Rh2AG446500

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000618
Physical Location & Seq
Forward (+)
65942931 .. 65943779
849 bp
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UTR
Exon/CDS
Intron
Rh2AG446500.1

Sequence Viewer

Length: 849 bp
ATGTTTACACGTTACAATTTACTGAGACCTTTTCTTGAGGAGAAGCCTGCAAAGATCCCTTGGAGATCCAAAATGTCCAAGAATTCAGAGGAGGTTCCTGGAGAGTTGAAAGAACTAATATTTCAGCAGCTTTTGAAGAAATCAAAGAGTGCGACGGAATTTGGTGCTTGCAAGGAACTATGTGCTCGTAGGGGTGATTGGGTTCTTATTAATGAGGGTCTCCATGAGAAACTTCGCTGGAGCATTGAAGAAGAATTCGATAAGAGCATTCTTCTTTGGCATATTGCGACAGACATCTGCTATTATGATGATATGAAGAAGTACTCAAACAAGTTCCATGACTCGAATTGTGAAGACAGCAAGATGTTATCAGAGTATATGTTCTATCTCCTAGTCAAGCGTCCCTTTATGCTTCCCAATGGGATTGCAAAAATCAGGTTGAAAGACACATGTGCTGAAGCCAATAAGTTCTTTGAACAAAGAAATTGCACAAGAAACTACGAGCTGGCTTGCGAGAATTTACTTGAGGTGAGCACTGTAATTCGTCCGGCTGAAATAAAAGGAGACAAAAGCAAGTCGGTGCTATTTGATGCATGCAGACTTGCTCAAGCTTTGCAATCGCTGGAAAAGAAGGAAAAATGGGAGAATACAAAGAAGTGGGAGTTAATGAGTCATGTATGGGTGGAAATGCTATGTTATGCAGCCAGTCAATGTCGATGGAATCATCATGGTCAGCAACTCAGGCGAGGTGGAGAGTTGCTCACTCATGTCTGGTTTCTTATGGCACATCTTGGAATAACTGAACAGCGTGAAAAGGGTCATTCTAGAGCTATGTTTAATGTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

282

Amino Acids

33.42

Weight (kDa)

8.7

Isoelectric Point (pI)

62.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF594 PF04578 213 - 266 3.6e-25 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 49, 60
AcsI RAATTY 4 cut(s) 82, 158, 254, 517
AcuI CTGAAG 1 cut(s) 477
AfaI GTAC 1 cut(s) 323
AflIII ACRYGT 2 cut(s) 8, 449
AgsI TTSAA 5 cut(s) 109, 136, 248, 442, 476
AjnI CCWGG 1 cut(s) 97
AluBI AGCT 4 cut(s) 130, 505, 611, 830
AluI AGCT 4 cut(s) 130, 505, 611, 830
Alw21I GWGCWC 2 cut(s) 187, 536
Alw26I GTCTC 3 cut(s) 19, 224, 558
AlwI GGATC 2 cut(s) 49, 60
ApeKI GCWGC 2 cut(s) 127, 701
ApoI RAATTY 4 cut(s) 82, 158, 254, 517
AseI ATTAAT 1 cut(s) 210
AsuHPI GGTGA 2 cut(s) 206, 541
BbsI GAAGAC 1 cut(s) 360
Bbv12I GWGCWC 2 cut(s) 187, 536
BbvI GCAGC 2 cut(s) 139, 713
BccI CCATC 1 cut(s) 711
BciT130I CCWGG 1 cut(s) 99
BcoDI GTCTC 3 cut(s) 19, 224, 558
BfaI CTAG 2 cut(s) 392, 825
BisI GCNGC 2 cut(s) 128, 702
BlsI GCNGC 2 cut(s) 129, 703
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 1 cut(s) 99
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 1 cut(s) 580
BpiI GAAGAC 1 cut(s) 360
BplI GAGNNNNNCTC 2 cut(s) 744, 776
BpmI CTGGAG 2 cut(s) 120, 259
BpuEI CTTGAG 3 cut(s) 56, 545, 591
BsaI GGTCTC 2 cut(s) 19, 224
BsaJI CCNNGG 1 cut(s) 59
Bse1I ACTGG 1 cut(s) 705
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 59
BseMII CTCAG 2 cut(s) 14, 754
BseNI ACTGG 1 cut(s) 705
BseRI GAGGAG 2 cut(s) 53, 104
BseXI GCAGC 2 cut(s) 139, 713
BsiHKAI GWGCWC 2 cut(s) 187, 536
BsiSI CCGG 1 cut(s) 548
BslFI GGGAC 1 cut(s) 387
BsmAI GTCTC 3 cut(s) 19, 224, 558
BsmFI GGGAC 1 cut(s) 387
BsmI GAATGC 1 cut(s) 267
Bso31I GGTCTC 2 cut(s) 19, 224
Bsp1286I GDGCHC 2 cut(s) 187, 536
Bsp143I GATC 2 cut(s) 54, 65
BspCNI CTCAG 2 cut(s) 15, 753
BspLI GGNNCC 1 cut(s) 96
BspPI GGATC 2 cut(s) 49, 60
BspTNI GGTCTC 2 cut(s) 19, 224
BsrI ACTGG 1 cut(s) 705
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 2 cut(s) 54, 65
BssT1I CCWWGG 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 538
BstC8I GCNNGC 5 cut(s) 48, 169, 507, 511, 595
BstDEI CTNAG 2 cut(s) 23, 740
BstKTI GATC 2 cut(s) 57, 68
BstMAI GTCTC 3 cut(s) 19, 224, 558
BstMBI GATC 2 cut(s) 54, 65
BstMWI GCNNNNNNNGC 1 cut(s) 742
BstNI CCWGG 1 cut(s) 99
BstNSI RCATGY 2 cut(s) 453, 597
BstSCI CCNGG 1 cut(s) 97
BstV1I GCAGC 2 cut(s) 139, 713
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 2 cut(s) 54, 65
BstYI RGATCY 2 cut(s) 54, 65
BtsIMutI CAGTG 1 cut(s) 534
Cac8I GCNNGC 5 cut(s) 48, 169, 507, 511, 595
CseI GACGC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 322
CviAII CATG 7 cut(s) 224, 338, 450, 594, 674, 728, 767
CviJI RGCY 9 cut(s) 46, 130, 461, 505, 509, 551, 611, 704, 830
CviKI_1 RGCY 9 cut(s) 46, 130, 461, 505, 509, 551, 611, 704, 830
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 2 cut(s) 23, 740
DpnI GATC 2 cut(s) 56, 67
DpnII GATC 2 cut(s) 54, 65
Eco130I CCWWGG 1 cut(s) 59
Eco31I GGTCTC 2 cut(s) 19, 224
Eco57I CTGAAG 1 cut(s) 477
EcoRI GAATTC 2 cut(s) 82, 254
EcoRII CCWGG 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 59
EcoT22I ATGCAT 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 59
FaeI CATG 7 cut(s) 227, 341, 453, 597, 677, 731, 770
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FaqI GGGAC 1 cut(s) 387
FatI CATG 7 cut(s) 223, 337, 449, 593, 673, 727, 766
Fnu4HI GCNGC 2 cut(s) 128, 702
Fsp4HI GCNGC 2 cut(s) 128, 702
FspBI CTAG 2 cut(s) 392, 825
GluI GCNGC 2 cut(s) 128, 702
GsuI CTGGAG 2 cut(s) 120, 259
HapII CCGG 1 cut(s) 548
HgaI GACGC 1 cut(s) 389
Hin1II CATG 7 cut(s) 227, 341, 453, 597, 677, 731, 770
HindIII AAGCTT 1 cut(s) 609
HinfI GANTC 3 cut(s) 341, 670, 721
HpaII CCGG 1 cut(s) 548
HphI GGTGA 2 cut(s) 206, 541
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 88, 373
Hpy188III TCNNGA 2 cut(s) 35, 825
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 1 cut(s) 625
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 8 cut(s) 50, 171, 428, 489, 593, 597, 616, 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 742
HpyF3I CTNAG 2 cut(s) 23, 740
HpySE526I ACGT 1 cut(s) 10
Hsp92II CATG 7 cut(s) 227, 341, 453, 597, 677, 731, 770
Kzo9I GATC 2 cut(s) 54, 65
LmnI GCTCC 1 cut(s) 240
Lsp1109I GCAGC 2 cut(s) 139, 713
LweI GCATC 1 cut(s) 580
MaeI CTAG 2 cut(s) 392, 825
MaeII ACGT 1 cut(s) 10
MaeIII GTNAC 1 cut(s) 11
MalI GATC 2 cut(s) 56, 67
MboI GATC 2 cut(s) 54, 65
MboII GAAGA 6 cut(s) 148, 260, 263, 263, 328, 365
MflI RGATCY 2 cut(s) 54, 65
MhlI GDGCHC 2 cut(s) 187, 536
MluCI AATT 8 cut(s) 16, 82, 158, 254, 346, 484, 517, 540
MlyI GAGTC 2 cut(s) 335, 679
MnlI CCTC 6 cut(s) 31, 82, 85, 208, 520, 740
Mph1103I ATGCAT 1 cut(s) 595
MseI TTAA 3 cut(s) 210, 665, 837
MspI CCGG 1 cut(s) 548
MspR9I CCNGG 1 cut(s) 99
Mva1269I GAATGC 1 cut(s) 267
MvaI CCWGG 1 cut(s) 99
MwoI GCNNNNNNNGC 1 cut(s) 742
NdeII GATC 2 cut(s) 54, 65
NlaIII CATG 7 cut(s) 227, 341, 453, 597, 677, 731, 770
NlaIV GGNNCC 1 cut(s) 96
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 2 cut(s) 453, 597
PaeI GCATGC 1 cut(s) 597
PciI ACATGT 1 cut(s) 449
PctI GAATGC 1 cut(s) 267
PfeI GAWTC 1 cut(s) 721
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 2 cut(s) 129, 703
PleI GAGTC 2 cut(s) 335, 678
PpsI GAGTC 2 cut(s) 335, 678
PscI ACATGT 1 cut(s) 449
PshBI ATTAAT 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 96
PsuI RGATCY 2 cut(s) 54, 65
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SaqAI TTAA 3 cut(s) 210, 665, 837
SatI GCNGC 2 cut(s) 128, 702
Sau3AI GATC 2 cut(s) 54, 65
ScaI AGTACT 1 cut(s) 323
SchI GAGTC 2 cut(s) 335, 679
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 2 cut(s) 187, 536
SfaNI GCATC 1 cut(s) 580
SmlI CTYRAG 3 cut(s) 35, 524, 606
SmoI CTYRAG 3 cut(s) 35, 524, 606
SphI GCATGC 1 cut(s) 597
Sse9I AATT 8 cut(s) 16, 82, 158, 254, 346, 484, 517, 540
SspI AATATT 1 cut(s) 120
SspMI CTAG 2 cut(s) 392, 825
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 1 cut(s) 538
TaiI ACGT 1 cut(s) 13
TaqI TCGA 3 cut(s) 258, 344, 715
TasI AATT 8 cut(s) 16, 82, 158, 254, 346, 484, 517, 540
TatI WGTACW 1 cut(s) 321
TfiI GAWTC 1 cut(s) 721
Tru1I TTAA 3 cut(s) 210, 665, 837
Tru9I TTAA 3 cut(s) 210, 665, 837
TscAI CASTG 1 cut(s) 541
TseI GCWGC 2 cut(s) 127, 701
TspDTI ATGAA 1 cut(s) 329
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 1 cut(s) 541
VspI ATTAAT 1 cut(s) 210
XapI RAATTY 4 cut(s) 82, 158, 254, 517
XbaI TCTAGA 1 cut(s) 824
XceI RCATGY 2 cut(s) 453, 597
XspI CTAG 2 cut(s) 392, 825
ZrmI AGTACT 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.