Rmu_sc0002599.1_g000001

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002599.1
OG0000618
Physical Location & Seq
Forward (+)
3114 .. 5116
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002599.1_g000001.1.cds

Sequence Viewer

Length: 627 bp
atgcagagggttaggttgcccattgctgaagagggccatgaggaagaaagagggtttcgccggagattgagaaagaaaatgggctcgatgggcgcgacagtggtgctggttgaactgatcgaccggtgtgactgggttgcaacactctcactcagcctacttcccagcaattcgatagaccatgatgcgaattcaaaagacctagactacatcatcatagcattttggggtccatttcttcttcttcacctcggtgggccagacaccattaccgcgtcatctttggaagacaatgagttgtggtcgaggtacttccttgtgctactagtccaggttgcagtcgctttctacatctaccttagagcctggggtgagagtctagctatgctccctcctcctgatccaggccccaactacgccagatacatgaatgagttccgctctaaggaatctgaaggcttcaaggttgagttagggagagtgattgaaactcctaaagtcggttattattcccatacagctccaagaaacatttttccgaatgcagccaatctgcaagatgcccatcttttcttcaatatcttcaaagcgctgtttgctgatctcatactcgatcaacatccatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

208

Amino Acids

23.72

Weight (kDa)

5.68

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 441
AccII CGCG 2 cut(s) 95, 275
AciI CCGC 2 cut(s) 273, 439
AclWI GGATC 1 cut(s) 395
AcsI RAATTY 1 cut(s) 190
AcuI CTGAAG 2 cut(s) 48, 474
AfaI GTAC 1 cut(s) 311
AfeI AGCGCT 1 cut(s) 591
AfiI CCNNNNNNNGG 3 cut(s) 404, 445, 500
AgeI ACCGGT 1 cut(s) 123
AgsI TTSAA 6 cut(s) 113, 195, 463, 488, 577, 586
AhlI ACTAGT 1 cut(s) 325
AjnI CCWGG 3 cut(s) 330, 365, 403
AleI CACNNNNGTG 1 cut(s) 252
AluBI AGCT 2 cut(s) 383, 521
AluI AGCT 2 cut(s) 383, 521
AlwI GGATC 1 cut(s) 395
Aor51HI AGCGCT 1 cut(s) 591
AoxI GGCC 3 cut(s) 34, 257, 406
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 1 cut(s) 190
AsiGI ACCGGT 1 cut(s) 123
Asp700I GAANNNNTTC 1 cut(s) 434
AspLEI GCGC 2 cut(s) 95, 592
AspS9I GGNCC 4 cut(s) 34, 230, 257, 407
AsuHPI GGTGA 2 cut(s) 239, 383
AvaII GGWCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 557
BccI CCATC 2 cut(s) 82, 573
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 3 cut(s) 332, 367, 405
BcuI ACTAGT 1 cut(s) 325
BfaI CTAG 3 cut(s) 203, 326, 380
BfoI RGCGCY 1 cut(s) 593
BisI GCNGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 547
Bme1390I CCNGG 3 cut(s) 332, 367, 405
Bme18I GGWCC 1 cut(s) 230
BmgT120I GGNCC 4 cut(s) 34, 230, 257, 407
BmiI GGNNCC 2 cut(s) 231, 409
BmrFI CCNGG 3 cut(s) 332, 367, 405
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 175, 550
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 294
BplI GAGNNNNNCTC 2 cut(s) 425, 457
BsaBI GATNNNNATC 2 cut(s) 564, 618
BsaJI CCNNGG 2 cut(s) 250, 366
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 3 cut(s) 404, 445, 500
Bse118I RCCGGY 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 137
Bse3DI GCAATG 1 cut(s) 21
Bse8I GATNNNNATC 2 cut(s) 564, 618
BseBI CCWGG 3 cut(s) 332, 367, 405
BseDI CCNNGG 2 cut(s) 250, 366
BseGI GGATG 1 cut(s) 619
BseJI GATNNNNATC 2 cut(s) 564, 618
BseLI CCNNNNNNNGG 3 cut(s) 404, 445, 500
BseMI GCAATG 1 cut(s) 21
BseMII CTCAG 1 cut(s) 166
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 1 cut(s) 384
BseXI GCAGC 1 cut(s) 557
BseYI CCCAGC 1 cut(s) 164
Bsh1236I CGCG 2 cut(s) 95, 275
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 3 cut(s) 36, 259, 408
BshTI ACCGGT 1 cut(s) 123
BsiEI CGRYCG 1 cut(s) 124
BsiSI CCGG 2 cut(s) 61, 124
BslI CCNNNNNNNGG 3 cut(s) 404, 445, 500
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 3 cut(s) 36, 259, 408
Bsp1286I GDGCHC 1 cut(s) 86
Bsp143I GATC 4 cut(s) 117, 400, 601, 613
BspACI CCGC 2 cut(s) 273, 439
BspANI GGCC 3 cut(s) 36, 259, 408
BspCNI CTCAG 1 cut(s) 165
BspFNI CGCG 2 cut(s) 95, 275
BspLI GGNNCC 2 cut(s) 231, 409
BspPI GGATC 1 cut(s) 395
BsrBI CCGCTC 1 cut(s) 441
BsrDI GCAATG 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 123
BsrI ACTGG 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 123
BssECI CCNNGG 2 cut(s) 250, 366
BssMI GATC 4 cut(s) 117, 400, 601, 613
Bst2UI CCWGG 3 cut(s) 332, 367, 405
Bst4CI ACNGT 1 cut(s) 100
Bst6I CTCTTC 1 cut(s) 24
BstDEI CTNAG 3 cut(s) 152, 359, 444
BstENI CCTNNNNNAGG 1 cut(s) 402
BstF5I GGATG 1 cut(s) 619
BstFNI CGCG 2 cut(s) 95, 275
BstH2I RGCGCY 1 cut(s) 593
BstHHI GCGC 2 cut(s) 95, 592
BstKTI GATC 4 cut(s) 120, 403, 604, 616
BstMBI GATC 4 cut(s) 117, 400, 601, 613
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 2 cut(s) 90, 596
BstNI CCWGG 3 cut(s) 332, 367, 405
BstSCI CCNGG 3 cut(s) 330, 365, 403
BstUI CGCG 2 cut(s) 95, 275
BstV1I GCAGC 1 cut(s) 557
BstV2I GAAGAC 1 cut(s) 294
BsuRI GGCC 3 cut(s) 36, 259, 408
BtsCI GGATG 1 cut(s) 619
BtsIMutI CAGTG 1 cut(s) 105
CfoI GCGC 2 cut(s) 95, 592
Cfr10I RCCGGY 1 cut(s) 123
Cfr13I GGNCC 4 cut(s) 34, 230, 257, 407
CseI GACGC 1 cut(s) 264
Csp6I GTAC 1 cut(s) 310
CspAI ACCGGT 1 cut(s) 123
CviAII CATG 4 cut(s) 38, 182, 427, 624
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 3 cut(s) 152, 359, 444
DpnI GATC 4 cut(s) 119, 402, 603, 615
DpnII GATC 4 cut(s) 117, 400, 601, 613
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Eco24I GRGCYC 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 230
Eco47III AGCGCT 1 cut(s) 591
Eco57I CTGAAG 2 cut(s) 48, 474
EcoNI CCTNNNNNAGG 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 190
EcoRII CCWGG 3 cut(s) 330, 365, 403
EcoT38I GRGCYC 1 cut(s) 86
FaeI CATG 4 cut(s) 41, 185, 430, 627
FaiI YATR 8 cut(s) 39, 183, 218, 386, 428, 516, 608, 625
FatI CATG 4 cut(s) 37, 181, 426, 623
Fnu4HI GCNGC 1 cut(s) 546
FokI GGATG 1 cut(s) 606
FriOI GRGCYC 1 cut(s) 86
Fsp4HI GCNGC 1 cut(s) 546
FspBI CTAG 3 cut(s) 203, 326, 380
GlaI GCGC 2 cut(s) 94, 591
GluI GCNGC 1 cut(s) 546
GsaI CCCAGC 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 593
HaeIII GGCC 3 cut(s) 36, 259, 408
HapII CCGG 2 cut(s) 61, 124
HgaI GACGC 1 cut(s) 264
HhaI GCGC 2 cut(s) 95, 592
Hin1II CATG 4 cut(s) 41, 185, 430, 627
Hin6I GCGC 2 cut(s) 93, 590
HinP1I GCGC 2 cut(s) 93, 590
HinfI GANTC 2 cut(s) 376, 449
HpaII CCGG 2 cut(s) 61, 124
HphI GGTGA 2 cut(s) 239, 383
Hpy188I TCNGA 2 cut(s) 454, 540
Hpy188III TCNNGA 1 cut(s) 398
HpyAV CCTTC 1 cut(s) 449
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4V TGCA 5 cut(s) 4, 140, 338, 545, 556
HpyF10VI GCNNNNNNNGC 2 cut(s) 90, 596
HpyF3I CTNAG 3 cut(s) 152, 359, 444
Hsp92II CATG 4 cut(s) 41, 185, 430, 627
HspAI GCGC 2 cut(s) 93, 590
Kzo9I GATC 4 cut(s) 117, 400, 601, 613
LmnI GCTCC 2 cut(s) 393, 526
Lsp1109I GCAGC 1 cut(s) 557
LweI GCATC 2 cut(s) 175, 550
MaeI CTAG 3 cut(s) 203, 326, 380
MaeIII GTNAC 1 cut(s) 128
MalI GATC 4 cut(s) 119, 402, 603, 615
MbiI CCGCTC 1 cut(s) 441
MboI GATC 4 cut(s) 117, 400, 601, 613
MboII GAAGA 8 cut(s) 41, 56, 230, 233, 236, 299, 565, 574
MhlI GDGCHC 1 cut(s) 86
MluCI AATT 2 cut(s) 169, 190
MlyI GAGTC 1 cut(s) 385
MnlI CCTC 7 cut(s) 25, 34, 44, 260, 300, 402, 405
MroXI GAANNNNTTC 1 cut(s) 434
MslI CAYNNNNRTG 1 cut(s) 252
MspI CCGG 2 cut(s) 61, 124
MspR9I CCNGG 3 cut(s) 332, 367, 405
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 3 cut(s) 332, 367, 405
MvnI CGCG 2 cut(s) 95, 275
MwoI GCNNNNNNNGC 2 cut(s) 90, 596
NdeII GATC 4 cut(s) 117, 400, 601, 613
NlaIII CATG 4 cut(s) 41, 185, 430, 627
NlaIV GGNNCC 2 cut(s) 231, 409
NmuCI GTSAC 1 cut(s) 128
OliI CACNNNNGTG 1 cut(s) 252
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PctI GAATGC 1 cut(s) 547
PdmI GAANNNNTTC 1 cut(s) 434
PfeI GAWTC 1 cut(s) 449
PinAI ACCGGT 1 cut(s) 123
PkrI GCNGC 1 cut(s) 547
PleI GAGTC 1 cut(s) 384
PpsI GAGTC 1 cut(s) 384
Psp6I CCWGG 3 cut(s) 330, 365, 403
PspFI CCCAGC 1 cut(s) 164
PspGI CCWGG 3 cut(s) 330, 365, 403
PspN4I GGNNCC 2 cut(s) 231, 409
PspPI GGNCC 4 cut(s) 34, 230, 257, 407
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 252
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 4 cut(s) 117, 400, 601, 613
Sau96I GGNCC 4 cut(s) 34, 230, 257, 407
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 3 cut(s) 332, 367, 405
SduI GDGCHC 1 cut(s) 86
SetI ASST 9 cut(s) 17, 204, 252, 311, 336, 360, 385, 468, 523
SfaNI GCATC 2 cut(s) 175, 550
SinI GGWCC 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 252
SpeI ACTAGT 1 cut(s) 325
Sse9I AATT 2 cut(s) 169, 190
SsiI CCGC 2 cut(s) 273, 439
SspMI CTAG 3 cut(s) 203, 326, 380
StyD4I CCNGG 3 cut(s) 330, 365, 403
TaaI ACNGT 1 cut(s) 100
TaqI TCGA 5 cut(s) 86, 120, 173, 305, 612
TasI AATT 2 cut(s) 169, 190
TfiI GAWTC 1 cut(s) 449
TscAI CASTG 1 cut(s) 105
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 1 cut(s) 443
TspRI CASTG 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 230
XagI CCTNNNNNAGG 1 cut(s) 402
XapI RAATTY 1 cut(s) 190
XmnI GAANNNNTTC 1 cut(s) 434
XspI CTAG 3 cut(s) 203, 326, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.