Rw5G040940

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000618
Physical Location & Seq
Reverse (-)
72276060 .. 72283759
7700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G040940.1

Sequence Viewer

Length: 1092 bp
ATGAGGAATATGTTGCAAATTATTCCAGAAAATTTGGACAAACTATGGAACGAATGTGATGTCCGGATAATTTGTAACCGAAGAAAATTCCGAAACTCGATGAGAATTATCACATGGATAGCCTACTTATCAGCGGATTGGGTTGCTACTGTCGCACTTGGAGTGATCATCACTGCTTATTCACTTGAAGGCAATGAGCTCTGGTTAAGGCACCTCTTAGGCTTACTAGTCCAAGTTACACTAGCACATACCAGCTCTCACTTCTATCATGGAATTATCAAGTATAGAGAGAGGACTTGGGTCCTGCAGTCTACAACCAGTGATAGATATAGAAACTTGTTGGTACCTAATCCTAATCATGGTAATGCTCATCTTGGTTCCCGCAATGATGCCGAGGAAACAGATGTAGAGGATCTAAGAAACAGGTTAAGTTTTTGGAGAATAAGTTCCAAAGATTTGGTAGCGGGGACAAAACCTAGTACTTTAGAAGCCACATTAATTCATCAAGCCTATGTGATTTATCCTTTGATTGTGGGCAGTTCATCAGAAGATTACTTCGAAGTAGTAGAGATTGAGCTTGGTTTTATGTATGATTTGCTGTACACAAAGGCAACCATGATATACTCTTTCAAAGGTATCATTTTCTGCTCCATCTGCATGCTTTCTATTACTCTTGCACTATATGCCATTGCCGTACTACTTTCCATAGACTGGAAACTATACTGGTTAAGCAGGCAAAAGAATATGCTTGCCTATTCCATCTTCAAAGGAATCAGTTGCTTTAGGTCAATTTTAAGTCATCGCAAGAGGTGGTCTGAATCCATGGCACAATTTAGGCCCAATTTGGTATTGCTTTTGGAACCTGCTTTTGGTCCAAAAAGAAACCCCTCAAGGCTCTTTGGTATCTTCGATACGTATGAAAAGTATCGACATGCGCCTTTTGCAAAAGTCTCTGTCAGAATGAAAGAGCTTATCTTTCAGAAACTTCAAGATGCTAGAAATCATGTTAATGAAGAAAGAGGTCCTGAACTAAAATATGATCAAATTAGGGATGTCTTGACTTGGAAAGGGAACCATGCGTTACAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

363

Amino Acids

42.29

Weight (kDa)

9.29

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 57 - 233 4.4e-28 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 871
Acc65I GGTACC 1 cut(s) 343
AccB1I GGYRCC 2 cut(s) 210, 343
AccB7I CCANNNNNTGG 1 cut(s) 711
AccI GTMKAC 1 cut(s) 311
AccIII TCCGGA 1 cut(s) 63
AciI CCGC 3 cut(s) 134, 382, 464
AclWI GGATC 1 cut(s) 420
AcsI RAATTY 2 cut(s) 31, 86
AfaI GTAC 4 cut(s) 345, 481, 602, 696
AfiI CCNNNNNNNGG 3 cut(s) 359, 711, 869
AgsI TTSAA 4 cut(s) 188, 631, 766, 989
AhlI ACTAGT 1 cut(s) 226
AjuI GAANNNNNNNTTGG 2 cut(s) 852, 884
AloI GAACNNNNNNTCC 2 cut(s) 430, 462
AluBI AGCT 4 cut(s) 199, 255, 577, 970
AluI AGCT 4 cut(s) 199, 255, 577, 970
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 1 cut(s) 955
AlwI GGATC 1 cut(s) 420
Aor13HI TCCGGA 1 cut(s) 63
AoxI GGCC 1 cut(s) 836
ApoI RAATTY 2 cut(s) 31, 86
ArsI GACNNNNNNTTYG 2 cut(s) 45, 77
AseI ATTAAT 1 cut(s) 497
Asp700I GAANNNNTTC 1 cut(s) 445
Asp718I GGTACC 1 cut(s) 343
AspLEI GCGC 1 cut(s) 937
AspS9I GGNCC 4 cut(s) 301, 837, 872, 1022
AsuII TTCGAA 1 cut(s) 558
AvaII GGWCC 3 cut(s) 301, 872, 1022
BanI GGYRCC 2 cut(s) 210, 343
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 201
BccI CCATC 2 cut(s) 659, 767
BceAI ACGGC 1 cut(s) 677
BclI TGATCA 2 cut(s) 165, 1039
BcoDI GTCTC 1 cut(s) 955
BcuI ACTAGT 1 cut(s) 226
BfaI CTAG 4 cut(s) 227, 242, 477, 996
BfmI CTRYAG 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 871
BmcAI AGTACT 1 cut(s) 481
Bme18I GGWCC 3 cut(s) 301, 872, 1022
BmgT120I GGNCC 4 cut(s) 301, 837, 872, 1022
BmiI GGNNCC 6 cut(s) 212, 302, 345, 379, 861, 1073
BmsI GCATC 2 cut(s) 379, 982
BoxI GACNNNNGTC 1 cut(s) 299
Bpu14I TTCGAA 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 874
BsaAI YACGTR 1 cut(s) 915
BsaJI CCNNGG 2 cut(s) 393, 822
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 430, 460
Bsc4I CCNNNNNNNGG 3 cut(s) 359, 711, 869
Bse1I ACTGG 3 cut(s) 318, 716, 728
Bse3DI GCAATG 3 cut(s) 199, 391, 687
BseAI TCCGGA 1 cut(s) 63
BseDI CCNNGG 2 cut(s) 393, 822
BseGI GGATG 1 cut(s) 1057
BseLI CCNNNNNNNGG 3 cut(s) 359, 711, 869
BseMI GCAATG 3 cut(s) 199, 391, 687
BseNI ACTGG 3 cut(s) 318, 716, 728
BshFI GGCC 1 cut(s) 838
BshNI GGYRCC 2 cut(s) 210, 343
BsiHKAI GWGCWC 1 cut(s) 201
BsiSI CCGG 1 cut(s) 64
BslFI GGGAC 1 cut(s) 481
BslI CCNNNNNNNGG 3 cut(s) 359, 711, 869
BsmAI GTCTC 1 cut(s) 955
BsmFI GGGAC 1 cut(s) 481
BsnI GGCC 1 cut(s) 838
Bsp119I TTCGAA 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 201
Bsp13I TCCGGA 1 cut(s) 63
Bsp1407I TGTACA 1 cut(s) 600
Bsp143I GATC 3 cut(s) 165, 412, 1039
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 3 cut(s) 134, 382, 464
BspANI GGCC 1 cut(s) 838
BspEI TCCGGA 1 cut(s) 63
BspLI GGNNCC 6 cut(s) 212, 302, 345, 379, 861, 1073
BspMAI CTGCAG 1 cut(s) 309
BspMI ACCTGC 1 cut(s) 871
BspPI GGATC 1 cut(s) 420
BspT104I TTCGAA 1 cut(s) 558
BspT107I GGYRCC 2 cut(s) 210, 343
BsrDI GCAATG 3 cut(s) 199, 391, 687
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 3 cut(s) 318, 716, 728
BssECI CCNNGG 2 cut(s) 393, 822
BssMI GATC 3 cut(s) 165, 412, 1039
BssT1I CCWWGG 1 cut(s) 822
Bst4CI ACNGT 1 cut(s) 151
BstAPI GCANNNNNTGC 1 cut(s) 683
BstAUI TGTACA 1 cut(s) 600
BstBAI YACGTR 1 cut(s) 915
BstBI TTCGAA 1 cut(s) 558
BstC8I GCNNGC 3 cut(s) 659, 734, 750
BstDEI CTNAG 2 cut(s) 217, 416
BstDSI CCRYGG 1 cut(s) 822
BstF5I GGATG 1 cut(s) 1057
BstHHI GCGC 1 cut(s) 937
BstKTI GATC 3 cut(s) 168, 415, 1042
BstMAI GTCTC 1 cut(s) 955
BstMBI GATC 3 cut(s) 165, 412, 1039
BstMWI GCNNNNNNNGC 4 cut(s) 152, 654, 683, 941
BstNSI RCATGY 2 cut(s) 661, 935
BstPAI GACNNNNGTC 1 cut(s) 299
BstSFI CTRYAG 1 cut(s) 305
BstSNI TACGTA 1 cut(s) 915
BstX2I RGATCY 1 cut(s) 412
BstXI CCANNNNNNTGG 1 cut(s) 457
BstYI RGATCY 1 cut(s) 412
BsuRI GGCC 1 cut(s) 838
BtgI CCRYGG 1 cut(s) 822
BtgZI GCGATG 1 cut(s) 785
BtsCI GGATG 1 cut(s) 1057
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 171, 325
BveI ACCTGC 1 cut(s) 871
Cac8I GCNNGC 3 cut(s) 659, 734, 750
CfoI GCGC 1 cut(s) 937
Cfr13I GGNCC 4 cut(s) 301, 837, 872, 1022
Csp6I GTAC 4 cut(s) 344, 480, 601, 695
CviAII CATG 9 cut(s) 114, 269, 359, 616, 658, 823, 932, 1004, 1076
CviQI GTAC 4 cut(s) 344, 480, 601, 695
DdeI CTNAG 2 cut(s) 217, 416
DpnI GATC 3 cut(s) 167, 414, 1041
DpnII GATC 3 cut(s) 165, 412, 1039
Ecl136II GAGCTC 1 cut(s) 199
Eco105I TACGTA 1 cut(s) 915
Eco130I CCWWGG 1 cut(s) 822
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 3 cut(s) 301, 872, 1022
Eco53kI GAGCTC 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 199
EcoO109I RGGNCCY 2 cut(s) 301, 1022
EcoT14I CCWWGG 1 cut(s) 822
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 822
FaeI CATG 9 cut(s) 117, 272, 362, 619, 661, 826, 935, 1007, 1079
FaqI GGGAC 1 cut(s) 481
FatI CATG 9 cut(s) 113, 268, 358, 615, 657, 822, 931, 1003, 1075
FauI CCCGC 2 cut(s) 389, 457
FbaI TGATCA 2 cut(s) 165, 1039
FblI GTMKAC 1 cut(s) 311
FokI GGATG 1 cut(s) 1064
FriOI GRGCYC 1 cut(s) 201
FspBI CTAG 4 cut(s) 227, 242, 477, 996
GlaI GCGC 1 cut(s) 936
HaeIII GGCC 1 cut(s) 838
HapII CCGG 1 cut(s) 64
HhaI GCGC 1 cut(s) 937
Hin1II CATG 9 cut(s) 117, 272, 362, 619, 661, 826, 935, 1007, 1079
Hin6I GCGC 1 cut(s) 935
HinP1I GCGC 1 cut(s) 935
HinfI GANTC 2 cut(s) 771, 818
HpaII CCGG 1 cut(s) 64
Hpy166II GTNNAC 2 cut(s) 312, 603
Hpy188I TCNGA 5 cut(s) 92, 547, 817, 959, 981
Hpy188III TCNNGA 5 cut(s) 26, 64, 989, 1025, 1057
Hpy8I GTNNAC 2 cut(s) 312, 603
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 1 cut(s) 914
HpyCH4V TGCA 5 cut(s) 16, 307, 657, 677, 944
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 654, 683, 941
HpyF3I CTNAG 2 cut(s) 217, 416
HpySE526I ACGT 1 cut(s) 914
Hsp92II CATG 9 cut(s) 117, 272, 362, 619, 661, 826, 935, 1007, 1079
HspAI GCGC 1 cut(s) 935
Kpn2I TCCGGA 1 cut(s) 63
KpnI GGTACC 1 cut(s) 347
Ksp22I TGATCA 2 cut(s) 165, 1039
Kzo9I GATC 3 cut(s) 165, 412, 1039
LmnI GCTCC 1 cut(s) 653
LweI GCATC 2 cut(s) 379, 982
MaeI CTAG 4 cut(s) 227, 242, 477, 996
MaeII ACGT 1 cut(s) 914
MaeIII GTNAC 3 cut(s) 74, 235, 1080
MalI GATC 3 cut(s) 167, 414, 1041
MboI GATC 3 cut(s) 165, 412, 1039
MboII GAAGA 5 cut(s) 93, 560, 754, 898, 1025
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 1 cut(s) 201
MnlI CCTC 7 cut(s) 224, 285, 388, 403, 801, 898, 1013
MroI TCCGGA 1 cut(s) 63
MroXI GAANNNNTTC 1 cut(s) 445
MseI TTAA 6 cut(s) 206, 428, 497, 728, 794, 1008
MslI CAYNNNNRTG 3 cut(s) 363, 656, 1008
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 1 cut(s) 64
MwoI GCNNNNNNNGC 4 cut(s) 152, 654, 683, 941
NcoI CCATGG 1 cut(s) 822
NdeII GATC 3 cut(s) 165, 412, 1039
NlaIII CATG 9 cut(s) 117, 272, 362, 619, 661, 826, 935, 1007, 1079
NlaIV GGNNCC 6 cut(s) 212, 302, 345, 379, 861, 1073
NmeAIII GCCGAG 1 cut(s) 418
NspI RCATGY 2 cut(s) 661, 935
NspV TTCGAA 1 cut(s) 558
PaeI GCATGC 1 cut(s) 661
PdmI GAANNNNTTC 1 cut(s) 445
PfeI GAWTC 2 cut(s) 771, 818
PflMI CCANNNNNTGG 1 cut(s) 711
Ppu21I YACGTR 1 cut(s) 915
PpuMI RGGWCCY 2 cut(s) 301, 1022
PshAI GACNNNNGTC 1 cut(s) 299
PshBI ATTAAT 1 cut(s) 497
Psp124BI GAGCTC 1 cut(s) 201
Psp5II RGGWCCY 2 cut(s) 301, 1022
PspN4I GGNNCC 6 cut(s) 212, 302, 345, 379, 861, 1073
PspPI GGNCC 4 cut(s) 301, 837, 872, 1022
PspPPI RGGWCCY 2 cut(s) 301, 1022
PstI CTGCAG 1 cut(s) 309
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 4 cut(s) 345, 481, 602, 696
RsaNI GTAC 4 cut(s) 344, 480, 601, 695
RseI CAYNNNNRTG 3 cut(s) 363, 656, 1008
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 6 cut(s) 206, 428, 497, 728, 794, 1008
Sau3AI GATC 3 cut(s) 165, 412, 1039
Sau96I GGNCC 4 cut(s) 301, 837, 872, 1022
ScaI AGTACT 1 cut(s) 481
SduI GDGCHC 1 cut(s) 201
SfaNI GCATC 2 cut(s) 379, 982
SfcI CTRYAG 1 cut(s) 305
SfuI TTCGAA 1 cut(s) 558
SinI GGWCC 3 cut(s) 301, 872, 1022
SmiMI CAYNNNNRTG 3 cut(s) 363, 656, 1008
SmlI CTYRAG 1 cut(s) 889
SmoI CTYRAG 1 cut(s) 889
SnaBI TACGTA 1 cut(s) 915
SpeI ACTAGT 1 cut(s) 226
SphI GCATGC 1 cut(s) 661
SsiI CCGC 3 cut(s) 134, 382, 464
SspMI CTAG 4 cut(s) 227, 242, 477, 996
SstI GAGCTC 1 cut(s) 201
StyI CCWWGG 1 cut(s) 822
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 1 cut(s) 917
TaqI TCGA 4 cut(s) 98, 558, 909, 928
TatI WGTACW 2 cut(s) 479, 600
TfiI GAWTC 2 cut(s) 771, 818
Tru1I TTAA 6 cut(s) 206, 428, 497, 728, 794, 1008
Tru9I TTAA 6 cut(s) 206, 428, 497, 728, 794, 1008
TscAI CASTG 2 cut(s) 178, 325
TspDTI ATGAA 5 cut(s) 491, 531, 933, 977, 1026
TspRI CASTG 2 cut(s) 178, 325
Van91I CCANNNNNTGG 1 cut(s) 711
VpaK11BI GGWCC 3 cut(s) 301, 872, 1022
VspI ATTAAT 1 cut(s) 497
XapI RAATTY 2 cut(s) 31, 86
XceI RCATGY 2 cut(s) 661, 935
XmiI GTMKAC 1 cut(s) 311
XmnI GAANNNNTTC 1 cut(s) 445
XspI CTAG 4 cut(s) 227, 242, 477, 996
ZrmI AGTACT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.