Rroxscaffold_1G00014410

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000618
Physical Location & Seq
Forward (+)
17804691 .. 17805387
697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00014410.1

Sequence Viewer

Length: 387 bp
ATGTTTATCGCTGGAATTATCAAGTATGGAGAGAGGACTTGGGTCCTGCGGTCTACAACCAGCGATAGATATAGAAACTTGTCGGCACCTAATCCTGATCATGGTAATGCTCATCTTGGTTTCCGCAATGATGCCGAGGAAACAGAAGTAGAGGATCTAAGAAACAGTTCTTCAGAAGATTACTTCGAAGTAGTAGAGATTGAGCTTGGTCTTATGTATGATTTGCTGTACACAAAGGCAACCATGATATACTCTTTCAAAGCTATTACTCCTGAACTATATGCCATTGCCGTACTAGCTACTTTCCACAGACTGGACACTATACTGGTTAAGCAGGCAAAAGAATATGCTCGCCTGTTGCATCTTCAAAGGAATCAGTTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

128

Amino Acids

14.86

Weight (kDa)

5.53

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 1 - 38 3.3e-06 Domain of unknown function (DUF4220)
DUF4220 PF13968 55 - 108 1e-11 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 85
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 1 cut(s) 53
AciI CCGC 2 cut(s) 49, 124
AclWI GGATC 1 cut(s) 162
AcuI CTGAAG 1 cut(s) 156
AfaI GTAC 2 cut(s) 230, 294
AfiI CCNNNNNNNGG 2 cut(s) 101, 313
AgsI TTSAA 2 cut(s) 259, 368
AluBI AGCT 3 cut(s) 205, 263, 299
AluI AGCT 3 cut(s) 205, 263, 299
AlwI GGATC 1 cut(s) 162
Asp700I GAANNNNTTC 2 cut(s) 166, 377
AspS9I GGNCC 1 cut(s) 43
AsuII TTCGAA 1 cut(s) 186
AvaII GGWCC 1 cut(s) 43
BanI GGYRCC 1 cut(s) 85
BceAI ACGGC 1 cut(s) 275
BclI TGATCA 1 cut(s) 97
BfaI CTAG 1 cut(s) 296
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 2 cut(s) 44, 87
BmsI GCATC 2 cut(s) 121, 370
BoxI GACNNNNGTC 1 cut(s) 41
Bpu14I TTCGAA 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 135
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 313
Bse1I ACTGG 2 cut(s) 318, 330
Bse3DI GCAATG 2 cut(s) 133, 285
BseDI CCNNGG 1 cut(s) 135
BseLI CCNNNNNNNGG 2 cut(s) 101, 313
BseMI GCAATG 2 cut(s) 133, 285
BseNI ACTGG 2 cut(s) 318, 330
BshNI GGYRCC 1 cut(s) 85
BslI CCNNNNNNNGG 2 cut(s) 101, 313
Bsp119I TTCGAA 1 cut(s) 186
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 2 cut(s) 97, 154
BspACI CCGC 2 cut(s) 49, 124
BspLI GGNNCC 2 cut(s) 44, 87
BspPI GGATC 1 cut(s) 162
BspT104I TTCGAA 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 85
BsrDI GCAATG 2 cut(s) 133, 285
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 2 cut(s) 318, 330
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 2 cut(s) 97, 154
Bst4CI ACNGT 1 cut(s) 167
BstAUI TGTACA 1 cut(s) 228
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 336, 352
BstDEI CTNAG 1 cut(s) 158
BstKTI GATC 2 cut(s) 100, 157
BstMBI GATC 2 cut(s) 97, 154
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstPAI GACNNNNGTC 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
Cac8I GCNNGC 2 cut(s) 336, 352
Cfr13I GGNCC 1 cut(s) 43
Csp6I GTAC 2 cut(s) 229, 293
CviAII CATG 2 cut(s) 101, 244
CviJI RGCY 3 cut(s) 205, 263, 299
CviKI_1 RGCY 3 cut(s) 205, 263, 299
CviQI GTAC 2 cut(s) 229, 293
DdeI CTNAG 1 cut(s) 158
DpnI GATC 2 cut(s) 99, 156
DpnII GATC 2 cut(s) 97, 154
Eco47I GGWCC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 156
EcoO109I RGGNCCY 1 cut(s) 43
FaeI CATG 2 cut(s) 104, 247
FatI CATG 2 cut(s) 100, 243
FbaI TGATCA 1 cut(s) 97
FblI GTMKAC 1 cut(s) 53
FspBI CTAG 1 cut(s) 296
Hin1II CATG 2 cut(s) 104, 247
HinfI GANTC 1 cut(s) 373
Hpy166II GTNNAC 2 cut(s) 54, 231
Hpy188I TCNGA 1 cut(s) 175
Hpy188III TCNNGA 2 cut(s) 95, 272
Hpy8I GTNNAC 2 cut(s) 54, 231
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4V TGCA 1 cut(s) 361
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 1 cut(s) 158
Hsp92II CATG 2 cut(s) 104, 247
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 2 cut(s) 97, 154
LpnPI CCDG 8 cut(s) 59, 73, 108, 285, 299, 311, 320, 368
LweI GCATC 2 cut(s) 121, 370
MaeI CTAG 1 cut(s) 296
MalI GATC 2 cut(s) 99, 156
MboI GATC 2 cut(s) 97, 154
MboII GAAGA 3 cut(s) 162, 188, 356
MflI RGATCY 1 cut(s) 154
MluCI AATT 1 cut(s) 15
MnlI CCTC 3 cut(s) 27, 130, 145
MroXI GAANNNNTTC 2 cut(s) 166, 377
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 296
NdeII GATC 2 cut(s) 97, 154
NlaIII CATG 2 cut(s) 104, 247
NlaIV GGNNCC 2 cut(s) 44, 87
NmeAIII GCCGAG 1 cut(s) 160
NspV TTCGAA 1 cut(s) 186
PdmI GAANNNNTTC 2 cut(s) 166, 377
PfeI GAWTC 1 cut(s) 373
PflMI CCANNNNNTGG 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 43
PshAI GACNNNNGTC 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 44, 87
PspPI GGNCC 1 cut(s) 43
PspPPI RGGWCCY 1 cut(s) 43
PsuI RGATCY 1 cut(s) 154
RsaI GTAC 2 cut(s) 230, 294
RsaNI GTAC 2 cut(s) 229, 293
RseI CAYNNNNRTG 1 cut(s) 105
SaqAI TTAA 1 cut(s) 330
Sau3AI GATC 2 cut(s) 97, 154
Sau96I GGNCC 1 cut(s) 43
SetI ASST 4 cut(s) 91, 207, 265, 301
SfaNI GCATC 2 cut(s) 121, 370
SfuI TTCGAA 1 cut(s) 186
SinI GGWCC 1 cut(s) 43
SmiMI CAYNNNNRTG 1 cut(s) 105
Sse9I AATT 1 cut(s) 15
SsiI CCGC 2 cut(s) 49, 124
SspMI CTAG 1 cut(s) 296
TaaI ACNGT 1 cut(s) 167
TaqI TCGA 1 cut(s) 186
TasI AATT 1 cut(s) 15
TatI WGTACW 1 cut(s) 228
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
Van91I CCANNNNNTGG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 43
XmiI GTMKAC 1 cut(s) 53
XmnI GAANNNNTTC 2 cut(s) 166, 377
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.