1187
PspGI CCWGG 4 cut(s) 30, 546, 975, 1187
PspPI GGNCC 2 cut(s) 934, 1088
PstNI CAGNNNCTG 2 cut(s) 812, 976
PsuI RGATCY 1 cut(s) 643
RsaI GTAC 2 cut(s) 93, 357
RsaNI GTAC 2 cut(s) 92, 356
RseI CAYNNNNRTG 1 cut(s) 155
Rsr2I CGGWCCG 1 cut(s) 1088
RsrII CGGWCCG 1 cut(s) 1088
SapI GCTCTTC 1 cut(s) 881
SaqAI TTAA 3 cut(s) 351, 825, 1154
SatI GCNGC 5 cut(s) 362, 764, 1059, 1094, 1115
Sau3AI GATC 6 cut(s) 147, 262, 400, 465, 643, 1081
Sau96I GGNCC 2 cut(s) 934, 1088
SchI GAGTC 2 cut(s) 425, 989
ScrFI CCNGG 4 cut(s) 32, 548, 977, 1189
SduI GDGCHC 2 cut(s) 755, 890
SexAI ACCWGGT 1 cut(s) 546
SfaNI GCATC 4 cut(s) 600, 627, 841, 934
SfcI CTRYAG 2 cut(s) 174, 195
SinI GGWCC 2 cut(s) 934, 1088
SmiMI CAYNNNNRTG 1 cut(s) 155
SphI GCATGC 1 cut(s) 951
SsiI CCGC 5 cut(s) 272, 362, 854, 1058, 1091
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 4 cut(s) 30, 546, 975, 1187
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 5 cut(s) 79, 528, 683, 1088, 1164
TaiI ACGT 1 cut(s) 14
TaqI TCGA 4 cut(s) 311, 434, 535, 1072
TaqII GACCGA 1 cut(s) 922
TatI WGTACW 1 cut(s) 91
TauI GCSGC 2 cut(s) 364, 1061
TfiI GAWTC 1 cut(s) 789
Tru1I TTAA 3 cut(s) 351, 825, 1154
Tru9I TTAA 3 cut(s) 351, 825, 1154
TscAI CASTG 1 cut(s) 1073
TseI GCWGC 3 cut(s) 763, 1093, 1114
TspDTI ATGAA 4 cut(s) 267, 328, 680, 748
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 1 cut(s) 1073
VpaK11BI GGWCC 2 cut(s) 934, 1088
XapI RAATTY 8 cut(s) 186, 248, 506, 605, 673, 835, 1019, 1170
XceI RCATGY 3 cut(s) 160, 804, 951
XmnI GAANNNNTTC 1 cut(s) 875
XspI CTAG 1 cut(s) 182
Zsp2I ATGCAT 2 cut(s) 398, 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.