Rroxscaffold_1G00014400

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000618
Physical Location & Seq
Forward (+)
17790913 .. 17793033
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00014400.1

Sequence Viewer

Length: 2121 bp
ATGGTGCAAATTATTCCACAAAGTGTGGACAAACTATGGAACGAATGGGACGTCCGGGTAATGGTGCTAAGTAGTCTCTGCTTACAAATAGTCCTAATCCTCTTGGGAAGCCGGCGAAAGTTCAGTACTCAAAGCTGGGTGAGAATCCTCACATGGACGGCCTACTTATCGGCAGATTGGTTCGCTACCGTCGCGCTTGGAATGATCTCCACGTTCCAAGGAGATGGTCAACAAAACTCACTAACTCCAAACAGCGCTCAAGGAAGTTCCTCAAATCCAAACAGCCCGGCGATCATGGCTATTTGGGCTCCGTTTCTTCTGGTGCACCTTGGTGGCCCGGACACCATAACTGCTTACTCGTTGGAGGACAACGAGCTGTGGCTAAGGCACCTCTTGGGTTTACTTGTTCAGGTTAGTGCCGCTTTATACATCTTTCTAAGATCATGGACATCAAGCTCCTTACCCCTCTCACTGCTAGCAATACCAATGTTTGTTGTCGGAATCATCAAATATGGAGAGAGGACTTGGGCCCTCTGGTCTGCTAGCAGTGAGAGACTTAGGGAATCTCTGCCCCCTATTACTGCAGCTCTGACTCCCTTTAAAAATGAGAATCATGAGAGTAGTGGTCGTGTTAGCTATGCTGCTGCTATTCGTAATGCCACACATATCCAAGAAGGTCATGCATTATTCAAGTTGTTCAGGCATTTTTACACAGATCAGATCATTGAACAAAAGGATGTTCAACTTTCCAATACTATGATTCAGAACCATTCATTTGGAGATGTCTTCAAGCTAATTGAGATTGAGATTGGTTTTATGTATGATGTGCTGTACACCAAGGCAATCGTAGTGCATTCTCTCACGGGGGTCATGTTCCGAGCCATCTCGCTGGTTTGCTACATTTCCACATTTACGGTCTTTTGTTTCATCATTGATAAGAAGATTTATTCGCCCATTGATGTAGATTTGACTTACTTCTTGTGTGCTGGAGCAATTGCACTTGAGATCTATGCCATCTTTGTACAAGTTTCTACAGATTGGACATTGTACTGGTTAAGCAAGCAAGAGCGACCGCTGGCGTATACTATCTACCAAGCAATCTCTTCATTTCGATTGAATCTGAGCCTTGAAAAGAGGTGGTCTGAATCCATGGGCCAATTCAATGTCATAGAGTTTTGTTTCAAATTGAAACCGCCAAAGTGTAGTGGAATTCCTAGGTTCCTGGCTGTCTACGAGATGTATTCTAAACATCAGCACACAGTTTTGGAGGGCGTACCAATCAAAATGAAACAACTTATCTTTGAGCACCTTCAAGATGCTAAACTAGTTGTTGATAATGCAGAGCAGAATTCCAAATTTCAAGTACGTAGTCAAGTCTTGACTTGGAGAGGTGAACATGCGGTGGAAAAGAGTGGATGTACTGACAAATATATCAAACATTGTGCCGCGAATGAAGTACCGTTTGAAACCAGCATTGCTTTGTGGCATGTAGCAACAGATATATGCTTCTATAATGATGTTCACAAAAATCAAAAGGCCTCTGATCCTAATTCCAAATCCAAGGTGAGCAAGTTGCTGTCAGATTATATGATGTATATTCTTGCCATCTGCCCTTTCATGTTGCCCCAAGGGATTGGACAGATTTTGTTTCGACAAATATGTGAGTCTGCCACAACATTTTTCGATGAGAGAAGGGAAACCATAAGTAAGACAGAGCGATTGGTTGGAAAAAGTGCCAGTCAGGTGTTGTACGAGATGAAGTCAGAGTTGAATCCATGGCTAGATGAGTTTGAGTCCGGAGATGGAGGAAAACTAAACAATGTAAGGTTGCTTGAAGGGTGTGTGCTTGCACATAAGTTGCAGGCCCTGGAAACGGAGGAGAGCTGGACTAATGAAGATAAATGGGGAATGATAAGTCGAGTGTGGATAGAAATGTTGTGTTATGCAGCAAGCCAATGTGGATGGACGGAGCACGGTCAACAACTTAGGCGTGGCGGAGAGCTTCTTACTCATGTCTGCCTTCTCATGGCAAATCTTGGTATTGGTGAACAGAGTAAGATATTGAGTGAGCAGGACATGGACCGGTTACAGGATTTAAAAAACTTGTGTGAAAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

706

Amino Acids

80.42

Weight (kDa)

6.25

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 391 2.6e-94 Domain of unknown function (DUF4220)
DUF594 PF04578 629 - 681 1.3e-21 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 54
AccB1I GGYRCC 1 cut(s) 387
AccI GTMKAC 2 cut(s) 1082, 1230
AccII CGCG 2 cut(s) 194, 1448
AccIII TCCGGA 1 cut(s) 1796
AciI CCGC 6 cut(s) 420, 1073, 1193, 1400, 1446, 1995
AclWI GGATC 1 cut(s) 1538
AcsI RAATTY 3 cut(s) 1209, 1348, 1355
AcyI GRCGYC 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 23
AfaI GTAC 9 cut(s) 127, 833, 1023, 1049, 1275, 1365, 1420, 1458, 1751
AfeI AGCGCT 1 cut(s) 256
AfiI CCNNNNNNNGG 4 cut(s) 61, 1873, 2026, 2089
AgeI ACCGGT 1 cut(s) 2082
AhlI ACTAGT 1 cut(s) 1324
AjnI CCWGG 2 cut(s) 1221, 1866
AleI CACNNNNGTG 1 cut(s) 330
AluBI AGCT 8 cut(s) 135, 376, 456, 587, 636, 793, 1884, 2002
AluI AGCT 8 cut(s) 135, 376, 456, 587, 636, 793, 1884, 2002
Alw21I GWGCWC 3 cut(s) 327, 1308, 1974
Alw26I GTCTC 2 cut(s) 80, 547
Alw44I GTGCAC 1 cut(s) 323
AlwI GGATC 1 cut(s) 1538
AlwNI CAGNNNCTG 1 cut(s) 1867
Aor13HI TCCGGA 1 cut(s) 1796
Aor51HI AGCGCT 1 cut(s) 256
AoxI GGCC 6 cut(s) 159, 334, 528, 1153, 1536, 1863
ApaI GGGCCC 1 cut(s) 532
ApaLI GTGCAC 1 cut(s) 323
ApeKI GCWGC 4 cut(s) 584, 641, 644, 1946
ApoI RAATTY 3 cut(s) 1209, 1348, 1355
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AsiGI ACCGGT 1 cut(s) 2082
AspA2I CCTAGG 1 cut(s) 1214
AspLEI GCGC 2 cut(s) 196, 257
AspS9I GGNCC 6 cut(s) 335, 528, 529, 1153, 1864, 2080
AsuC2I CCSGG 3 cut(s) 56, 287, 338
AsuHPI GGTGA 4 cut(s) 151, 1403, 1576, 2057
AsuNHI GCTAGC 2 cut(s) 475, 542
AvaII GGWCC 1 cut(s) 2080
AvrII CCTAGG 1 cut(s) 1214
BaeGI GKGCMC 2 cut(s) 327, 532
BanI GGYRCC 1 cut(s) 387
BanII GRGCYC 2 cut(s) 310, 532
BbsI GAAGAC 1 cut(s) 778
Bbv12I GWGCWC 3 cut(s) 327, 1308, 1974
BbvI GCAGC 4 cut(s) 596, 628, 631, 1958
BccI CCATC 6 cut(s) 218, 890, 1022, 1613, 1796, 1956
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 2 cut(s) 1223, 1868
BcnI CCSGG 3 cut(s) 56, 287, 338
BcoDI GTCTC 2 cut(s) 80, 547
BcuI ACTAGT 1 cut(s) 1324
BfaI CTAG 5 cut(s) 476, 543, 1215, 1325, 1781
BfmI CTRYAG 2 cut(s) 582, 1032
BfoI RGCGCY 1 cut(s) 258
BglII AGATCT 1 cut(s) 1005
BisI GCNGC 6 cut(s) 420, 585, 642, 645, 1446, 1947
BlnI CCTAGG 1 cut(s) 1214
BlsI GCNGC 6 cut(s) 421, 586, 643, 646, 1447, 1948
BmcAI AGTACT 1 cut(s) 127
Bme1390I CCNGG 5 cut(s) 56, 287, 338, 1223, 1868
Bme18I GGWCC 1 cut(s) 2080
BmgT120I GGNCC 6 cut(s) 335, 528, 529, 1153, 1864, 2080
BmiI GGNNCC 4 cut(s) 309, 389, 530, 1220
BmrFI CCNGG 5 cut(s) 56, 287, 338, 1223, 1868
BmsI GCATC 1 cut(s) 1306
BmtI GCTAGC 2 cut(s) 479, 546
BpiI GAAGAC 1 cut(s) 778
BpmI CTGGAG 1 cut(s) 1008
Bpu10I CCTNAGC 1 cut(s) 383
BpuEI CTTGAG 2 cut(s) 243, 1022
BpuMI CCSGG 3 cut(s) 56, 287, 338
BsaAI YACGTR 1 cut(s) 1367
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 9 cut(s) 217, 328, 837, 1149, 1214, 1560, 1627, 1775, 1866
BsaWI WCCGGW 2 cut(s) 1796, 2082
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 1873, 2026, 2089
Bse118I RCCGGY 2 cut(s) 111, 2082
Bse1I ACTGG 2 cut(s) 1055, 1737
Bse3DI GCAATG 1 cut(s) 1473
BseAI TCCGGA 1 cut(s) 1796
BseBI CCWGG 2 cut(s) 1223, 1868
BseDI CCNNGG 9 cut(s) 217, 328, 837, 1149, 1214, 1560, 1627, 1775, 1866
BseGI GGATG 3 cut(s) 742, 1421, 1967
BseLI CCNNNNNNNGG 4 cut(s) 61, 1873, 2026, 2089
BseMI GCAATG 1 cut(s) 1473
BseMII CTCAG 1 cut(s) 1112
BseNI ACTGG 2 cut(s) 1055, 1737
BseRI GAGGAG 1 cut(s) 1892
BseSI GKGCMC 2 cut(s) 327, 532
BseXI GCAGC 4 cut(s) 596, 628, 631, 1958
BseYI CCCAGC 1 cut(s) 135
Bsh1236I CGCG 2 cut(s) 194, 1448
Bsh1285I CGRYCG 1 cut(s) 1073
BshFI GGCC 6 cut(s) 161, 336, 530, 1155, 1538, 1865
BshNI GGYRCC 1 cut(s) 387
BshTI ACCGGT 1 cut(s) 2082
BsiEI CGRYCG 1 cut(s) 1073
BsiHKAI GWGCWC 3 cut(s) 327, 1308, 1974
BsiSI CCGG 6 cut(s) 55, 112, 287, 338, 1797, 2083
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 4 cut(s) 61, 1873, 2026, 2089
BsmAI GTCTC 2 cut(s) 80, 547
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 6 cut(s) 161, 336, 530, 1155, 1538, 1865
Bsp120I GGGCCC 1 cut(s) 528
Bsp1286I GDGCHC 5 cut(s) 310, 327, 532, 1308, 1974
Bsp13I TCCGGA 1 cut(s) 1796
Bsp1407I TGTACA 2 cut(s) 831, 1021
Bsp143I GATC 7 cut(s) 204, 291, 440, 715, 720, 1005, 1543
Bsp19I CCATGG 2 cut(s) 1149, 1775
BspACI CCGC 6 cut(s) 420, 1073, 1193, 1400, 1446, 1995
BspANI GGCC 6 cut(s) 161, 336, 530, 1155, 1538, 1865
BspCNI CTCAG 1 cut(s) 1113
BspEI TCCGGA 1 cut(s) 1796
BspFNI CGCG 2 cut(s) 194, 1448
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 4 cut(s) 309, 389, 530, 1220
BspMAI CTGCAG 1 cut(s) 586
BspOI GCTAGC 2 cut(s) 479, 546
BspPI GGATC 1 cut(s) 1538
BspT107I GGYRCC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 1473
BsrFI RCCGGY 2 cut(s) 111, 2082
BsrGI TGTACA 2 cut(s) 831, 1021
BsrI ACTGG 2 cut(s) 1055, 1737
BssAI RCCGGY 2 cut(s) 111, 2082
BssECI CCNNGG 9 cut(s) 217, 328, 837, 1149, 1214, 1560, 1627, 1775, 1866
BssMI GATC 7 cut(s) 204, 291, 440, 715, 720, 1005, 1543
BssNAI GTATAC 1 cut(s) 1083
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 8 cut(s) 217, 328, 837, 1149, 1214, 1560, 1627, 1775
Bst1107I GTATAC 1 cut(s) 1083
Bst2UI CCWGG 2 cut(s) 1223, 1868
Bst4CI ACNGT 5 cut(s) 190, 916, 1261, 1461, 1976
Bst6I CTCTTC 1 cut(s) 1108
BstACI GRCGYC 1 cut(s) 51
BstAUI TGTACA 2 cut(s) 831, 1021
BstBAI YACGTR 1 cut(s) 1367
BstC8I GCNNGC 8 cut(s) 113, 477, 544, 1061, 1077, 1848, 1863, 1951
BstDEI CTNAG 6 cut(s) 68, 383, 437, 557, 1121, 1985
BstDSI CCRYGG 2 cut(s) 1149, 1775
BstF5I GGATG 3 cut(s) 742, 1421, 1967
BstFNI CGCG 2 cut(s) 194, 1448
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 196, 257
BstKTI GATC 7 cut(s) 207, 294, 443, 718, 723, 1008, 1546
BstMAI GTCTC 2 cut(s) 80, 547
BstMBI GATC 7 cut(s) 204, 291, 440, 715, 720, 1005, 1543
BstMCI CGRYCG 1 cut(s) 1073
BstMWI GCNNNNNNNGC 3 cut(s) 191, 296, 305
BstNI CCWGG 2 cut(s) 1223, 1868
BstNSI RCATGY 2 cut(s) 1400, 1490
BstSCI CCNGG 5 cut(s) 54, 285, 336, 1221, 1866
BstSFI CTRYAG 2 cut(s) 582, 1032
BstSLI GKGCMC 2 cut(s) 327, 532
BstSNI TACGTA 1 cut(s) 1367
BstUI CGCG 2 cut(s) 194, 1448
BstV1I GCAGC 4 cut(s) 596, 628, 631, 1958
BstV2I GAAGAC 1 cut(s) 778
BstX2I RGATCY 1 cut(s) 1005
BstXI CCANNNNNNTGG 4 cut(s) 224, 776, 889, 1634
BstYI RGATCY 1 cut(s) 1005
BstZ17I GTATAC 1 cut(s) 1083
BsuRI GGCC 6 cut(s) 161, 336, 530, 1155, 1538, 1865
BtgI CCRYGG 2 cut(s) 1149, 1775
BtsCI GGATG 3 cut(s) 742, 1421, 1967
BtsI GCAGTG 2 cut(s) 470, 553
BtsIMutI CAGTG 2 cut(s) 470, 553
Cac8I GCNNGC 8 cut(s) 113, 477, 544, 1061, 1077, 1848, 1863, 1951
CaiI CAGNNNCTG 1 cut(s) 1867
CciI TCATGA 1 cut(s) 613
CfoI GCGC 2 cut(s) 196, 257
Cfr10I RCCGGY 2 cut(s) 111, 2082
Cfr13I GGNCC 6 cut(s) 335, 528, 529, 1153, 1864, 2080
Csp6I GTAC 9 cut(s) 126, 832, 1022, 1048, 1274, 1364, 1419, 1457, 1750
CspAI ACCGGT 1 cut(s) 2082
CviQI GTAC 9 cut(s) 126, 832, 1022, 1048, 1274, 1364, 1419, 1457, 1750
DdeI CTNAG 6 cut(s) 68, 383, 437, 557, 1121, 1985
DpnI GATC 7 cut(s) 206, 293, 442, 717, 722, 1007, 1545
DpnII GATC 7 cut(s) 204, 291, 440, 715, 720, 1005, 1543
DraI TTTAAA 2 cut(s) 601, 2097
DraIII CACNNNGTG 1 cut(s) 23
Eam1104I CTCTTC 1 cut(s) 1108
EarI CTCTTC 1 cut(s) 1108
EciI GGCGGA 1 cut(s) 2010
Eco105I TACGTA 1 cut(s) 1367
Eco130I CCWWGG 8 cut(s) 217, 328, 837, 1149, 1214, 1560, 1627, 1775
Eco147I AGGCCT 1 cut(s) 1538
Eco24I GRGCYC 2 cut(s) 310, 532
Eco47I GGWCC 1 cut(s) 2080
Eco47III AGCGCT 1 cut(s) 256
EcoO109I RGGNCCY 2 cut(s) 529, 1864
EcoRI GAATTC 2 cut(s) 1209, 1348
EcoRII CCWGG 2 cut(s) 1221, 1866
EcoT14I CCWWGG 8 cut(s) 217, 328, 837, 1149, 1214, 1560, 1627, 1775
EcoT22I ATGCAT 1 cut(s) 685
EcoT38I GRGCYC 2 cut(s) 310, 532
ErhI CCWWGG 8 cut(s) 217, 328, 837, 1149, 1214, 1560, 1627, 1775
FaqI GGGAC 1 cut(s) 62
FblI GTMKAC 2 cut(s) 1082, 1230
Fnu4HI GCNGC 6 cut(s) 420, 585, 642, 645, 1446, 1947
FokI GGATG 3 cut(s) 749, 1428, 1974
FriOI GRGCYC 2 cut(s) 310, 532
Fsp4HI GCNGC 6 cut(s) 420, 585, 642, 645, 1446, 1947
FspBI CTAG 5 cut(s) 476, 543, 1215, 1325, 1781
GlaI GCGC 2 cut(s) 195, 256
GluI GCNGC 6 cut(s) 420, 585, 642, 645, 1446, 1947
GsaI CCCAGC 1 cut(s) 139
GsuI CTGGAG 1 cut(s) 1008
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 6 cut(s) 161, 336, 530, 1155, 1538, 1865
HapII CCGG 6 cut(s) 55, 112, 287, 338, 1797, 2083
HhaI GCGC 2 cut(s) 196, 257
Hin1I GRCGYC 1 cut(s) 51
Hin6I GCGC 2 cut(s) 194, 255
HinP1I GCGC 2 cut(s) 194, 255
HincII GTYRAC 2 cut(s) 230, 1979
HindII GTYRAC 2 cut(s) 230, 1979
HpaII CCGG 6 cut(s) 55, 112, 287, 338, 1797, 2083
HphI GGTGA 4 cut(s) 151, 1403, 1576, 2057
Hpy188III TCNNGA 4 cut(s) 614, 1313, 1378, 1797
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 5 cut(s) 668, 1319, 1686, 1829, 2030
HpyCH4III ACNGT 5 cut(s) 190, 916, 1261, 1461, 1976
HpyCH4IV ACGT 3 cut(s) 51, 212, 1366
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 296, 305
HpyF3I CTNAG 6 cut(s) 68, 383, 437, 557, 1121, 1985
HpySE526I ACGT 3 cut(s) 51, 212, 1366
Hsp92I GRCGYC 1 cut(s) 51
HspAI GCGC 2 cut(s) 194, 255
Kpn2I TCCGGA 1 cut(s) 1796
KroI GCCGGC 1 cut(s) 111
KroNI GCCGGC 1 cut(s) 113
Kzo9I GATC 7 cut(s) 204, 291, 440, 715, 720, 1005, 1543
LmnI GCTCC 4 cut(s) 313, 461, 989, 1969
Lsp1109I GCAGC 4 cut(s) 596, 628, 631, 1958
LweI GCATC 1 cut(s) 1306
MaeI CTAG 5 cut(s) 476, 543, 1215, 1325, 1781
MaeII ACGT 3 cut(s) 51, 212, 1366
MaeIII GTNAC 1 cut(s) 2085
MalI GATC 7 cut(s) 206, 293, 442, 717, 722, 1007, 1545
MboI GATC 7 cut(s) 204, 291, 440, 715, 720, 1005, 1543
MboII GAAGA 5 cut(s) 308, 778, 952, 1095, 1907
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 1 cut(s) 1005
MhlI GDGCHC 5 cut(s) 310, 327, 532, 1308, 1974
MluCI AATT 9 cut(s) 9, 795, 993, 1157, 1184, 1209, 1348, 1355, 1549
MlyI GAGTC 3 cut(s) 586, 1673, 1802
MmeI TCCRAC 3 cut(s) 342, 478, 1705
Mph1103I ATGCAT 1 cut(s) 685
MroI TCCGGA 1 cut(s) 1796
MroNI GCCGGC 1 cut(s) 111
MseI TTAA 3 cut(s) 600, 1055, 2096
MslI CAYNNNNRTG 1 cut(s) 330
MspA1I CMGCKG 1 cut(s) 1075
MspI CCGG 6 cut(s) 55, 112, 287, 338, 1797, 2083
MspR9I CCNGG 5 cut(s) 56, 287, 338, 1223, 1868
MunI CAATTG 1 cut(s) 993
Mva1269I GAATGC 1 cut(s) 853
MvaI CCWGG 2 cut(s) 1223, 1868
MvnI CGCG 2 cut(s) 194, 1448
MwoI GCNNNNNNNGC 3 cut(s) 191, 296, 305
NaeI GCCGGC 1 cut(s) 113
NciI CCSGG 3 cut(s) 56, 287, 338
NcoI CCATGG 2 cut(s) 1149, 1775
NdeII GATC 7 cut(s) 204, 291, 440, 715, 720, 1005, 1543
NgoMIV GCCGGC 1 cut(s) 111
NheI GCTAGC 2 cut(s) 475, 542
NlaIV GGNNCC 4 cut(s) 309, 389, 530, 1220
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 2 cut(s) 1400, 1490
OliI CACNNNNGTG 1 cut(s) 330
PagI TCATGA 1 cut(s) 613
PceI AGGCCT 1 cut(s) 1538
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 853
PdiI GCCGGC 1 cut(s) 113
PfeI GAWTC 8 cut(s) 144, 501, 563, 610, 760, 1117, 1145, 1771
PinAI ACCGGT 1 cut(s) 2082
PkrI GCNGC 6 cut(s) 421, 586, 643, 646, 1447, 1948
PleI GAGTC 3 cut(s) 586, 1672, 1801
PpsI GAGTC 3 cut(s) 586, 1672, 1801
Ppu21I YACGTR 1 cut(s) 1367
Psp6I CCWGG 2 cut(s) 1221, 1866
PspFI CCCAGC 1 cut(s) 135
PspGI CCWGG 2 cut(s) 1221, 1866
PspN4I GGNNCC 4 cut(s) 309, 389, 530, 1220
PspOMI GGGCCC 1 cut(s) 528
PspPI GGNCC 6 cut(s) 335, 528, 529, 1153, 1864, 2080
PstI CTGCAG 1 cut(s) 586
PstNI CAGNNNCTG 1 cut(s) 1867
PsuI RGATCY 1 cut(s) 1005
RsaI GTAC 9 cut(s) 127, 833, 1023, 1049, 1275, 1365, 1420, 1458, 1751
RsaNI GTAC 9 cut(s) 126, 832, 1022, 1048, 1274, 1364, 1419, 1457, 1750
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 3 cut(s) 600, 1055, 2096
SatI GCNGC 6 cut(s) 420, 585, 642, 645, 1446, 1947
Sau3AI GATC 7 cut(s) 204, 291, 440, 715, 720, 1005, 1543
Sau96I GGNCC 6 cut(s) 335, 528, 529, 1153, 1864, 2080
ScaI AGTACT 1 cut(s) 127
SchI GAGTC 3 cut(s) 586, 1673, 1802
ScrFI CCNGG 5 cut(s) 56, 287, 338, 1223, 1868
SduI GDGCHC 5 cut(s) 310, 327, 532, 1308, 1974
SfaNI GCATC 1 cut(s) 1306
SfcI CTRYAG 2 cut(s) 582, 1032
SinI GGWCC 1 cut(s) 2080
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 2 cut(s) 258, 1001
SmoI CTYRAG 2 cut(s) 258, 1001
SnaBI TACGTA 1 cut(s) 1367
SpeI ACTAGT 1 cut(s) 1324
Sse9I AATT 9 cut(s) 9, 795, 993, 1157, 1184, 1209, 1348, 1355, 1549
SseBI AGGCCT 1 cut(s) 1538
SsiI CCGC 6 cut(s) 420, 1073, 1193, 1400, 1446, 1995
SspMI CTAG 5 cut(s) 476, 543, 1215, 1325, 1781
StuI AGGCCT 1 cut(s) 1538
StyD4I CCNGG 5 cut(s) 54, 285, 336, 1221, 1866
StyI CCWWGG 8 cut(s) 217, 328, 837, 1149, 1214, 1560, 1627, 1775
TaaI ACNGT 5 cut(s) 190, 916, 1261, 1461, 1976
TaiI ACGT 3 cut(s) 54, 215, 1369
TaqI TCGA 4 cut(s) 1111, 1651, 1683, 1918
TasI AATT 9 cut(s) 9, 795, 993, 1157, 1184, 1209, 1348, 1355, 1549
TatI WGTACW 5 cut(s) 125, 831, 1021, 1047, 1418
TauI GCSGC 2 cut(s) 422, 1448
TfiI GAWTC 8 cut(s) 144, 501, 563, 610, 760, 1117, 1145, 1771
Tru1I TTAA 3 cut(s) 600, 1055, 2096
Tru9I TTAA 3 cut(s) 600, 1055, 2096
TscAI CASTG 2 cut(s) 477, 553
TseI GCWGC 4 cut(s) 584, 641, 644, 1946
TspDTI ATGAA 8 cut(s) 762, 916, 1095, 1301, 1467, 1606, 1772, 1908
TspGWI ACGGA 3 cut(s) 300, 1889, 1982
TspRI CASTG 2 cut(s) 477, 553
VneI GTGCAC 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 2080
XapI RAATTY 3 cut(s) 1209, 1348, 1355
XceI RCATGY 2 cut(s) 1400, 1490
XmaJI CCTAGG 1 cut(s) 1214
XmiI GTMKAC 2 cut(s) 1082, 1230
XspI CTAG 5 cut(s) 476, 543, 1215, 1325, 1781
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.