RchiOBHm_Chr5g0066481

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000618
Physical Location & Seq
Reverse (-)
72640698 .. 72642272
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34225

Sequence Viewer

Length: 1575 bp
ATGGTGCAAATTATTCCACAAAGTGTGGACAAACTATGGAACGAATGGGACGTCCGGGTAATGGTGCTAAGTAGTCTCTGCTTACAAATAGTCCTAATCCTATTGGGAAGCCGGCGAAAGTTCAGTACTCAAAGCTGGGTGAGAATCCTCACATGGACGGCCTACTTATCGGCAGATTGGTTCGCTACAGTCGCGCTTGGAATGATCTCTACATTCCAAGGAGATGGTCTGCAGAACTCACTAACTCCAAACAGCGCTCAAGGAAGTTCCTCAAATCCAAACAGCCCGGCGATCATGGCTTTTTGGGCTCCGTTTCTTCTGGTGCACCTTGGTGGCCCGGACACCATCACTGCTTACTCGTTGGAGGACAACGAGCTGTGGCTAAGGCACCTCTTGGGTTTACTTGTTCAGGTTAGTGCCGCTTTATACATCTTTCTAAGATCATGGACATCCAGCTCCTTACCCCTCTCACTGCTAGCAATTCCAATGTTTGTTGTCGGAATCATCAAATATGGAGAGAGGACTTGGGCCCTCTGGTCTGCTAGCAGTAAGAGACTTAGGGAATCTCTGCCCCCTATTACTGCAGCTCTGACTCCCTTTAAAAATGAGAATCATGAGAGTAGTGGTCGTGTTAGACTAAACTATGCTGCTGCTATTCGTAAAGCCACACATATCCAAGAAGGTCATGCATTATTCAAGTTGTTCAGGCATTTTTACACAGATCAGATCATTGAACAAAAGGATGTTCAACTTTCCGATACTATGATTCAGGACCATTCATTTGGAGATGTCTTCAAGCTAATTGAGATTGAGATTGGTTTTATGTATGATGTGCTGTACACCAAGGCACTCGTAGTGCATTCTCTCACGGGGGTCATGTTCCGAGCCATCTCGCTGGTTTGCTACATTTCCACATTTACGGTCTTTTGTTTCATCATTGATAAGAAGATTTATTTGACCATTGATGTAGATTTGACTTACTTCTTGTGTGCTGGAGCAATTGCACTTGAGATCTATGCCATCTTTGTACAAGTTTCTACAGATTGGACATTGTACTGGTTAAGCAAGCAAGAGCGACCACTGGCGTATACTATCTACCAAGCAATCTCTTCATTTCGATCAAATCTGAGCCTTGAAAAGAGGTGGTCTGAATCCATGGGCCAATTCAATGTCATAGAGTTTTGTTTCAAATTGAAACCGCCAAAGTGTACTGGAATTCCTAGGTTCCTGGCTATCTACGAGATGTATTCTAAACATCAGCACACAGTTTTGAAGGGCGTACCAATCAAAATGAAACAACTTATCTTTGAGCACCTTCAAGATGCTAAACTAGTTGTTGATAATGCAGAGCAGAATTCCAAATTTCAAGTACGTAGTCAAGTCTTGACTTGGAGAGGTGAACATGCGGTACAAAAGAGTGGATGTACTGACAAATATATCAAACATTGTGCCGCGAATGAAGTACCGTTTGAAACCAGCATTGCTTTGTGGCATGTAGCAACAGATATATGCTTCTATAATGATGTTGAGTCAAAAATCAGAAGGCTTCTGATCCTAATTCCAAATCCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

524

Amino Acids

59.86

Weight (kDa)

8.68

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 393 6.5e-94 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 54
AccB1I GGYRCC 1 cut(s) 387
AccI GTMKAC 1 cut(s) 1088
AccII CGCG 2 cut(s) 194, 1454
AciI CCGC 4 cut(s) 420, 1199, 1406, 1452
AclWI GGATC 1 cut(s) 1546
AcsI RAATTY 3 cut(s) 1215, 1354, 1361
AcyI GRCGYC 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 23
AfeI AGCGCT 1 cut(s) 256
AfiI CCNNNNNNNGG 1 cut(s) 61
AhlI ACTAGT 1 cut(s) 1330
AjnI CCWGG 1 cut(s) 1227
AleI CACNNNNGTG 1 cut(s) 330
AluBI AGCT 5 cut(s) 135, 376, 456, 587, 799
AluI AGCT 5 cut(s) 135, 376, 456, 587, 799
Alw21I GWGCWC 2 cut(s) 327, 1314
Alw26I GTCTC 2 cut(s) 80, 547
Alw44I GTGCAC 1 cut(s) 323
AlwI GGATC 1 cut(s) 1546
Aor51HI AGCGCT 1 cut(s) 256
AoxI GGCC 4 cut(s) 159, 334, 528, 1159
ApaI GGGCCC 1 cut(s) 532
ApaLI GTGCAC 1 cut(s) 323
ApeKI GCWGC 3 cut(s) 584, 647, 650
ApoI RAATTY 3 cut(s) 1215, 1354, 1361
ArsI GACNNNNNNTTYG 4 cut(s) 36, 68, 764, 796
AspA2I CCTAGG 1 cut(s) 1220
AspLEI GCGC 2 cut(s) 196, 257
AspS9I GGNCC 5 cut(s) 335, 528, 529, 772, 1159
AsuC2I CCSGG 3 cut(s) 56, 287, 338
AsuHPI GGTGA 2 cut(s) 151, 1409
AsuNHI GCTAGC 2 cut(s) 475, 542
AvaII GGWCC 1 cut(s) 772
AvrII CCTAGG 1 cut(s) 1220
BaeGI GKGCMC 2 cut(s) 327, 532
BanI GGYRCC 1 cut(s) 387
BanII GRGCYC 2 cut(s) 310, 532
BbsI GAAGAC 1 cut(s) 784
Bbv12I GWGCWC 2 cut(s) 327, 1314
BbvI GCAGC 3 cut(s) 596, 634, 637
BccI CCATC 4 cut(s) 218, 353, 896, 1028
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 1 cut(s) 1229
BcnI CCSGG 3 cut(s) 56, 287, 338
BcoDI GTCTC 2 cut(s) 80, 547
BcuI ACTAGT 1 cut(s) 1330
BfaI CTAG 4 cut(s) 476, 543, 1221, 1331
BfmI CTRYAG 4 cut(s) 186, 230, 582, 1038
BfoI RGCGCY 1 cut(s) 258
BglII AGATCT 1 cut(s) 1011
BisI GCNGC 5 cut(s) 420, 585, 648, 651, 1452
BlnI CCTAGG 1 cut(s) 1220
BlsI GCNGC 5 cut(s) 421, 586, 649, 652, 1453
BmcAI AGTACT 1 cut(s) 127
Bme1390I CCNGG 4 cut(s) 56, 287, 338, 1229
Bme18I GGWCC 1 cut(s) 772
BmgT120I GGNCC 5 cut(s) 335, 528, 529, 772, 1159
BmiI GGNNCC 4 cut(s) 309, 389, 530, 1226
BmrFI CCNGG 4 cut(s) 56, 287, 338, 1229
BmsI GCATC 1 cut(s) 1312
BmtI GCTAGC 2 cut(s) 479, 546
BpiI GAAGAC 1 cut(s) 784
BpmI CTGGAG 1 cut(s) 1014
Bpu10I CCTNAGC 1 cut(s) 383
BpuEI CTTGAG 2 cut(s) 243, 1028
BpuMI CCSGG 3 cut(s) 56, 287, 338
BsaAI YACGTR 1 cut(s) 1373
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 6 cut(s) 217, 328, 843, 1155, 1220, 1568
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 3 cut(s) 1061, 1086, 1216
Bse3DI GCAATG 1 cut(s) 1479
BseBI CCWGG 1 cut(s) 1229
BseDI CCNNGG 6 cut(s) 217, 328, 843, 1155, 1220, 1568
BseGI GGATG 3 cut(s) 449, 748, 1427
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 1479
BseMII CTCAG 1 cut(s) 1118
BseNI ACTGG 3 cut(s) 1061, 1086, 1216
BseSI GKGCMC 2 cut(s) 327, 532
BseXI GCAGC 3 cut(s) 596, 634, 637
BseYI CCCAGC 1 cut(s) 135
Bsh1236I CGCG 2 cut(s) 194, 1454
BshFI GGCC 4 cut(s) 161, 336, 530, 1161
BshNI GGYRCC 1 cut(s) 387
BsiHKAI GWGCWC 2 cut(s) 327, 1314
BsiSI CCGG 4 cut(s) 55, 112, 287, 338
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 2 cut(s) 80, 547
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 4 cut(s) 161, 336, 530, 1161
Bsp120I GGGCCC 1 cut(s) 528
Bsp1286I GDGCHC 4 cut(s) 310, 327, 532, 1314
Bsp1407I TGTACA 2 cut(s) 837, 1027
Bsp143I GATC 8 cut(s) 204, 291, 440, 721, 726, 1011, 1118, 1551
Bsp19I CCATGG 1 cut(s) 1155
BspACI CCGC 4 cut(s) 420, 1199, 1406, 1452
BspANI GGCC 4 cut(s) 161, 336, 530, 1161
BspCNI CTCAG 1 cut(s) 1119
BspFNI CGCG 2 cut(s) 194, 1454
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 4 cut(s) 309, 389, 530, 1226
BspMAI CTGCAG 2 cut(s) 234, 586
BspOI GCTAGC 2 cut(s) 479, 546
BspPI GGATC 1 cut(s) 1546
BspT107I GGYRCC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 1479
BsrFI RCCGGY 1 cut(s) 111
BsrGI TGTACA 2 cut(s) 837, 1027
BsrI ACTGG 3 cut(s) 1061, 1086, 1216
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 6 cut(s) 217, 328, 843, 1155, 1220, 1568
BssMI GATC 8 cut(s) 204, 291, 440, 721, 726, 1011, 1118, 1551
BssNAI GTATAC 1 cut(s) 1089
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 6 cut(s) 217, 328, 843, 1155, 1220, 1568
Bst1107I GTATAC 1 cut(s) 1089
Bst2UI CCWGG 1 cut(s) 1229
Bst4CI ACNGT 4 cut(s) 190, 922, 1267, 1467
Bst6I CTCTTC 1 cut(s) 1114
BstACI GRCGYC 1 cut(s) 51
BstAUI TGTACA 2 cut(s) 837, 1027
BstBAI YACGTR 1 cut(s) 1373
BstC8I GCNNGC 4 cut(s) 113, 477, 544, 1067
BstDEI CTNAG 5 cut(s) 68, 383, 437, 557, 1127
BstDSI CCRYGG 1 cut(s) 1155
BstF5I GGATG 3 cut(s) 449, 748, 1427
BstFNI CGCG 2 cut(s) 194, 1454
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 196, 257
BstKTI GATC 8 cut(s) 207, 294, 443, 724, 729, 1014, 1121, 1554
BstMAI GTCTC 2 cut(s) 80, 547
BstMBI GATC 8 cut(s) 204, 291, 440, 721, 726, 1011, 1118, 1551
BstMWI GCNNNNNNNGC 3 cut(s) 191, 296, 305
BstNI CCWGG 1 cut(s) 1229
BstNSI RCATGY 2 cut(s) 1406, 1496
BstSCI CCNGG 4 cut(s) 54, 285, 336, 1227
BstSFI CTRYAG 4 cut(s) 186, 230, 582, 1038
BstSLI GKGCMC 2 cut(s) 327, 532
BstSNI TACGTA 1 cut(s) 1373
BstUI CGCG 2 cut(s) 194, 1454
BstV1I GCAGC 3 cut(s) 596, 634, 637
BstV2I GAAGAC 1 cut(s) 784
BstX2I RGATCY 1 cut(s) 1011
BstXI CCANNNNNNTGG 3 cut(s) 224, 782, 895
BstYI RGATCY 1 cut(s) 1011
BstZ17I GTATAC 1 cut(s) 1089
BsuRI GGCC 4 cut(s) 161, 336, 530, 1161
BtgI CCRYGG 1 cut(s) 1155
BtsCI GGATG 3 cut(s) 449, 748, 1427
BtsI GCAGTG 2 cut(s) 348, 470
BtsIMutI CAGTG 3 cut(s) 348, 470, 1079
Cac8I GCNNGC 4 cut(s) 113, 477, 544, 1067
CciI TCATGA 1 cut(s) 613
CfoI GCGC 2 cut(s) 196, 257
Cfr10I RCCGGY 1 cut(s) 111
Cfr13I GGNCC 5 cut(s) 335, 528, 529, 772, 1159
CviAII CATG 9 cut(s) 153, 295, 444, 614, 686, 877, 1156, 1403, 1493
DdeI CTNAG 5 cut(s) 68, 383, 437, 557, 1127
DpnI GATC 8 cut(s) 206, 293, 442, 723, 728, 1013, 1120, 1553
DpnII GATC 8 cut(s) 204, 291, 440, 721, 726, 1011, 1118, 1551
DraI TTTAAA 1 cut(s) 601
DraIII CACNNNGTG 1 cut(s) 23
Eam1104I CTCTTC 1 cut(s) 1114
EarI CTCTTC 1 cut(s) 1114
Eco105I TACGTA 1 cut(s) 1373
Eco130I CCWWGG 6 cut(s) 217, 328, 843, 1155, 1220, 1568
Eco24I GRGCYC 2 cut(s) 310, 532
Eco47I GGWCC 1 cut(s) 772
Eco47III AGCGCT 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 529
EcoRI GAATTC 2 cut(s) 1215, 1354
EcoRII CCWGG 1 cut(s) 1227
EcoT14I CCWWGG 6 cut(s) 217, 328, 843, 1155, 1220, 1568
EcoT22I ATGCAT 1 cut(s) 691
EcoT38I GRGCYC 2 cut(s) 310, 532
ErhI CCWWGG 6 cut(s) 217, 328, 843, 1155, 1220, 1568
FaeI CATG 9 cut(s) 156, 298, 447, 617, 689, 880, 1159, 1406, 1496
FaqI GGGAC 1 cut(s) 62
FatI CATG 9 cut(s) 152, 294, 443, 613, 685, 876, 1155, 1402, 1492
FblI GTMKAC 1 cut(s) 1088
Fnu4HI GCNGC 5 cut(s) 420, 585, 648, 651, 1452
FokI GGATG 3 cut(s) 436, 755, 1434
FriOI GRGCYC 2 cut(s) 310, 532
Fsp4HI GCNGC 5 cut(s) 420, 585, 648, 651, 1452
FspBI CTAG 4 cut(s) 476, 543, 1221, 1331
GlaI GCGC 2 cut(s) 195, 256
GluI GCNGC 5 cut(s) 420, 585, 648, 651, 1452
GsaI CCCAGC 1 cut(s) 139
GsuI CTGGAG 1 cut(s) 1014
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 4 cut(s) 161, 336, 530, 1161
HapII CCGG 4 cut(s) 55, 112, 287, 338
HhaI GCGC 2 cut(s) 196, 257
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 9 cut(s) 156, 298, 447, 617, 689, 880, 1159, 1406, 1496
Hin6I GCGC 2 cut(s) 194, 255
HinP1I GCGC 2 cut(s) 194, 255
HinfI GANTC 8 cut(s) 144, 501, 563, 592, 610, 766, 1151, 1529
HpaII CCGG 4 cut(s) 55, 112, 287, 338
HphI GGTGA 2 cut(s) 151, 1409
Hpy166II GTNNAC 7 cut(s) 28, 325, 401, 840, 1089, 1209, 1400
Hpy188I TCNGA 9 cut(s) 500, 591, 726, 757, 884, 1128, 1150, 1541, 1551
Hpy188III TCNNGA 4 cut(s) 614, 770, 1319, 1384
Hpy8I GTNNAC 7 cut(s) 28, 325, 401, 840, 1089, 1209, 1400
HpyAV CCTTC 4 cut(s) 674, 1267, 1325, 1536
HpyCH4III ACNGT 4 cut(s) 190, 922, 1267, 1467
HpyCH4IV ACGT 2 cut(s) 51, 1372
HpyCH4V TGCA 8 cut(s) 7, 232, 325, 584, 689, 859, 1004, 1346
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 296, 305
HpyF3I CTNAG 5 cut(s) 68, 383, 437, 557, 1127
HpySE526I ACGT 2 cut(s) 51, 1372
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 9 cut(s) 156, 298, 447, 617, 689, 880, 1159, 1406, 1496
HspAI GCGC 2 cut(s) 194, 255
KroI GCCGGC 1 cut(s) 111
KroNI GCCGGC 1 cut(s) 113
Kzo9I GATC 8 cut(s) 204, 291, 440, 721, 726, 1011, 1118, 1551
LmnI GCTCC 3 cut(s) 313, 461, 995
Lsp1109I GCAGC 3 cut(s) 596, 634, 637
LweI GCATC 1 cut(s) 1312
MaeI CTAG 4 cut(s) 476, 543, 1221, 1331
MaeII ACGT 2 cut(s) 51, 1372
MalI GATC 8 cut(s) 206, 293, 442, 723, 728, 1013, 1120, 1553
MboI GATC 8 cut(s) 204, 291, 440, 721, 726, 1011, 1118, 1551
MboII GAAGA 4 cut(s) 308, 784, 958, 1101
MfeI CAATTG 1 cut(s) 999
MflI RGATCY 1 cut(s) 1011
MhlI GDGCHC 4 cut(s) 310, 327, 532, 1314
MlyI GAGTC 2 cut(s) 586, 1538
MmeI TCCRAC 2 cut(s) 342, 478
MnlI CCTC 9 cut(s) 158, 280, 358, 401, 476, 513, 542, 1134, 1388
Mph1103I ATGCAT 1 cut(s) 691
MroNI GCCGGC 1 cut(s) 111
MseI TTAA 2 cut(s) 600, 1061
MslI CAYNNNNRTG 1 cut(s) 330
MspI CCGG 4 cut(s) 55, 112, 287, 338
MspR9I CCNGG 4 cut(s) 56, 287, 338, 1229
MunI CAATTG 1 cut(s) 999
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 1 cut(s) 1229
MvnI CGCG 2 cut(s) 194, 1454
MwoI GCNNNNNNNGC 3 cut(s) 191, 296, 305
NaeI GCCGGC 1 cut(s) 113
NciI CCSGG 3 cut(s) 56, 287, 338
NcoI CCATGG 1 cut(s) 1155
NdeII GATC 8 cut(s) 204, 291, 440, 721, 726, 1011, 1118, 1551
NgoMIV GCCGGC 1 cut(s) 111
NheI GCTAGC 2 cut(s) 475, 542
NlaIII CATG 9 cut(s) 156, 298, 447, 617, 689, 880, 1159, 1406, 1496
NlaIV GGNNCC 4 cut(s) 309, 389, 530, 1226
NsiI ATGCAT 1 cut(s) 691
NspI RCATGY 2 cut(s) 1406, 1496
OliI CACNNNNGTG 1 cut(s) 330
PagI TCATGA 1 cut(s) 613
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 859
PdiI GCCGGC 1 cut(s) 113
PfeI GAWTC 6 cut(s) 144, 501, 563, 610, 766, 1151
PkrI GCNGC 5 cut(s) 421, 586, 649, 652, 1453
PleI GAGTC 2 cut(s) 586, 1537
PpsI GAGTC 2 cut(s) 586, 1537
Ppu21I YACGTR 1 cut(s) 1373
Psp6I CCWGG 1 cut(s) 1227
PspFI CCCAGC 1 cut(s) 135
PspGI CCWGG 1 cut(s) 1227
PspN4I GGNNCC 4 cut(s) 309, 389, 530, 1226
PspOMI GGGCCC 1 cut(s) 528
PspPI GGNCC 5 cut(s) 335, 528, 529, 772, 1159
PsrI GAACNNNNNNTAC 2 cut(s) 1392, 1424
PstI CTGCAG 2 cut(s) 234, 586
PsuI RGATCY 1 cut(s) 1011
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 2 cut(s) 600, 1061
SatI GCNGC 5 cut(s) 420, 585, 648, 651, 1452
Sau3AI GATC 8 cut(s) 204, 291, 440, 721, 726, 1011, 1118, 1551
Sau96I GGNCC 5 cut(s) 335, 528, 529, 772, 1159
ScaI AGTACT 1 cut(s) 127
SchI GAGTC 2 cut(s) 586, 1538
ScrFI CCNGG 4 cut(s) 56, 287, 338, 1229
SduI GDGCHC 4 cut(s) 310, 327, 532, 1314
SfaNI GCATC 1 cut(s) 1312
SfcI CTRYAG 4 cut(s) 186, 230, 582, 1038
SinI GGWCC 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 2 cut(s) 258, 1007
SmoI CTYRAG 2 cut(s) 258, 1007
SnaBI TACGTA 1 cut(s) 1373
SpeI ACTAGT 1 cut(s) 1330
SsiI CCGC 4 cut(s) 420, 1199, 1406, 1452
SspMI CTAG 4 cut(s) 476, 543, 1221, 1331
StyD4I CCNGG 4 cut(s) 54, 285, 336, 1227
StyI CCWWGG 6 cut(s) 217, 328, 843, 1155, 1220, 1568
TaaI ACNGT 4 cut(s) 190, 922, 1267, 1467
TaiI ACGT 2 cut(s) 54, 1375
TaqI TCGA 1 cut(s) 1117
TatI WGTACW 6 cut(s) 125, 837, 1027, 1053, 1208, 1424
TauI GCSGC 2 cut(s) 422, 1454
TfiI GAWTC 6 cut(s) 144, 501, 563, 610, 766, 1151
Tru1I TTAA 2 cut(s) 600, 1061
Tru9I TTAA 2 cut(s) 600, 1061
TscAI CASTG 3 cut(s) 355, 477, 1086
TseI GCWGC 3 cut(s) 584, 647, 650
TspDTI ATGAA 5 cut(s) 768, 922, 1101, 1307, 1473
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 3 cut(s) 355, 477, 1086
VneI GTGCAC 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 772
XapI RAATTY 3 cut(s) 1215, 1354, 1361
XceI RCATGY 2 cut(s) 1406, 1496
XmaJI CCTAGG 1 cut(s) 1220
XmiI GTMKAC 1 cut(s) 1088
XspI CTAG 4 cut(s) 476, 543, 1221, 1331
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.